ENVIRONMENT INTERNATIONAL
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2022
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IF 13.352
Peng Zhang,Guanghua Lu,Yu Sun,Jiaqi Zhang,Jianchao Liu,Zhenhua Yan
Size effects of microplastics have received extensive attention for their influence on other pollutants and harm to organisms. In this study, we investigated the uptake, elimination, tissue distribution and potential toxicity mechanism of roxithromycin (ROX) in the presence of 0.5, 5 and 50 μm of aged microplastics (AMPs) in Carassius auratus . The results showed that AMPs promoted the ROX bioaccumulation of various tissues in a size-dependent manner. AMPs and ROX significantly induced superoxide dismutase and catalase activities of liver and gut, and inhibited acetylcholinesterase activities of brain. The coexistence of smaller AMPs exacerbated pathological abnormalities in liver, gill and brain induced by ROX, while larger AMPs caused more intestinal damage. Moreover, high-throughput 16S rRNA gene sequencing indicated that the abundance of Proteobacteria in 0.5 μm AMPs and ROX joint treatments and Firmicutes and Bacteroidota in 50 μm AMPs and ROX joint treatments were significantly raised ( p < 0.05). Metabolomics revealed that AMPs and ROX had a size-dependent long-term effect on gut microbial metabolites, which was mainly related to galactose metabolism, amino acid metabolism and primary bile acid biosynthesis pathways after a 7-day elimination, respectively. These results provide important insights into the relationship between the size effect of AMPs and interaction mechanism of AMPs and coexisting pollutants on aquatic organisms.
研究领域:
环境科学
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毒理学
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水生生态学
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微生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2025
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IF 4.9
Limo Wang,Danfeng Jian,Bingjie Xu,Zhiye Qiu,Yujie Gao,Jindan Wu
The vicious cycle of reactive oxygen species (ROS) overload and bacterial infection critically impedes diabetic wound healing. While nanozyme-antibiotic dressings offer therapeutic potential, their uncontrolled agent release remains a key challenge. In this study, a functional nanofiber membrane (EIPR) based on the pH-sensitive polymer Eudragit S100 was fabricated via coaxial electrospinning to load iridium nanozymes (Ir NPs-PVP) and roxithromycin (Rox), enabling on-demand release of therapeutic agents for combined antibacterial and antioxidant wound therapy. The obtained dressing displays highly precise and controllable therapeutic agent release characteristics. Rox shows approximately 90 % cumulative release at pH 7.4, while release is suppressed to <8 % at pH 6.0 and 4.5. Notably, the release trend of Ir NPs-PVP mirrors that of Rox, with significantly higher release under alkaline conditions. The results exhibit >97 % eradication of both Staphylococcus aureus ( S. aureus ) and Escherichia coli ( E. coli ) coupled with >75 % ROS clearance at pH 7.4. This study presents a novel dual-function system, highlighting the simplicity and efficiency of electrospinning technology, which are key advantages enhancing its scalability for large-scale manufacturing and suitability for clinical wound care translation.
研究领域:
生物医学工程
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伤口护理
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纳米医学
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抗菌材料
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药物递送系统
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产品关联
DOI
高影响因子
FISH & SHELLFISH IMMUNOLOGY
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2023
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IF 4.7
Donghao Yuan,Shidong Wang,Xue Li,Muzi Zhang,Ming Li
As an inevitable factor in aquaculture, ammonia plays a critical role in macrolide antibiotic resistance, leading to accumulating of antibiotic-resistant bacteria in fish skin mucus. In this study, four experimental groups were implemented to test the effects of ammonia alone or in combination with roxithromycin for 28 days on skin mucus microbial composition and the immune response of yellow catfish: CON (control), AN (50.00 mg L −1 total ammonia nitrogen, TA-N), ROX (100 μg L −1 roxithromycin), and HR (50.00 mg L −1 TA-N, 100 μg L −1 ROX). This study demonstrated that ammonia or roxithromycin exposure resulted in increased plasma ammonia content and decreased total antioxidant capacity. Compared with AN group, the combined exposure of ammonia and roxithromycin inhibited the skin mucus immune response. Microbial composition analysis showed that combined exposure of ammonia and roxithromycin had no significant effect on skin mucus α-diversity as compared with CON group. The abundance of Cetobacterium , Rhizobiales_Incertae_Sedis_uncultured and Acinetobacter was increased significantly with the combined effect of ammonia and roxithromycin, these bacteria may be potentially antibiotic-resistant. As compared with CON group, the combined exposure of ammonia and roxithromycin did not affect skin goblet cell counts. This study suggests that combined exposure to ammonia and ROX increases the risk of the emergence of antibiotic-resistant bacteria.
研究领域:
水产养殖
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微生物学
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免疫学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2021
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IF 10.588
Peng Zhang,Guanghua Lu,Yu Sun,Zhenhua Yan,Tianjian Dang,Jianchao Liu
The aging process changes the physicochemical structure of microplastics and affects environmental behaviors and toxicological effects of coexisting pollutants, thereby posing ecological risks. In this study, the effects of aged polystyrene microplastics alone or in combination with the 100 μg/L roxithromycin (ROX) on intestines of Carassius auratus were investigated. The carrier effect of microplastics was enhanced by aging due to changes in functional groups and surface area, which led to an increase in the bioaccumulation of ROX. The combined exposure of aged microplastics (APS) and ROX caused more inflammatory cell infiltration and cilia defects, and significantly inhibited the activity of amylase and lipase. Metagenomic sequencing revealed that the combined exposure of microplastics and ROX increased the abundance of Gemmobacter , Bosea , Rhizobium , and Shinella and decreased the abundance of Cetobacterium and Akkermansia ( p < 0.05). The presence of APS enhanced the selective enrichment of antibiotic resistance genes. What's more, the influence of microplastics and antibiotics on gut microbiota was closely related to carbohydrate metabolism and amino acid metabolism activities, as well as the abundance of baca and sul1 resistance genes. These results expand our understanding of the interaction mechanism between APS and antibiotics in real aquatic environment.
研究领域:
环境科学
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微生物学
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毒理学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2019
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IF 4.213
Jianfu Bai,Han Wang,Wei Gao,Feng Liang,Zixu Wang,Ying Zhou,Xingzi Lan,Xun Chen,Nian Cai,Weimin Huang,Yadong Tang
Implanted scaffold or bone substitute is a common method to treat bone defects. However, the possible bone infection caused by orthopaedic surgery has created a challenging clinical problem and generally invalidate bone repair and regeneration. In this study, a poly (ε-caprolactone) (PCL)/polyethylene glycol (PEG)/roxithromycin (ROX) composite scaffold was prepared via melt electrohydrodynamic (EHD) 3D printing. Fourier transform infrared spectroscopy (FTIR) spectroscopy was performed to verify the existence of PEG and ROX in the scaffolds. By water contact angle measurement, the addition of both PEG and ROX was found to improve the hydrophilicity of the scaffolds. By in vitro drug release assay, the PCL/PEG/ROX scaffolds showed an initial burst drug release and subsequent long-term sustained release behaviour, which is favourable for the prevention and treatment of bone infections. The antibacterial assays against E. coli and S. aureus demonstrated that the composite scaffold with ROX possessed effective antibacterial activity, especially for S. aureus , the main cause of bone infection. The immunostaining and MTT assay with human osteoblast-like cells (MG63) indicated that cells showed good viability and growth on the scaffolds. Therefore, the melt EHD 3D printed PCL/PEG/ROX scaffold could be a promising anti-infective implant for bone tissue engineering.
研究领域:
生物医学工程
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组织工程
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药剂学
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材料科学
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骨科
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2026
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IF 7.3
Runzi Zhang,Shunbi Xie,Xiaoyu Yang,Wanyi Xiong,Yi He
Currently, surface-enhanced Raman scattering (SERS) biosensors face practical challenges, including SERS substrate waste and unsatisfactory detection results. Accordingly, the highly efficient and regenerable SERS substrate HOF@Au was collaborated with a target-triggered identifying-cleaving-rolling-assembly cycling nanodevice (ICRACN) as a cascade signal amplification system to achieve an ultrasensitive detection of roxithromycin (RXM). In this system, abundant Au nanoparticles (NPs) were reduced in situ on the surface of alkali-resistant HOF-102 to form a uniform and high-enhancement SERS substrate HOF@Au, thereby enhancing the accuracy and stability of the sensor. Furthermore, aptamer-mediated specific recognition, efficient cleavage activity of DNA nanorollers, and a pH-responsive DNA triplex structure were engineered to construct ICRACN. In the presence of the target, it was specifically recognized by the aptamer, leading to the activation of the DNAzyme. Meanwhile, the dynamic nanorollers were triggered to initiate cleavage of a specific site along a defined pathway, enabling highly efficient signal amplification. Concurrently, the triplex structure formed by the two single-stranded DNA atoms on the SERS substrate was opened, allowing the capture of the SERS tag and generating a pronounced SERS signal. The proposed strategy achieved the sensitive detection of RXM with a low limit of detection of 3.97 × 10–14 mol/L. Moreover, modulation of pH induced reversible folding and unfolding of the triplex structure to facilitate regeneration of the SERS substrate, which simplified operational procedures and reduced detection costs. As a result, this work presents a novel, regenerable SERS biosensor and offers valuable insights into the design of multifunctional sensing platforms for analytical applications.
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Yi He,Xiaoyu Yang,Shunbi Xie,Runzi Zhang,Yao Liu,Mengjun Wang
Roxithromycin (ROX) is an effective antibiotic, but its prolonged administration may lead to hepatotoxicity and other side effects. To address this concern, the present study introduced a novel strategy that integrated a CHA-mediated dual-end DNA walker with hydrogen-bonded organic frameworks (HOFs) combined with Au nanoparticles (NPs) as efficient SERS substrates, enabling highly sensitive detection of ROX in food. Notably, HOF possessed a large specific surface area and strong adsorption properties that making it excellent platforms for the in-situ synthesis of abundant Au NPs and contributed to robust and uniform SERS signals. When ROX was present, it can trigger the activation of CHA and resulting the formation of a dual-end DNAzyme structure. Meanwhile, under the catalytic action of Mg 2+ , DNAzyme initiated the DNA walker mechanism and generated a substantial amount of Output DNA. Moreover, the Au@4-NTP@Ag core-shell structure generated numerous SERS “hot spots” and combined with the signal-amplifying capability of HOF@Au, leading to a remarkable improvement in SERS sensitivity. Based on this mechanism, the developed sensor employs a “signal-on” strategy for ultra-sensitive detection of ROX, achieving a detection range from 1.0 × 10 −6 to 1.0 × 10 −13 mol/L with a detection limit of 3.72 × 10 −14 mol/L. Furthermore, the method had been successfully applied to the detection of ROX in real food samples, including milk and honey, offering a promising and innovative framework for the development of next-generation SERS biosensors. This approach demonstrated significant potential for highly selective and sensitive antibiotic monitoring in food safety applications.
研究领域:
分析化学
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生物传感器
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纳米技术
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食品安全
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抗生素检测
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
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2022
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IF 11.889
Lei Cao,Yuanling Li,Peining Li,Xueting Zhang,Lei Ni,Li Qi,Haitao Wen,Xinbo Zhang,Yufeng Zhang
The present study operated the novel moving bed biofilm reactor-nanofiltration-membrane bioreactor (MBBR-NF-MBR) with loose polyamide NF membranes for the first time to treat roxithromycin (ROX) wastewater. Results showed that both MBBR-NF-MBRs achieved superior COD removal of 98.4% and 97.2% and excellent removal of ROX at 74.1% and 65.5%, respectively. The main membrane fouling mechanism was reversible fouling caused by the combination of abundant polysaccharides, proteins and Ca-P precipitates, which could be effectively removed by acidic cleaning. Sorption and biodegradation were the main removal routes of ROX in MBBR . Partial retention of loose NF membrane contributed to microbial metabolism and increased microbial diversity, especially the genera Hyphomicrobium in attached biofilm, which was reasonable for ROX removal. The cleavage of cladinose, demethylation, phosphorylation and β-oxidation in macrolactone ring were the main biotransformation reactions of ROX. This study provides novel insights for micropollutants wastewater treatment by using loose NF membrane in MBR.
研究领域:
环境工程
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废水处理
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微生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）