PLANT PHYSIOLOGY AND BIOCHEMISTRY
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2020
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IF 3.72
Guanhua Liu,Mei Yang,Jianyu Fu
Terpenes and their derivatives are vital components of tea aroma. Their constitution and quantity are highly important criteria for the sensory evaluation of teas. Biologically, terpenes are involved in chemical resistance of tea plant against biotic and/or abiotic stresses . The goal of this study is to identify volatile terpenes of tea plants implicated in defense against herbivores and to identify terpene synthase ( TPS ) genes for their biosynthesis . Upon herbivory by tea geometrid ( Ectropis obliqua Prout), tea plants were found to emit two sesquiterpenes, ( E , E )-α-farnesene and ( E )-nerolidol, which were undetectable in intact tea plants. The induced emission of ( E , E )-α-farnesene and ( E )-nerolidol suggests that they function in either direct or indirect defense of tea plants against the tea geometrid. Candidate TPS genes were identified from the transcriptomes of tea plants infested by tea geometrids. Two dedicated sesquiterpene synthases, Cs AFR and Cs NES2, were identified. Cs AFR belongs to the TPS-b clade and can catalyze the formation of ( E , E )-α-farnesene from ( E , E )-FPP. Cs NES2 belongs to the TPS-g clade and can synthesize ( E )-nerolidol using ( E , E )-FPP. The two genes were also both dramatically upregulated by herbivore damage. In summary, we showed that two novel sesquiterpene synthase genes CsAFR and CsNSE2 are inducible by herbivory and responsible for the elevated emission of herbivore-induced ( E , E )-α-farnesene and ( E )-nerolidol, which are implicated in tea plant defense against herbivores.
研究领域:
植物科学
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分子生物学
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农学
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昆虫学
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DOI
PubMed
高影响因子
PLANT AND SOIL
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2014
·Vol. 382
·期 1
·103-115
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IF 3.235
Duan Dechao,Peng Cheng,Xu Chen,Yu Mingge,Sun Lijuan,Worden Natasha,Shi Jiyan,Hu Tiandou
Background and aims Metal species in rhizosphere soil profoundly influence their mobility and phytoavailability. Clarifying the speciation transformation of heavy metals helps understand their translocation and accumulation in plants. Methods Single extraction, selective sequential extraction (SSE) and X-ray absorption near-edge structure (XANES) spectroscopy were employed to investigate the speciation transformation of lead (Pb) and its influence on metal accumulation in tea plants after the addition of tea polyphenols (TPs). Results Pb content was decreased in young leaves and stems, whereas increased in roots, after TPs were amended to soil. Both SSE and XANES analysis suggested bioavailable Pb was transformed to organically bound Pb after the addition of TPs. The increased percentage of organically bound Pb might be fixed in the cell wall of plant root through a ternary complex formed between the Pb-organic matter complex and cell wall components. Therefore, Pb translocation from roots to young tissues was decreased. Conclusions Pb phytoavailability change was driven by its speciation transformation after the addition of TPs. Combined SSE and XANES spectroscopy represent powerful tools to study metal speciation transformation in plant and soil systems.
研究领域:
环境科学
·
植物学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Qicai Zhang,Xianli Wang,Chengbin Liu,Huaxi Li,Zhongsheng Deng,Chunxia Yao,Yameng Li,Qinxiong Rao,Weiguo Song
Given the high toxicity of N-(1,3-dimethylbutyl)-N′-phenyl- p -phenylenediamine (6PPD) derivatives, such as 6PPD quinone (6PPDQ) to salmon, as well as their ubiquitous presence in the environment, the contaminant of aquatic food products has drawn significant attention. However, analytical methods for p -Phenylenediamines (PPDs) and their transformation products (TPs) in aquatic products remain underdeveloped. In particular, the degradation of some compounds and strong matrix effects complicate detection. In this study, we present a stable, rapid, and sensitive method combining salt-out assisted extraction, antioxidant protection, and multi-plug filtration clean-up (m-PFC) to detect two PPDs and five TPs in aquatic products. Crucially, the appropriate selection of antioxidants prevented the degradation of the easily oxidized target compounds. Further, the m-PFC method significantly enhanced the purification efficiency, achieving satisfactory recoveries (62.1–115 %), and method detection limits (MDLs) ranging from 0.00300 to 0.400 μg/kg. Subsequently, the method was applied to monitor PPDs and their TPs in aquatic products systematically, revealing the presence of 6PPD and N-isopropyl-N′-phenyl-1,4-phenylenediamine (IPPD) in white shrimp from aquafarms, whereas none of the seven target analytes were detected in fish and crab samples. These findings contribute to the detection of PPDs, their TPs and other unstable chemicals in aquatic products, thereby providing insights into their concentrations in these products.
研究领域:
分析化学
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环境科学
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食品安全
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产品关联
DOI
PubMed
高影响因子
POLYMER
·
2026
·
IF 4.5
Long Chen,Hongli Jin,Jiakang Zhang,Xinjie Zhang,Zhen He,Tao Fu,Yuhui Zhang,Yanchun Zheng,Meidong Lang
In order to address the poor compatibility between thermoplastic starch (TPS) and polyester during the blending process, this study employed the melt blending method to prepare poly (ethylene adipate- co -terephthalate) (PEAT)/thermoplastic starch (TPS) composite films. A series of composite films were evaluated for mechanical properties, micromorphology, thermal behavior, water absorption, and water vapor and oxygen barrier, systematic study was conducted on the effect of the amount of reactive chain extender poly((phenyl isocyanate)- co -formaldehyde) (PAPI) addition for the performance of PEAT/TPS/PAPI composite films. The results indicate that PAPI, as the reactive compatibilizer, effectively improves the interfacial adhesion of PEAT/TPS blends. The research findings indicate that PAPI enhances the compatibility between PEAT and TPS by forming urethane bonds. The incorporation of PAPI significantly enhances the mechanical properties of the composite film, with tensile strength and tensile modulus increasing by 62 % and 88 % respectively, and toughness increasing by 48 %. Moreover, the incorporation of PAPI has enhanced the water vapor barrier properties of the composite film by 44 %, and improved its oxygen barrier properties by 26 %. The results obtained in this study provide a reference for the manufacture of PEAT/TPS composites, which will facilitate the practical application of PEAT/TPS composite films in the packaging film.
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产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Fengqing Chen,Yan Feng,Feixiang Yin,Teng Huang,Qiao Kang,Dan Xie,Junhui Zhu,Yiping Wen,Baiyu Jiang,Jinfeng Dai
Multifunctional value-added biodegradable materials are significant for addressing issues of plastics waste in a green, cost-effective, and energy-saving way. In this work, the nano mica (Mica) was both exfoliated and interfacial double esterified by critic acid (CA) in a facile one-step method to achieve multifunctional biodegradable polybutylene adipate/terephthalate (PBAT)/thermoplastic starch (TPS) films. As a result, the obtained composite with 1 phr Mica (P4T6-1Mica) demonstrated excellent comprehensive performances under the coordination of esterification and acidolysis of CA. The crystallinity and tensile strength of P4T6-1Mica were 240 and 56% higher than those of original composite (P4T6C0.5), attributed to the heterogeneous nucleation of Mica and improved interfacial compatibility. P4T6-1Mica also exhibited a lower water vapor transmission rate (WVT) of 73.39 g/m2·24 h, representing a 39.3% improvement over P4T6C0.5. The decreased WVT was assigned to the hindering paths caused by CA esterification and acidolysis and the original barrier of Mica. At 10 weeks, the biodegradation rates for P4T6C0.5, P4T6-1Mica, and P4T6-2Mica were 56.3, 37.5, and 33.1%, respectively, indicating regulable biodegradation. The incorporation of exfoliated Mica even enhances UV resistance and transmittance of the film. The design of double esterification and a one-step process offers sustainable, affordable, and energy-saving approaches for developing multifunctional biodegradable materials to address plastic pollutions.
研究领域:
材料科学
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高分子化学
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生物降解材料
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纳米复合材料
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环境工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Yuan Zhang,Félix Manuel Rosado-García,Yamila Puig Peña,Panagiotis Karanis,Xin Yu,Mingbao Feng,Chengsong Ye
Human beings release thousands of antibiotics into the environment, which could generate the related transformation products (TPs), most of which have yet to be identified and lack rigorous microbial risk information. This study aimed to investigate the impact and mechanisms of 4-nitro sulfamethoxazole, N 4 -acetylated sulfamethoxazole, and N 4 -acetylated sulfadiazine, three typical sulfonamide (SAs) metabolites, on the risk of antibiotic resistance genes (ARGs) transmission. The results revealed that TPs significantly enhance the risk of conjugative transfer of RP4 plasmid at clinically and environmentally relevant concentrations (10 ng/L to 100 μg/L), with a maximum increase of up to 73-fold. These three metabolites’ capabilities to enhance the conjugative transfer of ARGs are more pronounced than the parent sulfonamides. The induction mechanisms of TPs on ARGs transmission are also more complex, which primarily arise from the enhancement of reactive oxygen species, further increased cell membrane permeability and upregulated bacterial secretion systems. Transcriptomic analysis validated the aforementioned biological processes and showed that TPs also increased the activity of toxin-antitoxin system and bacterial intracellular transposon, thereby promoting the spread of ARGs. This research contributes to a better understanding of the antibiotic-like effects of TPs, which is crucial for improving our understanding of non-antibiotic drug-induced bacterial resistance risks.
研究领域:
环境科学
·
微生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2024
·
IF 3.7
Liwen Wang,Ling Xin Yong,Say Chye Joachim Loo
This is the first study that explores blending polylactic acid (PLA) with various biomasses, including food wastes─brewer’s spent grain (BSG), spent coffee grounds (SCG), sesame cake (SC), and thermoplastic starch (TPS) biomass to create composite gastric floating drug delivery systems (GFDDS) through 3D printing. The aim is to investigate the influence of biomass percentage, biomass type, and printing parameters on their corresponding drug release profiles. 3D-printed (3DP) composite filaments were prepared by blending biomasses and PLA before in vitro drug release studies were performed using hydrophilic and hydrophobic model drugs, metoprolol tartrate (MT), and risperidone (RIS). The data revealed that release profiles were influenced by composite compositions and wall thicknesses of 3DP GFDDS capsules. Up to 15% of food waste could be blended with PLA for all food waste types tested. Delivery studies for PLA-food wastes found that MT was fully released by 4 h, exhibiting burst release profiles after a lag time of 0.5 to 1.5 h, and RIS could achieve a sustained release profile of approximately 48 h. PLA-TPS was utilized as a comparison and demonstrated variable release profiles ranging from 8 to 120 h, depending on the TPS content. The results demonstrated the potential for adjusting drug release profiles by incorporating affordable biomasses into GFDDS. This study presents a promising direction for creating delivery systems that are sustainable, customizable, and cost-effective, utilizing sustainable materials that can also be employed for agricultural, nutraceutical, personal care, and wastewater treatment applications.
研究领域:
材料科学
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药剂学
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可持续工程
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生物医学工程
·
3D打印技术
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DOI
高影响因子
JOURNAL OF MICROENCAPSULATION
·
2016
·
IF 1.631
Xunan Zhang,Wei Zong,Ying Hu,Nan Luo,Wenlong Cheng,Xiaojun Han
In order to increase the loading efficiency of drug carriers, here we demonstrated a microfluidic method to fabricate an asymmetric vesicle, which contains trichloro(1H,1H,2H,2H-perfluoroocty-l)silane (TPS) inner leaflet and lipid outer leaflet. The asymmetric vesicle was characterised by fluorescence microscopy and Fourier transform infra-red spectrum. In vitro cytotoxicity of the vesicles carrying 5-fluorouacil (5-FU) has also been studied.
研究领域:
药学
·
纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Li Jiang,Chengfang Zhang,Xinyue Xu,Rui Hu,Ping Zhang,Rensheng Wang,Xinjian Chen,Liang Hu
Dose verification in radiotherapy is crucial to ensure a safe and efficient outcome of medical treatments. To overcome routine radiation dose detection challenges, a composite hydrogel sensor comprised of fluorescent poly( N -isopropylacrylamide)-based nanogels and clay nanoparticles is reported. The sensing methodology is primarily based on the transition of hydroxyphenyl fluorescein to fluorescence in the presence of OH radicals due to the radiolysis of water molecules under ionizing radiation, whereas the fluorescence intensity of 5(6)-carboxytetramethylrhodamine is stable upon radiation. Harnessing the ratiometric fluorescent strategy, in vitro point- and topographical-dose profiles under X-ray and electron beam irradiation and the in-vivo determination of γ-ray dose are determined without any waiting time after irradiation. The determined dose is comparable to the results of Monto Carlo simulations and clinical treatment planning systems (TPS). Also, the sensing capability of the developed sensor is well maintained, regardless of the types of radiation, dose rate, radiation energy, testing temperature, and storing period. Hence, our sensors show the translational potential for dose verification in radiotherapy.
研究领域:
辐射物理学
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生物医学工程
·
医学物理
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2021
·
IF 3.963
Chao Xu,Hongzhou Chen,Qinyan Wu,Yuqi Wu,Paul Daly,Jian Chen,Hongfu Yang,Lihui Wei,Yiqing Zhuang
Fusarium head blight( FHB )caused by Fusarium graminearum species complex (FGSC) is one of the most important diseases around the world. Deoxynivalenol (DON) is a type of mycotoxin produced by FGSC when infecting cereal crops . It is a serious threat to the health of both humans and livestock. Trehalose-6-phosphate phosphatase ( TPP ), a conserved metabolic enzyme found in many plants and pathogens, catalyzes the formation of trehalose. N-(phenylthio) phthalimide (NPP) has been reported to inhibit the normal growth of nematodes by inhibiting the activity of TPP , but this inhibitor of nematodes has not previously been tested against F. graminearum . In this study, we found that TPP in F. graminearum (Fg TPP ) had similar secondary structures and conserved cysteine (Cys356) to nematodes by means of bioinformatics. At the same time, the sensitivity of F. graminearum strains to NPP was determined. NPP exhibited a better inhibitory effect on conidia germination than mycelial growth . In addition, the effects of NPP on DON biosynthesis and trehalose biosynthesis pathway in PH-1 were also determined. We found that NPP decreased DON production, trehalose content, glucose content and TPP enzyme activity but increased trehalose-6-phosphate content and trehalose-6-phosphate synthase ( TPS ) enzyme activity. Moreover, the expression of TR I 1 , TR I 4 , TR I 5 , TR I 6 , and TPP genes were downregulated, on the contrary, the TPS gene was upregulated. Finally, in order to further determine the control ability of NPP on DON production in the field, we conducted a series of field experiments, and found that NPP could effectively reduce the DON content in wheat grain and had a general control effect on FHB. In conclusion, the research in this study will provide important theoretical basis for controlling FHB caused by F. graminearum and reducing DON production in the field.
研究领域:
植物病理学
·
真菌学
·
农学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）