ACS Applied Materials & Interfaces
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2017
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IF 7.504
Yunsu Ma,Yao Cen,Muhammad Sohail,Guanhong Xu,Fangdi Wei,Menglan Shi,Xiaoman Xu,Yueyue Song,Yujie Ma,Qin Hu
In this work, a new kind of N, Cu codoped carbon dots (N/Cu-CDs) was prepared via a facile one-pot hydrothermal method by using citric acid monohydrate, copper acetate monohydrate and diethylenetriamine. The prepared N/Cu-CDs with a high quantum yield (50.1%) showed excitation-independent emission at 460 nm. The structure and fluorescence properties of N/Cu-CDs were characterized by high-resolution transmission electron microscopy, fluorescence spectrofluorometer, FT-IR spectrometer, UV–visible spectrophotometer and X-ray photoelectron spectroscopy. N/Cu-CDs were applied to establishing a ratiometric fluorescence probe toward H2O2 based on the inner filter effect (IFE) between N/Cu-CDs and DAP (2,3-diaminophenazine, the oxidative product of o-phenylenediamine (OPD)), and provided a ratiometric fluorescence universal platform for detection of the metabolites participating in H2O2-generation reactions (cholesterol and xanthine). The proposed method was demonstrated to be ultrasensitive and highly selective for cholesterol and xanthine assay with detection limits of 0.03 and 0.10 μM, respectively. The fluorescence probe built was applied to the determination of cholesterol and xanthine in human serum with satisfactory results.
研究领域:
分析化学
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生物化学
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材料科学
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DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
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2024
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IF 4.2
Minghui Cai,Qiuyuan Li,Yang Cao,Yujia Huang,Haixu Yao,Chen Zhao,Jiao Wang,Hui Zhu
Autophagy is closely related to the aging of various organ systems, including ovaries. Quercetin has a variety of biological activities , including potential regulation of autophagy. However, whether quercetin-regulated autophagy activity affects the process of ovarian aging and injury has not been clarified yet. This study explores whether quercetin can resist H 2 O 2 -induced aging and injury of granulosa cells by regulating autophagy and its related molecular mechanisms in vitro experiments. The cell viability , endocrine function, cell aging , and apoptosis were detected to evaluate the effects of quercetin and autophagy regulators like 3-methyladenine and rapamycin . The levels of autophagy markers Atg5, Atg12, Atg16L, Lc3B II/I, and Beclin1 were determined by Western blot to assess the effects of quercetin, 3-methyladenine and rapamycin on autophagy. Our results showed quercetin resisted H 2 O 2 -induced granulosa cell aging and injury by activating protective autophagy. The treatment of 3-methyladenine and rapamycin confirmed the protective function of autophagy in H 2 O 2 -induced granulosa cells. 3-methyladenine treatment inhibited the expression of autophagy markers Atg5, Atg12, Atg16L, Lc3B II/I, and Beclin1 and abolished the positive effects on cell viability , estradiol secretion, and cell apoptosis activated by quercetin. In conclusion, quercetin activates autophagy by upregulating the expression of autophagy-related proteins to resist H 2 O 2 -induced aging and injury, which is crucial for stabilizing the function of granulosa cells under oxidative injury conditions and delaying aging. This study may explain the protective effects of quercetin on ovarian aging and injury from the perspective of regulating autophagy.
研究领域:
生殖生物学
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药理学
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细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2023
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IF 7.4
Fangshuo Liu,Shiyu Xia,Ala’a Mhmoued Abdllh Alboull,Zhiyong Dong,Hongzhan Liu,Chengda Meng,Fengxia Wu,Guobao Xu
Metal-organic gels (MOGs) are a category of metal-organic smart soft materials with large specific surface areas, loose porous structures, and open metal active sites. In this work, trimetallic Fe(III)Co(II)Ni(II)-based MOGs (FeCoNi-MOGs) were synthesized at room temperature via a simple and mild one-step procedure. Fe3+, Co2+, and Ni2+ were the three central metal ions in it, while 1,3,5-benzenetricarboxylic acid (H3BTC) served as the ligand. The solvent enclosed in it was then removed by freeze-drying to get the corresponding metal-organic xerogels (MOXs). The as-prepared FeCoNi-MOXs have excellent peroxidase-like activity and can significantly enhance luminol/H2O2 chemiluminescence (CL) by more than 3000 times, which is very effective compared with other reported MOXs. Based on the inhibitory effect of dopamine on the CL of the FeCoNi-MOXs/luminol/H2O2 system, a simple, rapid, sensitive, and selective CL method for dopamine detection was established with a linear range of 5–1000 nM and a limit of detection of 2.9 nM (LOD, S/N = 3). Furthermore, it has been effectively used for the quantitative measurement of dopamine in dopamine injections and human serum samples, with a recovery rate of 99.5–109.1%. This research brings up prospects for the application of MOXs with peroxidase-like activity in CL.
研究领域:
材料科学
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分析化学
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生物传感器
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2020
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IF 6.306
Yao Pan,Ze-Yuan Deng,Xuan Chen,Bing Zhang,Yawei Fan,Hongyan Li
Phenolic acids (caffeic acid, p -coumaric acid,) and carotenes (β-carotene, lycopene) were mixed in different ratios to investigate antioxidant interactions on H 2 O 2 -induced H9c2 cells with ezetimibe (inhibitor of carotenes membrane transporters). Cellular uptake of carotenes, expression of membrane transporters, reactive oxygen species (ROS), nuclear factor erythroid 2-related factor 2 (Nrf2), NAD(P)H dehydrogenase quinone1 (NQO1), heme oxygenase-1 (HO-1), glutamate-cysteine ligase catalytic subunit (GCLC) were analyzed. Results revealed that phenolic acids increased cellular uptake of carotenes and expression of their membrane transporters. Combination groups contained more phenolic acids showed synergistic effects. For example, β-carotene: caffeic acid = 1:2 significantly suppressed the intracellular ROS (+EZT, 66.34 ± 51.53%) and enhanced the accumulation of nucleus-Nrf2 (+EZT, 30.23 ± 5.30) compared to the groups contained more β-carotene (+EZT, ROS: 75.48 ± 2.55%, nucleus-Nrf2: 19.48 ± 4.22). This study provided an implication of functional foods formulation and demonstrated that antioxidant synergism may due to the up-regulation of carotenes membrane transporters by phenolic acids.
研究领域:
药理学
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细胞生物学
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营养学
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PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
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2020
·Vol. 44
·期 41
·17984-17992
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IF 3.288
Guoning Chen,Qianqian Hu,Hua Shu,Lu Wang,Xia Cui,Jili Han,Kamran Bashir,Zhimin Luo,Chun Chang,Qiang Fu
The sensing method for hydrogen peroxide could also be employed to detect H2O2-bioprecursors in the presence of oxidase. However, the sensitivity of the conventional method is limited, and the catalytic stability and recyclability of enzymes also need to be improved. In this study, glucose was chosen as a typical representative H2O2-bioprecursor to design a fluorescence sensing system for H2O2-bioprecursors based on a magnetic immobilized enzyme and CdTe quantum dots. The magnetic immobilized enzyme was fabricated by a cross-linking method to enhance the stability and recyclability of the enzyme. The obtained product was characterized by scanning electron microscopy, transmission electron microscopy, energy dispersive spectrometry, and vibrating sample magnetometry, respectively. The CdTe quantum dots were prepared by a hydrothermal method, and revealed a maximum emission peak at about 610 nm under the maximum excitation light (339 nm). The developed CdTe QDs exhibited excellent sensing performance for hydrogen peroxide. The prepared immobilized enzyme and quantum dots were used to develop a fluorescence sensing system and were successfully exploited to detect a typical H2O2-bioprecursor (glucose) in the presence of glucose oxidase. The method exhibited good linear performance in the concentration range of 1–20 μg mL−1, and good recoveries ranging from 106.2–117.7%. This study demonstrated that the strategy could be extended to detect more H2O2-bioprecursors.
研究领域:
分析化学
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生物化学
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纳米技术
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生物医学工程
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DOI
高影响因子
Inorganic Chemistry Frontiers
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2022
·Vol. 9
·期 11
·2544-2556
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IF 7.779
Xuwen Da,Zhanhua Wang,Yao Jian,Chao Zhang,Yuanjun Hou,Yishan Yao,Xuesong Wang,Qianxiong Zhou
The bottleneck problems of chemodynamic therapy (CDT) are the dependence on an acidic pH (2.0–4.0), insufficient H2O2 level in tumor tissues, and potential systematic toxicity of inorganic nanomaterials-based CDT agents. In this study, the new tricks of two old compounds, i.e. [Ru(phen)2(PIP-OCH3)](PF6)2 (Ru3, phen = 1,10-phenanthroline, PIP-OCH3 = 2-(4-methoxy phenyl)-1H-imidazo[4,5-f][1,10]phenanthroline) and Fe(tpy)Cl3 (tpy = 2,2′:6′,2′-terpyridine), were harnessed to construct an ideal CDT system. Ru3 was able to efficiently photo-catalyze NADH oxidation accompanied by the production of H2O2in situ with a high yield of 73.2%, which can be converted into cytotoxic hydroxyl radicals by the biocompatible Fe(tpy)Cl3 at a neutral pH. By loading both Ru3 and Fe(tpy)Cl3 in tumor-targeting bovine serum albumin (BSA), the obtained BSA@Fe/Ru nanoparticles exhibited a highly efficient, selective, and photo-controlled CDT activity both in vitro and in vivo. This work may provide new sights for overcoming the issues confronted in CDT.
研究领域:
纳米技术
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癌症治疗
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光催化
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生物医学工程
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
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IF 7.46
Yang Li,Jia-Jia Li,Qing Zhang,Jian-Yong Zhang,Na Zhang,Yong-Zheng Fang,Jinwu Yan,Qinfei Ke
The highly emissive BODIPY (boron-dipyrromethene) have been grafted on the Eu-based metal-organic frameworks (Eu-MOFs) via the covalent F-B bonding using a facile one-pot solvothermal reaction. The fluorescence emission ratio of [email protected] has been applied to construct the ratiometric fluorescence sensor for the F - , H 2 O 2 and glucose detection in water solution and living cells. The produced [email protected] probe provided enhanced sensitivity, higher selectivity and lower lower limits of detection (LOD) for the detection of F - (0.1737 μM), H 2 O 2 (6.22 nM) and glucose (6.92 nM), and 5.96, 6.52 and 6.95 times lower than that for single Eu-MOF, respectively. More importantly, the probe also exhibited the lower biotoxic in living cells for bioimaging and the better visualization performance with multiplex channels. The results indicated the π-conjugated BODIPY derivative not only acted as the surfactant molecule or template agent for preferred growth, but also modulated the fluorescent emissions drastically, and thus induced excellent fluorescent detecting performances.
研究领域:
纳米技术
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分析化学
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生物医学工程
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生物化学
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DOI
高影响因子
International Journal of Environmental Research and Public Health
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2022
·Vol. 19
·期 23
·15688
·
IF 4.614
Yifan Yao,Kai Zhu,Yucan Liu,Qianjin Liu,Lihua Huang
In this study, tartrazine solutions were oxidized using innovative electrochemical advanced oxidation processes (EAOPs) that combined persulfate (PS) activation with electrogenerated H2O2, cathodic reduction and anodic oxidation at Pt and BDD anodes, and graphite cathode in an undivided stirred reactor. For the Pt anode, SO4·−was generated from a reduction reaction at the cathode and a reaction between the PS and electrogenerated H2O2. For the BDD anode, SO4·−was generated from a reduction reaction at the cathode, an oxidation reaction at the anode, and a reaction between PS and electrogenerated H2O2. Among these activation methods, the activation efficiency of PS by electrogenerated H2O2is much better than other methods. The effects of PS concentration up to 36 mM, applied current density between 6 to 15 mA cm−2,and temperatures between 25 to 45 °C were investigated. For the electro-Fenton process with Pt anode (Pt-H2O2/PS process), the best result for oxidizing 250 mg L−1tartrazine solution was obtained with 37.5 mM Na2SO4+ 9.0 mM Na2S2O8, applied current density at 12 mA cm−2and 45 °C, acquiring total color removal after 30 min reaction. For the electro-Fenton process with BDD anode (BDD-H2O2/PS process), the best result for oxidizing 250 mg L−1tartrazine solution was obtained with 25 mM Na2SO4+ 18 mM Na2S2O8, applied current density at 12 mA cm−2and 45 °C, yielding 100% color removal after 30 min reaction. The main oxidizing agents are SO4·−and OH·in the anodic oxidation process with PS and the electro-Fenton process with PS. It is concluded that the additions of PS tremendously improve the oxidation power of electro-Fenton processes with PS, especially the Pt-H2O2/PS process.Keywords:anodic oxidation;electrogenerated H2O2;persulfate;hydroxyl radical;sulfate radical
研究领域:
环境科学
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化学工程
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水处理
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PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2023
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IF 5.8
Shuo Liu,Huiqi Yang,Liguo Zhang,Alberto Bianco,Baojin Ma,Shaohua Ge
The injured bone microenvironment is typically characterized by high-level reactive oxygen species (ROS, mainly H 2 O 2 ) and low content of oxygen. The former can cause cell apoptosis, induce immune imbalance and inflammation, inhibit osteogenic differentiation, and delay bone repair . In this work, barrier membranes made of a combination of hydroxyapatite nanowires (HAp NWs) and polylactic acid (PLA) loaded with manganese dioxide nanosheets (NSs) ( [email protected] ) were prepared to guide bone regeneration (GBR). Due to the catalytic function of MnO 2 , [email protected] membranes are able to effectively decompose H 2 O 2 to generate O 2 . In vitro experiments demonstrated [email protected] membranes inhibited the apoptotic process caused by high level of H 2 O 2 , decreased intracellular oxidative stress , suppressed inflammation-related gene/protein expression, and inhibited macrophage M1 polarization. The nanostructured HAp stimulated osteogenic differentiation, and the loading of MnO 2 NSs played a synergistic effect. In addition, the barrier function of PLA could hamper the epithelium overgrowth, providing the development space for periodontal regeneration. By scavenging H 2 O 2 , generating O 2 , eliminating inflammation, and regulating immune response, [email protected] membranes efficiently promoted bone regeneration. Our results confirmed that composite membranes have great potential for application in GBR, and provide a reference for future design of bone repair materials.
研究领域:
生物材料
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组织工程
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再生医学
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骨科
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Peipei Huang,Qing Chang,Guodong Jiang,Xu Wang,Haipeng Zhu,Qianqian Liu
In this article, a novel metal–organic framework, namely MIL-101(Fe II ), was firstly synthesized via a facile method. In the presence of H 2 O 2 , MIL-101(Fe II ) possesses excellent peroxidase-like activity toward the classical chromogenic substrate, N, N -Diethyl-p-phenylenediamine sulfate salt (DPD). The substitution of Fe 2+ enhances the construction of Fe(II)-oxo nodes and accelerates electrons transfer between DPD and H 2 O 2 , thereby improving the peroxidase-mimicking catalytic activity of MIL-101(Fe II ) nanoenzyme. Additionally, DPD molecules could be adsorbed readily onto the surface of the nanoparticles due to the π–π interaction. The study of Michaelis constant indicates that the MIL-101(Fe II ) exhibits a higher affinity towards DPD (0.16 mM) in contrast to horseradish peroxidase (0.78 mM). In view of the impressive catalytic performance of MIL-101(Fe II ), two reliable monitoring platforms for the rapid detection of H 2 O 2 and glucose were established with extremely low detection limits of 18.04 nM and 0.87 μM in the ranges of 40–5000 nM and 1.2–300 μM, respectively. The study of the catalytic mechanism indicates that DPD oxidation is attributed to the hydroxyl radical (·OH) produced from the decomposition of H 2 O 2 catalyzed by MIL-101(Fe II ). Furthermore, the developed sensor indicates high selectivity and stability and can be effectively appropriate for the detection of H 2 O 2 and glucose in real samples. This work not only provides a novel nanozyme with superior catalytic performance for biological analysis, but also broadens the application field of MIL-101(Fe II ) material.
研究领域:
分析化学
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纳米技术
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生物催化
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生物传感器
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产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
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2022
·
IF 10.633
Junqin Li,Wenhua Yi,Yuze Luo,Ke Yang,Lidan He,Caiyun Xu,Le Deng,Dinggeng He
Nanozyme-based chemodynamic therapy (CDT) has shown tremendous potential in the treatment of bacterial infections. However, the CDT antibacterial efficacy is severely limited by the catalytic activity of nanozymes or the infection microenvironments such as insufficient hydrogen peroxide (H 2 O 2 ) and overexpressed glutathione (GSH). Herein, a versatile hybrid nanozyme (MoS 2 /CuO 2 ) is rationally constructed by simply decorating ultrasmall CuO 2 nanodots onto lamellar MoS 2 platelets of hydrangea-like MoS 2 nanocarrier via a covalent Cu-S bond. The MoS 2 /CuO 2 nanozyme exhibits the peroxidase-mimic activity for catalytically converting H 2 O 2 produced by acid-triggered decomposition of the decorated CuO 2 into hydroxyl radical (•OH). Meanwhile, the MoS 2 /CuO 2 can consume GSH overexpressed in the infection sites via redox reaction mediated by polyvalent transition metal ions (Cu 2+ and Mo 6+ ) for enhanced CDT. More importantly, MoS 2 support can promote the conversion of Cu 2+ to Cu + by a co-catalytic reaction based on the Mo 4+ /Mo 6+ redox couples, and provide photonic hyperthermia (PTT) to augment the peroxidase-mimic activity. The developed MoS 2 /CuO 2 nanozymes possesses a desirable catalytic property, as well as a remarkably improved antibacterial efficiency both in vitro and in vivo . Taken together, this study proposes a synergetic multiple enhancement strategy to successfully construct the versatile hybrid nanozymes for intensive in vivo PTT/CDT dual-mode anti-infective therapy. Statement of significance Chemodynamic therapy (CDT) has shown great potentialities in the treatment of bacterial infections, while its therapeutic efficiency is severely limited by the infection microenvironments such as insufficient hydrogen peroxide (H 2 O 2 ) and overexpressed glutathione (GSH). Here, we rationally construct a hybrid nanozyme (MoS 2 /CuO 2 ) with peroxidase-like activity that can enhance CDT by regulating local microenvironments, that is, simultaneously self-supplying H 2 O 2 and consuming GSH. Importantly, MoS 2 support can promote the conversion of Cu 2+ to Cu + by the Mo 4+ /Mo 6+ redox couples, and provide photonic hyperthermia (PTT) to augment the peroxidase-mimic activity. The developed MoS 2 /CuO 2 shows desirable PTT/CDT dual-mode antibacterial efficacy both in vitro and in vivo . This study proposes a versatile hybrid nanozyme with multiple enhancement effects for intensive in vivo anti-infective therapy.
研究领域:
纳米技术
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生物医学工程
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材料科学
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治疗学
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抗菌研究
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产品关联
DOI
PubMed
高影响因子
Drug Design Development and Therapy
·
2024
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IF 4.7
Yu Li,Guangren Yue,Shuying Yu,Zheng Liu,Yilin Cao,Ximei Wang
Introduction Autologous stem cell transplantation has emerged as a promising strategy for bone repair. However, the osteogenic potential of mesenchymal stem cells derived from diabetic patients is compromised, possibly due to hyperglycemia-induced senescence. The objective of this study was to assess the preconditioning effects of extracellular vesicles derived from H2O2-stimulated adipose-derived stem cells (ADSCs) and non-modified ADSCs on the osteogenic potential of diabetic bone marrow mesenchymal stem cells (BMSCs).Methods Sprague-Dawley (SD) rats were experimentally induced into a diabetic state through a high-fat diet followed by an injection of streptozotocin, and diabetic BMSCs were collected from the bone marrow of these rats. Extracellular vesicles (EVs) were isolated from the conditioned media of ADSCs, with or without hydrogen peroxide (H2O2) preconditioning, using density gradient centrifugation. The effects of H2O2 preconditioning on the morphology, marker expression, and particle size of the EVs were analyzed. Furthermore, the impact of EV-pretreatment on the viability, survivability, migration ability, osteogenesis, cellular senescence, and oxidative stress of diabetic BMSCs was examined. Moreover, the expression of the Nrf2/HO-1 pathway was also assessed to explore the underlying mechanism. Additionally, we transplanted EV-pretreated BMSCs into calvarial defects in diabetic rats to assess their in vivo bone formation and anti-senescence capabilities.Results Our study demonstrated that pretreatment with EVs from ADSCs significantly improved the viability, senescence, and osteogenic differentiation potential of diabetic BMSCs. Moreover, in-vitro experiments revealed that diabetic BMSCs treated with H2O2-activated EVs exhibited increased viability, reduced senescence, and enhanced osteogenic differentiation compared to those treated with non-modified EVs. Furthermore, when transplanted into rat bone defects, diabetic BMSCs treated with H2O2-activated EVs showed improved bone regeneration potential and enhanced anti-senescence function t compared to those treated with non-modified EVs. Both H2O2-activated EVs and non-modified EVs upregulated the expression of the Nrf2/HO-1 pathway in diabetic BMSCs, however, the promoting effect of H2O2-activated EVs was more pronounced than that of non-modified EVs.Conclusion Extracellular vesicles derived from H2O2-preconditioned ADSCs mitigated senescence in diabetic BMSCs and enhanced their bone regenerative functions via the activation of the Nrf2/HO-1 pathway.
研究领域:
干细胞生物学
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再生医学
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细胞衰老
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骨再生
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氧化应激
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）