ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Bingzhi Li,Yue Chen,Jing Wang,Qiaoyun Lu,Wanying Zhu,Jieping Luo,Junli Hong,Xuemin Zhou
Sensitive and efficient detection of protein markers, such as transcription factors (TFs), is an important issue in postgenomic era. In this paper, we report a DNA nanodevice, allosteric DNA-silver nanocluster switches (AgSwitches), for TFs detection. The mechanism of this nanodevice is based on the binding-induced allostery whereby the binding between AgSwitches and TFs alters the conformation of AgSwitches. This alteration brings DNA-silver nanocluster (DNA-AgNCs) and guanine-rich enhancer sequences (GRS) into close proximity, generating fluorescent enhancement for quantifications. Our results revealed that the sequence design of AgSwitches can be rationally optimized according to stimulated free energy , and we demonstrated that this method can not only be used for detecting TFs in nuclear extracts of cells, but also be developed as a tool for screening inhibitors of TFs. Overall, this work expanded the category allosteric DNA nanodevices by first introducing DNA-AgNCs into this area, and the obtained method was efficient for TFs-related investigations.
研究领域:
分子生物学
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纳米生物技术
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分析化学
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 8
·1-7
·
IF 6.232
Chen Nandi,Li Junyu,Feng Xianzhen,Yang Yanping,Zhu Li,Chen Xiaomeng,Liu Xuan,Li Yang,Wang Cunchuan,Xia Ligang
A label-free fluorescence method based on self-assembled DNA nanopompom has been developed for miRNA-21 detection. In the presence of miRNA-21, three DNA hairpin probes with split G-quadruplex assemble the DNA nanopompom. Based on the isothermal toehold-mediated DNA strand displacement reaction, the target miRNA can be catalytically recycled and trigger three DNA hairpin probes to self-assemble the DNA nanopompom and release the G-quadruplex. The formation of the G-quadruplex increases the fluorescence emission intensity of thioflavin. For thioflavin-based miRNA-21 detection, the excitation and emission wavelengths are set to 425 nm and 490 nm, respectively. The limit of detection for miRNA-21 is 0.8 pM according to F / F 0 = 0.0031 × C miRNA-21 + 1.0382 ( R 2 = 0.9978). This sensing system provides a low-cost, effective, and convenient method for miRNA detection, which holds great potential in biochemical diagnosis and clinical practice.
Label-free and self-assembled fluorescent DNA nanopompom for miRNA detection
研究领域:
生物化学
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纳米技术
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分子生物学
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分析化学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2022
·
IF 6.51
Yanjuan Huang,Xiuling Dai,Zilin Guan,Di Liu,Lingling Ren,Meixu Chen,Zishan Zeng,Jingwen Jiang,Yong Luo,Yuanfeng He,Min Huang,Chunshun Zhao
Due to a powerful DNA damage repair system and a lack of surface markers, there is currently no effective chemotherapy or tailored targeted therapies available for triple-negative breast cancer (TNBC) treatment. Herein, a tailored DNA damage nanoamplifier ( [email protected] /Pt(IV) C18 ) was engineered to simultaneously induce DNA damage and inhibit DNA reparation for highly efficient TNBC treatment. A newly synthesized Pt(IV) C18 prodrug , the DNA damaging inducer, and the hydrophobic poly(ADP-ribose) polymerases (PARPs) inhibitor niraparib, which is used as the DNA repair blocker, were concurrently encapsulated in highly biocompatible PEGylated liposomes to prepare [email protected] /Pt(IV) C18 , for enhanced cancer therapy and future clinical translation. [email protected] /Pt(IV) C18 with an appropriate size and excellent stability, effectively accumulated at the tumor site. After internalization by tumor cells, niraparib, a highly-selective hydrophobic PARP1 inhibitor, could exacerbate the accumulation of platinum-induced DNA lesions to induce excessive genome damage for synergistic cell apoptosis, which was evidenced by the upregulated γ-H 2 AX and cleaved-PARP levels. Importantly, [email protected] /Pt(IV) C18 exhibited remarkable antitumor efficacy on TNBC without BRCA mutants in vivo with little systemic toxicity. Inspired by the concept of “synthetic lethality”, this study provides an inspirational and clinically transformable nanobased DNA damaging amplification strategy for the expansion of TNBC beneficiaries and highly efficient TNBC treatment via DNA damage induction and DNA repair blocking.
研究领域:
肿瘤学
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纳米技术
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药理学
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癌症治疗
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yuan Yuan,Mengqin Zhang,Guoyang Xie,Jianxiu Wang,Fan Wu,Xinyao Yi
The fabrication of DNA-based nanomaterials with specific functionalities is crucial for advancing tumor therapy. Herein, a novel and facile approach was presented to construct a corona-shaped DNA hybrid nanoplatform, which involved the anchoring of rolling circle amplification (RCA) products on near-infrared (NIR) light-responsive gold nanoflowers (AuNFs). The incorporation of AS1411 aptamer into the RCA product endowed the nanoplatform with the capability of multivalent targeted binding to cancer cells. The nanoplatform exhibited good biocompatibility, notable NIR absorption and robust photothermal conversion, thereby enabling controlled drug release in response to dual NIR/pH stimulation. The developed nanoplatform holds great promise for targeted and responsive cancer treatments.
研究领域:
生物医学工程
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肿瘤学
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纳米医学
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DOI
高影响因子
Journal of Materials Chemistry A
·
2020
·Vol. 9
·期 3
·1835-1844
·
IF 11.301
Qin Zhang,Xin Liu,Lijie Duan,Guanghui Gao
Skin-mimicking electronics have aroused extensive interest in personalized health monitoring, human movement detections, and biomedical implants. However, the existing flexible electronics are still unable to compare with the advanced performance of human skin, and it remains challenging to achieve a high skin-mimicking capability. Herein, we design a DNA-inspired hydrogel mechanoreceptor with skin-like perception and mechanical behavior, as well as excellent biocompatibility. The hydrogels are constructed using a DNA-inspired adenosine monophosphate crosslinked quaternized chitosan network and a NaCl-containing polyacrylamide network. The hydrogels possess a low modulus, high toughness, self-stiffness, and fast self-recovery. As a result, the hydrogel mechanoreceptors exhibit high sensitivity toward strain and pressure, and present negligible electromechanical hysteresis even at a big deformation (strain of 500%). Impressively, the hydrogels can function as reliable wearable sensors for detecting whole-body movements and physiological signals, including various joint motions, facial emotions, vocalization, and respiration. It is anticipated that the DNA-inspired hydrogel mechanoreceptors would fuel the development of new generation of flexible electronics, such as electronic skin, medical implants, soft robotics, and flexible touchpads.
研究领域:
生物医学工程
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材料科学
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传感器技术
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DOI
高影响因子
ACS Nano
·
2023
·
IF 17.1
Cuiping Mao,Shuchen Wang,Jiankai Li,Zhuowei Feng,Tong Zhang,Rui Wang,Chunhai Fan,Xingyu Jiang
DNA as an exceptional data storage medium offers high information density. However, DNA storage requires specialized equipment and tightly controlled environments for storage. Fast encapsulation within minutes for enhanced DNA stability to do away with specialized equipment and fast DNA extraction remain a challenge. Here, we report a DNA microlibrary that can be encapsulated by metal–organic frameworks (MOFs) within 10 min and extracted (5 min) in a single microfluidic chip for automated and integrated DNA-based data storage. The DNA microlibrary@MOFs enhances the stability of data-encoded DNA against harsh environments. The encoded information can be read out perfectly after accelerated aging, equivalent to being readable after 10 years of storage at 25 °C, 50% relative humidity, and 10 000 lx sunlight radiation. Moreover, the library enables fast retrieval of target data via flow cytometry and can be reproduced after each access.
研究领域:
材料科学
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生物技术
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数据存储技术
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化学工程
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PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Yeru Wang,Yu Wang,Su Liu,Wenyu Sun,Manru Zhang,Long Jiang,Minghan Li,Jinghua Yu,Jiadong Huang
DNA walkers, as intelligent artificial DNA nanomachines, have been widely used as efficient nucleic acid amplification tools that the detection sensitivity can be improved by incorporating DNA walkers into DNA biosensors . Nevertheless, since the premature release or flameout in a region of locally exhausted substrate, the walking efficiency of DNA walkers remains unsatisfactory. In this work, we design a smart tripedal DNA walker that is formed by target-initiated catalyzed hairpin assembly (CHA), which can move along the DNA duplex tracks on electrode driven by toehold-mediated DNA strand displacement (TMSD) for transduction and amplification of electrochemical signals. Emphatically, this flexible tripedal DNA walker is capable of walking freely along the tracks with unconstrained walking range. Moreover, the design of multi-legged walker can weaken the derailment of leg DNA and shorten the moving time on electrode, ensuring the processive walking with high efficiency. Additionally, the persistent walking of tripedal walker is driven by cascading TMSD, which eliminates the defects of high cost and instability of enzyme-assisted amplification technology . Therefore, the tripedal DNA walker-based electrochemical biosensor has enormous potential for the applications of OTA detection, and reveals a new avenue for food safety analysis and clinical diagnosis.
研究领域:
生物纳米技术
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食品安全分析
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临床诊断
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DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Yaping Xia,Yang Sun,Huamin Li,Shuo Chen,Tiying Zhu,Guangcan Wang,Baoyuan Man,Jie Pan,Cheng Yang
Room-temperature plasma treated graphene based FET was firstly proposed for the DNA hybridization detection. Affinity and electrical properties of the graphene based DNA-FET sensor were studied and improved benefits from the surface modification. The facile room-temperature Ar plasma easily removes residues from the graphene surface and changes the hydrophilic properties of graphene, which is important for our solution gated DNA-FET sensor. Limit of the detection of below 10 aM is obtained in our experiment. Especially, DNA concentration (C DNA )/the amount of net drain current (ΔI) and the negative shift in the V CNP value of the GFET sensor with the plasma treated 30 s are all improved compared with that without treatment. It shows that the easily plasma treatment of the graphene surface can be used for the solution gated FET sensor.
研究领域:
材料科学
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生物传感器
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纳米技术
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电子工程
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生物化学
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高影响因子
PLANT JOURNAL
·
2018
·Vol. 94
·期 2
·288-303
·
IF 5.775
Jie Qian,Yueyue Chen,Ying Hu,Yingtian Deng,Yang Liu,Gang Li,Wenxuan Zou,Jie Zhao
Summary Replication factor C (RFC) is a conserved eukaryotic complex consisting of RFC1/2/3/4/5. It plays important roles in DNA replication and the cell cycle in yeast and fruit fly. However, it is not very clear how RFC subunits function in higher plants, except for the Arabidopsis ( At ) subunits AtRFC1 and AtRFC3 . In this study, we investigated the functions of AtRFC4 and found that loss of function of AtRFC4 led to an early sporophyte lethality that initiated as early as the elongated zygote stage, all defective embryos arrested at the two- to four-cell embryo proper stage, and the endosperm possessed six to eight free nuclei. Complementation of rfc4-1 /+ with AtRFC4 expression driven through the embryo-specific DD45pro and ABI3pro or the endosperm-specific FIS2pro could not completely restore the defective embryo or endosperm, whereas a combination of these three promoters in rfc4-1 /+ enabled the aborted ovules to develop into viable seeds. This suggests that AtRFC4 functions simultaneously in endosperm and embryo and that the proliferation of endosperm is critical for embryo maturation. Assays of DNA content in rfc4-1 /+ verified that DNA replication was disrupted in endosperm and embryo, resulting in blocked mitosis. Moreover, we observed a decreased proportion of late S-phase and M-phase cells in the rfc4-1 /– FIS 2; DD 45; ABI 3pro::At RFC 4 seedlings, suggesting that incomplete DNA replication triggered cell cycle arrest in cells of the root apical meristem. Therefore, we conclude that AtRFC4 is a crucial gene for DNA replication.
研究领域:
植物学
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遗传学
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分子生物学
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PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Xudong Wang,Jun Dai,Xuehong Min,Zhihua Yu,Yong Cheng,Kaixun Huang,Juliang Yang,Xiaoqing Yi,Xiaoding Lou,Fan Xia
Detection of an ultralow concentration of mRNA is important in the prognosis of gene-related diseases. In this study, a DNA-conjugated amphiphilic aggregation-induced emission probe (TPE-R-DNA) was synthesized for cancer tissue imaging and prognosis analysis based on an exonuclease III-aided target recycling technique. TPE-R-DNA comprise two components: a hydrophobic component that serves as the “turn-on” long wavelength fluorescence imaging agent (TPE-R-N3); and a hydrophilic single DNA strand (Alk-DNA) which acts as specific recognition part for target mRNA. In the absence of target mRNA, TPE-R-DNA had almost no fluorescence because of its high water solubility. Conversely, the TPE-R-DNA was digested by exonuclease III (Exo III) in the presence of MnSOD mRNA to release the hydrophobic fluorogens (TPE-R-AT). Subsequently, TPE-R-AT formed aggregates, and therefore, fluorescence signal was distinctly observed. For the first time, the structure of the hydrolysis product (TPE-R-AT), containing two bases A and T, was proved by the mass spectrum (MS) and high-performance liquid chromatography (HPLC). Moreover, the detection limit toward mRNA could be achieved in as low as 0.6 pM. Furthermore, the fluorescent signal can be used to confirm the MnSOD mRNA expression level in cancer tissue. The MnSOD mRNA expression in renal cancer was lower than in renal cancer adjacent tissue. In particular, the expression level was analyzed to predict prognosis of cancer patients. Our results demonstrate that a shorter survival time was evident among patients in lower MnSOD mRNA expression. Thereby, it indicates great potential for the development of an ultrasensitive biosensing platform for the application in disease prognosis.
研究领域:
癌症研究
·
分子生物学
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生物医学工程
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分析化学
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 46
·18251-18258
·
IF 3.069
Pei Yin,Tao Wang,Yuan Yang,Weidong Yin,Shaoxiong Zhang,Zengming Yang,Chunxuan Qi,Hengchang Ma
The chemical structures or positive charge-guided specific cell organelles' staining was an interesting but very challenging research work. In this paper, we synthesized a series of triphenylamine (TPA) derivatives with mono-, di- and tri-pyridinium as substituent groups. By exchanging the negative ions (I−, BF4− and PF6−) between them, a platform containing small compounds was established. After that, mammalian cells of HeLa and plant cells of BY-2 were employed for the study on cell imaging. The results clearly demonstrated that mono-pyridinium derivatives (TPA-Py-I−, TPA-Py-BF4− and TPA-Py-PF6−) are mitochondrion-targeted (for HeLa cells) and cell wall-targeted (for BY-2 cells). However, both di- and tri-pyridinium substituted derivatives (TPA-diPy-BF4− and TPA-triPy-BF4−) were nucleus-targeted for both cell lines. From these important findings, we can conclude that the quantity of positive charge plays a decisive role in different cell organelle-targeted staining. We also declared that our research results were very useful for designing and synthesizing probes with good cellular uptake capability, significantly enhanced targeting ability, and DNA detection.
研究领域:
细胞生物学
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分子生物学
·
化学
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高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Chunyuan Song,Xinyu Jiang,Yanjun Yang,Jingjing Zhang,Steven Larson,Yiping Zhao,Lianhui Wang
A novel surface-enhanced Raman scattering/surface plasmon resonance (SERS/SPR) dual-mode biosensor prepared on a silver nanorod-covered silver nanohole (Ag NR-NH) array by surface modification of tetrahedral DNA probes is proposed for highly sensitive detecting nucleic acids by a special signal amplification strategy of DNA supersandwich. The Ag NR-NH with a large area and uniformly arrayed nanostructure possesses excellent anisotropic extraordinary optical transmission and strong localized surface plasmon resonance, which lead to sensitive SPR response to the change of a local refractive index and strong localized electric fields for excellent SERS activity. To obtain high sensitivity and specificity, smart tetrahedral DNA probes are immobilized onto the Ag NR-NH array and the DNA supersandwich sensing strategy, including the signal amplification of DNA concatamers, is used. About 10 times signal enhancement for SPR and 4 times for SERS are achieved by this sensing strategy. In the detection of the target DNA in the human serum, the two sensing modes have complementary performances, i.e., the limit of detection for the SPR array is high (0.51 pM), while for SERS, it is low (0.77 fM), but the specificity for SPR is much higher than that of SERS. This improves the robustness of the DNA sensors, and subsequent recovery tests also confirm good reliability of the biosensor. The proposed SERS/SPR dual-mode biosensor has a great potential for high performance and reliable detection of trace disease-related nucleic acid biomarkers in the serum and is a powerful sensing platform for early-stage disease diagnosis.
研究领域:
分析化学
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生物传感
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纳米技术
·
分子诊断
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）