INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yiqing Chen,Yingbin Lv,Wei Su,Guangyu Wu,Peiyuan Li
Wound infections are a significant threat to human health. Therefore, the development of wound dressings with rapid antimicrobial properties is crucial to promote effective wound healing. In this study, chitosan (CS) was combined with gelatin (GE) to create an active film dressing (GC/BP) loaded with berberine hydrochloride (BH) and polypyrrole (PPY). After the incorporation of the bioactive materials, the film retained good mechanical properties, allowing it to withstand changes in the external environment of the wound. The antimicrobial effect of the GC/BP film exceeded 99 % after brief exposure to near-infrared light. In addition, the GC/BP film demonstrated a strong antioxidant effect with a DPPH clearance rate of 92.54 % within 48 h. In vivo experiments revealed that GC/BP films could enhance angiogenesis by upregulating the expression of the growth factor CD31, reducing oxidative stress by downregulating TNF-α expression, and accelerating the formation of fibrous tissues to promote wound healing. Importantly, the GC/BP film exhibited no cytotoxicity or hemolysis and demonstrated good biocompatibility. In conclusion, the GC/BP film is a safe and effective wound dressing with a promising potential for promoting wound healing.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Guoquan Zhang,Shaowei Hu,Xiaoyu Zhu,Xiaoxin Hu,Fenglin Yang
An interlocking “ T -shape” structure was built by uniform filling β-PbO 2 crystalline inside the trace Ti 3+ self-doping TiO 2 nanotube arrays (TNAs) channels and depositing the cauliflower-like β-PbO 2 clusters on its upper surface through a facile current pulse deposition method. We elaborated the interfacial engineering strategy for generating compact oxide layers at TNAs bottoms and growing β-PbO 2 crystalline from the selectively conductive TNAs bottoms. Different characterization techniques and electrochemical analyses were conducted to characterize the morphology, structure, and electrochemical property of the β-PbO 2 partial filling/covering TNAs (β-PbO 2 /TNAs/Ti) materials. We also systematically investigated and elucidated the performance and mechanism of β-PbO 2 /TNAs/Ti anode activated peroxymonosulfate (PMS) system via berberine removal/mineralization, reactive oxygen species (ROS) identification/contribution, intermediates analysis, energy consumption and practical applicability estimation. Results illustrated that filling β-PbO 2 inside TNAs can efficiently improve the directional electron-transfer efficiency, oxygen evolution over-potential, tolerance to the varied water matrices and service lifetime of β-PbO 2 /TNAs/Ti anode activated PMS system. The ROS including • OH, S O 4 ∙ - and 1 O 2 that generated from PMS electro-activation are responsible for efficient berberine degradation through two pathways. This study will provide a general approach to construct the versatile tube-filled TNAs-based materials for wide applications in water/wastewater treatment.
✓ 已验证
DOI
高影响因子
Current Drug Delivery
·
2021
·Vol. 18
·期 7
·914-921
·
IF 2.565
Han Nana,Huang Xuemei,Tian Xuehao,Li Tong,Liu Xiaojing,Li Wen,Huo Su,Wu Qianwen,Gu Yuhao,Dai Ziqi,Xu Bing,Wang Penglong,Lei Haimin
Background: In the field of antibacterial, nanomaterials are favored by researchers because of their unique advantages. Medicinal plants, especially traditional Chinese medicine, are considered to be an important source of new chemicals with potential therapeutic effects, as well as
an important source for the discovery of new antibiotics. MRSA is endangering people's lives as a kind of multidrug-resistant Staphylococcus aureus, which is resistant to tetracycline, amoxicillin, norfloxacin and other first-line antibiotics. It is a hotspot to find good anti-drug-resistant
bacteriae, nature-originated nanomaterials with good biocompatibility. Objective: We reported the formation of phytochemical nanoparticles (NPs) by the self-assembly of berberine (BBR) and 3,4,5-methoxycinnamic acid (3,4,5-TCA) from Chinese herb medicine, which had good antibacterial
activity against MRSA. Methods and Results: We found that NPs had good antibacterial activity against MRSA; especially, its antibacterial activity was better than first-line amoxicillin, norfloxacin and its self-assembling precursors on MRSA. When the concentration reached 0.1 μmol/mL,
the inhibition rate of NPs reached 94.62%, which was higher than that of BBR and the other two antibiotics (p < 0.001). It was observed by Field-Emission Scanning Electron Microscopy (FESEM) that NPs could directly adhere to the bacterial surface, which might be an important aspect of the
antibacterial activity of NPs. Meanwhile, we further analyzed that the self-assembly was formed by hydrogen bonds and π-π stacking through Ultraviolet-Visible (UV-vis), Fourier Transform Infrared Spectroscopy (FTIR), hydrogen Nuclear Magnetic Spectrum (1H NMR), and powder X-ray Diffraction
(pXRD). NPs’ morphology was observed by FESEM and TEM. The particle size and surface charge were characterized by Dynamic Light Scattering (DLS); and the surface charge was -31.6 mv, which proved that the synthesized NPs were stable. Conclusion: We successfully constructed a naturally
self-assembled nanoparticle, originating from traditional Chinese medicine, which had a good antibacterial activity for MRSA. It is a promising way to obtain natural nanoparticles from medicinal plants and apply them to antibacterial therapy.
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
Zijun Zhou,Yinli Xu,Xiong Xiao,Tao Huang,Jikai Zhao,Yuting Huang,Guoxin Zhang,Bo Xing,Qiusheng Zhao,Zijun Cao,Zhishang Wang,Zongtao Yin,Liming Yu,Huishan Wang
Background and purpose Our previous clinical study showed that berberine could reduce the occurrence of postoperative atrial fibrillation (AF), but the underlying mechanism remains unknown. Additionally, the effect of SIRT6 in the pathogenesis of AF and whether the anti-arrhythmic effects of berberine are associated with SIRT6 regulation remain uncovered. This study aimed to investigate the role of SIRT6 in the occurrence of AF and the pharmacological effect of berberine on atrial remodeling and AF susceptibility with a focus on SIRT6-AMPK signaling. Methods Bioinformatics tools and human atrial sample analysis were conducted to investigate the potential mechanisms. Murine AF model was induced by angiotensin II (Ang II) infusion with or without the treatment of berberine and the adeno-associated virus carrying SIRT6 or SIRT6-specific short hairpin RNA. The protective effect and the underlying mechanisms were examined. Results NLRP3 inflammasome-mediated inflammatory signaling in atrial tissue was activated, and SIRT6 activity was inhibited in AF patients. Berberine dose-dependently improved atrial structural and electrical remodeling in Ang II-induced mice. Moreover, berberine enhanced atrial antioxidative stress capacity and reduced NLRP3 inflammasome activation. SIRT6 overexpression mimicked these actions. Berberine-mediated SIRT6-AMPK signaling activation was inhibited by SIRT6 knockdown, and berberine-mediated inhibition of NLRP3 inflammasome signaling was compromised. Conclusion These results demonstrate that activation of SIRT6-AMPK signaling protects against Ang II-induced atrial remodeling and AF susceptibility. Berberine prevents Ang II-induced atrial injury by inhibiting NLRP3 inflammasome activation through the SIRT6-AMPK signaling pathway. Its potential as a preventive or therapeutic agent warrants further clinical investigation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYTOTHERAPY RESEARCH
·
2026
·
IF 6.3
Zijun Cao,Jinfeng Duan,Shan Meng,Zijun Zhou,Yuting Huang,Xin Chen,Jikai Zhao,Tao Huang,Guoxin Zhang,Tong Su,Liming Yu,Zongtao Yin,Huishan Wang
Heart failure with preserved ejection fraction (HFpEF) and atrial fibrillation (AF) frequently coexist due to shared risk factors, yet optimal therapeutic interventions remain elusive. Berberine (BBR), a widely used isoquinoline alkaloid, has demonstrated potential anti-arrhythmic properties. However, its specific effects and regulatory mechanisms in HFpEF-related AF are not fully understood. The present study was designed to clarify the pathological characteristics of HFpEF-associated AF and further evaluate the therapeutic effects of BBR. In this study, we developed a high-fat diet plus Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME)-induced murine HFpEF model to investigate the impact of berberine on the pathogenesis of HFpEF-associated AF. Bioinformatics and in vivo analyses revealed a progressive increase in atrial endoplasmic reticulum (ER) stress and lipid metabolic dysregulation. The HFpEF model exhibited significant atrial structural and electrical remodeling, which was dose-dependently reversed by berberine treatment. Further investigation showed that berberine effectively preserved atrial lipid metabolism, reduced ER stress and atrial inflammation, and restored atrial AMP-activated protein kinase (AMPK) activity. These protective effects were abolished by Compound C treatment, yet replicated by metformin or 4-phenylbutyric acid administration. Notably, the beneficial effects of berberine were validated in an isoprenaline/palmitic acid (ISO/PA)-treated HL-1 cell model. Our study highlights the therapeutic potential of berberine in mitigating HFpEF-associated atrial ER stress, lipid accumulation, and inflammatory responses. By targeting and preserving atrial AMPK signaling, berberine offers a promising therapeutic approach to combat HFpEF-associated AF.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2022
·
IF 5.988
Jingliang Wu,Cuiping Qi,Hao Wang,Qing Wang,Jingui Sun,Jinping Dong,Guohua Yu,Zhiqin Gao,Bo Zhang,Guixiang Tian
Cancer-associated fibroblasts (CAFs) are a major component of the tumor microenvironment (TME). In hepatocellular carcinoma (HCC), quiescent hepatic stellate cells (HSCs) could be activated to become CAFs, which play a critical role in tumor progression and drug resistance. Therefore, recent efforts have been focused on combining anti-HSC and pro-apoptotic activities to improve anti-tumor efficacy of drugs. In this study, glycyrrhetinic acid and hyaluronic acid–modified liposomes (GA-HA-Lip) were prepared for co-delivery of curcumin (CUR) and berberine (BBR) for the treatment of HCC. Furthermore, we established the LX-2+BEL-7402 co-cultured cell model and implanted the m-HSCs+H22 cells into a mouse to evaluate the anti-tumor effect of CUR&BBR/GA-HA-Lip both in vitro and in vivo . The results showed that CUR&BBR/GA-HA-Lip could accumulate in tumor tissues and be taken up by HSCs and BEL-7402 cells simultaneously. Compared with free CUR, the combination therapy based on GA-HA-Lip exhibits stronger pro-apoptotic and anti-proliferation effect both in vitro and in vivo . The anti-tumor mechanistic study revealed that CUR&BBR/GA-HA-Lip could inhibit the activation of HSCs and restrain drug resistance of tumor cells. In summary, CUR&BBR/GA-HA-Lip could be a promising nano-sized formulation for anti-tumor therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Junpeng Sun,Xiaobang Liu,Jiaqun Du,Jinyu An,Yingqiao Li,Yu Hu,Shuai Cheng,Ying Xiong,Yanan Yu,He Tian,Xifan Mei,Chao Wu
In this study, manganese-doped albumin-gelatin composite nanogels (MAGN) were prepared and used to load berberine (Ber) for the treatment of gouty arthritis (GA). The nanodrug delivery system (Ber-MAGN) can target inflammatory joints due to the intrinsic high affinity of albumin for SPARC, which is overexpressed at the inflammatory site of GA. Characterization of the pharmaceutical properties in vitro showed that Ber-MAGN had good dispersion, and the particle size was 121 ± 10.7 nm. The sustained release effect significantly improved the bioavailability of berberine. In vitro and in vivo experimental results showed that Ber-MAGN has better therapeutic effects in relieving oxidative stress and suppressing inflammation. Therefore, Ber-MAGN, as a potential pharmaceutical preparation for GA, provides a new reference for the clinical treatment plan of GA.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Advances
·
2025
·
IF 6
Amirhossein Kiumarsi,Mohammad Reza Farahpour,Zohreh Ghazi Tabatabaei,Hamed Hamishehkar
Advanced and novel effective wound dressings that decrease wound infections are essential for clinical application. In this study, a multifunctional hydrogel was engineered based on polyvinyl alcohol (PVA), polydopamine (PDA), and porous silica (PSi), loaded with berberine chloride (Bbn), for the treatment of MRSA-infected wounds. The PVA@PSi/PDA-Bbn hydrogel was fabricated via sol-gel and self-polymerization methods, forming a porous, adhesive, and photothermal-responsive matrix. The hydrogel demonstrated excellent swelling behavior, mechanical strength, and sustained Bbn release under NIR irradiation. The efficacy of the hydrogels was evaluated through antibacterial activity, pathological assessments, and the expression of key proteins involved in cell proliferation and wound healing. These proteins included cyclin D1, c-Myc, Max, FGF-1, β-catenin, and WNT-1, and COL1A. The physicochemical properties of the hydrogels confirmed their successful synthesis and suitability for biomedical applications. The results for swelling, rheological properties, adhesive strength, safety, and antibacterial activity confirmed the hydrogels to be appropriate. The PVA@PSi40/PAD-Bbn hydrogel could enhance the healing process through its antibacterial activity and modulation of the expressions of cyclin D1, c-Myc, Max, FGF-1, β-catenin, and COL1-A. In sum, PVA@PSi40/PAD-Bbn could compete with the mupirocin ointment® and accelerate the MRSA-wound healing process. It showed better performance than conventional treatments by providing controlled drug release, strong tissue adhesion, and effective MRSA inhibition to promote wound healing.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2020
·Vol. 44
·期 6
·1220-1230
·
IF 2.878
Yuan Wang,Shi-Jun Yin,Cong-Peng Zhao,Guo-Ying Chen,Feng-Qing Yang
In this study, a zirconium terephthalate metal-organic framework-coated magnetic nanoparticle (UiO-66@PA@PEI@Fe 3 O 4 ) was synthesized for the extraction of berberine prior to high-performance liquid chromatography analysis. The phytic acid, which could be grafted onto the magnetic nanoparticle through electrostatic interaction with the abundant amino groups of polyethylenimine, possesses outstanding metal ion coordination ability for the immobilization of metal-organic frameworks UiO-66. The physicochemical properties of the obtained nanoparticle were thoroughly investigated by a series of characterization techniques. Then, the factors that will affect the extraction efficiency and recovery of berberine were investigated. Results indicated that the material had good stability and reusability, and high adsorption capacity (50.01 mg/g) to berberine through single-layer adsorption. In addition, a molecular docking study indicated that the interactions between the material and berberine were mainly π-π stacking and hydrophobic interaction. Finally, the material was successfully applied to the extraction of berberine in Rhizoma Coptidis and Cortex Phellodendri extracts with the recoveries of 76.1% and 71.6%, respectively.
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2018
·
IF 3.862
Jianmei Sun,He Bao,Yajie Peng,Haimin Zhang,Ya Sun,Jiajun Qi,Hailong Zhang,Yang Gao
This study aims to evaluate the effects of Gelucire44/14 on the in vitro transport, in situ intestinal absorption, as well as in vivo antidiabetic efficacy of berberine (BBR). In the in vitro study, Gelucire44/14 (0.1%, v/v) increased the absorptive transport of BBR across the intestinal membrane of a rat and reduced the relative transport in the secretory direction, thus demonstrating its potential inhibitory effect on intestinal P-glycoprotein (P-gp). In the in situ absorption study, Gelucire44/14 (0.1%, v/v) increased BBR absorption, and this enhancing effect was more significant in the ileum than in the colon of a rat. Oral delivery of BBR with Gelucire44/14 (0.1%, v/v) to diabetic mice, compared with the BBR group, induced a significant hypoglycemic effect on day 7 and day 12 after administration. This result was well correlated with the results of the in vitro study, indicating the important contribution of the P-gp inhibitory effect of Gelucire44/14 to the improvement of the antidiabetic efficacy in vivo . In addition, Gelucire44/14 (0.1%, v/v) neither increased the levels of protein and lactate dehydrogenase in intestinal perfusion nor changed the morphology of the rat intestinal epithelium relative to those of the negative control. This finding suggested that 0.1% (v/v) Gelucire44/14 caused no apparent membrane damage to rat intestine. In conclusion, Gelucire44/14 exhibited potential for enhancing the oral absorption of BBR, thereby improving the antidiabetic efficacy of BBR.
✓ 已验证
DOI
PubMed
高影响因子
AAPS PHARMSCITECH
·
2026
·Vol. 27
·期 4
·181
·
IF 5.1
Zhang Jiayu,Zhang Shengyu,Wang Chunxue,Cai Binxin,Yang Hongxuan,Zhuang Kai,Swarnakar Nitin,Tiwari Sandip,Zhou Feng,Wang Wenxi,Zhou Yang,Zhang Jiantao
Berberine hydrochloride (BH) is a naturally isoquinoline alkaloid extracted from Coptis species. It possesses an extremely intense bitter taste and is classified as a Biopharmaceutics Classification System (BCS) class III drug, exhibiting high aqueous solubility but limited intestinal permeability. Conventional taste-masking polymers, including Kollicoat® Smart Seal and Eudragit® EPO, are predominantly gastric-soluble. While effective in suppressing bitterness, their use may delay or reduce drug release in the intestine, potentially exacerbating the inherently low oral bioavailability of BH. To overcome this limitation, this study employed the enteric polymer Kollicoat® MAE 100–55 to develop an amorphous solid dispersion (ASD) designed to achieve taste masking without compromising intestinal drug release. Following formulation screening, an amorphous solid dispersion of berberine hydrochloride (BH-ASD) was successfully prepared via hot-melt extrusion. Simulated salivary dissolution and bitterness assessments demonstrated pronounced taste-masking performance, which was further enhanced by the inclusion of a minor amount of citric acid. Under non-sink conditions in pH 6.8 phosphate-buffered saline, the BH-ASD exhibited a moderately increased apparent solubility. Consistent with these findings, in vivo pharmacokinetic studies showed a 2.21-fold increase in oral bioavailability relative to crystalline BH. Overall, this study offers a promising formulation strategy to mitigate bitterness and enhance oral bioavailability. More importantly, this HME-based ASD platform presents a viable and scalable approach for the development of patient-centric oral dosage forms of BH. Graphical
The alternative text for this image may have been generated using AI.
✓ 已验证
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Jia-Man Liang,Fang Zhang,Yu-Lin Zhu,Xiao-Yan Deng,Xian-Ping Chen,Qiu-Ju Zhou,Ke-Jun Tan
Here, the high fluorescent silicon-doped carbon quantum dots (Si-CQDs) were prepared by a facile and one-pot hydrothermal assay using 3-aminopropyltrimethoxysilane as the carbon and silicon source. The prepared Si-CQDs exhibit favorable water-soluble, high-temperature resistance, acid resistance, alkali resistance, high ionic strength resistance, high photostability, film-forming ability and solid-state fluorescence. Compared to other Si-CQDs that have been reported, the prepared Si-CQDs show unique up-conversion fluorescence. Furthermore, it is found that berberine hydrochloride (BH) can effectively quench the down- and up-conversion fluorescence of the Si-CQDs, making it can be used as a highly sensitive and specific probe for BH dual-mode sensing. Meanwhile, the linear range of down-conversion fluorescence detection for BH is 0.5-30.0 µmol/L with a limit of detection (LOD) of 50 nmol/L, and the linear range of up-conversion fluorescence assay for BH is 0-25.0 µmol/L. The mechanism of down-conversion fluorescence quenching by BH was investigated through a series of studies. The results show the quenching mechanism is the inner filter effect (IFE). Moreover, this proposed strategy has been well used to analyze BH in urine samples with satisfactory results.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）