JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2026
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IF 6.7
Zhang Mei,Yu Bingzhi,Wang Qiuyu,Wang Jing,Shao Luze,Sun Qinqin,Zhou Jingqing,Chen Shuxin,Lu Xinyang,Yang Kun,Hou Jie,Lin Daohui
Household cooking reshapes antibiotic exposure from fish
not merely
by reducing parent residues, but by redistributing and transforming
mixtures ultimately consumed. Here, 60 antibiotics were surveyed in
10 commercial aquatic products, and snakehead fish ( Channa argus ) was selected as a model to compare
boiling, steaming, and air frying. All treatments reduced parent antibiotic
residues in muscle, with boiling yielding the lowest retention (18.42
± 0.41%). During boiling, muscle-associated parent antibiotics
decreased by 83.38 ± 1.04%, but only 13.70 ± 2.53% of this
loss was recovered in broth. Boiling also caused the greatest loss
of immobilized water and tissue fragmentation, while moist heating
generated a tightly associated water fraction during tissue restructuring.
Nontarget screening tentatively assigned 15 enrofloxacin transformation
products and revealed treatment-dependent profiles and prediction-weighted
signals. These findings indicate that parent-compound depletion alone
may overlook cooking-induced partitioning, broth coconsumption, and
putative transformation products.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2026
·
IF 4.8
Kai Wang,Li-Li Zhao,Lu-Ping Tang,You-Zhen Feng,Zhong-Yuan Cheng
Diabetic nephropathy (DN) represents a major complication of diabetes, where the early detection of renal dysfunction remains challenging due to the lack of sensitive and non-invasive biomarkers. This study reports the rational design and comprehensive evaluation of GGT-SO3, a novel activatable near-infrared fluorescent and photoacoustic probe, for the specific sensing of γ-glutamyl transpeptidase (GGT), an enzyme implicated in oxidative stress and disease progression. The probe demonstrated exceptional selectivity, low cytotoxicity, and a favorable dual hepatic-renal clearance profile. In a streptozotocin (STZ)-induced murine model of progressive diabetes, in vivo photoacoustic imaging revealed a clear, stage-dependent enhancement of renal GGT-SO3 signal, which correlated with histological evidence of kidney damage. Importantly, a strong correlation was also established between disease severity and the fluorescence intensity of the activated probe metabolite excreted in urine. These findings collectively highlight GGT-SO3 as a promising tool for the non-invasive visualization and longitudinal monitoring of renal GGT activity, offering a potential new strategy for assessing diabetic nephropathy progression.
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FOOD MICROBIOLOGY
·
2026
·
IF 5.4
Xinyue Hu,Xianghong Deng,Zhanrui Huang,Wanying He,Lianhua Hu,Guoqing Wu,Xiaojie Zhou,Hao Chen
This study revealed the effects and underlying mechanisms of key amino acids in marinade on A. parasiticus growth and aflatoxin B 1 (AFB 1 ) biosynthesis. Six key amino acids (with a concentration gradient of 0.5-8 g/L) from the marinade were selected and cultured them synchronously with A. parasiticus in this study. Solid/liquid culture combined with LC-MS/MS technology was used to analyse the effects of amino acids on colony growth, spore production, mycelial dry weight, and AFB 1 biosynthesis in A. parasiticus , and metabolomics was employed to elucidate the regulatory mechanisms of key amino acids. Results demonstrated that arginine, glycine, and lysine exhibited varying degrees of inhibitory effects. Specifically, 8 g/L lysine completely inhibited AFB 1 biosynthesis and significantly suppressed the mycelial growth. Proline, phenylalanine, and leucine remarkably promoted both the growth of A. parasiticus and AFB 1 production, with AFB 1 content reaching 324.50 ng/mL in the 8 g/L proline group, 3.45 times higher than in the control group. Metabolomic analysis indicated that proline synergistically enhanced AFB 1 biosynthesis by activating metabolic pathways, including the tricarboxylic acid (TCA) cycle and gibberellin metabolism. In contrast, lysine disrupted glucose metabolism and the TCA cycle, destroyed cell membrane integrity and inhibited A. parasiticus growth and toxin production at multiple levels. This study clarified the bidirectional regulatory effects and mechanisms of key amino acids in marinade, providing crucial technical references and theoretical foundations for controlling AFB 1 contamination in the production of marinated products by regulating marinade amino acid composition.
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INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
·
2026
·
IF 4.3
Zhen Yang,Haoyu Tan,Xiaoqing Yang,Yuling Zhang,Junhao Liang,Wenqing Kuang,Zhanfeng Zhang,Tong-Xian Liu,Xiangfeng Jing
Cholesterol homeostasis is crucial for cellular function in insects and other animals. However, the function of lipases in maintaining cholesterol homeostasis in insects was rarely investigated. In this study, we aimed to identify cholesterol-metabolizing lipases in the gut and explore their function in cholesterol regulation, using Helicoverpa armigera as a model organism. We identified 24 lipase genes containing the PF04083 domain in the H. armigera genome, four of which exhibited gut-enriched expression. Functional assays revealed that all four recombinant lipases hydrolyzed cholesterol esters, with two also capable of processing triacylglycerols (TAG). Molecular docking analyses further supported substrate specificity among these lipases. Single-gene knockout of HaOG200589 resulted in compensatory upregulation of two other lipases and cholesterol acyltransferase ( ACAT ). In contrast, simultaneous knockout of HaOG200551 , HaOG200552 , HaOG200553 , and HaOG200589 led to significant downregulation of the genes coding for Niemann–Pick Type C1b ( NPC1b ) and ACAT . Interestingly, intestinal free cholesterol level remained relatively stable despite these gene disruption events. These findings highlight the essential role of gut-localized lipases in insect sterol metabolism.
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ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2026
·
IF 12.2
Yunpeng Xue,Lu Li,Yuren Wang,Yuchen Xiong,Min Deng,Kang Song
Abstract Lake biofilms are microbial habitats for nitrogen transformation, but how carrier type and elevation influence N2O dynamics remains unclear. We examined abiotic- and biotic-carrier biofilms from 12 lakes spanning 4–4272 m using anoxic incubations, 15N tracing, selective inhibition, isotopocule analysis, and metagenomics. Net N2O emission potential increased with elevation in both carrier types, with the largest carrier contrast at high elevation, where biotic-carrier biofilms showed the highest net N2O emission potential. 15N partitioning showed a marked shift in emission sources. At low elevation, heterotrophic nitrification dominated abiotic-carrier biofilms (∼91%), whereas denitrification contributed ∼57% in biotic-carrier biofilms. At high elevation, denitrification accounted for 67–97% of net N2O emission across both carriers, and bacterial denitrification dominated the total denitrification rate potential. Metagenomic profiles provided mechanistic support: high-elevation biotic-carrier biofilms showed higher relative representation of genes for dissimilatory nitrate reduction to ammonium and nitrogen fixation, but had few nosZ-carrying genomes, indicating weak terminal N2O reduction capacity. Overall, carrier type and elevation jointly shape nitrogen transformation pathways. High-elevation lake ecosystems, particularly those with abundant biotic substrates, may harbor disproportionately important biofilm N2O hotspots under global change.
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Journal of Building Engineering
·
2026
·
IF 8.1
Jinzhu Tang,Tingyun Zheng,Yuan Jin,Zhaoyang Sun,Guoxing Sun
PCE lowers yield stress and viscosity of IPC, with stronger effects at higher dosages. • PCE adsorbs on particles first, forming a barrier that limits PAM adsorption and bridging. • PCE reduces PAM thickening by promoting coiled chains and disrupting PAM associations. In-situ polymerization is a promising strategy for enhancing both the mechanical performance and durability of cementitious materials. However, rapid polymerization at early age causes severe flowability loss, adversely affecting construction processes such as pumping and casting. Herein, we explore the possibility of improving the fluidity of in-situ polymerization modified cement paste (IPC) by incorporating polycarboxylate ether (PCE) superplasticizer, with emphasis on elucidating their interaction mechanisms. Rheological tests reveal that adding 0.5% PCE remarkably reduces the dynamic yield stress by 64% at 30 min, validating its contributing role in mitigating workability loss. Mechanistic experiments uncover the dual roles of PCE: 1) prior to the occurrence of in-situ polymerization, PCE molecules occupy the adsorption sites on particle surfaces to form a blocking layer, reducing PAM adsorption from 5.1 to 1.2 mg/g at 30 min and preventing interparticle bridging; and 2) the existence of PCE molecules in the interstitial solution drives the non-adsorbed PAM to adopt more coiled configurations by competitive solvation and disrupts PAM associations by breaking ion-mediated interactions, both of which undermine the thickening effect of PAM. Furthermore, the flexural strength of PCE-containing composites at 28 d reaches 14 MPa, remaining comparable to that of PCE-free counterparts. Our work provides a feasible route to mitigate workability loss in IPC, and the discovery in this work offers fundamental insights into the interaction mechanisms between PCE and in-situ polymerized polymers.
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ENVIRONMENTAL POLLUTION
·
2026
·
IF 7.2
Wenjie Zhang,Jiale Chen,Qi Wang,Qi Jing,Meiling Yang,Anping Ma,Banghua Wu,Yiru Qin
6PPDQ exposure induces oxidative stress-driven cytotoxicity in RAW 264.7 macrophages. • Multi-omics identifies glycerophospholipid metabolism as a key disturbed pathway. • LPA accumulation and Gpat3/Lpar2 upregulation link lipid alteration to inflammation. • 6PPDQ activates NF-κB signaling and elevates TNF-α, IL-6, and iNOS expression. • NAC pretreatment attenuates ROS, lipid alteration, and inflammatory responses. N -(1,3-dimethylbutyl)- N′ -phenyl- p -phenylenediamine quinone (6PPDQ), a transformation product of the tire antioxidant 6PPD, is an emerging environmental contaminant with increasing toxicological concern. While macrophages are critical in recognizing and managing chemical stress, how 6PPDQ exposure perturbs their innate immune functions and intracellular metabolic homeostasis remains largely unexplored. Here, RAW 264.7 macrophages were exposed to 6PPDQ and examined using biochemical assays, integrated transcriptomic and metabolomic analyses, and N -acetylcysteine (NAC)-based antioxidant intervention. Exposure to 6PPDQ caused dose-dependent cellular injury, characterized by increased membrane damage, excessive reactive oxygen species (ROS) accumulation, glutathione depletion, inflammatory mediator upregulation, mitochondrial dysfunction, and enhanced apoptosis. Multi-omics analysis further revealed significant alterations in glycerophospholipid-related metabolic profiles, accompanied by enrichment of TNF and NF-κB inflammatory signaling. Several phospholipid species levels were decreased, whereas the level of lysophosphatidic acid (LPA) and the expression of its related genes Gpat3 and Lpar2 were markedly elevated. Pretreatment with NAC not only suppressed ROS accumulation, but also attenuated both lipid metabolic and inflammatory alterations induced by 6PPDQ. Collectively, these findings suggest that oxidative stress is closely associated with alterations in glycerophospholipid-related metabolic profiles and inflammatory responses during 6PPDQ-induced macrophage injury. Download: Download high-res image (192KB) Download: Download full-size image
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FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Mubai Sun,Ni Yang,Yuguang He,Xinyu Miao,Honghong Niu,Mei Hua,Jinghui Wang,Da Li
Fermentation and post-ripening plays a significant role in shaping the nutritional value, taste, and aroma of natto. This study aimed to analyse nutritional trends in natto during fermentation and ripening, identify its characteristic volatile organic compounds (VOCs), and elucidate their formation pathways. VOCs were detected using HS-GC-IMS combined with sensory evaluation in the study. Dynamic changes in key nutritional components, protein secondary structure, and texture characteristics were also examined and correlated. During the fermentation phase observed every 4 h till 20 h, peptide and amino acid content increased due to proteolysis, accompanied by a continuous decrease in hardness, springiness, and chewiness, and an increase in adhesion. During the post-ripening stage over 20 h, the overall nutrient composition and textural properties remained relatively stable, except hardness, which continued to decrease. The study identified 41 VOCs and based on the calculated relative odour activity value (ROAV), 7 compounds had ROAVs above 1 throughout the process. Moreover, compared to the fermentation stage, the concentrations of 2,3-dimethylpyrazine and 2-ethyl-3-methylpyrazine were significantly increased ( p < 0.05) in the post-ripening. Furthermore, the study revealed that the distinctive aroma compounds in natto, which were significantly correlated with the contents of Phe, Tyr, Val, Ile, and Thr, included butyl acetate, 2-methoxy-3-methylpyrazine, 2-pentylfuran, ethyl propanoate, and 2,3-butanedione ( p < 0.05). In conclusion, this study provides valuable insights into the formation of diverse aroma compounds in natto and lays a theoretical foundation for producing high-quality natto with enhanced nutritional value, a soft texture and a rich, mellow aroma.
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Biomaterials Science
·
2025
·
IF 5.7
Yun Tang,Jinghan Lin,Tingting Zhao,Lina Sun,Tingjiao Liu
Clinical trials have proven the beneficial effect of biodegradable orthopedic composites on bone repair; however, the mechanisms underlying composite-induced bone formation have not yet been fully explored. AZ91D magnesium alloy coated with carbon nanotubes (CNTs) and calcium phosphate (CaP)/chitosan (CS) (CNTs-CaP/CS-AZ91D) is a synthetic composite consisting of magnesium alloy, CNTs and CaP/CS. Its extracts induce the production of the calcitonin gene-related peptide (CGRP), a local neuropeptide that contributes to osteogenic differentiation, in dorsal root ganglion (DRG) neurons. This study was aimed at validating the upregulation of CGRP in CNTs-CaP/CS-AZ91D extract-induced DRG neurons. Besides, bone marrow-derived mesenchymal stem cells (BMSCs) showed greater osteogenic capacity after co-culture with CNTs-CaP/CS-AZ91D extract-induced DRG neurons, and this co-culture promoted autophagy in BMSCs to facilitate osteogenic differentiation. The dysregulation of CREB1/TRIM16/JAK1/STAT3 signaling was determined in BMSCs co-cultured with CNTs-CaP/CS-AZ91D extract-induced DRG neurons. However, all these results were counteracted by the knockdown of the CGRP receptor. In vivo study showed accelerated osteogenesis in the femur of CNTs-CaP/CS-AZ91D-implanted rats; however, this effect was inhibited by the CGRP receptor antagonist BIBN4096BS. In summary, this study highlights the critical role of the peripheral nervous system in osteogenesis and suggests the potential of CNTs-CaP/CS-AZ91D for improving bone formation in the future.
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INORGANIC CHEMISTRY
·
2025
·
IF 4.7
Qingyuan Liu,Miao Zhang,Chenxin Sun,Chunli Xu,Baoxin Li
Currently, the chiral ligand-directed growth strategy has been predominantly employed for preparing chiral gold nanoparticles (AuNPs). In this work, we report the unexpected discovery of homochiral AuNP formation under cryogenic conditions (0 °C) without chiral ligand induction. Systematic investigations revealed significant temperature-dependent evolution of nanoparticle morphology and chiroptical properties across a broad thermal range (−10 to 75 °C). Notably, AuNPs synthesized at 0 °C exhibited a 4.2-fold enhancement in the extinction dissymmetry factor (g-factor) compared to those prepared at 60 °C. We propose a temperature-modulated crystallization mechanism governing chiral symmetry breaking during nanoparticle growth. The 0 °C-synthesized chiral AuNPs demonstrated exceptional enantioselective recognition capabilities, particularly as colorimetric probes for glutamine (Gln) enantiomer discrimination. This work developed a chiral ligand-free synthesis method for chiral AuNPs. Our findings establish temperature engineering as a crucial parameter for controlling nanoscale chirality and open new avenues for designing chiral plasmonic materials.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Jinxin Yu,Jiayi He,Xin Gong,Jimin Xu,Yongqiang Bai,Yiqiang Tian,Xilian Yang,Xinnian Zeng,Jiali Liu
Associative learning is central to studies of cognitive behavior in invertebrates, including insects. It involves acquiring new values from rewards and punishments by continuously updating sensory cues. Different biogenic amines guide the processes of reward and punishment. The role of dopamine (DA) signaling in associative learning has long been a focal point in the study of insect cognition. Previous studies across multiple insect species have established a general framework in which DA is primarily associated with aversive learning and punishment processing. However, emerging evidence suggests that DA has also been implicated in appetitive memory in certain species, indicating potential variability in the neuromodulatory mechanisms underlying associative learning. Here, we conducted behavioral assays, pharmacological interventions, and molecular manipulations to investigate the role of DA signaling in both aversive and appetitive classical conditioning in Bactrocera dorsalis , an economically important agricultural pest with well-documented learning and memory capabilities. We found that pharmacological blockade of DA signaling impaired aversive but not appetitive learning and memory, consistent with its conserved role in punishment processing. RNAi knockdown of DA receptors impaired both aversive learning and memory, reinforcing the pharmacological findings. Surprisingly, BdDOP2 knockdown impaired appetitive memory, uncovering a unique role for DA receptor subtype in appetitive memory. These findings provide new insights into the conserved and divergent functions of DA in insect cognition. Our results highlight B. dorsalis as a promising model for studying the neural mechanisms underlying associative learning, with potential applications in developing targeted pest control strategies by disrupting specific aminergic pathways involved in olfactory-based decision-making.
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Journal of Functional Foods
·
2025
·
IF 4
Yan-Ping Li,Tian Liang,Yan-Bin Hao,Jian-Lin Shen,Qi Zou,Jing Huang,Jiang-Hong Luo,Er-Sheng Gong,Xiao-Yin Wang
This study aimed at investigating the effects of lotus seedpod polysaccharide (LSP) against human gastric cancer AGS cells and N -methyl-N′-nitro-N-nitrosoguanidine (MNNG)-induced gastric cancer rats. Moreover, the mechanism of LSP against AGS cells was explored by transcriptome and western blot analyses. Results indicated that LSP inhibited proliferation, migration and invasion of AGS cells, induced cell cycle arrest and apoptosis, increased levels of Ca 2+ , ROS and TGF-β1, and decreased ΔΨ m and VEGF level. Moreover, LSP changed genes of AGS cells, especially downregulated the cell cycle pathway. CDC6, FZR1, CDC20, CDC27, MAD2L1, CDK1, CCNA2, ESPL1 and BUB1B were the hub targets for this pathway. LSP upregulated the protein expressions of FZR1 and CDC20, and downregulated the residual targets. Furthermore, LSP alleviated MNNG-induced gastric cancer in rats, and increased the levels of IgA and GSH in stomach. These findings indicate LSP possessed potential anti-gastric cancer activities, which supports the potential adjuvant application of LSP.
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