JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2022
·
IF 9.965
Chunchun Wang,Shijie Li,Mingjie Cai,Ruyu Yan,Kexin Dong,Junlei Zhang,Yanping Liu
Constructing novel Z-scheme heterojunctions is an effective strategy for obtaining high-performance photocatalysts. Herein, tetra (4-carboxyphenyl) porphyrin (TCPP) and graphene quantum dots (GQDs) were loaded on the surface of Bi 2 MoO 6 (BMO) to fabricate novel Z-scheme heterojunctions of TCPP/G/BMO. Especially, TCPP/G/BMO-2 showed an exceptional visible-light photoactivity for tetracycline (TC) degradation (81.0%, 40 min) and Cr(VI) reduction (90.7%, 60 min), respectively by 2.38 folds and 2.96 folds enhancement compared to sole Bi 2 MoO 6 . The substantial enhancement of activity is attributed to the synergy effect of the Z-scheme charge transfer mechanism and the improved light absorption. The degradation pathways of TC were inferred by determining the generated intermediates using high performance liquid chromatography-mass spectrometry (HPLC-MS), and the toxicity of the transformation products was assessed by Toxicity Estimation Software Tool (T.E.S.T). Overall, on the basis of trapping experiments and electron spin resonance spectra (ESR) analysis, the photocatalytic mechanisms of Cr(VI) reduction and TC degradation by the TCPP/G/BMO Z-scheme heterojunction was proposed. This work indicates TCPP/G/BMO could be a promising photocatalyst for wastewater treatment.
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Food Analytical Methods
·
2022
·Vol. 15
·期 8
·2069-2083
·
IF 3.498
Nanfack Carole D. V.,Yang Jia,Yuan Xiao,Sun Jiadi,Sun Xiulan,Ji Jian
Deoxyribonucleic acid (DNA) extraction, which is the mainstay of molecular amplification for food pathogen detection procedure, is dependent on laboratory infrastructure, cumbersome methods, and time consumption. To tackle these dependencies, we developed an enhanced mussel-inspired cellulose card for the convenient, eco-friendly, and rapid DNA extraction from bacteria present in food. This enhanced cellulose mussel-inspired DNA adhesive card (BDA card) was successfully prepared using Na + through the self-polymerization of biopolymer polyvalent ions and 3,4-dihydroxy-l-phenylalanine (L-Dopa). We observed that, when the biopolymer surface modification of the cellulose was NaCl-doped, the DNA extraction and purification performances of the obtained BDA card were enhanced when compared to the performance of the cellulose modified with the biopolymer doped with HCl. After the surface modification, the previously modified cellulose card was characterized with the Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The DNA extraction performances as well as the quality of the bacteria genomic DNA extracted were equally measured. The Na + -doped DNA adhesive cellulose card (BDA card) depicted the best bacteria genomic DNA extraction performances. When compared to commercially available bacteria genomic DNA extraction kits, the BDA card portrayed better DNA extraction performances, in a one-step extraction protocol. It allowed easy and convenient DNA extraction. The whole genomic DNA extraction process could be completed in 10 min. The concentration of the genomic DNA extracted from overnight bacteria culture was highest when extracted with the BDA card 459.39 ± 86 ng/µl as compared to Ezup and PureLink genomic DNA kits. When tested with spiked chicken, it was statistically equal to the Mag-MK food genomic DNA extraction kit. The proposed method provides a simple, efficient, eco-friendly, and convenient approach for bacteria genomic DNA extraction and purification from samples.
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FOOD CONTROL
·
2022
·
IF 6.652
Yening Qiao,Jinfeng Bi,Qinqin Chen,Xinye Wu,Xinwen Jin,Min Gou,Xinrui Yang,Giorgia Purcaro
Baked red jujube is a common food ingredient used to enrich the nutritional value and enhance the aroma. DDMP (2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one) with intense bitterness and potential toxicity may be present in several baked red jujube-based products but there are no well-defined methods to measure it quickly, sensitively, and accurately. Headspace solid-phase microextraction with gas chromatography-tandem mass spectrometry (HS-SPME-GC-MS/MS) was applied for the analysis of DDMP. The standard curve was linear between the concentrations of 0.01 and 100 μg/mL, with a limit of detection at 0.1 ng/g that below the sensory threshold of DDMP (2.06 μg/g). Five cultivars of baked red jujubes from China were evaluated with the method for DDMP content (4.55–46.50 μg/g). The results indicated that jujube cultivars with a higher molar ratio of proline to glucose, lower moisture content, and stronger ability to resist water stress should be avoided from baking to modulate the formation of DDMP.
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Food Packaging and Shelf Life
·
2021
·
IF 6.429
Lijing Lu,Yingjie Su,Jing Xu,Haoyue Ning,Xueyu cheng,Lixin Lu
In order to inhibit the spoilage of the packaged food , a gas phase controlled-release low-density polyethylene (LDPE) film containing carvacrol loaded with halloysite nanotubes (HNTs) was prepared and studied in this work. HNTs were etched by 4 mol/L hydrochloric acid to obtain HNT-4M, which increased the carvacrol loading capacity. Then, it was characterized before and after loading with carvacrol by transmission electron microscopy , nitrogen adsorption isotherms, and thermogravimetric analysis . A new LDPE film containing carvacrol adsorbed on HNT-4M was prepared by extrusion, and the physical properties were studied. In addition, the release rate of carvacrol in the film was measured, and the antibacterial and antioxidant properties of the film were evaluated. The results showed that the carvacrol load of HNT increased from 6.7% to 24.2% after acid etching. The diffusion coefficient of carvacrol from the active film containing HNT-4 M/carvacrol was 9.546 × 10 -12 cm 2 ·s -1 , which was decreased by 57.3% compared with the films directly adding carvacrol. Moreover, according to the DPPH radical scavenging assay and micro-atmosphere diffusion antibacterial test, it was verified that carvacrol retained its antioxidant activity and antibacterial activity in the new film.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Lei Su,Yifan Li,Zhenkai Wang,Yao-Yin Lou,Qi-Zheng Zheng,Zhangxiong Wu,Sheng-Peng Sun
Iron-based catalysts are widely used in peroxysulfate-based AOPs but suffering from low catalytic performance at neutral pH conditions, which limits the application in groundwater remediation. Herein, this study investigated the efficacy, mechanism and long-term durability of Fe-doped nitrogen carbon (Fe@NC) armor catalysts toward peroxysulfate activation for the degradation of anthropogenic phenolics (APs) in groundwater matrices. The results showed that nonradical activation of Oxone (i.e., HSO 5 – ) was obtained over the as-prepared Fe-3 % @NC-700 catalyst, high-valent Fe species ( Fe(IV) O) and 1 O 2 were identified as the dominant reactive species for the efficient degradation of APs by experiments and density functional theory (DFT) calculations. Notably, the DFT results indicated that HSO 5 – was favorably adsorbed on the Fe atoms owing to the electrostatic interaction, which transformed exothermically into SO 4 2– and 1 O 2 over Fe 3 C, accompanied by the generation of Fe(IV) O. Additionally, the Fe-3 % @NC-700 catalyst displayed long-term activity for in situ activation of Oxone in groundwater matrices without pH adjustment, resulting in ΔAPs: ΔOxone stoichiometry efficiencies at 4.2–5.0 % . Wheat seeds germination tests revealed that the biotoxicity of the treated water was reduced notably by the present catalytic system, which holds large potential application in treatment of APs-contaminated groundwater.
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BIOCHEMICAL PHARMACOLOGY
·
2021
·
IF 5.858
Xiaohua Lu,Ge Yan,Sabine M. Klauck,Edmond Fleischer,Anette Klinger,Yoshikazu Sugimoto,Letian Shan,Thomas Efferth
After decades of research, multidrug resistance (MDR) remains a huge challenge in cancer treatment. In this study, the cytotoxic of 4-hydroxy-N-(naphthalen-1-yl)-2-oxo-2H-chromene-3-carboxamide (MCC1734) has been investigated towards multidrug-resistant cancer cell lines. MCC1734 exerted cytotoxicity on cell lines expressing different mechanisms of drug resistance (P-glycoprotein, BCRP, ABCB5, EGFR, p53 knockout) to a different extent. Interestingly, sensitive CCRF-CEM cells and multidrug-resistant P-gp-overexpressing CEM/ADR5000 cells represented similar sensitivity towards MCC1734, indicating MCC1734 can bypass P-gp-mediated resistance. Microarray-based mRNA expression revealed that MCC1734 affected cells by multiple pathways, including cell cycle regulation, mitochondrial dysfunction, apoptosis signaling, and EIF2 signaling. MCC1734 stimulated the generation of excessive reactive oxygen species and the collapse of mitochondria membrane potential in CCRF-CEM cells, companied by the arrest of the cell cycle in the G 2 M phase and apoptosis induction as determined by flow cytometry. In addition, our immunoblotting analysis highlighted that MCC1734 triggered endoplasmic reticulum (ER) stress, evidenced by the activation of p-PERK, p-eIF2α, ATF4 and CHOP. The anti-cancer effects of MCC1734 were further observed in vivo using human xenograft tumors transplanted to zebrafish, providing further support for MCC1734 as a promising new candidate for cancer drug development.
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Chemistry Africa-A Journal of the Tunisian Chemical Society
·
2021
·Vol. 4
·期 2
·367-377
·
IF 0
Zou Miaomiao,Zou Suyan,Hu Chunyan,Lin Xiaoyun,Ni Yongnian
In this paper, graphite phase carbon nitride (g-C 3 N 4 ) was prepared by one-step high temperature thermal decomposition method, and layered g-C 3 N 4 was prepared by protonation with concentrated hydrochloric acid. The morphology and composition of g-C 3 N 4 were characterised by transmission electron microscope (TEM), energy dispersive spectroscopy (EDS) and X-ray powder diffraction (XRD). Furthermore, the AuNPs-g-C 3 N 4 /GCE was constructed by coating method and potentiostatic deposition method, and the construction process and resistance change of the electrodes were characterised by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The experimental results indicated that the AuNPs-g-C 3 N 4 /GCE has excellent electrocatalytic performance for bisphenol A (BPA) and 4-n-octylphenol (OP). Under optimized experimental conditions, BPA and OP were detected by differential pulse voltammetry (DPV) at the AuNPs-g-C 3 N 4 /GCE. The linear ranges were 0.1–6.5 µmol L −1 and 0.1–3.8 µmol L −1 for BPA and OP, wtih the detection limits of 0.0176 µmol L −1 and 0.0324 µmol L −1 , respectively. In addition, the AuNPs-g-C 3 N 4 /GCE showed satisfactory reproducibility and stability and can be successfully applied to monitor of BPA and OP in baby formula samples.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2020
·Vol. 21
·期 15
·5347
·
IF 4.556
Xiao-Ran Jing,Huan Liu,Yao Nie,Yan Xu
Fe(II)/2-ketoglutarate-dependent dioxygenase (Fe(II)/2-KG DO)-mediated hydroxylation is a critical type of C–H bond functionalization for synthesizing hydroxy amino acids used as pharmaceutical raw materials and precursors. However, DO activity requires 2-ketoglutarate (2-KG), lack of which reduces the efficiency of Fe(II)/2-KG DO-mediated hydroxylation. Here, we conducted multi-enzymatic syntheses of hydroxy amino acids. Using (2s,3r,4s)-4-hydroxyisoleucine (4-HIL) as a model product, we coupled regio- and stereo-selective hydroxylation ofl-Ile by the dioxygenase IDO with 2-KG generation from readily availablel-Glu byl-glutamate oxidase (LGOX) and catalase (CAT). In the one-pot system, H2O2significantly inhibited IDO activity and elevated Fe2+concentrations of severely repressed LGOX. A sequential cascade reaction was preferable to a single-step process as CAT in the former system hydrolyzed H2O2. We obtained 465 mM 4-HIL at 93% yield in the two-step system. Moreover, this process facilitated C–H hydroxylation of several hydrophobic aliphatic amino acids to produce hydroxy amino acids, and C–H sulfoxidation of sulfur-containingl-amino acids to yieldl-amino acid sulfoxides. Thus, we constructed an efficient cascade reaction to produce 4-HIL by providing prerequisite 2-KG from cheap and plentifull-Glu and developed a strategy for creating enzymatic systems catalyzing 2-KG-dependent reactions in sustainable bioprocesses that synthesize other functional compounds.Keywords:Fe(II)/2-ketoglutarate-dependent dioxygenase;2-ketoglutarate generation;regio- and stereo-selective synthesis;hydroxy amino acids;sequential cascade reaction
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NANO
·
2020
·
IF 1.566
Suping Wang,Yujiao Tu,Kunhao Qin,Xiaotian Yuan,Wei Chen,Yunlin Wei,Zhiwei Xu,Qi Zhang,Xiuling Ji
Phenolic compounds, especially 4-nitrophenol (4-NP), and chromium (VI) are highly toxic environmental pollutants. Thus, it is significantly important to establish a rapid and sensitive sensor for 4-NP and Cr(VI) monitoring in environment. In this paper, a novel approach has been adopted where E. coli -derived CDs (CDs- WT ) prepared by one step hydrothermal method previously and sensitized by ampicillin (turn-on) were used as fluorescence nanoprobe (CDs- WT -Amp) for 4-NP and Cr(VI) determination based on the inner filter effect quenching mechanism (turn-off). Then, the quenching fluorescence was reversed by addition of ampicillin (turn-on). The linear ranges of 4-NP and Cr(VI) detection were 0–50 μ M and 0–25 μ M, with the limits of detection 88.89 nM and 64.75 nM, respectively. The “turn-on-on-off-on” fluorescent nanosensor system was successfully used to determine Cr(VI) and 4-NP in water with satisfactory results. It shows that the “turn-on-off-on” nanoprobe has great promising potential for application to environmental pollutant detection.
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CYTOTECHNOLOGY
·
2021
·Vol. 73
·期 5
·715-726
·
IF 2.058
Xu Haiqiao,Lin Shibang,Huang Haizhou
CCL22, which could induce chondrocyte apoptosis, was identified to be overexpressed in damaged cartilage. This study was conducted with the aim of investigating the effects of CCL22 interference on chondrocyte injury. The osteoarthritis model was established by stimulating chondrocytes with LPS. The expressions of CCL22, CCR4, matrix metallopeptidase (MMP) 3, MMP9, MMP13, (a disintegrin and metalloproteinase with thrombospondin-like motifs) ADAMTS-4, collagen II and inflammatory cytokines were measured using quantitative reverse transcription PCR (RT-qPCR) and western blot. Besides, immunoprecipitation (IP) was employed to verify the binding of CCL22 and CCR4. After CCR4 was overexpressed, cell viability was observed using Cell Counting Kit-8 (CCK-8). Additionally, cell apoptosis as well as its related proteins was detected by TUNEL and western blot, respectively. ng What’s more, glycosaminoglycan (GAG) level was detected using GAG kits. CCL22 and CCR4 expression increased noticeably in LPS-stimulated ATDC5 chondrocytes. CCL22 inhibition could suppress the expression of CCR4 in LPS-induced ATDC5 cells. Likewise, CCL22 inhibition could revive the activation of LPS-induced ATDC5 cells by regulating CCR4. In addition, CCL22 knockdown alleviated inflammatory response and cell apoptosis through CCR4. Furthermore, the cartilage degradation of ADTC5 cells could be relieved by CCL22 silence via regulating CCR4. CCL22/CCR4 expression was increased in osteoarthritic cartilage injury and participated in the inflammation and cartilage degradation of chondrocytes.
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POLYMER DEGRADATION AND STABILITY
·
2020
·
IF 4.032
Yuehan Qian,Fuhao Dong,Lizhen Guo,Jiawen Guo,Hiba Shaghaleh,Yuqi Wang,Xu Xu,Shifa Wang,Shiwei Liu
Limonene is an important subsidiary agricultural product obtained from orange peel that can also be prepared by microbial engineering, but its application degree of deep processing is relatively lower. By studying the application of limonene to silicone rubber, the application field of limonene has been expanded. Triethoxy (2-(4-methylcyclohex-3-en-1-yl)propyl)silane (LTES) was synthesized by a hydrosilylation reaction in the presence of Karstedt's catalyst using limonene and triethoxysilane as raw materials. FT-IR and 1 H NMR were used to determine the structure of the product (LTES). Thereafter, a series of room temperature vulcanized (RTV) silicone rubbers was modified with the LTES cross-linking agent and hydroxy silicone oil under the catalytic action of dibutyltin dilaurate. The results showed that the tensile strength , elongation at break and heat resistance of the synthetic silicone rubber modified with LTES were significantly higher.
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JOURNAL OF MEMBRANE SCIENCE
·
2013
·
IF 4.093
Jiannan Ren,Shuling Zhang,Yang Liu,Yan Wang,Jinhui Pang,Qinhong Wang,Guibin Wang
A series of novel organic–inorganic hybrid proton exchange membranes were prepared from sulfonated poly(arylene ether sulfone) (Am-SPAES) with 4-amino-phenyl pendant group, (3-isocyanatopropyl)triethoxysilane (ICPTES) and tetraethoxysilane (TEOS) using a sol–gel method. The reactive ICPTES was used to react with the amino pendant groups of Am-SPAES to form covalent bonds between Am-SPAES and SiO 2 , which could effectively improve the compatibility between the polymer matrix and the inorganic SiO 2 phase. The obtained hybrid membranes exhibited excellent thermal and oxidative stability. The mechanical properties of the membranes were enhanced and the swelling ratio of the membranes was reduced by introducing the cross-linking network structure. Especially, these hybrid membranes possessed higher water uptake and proton conductivities compared to the pristine Am-SPAES membrane when the SiO 2 content was 3 and 6 wt%, whereas the same properties of the obtained hybrid membranes decreased when the SiO 2 content was 10 wt%. All of the membranes showed much lower methanol permeability than Nafion 117 did. In addition, the dispersion of the inorganic particles (SiO 2 ) in the Am-SPAES matrix could be improved by the strong covalent interactions between Am-SPAES and SiO 2 .
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危险品化学品经营许可证（带存储）