BMC PLANT BIOLOGY
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2024
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IF 4.3
Minmin Chen,Xiaoqun Wang,Xiawen Zhou,Baiyu Huang,Yujie Zhao,Haiying Liu,Qiuling He
Tetrastigma hemsleyanum , a traditional Chinese medicinal plant with anti-inflammatory, anti-cancer, and anti-tumor properties, faces increasing abiotic stress due to climate change, agricultural chemicals, and industrialization. This study investigated how three abiotic stress factors influence antioxidant enzyme activity, MDA levels, DPPH free radical scavenging capacity, chlorophyll, carotenoids, active compounds, and gene expression in different T. hemsleyanum strains. The comprehensive evaluation indicates that the ZJWZ strain holds potential as a preferred parental material for future resistance breeding. Furthermore, PAL gene expression was strongly positively correlated with flavonoid and phenol contents, highlighting its role in the stress response through the phenylpropanoid-flavonoid pathway. This study contributes to the standardization of the production and breeding of superior strains of T. hemsleyanum . It also lays the foundation for investigating how plants react to environmental stressors.
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高影响因子
Food & Function
·
2021
·Vol. 12
·期 18
·8351-8365
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IF 5.396
Yonglu Li,Xin Yu,Yaxuan Wang,Xiaodong Zheng,Qiang Chu
Lung cancer has been threatening human health worldwide for a long time. However, the clinic therapies remain unsatisfactory. In this study, the anti-adenocarcinoma lung cancer A549 cell line abilities of Tetrastigma hemsleyanum tuber flavonoids (THTF) were evaluated in vivo, and isobaric tags for relative and absolute quantification (iTRAQ)-based proteomic analysis was conducted to detect the protein alterations in THTF-treated solid tumors. The differentially expressed proteins were related to the cytoskeleton and mostly accumulated in the calcium signaling pathway. The in vitro study illustrated that 80 μg mL−1 THTF significantly suppressed cellular viability to approximately 75% of the control. Further results suggested that kaempferol-3-O-rutinoside (K3R), the major component of THTF, effectively triggered cytoskeleton collapse, mitochondrial dysfunction and consequent calcium overload to achieve apoptosis, which remained consistent with proteomic results. This study uncovers a new mechanism for THTF anti-tumor ability, and suggests THTF and K3R as promising anti-cancer agents, providing new ideas and possible strategies for future anti-lung cancer prevention and therapy.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Zhiyan Jiang,Junyao Liu,Caihua Shao,Siying Wang,Juan Xu,Ying Wang,Xiaoyu Lin,Haishun Xu,Xueqian Wu
Flavonoid compounds in Tetrastigma hemsleyanum Diels et Gilg ( T. hemsleyanum ) are essential for plant stress responses, yet their biosynthetic pathways and regulatory mechanisms remain poorly understood. This study identified and characterized two flavanone-3-hydroxylase ( F3H ) genes, Th F3H1 and Th F3H2, from T. hemsleyanum , which belong to the 2ODDs-DOXC28 subfamily. Tissue-specific expression analysis revealed that both genes are highly expressed in tuberous roots, with distinct stress response patterns observed. Sequence and phylogenetic analyses confirmed that these genes contain typical structural domains of the 2ODD family and are closely related to F3H proteins from various plant species. Subcellular localization studies indicated that Th F3H1 is predominantly cytoplasmic, while Th F3H2 is found both in the cytoplasm and the nucleus. In vitro enzymatic assays demonstrated that both enzymes catalyze the conversion of naringenin to dihydrokaempferol and small amounts of apigenin, with Th F3H2 exhibiting higher catalytic efficiency ( k cat / K m = 0.0093 s −1 ·μM −1 ). AlphaFold2 predictions and molecular docking analysis revealed the binding modes of Th F3H1 with itssubstrates. Site-directed mutagenesis further identified four key catalytic residues (Glu122, Lys196, Lys326, and Asp330). Furthermore, both genes were significantly upregulated under salt stress, and their heterologous expression enhanced the salt tolerance of Escherichia coli , underscoring their critical roles in plant stress responses. These findings enhance the understanding of plant F3H function and provide a molecular foundation for improving the quality and stress resistance of T. hemsleyanum .
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BIOMACROMOLECULES
·
2026
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IF 5.9
Jiashuo Ye,Dayang Wang,Ziyan Wang,Hailin Cong,Bing Yu
Lung cancer has the highest global incidence and mortality, with nonsmall cell lung cancer (NSCLC) as the predominant subtype. Traditional therapies are limited by drug resistance, systemic toxicity, and poor precision. Active ingredients from Chinese herbs offer promising alternatives. This work identified kaempferol (KAE) from Tetrastigma hemsleyanum Diels et Gilg as an anti-NSCLC agent and developed a pH/photothermal dual-responsive nanocarrier (KMPB@PL-HA) for targeted therapy. The nanocarrier uses mesoporous Prussian blue (MPB) as the photothermal core, polylysine (PLL), and ultra-active hyaluronic acid (UL-HA) as the pH-responsive shell. In the tumor microenvironment (TME), UL-HA binds to CD44 receptors overexpressed on NSCLC cells and dissociates, while PLL enhances cellular uptake via electrostatic adsorption. KAE induces mitochondrial apoptosis via p-Akt inhibition, Bax/Bcl-2 regulation, and caspase cascade activation, and downregulates VEGF and modulates TME redox homeostasis for synergistic antitumor effects. This study provides a novel chemo-photothermal synergistic strategy for NSCLC.
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Food Bioscience
·
2024
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IF 4.8
Kexin Tao,Han Peng,Xin Bi,Xiaoya Wang,Yue Tian,Hua Zhang,Yong Sun
Alcoholic liver disease (ALD) is caused by chronic heavy alcohol consumption and is characterized by lipid metabolism disorders, oxidative stress , and hypersecretion of proinflammatory cytokines . Tetrastigma hemsleyanum leaves (THL), a popular functional food in China, have good anti-inflammatory, antioxidant, and liver protection effects. However, whether THL extracts improve alcoholic liver disease and the underlying mechanism of action have not been determined. Therefore, in this study, we used a C57BL/6J male mouse model to investigate the effect of THL extract on ALD and to explore the underlying mechanism involved preliminarily. The alleviation of alcohol-induced liver dysfunction and changes in lipid metabolism-related pathways were observed after THL extract administration stimulated fatty acid synthesis and fatty acid β -oxidation by modulating the expression levels of related mRNAs and genes, thereby alleviating lipid accumulation and oxidative stress in the liver. The composition and abundance of the gut microbiota in mice also underwent alterations. Collectively, these findings suggest that supplementation with THL extract can mitigate liver damage and reduce hepatic lipid accumulation in mice with alcoholic fatty liver disease. It regulates hepatic metabolism and generates various metabolites associated with diminished lipid buildup, such as Plafibride, LysoPI (20:4(5Z,8Z,11Z,14Z)/0:0), 1,3,7-trimethyluric acid, and phytol. Moreover, it impacts specific gut microbiota in mice with alcoholic fatty liver disease including Bacteroides and other beneficial microorganisms while enhancing the production of short-chain fatty acids to improve the intestinal microenvironment.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Yinan Qiu,Jia Gao,Wenhui Chu,Shanshan Xia,Chen Huang,Huayue Zhu,Xiaolong Sun,Yongqian Fu
Acute lung injury (ALI) is a critical respiratory syndrome significantly impacting patient health. Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing, SYQ) is a traditional Chinese medicine and its polysaccharides (SYQP) have demonstrated efficacy in counteracting lipopolysaccharide-induced ALI. This study characterized the structure of SYQP and synthesized the SYQP@ZIF-8 composite using biomimetic mineralization, evaluating encapsulation and release efficiency. The biocompatibility of SYQP@ZIF-8 in vitro was assessed by the CCK-8 colorimetric assay and hemolytic activity. Inflammatory cytokine was measured to evaluate the therapeutic effect. The efficacy of SYQP@ZIF-8 in lung injury was assessed using a mice ALI model. Characterization showed SYQP as a homogeneous α-type polysaccharide, comprising galactose, mannose, glucuronide, glucose, galacturonide, and arabinose, with a molecular weight of 516.94 kDa. SYQP@ZIF-8 exhibited high encapsulation rate (> 90 %), rapid pH-responsive release (within 60 min up to ~100 %), low toxicity and favorable hemolytic characteristics. Furthermore, it demonstrated reduced inflammatory cytokine secretion compared to SYQP, along with a superior inhibitory effect. The outcomes of in vivo experiments, including a decrease in the W/D ratio and LDH activity, further confirmed the efficacy of SYQP@ZIF-8 in treating LPS-induced ALI. In conclusion, SYQP@ZIF-8 released SYQP in acidic inflammatory conditions, outperforming SYQP alone in treating ALI.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
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IF 4.784
Qiang Chu,Ruoyi Jia,Wen Chen,Yangyang Liu,Yonglu Li,Xiang Ye,Yong Jiang,Xiaodong Zheng
Ethyl Carbamate (EC), as a carcinogen widely found in fermented foods, was verified that its cytotoxicity was associated with oxidative stress. Polysaccharides from natural sources due to their antioxidative capacity have attracted great attention in the past time. In this study, purified polysaccharide from Tetrastigma hemsleyanum vines (TVP) with 64.89 kDA was extracted and conducted multiple analysis to identify its structural information. It could be discovered that TVP was composed of mannose, rhamnose, glucuronic acid, glucose, galactose, and arabinose. In vitro , TVP could inhibit cytotoxicity and genotoxicity, attenuate oxidative damage and mitochondrial dysfunction induced by EC in Caco-2 cells. Meanwhile, TVP could suppress apoptosis by mTOR and Bcl-2 signaling pathways, ameliorate oxidative via Sirt1-FoxO1 and Nrf2-Keap1 signaling pathways. In vivo , EC as well triggered the decline of survival and athletic ability in Caenorhabditis elegans ( C. elegans ) and TVP could reverse the decline. In the meantime, TVP could ameliorate oxidative damage in N2 and daf-2 (-) mutant but fail in daf-16 (-) mutant, which suggested that DAF-16 (FOXO) might affect the antioxidative protection of TVP in C. elegans . In brief, our results manifested that TVP could attenuate EC-induced cytotoxicity both in vitro and in vivo .
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CARBOHYDRATE POLYMERS
·
2025
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IF 12.5
Shasha Tian,Zian Mao,Yuexia Wang,Kewei Li,Yafei Li,Bingqi Zhu,Fangmei Zhou,Jingchao Li,Yingzhi Shen,Zhishan Ding
Tetrastigma hemsleyanum Diels et Gilg ( T. hemsleyanum ) is a rare traditional Chinese medicine with high medicinal value and T. hemsleyanum polysaccharides (THPs) have attracted increasing attention in recent years. However, detailed structural and functional insights into THPs remain limited. Here, a novel pectic polysaccharide (STHP4) with an average molecular weight (Mw) of 119 kDa was extracted and purified from the aboveground part of T. hemsleyanum . Structurally, STHP4 was mainly composed of galacturonic acid, glucuronic acid, and galactose, with minor glucose, rhamnose, arabinose, and mannose, and its primary backbone was 1,4-GalA. Pectinase treatment of STHP4 yields two distinct fractions: STHP4-1 and STHP4-2. STHP4-1 shared the same types of monosaccharides with STHP4, except for the absence of glucose, but had a broad Mw distribution (9.9 to 251 kDa). Another product, STHP4-2, a mixture of oligosaccharides and free monosaccharides, has a Mw of 1.387 kDa. Their structures were further elucidated via methylation analysis and NMR spectra. Furthermore, STHP4-1 exhibited superior immune regulatory effects on macrophages, including increased nitric oxide release and elevated interleukin-6, interleukin-1β, and tumor necrosis factor α expression. These findings suggest that STHP4-1 might be the core active domain of STHP4, and both STHP4 and STHP4-1 are potential immune enhancers.
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高影响因子
Foods
·
2021
·Vol. 10
·期 9
·2123
·
IF 4.35
Yong Sun,Fanghua Guo,Xin Peng,Kejun Cheng,Lu Xiao,Hua Zhang,Hongyan Li,Li Jiang,Zeyuan Deng
Tetrastigma hemsleyanumDiels et Gilg is a herbaceous perennial species distributed mainly in southern China. TheTetrastigma hemsleyanumroot (THR) has been prevalently consumed as a functional tea or dietary supplement. In vitro digestion models, including colonic fermentation, were built to evaluate the release and stability of THR phenolics with the method of HPLC-QqQ-MS/MS and UPLC-Qtof-MS/MS. From the oral cavity, the contents of total phenolic and flavonoid began to degrade. Quercetin-3-rutinoside, quercetin-3-glucoside, kaempferol-3-rutinoside, and kaempferol-3-glucoside were metabolized as major components and they were absorbed in the form of glycosides for hepatic metabolism. On the other hand, the total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, and glutathione (GSH) content were significantly increased, while malondialdehyde (MDA) content was decreased in plasma and tissues of rats treated with THR extract in the oxidative stress model. These results indicated that the THR extract is a good antioxidant substance and has good bioavailability, which can effectively prevent some chronic diseases caused by oxidative stress. It also provides a basis for the effectiveness of THR as a traditional functional food.Keywords:Radix Tetrastigma;colonic fermentation;phenolics;antioxidant capacity;bioavailability
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Zili Guo,Lisha Chen,Xianrui Liang
Tetrastigma hemsleyanum Diels et Gilg (TH) is one of the new eight Genuine Medicinal Materials of Zhejiang. It has extensive biological activities , such as anti-inflammatory, anti-tumor and analgesic activities, etc. In this study, the chemical components of TH were systematically investigated by ultra high-performance liquid chromatography-tandem quadrupole time of flight mass spectrometry (UPLC/Q-TOF-MS). Based on the MS spectrum, 39 compounds in TH extracts including 14 flavonoids , 10 fatty acids, 5 polyphenols and phenolic acids, 4 terpenes and other compounds were detected and tentatively identified. TH samples were treated under different drying methods (vacuum freeze drying , hot air drying, natural drying, light drying and vacuum drying). Besides, the effect of different drying methods on the content of 10 main chemical constituents in TH extracts including catechin , rutin , kaempferol-3- O -rutinoside and so on was also investigated by targeting metabolomics method with ultra high-performance liquid chromatography-tandem triple quadrupole mass spectrometry (UPLC-MS/MS) assisted by multivariate statistical analysis. Large differences were observed between vacuum drying and vacuum freeze drying with remarkable content changes. The contents of rutin, proanthocyanidin B1 and catechin were the most different among the various drying methods. The systematic identification of chemical constituents is helpful for the further medicinal development and application of TH. The effects of drying methods on the content of TH components were studied, which provided experimental data for the processing, storage and quality control of TH.
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