SENSORS
·
2019
·Vol. 19
·期 23
·5152
·
IF 3.031
Qingmin Wei,Jianjuan Lin,Fa Liu,Changchun Wen,Na Li,Guobao Huang,Zhihui Luo
A surface-enhanced Raman scattering (SERS) tag is proposed for high-sensitivity detection of gibberellin A3(GA3). Silver nanoparticles (AgNPs) were synthesized using citrate reduction. 4-Mercaptobenzoic acid (MBA) was used for the Raman-labeled molecules, which were coupled to the surface of the AgNPs using sulfydryls. MBA was coated with silica using the Stöber method to prevent leakage. GA3antibodies were attached via the active functional groups N-Hydroxysuccinimide (NHS) and N-Ethyl-N’-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) to construct a novel[email protected]2SERS tags. The captured SERS substrates were fabricated through Fe3O4nanoparticles and gold nanoparticles (AuNPs) using chemical methods. These nanoparticles were characterized using ultraviolet-visible spectroscopy (UV–Vis), dynamic light scattering, Raman spectroscopy, transmission electron microscope (TEM), and X-ray diffraction (XRD). This[email protected]2SERS tags has a strong SERS signal based on characterizations via Raman spectroscopy. Based on antigen-antibody reaction, the[email protected]3O4nanoparticles can capture the GA3and[email protected]2SERS tags. Due to the increasing number of captured nanoprobes, the SERS signal from MBA was greatly enhanced, which favored the sensitive detection of GA3. The linear equation for the SERS signal was y = −13635x + 202211 (R2= 0.9867), and the limit of detection (LOD) was 10−10M. The proposed SERS tags are also applicable for the detection of other food risk factors.Keywords:surface-enhanced Raman scattering;[email protected]2core-shell nanoparticles;[email protected]3O4nanoparticle;gibberellin
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 144
·期 15
·4526-4533
·
IF 4.019
Pan Li,Meihong Ge,Chentai Cao,Dongyue Lin,Liangbao Yang
Development of analytical methods allowing sensitive detection of neurotransmitters in various biofluids is vital. However, limitations of these methods include interference of impurities and stringent requirements concerning sample purity. In the current work, we developed a strategy for the rapid and sensitive analysis of dopamine (DA) in various biofluids with a smart surface-enhanced Raman spectroscopy (SERS) probe composed of magnetite Fe3O4 and Au nanoparticles (Fe3O4/Au NPs). Besides the simple and quick separation of DA from the specimen, Fe3O4 not only enabled a specific chemical interaction with DA molecules, but also acted as a SERS substrate capable of electromagnetically enhancing the Raman signal of DA. Therefore, the Fe3O4/Au NP composite with its coexisting electric-field effect and charger transfer (CT) enhancement was found to be beneficial for capturing the target molecules in biological environments and then enhancing the DA sensitivity. To understand the strong binding interaction between Fe3O4/Au and DA, X-ray photoelectron spectroscopy (XPS) was carried out, specifically to illuminate the chemical adsorption or possible CT complex. Moreover, a rapid purification strategy for further separating DA from serum was developed, and thus a high nanometer-level sensitivity was achieved. In addition, the feasibility of using Fe3O4/Au combined with the developed purification method was also verified using various tissue homogenates spiked with DA molecules. Such a nanocomposite can offer the possibility of efficiently separating DA from the complex specimen and then providing the sensitive detection of DA for various tissues. Accordingly, the smart SERS Fe3O4/Au nanocomposite probe, with its advantages of simple pre-treatment and synergetic enhanced mechanisms, shows great promise for the rapid and sensitive detection of DA in complicated specimens.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Zhenjiang Li,Jifang Yin,Chenghai Gao,Guanhao Qiu,Alan Meng,Qingdang Li
Prostate specific antigen (PSA), as the significant biomarker of prostate cancer, has aroused widespread concern. However, constructing the PSA aptasensor with high sensitivity and low detection limit is still facing huge challenges. In this study, a sensitive electrochemical aptasensor for detecting PSA based on coral-like poly-aniline (PANI)/gold nano-particles (AuNPs) and the peptides was constructed. Coral-like PANI and AuNPs with the diameter of 50–80 nm, as the substrate materials, were directly deposited on glass carbon electrode (GCE) by electrodeposited method. Then, the peptides, as the antifouling materials, were anchored on the modified-electrode, which not only can form antifouling surface to reduce nonspecific adsorption, but also can be as a medium between the modified-electrode and PSA aptamer. Finally, the PSA aptamer was immobilized on the electrode. Based on the synergistic effect of excellent conductivity and special morphology of the substrate materials, and the antifouling ability of peptides, the aptasensor exhibited a high sensitivity of 462.7 μA(ng mL −1 ) −1 cm −2 , wide linear range from 0.1 pg mL -1 to 100 ng mL -1 , low limit of detection (LOD) of 0.085 pg mL -1 and good antifouling performance. The aptasensor also possessed outstanding selectivity, reproducibility and stability. Moreover, the aptasensor can directly detect PSA in 10% human serum solutions.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2019
·
IF 4.175
Yuanshuai Li,Haijun Lu,Yuting Wang,Xiaoli Li
A highly active and recyclable catalyst for the deposition of Au nanoparticles on the surface of polydopamine-functionalized poly(vinyl alcohol) macroporous beads was fabricated through the oxidative polymerization of dopamine and in-situ reduction route. The morphology and structure of PVA-PDA@Au beads were characterized using scanning electron microscopy, transmission electron microscopy , X-ray photoelectron spectroscopy, energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy , X-ray power diffraction and N 2 adsorption–desorption measurement. The as-prepared PVA-PDA@Au beads combined the advantages of PVA-PDA beads and Au NPs. PVA-PDA@Au beads exhibited enhanced catalytic activity towards the degradation of Rhodamine B (RhB) in the presence of NaBH 4 with removal efficiency up to 99.5% in 5 min at an initial RhB concentration of 100 mg/L and a catalyst amount of 0.5 g/L. PVA-PDA@Au beads could be easily separated and recycled from the reaction system by simple filtration, and reused for the degradation of RhB in six consecutive cycles with a conversion of >99%. Moreover, the mechanism for the degradation of RhB by PVA-PDA@Au beads was proposed. In addition, the catalytic ability of PVA-PDA@Au beads was further studied to remove Tetrabromobisphenol A (TBBPA), in which approximately 100% TBBPA (initial concentration of 25 mg/L) was removed within 90 min. In practical application, RhB as a pollutant in river water was degraded rapidly by PVA-PDA@Au beads with removal efficiency higher than 94%. Thus, PVA-PDA@Au beads possessed high catalytic activity, fast reduction rate and good reusability as well as easy separation and simple preparation, endowing this catalyst with potential application prospect in the treatment of wastewater containing organic pollutants.
✓ 已验证
产品关联
DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2019
·
IF 14.229
Ting Bian,Beibei Xiao,Biao Sun,Long Huang,Shan Su,Yi Jiang,Jiankun Xiao,Aihua Yuan,Hui Zhang,Deren Yang
The ultimate goal of catalyst design is to optimize catalytic performance by revealing the relationship between structure and activity. However, it remains tremendously challenging to distinguish the role of electronic and ensemble effects in bimetallic core-shell electrocatalysts for hydrogen evolution reaction (HER). For this purpose, we demonstrate an efficient approach to local epitaxial growth of Rh atoms on Au decahedra to investigate the above-mentioned two effects. Among them, Au 75 Rh 25 core-shell star-shaped decahedra exhibited the best activity for HER with an overpotential of 64.1 mV at a current density of 10 mA cm −2 , which is about 39.4 mV less than that of commercial Rh/C. Density function theory (DFT) calculations revealed that ensemble effect is the dominant factor to accelerate the reaction kinetics of HER by exposing Au atoms on the surface and thus weakening the strong adsorption of the intermediates on Rh surface.
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Shuli Lv,Jinliang Sheng,Shiyi Zhao,Mingchao Liu,Lihua Chen
It is a novel competitive challenge for electrochemical biosensor to directly, rapidly and ultrasensitively detect the disease markers in the whole serum due to biofouling caused by the complexity of actual samples. In this paper, poly (ethylene glycol) (PEG) and hyaluronic acid (HA) were utilized to modify Fe 3 O 4 @Au nanoparticles (NPs). Based on the successfully preparation and characterization of Fe 3 O 4 @ [email protected] @HA NPs with TEM , SEM, XRD, FTIR and EDS , respectively, a novel immunosensor of brucellosis with high selectivity , sensitivity and almost perfect protein-resistant properties in various external environments, especially, in complex biological systems was fabricated. More importantly, this immunosensor is capable of assaying brucellosis antibody in 100% serum without suffering from any significant biological interference. In addition, a wide linear response range from 10 –15 g mL −1 to 10 –11 g mL −1 towards antibody in 100% serum and a low limit of detection (LOD) of 0.36 fg mL −1 (3σ, n = 13) are demonstrated, which indicates that this immunosensor has a promising potential in clinical diagnosis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2018
·
IF 5.116
Peipei Qi,Jiao Wang,Xue Wang,Xiangyun Wang,Zhiwei Wang,Hao Xu,Shanshan Di,Qiang Wang,Xinquan Wang
An efficient, highly sensitive and selective electrochemical sensor was developed for determination of fenitrothion in tap water, cropland water and canal water samples. The synthesized reduced graphene oxide (RGO) and molybdenum sulfide (MoS 2 )-Au were introduced using their synergetic enhancement effect to improve the sensor sensitivity. Zirconia (ZrO 2 ) nanoparticles were electro-deposited onto modified gold electrode to selectively adsorb fenitrothion, which can be further captured by cyclic voltammetry or square wave voltammetry on the basis of its redox behavior of nitrobenzene group. The immobilization process was characterized by electrochemical impedance spectroscopy , illustrating the enhancement of RGO/MoS 2 -Au composite and the successful electro-deposition of ZrO 2 . Systematical method validation was performed including linearity, sensitivity, selectivity, reproducibility, regeneration and stability. The current is linearly correlated with the fenitrothion concentration from 5.0 to 600 ng mL −1 with the limit of detection of 2.2 ng mL −1 . The developed electrochemical sensor displayed satisfactory performance in real water samples analysis.
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Lei Wang,Tianjiao Meng,Guangshun Yu,Shasha Wu,Jingjing Sun,Huixian Jia,Huan Wang,Xinjian Yang,Yufan Zhang
An electrochemical biosensor was designed for the determination of telomerase activity using an enzyme-free, PCR-free, and convenient electrochemical strategy. In this work, the electrochemical biosensor was constructed through the functionalization of Au nanorods with a carboxylic group (AuNRs-3) and subsequent immobilization with capture DNA (cDNA) for sensing telomerase activity. Upon telomerase triggered extension, the telomerase activity is related to the amount of the adsorbed electrocatalyst , leading to the different electrochemical signals for readout. Integrating with the efficient electrocatalysis of AuNRs-3-cDNA towards oxidation of acetaminophen, the prepared biosensor exhibits a wide dynamic correlation of telomerase activity from 1 × 10 2 to 1.04 × 10 7 HeLa cells mL −1 with a sensitivity of 2.68 HeLa cell mL −1 and the limit of detection was calculated to be 52.81 HeLa cells mL −1 under the optimal experimental conditions. Furthermore, the application of this electrochemical biosensor would provide the great potential for analysis of telomerase activity, revealing a powerful platform for early diagnosis of cancers.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 47
·7862-7870
·
IF 4.776
Jian Chen,Dongya Zhang,Yan Zou,Zhongjie Wang,Mingcong Hao,Meng Zheng,Xue Xue,Xiaoxi Pan,Yiqing Lu,Jiefei Wang,Bingyang Shi
Nanohybrids fabricated with upconversion nanoparticles (UCNPs) and gold nanoparticles (NPs) hold great promise for near-infrared photothermal therapy (NIR-PTT) and upconversion fluorescence imaging. However, the obstacles of poor solubility in water and finite hydrophilic modification method for UCNPs limit their application in biological fields. Herein, we report a novel UCNP@Al(OH)3/Au nanohybrid mediated by a highly hydrophilic and biocompatible Al(OH)3 layer to realize a synergistic targeted PTT and fluorescence imaging capability to U87MG tumor-bearing mice under NIR light irradiation. The modification with Al(OH)3 layers can improve the water solubility of UCNPs. And cytotoxicity assays and hemolysis assay showed that the modification with Al(OH)3 layers makes UCNPs have low cytotoxicity and good biocompatibility. In addition, the Al(OH)3 layers are thin enough to allow fluorescence resonance energy transfer (FRET) between UCNPs and gold NPs to occur, giving the NPs a good PTT effect for tumor-bearing mice. Meanwhile, as the pH-sensitive Al(OH)3 layers decompose in acidic tumor microenvironments with Au NPs detached from their surface, the FRET effect no longer occurred, subsequently leading to the fluorescence intensity of naked UCNPs being recovered for good imaging effects. The study suggests that Al(OH)3 mediation layer as a promising hydrophilic nanoplatform can potentially be used for the preparation of superior hydrophilic NPs and pH-stimulated drug release carriers for theranostic application in vivo.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2013
·
IF 5.437
Shenguang Ge,Weiyan Liu,Lei Ge,Mei Yan,Jixian Yan,Jiadong Huang,Jinghua Yu
A simple, low-cost and sensitive origami electrochemical immunoassay-device was developed based on a novel gold nanoparticle modified porous paper working electrode (Au-PWE) for point-of-care testing. Azide-functionalized Au-PWE was prepared by the functionalization of Au-PWE with 1-azidoundecan-11-thiol. Alkyne end-terminated antibody was prepared with 4-pentynoic acid and antibody by the 1-ethyl-3-(3-(dimethylamino) propyl) carbodiimide hydrochloride and N-hydroxysuccinimide activation reaction . Alkyne-antibody was coupled to azido-Au-PWE by click reaction as a recognition element. Nearly monodispersed sphere-like silica-coated ferroferric oxide (Fe 3 O 4 @SiO 2 ) nanoparticles were prepared via the reverse microemulsion method. Azide-functionalized Fe 3 O 4 @SiO 2 was prepared by the functionalization of silica shell with 3-bromopropyltrichlorosilane followed by substitution with sodium azide . Alkyne-functionalized antibody and horse radish peroxidase were coupled to azide-functionalized Fe 3 O 4 @SiO 2 by click reaction as signal label. Horse radish peroxidase and ferroferric oxide could catalyze the oxidation of thionine in the presence of hydrogen peroxide. After the sandwich immunoreaction, the current was proportional to the logarithm of the Microcystin-LR. The linear regression equation was i (μA)=119.89+46.27 log c MC-LR (μg/mL) in the range from 0.01 to 200 μg/mL. The limit of detection was 0.004 μg/mL. This immunoassay would provide a universal immunoassay method in environmental monitoring and public health.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2015
·
IF 3.545
Junrong Li,Guannan Zhang,Lihua Wang,Aiguo Shen,Jiming Hu
In this work, we suggest a chitosan-modified popcorn-like Au–Ag nanoparticles (CSPNPs) based assay for high sensitive detection of melamine , in which CSPNPs not only provide with an intrinsic peroxidase-like activity but also act as surface enhanced Raman scattering (SERS) substrates. CSPNPs can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 to the charge transfer complex (CTC), which contributes to a tremendous surface-enhanced resonant Raman scattering (SERRS) signals with 632.8 nm laser excitation. The target molecule melamine can generate an additional compound with H 2 O 2 , which means the available amount of H 2 O 2 for the oxidation of TMB reduced. Correspondingly, the SERRS intensity of CTC is decreased. The decreased Raman intensity is proportional to the concentration of melamine over a wide range from 10 nM to 50 μM ( R 2 =0.989), with a limit of detection (LOD) of 8.51 nM. Moreover, the proposed highly selective method is fully capable of rapid, separation-free detection of melamine in milk powder.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 30
·4363-4370
·
IF 1.9
Chi-Fang Peng,Ying-Ying Zhang,Li-Ying Wang,Zheng-Yu Jin,Guang Shao
In this study, peroxidase-mimicking core–shell Au@Pt nanoparticles (Au@Pt NPs) were synthesized, and both Hg2+ and Ag+ were found to intensively inhibit the catalytic activity of the Au@Pt NPs. The interactions between the two metal ions and Au@Pt NPs were analyzed through characterization by TEM, DLS, EPR and XPS, etc. Based on the inhibition of both Hg2+ and Ag+ toward the catalytic activity of the Au@Pt NPs and the shielding of sodium dodecyl sulfate (SDS) toward interfering metal ions, a highly sensitive and selective colorimetric assay for Hg2+ and Ag+ was developed. In addition, L-cysteine can be used to mask Hg2+ in the presence of Ag+; therefore, a specific detection of Ag+ ions can be accomplished by this Au@Pt NP-based assay. The limits of detection (LODs) for Hg2+ and Ag+ are 3.5 nM and 2.0 nM, respectively. The linear ranges for detecting Hg2+ and Ag+ are from 10 nM to 200 nM and from 5.0 nM to 100 nM, respectively. Moreover, the colorimetric assay is highly selective toward Hg2+ and Ag+ over other common metal ions. This work demonstrates that the optimization of metal nanoparticle preparation and surface modification play very important roles in developing metal ion sensing based on metal NP enzyme mimics. In addition, the metallic nanozyme based colorimetric method here demonstrates advantages in practical applications as it is low-cost, simple, and highly sensitive and selective, although it needs a two-step reaction.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）