Stem Cell Research & Therapy
·
2025
·Vol. 16
·期 1
·1-16
·
IF 7.3
Chen Guangzi,Xu Tao,Gao Ran,Liu Wenbin,Li Weigang,Zeng Delu,Li Jian,Fang Xuan,Sheng Gaohong,Zhao Hongqi,Liu Chaoxu
Background Periosteum plays an important role in bone defect repair due to its rich vascular network and cells. However, natural periosteum is difficult to meet clinical requirements due to its low availability. Therefore, it is necessary to develop a tissue engineering strategy of biomimetic periosteum for bone defect repair. Methods Poly-ε-caprolactone/chitosan/Whitlockite electrospun bionic membrane (PCL/CS/WH) was prepared using electrospinning technology, then it was conjugated with an E7 peptide as PCL/CS/WH/E7 bionic membrane. The physical properties of the membranes were evaluated by TEM, FTIR and tensile strength testing. In vitro, LIVE/DEAD staining and Cell Counting Kit-8 assay of bone marrow mesenchymal stem cells (BMSCs) and Endothelial progenitor cells (EPCs) are used to assess the biocompatibility of bionic membranes. Matrigel was applied to evaluate the ability of the different composite nanofibers samples to promote angiogenesis. Mineralized nodule and collagen formation in the BMSCs was detected by alizarin red staining and sirius red staining respectively. The expression of osteogenesis related genes and angiogenesis associated genes were detected using quantitative real-time polymerase chain reaction (qRT-PCR). In vivo, the ability of PCL/CS/WH/E7 membrane to promote bone regeneration and angiogenesis was assessed by Micro-CT and associated staining. Result The addition of chitosan (CS) and E7 peptide (E7) enhanced the hydrophilicity and cytocompatibility of pure PCL membranes. Additionally, CS, E7 and Mg 2+ released from Whitlockite (WH) had a synergistic effect to promote angiogenesis and osteogenic differentiation. Three weeks after implantation, the membrane successfully bridged the bone defect and significantly promoted the formation of new bone and blood vessels. Conclusion The PCL/CS/WH/E7 membrane to achieve efficient repair of bone tissue and enriches clinical solutions for the treatment of critical bone defects.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Sherif A. El-Khodary,A.A. Menazea,Sayeda Abdelrazek Abdelhamid,Mohamed Khalaf
Skin wound healing is undoubtedly a complex and challenging process. Creating innovative bio-nanocomposites for skin care and wound healing/dressing applications are currently an urgent need. In this context, a mixture of chitosan (CS) and MnO 2 nanowires (NWs), as potential wound dressing nano-film, are systematically investigated for optical, electrical, and antibacterial applications. The crystallinity of the materials was investigated through XRD, while FTIR spectroscopy was used to examine the interactions between the CS films and the MnO 2 nanofiller. The resulting nanocomposite membranes exhibit superior swelling capacity than that of the pure CS membrane. Additionally, the thermal stability of the films was evaluated, demonstrating the highest stability of composite samples. The optical properties, including bandgap energies and refractive indices, can be adjusted by tuning the content of MnO 2 . Remarkably, the refractive index dropped from 3.76 in pure CS film to 3.4 in the highest fraction of MnO 2 (CS-4 wt% MnO 2 ). The impedance analysis was further improved for the (CS-4 wt% MnO 2 ) sample, posing lower interfacial resistance. A comprehensive investigation of the antibacterial performance of the prepared films was carried out against five bacteria types. The pure CS film exhibited mostly inhibitory effects against Gram-positive bacteria (i.e., B. subtilis , and S. aureus ) between the 19 and 14 mm range, while the Gram-negative bacteria (i.e., E. coli , and P. aeruginosa ) showed activity between the 19 and 20 mm range, with an effect on A. niger and C. albicans 17 and 22 mm. However, the addition of MnO 2 has significantly increased its antimicrobial activity, which exhibited mostly inhibitory effects against Gram-positive bacteria between the 16 and 22 mm range, while the Gram-negative bacteria showed activity between the 16 and 24 mm range, with an effect on A. niger and C. albicans from 13 to 23 mm. Overall, the prepared CS films demonstrate great potential for multifunctional applications, including antibacterial, wound healing, and optoelectronics.
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Journal of Coatings Technology and Research
·
2025
·
IF 2.8
Li Senchi,Zhang Na,Peng Lai,Zhang Yang
The degradation and erosion of wood and its products caused by microorganisms remains a persistent challenge, which leads to significant economic and property losses and poses a potential health threat to users due to the presence of pathogenic microorganisms. In light of the recent COVID-19 pandemic, this issue has become increasingly urgent to address in modern society due to the increasing focus on private health. In this work, the carboxymethyl chitosan nano-silver (CMCS-Ag) was prepared through a microwave-assisted method, where the CMCS-Ag was ultrasonically blended with waterborne paint to obtain a waterborne antimicrobial wood coatings. Compared with commercial nano-silver with the same particle size, due to the unique system, the CMCS-Ag exhibited superior antibacterial efficacy and lower Ag + release. CMCS-Ag exhibited effective dispersion within waterborne coatings, leading to a significant improvement in both the mechanical and antimicrobial performance of the coatings. With a CMCS-Ag content of 10 wt%, the coating films exhibit high elastic modulus, tensile strength and shore hardness, 78%, 33% and 69% higher than the control, respectively. Moreover, antimicrobial tests confirm that CMCS-Ag wood coatings inhibit Escherichia coli (24 h sterilization rate: 99.99%), Aspergillus niger (28 days without erosion), and soil decay fungi (56 days undecayed), while minimizing wood product appearance deterioration and mass loss from microbial erosion. These findings not only provide valuable insights into enhancing the antimicrobial of wood and its products but also reduce possibilities for people exposed to pathogens.
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LANGMUIR
·
2025
·
IF 3.9
Yuting Li,Mengyi Liu,Feng Hu,Fangjiu He,Xiaoyang Li,Hongbing Deng,Yumin Du,Xiaowen Shi
The exceptional multiple responsiveness of hydrogels has garnered significant attention for their potential in storing dynamic information. Self-erasing information observable by the naked eye provides an alternative method for data security. Herein, we report a polydopamine (PDA)-modified chitosan (CS) hydrogel that automatically reveals information without external stimuli. Information is embedded in a CS hydrogel containing horseradish peroxidase through an electrical writing process, creating localized high pH regions that trigger rapid enzymatic polymerization of dopamine (DA) into dark PDA. However, unwritten regions experience slower self-oxidation kinetics in air. This mismatched DA oxidation leads to information revelation and time-dependent erasure. The information lifetime can be precisely regulated by the number of electrical writing cycles and the magnitude of the electric current, enabling complex information decryption and allowing for the recognition of valid and invalid signals. These results offer valuable insights into fabricating and applying patterned hydrogels for information storage.
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Advanced Healthcare Materials
·
2025
·
IF 9.6
Yueting Li,Siting Du,Yanzhen Feng,Xiaopeng Zhou,Kaiting Zhao,Jianfeng Wang,Quan Zhou,Yaru Shi,Ayesha Younas,Yonghui Shen,Shuanghu Wang
Bacterial infections pose a significant challenge to global health, as conventional treatments frequently cause side effects and resistance. Phototherapy, encompassing photothermal therapy (PTT) and photodynamic therapy (PDT), emerges as a viable alternative, eradicating bacteria through heat and reactive oxygen species (ROS). However, excessive heat and ROS can exacerbate local inflammation and comlicate healing. To address these challenges, we developed a novel chitosan-based nanoparticle (NPs) encapsulating S-nitrosoglutathione (GSNO) and indocyanine green (ICG), termed as GSNO/ICG@NPs. This biocompatible nanosystem integrates the triple synergistic effects of nitric oxide (NO) release, PTT, and PDT. The stable and uniformly shaped GSNO/ICG@NPs were synthesized via a charge-driven self-assembly method with efficient loading of GSNO and ICG. Upon irradiation with an 808 nm near-infrared (NIR) laser, GSNO/ICG@NPs rapidly generate heat and 1 O 2 , effectively eliminating bacteria. Simultaneously, the photothermal effect releases NO from GSNO, modulates inflammation, promotes neovascularization, and supports tissue repair. In vitro and in vivo studies demonstrated that GSNO/ICG@NPs significantly enhance antibacterial activity, reduce inflammation, and promote angiogenesis. It effectively eradicated biofilms and accelerated wound healing in a Staphylococcus aureus -infected mouse skin model. These findings highlight GSNO/ICG@NPs as a promising alternative to antibiotics for treating infected wounds via synergistic therapy involving balanced NO release, PTT, and PDT.
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Journal of Water Process Engineering
·
2024
·
IF 6.3
Yuning Zhang,Kexin Tao,He Gui,Zhiyuan Chen,Yao Wang,Yanxin Wang,Matt J. Kipper,Jianguo Tang,Linjun Huang
Graphene oxide (GO) -based membranes have attracted attention in dye wastewater treatment in recent years. However, water permeance and methyl orange (MO) rejection need improvement. In this work, a simple method was used to incorporate hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) to enhance the charge of the membrane. An optimized HTCC-GO composite membrane (HGM membrane) containing a 2: 1 ratio of HTCC to GO membrane significantly improved MO rejection (96.94 %), and achieved high rejection of the other three dye molecules studied in this work (>93 %), and also realized a two-fold higher water permeance (28.09 L m −2 h −1 bar −1 ) compared to a pure GO membrane. Furthermore, the hydrophilic and antimicrobial properties of HGM membranes are enhanced as a result of quaternary ammonium groups in HTCC. Moreover, the acid-base stability of HGM membranes was also improved in comparison to the original GO membrane. This work provides a reference for the modification of GO-based membranes and their application in the direction of practical treatment of dye wastewater.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Huiyun Zhang,Xinling Li,Weishi Shan,Jingkun Guo,Xuyang Hu,Huaibin Kang
This study aimed to investigate the impact of substituting pork back fat with Pickering emulsion (PE) stabilized by soybean protein isolate-chitosan (SPI-CH) nanoparticles on the quality of pork batters. Cooking loss, rheological properties, water distribution, microstructures, texture, color, and oxidative stability of pork batters were evaluated. The results revealed that replacing fat with PE led to a reduction in fat content and an increase in moisture content, as well as higher L * and b * values ( P < 0.05). Furthermore, the substitution resulted in improved gel performance by enhancing rheological properties and texture, reducing water mobility and cooking loss, as well as effectively inhibiting lipid and protein oxidation in pork batters. However, no significant differences were observed in sensory acceptability. The findings suggest that using SPI-CH nanoparticles as a stabilizer for PE presents significant potential as a nutritionally improved fat alternative in meat products, offering enhanced functional characteristics.
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Journal of Food Measurement and Characterization
·
2026
·
IF 3.6
Lai Yuanrong,Shao Yimin,Jiang Bokai,Fan Kehao,Sun Ningjing,Qin Zhiyong
Bio-based composite films offer opportunities for postharvest fruit protection, although their practical performance requires careful evaluation. Dialdehyde cellulose nanocrystals (DACNC) and vanillin (VA) were incorporated into a gelatin/chitosan (Gel/CS) matrix. Structural analyses indicated changes in chemical environments consistent with possible interfacial interactions, but did not independently confirm Schiff-base formation. The formulation containing DACNC and VA at a mass ratio of 1:3 (CGDV1) was selected for exploratory blueberry preservation evaluation. CGDV1 showed a tensile strength of 34.72 MPa and an elongation at break of 24.74%, together with enhanced ultraviolet shielding and composition-dependent water-related properties. The CGDV1 film-forming solution showed radical-scavenging activity, whereas the antioxidant performance of the final dried film was not directly evaluated. Representative antibacterial plates showed visually lower colony density for CGDV1, but these observations were qualitative and lacked independent biological replication. The CGDV1 film-forming solution maintained L929 cell viability above 80% at solid-equivalent concentrations of 15.6–125 µg/mL. During storage at 26 ℃, CGDV1-treated blueberries showed comparatively better visual appearance and a lower descriptive weight-loss trend than the water-treated control. These preservation observations were preliminary because independent biological replication was lacking and storage relative humidity was neither controlled nor monitored. These findings support CGDV1 as a candidate bio-based coating, with further validation needed through independently replicated storage experiments under controlled conditions.
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PLoS One
·
2026
·Vol. 21
·期 10
·e0359159
·
IF 2.8
Zhuyun Liu,Weilu Guo,Lei Wang,Pengcheng Zhao,Shanshan Yang,Donghua Zhai,Baochao Fan,Lizhong Wang,Bin Li
Background
Subunit vaccines offer excellent safety profiles but often suffer from limited immunogenicity, requiring potent adjuvants to elicit robust protection. We hypothesized that combining a positively charged biopolymer, trimethyl chitosan (TMC), with a nanoemulsion (NE) would create a synergistic delivery system to enhance immune responses. This study aimed to formulate a TMC-loaded nanoemulsion (TMC-NE) and evaluate its potential as a safe, effective adjuvant for subunit vaccines.
Methods
TMC was synthesized via the quaternization of chitosan. Its modification and cyto-compatibility were verified by FT-IR, Zeta potential analysis, and cytotoxicity assays. Based on the pseudo-ternary phase diagram, a stable oil-in-water blank nanoemulsion (NE) was prepared. Subsequently, TMC-loaded nanoemulsion (TMC-NE) was prepared with TMC aqueous solution as the aqueous phase. The formulation was characterized for its droplet size, polydispersity index (PDI), and viscosity, and its safety in vivo was evaluated in mice. Using ovalbumin (OVA) as the model antigen, the immune adjuvant activity of TMC-NE was evaluated in mice by intramuscular injection, and compared with the NE and a commercial similar adjuvant M902. Humoral and cellular immune responses were analyzed by measuring serum IgG antibody levels and splenic lymphocyte proliferation, as well as the expression of cytokines IL-4 and IFN-γ.
Results
Chitosan was successfully quaternized, yielding TMC with low cytotoxicity. The formulated TMC-NE was clear and transparent, with a mean droplet size of 25.41 ± 2.13 nm, a PDI of 0.231, and suitable viscosity. In vivo, TMC-NE showed no significant tissue toxicity. Immunization studies revealed that TMC-NE stimulated IgG antibody levels comparable to the M902 adjuvant and significantly higher than the blank NE. Furthermore, TMC-NE significantly increased the expression of IL-4 and IFN-γ and promoted lymphocyte proliferation and T lymphocyte levels, indicating the induction of both humoral and cellular immune responses.
Conclusions
The TMC-loaded nanoemulsion (TMC-NE) is characterized as a safe and effective antigen delivery system with potent immune adjuvant properties. These findings strongly support its prospective application as a novel vaccine adjuvant.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Ziqiang Zhou,Xuejuan Zhang,Lijun Xu,Huangjie Lu,Yuying Chen,Chuanbin Wu,Ping Hu
Traditional wound dressings are not able to provide ideal environment for diabetic wounds surface thus hampered the regrowth of fresh tissues. In this study, we designed a novel in situ forming hydrogel and used it as wound dressing material. Carboxymethyl chitosan (CMCS) and oxidized hyaluronic acid (OHA) were selected to construct a pH-responsive and self-healing hydrogel system via Schiff base reaction. Taurine (Tau) with anti-inflammatory property was loaded in the hydrogel through the aforementioned reaction. Under the slightly acidic environment of the diabetic wound site, a responsive release of taurine molecules speeded up the transfer of the taurine into the wound. The physiochemical properties of the prepared CMCS-OHA-Tau hydrogel were characterized. The CMCS-OHA-Tau hydrogel showed good biocompatibility, enhancement of cell migration and inhibited production of inflammatory cytokines.Subsequently, the hydrogel was applied on the wounds of diabetic rats and its boosted efficacy for wound recovery was confirmed.
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CELL BIOCHEMISTRY AND FUNCTION
·
2025
·Vol. 43
·期 12
·e70143
·
IF 2.7
Hao Song,Dongxu Tang,Buyu Wang,Yanfei Luo,Changzheng Yan,Shiqiang Ruan
To explore the effects of a collagen-binding domains-bone morphogenetic protein 2 (CBD-BMP-2) scaffold on repairing cartilage defects. A chitosan-collagen scaffold was prepared and its physicochemical properties were evaluated. A plasmid encoding the CBD and BMP-2 was constructed, a CBD-BMP-2 composite scaffold was prepared, and the controlled release of BMP-2 was assessed in vitro. Knee cartilage defect model rabbits were divided into the control, CBD, and CBD-BMP-2 groups ( n = 9). Gross observation, MRI, staining, and IHC were performed to evaluate the cartilage repair outcomes. CBD-BMP-2 composite scaffold provided sufficient support and promoting cell attachment and proliferation. It consistently released BMP-2 for over 30 days in vitro. It significantly enhanced cartilage regeneration and repair. After surgery, cartilage growth in the CBD-BMP-2 group was superior to the control and CBD groups. The CBD-BMP-2 group demonstrated better coverage of new cartilage tissue. In the CBD-BMP-2 group, new cartilage tissue was tightly structured and cells were neatly arranged, and there were a rich cartilage matrix and dense collagen fibers. Type II collagen expression was significantly increased in the CBD-BMP-2 group. CBD-BMP-2 composite scaffold has excellent physicochemical properties and controlled-release functionality, providing superior repair effects for rabbit knee joint cartilage defects. Summary Cartilage injuries are difficult to treat due to limited self - healing capacity. Current treatments offer only temporary relief. The CBD - BMP - 2 slow - release composite scaffold combines the ability of BMP - 2 to promote cartilage regeneration with the collagen - binding property of CBD. Our study shows it has excellent mechanical properties and controlled - release functionality, providing superior repair for rabbit knee cartilage defects, offering a novel solution for cartilage injury repair.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Guoren Li,Jiehong Lei,Man He,Jinze Yu,Tingting Song,Hui Zhu
This study achieved the directed functionalization of 3-aminopyrazole onto the interface of chitosan aerogel via the Schiff base reaction, successfully preparing a porous, lightweight, lamellar pyrazole-functionalized chitosan aerogel material (Pyrazole@CSA-5). This material demonstrates specific and efficient capture of iodine vapor and methyl iodide under strong acidic, high-humidity, and low-concentration dynamic conditions. At 75 °C, Pyrazole@CSA-5 achieved capture capacities of 5255 mg/g for iodine vapor and 1060 mg/g for methyl iodide. Under dynamic low-concentration conditions, the material maximum capture capacities for iodine vapor (8.75 ppm) and methyl iodide (18.6 ppm) were 1658 mg/g and 410 mg/g, respectively, significantly outperforming similar adsorbents. Mechanism characterization revealed that Pyrazole@CSA-5's adsorption of iodine vapor and methyl iodide is attributed to electron transfer with imine groups and nitrogen methylation reactions with imine groups, respectively. DFT calculations revealed adsorption energies of −0.70 eV and −0.57 eV for iodine, −0.70 eV and −0.61 eV for methyl iodide at the two distinct imine sites, confirming the material's outstanding adsorption performance toward both iodine vapor and methyl iodide. After treatment with the strong acid at pH = 1, Pyrazole@CSA-5 exhibited the iodine vapor capture capacity of 4125 mg/g. Under 80 % humidity conditions, its capture capacities for iodine vapor and methyl iodide were 3735 mg/g and 1007 mg/g, respectively, maintaining good capture performance. Thus, Pyrazole@CSA-5 demonstrates effective specific capture of iodine vapor and methyl iodide under complex conditions, exhibiting strong potential for practical application.
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