Cell Death Discovery
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2025
·
IF 7
Rui Ma,Chen Peng,Wenjuan Chen,Yizhen Zhang,Yangfeng Ding,Xin Wang,Yuling Shi
The skin barrier is crucial for protecting against environmental challenges, preventing water loss, and regulating immune responses. This study aims to investigate the roles and mechanisms of SERPINB7 in skin barrier maintenance. We found that SERPINB7 deficiency disrupts tight junctions of keratinocytes in vitro, and specific knockout of Serpinb7 in keratinocytes impairs skin barrier function in vivo. SERPINB7 deficiency leads to reduced expression of O-GalNAc regulatory proteins and structural abnormalities in the Golgi apparatus, ultimately impairing protein O-GalNAc glycosylation. Legumain acts as a critical mediator in the maintenance of normal biological functions and O-GalNAc glycosylation regulated by SERPINB7. O-GalNAc inhibition exhibits biological effects analogous to those induced by SERPINB7 deficiency, leading to weakened tight junctions, reduced cell adhesion, and compromised skin barrier integrity in keratinocytes and mouse skin, respectively. Consequently, O-GalNAc deficiency exacerbates inflammatory skin diseases such as psoriasis and atopic dermatitis. Mechanistically, O-GalNAc deficiency primarily affects the glycosylation of calcium-related and cell adhesion-related proteins, disrupting calcium signaling and compromising cell adhesion, ultimately leading to skin barrier dysfunction. In summary, this study demonstrates that SERPINB7 maintains skin barrier through protein O-GalNAc glycosylation. These findings not only deepen our understanding of skin barrier biology but also provide new insights for developing therapeutic strategies for skin barrier-related diseases.
研究领域:
皮肤病学
·
分子生物学
·
糖生物学
·
细胞生物学
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Chem & Bio Engineering
·
2026
·
IF 8.4
He Qin,Cheng Shuai,Yue Jianqing,Zong Shu,Li Congyu,Qi Xuerong,Liu Lipei,Zhang Zhidan,Sun Jun,Cheng Jian,Lu Xiaoyun,Jiang Huifeng
3 ′ -O-amino-deoxyribonucleoside
triphosphates (3 ′ -ONH 2 -dNTPs) are crucial reversible
terminators for template-independent
DNA synthesis. Owing to the structural complexity of purine bases,
here we propose a chemo-enzymatic process for the synthesis of purine-type
3 ′ -ONH 2 -dNTPs. First, the aminoalkoxyl group was
introduced into commercially available dA and dG via a chemical catalytic
process, affording yields of 24.9% and 27.3%, respectively. Subsequently,
to establish a green and scalable enzymatic cascade for phosphorylation
of the substrates, we engineered two key phosphorylation enzymes (Dm-dNK
and MrPPK2), improving the rate-limiting monophosphorylation of 3 ′ -ONH 2 -dG from 26% to 97.8% and achieving 59.9% conversion to the
corresponding triphosphate. Lastly, the produced nucleotides exhibit
performance equivalent to commercial counterparts in terminal deoxynucleotidyl
transferase (TdT)-mediated DNA synthesis, supporting the construction
of 120-nt strands with ∼99.7% stepwise accuracy. This work
establishes an efficient and generalizable catalytic platform for
producing modified dNTPs, advancing the practical application of enzymatic
DNA synthesis technologies.
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JOURNAL OF ORGANOMETALLIC CHEMISTRY
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2019
·
IF 2.066
Ke Cao,Cai-Yan Zhang,Tao-Tao Xu,Ji Wu,Li-Fang Ding,Linhai Jiang,Junxiao Yang
The palladium catalyzed/counter ion tuned selective methylation of 9-amide- o -carboranes on B(4) and B(12) has been developed, and a series of o -carborane derivatives decorated with various groups have been synthesized with moderate yields. The in situ formed palladium species via counter ion exchange was proposed for the tunable B–H activation.
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CARBOHYDRATE POLYMERS
·
2011
·Vol. 85
·期 4
·832-837
·
IF 3.463
Dongke Zhao,Jun Huang,Sheng Hu,Jianwei Mao,Lehe Mei
Chitosan was prepared by alkaline N -deacetylation of squid cartilage β-chitin and carboxymethylated derivatives with different degrees of substitution (DS) were synthesized. The DS of the derivatives calculated by pH titration were 0.64, 0.81, 1.0, 1.33 and 1.59. The antioxidant properties and bile acid binding capacity of the derivatives were studied in vitro . The carboxymethylation of chitosan caused enhancement of bile acid binding capacity. At 1 mg/mL, carboxymethyl chitosan (CMCS) showed a stronger scavenging effect than chitosan towards DPPH radicals. The CMCS scavenging effect on superoxide radicals was stronger than that of chitosan, and EC 50 values were below 5.6 mg/mL. The effectiveness of reducing power correlated with the DS of CMCS. At 1.2 mg/mL, the ability of CMCS to chelate ferrous iron was 100%, whereas that of chitosan was only 22.34%. This suggested that carboxymethylation is a possible approach to obtain chitosan derivatives with desirable biological properties.
研究领域:
生物化学
·
海洋生物技术
·
食品科学
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高分子化学
·
功能生物材料
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JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2023
·
IF 3.6
XiaoNan Zhang,WenQin Xu,Xing Li,GuangHua Pan,NanChun Chen,QingLin Xie,XiuLi Wang
This experiment was done to study the zeolite molecular sieve as a drug-binding effector, the non-antibiotic drug potassium diformate uniformly disperse in the internal aqueous phase of the 'egg box' structure formed by pectin-calcium ions. With oil phase as the intermediate phase and Xanthan gum Chitosan as the external water phase, the W/O/W type sustained release bacteriostatic microcapsules with pH response were prepared and characterized by Fourier transform infrared, thermogravimetric, SEM, and TEM. It can be obtained through characterization experiments that the inner water phase, oil phase, and outer water phase were formed by observation, and W/O/W emulsion microcapsules were obtained and the bacteriostasis effect of microcapsules was verified by bacteriostasis experiment. The permeance experiment showed that the molecular sieve was successfully coated in the microsphere. Studying on drug release mechanism and sustaining release performance of composite emulsion microcapsules. In vitro drug release study showed that the encapsulation efficiency and drug loading rate of microcapsules were improved by adding molecular sieve, reaching 12.31% and 61.55%, respectively. At the same time, we observed that the drug release rate slowed down during the simulated intestinal release process, and the drug release kinetics were in line with the first-order kinetic model and Ritger-Peppas model equation. Experiments had proven that the drug-loaded microcapsules exerted a significant bacteriostatic effect on Escherichia coli, Staphylococcus aureus, and Bacillus subtilis, with the highest antibacterial rates of 97.25%, 94.05%, and 95.93%, respectively. Therefore, the composite emulsion microcapsules can be used as a new controlled-release drug delivery system in vivo.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2019
·
IF 4.084
Yanhui Guo,Ruiqi Zhang,Weiju Hao,Jiankan Zhang,Yanjing Yang
The development of efficient and non-noble catalyst is of great significance to hydrogen generation techniques. Three surface-oxidized cobalt borides of Co–B–O@Co x B (x = 0.5, 1 and 2) have been synthesized that can functionalize as active catalysts in both alkaline water electrolysis and the hydrolysis of sodium borohydride (NaBH 4 ) solution. It is discovered that oxidation layer and low boron content favor the oxygen evolution reaction (OER) activity of Co–B–O@Co x B in alkaline water electrolysis. And surface-oxidized cobalt boride with low boron content is more active toward hydrolysis of NaBH 4 solution. An alkaline electrolyzer fabricated using the optimized electrodes of Co–B–O@CoB 2 /Ni as cathode and Co–B–O@Co 2 B/Ni as anode can deliver current density of 10 mA cm −2 at 1.54 V for overall water splitting with satisfactory stability. Meanwhile, Co–B–O@Co 2 B affords the highest hydrogen generation rate of 3.85 L min −1 g −1 for hydrolysis of NaBH 4 at 25 °C.
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SENSORS
·
2017
·Vol. 17
·期 3
·530
·
IF 2.677
Ru-Jia Yu,Jia-Jia Sun,Heng Song,Jing-Zhi Tian,Da-Wei Li,Yi-Tao Long
Real-time monitoring of chemical reactions is still challenging as well as important to study reaction mechanisms and reaction kinetics. Herein, we demonstrated the real-time monitoring of o-phenylenediamine (OPD) oxidation on the surface of gold nanoparticles by surface-enhanced Raman spectroscopy (SERS). The oxidation mechanism and the reaction kinetics were investigated on the basis of the SERS spectrum variation and the related density functionalized theory calculation. It was shown that the oxidation of OPD in the presence of copper ions was a two-step process of the deprotonation of the amino group on the aromatic rings and the rearrangement of the electron cloud to a π-conjugated system, which may open a new door to comprehensively understand the reaction process.Keywords:surface-enhanced Raman spectroscopy;oxidation of o-phenylenediamine;real-time monitoring
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-17
·
IF 15.7
Liu Qiannan,Lu Xiaoyan,Deng Yao,Zhang Han,Wei Rumeng,Li Hongrui,Feng Ying,Wei Juan,Ma Fang,Zhang Yan,Zou Xia
Protein O-glycosylation plays critical roles in sperm formation and maturation. However, detailed knowledge on the mechanisms involved is limited due to lacking characterization of O-glycoproteome of testicular germ cells. Here, we performed a systematic analysis of site-specific O-glycosylation in mouse testis, and established an O-glycoproteome map with 349 O-glycoproteins and 799 unambiguous O-glycosites. Moreover, we comprehensively investigated the distribution properties of O-glycosylation in testis and identified a region near the N-terminal of peptidase S1 domain that is susceptible to O-glycosylation. Interestingly, we found dynamic changes with an increase Tn and a decrease T structure from early to mature developmental stages. Notably, the importance of O-glycosylation was supported by its effects on the stability, cleavage, and interaction of acrosomal proteins. Collectively, these data illustrate the global properties of O-glycosylation in testis, providing insights and resources for future functional studies targeting O-glycosylation dysregulation in male infertility.
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CHEMBIOCHEM
·
2024
·
IF 2.6
Wen Fu,Zhongli Shao,Zhiping Xu,Zhong Li,Xusheng Shao
Graphical NP-TBZ is developed by covalently linking o -nitrobenzyl ( NP ) with thiabendazole ( TBZ ). Compound NP-TBZ can be controlled to release TBZ in dependent to light, which resulted in controllable in-vivo activity against wheat scab. It is promising for spatiotemporal controlled-release of TBZ in factual applications, posing a theoretical guidance in development of controlled-release pesticides. Pesticides are essential in agricultural development. Controlled-release pesticides have attracted great attentions. Base on a principle of spatiotemporal selectivity, we extended the photoremovable protective group (PRPG) into agrochemical agents to achieve controllable release of active ingredients. Herein, we obtained NP-TBZ by covalently linking o -nitrobenzyl ( NP ) with thiabendazole ( TBZ ). Compound NP-TBZ can be controlled to release TBZ in dependent to light. The irradiated and unirradiated NP-TBZ showed significant differences on fungicidal activities both in vitro and in vivo. In addition, the irradiated NP-TBZ displayed similar antifungal activities to the directly-used TBZ , indicating a factual applicability in controllable release of TBZ . Furthermore, we explored the action mode and microcosmic variations by SEM analysis, and demonstrated that the irradiated NP-TBZ retained a same action mode with TBZ against mycelia growth.
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JOURNAL OF MOLECULAR STRUCTURE
·
2011
·
IF 1.599
Bin Peng,Yong Nie,Jinling Miao,Zhenwei Zhang,Meiling Xu,Guoxin Sun
The reaction of o -carboranyllithium with two equiv. of 2-pyridinecarboxaldehyde affords 1-[(hydroxy)(pyridyl)methyl]- o -carborane ( 1 ) and 1,2-bis[(hydroxy)(pyridyl)methyl]- o -carborane ( 2 ), which have been characterized by IR and NMR spectroscopy, mass spectrometry and single-crystal X-ray diffraction. Compound 2 exhibits interesting polymorphism (monoclinic 2a and triclinic 2b ). The solid state structures of the new carboranes feature intermolecular and intramolecular hydrogen bonding interactions involving all the N and O atoms, leading to one dimensional ( 1 , 2b ) or two-dimensional ( 2a ) supramolecular structures. The photoluminescence of 1 and 2b was investigated in the solid and solution states at room temperature, respectively.
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CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Yee-Shin Chang,Cheng-Xin You,Tai-Chen Kuo
In this study, SrY₂O₄:Dy³⁺ phosphors were synthesized using the solid-state reaction method and evaluated after heat treatment at 1300 °C for 5 h. XRD analysis confirmed the orthorhombic structure of SrY₂O₄ with residual Y₂O₃ present. The addition of Dy³⁺ ions caused lattice expansion, impacting grain size and crystallinity. SEM images showed that the phosphor particles had irregular shapes with minimal size variation, although they tended to agglomerate due to high-temperature heat treatment. Optical analysis revealed significant absorption and emission peaks, with a strong excitation band in the 200–306 nm range and prominent sharp peaks in the 306–480 nm range, particularly at a 0.5 mol% doping concentration. Emission spectra indicated the highest intensity at 0.5 mol% Dy³⁺ concentration, with intensity decreasing due to concentration quenching at higher levels. Thermal stability tests showed peak emission intensity at 70 °C, with stability maintained up to 190 °C before notable thermal quenching. Chromaticity coordinates remained within the warm white region at both temperatures. This study provides detailed insights into the effects of Dy³⁺ doping on the structural, optical, and thermal properties of SrY₂O₄ phosphors. Compared to previous studies on rare-earth-doped SrY₂O₄ phosphors, this work is the first to systematically explore the substitution of Y³⁺ ions with Dy³⁺ ions until full replacement. The study not only confirmed that the highest emission intensity occurs at 0.5 mol% Dy³⁺ concentration, but also demonstrated that Dy³⁺-doped SrY₂O₄ phosphors exhibit excellent thermal stability, with emission stability maintained below 150 °C. These findings expand the potential applications of Dy³⁺-doped SrY₂O₄ phosphors, particularly in high-temperature environments and solid-state lighting.
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Journal of Energy Storage
·
2025
·
IF 9.8
Qianqian Zhang,Yaru Zhu,Min Yang,Yanru Zhang,Jialin Wang,Shanshan Liu,Ye Liu,Xin Yang,Kai Feng,Wenzuo Li
Aqueous zinc-ion batteries (AZIBs) have good application prospects in the field of large-scale energy storage. The design and synthesis of high-performance cathode materials are key to promoting the development and application of AZIBs. In this work, the combination of redox active V 4+ cation and HPO 3 2− anion provides excellent zinc-ion (de)intercalation ability. In addition, HPO 3 2− and C 2 O 4 2− anion groups collaborate to construct a stable framework with multi-dimensional zinc-ion transport channels, providing fast and robust pathway for zinc-ion transport. Carbon composites further improve electronic conductivity, shorten ion transport distance, and increase the electrochemical reaction area of Na 2 [(VO) 2 (HPO 3 ) 2 (C 2 O 4 )]·2H 2 O@C (NVPCO@C) cathode material. As a result, NVPCO@C cathode material delivers a high specific capacity over 180 mAh·g −1 at a current density of 40 mA·g −1 . Moreover, NVPCO@30%C cathode material maintains 100 % capacity after 100 cycles. Ex-situ X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and Raman spectra confirm the electrochemical activity of V 4+ /HPO 3 2− ions and reveal the zinc-ion storage mechanism.
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