ANALYST
·
2018
·Vol. 143
·期 17
·4199-4205
·
IF 3.864
Jinzha Zhang,Yang Shen,Yuhong Liu,Zaiying Hou,Yuanqing Gu,Wenbo Zhao
A novel electrochemiluminescence (ECL) cytosensor was proposed for the quantitative detection of HeLa cells (human cervical cancer cells) with the help of a signal amplification strategy. Firstly, the Au-NaYF4:Yb,Er nanocomposites were prepared by a simple in situ hydrothermal method and characterized by transmission electron microscopy (TEM) images, X-ray diffraction (XRD) patterns, UV-vis spectra and Fourier transform infrared (FTIR) spectra. Compared with the bare NaYF4:Yb,Er nanocomposites, the ECL intensity of Au-NaYF4:Yb,Er nanocomposites was greatly enhanced by about 4.2-fold which can be attributed to the good conductivity of gold nanoparticles (Au NPs). The nanocomposites showed high and stable ECL emission, fast response and superior conductivity, all of which were advantageous to the ECL detection. Furthermore, HeLa cells were immobilized on the modified electrode via the interaction between folic acid and a folate receptor present on the cell surface. The ECL cytosensor showed satisfactory sensitive response to HeLa cells in a linear range of 4.25 × 102–4.25 × 105 cells per mL with a low detection limit of 326 cells per mL. The proposed cytosensor had good sensitivity and stability, which can offer a great potential platform for bioassay analysis.
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PubMed
高影响因子
Nanoscale
·
2017
·Vol. 10
·期 6
·2834-2840
·
IF 7.367
Bin Zhou,Bing Xu,Huimei He,Zhanjun Gu,Bing Tang,Ying Ma,Tianyou Zhai
We report the enhancement of green upconversion luminescence in tetrahedral LiYF4:Yb/Er nanoparticles by Mn2+ ion doping, which is different from the enhanced single-band red emission dominated by Mn2+ ions in cubic NaLnF4:Yb/Er (Ln = Y, Gd, Lu) nanoparticles. The energy levels of the first excited state 4T1 of Mn2+ in tetrahedral LiYF4:Gd and cubic NaGd(Y)F4 are compared by detection of emissions from Mn2+via the energy transition from Gd3+ to Mn2+ with excitation at 275 nm. The coordination environments of Mn2+ in these two host lattices have been investigated by X-ray absorption fine structure measurements. The results demonstrate that the formation of tetrahedral MnF4 in tetragonal LiYF4 arising from the replacement of Ln3+ ions with Mn2+ ions leads to a higher energy level of the Mn2+ 4T1 state than that in octahedral MnF6 in cubic NaYF4. The high-lying excited state of tetrahedral MnF4 is close to the green emitting 4S3/2 state of Er3+ and thus enhances green upconversion emission in tetragonal LiYF4:Yb/Er, while the low-lying excited state of octahedral MnF6 dominates red emission in cubic NaYF4:Yb/Er. These findings provide direct evidence for the key roles of the host lattices and more possibilities in modulating the upconversion behaviour of lanthanide-based nanoparticles by transition-metal ion doping to achieve the desired goals of specific applications.
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高影响因子
Journal of Materials Chemistry C
·
2022
·Vol. 10
·期 42
·15897-15905
·
IF 8.067
Hanchang Huang,Maohui Yuan,Zhongyang Xing,Wenda Cui,Tongcheng Yu,Shuai Hu,Guomin Zhao,Chuan Guo,Kai Han
In lanthanide-sensitized upconversion (UC) nanomaterials, the typical sensitizer Yb3+ can significantly modulate the codoped activator (such as Er3+ ions) to generate multiband transitions. However, the kinetics of these multiband emissions affected by the Yb3+ concentration remains unclear. In this work, we employ nanosecond time-resolved spectroscopy to investigate a single optically trapped NaYF4:Yb/Er microcrystal (MC) doped with different Yb3+ concentrations. Interestingly, high doping Yb3+ ions shorten the UC response time by a factor of 10 (from ∼1 μs to ∼100 ns) compared to low doping Yb3+. Meanwhile, 430 nm light (4G7/2 → 4I11/2) emitted before 654 nm (4F9/2 → 4I15/2) with Yb3+ dramatically increasing (opposite to the normal low Yb3+ doping cases), representing a new four-photon pathway (4I15/2 → 4I11/2 → 4F7/2 → 2H11/2 →4S3/2 → 2G7/2 → 4G11/2 → 2H9/2 → 2D5/2 → 4G7/2). Furthermore, we observed a broad range of time-dependent emission color, changing from being initially green over near-white to red. Particularly, the red emission is dominated in the decay process, yielding insight into the fundamental multiband UC kinetics. The result shows that varying the Yb3+ doping concentration provides a way to control the energy flow between the energy levels of Er3+ ions, which can modulate the response time and color evolution of UC on Yb/Er doped materials. This can direct the prospective development of fast-response optoelectronic devices, photocatalysis, and UC displays in the future.
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CRYSTENGCOMM
·
2020
·Vol. 22
·期 19
·3361-3370
·
IF 3.117
Yini Mao,Li Jiang,Pengfei Xian,Qiongfen Fu,Shanshan Hu,Jun Yang
A graphical abstract is available for this content
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SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Linlin Zhao,Juan Li,Yuhong Liu,Yuan Wei,Jin Zhang,Jinzha Zhang,Qing Xia,Qiang Zhang,Wenbo Zhao,Xiaoqiang Chen
We reported a novel ECL immunosensor for detection of carcinoembryonic antigen (CEA) based on the reduced graphene Oxide-BaYF 5 :Yb, Er (rGO-BaYF 5 :Yb, Er) nanocomposites. The rGO-BaYF 5 :Yb, Er nanocomposites, used as electrode luminescence materials, were prepared by in situ hydrothermal method, and characterized by TEM, XRD, UV–vis spectra, and upconversion fluorescence spectra. Further, poly (diallyldimethylammonium chloride) (PDDA), Au nanoparticles (AuNPs), and Anti-CEA were successively assembled on the surface of rGO-BaYF 5 :Yb, Er nanocomposites modified-electrode for fabricating ECL immunosensor. The results of the ECL measurements of CEA indicated that this novel ECL immunosensor exhibited high sensitive response to CEA in a linear range of 0.001–80 ng mL −1 with a low detection limit of 0.87 pg mL −1 , good selectivity and satisfactory stability.
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BIOMATERIALS
·
2015
·
IF 8.557
Leyong Zeng,Yuanwei Pan,Ying Tian,Xin Wang,Wenzhi Ren,Shouju Wang,Guangming Lu,Aiguo Wu
The combination therapy has exhibited important potential for the treatment of cancers, especially for drug-resistant cancers. In this report, bi-functional nanoprobes based on doxorubicin (DOX)-loaded NaYF 4 :Yb/Tm–TiO 2 inorganic photosensitizers (FA-NPs-DOX) were synthesized for in vivo near infrared (NIR)-triggered inorganic photodynamic therapy (PDT) and enhanced chemotherapy to overcome the multidrug resistance (MDR) in breast cancers. Using the up-conversion luminescence (UCL) performance of NaYF 4 :Yb/Tm converting near-infrared (NIR) into ultraviolent (UV) lights, reactive oxygen species (ROS) were triggered from TiO 2 inorganic photosensitizers for PDT under the irradiation of a 980 nm laser, by which the deep-penetration and low photo-damage could be reached. Moreover, nanocarrier delivery and folic acid (FA) targeting promoted the cellular uptake, and accelerated the release of DOX in drug-sensitive MCF-7 and resistant MCF-7/ADR cells. The toxicity assessment in vitro and in vivo revealed the good biocompatibility of the as-prepared FA-NPs-DOX nanocomposites . By the combination of enhanced chemotherapy and NIR-triggered inorganic PDT, the viability of MCF-7/ADR cells could decrease by 53.5%, and the inhibition rate of MCF-7/ADR tumors could increase up to 90.33%, compared with free DOX. Therefore, the MDR of breast cancers could be obviously overcome by enhanced chemotherapy and NIR-triggered inorganic PDT of FA-NPs-DOX nanocomposites under the excitation of a 980 nm laser.
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高影响因子
RSC Advances
·
2017
·Vol. 7
·期 43
·26633-26639
·
IF 3.108
Chenxi Song,Shubiao Zhang,Quan Zhou,Lei Shi,Linying Du,Defu Zhi,Yinan Zhao,Yuhong Zhen,Defeng Zhao
β-NaYF4:Yb,Er upconversion nanoparticles (UCNPs) were synthesized by a one-pot solvothermal method. The UCNPs were characterized through X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to prove that they possessed hexagonal crystal phase with uniform particle size distribution. The cationic solid lipid nanoparticles (CSLNs) were then obtained by coating the UCNPs with a lipid. Further analysis by FTIR, XRD and TEM indicated the lipid can successfully coat UCNPs, and the coating process had no obvious influences on morphology and crystallization of the UCNPs. Furthermore, the CSLNs were used for bioimaging in cells and delivering small interfering RNA (siRNA). Cellular bioimaging and upconversion luminescence studies clearly demonstrated that the CSLNs could effectively enter into cells with the help of the lipid. Silencing assays and cytotoxicity in A549 cells convincingly showed that the CSLNs could effectively deliver siRNA and possessed low cytotoxicity and good biocompatibility. Thus, the bifunctional CSLNs could be used as a potential candidate in bioimaging and therapeutic applications.
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Analytical Methods
·
2017
·Vol. 9
·期 22
·3296-3301
·
IF 1.9
Lei Wang,Yuan Ji,Yuezhen He,Lun Wang,Hongqi Chen
Li+-Doped NaYF4:Yb,Er upconversion luminescent nanoparticles (UCLNPs) were synthesized and modified with poly(acrylic acid) (PAA). The luminescence of the carboxyl-functionalized UCLNPs at 664 nm was quenched in the presence of cupric ions (Cu2+) due to the coordination of Cu2+ with the carboxyl group of PAA. When pyrophosphate ions (PPi) were added, the luminescence of the UCLNPs was restored due to the competitive binding of PPi with Cu2+. However, pyrophosphatase (PPase) could promote the hydrolysis of PPi, thus releasing Cu2+, leading to requenching of the luminescence. Under optimal conditions, the luminescence response was virtually proportional to the activity of the PPase between 0.500 and 50.0 mU mL−1, and the limit of detection was 0.441 mU mL−1. Based on the competition assay approach, a new highly sensitive method in the red region for real-time detection of PPase activity was developed.
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高影响因子
ACS Photonics
·
2024
·
IF 6.5
Wenxia Wu,Yazhao Wang,Limin Wu,Huiting Zhang,Zhonghua Zhu,Shengdi Ta,Peng Zhang,Shoulei Xu,Bernard A. Goodman,Wen Deng
Under excitation at 980 nm, Tm3+ may emit ultraviolet, blue, and red light, and Ho3+ may emit green light through multiphoton upconversion processes in Yb/Tm/Ho codoped materials. However, because the probability of the electron population of the higher excited state energy levels is much lower than that of the lower ones in multiphoton upconversion processes, it is difficult to observe the ultraviolet emission of Tm3+-doped single crystals under excitation at 980 nm. Here, we report that high-quality single crystals of cubic ZrO2 codoped with Tm2O3, Ho2O3, and Yb2O3 are successfully grown by the optical floating zone method, and ultraviolet emission at 308 nm was observed for the first time from multiphoton upconversion transitions in these highly transparent, low phonon energy single crystals under excitation at 980 nm. This result demonstrates the prospect of making ultraviolet solid-state lasers pumped by near-infrared light. Moreover, bright white light was emitted from the single crystal prepared with 5.00 mol % Yb2O3, 0.75 mol % Tm2O3, 0.0075 mol % Ho2O3, 2.2425 mol % Y2O3, and 92.00 mol % ZrO2 under excitation at 980 nm, and a bright solid white light source excited by a near-infrared light-emitting diode for practical use is presented.
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
·
IF 5.6
Cijun Shuai,Jie Zeng,Zhaoxiong Zhang,YouWen Yang,MingLi Yang,Hengyun Ye,Pan He,Jun Zan,Jun Zhang
Photosensitizer-mediated photodynamic therapy (PDT) enables wireless tumor inactivation by releasing cytotoxic reactive oxygen species (ROS). Nevertheless, the activation of conventional photosensitizers typically depends on visible light with limited tissue penetration, thereby restricting their therapeutic efficacy in deep-seated tumors. To overcome this limitation, an upconversion implant was engineered to function as a flexible internal light-emitting device by converting deeply penetrating near-infrared-I (NIR-I) light into visible light. Specifically, a composite PDT nanosystem was constructed by linking NaYF 4 :Yb,Er upconversion nanoparticles with the rose bengal photosensitizer—whose emission and absorption spectra are well-matched—using polyvinylpyrrolidone (PVP) as a molecular bridge. This PDT nanosystem was subsequently incorporated into poly-L-lactic acid (PLLA) scaffolds fabricated via selective laser sintering. Under 980 nm laser irradiation, the upconversion implant was capable of wirelessly emitting upconverted visible light through more than 2 cm of an in vitro tissue model and generating abundant ROS via the photodynamic effect. In vitro cell experiments demonstrated that the system effectively eliminated tumor cells by damaging the cell membrane and cellular genetic material. These results suggest that the scaffolds possess significant potential as anti-tumor tissue implants and offer a promising avenue for the development of innovative tumor treatment strategies.
研究领域:
生物医学工程
·
纳米技术
·
肿瘤学
·
光医学
·
材料科学
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高影响因子
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
·
2021
·
IF 5.302
He Junjie,He Guo,Liu Jing,Tao Jingchao
High-entropy ceramics exhibit great application potential as thermal barrier coating (TBC) materials. Herein, a series of novel high-entropy ceramics with RE 2 (Ce 0.2 Zr 0.2 Hf 0.2 Sn 0.2 Ti 0.2 ) 2 O 7 (RE 2 HE 2 O 7 , RE = Y, Ho, Er, or Yb) compositions were fabricated via a solid-state reaction. X-ray diffraction (XRD) and energy dispersive spectrometry (EDS) mapping analyses confirmed that RE 2 HE 2 O 7 formed a single defect fluorite structure with uniform elemental distribution. The thermophysical properties of the RE 2 HE 2 O 7 ceramics were investigated systematically. The results show that RE 2 HE 2 O 7 ceramics have excellent high-temperature phase stability, high thermal expansion coefficients (10.3–11.7 × 10 −6 K -1 , 1200 ℃), and low thermal conductivities (1.10-1.37 W m -1 K -1 , 25 ℃). In addition, RE 2 HE 2 O 7 ceramics have a high Vickers hardness (13.7–15.0 GPa) and relatively low fracture toughness (1.14-1.27 MPa m 0.5 ). The outstanding properties of the RE 2 HE 2 O 7 ceramics indicate that they could be candidates for the next generation of TBC materials.
研究领域:
材料科学
·
陶瓷
·
热工工程
·
高温材料
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高影响因子
CERAMICS INTERNATIONAL
·
2016
·
IF 2.758
Hao Li,Zonglian Huang,Lijin Cheng,Shu Kong,Shaojun Liu
Novel monoclinic Bi 2 O 3 -xRE 2 O 3 -yMoO 3 (RE=Pr, Nd, Sm, and Yb) based low temperature co-fired ceramics (LTCC) systems with high sintering density and low microwave dielectric loss are synthesized by conventional solid state reaction technique. The structure and dielectric properties of Bi 2 O 3 -xRE 2 O 3 -yMoO 3 ceramics are investigated. Dense BiNdMoO 6 ceramics sintered at 900 °C for 8 h in air have a low dielectric constant ε r =~7.5, a high quality factor Q × f =~ 24, 800 GHz at 7.0 GHz, and τ f =~−16 ppm/̊C. Especially, good chemical compatibility of BiNdMoO 6 with Ag electrodes is represented as well. In contrast, BiSmMoO 6 ceramics sintered at 1000 °C for 8 h show enhanced Q×f =~43, 700 GHz at 7.8 GHz with ε r = ~8.5 and τ f = ~−27 ppm/°C. Bi 2 O 3 -xRE 2 O 3 -yMoO 3 (RE=Pr, Nd, Sm, and Yb) based ceramics could be considered as promising microwave ceramics for LTCC applications.
研究领域:
材料科学
·
电子工程
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