Translational Cancer Research
·
2019
·
IF 1.07
Guangyuan Sun,Fei Guo,Xueliang Wu,Lei Han,Jun Xue
Background LncRNA cancer susceptibility candidate 9 (CASC9) is up-regulated in various cancers. In this study, we explored the role of CASC9 in colon cancer (CC). Methods The level of CASC9 in CC tissues, adjacent normal tissues, intestinal epithelial cells (HIEC) and CC cell lines was evaluated by qRT-PCR. HCT116 and SW480 CC cells were transfected with siCASC9. The cell growth and cell proliferation makers (Ki-67 and PCNA) were detected by CCK-8, colony formation and western blotting, respectively. The cell apoptosis and cell cycle were determined by flow cytometry. MicroRNA sponged by CASC9 was predicted by starBase and verified by dual-luciferase reporter assay. Further analyses were performed to investigate the role of CASC9 sponging the microRNA in CC cells by transfection or co-transfection of siCASC9 and miR-145-5p inhibitor. Results LncRNA CASC9 was high-expressed in CC cells and tissues, especially in CC at advanced TNM stages. In HCT116 and SW480 cells, knockdown of CASC9 suppressed cell proliferation, promoted cell apoptosis and arrested cell cycle at G0/G1 phase. Furthermore, CASC9 was observed to sponge and regulate the expression of miR-145-5p, which was down-regulated in CC tissues. Moreover, increased cell proliferation and decreased cell apoptosis and arrested cell cycle caused by miR-145-5p inhibitor were abrogated by the knockdown of CASC9. Conclusions LncRNA CASC9 is an oncogene in CC cells through sponging miR-145-5p. The findings in the current study provide a new understanding on CASC9 in CC.
研究领域:
肿瘤学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL RECORD
·
2022
·Vol. 22
·期 11
·e202200139
·
IF 6.935
Suchi Smita Biswas,Soumita Chakraborty,Arunava Saha,Muthusamy Eswaramoorthy
Aqueous electrochemical nitrogen reduction (ENR) to ammonia (NH 3 ) under ambient conditions is considered as an alternative to the energy-intensive Haber-Bosch process for ammonia production. Many metal, non-metal, carbon-based materials along with metal-chalcogenides, metal-nitrides have been explored for their ENR activity. The reported NH 3 production through ENR is still in the micro-gram level and often falls in the range of NH 3 and NO x contaminations from the surrounding. The quantification of NH 3 at very low concentration possess enormous challenge in this field and thus many reported ENR electrocatalysts suffer from reproducibility issue. This review highlights in detail the challenges associated with ENR in aqueous medium and necessitates standardization of protocols to quantify the low concentration of NH 3 free of false-positives. It concludes the prospects of electrochemical NH 3 production through lithium-mediated N 2 reduction.
研究领域:
化学工程
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cell Metabolism
·
2023
·
IF 29
Jie Cheng,Jinxin Yan,Ying Liu,Jiangzhou Shi,Haoyu Wang,Hanyang Zhou,Yinglin Zhou,Tongcun Zhang,Lina Zhao,Xianbin Meng,Haipeng Gong,Xinxiang Zhang,Haichuan Zhu,Peng Jiang
Metabolic alterations in the microenvironment significantly modulate tumor immunosensitivity, but the underlying mechanisms remain obscure. Here, we report that tumors depleted of fumarate hydratase (FH) exhibit inhibition of functional CD8 + T cell activation, expansion, and efficacy, with enhanced malignant proliferative capacity. Mechanistically, FH depletion in tumor cells accumulates fumarate in the tumor interstitial fluid, and increased fumarate can directly succinate ZAP70 at C96 and C102 and abrogate its activity in infiltrating CD8 + T cells, resulting in suppressed CD8 + T cell activation and anti-tumor immune responses in vitro and in vivo. Additionally, fumarate depletion by increasing FH expression strongly enhances the anti-tumor efficacy of anti-CD19 CAR T cells. Thus, these findings demonstrate a role for fumarate in controlling TCR signaling and suggest that fumarate accumulation in the tumor microenvironment (TME) is a metabolic barrier to CD8 + T cell anti-tumor function. And potentially, fumarate depletion could be an important strategy for tumor immunotherapy.
研究领域:
肿瘤学
·
免疫学
·
代谢学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2024
·Vol. 35
·期 26
·1-18
·
IF 2.8
Liang Xiaolu,Wang Zhenyu,Chen Zihao,Jiang Jiabin,Han Jiamei,Wang Yao,Wang Haitao,Pan Zimeng,Sun Jiankun,Ma Jun,Jiang Xuexing,Peng Jubo,Liu Xiang,Gu Xin
Perovskite manganite oxides have attracted considerable attention due to their strong electronic correlation. However, their practical application is challenged by high manufacturing costs and inferior electrical properties. In this study, the sol–gel method was employed to precisely control the Sr content in La 1− x Sr x MnO 3 (LSMO, x = 0.175, 0.195, 0.215, and 0.235) polycrystalline ceramics, the relationship between doping levels and electrical transport properties was systematically investigated. The results show that substituting La 3+ with Sr 2+ can effectively tune the electrical transport properties of LSMO ceramics. X-ray diffraction analysis indicates that all samples exhibit a single-phase perovskite structure with no secondary phases detected. Scanning electron microscopy images reveal that the sample surfaces have good grain connectivity, with grain size increasing as the Sr doping level increases. The resistivity–temperature curves of the samples were measured using the standard four-probe method, and significant differences of electrical transport properties were observed within the range of Sr doping studied. Notably, when the Sr doping level is x = 0.195, the temperature coefficient of resistivity ( TCR ) value of LSMO ceramics increases significantly to 10.3% K −1 at the peak TCR temperature ( T k ) of 296.3 K. In summary, these results offer a theoretical basis and guidance for future development of highly sensitive uncooled infrared bolometers.
研究领域:
材料科学
·
电子工程
✓ 已验证
产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2025
·
IF 5.6
Yiliang Liu,Xiaolu Liang,Yufei Du,Yao Wang,Zihao Chen,Zhenyu Wang,Haitao Wang,Xin Gu,Jiankun Sun,Jing Zhang,Xiang Liu
The influence of sintering duration ( t s = 7, 10, 13 and 16 h) on the microstructure, A-site cation disorder ( σ 2 ), hopping conduction mechanism and temperature coefficient of resistance characteristics of La 0.81 Sr 0.19 MnO 3 polycrystalline ceramics were investigated in this work. By systematically adjusting the sintering duration, it is observed that the A-site cation disorder has decreased from σ 2 = 4.23 × 10 −3 ( t s = 7 h) to σ 2 = 3.71 × 10 −3 ( t s = 13 h). The changes in the A-site cation disorder increase the ratio of Mn 4+ /Mn 3+ , Mn-O bond lengths and angles of perovskite manganite oxides. The hopping conduction mechanism of the ceramics was examined using the variable range hopping model, and the result verified that the dynamics of charge carriers are significantly impacted by variations in cation disorder. All of the scattering processes in electron-electron, electron-phonon, and electron-magnon in La 0.81 Sr 0.19 MnO 3 ceramics were decreased with cation disorder decreasing. The temperature coefficient of resistivity ( TCR ) achieved its maximum value of 11.19 % K −1 at room temperature (298.56 K) when t s = 13 h. The value of TCR has increased by 38.32 % from 8.05 % K −1 ( t s = 10 h) to 11.19 % K −1 ( t s = 13 h). Therefore, the ceramic has great application potential for infrared bolometers.
研究领域:
材料科学
·
物理学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2023
·
IF 6.7
Xiaokun Zhu,Shuaizhao Jin,Xiaohan Yu,Zhiyuan Yu,Xin Gu,Xiaoli Guan,Kaikai Wu,Yixin Yan,Liming Zhao,Jiamei Han,Jiabin Jiang,Xiang Liu,Xiangming Li
In this study, La 0.7 Ag 0.3- x K x MnO 3 (LAKMO, x = 0.15, 0.16, 0.17, 0.18, and 0.19) films with excellent electrical properties and low surface roughness were prepared on SrTiO 3 ( 0 0 1 ) substrates using the spin-coating method. The crystal structures and ion valence states of the LAKMO films were modified by adjusting the doping amounts of Ag and K. The Jahn–Teller effect and electron–lattice coupling were enhanced with the MnO 6 octahedron undergoes distortion. Moreover, the double-exchange interaction was improved with an increase in the number of Mn 4+ /Mn 3+ ion pairs. Under the influence of these factors, the electrical transport properties especially the temperature coefficient of resistivity ( TCR ) of the LAKMO films can be refined modulated or optimized via A-site Ag and K co-doping. When x = 0.17, the maximum TCR of LAKMO reached 11.23 % K −1 at 293.23 K. Thus, La 0.7 Ag 0.13 K 0.17 MnO 3 film with a large TCR exhibited excellent thermal properties at room temperature and was considered material for application to uncooled infrared bolometers.
研究领域:
材料科学
·
电子工程
✓ 已验证
产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Zihao Chen,Xiaolu Liang,Zimeng Pan,Zhenyu Wang,Yao Wang,Haitao Wang,Jiachen Wang,Jiankun Sun,Jun Ma,Xuexing Jiang,Jubo Peng,Xin Gu,Jing Zhang,Xiang Liu
In this study, La 0.67− x Nd x Ca 0.25 Sr 0.08 MnO 3 (LNCSMO, x = 0.09, 0.10, 0.11, 0.12) films were prepared by sol-gel spin coating method. The results indicate that the LNCSMO film is oriented and grown on a La 0.3 Sr 0.7 Al 0.65 Ta 0.35 O 3 (LSATO) (00 l ) substrate. In addition, with the increase of Nd 3+ content, the Mn-O bond length ( d Mn-O ) and Mn-O-Mn bond angle ( θ Mn-O-Mn ) decreases. The concentration ratio of Mn 4+ increases firstly and then decreases, and resulting in an enhanced and then weakened double exchange (DE) effect. Measuring the variation of sample resistivity with temperature ( ρ -T) using the standard four-probe method. As the content of Nd 3+ increases, the metal-insulator transition temperature ( T p ) gradually decreases, and the resistivity ( ρ ) gradually increases. The replacement of Nd 3+ makes the peak temperature coefficient of resistivity value ( TCR max ) of the material firstly increase and then decrease. At x = 0.11, the TCR max reaches 34.28 % K −1 at 254.72 K. This provides new insights into the impact of Nd doping on electrical transport properties.
研究领域:
材料科学
·
凝聚态物理
✓ 已验证
产品关联
DOI
高影响因子
Signal Transduction and Targeted Therapy
·
2024
·Vol. 9
·期 1
·1-18
·
IF 40.8
Yang Chuan,Wang Xueping,To Kenneth K. W.,Cui Caimei,Luo Min,Wu Shaocong,Huang Lamei,Fu Kai,Pan Can,Liu Zeyu,Fan Teng,Yang Caibo,Wang Fang,Fu Liwu
Circulating tumor cells (CTCs) are precursors of distant metastasis in a subset of cancer patients. A better understanding of CTCs heterogeneity and how these CTCs survive during hematogenous dissemination could lay the foundation for therapeutic prevention of cancer metastasis. It remains elusive how CTCs evade immune surveillance and elimination by immune cells. In this study, we unequivocally identified a subpopulation of CTCs shielded with extracellular vesicle (EVs)-derived CD45 (termed as CD45 + CTCs) that resisted T cell attack. A higher percentage of CD45 + CTCs was found to be closely correlated with higher incidence of metastasis and worse prognosis in cancer patients. Moreover, CD45 + tumor cells orchestrated an immunosuppressive milieu and CD45 + CTCs exhibited remarkably stronger metastatic potential than CD45 − CTCs in vivo. Mechanistically, CD45 expressing on tumor surfaces was shown to form intercellular CD45-CD45 homophilic interactions with CD45 on T cells, thereby preventing CD45 exclusion from TCR-pMHC synapse and leading to diminished TCR signaling transduction and suppressed immune response. Together, these results pointed to an underappreciated capability of EVs-derived CD45-dressed CTCs in immune evasion and metastasis, providing a rationale for targeting EVs-derived CD45 internalization by CTCs to prevent cancer metastasis.
研究领域:
肿瘤学
·
免疫学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
APPLIED PHYSICS LETTERS
·
2022
·Vol. 121
·期 20
·
IF 3.971
Guan Xiaoli,Li Hongjiang,Yu Zhiyuan,Yu Xiaohan,Jin Shuaizhao,Gu Xin,Liu Xiang
A series of La 0.7 K 0.3− x Sr x MnO 3 (0.00 ≤ x ≤ 0.3) films were fabricated on LaAlO 3 substrates using a sol-gel spin-coating technique. The relationship between electrical transport properties and a microstructure was explained via the Jahn–Teller effect, a phenomenological percolation model, and a double-exchange mechanism. The results revealed the strong impact of K and Sr co-occupying A-sites on the formation of Mn 3+ /Mn 4+ pairs, crystal structure, conductivity, surface morphology, and in-plane and out-of-plane strains. The as-fabricated La 0.7 K 0.25 Sr 0.05 MnO 3 films showed a peak temperature coefficient of resistivity of 16.63% K −1 at 298.3 K.
研究领域:
材料科学
·
凝聚态物理
✓ 已验证
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2022
·
IF 5.532
Zhaowei Liang,Sheng'an Yang,Haishan Wang,Yule Li,Junfeng Li,Baokang Hou,Junhong Li,Jie Wang,Liang Wu,Hui Zhang,Qingming Chen,Ji Ma
Perovskite manganites La 0.67 Ca 0.33 MnO 3 (LCMO) holds promising for infrared bolometer applications due to its large temperature coefficient of resistance (TCR) and low 1/ f noise. However, it remains challenging to obtain large TCR value at a wide temperature range, which inhibits its actual application. In this study, we compare the effects of sintering temperature (1200 °C ∼ 1450 °C), vanadium addition (0% and 5%), and vanadium addition route (sol-gel doping and solid-state composite) on the structure, morphology and electrical transport properties of LCMO. We found that the ceramics prepared with higher temperature possess larger grain size and higher density. Vanadium addition considerably increases the grain size as well as improves TCR value. Our findings provide an effective route to promote the infrared detect application of perovskite manganites.
研究领域:
材料科学
·
电气工程
·
红外技术
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Shuaizhao Jin,Xin Gu,Xiaohan Yu,Xiaoli Guan,Yixin Yan,Kaikai Wu,Liming Zhao,Yan Zhu,Shuhong Sun,Jinkun Liu,Jin Hu,Jun Zhao,Lingde Kong,Wenyun Yang,Qingming Chen,Parviz Kameli,Xiang Liu
Compared with conventional thermal and magnetic sensing materials, perovskite manganese oxide films with a rapid shift from ferromagnetic metals to paramagnetic insulators can be more suitable for the fabrication of uncooled infrared bolometers and magnetic sensors. However, the main challenge is to obtain perovskite manganese oxide films with high temperature coefficient of resistivity ( TCR ) and low field magnetoresistance ( LFMR ) at room temperature . In this study, La 1- x K x MnO 3 (LKMO, 0.10 ≤ x ≤ 0.35) films with different K-dopant amounts were prepared on SrTiO 3 substrates using sol–gel spin coating method. A comprehensive analysis of their structure, morphology, and ionic valence enabled one to conclude that varying K-dopant content from 0.10 to 0.30 caused a decrease in Mn-O bond lengths and an increase in Mn-O-Mn bond angles, as well as caused the grain growth and the rise in Mn 4+ content in LKMO films. All these changes resulted in a significant alteration in the resistivity of LKMO films, thereby providing the larger TCR and LFMR values at room temperature. According to the results, La 0.7 K 0.3 MnO 3 with 13.13 % K −1 TCR and La 0.85 K 0.15 MnO 3 with 46.39% LFMR (under an external magnetic field of 1 T) are promising materials for the manufacturing of uncooled infrared bolometers and magnetic sensors, respectively.
研究领域:
材料科学
·
物理学
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2018
·
IF 2.21
Han Zhang,Sixv Zhu,Beibei Guo,Dongyun Wan,Hongjie Luo,Yanfeng Gao
This work first reports the controllable preparation of rGO/VO 2 (B) intercalation films via a simple sol-gel method at relative low temperature (420 °C). The excellent conductance and thermal conductivity of rGO could significantly enhance the performance and sensitivity of VO 2 (B) films to be used for uncooled infrared detectors . Aluminum (Al) reduction is introduced to reduce the oxygen-containing functional groups of graphene oxide (GO) and V 2 O 5 of GO/V 2 O 5 intercalation films in a two-zone furnace. According to our results, the reduced-GO (rGO), as a fast channel for electron transport , was inserted into the VO 2 (B) film, and thus the electron transfer between rGO/VO 2 (B) intercalation films occurs much faster than in pure VO 2 (B) films. The film preparation processes were optimized through adjusting the doping concentration of rGO, and rGO/VO 2 (B) intercalation films with applicable temperature coefficient of resistance (TCR) (−2.78%/K) and square-resistance (21.22 kΩ) were achieved. Our work provided a promising and feasible technique to convict the cost and complexity of high vacuum method used to fabricate the VO 2 thermal sensing thin films .
研究领域:
材料科学
·
纳米技术
·
热感应
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）