Artificial Cells Nanomedicine and Biotechnology
·
2017
·
IF 5.605
Yuchen Huang,Yi Zhang,Longbao Feng,Liumin He,Rui Guo,Wei Xue
When hydrophobically modified chitosan (HM-CHI) comes into contact with red blood cells (RBCs), it can cause agglomeration. This property leads HM-CHI to be a potential agent to form a rapid haemostatic plug to stop bleeding at wound sites. In this study, we investigated the properties of the HM-CHI biopolymer to act as an agent that rapidly clots blood. We have examined the synthesis and structural characteristics of HM-CHI – blood substances using X-ray diffraction, infrared spectroscopy and elemental analysis. To understand the rheological behaviour of RBCs within HM-CHI, we have studied the effects of HM-CHI on RBC aggregation and morphology using a rheometer and scanning electron microscopy (SEM), respectively. When mixed with sodium citrate, HM-CHI rapidly transformed human blood into an elastic gel. In contrast, un-modified chitosan (i.e. without hydrophobes) was unable to clot blood. The hydrophobias within HM-CHI entered into the RBC membranes and connected to the cells by a sample-spanning network, which subsequently led to the formation of an elastic gel. The gelling ability of HM-CHI is similar to that of fibrin-based sealants, but at a much lower cost and greater availability.
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高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Wei Xia,Xiao-Yi Wei,Yuan-Yuan Xie,Tao Zhou
A novel thioether chitosan oligosaccharide (COS-All-Tio) was prepared by the reaction of chitosan oligosaccharide (COS) with 3-bromopropene, followed by the coupling with tiopronin (Tio) using a thiol-ene reaction. The degree of substitution of COS-All-Tio reached 1.48. The structure of COS-All-Tio was identified by IR, NMR spectra . It was found that COS-All-Tio possessed more potent antioxidant activities than COS. The IC 50 values of COS-All-Tio for scavenging DPPH , ABTS + and OH were 0.31, 0.39 and 0.73 mg/mL, respectively, while the corresponding values for COS were 0.66, 2.89 and 1.41 mg/mL, respectively. COS-All-Tio was also found to possess much stronger antibacterial effect than COS against five bacteria strains ( Staphylococcus aureus , Bacillus subtilis , Listeria monocytogenes , Escherichia coli and Pseudomonas aeruginosa ). Further, COS-All-Tio was found to be non-toxic to RAW264.7 macrophages and MRC-5 human lung cells. This work provides a convenient way to improve the antioxidant and antibacterial activities of COS.
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DOI
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
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2016
·
IF 4.694
Wenjun Zhou,Lili Gao,Wentao Cheng,Lin Chen,Junfeng Wang,Hui Wang,Wei Zhang,Tianzhong Liu
Harvest of algal biomass from dilute culture medium at low cost remains a major hurdle to industrial scale processing. Conventional harvesting methods are not only costly, but also affect any later downstream processes partly because of the metal ions contamination by flocculants . This work constructed an electro-flotation technique including two steps of flocculation by chitosan first and then sequential bubbling flotation by non-sacrificial graphite electrodes . Laboratory work has been carried out to investigate the influences of chitosan dosage, pH value, cell density of algal culture, stirring rate and mixing time on flocculation efficiency, and the voltage and current intensity imposed on electrodes and the space between electrodes on flotation efficiency. Moreover, a 1000 L pilot bench of electro-flotator constructed to harvest Chlorella sp. was tested. Around 90% of total recovery efficiency and 50 times concentration rate have been reached, and only 23.7 g chitosan and 0.43 kWh of electricity are required to harvest 1 kg biomass. Above results proved electro-flotation by graphite electrodes assisted with chitosan is a safe and cost effective approach for microalgae harvesting.
研究领域:
环境工程
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生物技术
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微藻生物炼制
·
水处理
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DOI
高影响因子
Surfaces and Interfaces
·
2026
·
IF 6
Rubab Irshad,Muhammad Tahir Saddique,Shahid Nazir,Zaighum Tanveer
The carboxymethyl chitosan (CMCh) was synthesized and studied to carry out the process of wastewater treatment. The solubility of chitosan was enhanced by modifying its structural material with carboxymethyl chitosan. The present study was used to synthesize the novel adsorbent structure (CMCh/GLA/ECH) and its study was carried out by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Scanning electron microscopy (SEM) characterization. Previously the study was carried out on glutaraldehyde (CMCh/GLA), a single crosslinker. The latest research is on dual crosslinker and its activity in removing metal ions from metal contaminated water. The CMCh was cross linked with glutaraldehyde (GLA) and epichlorohydrin (ECH). In this study, the single (CMCh/GLA) and dual cross-linked (CMCh/GLA/ECH) polymer adsorbents were used to study the adsorption capacity of the adsorbent. The metal ions removal efficiency for Co (Ⅱ) and Cu (Ⅱ) was studied. The results obtained were fitted to the Langmuir and Freundlich adsorption model. The Langmuir model showed monolayer adsorption on the homogeneous surface of the adsorbent. The cross-linked polymer (CMCh/GLA/ECH) showed the maximum efficiency of the adsorbent prepared. Download: Download high-res image (180KB) Download: Download full-size image
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Nanomaterials
·
2016
·Vol. 6
·期 7
·130
·
IF 2.69
Yinxue Zu,Jingran Bi,Huiping Yan,Haitao Wang,Yukun Song,Bei-Wei Zhu,Mingqian Tan
Fluorescent nanostructures (NSs) derived from polysaccharides have drawn great attention as novel fluorescent probes for potential bio-imaging applications. Herein, we reported a facile alkali-assisted hydrothermal method to fabricate polysaccharide NSs using starch and chitosan as raw materials. Transmission electron microscopy (TEM) demonstrated that the average particle sizes are 14 nm and 75 nm for starch and chitosan NSs, respectively. Fourier transform infrared (FT-IR) spectroscopy analysis showed that there are a large number of hydroxyl or amino groups on the surface of these polysaccharide-based NSs. Strong fluorescence with an excitation-dependent emission behaviour was observed under ultraviolet excitation. Interestingly, the photostability of the NSs was found to be superior to fluorescein and rhodamine B. The quantum yield of starch NSs could reach 11.12% under the excitation of 360 nm. The oxidative metal ions including Cu(II), Hg(II)and Fe(III) exhibited a quench effect on the fluorescence intensity of the prepared NSs. Both of the two kinds of the multicoloured NSs showed a maximum fluorescence intensity at pH 7, while the fluorescence intensity decreased dramatically when they were put in an either acidic or basic environment (at pH 3 or 11). The cytotoxicity study of starch NSs showed that low cell cytotoxicity and 80% viability was found after 24 h incubation, when their concentration was less than 10 mg/mL. The study also showed the possibility of using the multicoloured starch NSs for mouse melanoma cells and guppy fish imaging.Keywords:polysaccharide nanostructures;fluorescent materials;quantum yield;cell imaging;fish imaging
研究领域:
纳米技术
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生物医学工程
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材料科学
·
生物学
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DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 121
·100114-100122
·
IF 3.84
Lingling Lei,Zongze He,Huanle Chen,David Julian McClements,Bin Li,Yan Li
Antimicrobial emulgels were fabricated by cross-linking chitosan using cinnamaldehyde (CA) nanoemulsions. The influence of system composition and pH on the properties of the emulgels was characterized using electron and confocal fluorescence microscopy, dynamic shear rheology, and Fourier transform infrared spectroscopy (FT-IR). FT-IR confirmed the occurrence of a Schiff base reaction between chitosan and CA. Rheology measurements helped establish the optimum pH and system composition required to fabricate emulgels. Microscopy provided information about the network structure formed within lyophilized emulgels. The emulgels were shown to have strong antibacterial activity against E. coli and S. aureus.
研究领域:
食品科学
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材料科学
·
微生物学
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高影响因子
CARBOHYDRATE POLYMERS
·
2011
·Vol. 85
·期 4
·832-837
·
IF 3.463
Dongke Zhao,Jun Huang,Sheng Hu,Jianwei Mao,Lehe Mei
Chitosan was prepared by alkaline N -deacetylation of squid cartilage β-chitin and carboxymethylated derivatives with different degrees of substitution (DS) were synthesized. The DS of the derivatives calculated by pH titration were 0.64, 0.81, 1.0, 1.33 and 1.59. The antioxidant properties and bile acid binding capacity of the derivatives were studied in vitro . The carboxymethylation of chitosan caused enhancement of bile acid binding capacity. At 1 mg/mL, carboxymethyl chitosan (CMCS) showed a stronger scavenging effect than chitosan towards DPPH radicals. The CMCS scavenging effect on superoxide radicals was stronger than that of chitosan, and EC 50 values were below 5.6 mg/mL. The effectiveness of reducing power correlated with the DS of CMCS. At 1.2 mg/mL, the ability of CMCS to chelate ferrous iron was 100%, whereas that of chitosan was only 22.34%. This suggested that carboxymethylation is a possible approach to obtain chitosan derivatives with desirable biological properties.
研究领域:
生物化学
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海洋生物技术
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食品科学
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高分子化学
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功能生物材料
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Yihao Song,Yaodong Ning,Xiaoqiong Shi,Zhanying Lu,Wenli Jiang,Yao Xu,Xinlu Zhang,Yingxin Qu,Xiaoling Lu
Seawater immersion wounds carry high health risks owing to seawater's high-salt, hypertonicity and diverse bacteria, and current therapies are limited and nonspecific, posing clinical challenges. In this study, high-viscosity chitosan (HVCS, viscosity >1000 mPa·s) from Antarctic krill shells was used as the matrix, glutamate as a molecular bridge linked dopamine and HVCS; high-viscosity dopamine-glutamate-chitosan (DGCS) was synthesized via amide bond-based covalent networks. DGCS inherits the rapid gel-forming ability of HVCS while showing its excellent favorable adaptability to seawater immersion wound surfaces. The introduction of dopamine and glutamic acid endows DGCS with more prominent mechanical properties and biological activities, including cell proliferation-promoting, antibacterial, and anti-inflammatory effects, and particularly inhibits the growth of specific Gram-negative pathogenic bacteria such as Vibrio vulnificus and Vibrio parahaemolyticus in vitro. More importantly, in seawater immersion wound rat models, DGCS hydrogel outperforms commercial chitosan dressings and silvadene in regulating wound inflammation, promoting collagen deposition, angiogenesis and healing. In conclusion, DGCS integrates antibacterial, anti-inflammatory and pro-healing functions, offering an effective multifunctional strategy for seawater immersion wound treatment, realizing the high-value-added valorization of shrimp shell waste and expanding HVCS's application in wound dressings.
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高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2011
·
IF 7.164
Congxin Li,Tianying Guo,Dezhong Zhou,Yuling Hu,Hao Zhou,Shufang Wang,Jiatong Chen,Zhengpu Zhang
A novel non-viral gene vector based on poly[poly(ethylene glycol) methacrylate] (PMPEG) and l -glutathione (GSH) grafted chitosan (CS) has been fabricated. First, well-defined brush-like PMPEG living polymers with dithioester residues were prepared by the reversible addition–fragmentation chain transfer (RAFT) polymerization and grafted onto the allylchitosan via radical coupling method. Then, the tripeptide GSH was introduced onto the end of PMPEG chain to give a CS–PMPEG–GSH conjugate. In comparison with pristine chitosan, CS–PMPEG–GSH conjugate could not only condense plasmid DNA (pDNA) and prevent the condensed CS–PMPEG–GSH/pDNA nanoparticle self-aggregation, but also increase the binding ability to cell membrane efficiently and improve decondensed ability of pDNA from the nanoparticles in cytoplasm which thus has resulted in the higher transfection efficiency in mouse embryonic fibroblast cells (NIH3T3). In addition, cytotoxicity assays showed that the conjugate is less cytotoxic than CS, and still retain the cationic polyelectrolyte characteristic as chitosan. These results indicate that the non-viral vector is a promising candidate for gene therapy in clinical application.
研究领域:
生物材料学
·
分子生物学
·
基因治疗
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产品关联
DOI
PubMed
高影响因子
SEPARATION SCIENCE AND TECHNOLOGY
·
2012
·
IF 1.088
Yu Yi,Canlin Lai,Yuqi Jiang,Wenyue Xiong,Jianfeng Mei,Guoqing Ying
A novel magnetic chitosan microsphere (MCM) was prepared from chitosan and Fe3O4 through inverse suspension crosslinking, epichlorohydrin activation, and grafted with ligands. The magnetometer measurements data indicated that the magnetic microspheres had superparamagnetic property as well as fast magnetic response without the magnetic hysteresis effect and thus can be well used in magnetic separation. The adsorption results showed that the magnetic chitosan microsphere grafted with ligand had good adsorption capacity on endotoxin up to 554.1 EU/g under the condition of 25.0 mg/mL adsorbent, 17.2 EU/mL endotoxin. It is expected that this new microsphere will have a feasible and useful application in adsorbing endotoxin from biological products.
研究领域:
生物医学工程
·
材料科学
·
生物技术
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Xu Liu,Yang Wang,Zheng Cheng,Jie Sheng,Rendang Yang
Bacterial cellulose is an attractive material due to its outstanding biocompatibility, nanometer size and high purity, but the difficulty of dispersing has restricted its wide applications. In this study, chitosan was grafted onto bacterial cellulose by Schiff base reaction, and then obtained the nano-sized fibrils by ultrasonic dispersion process. Different concentrations of NaIO 4 with various time and temperature were applied, and the maximum grafting amount of chitosan was 12.38%. The obtained products were characterized by FTIR, XRD, SEM and TEM. From the dispersibility and stability behavior research, the suspension containing chitosan was more uniform and showed better acid and temperature stability. It can also be observed in SEM and TEM images that the bacterial cellulose were dispersed into single ones and the diameter of the isolated fibril was 30–80 nm.
研究领域:
材料科学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2019
·Vol. 10
·期 18
·2305-2315
·
IF 4.76
Xiacong Zhang,Lin Cheng,Letian Feng,Yu Peng,Zhimin Zhou,Guoyong Yin,Wen Li,Afang Zhang
Stimulus-responsive injectable hydrogels with self-healing properties are promising materials for the delivery of bioactives owing to their protection of the payloads, minimally invasive procedures and site-specific administration. In this work, we report on a type of thermoresponsive dendronized chitosan-based (G1-CS) injectable hydrogel and its potential as scaffolds for stem cell delivery. G1-CS was synthesized through dendronization of chitosan with dendritic oligoethylene glycols (OEGs) to afford characteristic thermoresponsiveness, and its further crosslinking through dynamic covalent Schiff-base chemistry generated the hydrogels. The mechanical properties of the obtained hydrogels are weak with a storage modulus around 0.2 kPa at room temperature, which can be significantly improved to about 1.4 kPa at 37 °C. This feature comes remarkably from thermally induced aggregation and a simultaneously enhanced Schiff-base reaction in an aqueous phase, resulting in promoted crosslinking of the hydrogel network and immediate gelation under physiological conditions. The Schiff-base linkage also endows the hydrogels with shear-thinning and self-healing properties, which facilitate the injection process of the hydrogels and maintenance of their stability. G1-CS hydrogels were examined to encapsulate stem cells. After long term (14 days) culture, the cells exhibited high viability and well maintained their differentiation potential. We therefore believe that hydrogels from this dendronized chitosan, featuring biocompatibility, thermoresponsiveness, self-healing ability, and enhanced mechanical properties, can serve as promising scaffolds for drug delivery and tissue engineering.
研究领域:
材料科学
·
生物材料
·
组织工程
·
干细胞研究
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）