Small
·
2024
·
IF 13
Lihua Chen,Hui Ming,Bowen Li,Chen Yang,Shanshan Liu,Yajie Gao,Tingting Zhang,Canhua Huang,Tingyuan Lang,Zhuo Yang
Cancer metastasis poses significant challenges in current clinical therapy. Osthole (OST) has demonstrated efficacy in treating cervical cancer and inhibiting metastasis. Despite these positive results, its limited solubility, poor oral absorption, low bioavailability, and photosensitivity hinder its clinical application. To address this limitation, a glutathione (GSH)-responded nano-herb delivery system (HA/MOS@OST&L-Arg nanoparticles, HMOA NPs) is devised for the targeted delivery of OST with cascade-activatable nitric oxide (NO) release. The HMOA NPs system is engineered utilizing enhanced permeability and retention (EPR) effects and active targeting mediated by hyaluronic acid (HA) binding to glycoprotein CD44. The cargoes, including OST and L-Arginine (L-Arg), are released rapidly due to the degradation of GSH-responsive mesoporous organic silica (MOS). Then abundant reactive oxygen species (ROS) are produced from OST in the presence of high concentrations of NAD(P)H quinone oxidoreductase 1 (NQO1), resulting in the generation of NO and subsequently highly toxic peroxynitrite (ONOO − ) by catalyzing guanidine groups of L-Arg. These ROS, NO, and ONOO − molecules have a direct impact on mitochondrial function by reducing mitochondrial membrane potential and inhibiting adenosine triphosphate (ATP) production, thereby promoting increased apoptosis and inhibiting metastasis. Overall, the results indicated that HMOA NPs has great potential as a promising alternative for the clinical treatment of cervical cancer.
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PubMed
高影响因子
Frontiers in Veterinary Science
·
2025
·
IF 2.9
Chen Fan,Changzheng Chen,Renping Liu,Muredili Maimaiti,Changgeng Li,Weijie Zao,Waike Lv,Guozhu Xu,Guodong Zhao
Background To investigate the impact of L -arginine ( L -Arg) and L -citrulline ( L -Cit) on cow reproductive performance. Methods 90 healthy, age-matched Simmental cows were randomly assigned to three groups: control, L -Arg, and L -Cit, with 30 cows per group. Estrus synchronization was performed, and various parameters were assessed, including estrus rate, conception rate, and early embryo apoptosis, following intraperitoneal administration of L -Arg and L -Cit. Additionally, reproductive hormones, serum antioxidant levels, and plasma metabolites were measured. Results Results indicated that both the estrus and conception rates in the L -Arg and L -Cit groups were significantly higher than those in the control group ( p < 0.05). Furthermore, serum LH levels and VEGF concentrations on day 1 and day 8 were elevated in the L -Arg and L -Cit groups compared to controls ( p < 0.05). Estrus rate and serum NO concentration in the L -Cit group were notably higher than those in the control group ( p < 0.05). At day 8 and day 21, estrus rate, conception rate, P levels, and serum VEGF concentrations were significantly greater in both the L -Arg and L -Cit groups compared to controls ( p < 0.05). Additionally, serum NO concentration was significantly higher in the L -Cit group than in the control group ( p < 0.05). The activities of T-AOC and GSH-Px in serum were significantly increased in both primiparous and multiparous cows in the L -Arg and L -Cit groups relative to the control group ( p < 0.05). Metabolomic analysis revealed that in primiparous cows, the protein digestion and absorption, ABC transporters, and AMPK signaling pathways were significantly enriched. In multiparous cows, pyrimidine metabolism was notably enriched. Conclusion Under the experimental conditions, L-Arg modulated the production of Dimethyl sulfone, 1,5-pentanediamine, Bassanolide, and Phenylalanine in primiparous cows through the protein digestion and absorption and ABC transporters pathways, while L -Cit influenced the same metabolites via the ABC transporters pathway. Both L -Arg and L -Cit promoted increased NO, GSH-Px, T-AOC, and VEGF levels, enhancing early embryo implantation. Additionally, L -Arg and L -Cit synergistically influenced pyrimidine metabolism, regulating the production of N-myristoylphosphorylcholine, N-oleoyl-d-erythro-sphingosylphosphorylcholine, 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine, and 1-docosahexaenoyl- 2-stearoyl-sn-glycero-3-phosphocholine. This modulation resulted in increased NO, GSH-Px, T-AOC, and VEGF in multiparous cows, ultimately supporting early embryo implantation.
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PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2026
·
IF 2.5
Jingjie Hu,Guofu Zeng,Xiaoyan Long,Xiaoyan Lin
Amidated collagen (A-COL) with good biocompatibility was synthesized via carbodiimide-mediated coupling of L-arginine to native collagen (COL). The structure and charge type of A-COL and COL were characterized by FTIR, zeta potential analysis and elemental analysis (EA). A-COL was incorporated into the foam detergent as both an antibacterial agent and foam stabilizer. The antibacterial activities of A-COL against E. coli and S. aureus were analyzed, along with its impact on, and mechanism for enhancing, the detergent's foaming capacity and foam stability. The results showed that A-COL inhibited both bacteria, with MIC values of 0.312 mg mL−1 and 0.078 mg mL−1, respectively. The antimicrobial mechanism of A-COL involves its positively charged and synergistically active structure, which enhances electrostatic interactions and destroys the cell's integrity. Foam stability with A-COL was 1.43-fold higher than without A-COL. The addition of A-COL enhances the foam stability of the foam detergent through its long-chain molecules forming an interweaving network structure. The detergent containing A-COL exhibited no skin irritation, excellent biocompatibility, and a predicted shelf life of over 3 years as determined by accelerated thermal stability testing. The dual-functional A-COL significantly enhances the detergent's antibacterial efficacy, storage stability, foam-stabilizing capacity, and biocompatibility, demonstrating considerable potential for application in biomass-based detergents.
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高影响因子
AQUACULTURE NUTRITION
·
2026
·Vol. 2026
·期 1
·4395252
·
IF 4.6
Haoran Yin,Lixing Wang,Zexin Lin,Ruilin Li,Qinxiao Cai,Zhiyu Zhou,Xiaoyi Wu
The hybrid grouper ( Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂), a typical carnivorous fish, has a limited ability to utilize dietary carbohydrates (CHOs). This study investigated the effects of different dietary arginine (Arg) levels (2.55 and 3.65%) and CHO-to-lipid ratios (C/L: 3.57, 2.39, 1.61) on the glycometabolism of hybrid grouper. Juvenile hybrid grouper (mean initial body length and weight: 7.8 cm and 13.04 g) were manually fed to fullness two times per day throughout a 7-week growth trial. The results showed that the C/L3.67 diets significantly elevated 2-h postprandial blood glucose concentration, as well as reduced hepatic glycogen level, intraperitoneal fat ratio (IPF), and the whole-body and hepatic lipid contents relative to the C/L2.39 and C/L1.61 diets. Dietary Arg supplementation significantly improved this metabolic disorder. These improvements were associated with enhanced plasma insulin-like growth factor 1 (IGF-1) concentration, hepatic phosphofructokinase (PFK) activity, nitric oxide (NO), and cyclic guanosine monophosphate (cGMP) levels. They were also linked to the upregulated expression of key molecules in related pathways, including IGF-1 receptor (igf1r), phosphatidylinositol 3-kinase (pi3k), protein kinase AMP-activated catalytic subunit α (ampk-α), and protein kinase B (Akt) genes, as well as glucose transporter 4 (GLUT4) and mechanistic target of rapamycin (mTOR) proteins. Generally, suitable dietary Arg supplementation can reduce postprandial blood glucose and hepatic glycogen concentrations in hybrid grouper fed high C/L diets by regulating key genes and proteins within the IGF-1 and NO-cGMP pathways. This finding provided a nutritional intervention to enhance CHO utilization in hybrid grouper aquaculture.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yingqi Liang,Yupeng Liu,Jun Wu,Lingyun Li,Xue Wu,Zekun Yan,Mingyu Chen,Elena V. Ushakova,Caishi Huang,Songnan Qu
The development of stimuli-responsive fluorescent nanomaterials with molecular pores is crucial for real-time monitoring of the delivery of functional non-fluorescent small molecules. Herein, a new type of yellow emissive porous carbon dots (CDs) derived from 3,9-perylenedicarboxylic acid was synthesized, employing a radical-assisted method under ambient pressure and mild heating conditions, and it is the smallest porous nanoplatform based on CDs. The molecular pores in the CDs can absorb L-arginine via intermolecular hydrogen bonds to form CDs and L-arginine complex (Arg@CDs). The absorbed L-arginine molecules in the molecular pores break the interlayer π – π interactions in the core due to the steric hindrance, leading to a fluorescence color shift from yellow to cyan. When exposed to an acidic environment, the absorbed L-arginine can be released, resulting in a recovery of yellow fluorescence. In vitro, Arg@CDs can be endocytosed by cells and localized to lysosomes, in which the acidic environment causes L-arginine release from the Arg@CDs with a fluorescence color change from cyan to yellow. Thus, Arg@CDs can be used as novel fluorescence color-sensitive pH probe for intracellular dynamic fluorescent imaging. Furthermore, in the mouse scald wound model, intravenous injection of Arg@CDs can significantly accelerate wound healing by promoting macrophage proliferation and anti-inflammatory action.
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高影响因子
BIOELECTROCHEMISTRY
·
2020
·
IF 4.722
Yumin He,Li Zhou,Lei Deng,Zemeng Feng,Zhong Cao,Yulong Yin
As a primary building block for protein synthesis, l -arginine ( l -Arg) is also a precursor for the synthesis of important metabolites, and is involved in various physiological and pathophysiological processes. l -Arg is a potential biomarker in clinical diagnosis and nutritional status assessment, making it valuable to quantify and monitor this biomolecule. In this study, peptide aptamers that specifically interact with l -Arg were identified by high-throughput molecular docking, and the binding capacities between the synthesized peptide aptamers and l -Arg were then measured by isothermal titration calorimetry. We hypothesized that the peptide aptamer with the greatest binding capacity could be used as the recognition element in a biosensor. A chemosynthetic peptide aptamer modified with mercaptan and spacer units (thioctic acid-GGGG-FGHIHEGY) was thus used to construct label-free electrochemical impedimetric biosensors for l -Arg based on gold electrodes. The optimum biosensor showed good sensitivity to l -Arg with a linear range of 0.1 pM–0.1 mM, and the calculated limit of detection (three times the signal-to-noise ratio) was 0.01 pM. Interference studies and assays of diluted serum samples were also carried out, and satisfactory results obtained. In conclusion, a potential method of peptide aptamer screening and biosensor fabrication for detecting small biological molecules was demonstrated.
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高影响因子
JOURNAL OF FLUORESCENCE
·
2016
·Vol. 27
·期 1
·293-302
·
IF 1.601
Li Na,He Yu,Ge Yili,Song Gongwu
In this report, a novel one-step chemical reduction method was reported for synthesis of water-soluble and stable fluorescent glutathione-templated silver nanocluster (GSH-Ag NCs) with ascorbic acid as an environmental-friendly reducing agent. On the basis of an oxidoreduction-induced fluorescence quenching mechanism, the prepared GSH-Ag NCs found to act as a cheap, non-toxic and highly sensitive “turn-off” fluorophore for ascorbic acid (AA). Furthermore, the fluorescence of the fluorophore/AA system could be recovered through addition of arginine (Arg), which made the system function as a highly selective “turn-on” sensor for arginine. Therefore, a “turn-off-on” switch sensor was proposed for detection of AA and Arg. Under optimized conditions, the probe gives a fluorescent response that is linear in the 2–300 μM concentration range of AA, with a detection limit of 0.1 μM. The probe for Arg, in turn, has a linear range in the 10–180 μM concentration range, and the limit of detection is 0.5 μM. In addition, the developed method showed great accuracy when employed to detect AA and Arg in human urine and serum, which shows its great potential in biological molecular recognition applications.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2012
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IF 2.905
Yamin Li,Zhuo Ye,Jing Zhou,Jie Liu,Ge Song,Kai Zhang,Baoxian Ye
A novel voltammetric sensor based on graphene–Nafion and poly( L -arginine) was constructed by electrodeposited and simple dipping–drying methods and used for high-sensitive determination of Terbutaline sulfate (TBS). The resulting poly( L -arginine)/graphene–Nafion modified electrode showed a significant voltammetric response to TBS due to the synergistic effect of poly( L -arginine) and graphene–Nafion, in which poly( L -arginine) had electrocatalytic ability for TBS and graphene provided a large specific surface area, excellent electric conductivity and electrocatalytic activity. The sensor gave a good linear range over 4.0 × 10 −8 –5.0 × 10 −6 mol L −1 with a detection limit of 1.2 × 10 −8 mol L −1 towards the determination of TBS. The high sensitivity and selectivity together with the good linear relationship, very easy surface regeneration and good stability at such a modified electrode led us to construct a practical TBS biosensor successfully. This work promoted the potential applications of graphene-based materials in electrochemical sensors.
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高影响因子
Scientific Reports
·
2026
·
IF 4.9
Wang Qi,Bi Pengfei,Wang Chunlin,Li Wenyang,Chi Bowen,Li Haitong,Tang Xin,Ma Tianyi
Colorectal cancer (CRC) often exhibits severe tolerance to immunotherapy due to the highly immunosuppressive nature of the tumor microenvironment (TME), with the polarization of tumor-associated macrophages (TAMs) toward the pro-tumor M2 phenotype playing a key role. Arginine (Arg), as a core precursor for the synthesis of the immune-microenvironment-reshaping metabolite spermine (Spm), is a crucial factor influencing the fate of TAMs. Unlike some tumors with Arg auxotrophy, CRC cells exhibited elevated argininosuccinate synthetase 1 (ASS1) expression, which was associated with increased extracellular Arg availability in our co-culture system. This study aimed to investigate the specific regulatory mechanisms of CRC cell metabolic reprogramming on immune function in the TME. Single-cell and clinical tissue analyses revealed elevated ASS1 expression in CRC epithelial cells and enrichment of SMS in M2-like TAMs, while higher spermine synthase (SMS) expression was associated with poorer survival in the analyzed PD-1-treated CRC cohort. The Transwell co-culture model demonstrated that CRC cells specifically upregulated ASS1 and released large amounts of Arg into the TME. Macrophages took up Arg and utilized their significantly enriched SMS to convert it into Spm. The intracellular accumulation of Spm further activated the PI3K-AKT-mTOR signaling pathway, driving macrophage polarization toward the M2 phenotype and upregulating immunosuppressive factors such as IL-10 and TGF-β, which in turn induced the exhaustion of local CD8 + T cells. Based on these findings, combined treatment with AMXT 1501 and Nivolumab attenuated M2-like polarization and the CD8⁺ T-cell exhaustion-associated phenotype, reduced CRC-cell proliferation, and increased apoptosis in vitro, with the major findings further validated in the MSS CRC cell line SW480. In conclusion, this study clarified the importance of the ASS1/Arg/SMS/Spm cross-cellular metabolic axis in CRC immune escape and demonstrated that targeting polyamine metabolism in combination with immune checkpoint blockade represents a promising strategy for overcoming immune tolerance across molecularly distinct CRC models.
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Ting Liu,Na Li,Jiang Xue Dong,Ying Zhang,Yu Zhu Fan,Shu Min Lin,Hong Qun Luo,Nian Bing Li
A bidimensional optical sensing platform which combines the advantages of fluorescence and colorimetry has been designed for arginine (Arg) detection. The system was established by monitoring the influence of Arg on the growth of gold nanoparticles/carbon quantum dots (Au/CQDs) composite, and the CQDs synthesized by ethylene glycol were used as the reducing and stabilizing agent in this paper. Considering that Arg is the only amino acid with guanidine group and has the highest isoelectric point (pI) value at 10.76, Arg would carry positive charges at pH 7.4. Consequently, the positively charged guanidine group of Arg could attract A u C l 4 − and CQDs through electrostatic interaction , which inhibited the growth of Au/CQDs composite. Thereby, the color of the system almost did not change and the fluorescence quenching of CQDs was prevented in the presence of Arg. Based on the color change a low detection limit for Arg was 37 nM, and a detection limit of 450 nM was obtained by fluorescence spectroscopy. Moreover, this dual-signal sensor also revealed excellent selectivity toward Arg over other amino acids. Besides, Arg can be detected in urine samples with satisfactory results, which demonstrate the potential applications for real analysis.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Haoran Wang,Wei Ji,Feng Xiao,Yewen Xiao,Linlin Li,Xia Shu,Hualin Wang,Jun Yin
Over-ripening and spoilage of fruits, driven by excessive ethylene (C 2 H 4 ) production and microbial proliferation, present critical challenges in fruit postharvest preservation. Herein, a multifunctional photocatalytic film, CTC-Arg@CS/HPMC, is developed by incorporating chlorophyll-TiO 2 @carbon sphere (CTC) photocatalyst and L-arginine (Arg) NO donor into chitosan/hydroxypropyl methylcellulose (CS/HPMC) matrix for banana preservation. The distinctive dual heterojunction structure of CTC enhances photocatalytic activity under visible light, promoting reactive oxygen species (ROS) generation that triggers NO release from Arg. Under combined action of ROS and NO, CTC-Arg@CS/HPMC can not only inactivate bacteria with high antibacterial efficiency against Escherichia coli (93 ± 3 %) and Staphylococcus aureus (99 ± 2 %) within 60 min but also delay fruit ripening through C 2 H 4 degradation and inhibition of biosynthesis. In practical application scenario, CTC-Arg@CS/HPMC can effectively extend banana shelf-life by over 100 % at 25 °C, highlighting its potential as an active packaging material for extending the postharvest life of climacteric fruits.
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JOURNAL OF CONTROLLED RELEASE
·
2024
·
IF 10.5
Yirou Wang,Yi Liu,Ji Chen,Zeyang Ge,Jing Wang,Dehua Li
Peri-implantitis, caused by bacterial biofilm on dental implants, leads to bone loss and tissue inflammation, ultimately causing oral health decline. Traditional methods to remove biofilm are ineffective in promoting reosseointegration on implant surfaces. This phenomenon can be attributed to two factors: incomplete removal of biofilm from hard-to-reach areas and alterations in the physicochemical properties of implant surfaces caused by decontamination procedures. To address this problem, we developed D-arginine-loaded chitooligosaccharide-capped pH-responsive mesoporous silica nanoparticles (Dar@MSN-COS) for improving the efficacy of decontamination of Water Jet (WJ). Dar@MSN-COS particles exhibit a targeted approach towards the extracellular polymeric substance (EPS) in order to disrupt the biofilm, and possess the capability to infiltrate confined areas between implant screws. Following this, the WJ treatment effectively removed residual biofilm and demonstrated improved cleaning efficacy. Furthermore, the decontamination of the Dar@MSN-COS combination with WJ promotes effective cell cytocompatibility on the titanium surface. The results of mechanistic experiments indicate that Dar@MSN-COS may act on biofilms by releasing a significant quantity of reactive oxygen species (ROS), suggesting it as a key contributing factor. In summary, our novel therapeutic protocol shows promise as an alternative solution for addressing the clinically complex aspects of peri-implantitis.
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