ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Shuang Gao,Yaguang Wang,Huicong Feng,Zipeng Zhou,Yang Cui,Mingyu Bai,Biaobiao Wang,Yingqi Liu,Jidi Liu,Chao Wu,Xifan Mei,Yang Qiu,He Tian
Spinal cord injury (SCI) is a devastating condition from which there is limited recovery of motor function. Inhibiting oxidative stress and inflammation is an effective strategy for treating SCI. The polysaccharide component of Schisandra chinensis is receiving increasing attention due to its potential in neuroprotection. Based on our previous research, we synthesized an Schisandra chinensis polysaccharide-zinc complex (SPZn) for synergistic treatment of SCI. However, poor blood-spinal cord barrier permeability limited the efficacy of SPZn. Microglia are immune cells that are inherent to the spinal cord and possess intrinsic targeting ability combined with immune evasion capacity, making them an ideal drug delivery system for SCI. Therefore, in this study, we selected microglia membranes to encapsulate SPZn (M-SPZn) for the treatment of SCI. The nanovesicle (M-SPZn) designed by us can target the site of spinal cord trauma and has good biosafety. The results, both in vivo and in vitro, showed that M-SPZn is more effective at alleviating oxidative stress and inhibiting inflammation. Moreover, M-SPZn reduced cell death and promoted motor function recovery in mice after SCI. In summary, M-SPZn has multiple roles in treating SCI and has a good usage potential.
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高影响因子
Frontiers in Bioscience-Landmark
·
2023
·Vol. 28
·期 8
·177
·
IF 3.1
Pingping Lv,Huirong Zhang,Meiling Tai,Biao Che,Dan Yu,Ying Zhang,Haodong Li,Xianyao Meng,Miaomiao Guo,Li Li
Background: Propionibacterium acnes causes upregulation of inflammatory factors, such as cycloxygenase-2, prostaglandin E2, interleukin-1β, and tumor necrosis factor-alpha, increased levels of reactive oxygen species (ROS) and inward flow of calcium ions. This causes increased levels of the antimicrobial peptide LL-37 and inflammation of the skin, leading to redness, swelling, itching and other symptoms. Schisandra chinensis fruit oil (SCO) is rich in lignan active ingredients with various antioxidant and anti-inflammatory properties. Methods: In this study, SCO is obtained by supercritical CO2 fluid extraction. SCO’s anti-inflammatory actions were investigated using P. acnes-induced inflammation HaCaT cells model. A method based on reversed-phase high-pressure liquid chromatography with a diode array detector was developed and validated for the simultaneous detection of five lignan components. Levels of inflammatory factors and LL-37 were measured by ELISA kit and western blot respectively. Ca2+ and ROS levels detected by flow cytometry. Results: The experimental results show that the contents of schisanol A, schisanol B, schisanin A, schisanin B, and schisanin C were 33.89 ± 0.24, 14.89 ± 0.45, 8.92 ± 0.02, 29.14 ± 0.67, and 4.74 ± 0.09 mg/g, respectively. Studies have demonstrated that SCO can alleviate skin inflammation by inhibiting the COX-2/PGE2 and NF-κB signalling pathway. In addition, SCO can inhibit ROS production, significantly block inward Ca2+ flow, alleviate cell damage, and modulate the content of the antimicrobial peptide LL-37. Conclusions: In summary, our study elucidated the anti-inflammatory activity of SCO in a cell model and provided a scientific basis for its application as a raw material in skin care.
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高影响因子
PHYTOMEDICINE
·
2024
·
IF 6.7
Jinyu Wang,Aijuan Zheng,Fei Lin,Xiaozhuo Zhang,Kaifang Shi,Tingxu Yan,Ying Jia
Background Schisandra chinensis lignan (SCL), a major active component of the traditional functional Chinese medicine Schisandra chinensis , has been reported to have antidepressant effects. Its mechanisms include alleviating intestinal barrier injury (IBI) by resolving intestinal microflora, anti-inflammation, and neuroprotection. SCL also regulates endogenous cannabinoid system, and it is closely related to the onset and development of depression. Purpose We investigated a new treatment strategy for depression, i.e., alleviating IBI by regulating the endogenous cannabinoid system for antidepressant effects, as well as conducted in-depth research to explore the specific mechanism. Methods Behavioral analysis was conducted to detect the occurrence of depressive-like behavior in C57BL/6 mice. We used hematoxylin-eosin staining, periodic acid-Schiff staining, and immunofluorescence to evaluate IBI. Network pharmacology and Western blotting (WB) were used to predict and confirm that the amelioration effect of SCL was associated with anti-inflammation and anti-apoptosis. Combined with the levels of anandamide (AEA) and 2-arachidonoylglycerol (2-AG), we conducted the Pearson analysis between the AEA, 2-AG levels and the major targets identified and validated by network pharmacology and WB. Subsequently, URB-597, a fatty acid amide hydrolase (FAAH) antagonist with an AEA hydrolase-inhibiting effect, was administered to the mice, and behavioral analysis and apoptotic proteins were verified. Plasma endocannabinoid levels after URB-597 supplementation were measured via 6470 Triple Quadrupole LC/MS. Finally, the cannabinoid receptor type 2 (CB2R) antagonist AM630 was administered to mice, and immunofluorescence and WB were performed to assess the proteins of IBI and anti-inflammation. Results The study demonstrated that SCL alleviated depressive-like behaviours and ameliorated IBI. Network pharmacology and WB confirmed that the improvement of IBI was related to the anti-inflammatory and anti-apoptotic pathways. Pearson results showed that AEA levels were positively correlated with inflammation and apoptosis, with a greater contribution to apoptosis. In-depth studies validated that the URB-597 administration reversed the positive effects of SCL on depressive-like behavior and anti-apoptosis. Similarly, URB-597 counteracted AEA levels reduced by SCL and decreased 2-AG levels. Furthermore, AM630 supplementation antagonized SCL's effect of improving IBI by reactivating the MAPK/NF-κB inflammation pathway. Conclusion Overall, SCL, in collaboration with the endogenous cannabinoid system regulated by SCL, alleviates depression associated IBI. The specific mechanism involes SCL decreasing AEA levels to inhibit colon tissue cell apoptosis by up-regulating FAAH. Simultaneously, it directly triggers CB2R to reduce inflammation responses, further alleviating IBI.
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高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2022
·
IF 3.571
Aimin Wang,Minjie Shi,Junpeng Xing,Shu Liu,Zhiqiang Liu,Fengrui Song
Traditional Chinese medicine (TCM) plays a synergistic and comprehensive pharmacodynamic role of multi-channel and multi-target through its multi-components, showing unique therapeutic advantages in chronic and multi-gene complex diseases. Herb pair is a unique combination of two relatively fixed herbs, which embodies the integrity of TCM theory. In this study, untargeted fecal metabolomics based on MS was used to investigate the action mechanism of Radix ginseng and Schisandra chinensis (GS) herb pair on the complex disease of Alzheimer's disease (AD), and further analyze the therapeutic effects of small molecular components and saccharides of GS on AD. Quantitative analysis of bile acids (BAs) and short-chain fatty acids (SCFAs) further verified the conclusion of untargeted metabolomics. The results of the pharmacodynamics evaluation showed that the AD model was successfully constructed, and each TCM group had a different degree of improvement compared with the AD group. PCA analysis based on untargeted fecal metabolomics showed that the metabolic disorders in AD rats changed significantly over time, and there were different degrees of callback in each TCM group. The result indicated that the GS herb pair can regulate metabolic disorders of AD. Further analysis of therapeutic biomarkers showed that GS mainly regulated the metabolism of bile acid biosynthesis, sphingolipid metabolism, porphyrin and chlorophyll metabolism, etc. to treat AD. This study will help to further understand the pathogenesis of AD from metabolomics, and provide beneficial support for the further study of GS and the clinical treatment of complex diseases with TCM.
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Waste and Biomass Valorization
·
2024
·
IF 2.6
Zhao Zijia,Gao Yan,Yuan Min,Ye Cai,Guo Yingxue,Zhao Yanli,Li Jinlian,Cui Jiwen,Dong Tianwei,Wu Dongmei
A significant quantity of residues will produce when volatile oil in Schisandra chinensis fruit is extracted by steam distillation. It is important to investigate the residues for potential use. Currently, there is an increasing demand for anti-photoaging products due to the skin damage caused by UV radiation. In this paper, the ingredients of the residues were investigated, and the anti-photoaging activities of residues extracts were studied. The results showed that the residues still contained notable amounts of lignans, flavonoids, and organic acids. In the extracted fraction of dichloromethane (DE), the residues contained higher amounts of schizandrol B and schisantherin A compared to the fresh fruit. The levels were particularly high at 17.39 ± 0.02 and 52.58 ± 0.04 mg/ml, respectively. By lowering the amount of matrix metalloproteinase-1 (MMP-1), reactive oxygen species (ROS) accumulation, and type I collagen (COL-I) loss in fibroblasts, the residues showed targeted benefits in the fight against skin photoaging. In particular, the DE-R phase of residues was very successful in raising the level of COL-I, matching or even exceeding that of retinol (ROL) and undistilled fresh fruits. Protocatechuic acid, schizandrol A, schizandrol B, schisantherinA, schisandrin A and schizandrin B were found to have a significant correlation with the anti-photoaging ability of residues. Particularly, there was a strong and significant correlation between an increase in COL-I and schizandrol B (|ρ| = 0.829*) and schisantherin A (|ρ| = 0.928**), and the distillation procedure has the potential to increase the amount of schizandrol B and schisantherin A in the residues. Graphical
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Current Research in Food Science
·
2025
·
IF 7
Yan-Yu Chi,Jun-Yan Xiang,Hui-Min Li,Hao-Yu Shi,Ke Ning,Hongyu Xiang,Qiuhong Xie
Alcohol-associated liver disease (ALD) has emerged as a global public health concern, and gut microbiota plays a decisive role in its pathogenesis. Schisandra chinensis , a functional edible berry, exhibits hepatoprotective properties. However, the preventive effects of its main active component lignans against ALD, as well as their connections with gut microbiota and intestinal metabolites remain unclear. This study employed a mouse model of ALD induced by chronic-binge ethanol feeding to evaluate the hepatoprotective effects of lignans-rich extract of S. chinensis (SCL) interventions at different doses. 16S rDNA sequencing and metabolomic analysis were used to investigate microbial and metabolite profiles in intestinal contents. The hepatoprotective activity of key metabolite responding to SCL was further verified by in vivo and in vitro experiments. The results demonstrated that SCL effectively restored intestinal barrier function and alleviated alcohol-induced hepatic injury. SCL modulated intestinal tryptophan (Trp) metabolism and restored indole-3-acetic acid (IAA) levels. Concurrently, SCL reshaped the gut microbiota composition, enriched L . reuteri . Further in vivo and in vitro investigations demonstrated that SCL-mediated enrichment of L. reuteri contributed to intestinal IAA restoration which reactivated the ethanol-suppressed AHR pathway, enhanced intestinal barrier integrity, reduced lipopolysaccharide translocation into circulation and ultimately attenuates liver injury. Collectively, SCL prevent ALD by regulating intestinal microbiota and Trp metabolism. This study provides a scientific foundation for the development of S. chinensis as a functional food for ALD prevention.
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高影响因子
Science of Advanced Materials
·
2023
·Vol. 15
·期 2
·243-255
·
IF 0.9
Chen Aimin,Hu Ruihua
Schisandra polysaccharide (SCHP) was utilized as the target drug, and Zein (corn prolamine) was utilized as the drug carrier. First, SCHP/Zein nanoparticles (Zein-SCHP NPs) were prepared. Then, a polydopamine (PDP) coating was utilized to enhance the stability and tumor targeting of
Zein. Finally, the folic acid (FA)-targeting ligand was covalently attached to the PDP-modified Zein-SCHP@PDP NP surface to form a highly pH-sensitive NP (Zein-SCHP@PDP-FA NPs) with active/passive targeting. In the experiment, apart from physical characterization of Zein-SCHP-based nanomaterials,
the in vitro cytotoxicity and cell uptake properties of these materials were tested. In addition, an ovarian cancer cell line, SKOV3, was taken as the research object to explore the photodynamic-mediated killing effect of Zein-SCHP@PDP-FA NPs on this cancer cell. The results demonstrated
that the average particle size of Zein-SCHP@PDP-FA NPs was (379.68±1.36) nm, the zeta potential was (−43.68±1.67) mV, the encapsulation efficiency was (97.86±1.38)%, and the drug loading was (11.75±0.16)%. Transmission electron microscope (TEM) suggested that
the Zein-SCHP@PDP-FA NPs were spherical, and there was no adhesion between the particles. Differential scanning calorimetry (DSC) and far infrared (FIR) results indicated that SCHP existed in the NPs in the crystal structure and proved that the PDP and FA were successfully modified. The in
vitro release of the drug showed that Zein-SCHP@PDP-FA NPs had sustained release, and the CCK-8 test showed that Zein-SCHP@PDP-FA NPs were highly cytotoxic and could notably reduce the IC50 values of SCHP. Furthermore, in vitro uptake experiments revealed that Zein-SCHP@PDP-FA
NPs exhibited strong specific recognition in cells expressing different folate receptors. Compared with Zein-SCHP NPs and Zein-SCHP@PDP NPs, Zein-SCHP@PDP-FA NPs combined with photodynamic therapy enhanced reactive oxygen species (ROS) production in cells, downregulated hypoxia-inducible factor
(HIF)-1α and interleukin (IL)-6 proteins, and exerted more destructive effects on SKOV3 cells.
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
Yaning Wang,Zihui Cai,Quan Zhou,Anquan Yang,Min Xie,Yangruili Niu,Litao Wang,Yujie Fu
The growing demand for sustainable technologies has inspired interest in green extraction and delivery systems for plant-derived natural products. This study developed a hydrophobic deep eutectic solvent (HDES)-based nanoemulsion for efficient extraction and delivery of lignans from Schisandra chinensis . Through systematic screening, a HDES composed of menthol and octanoic acid (Men:C 8 ) was selected for extraction and subsequent preparation. After optimizing extraction parameters (solid-liquid ratio 40 mg/mL, extraction temperature 60°C, extraction time 40 min), the HDES demonstrated superior extraction efficiency (6.497 ± 0.008 mg/mL), representing a 1.12–2.08 fold improvement compared to water or conventional organic solvents. The extract-containing HDES was formulated into a nanoemulsion (HDES-NE) that exhibited excellent stability under various environmental conditions (pH, temperature, centrifugal force, storage time). The preparation conditions were optimized to establish optimal process parameters (oil-water ratio 2:17, homogenization time 3 min, ultrasonic power 360 W). The resulting nanoemulsion exhibited nanoscale characteristics (average particle size 115.78 nm, PDI < 0.3, and a zeta potential of −18.11 mV), with spherical morphology directly visualized by TEM, and no phase separation or precipitation. HDES-NE showed significant tyrosinase inhibition (IC 50 0.26 ± 0.04 mg/mL; 97.73% inhibition at 4 mg/mL), substantial UVB protection (SPF = 17.58 ± 0.27), and antimicrobial activity against skin pathogens, including Staphylococcus aureus and Cutibacterium acnes . This integrated approach, combining green extraction with nano-delivery, offers an environmentally friendly alternative for developing natural bioactive products.
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高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
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IF 4
Wenrui Li,Guojuan Yang,Yike Dang,Qinyuan Wang,Fan Li,Ruihong Wang,Yongning Wu,Guoliang Li
Lignans from Schisandra chinensis have strong anti-hepatotoxic, antioxidant, and anti-tumor properties. Herein, a multivariate metal-organic framework (MTV-MOF) was synthesized with biphenyl dicarboxylic acid, 3,5-diaminobenzoic acid (DABA), and aluminum-based metal salts, introduced 1,8-dihydroxynaphthalene (AM) as a porous coating, constructed a new magnetic material (Fe 3 O 4 @DUT-5-DABA@AM) and utilized in magnetic solid-phase extraction (MSPE) as potent adsorbents to adsorb Schizandrol A, Schizandrol B, Schisantherin A, Schisandrin A, and Schizandrin B, five lignans. Prepared materials were characterized using FT-IR, XRD, TEM, SEM, VSM, zeta and nitrogen adsorption-desorption analyses. At the same time, their adsorption kinetics and isothermal adsorption lines were also evaluated. Due to electrostatic interactions, π stacking interactions, and hydrogen bonding, the composite exhibited excellent extraction capability for lignans. In conjunction with HPLC-MS, the MSPE approach demonstrated promising linearity (R 2 ≥ 0.9992) and low detection limits (0.024–0.2 μg·kg −1 ) for lignans under optimal circumstances. The approach efficaciously quantified lignans in S. chinensis extract, attaining recoveries of 93.4–116.6 % with relative standard deviations ≤ 6.3 %. Compared with traditional methods, the magnetic separation method proposed in this study is simple, economical, and practical with low solvent consumption, which has unique advantages in the analysis of natural active ingredients.
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高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Xiaoyu Wu,Jiajia Wei,Rongzheng Deng,Jinyu Wang,Bo Wu,Tingxu Yan,Ying Jia
Current therapies for ulcerative colitis (UC) remain inadequate, necessitating novel treatment strategies. This study isolated SCPII-1, a homogeneous pectin polysaccharide (Mw: 278.153 kDa, DM: 57.21 %) from Schisandra chinensis , primarily composed of →4)-α-D-GalpA-(1 → units. In dextran sulfate sodium (DSS)-induced colitis mice, SCPII-1 alleviated symptoms, repaired mucosal ultrastructure (microvilli, mitochondria), and enhanced barrier integrity. Mechanistically, SCPII-1 ameliorated gut dysbiosis in UC mice, notably through the selective enrichment of the beneficial bacterium Akkermansia muciniphila (AKK). Interestingly, SCPII-1 not only promoted the proliferation of AKK but also modulated its tryptophan metabolism, particularly enhancing the production of the tryptophan-derived metabolite indole-3-ethanol (I3E). The beneficial role of SCPII-1-induced microbiota in UC was validated using pseudo-germ-free mice and fecal microbiota transplantation experiments. Moreover, I3E was identified as a key microbiota-derived active metabolite regulated by SCPII-1, and exogenous supplementation of I3E further confirmed its therapeutic efficacy in UC. Importantly, I3E activated the aryl hydrocarbon receptor (AhR), upregulated IL-22 expression, and promoted mucosal repair and immune homeostasis, thereby confirming the protective role of the SCPII-1-AKK-tryptophan metabolism axis in colitis. These findings highlight SCPII-1 as a promising prebiotic agent for UC treatment by modulating AKK-dependent tryptophan metabolism and activating the AhR/IL-22 signaling pathway, offering a novel microbiota-based therapeutic strategy.
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高影响因子
Heliyon
·
2024
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IF 3.4
Yanhua Sheng,Jinxu Dong,Rui Wang,Yikai Wang,Xin Huang,Changbao Chen
Schisandra chinensis is a functional fruit with tonic effect and was widely used by traditional Chinese medicine for treatment and health care. The quality of Schisandra chinensis fruit may vary by different storage condition. In this study, the influence of ambient temperature, humidity, packaging materials and period of storage on the quality of Schisandra chinensis fruits were investigated. The contents of main active components lignans and organic acids were simultaneously determined by ultra-performance liquid chromatography coupled to quadrupole electrostatic field orbitrap high resolution mass spectrometry (UPLC Orbitrap HRMS). The antioxidant activity was determined using DPPH radical scavenging capacity, ABTS + inhibition rate and FRAP value. The correlation of multicomponent and antioxidant activity was analyzed by grey relevance analysis. Taking the changes of multicomponent and antioxidant activity as investigation index, Schisandra chinensis fruits under different storage conditions was comprehensively evaluated. Schisandrol A, malic acid, sorbic acid, schizandrin A, schizandrin B, and schisandrol B were the main effective components of antioxidant activity. Ambient temperature at 5 °C and humidity at 40 % were more suitable for Schisandra chinensis fruits and kraft paper bag was better packaging material. Do not exceed 1 year was the effective storage period. For the safety evaluation, no aflatoxin was detected within the storage period of 2 years, demonstrated the storage was satisfactory. This study provided a reference for the high-quality storage and standardized operating procedures for storage of Schisandra chinensis fruits.
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高影响因子
Journal of Chemistry
·
2022
·
IF 3.241
Yan-hua Sheng,Rui Wang,Yi-kai Wang,Meng-meng Li,Fang-xin Liu,Xin Huang,Chang-bao Chen
Schisandra chinensis fruit is a famous tonic traditional Chinese medicine and healthy food. The chemical components of dried fruits may vary with the initial processing methods of fresh fruits. Therefore, in this study, the influence of drying was evaluated based on targeted metabolomics analysis. The ultrahigh performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-QQQ-MS) was applied in determination of multicomponent including lignans, organic acids, and oligosaccharides. The fresh fruits of Schisandra chinensis were processed by natural drying in the sun, hot air drying and vacuum drying at different temperatures, and vacuum freeze drying, respectively. The contents of lignans, organic acids, and oligosaccharides in different fruit samples were quantified and statistically analyzed by partial least squares discriminant analysis (PLS-DA). The variation of totally 10 lignans, 5 organic acids, and 9 oligosaccharides with different drying process were compared. The results showed that the drying time of natural drying method was much longer than the other methods using instrument. For hot air and vacuum drying samples, most of lignans, organic acids, and oligosaccharides gradually decreased with the increase of temperature. And the appearance of fruit drying at higher temperature was dark red. At the same processing temperature, vacuum drying takes longer time than hot air drying. In addition, organic acid and oligosaccharide contents of vacuum freeze drying treated samples were higher than the other processed samples significantly. According to the targeted metabolomics analysis, the lignans, organic acids, and oligosaccharides markers were screened out for holistic quality evaluation of Schisandra chinensis fruits. In comprehensive consideration of multicomponent, drying time, fruit appearance and storage, and production feasibility, hot air drying at 50°C is more suitable for the process of Schisandra chinensis. The result is expected to provide a scientific basis for the selection of proper method for drying process of fresh Schisandra chinensis fruit.
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高影响因子
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