Frontiers in Bioengineering and Biotechnology
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2021
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IF 5.89
Wu Xiaodong,Li Pengpeng,Cheng Jian,Xu Qiang,Lu Beiji,Han Conghui,Huo Weiling
Objective: Osteoarthritis (OA) is a common subtype of arthritis. To date, treatment of OA focuses primarily on alleviating pain and improving joint function. The lack of a vascular system within synovial joints and the rapid removal of agents due to synovial exchange hinder continuous delivery of OA drugs. However, these obstacles are being addressed by promising nanoscale drugs.Methods: We synthesize and assemble a hydrogen peroxide [H2O2, belongs to the category of active oxygen species (ROS)]-sensitive nanomicelle, which is loaded with the anti-inflammation drug dexamethasone and chondrogenic differentiation factor cartilage-derivedmor-phogeneticprotein-1. The micelle can induce bone marrow mesenchymal stem cells to repair cartilage while inhibiting joint inflammation.Results: The prepared nanoparticles were of uniform size and displayed an obvious core-shell structure. Under H2O2 stimulation, the shell layer could be removed gradually. The drug-loaded micelle effectively inhibited proliferation of activated macrophages, induced macrophage apoptosis with an anti-inflammatory effect, and caused the BMSCs to differentiate into chondrocytes.Conclusion: This work provides an experimental and theoretical basis for further development of a drug-loaded micelle in the healing of osteoarthritis.
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RSC Advances
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2021
·Vol. 11
·期 11
·5874-5884
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IF 3.361
Sheng Tang,Xinyu Xiao,Jing Hu,Bo Gao,Hunglin Chen,Zhuang Zuo,Qi Qi,Zongyang Peng,Jianchun Wen,Dechun Zou
Organic–inorganic hybrid perovskites have emerged as promising light harvesting materials for many optoelectronic devices. Here, we present a facile mechanochemical synthesis (MCS) route for the preparation of a series of pure phase mixed-cation/anion (FAPbI3)x(MAPbBr3)1−x (0 ≤ x ≤ 1) hybrid perovskite materials for high-efficiency thin-film perovskite solar cells (PSCs). The use of (α-FAPbI3)0.95(MAPbBr3)0.05 perovskite prepared by MCS for the thin-film PSCs achieves a maximum PCE of 15.9% from a current–voltage (J–V) scan, which stabilises at 15.4% after 120 s of the maximum power point output. Furthermore, PSCs based on (KPbI3)0.05(FAPbI3)0.9(MAPbBr3)0.05 perovskite prepared by MCS exhibit higher photovoltaic performance and lower hysteresis compared with (α-FAPbI3)0.95(MAPbBr3)0.05, with a maximum PCE of 16.7%. These results indicate that the use of mechanochemically synthesised perovskites provides a promising strategy for high performance PSCs and superior control in optoelectronic properties, leading to improved control in fabrication approaches and facilitating the development of efficient and stable PSCs in the future.
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JOURNAL OF CHROMATOGRAPHY A
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2020
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IF 4.049
Jiaxuan Fan,Zikai Liu,Jing Li,Wenfeng Zhou,Haixiang Gao,Sanbing Zhang,Runhua Lu
It is necessary to establish a rapid, simple and environmentally friendly detection method for benzoylurea pesticides (BUs) in environmental water samples because of their toxicity in environmental circulation. Herein, a novel polyethylene glycol (PEG) modified magnetic covalent organic framework material based on the Schiff base reaction was prepared (PEG/Fe 3 O 4 @SNW-1). This material was used as a sorbent for enriching five BUs by magnetic solid phase extraction prior to detection via high-performance liquid chromatography. After the optimization of several parameters (such as the salinity, extraction time, amount of sorbent, desorption time, etc.) that influenced the recovery of the magnetic solid phase extraction process, the limits of detection (S/N = 3) were defined as less than 1.0 μg L −1 , and the limits of quantitation (S/N = 10) were calculated as being lower than 3.4 μg L −1 . A satisfactory linear range of 5–1000 μg L −1 was achieved. Finally, the proposed method was applied to analyze benzoylurea pesticides in three environmental water samples. These results indicated that the proposed method was feasible and demonstrated the potential application of the PEG/Fe 3 O 4 @SNW-1 material for detecting similar pesticide residues in environmental water samples.
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Science Advances
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2020
·Vol. 6
·期 6
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IF 13.117
Zecong Xiao,Zhenwei Su,Shisong Han,Jinsheng Huang,Liteng Lin,Xintao Shuai
The response to programmed cell death protein-1 (PD-1)/programmed death ligand-1 (PD-L1) blockade in cancer immunotherapy is limited because of multiple immune evasion mechanisms. Here, a previously unknown strategy is proposed to synergize the nuclear factor κB (NF-κB) inhibition and PD-1 blockade for antitumor immunotherapy. A dual pH-sensitive nanocarrier loading curcumin (CUR) and anti–PD-1 monoclonal antibody (aPD-1) may bind to circulating PD-1+ T cells and then follow their infiltration into the tumor. Furthermore, the nanodrug bound to PD-1+ T cells may be released in the tumor microenvironment, leaving aPD-1 to block PD-1 on T cells and generating a CUR-encapsulated cationic nanodrug that can be easily taken up by tumor cells/tumor associated macrophages (TAMs). Thus, not only the antitumor T cells mediate efficient CUR delivery to tumor but also the efficient CUR delivery promotes the tumor infiltration of antitumor T cells, thereby resulting in effective activation of antitumor immunity.
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Frontiers in Pharmacology
·
2020
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IF 4.225
Yao He,Sun Jingyu,Wei Jie,Zhang Xin,Chen Bing,Lin Yajun
Over recent years, an increasing number of studies have confirmed that the occurrence and development of vascular pathological changes are closely related to oxidative stress and the inflammatory response of the vascular endothelium. Kaempferol is the most common flavonoid compound found in fruits and vegetables. Our present research identified that kaempferol had the capability to protect the vascular endothelium in a mouse model of vascular injury and explored the specific mechanisms underlying these effects by investigating oxidative stress, the extent of cardiovascular injury, and inflammatory markers such as NF-κB, TNF-α, IL-6, and the Nrf2/HO-1 signaling pathway. Analysis showed that kaempferol reduced oxidative stress and inflammation mediated by H2O2 and paraquat, respectively, both in vitro and in vivo. Furthermore, kaempferol suppressed the levels of TNF-α and IL-6, and the activation of NF-κB, in aortic tissues and human umbilical vein endothelial cells (HUVECs). Finally, we observed that kaempferol corrected the levels of antioxidants and elevated the protein levels of Nrf2 and HO-1 in aortic tissues and HUVECs. Collectively, our findings prove that kaempferol protects blood vessels from damage induced by oxidative stress and inflammation and that the Nrf2/HO-1 signaling pathway plays a key role in mediating these effects.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2020
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IF 3.573
Zheng Zhang,Gui-Ping Cao,Qian Cai,Hui Lu,Shuang Ji,Rui Fang,Peng Gao,Miao Feng
The TS-1 particles grown on carbon nanotubes decorated nickel foam (denoted as MTS-1S) are successfully synthesized by a steam-assisted crystallization method that does not waste the gels and is more convenient, economical, and efficient than the traditional hydrothermal method. MTS-1S possesses a hierarchical porous structure of micropores, mesopores, and macropores and a high content of titanium in the skeleton, which is beneficial for the diffusion of reactants and products and exhibits excellent catalytic performance for the epoxidation of propylene. The impact of water on the formation of acid sites has been demonstrated by density functional theory (DFT). The acid sites of MTS-1S can be tuned by controlling the amount of water at the bottom of autoclave during steam-assisted crystallization, which significantly increases the selectivity to propylene oxide. Finally, the impact of acid sites of Si–OH and Ti–OH groups (Ti located in a defect structure, adjacent to Si–OH) on the byreaction of epoxidation of propylene is confirmed by simulation.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2020
·Vol. 27
·期 11
·11990-12000
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IF 3.056
Cui Yanping,Thathsarani Nimesha,Peng Liang,Gao Ya,Lei Lidan,Zhou Zhengwei,Liang Lili,Shi Xiaoyan
Innovative paramagnetic one-dimensional (1-D) core-shell Fe-Ag@AgCl visible light-driven photocatalysts are synthesized through a template-assisted electrodeposition method trailed by FeCl 3 in-situ oxidation. The metallic nature of Fe-Ag@AgCl is confirmed through scanning electron microscopy (SEM) and crystal nature through X-ray diffraction (XRD). The controllable diameter of Fe-Ag is obtained through the selection of hollow size of the polycarbonate (PC) template. Electron impedance spectroscopy (EIS) confirms through the introduction of Fe to the Ag core that has prolonged the recombination of electron and hole. Escherichia coli ( E. coli ) are employed as the target bacteria to evaluate the photocatalytic disinfection performances. A total of 1.30 mg of Fe-Ag@AgCl is proved to be able to completely inactivate 10 7 CFU (colony forming units)/mL after 120 min of visible light irradiation. The transition electron microscopy (TEM) confirms the stability of the material after the photo reaction. As Fe-Ag@AgCl possesses magnetic properties, the material is recovered through the application of an external magnetic field. SEM images and results of 3D emission extraction matrix (EEM) depict that the bacteria cell death is caused by membrane permeability changes caused by the reduction of membrane associated proteins.
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LIFE SCIENCES
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2020
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IF 3.647
Tian Li,Li Tu,Ran Gu,Xiu-Lin Yang,Xiu-Juan Liu,Gui-Ping Zhang,Qian Wang,Yi-Pin Ren,Bing-Jin Wang,Jin-Yong Tian
Objectives To investigate the effect of glucagon-like peptide-1 (GLP-1) receptor and glucose dependent insulinotrophic polypeptide (GIP) receptor dual agonist DA-JC4 on alleviating Parkinson's disease (PD) and unveil related cellular mechanisms. Methods Rotenone was injected to generate a rat PD model, on which the effect of DA-JC4 on motor functions was evaluated by rotational behavioral assay and open field test. The survival of dopaminergic neurons was analyzed, in addition to assays for mitochondrial stress and quantification of neurotransmitter levels using high performance liquid chromatography (HPLC). In cultured hippocampal neurons, the effect of DA-JC4 on mitochondrial stress and related cellular mechanism was analyzed by Flow cytometry, western blotting and reactive oxygen species (ROS). Results DA-JC4 significantly improved motor functions in PD rats, and elevated levels of major neurotransmitters. By histological analysis, DA-JC4 protected dopaminergic neurons from rotenone-induced cell death, which was associated with reduced mitochondrial stress. Experiments in cultured rat hippocampal neurons validated the neuroprotective role of DA-JC4 against cell apoptosis and mitochondrial stress induced by rotenone. The protective effect of DA-JC4 was later found to be dependent on AKT/JNK signal pathway, as treatment using AKT inhibitor or JNK activator abolished such effects. Conclusion Our results showed that the dual agonist of GLP-1/GIP receptor could ameliorate motor dysfunctions of PD by protecting dopaminergic neurons which was mediated by relieved mitochondrial stress and apoptosis via AKT/JNK signal pathway.
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Reaction Chemistry & Engineering
·
2020
·Vol. 5
·期 8
·1397-1404
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IF 3.441
Xin Pan,Sihua Wu,Deshan Yao,Lian Liu,Lina Zhang,Zixuan Yao,Yan Pan,Siyuan Chang,Bingfeng Li
5-Hydroxymethyl-2-furancarboxylic acid (HMFCA) is a significant added-value intermediate derivatized from the bio-based platform chemical 5-hydroxymethylfurfuraldehyde (HMF). In the HMFCA biosynthesis process, the catalytic performance of the biocatalyst always decreases due to the presence of HMF toxicity, which is not conducive to achieving high product accumulation. Herein, we successfully obtained a new whole-cell biocatalyst from Pseudomonas aeruginosa PC-1, which was first reported to produce HMFCA from HMF. After optimization, 90.1% yield of HMFCA was attained within 6 h using a 100 mM substrate. Importantly, a much lower cell dosage was needed in the process. In order to achieve higher HMFCA accumulation, a fed-batch strategy was developed by using the growing cells from P. aeruginosa PC-1. Consequently, a total concentration of 721 mM HMFCA was obtained from 800 mM HMF within 58 h after 8 cycles of fed-batch, which is the highest concentration to date. The efficient HMFCA production made P. aeruginosa PC-1 a promising whole-cell biocatalyst for selective, large scale biotransformation of HMF.
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JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
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2020
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IF 5.278
Lei Cai,Chao Chen,Wenjun Wang,Xiu Gao,Xiaoyan Kuang,Yan Jiang,Liang Li,Guoqiang Wu
Titanium silicalite-1 (TS-1) zeolite was successfully synthesized by hydrothermal method in the presence of the tetrapropylammonium hydroxide (TPAOH) template, and a series of TS-1 supported cadmium with different Cd loading ( x Cd/TS-1) catalysts were carefully prepared to produce epoxidized soybean oil (ESO) through the acid-free catalytic epoxidation of soybean oil (SO) with H 2 O 2 . These catalysts were fully characterized using various modern techniques and further evaluated systematically in this epoxidation reaction to understand the relationship between catalytic activity and structure of active components. The results show that the highly dispersed Cd species are introduced on the TS-1 support without affecting the coordination environment of tetrahedral Ti (framework Ti). Furthermore, the Cd species are coordinated with tetrahedral Ti species via O atom resulting in the weakness of the Ti–O bonds and facile attack of the Ti–O bonds by alcohol and H 2 O 2 , which can easily form the five-membered intermediate and promote the SO epoxidation process. Furthermore, the deactivation and regeneration behaviors of 2%Cd/TS-1 catalyst in SO epoxidation were also investigated, which reveal that the catalyst deactivation of coke deposition is due to the strong adsorption of the catalysts and SO molecules. But the spent 2%Cd/TS-1 can be completely regenerated by calcination method.
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JOURNAL OF MOLECULAR LIQUIDS
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2020
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IF 5.065
Xiaojie Liu,Chunying Zhu,Taotao Fu,Youguang Ma
The densities and viscosities of ternary solution of piperazine (PZ) + 2-Amino-2-methyl-1-propanol (AMP) + H 2 O were measured at P = 101 kPa and T = (303.15–343.15) K. The effects of temperature and concentration on the density and viscosity of ternary solution were investigated. The apparent molar volume ( V φ ), the limiting partial molar volume ( V φ 0 ), viscosity B -coefficient, Helper's constant and the free energies of activation per mole of solvent ( Δμ 1 0≠ ) and solute ( Δμ 2 0≠ ) were calculated from the density and viscosity, respectively. The solute-solute and solvent-solute interactions were analyzed from the volumetric and viscometric properties. The results show that the PZ has a destructive effect on the structure of mixed solvent.
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WATER RESEARCH
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2019
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IF 7.913
Meng Jiang,Xiong Zheng,Yinguang Chen
Bio-denitrification (i.e., microbial reduction of nitrate to gaseous nitrogen) is usually reported to be affected by operating and environmental parameters, such as carbon source type, pH value, and temperature. In this paper, the enhancement of denitrification performance with the elimination of nitrite accumulation and nitrous oxide emission by Shewanella oneidensis MR-1 were investigated and the mechanisms were explored. It was found that S. oneidensis MR-1 itself had marginal nitrate removal capacity, but its presence in the denitrification system of a well-studied denitrifier (Paracoccus denitrificans ) obviously enhanced nitrate removal efficiency (from 65.3% to 97.8%) and reduced nitrite accumulation (0.67 against none-detectable) and N 2 O generation (from 8.87 μm/mM-TN to none-detectable). The mechanism study showed that S. oneidensis MR-1 promoted electrons transfer activity via the formation of nanotube between cells, which resulted in the increase of denitrification enzymes activity , carbon source metabolism, ATP level and cell viability. As the generation, transfer and consumption of electrons were enhanced by S. oneidensis MR-1, the improvement of denitrification performance with reduction of nitrite accumulation and N 2 O emission was therefore achieved. Finally, the performance of denitrification enhanced by S. oneidensis MR-1 was testified by laboratory groundwater treatment experiment. This study suggested the potential role of S. oneidensis MR-1 in accelerating nitrate bio-transformation in nitrogen geochemical cycle and increasing bio-treatment of nitrate contamination with negligible harmful intermediates (nitrite and N 2 O) accumulation.
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