SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2019
·
IF 2.931
Kui Chen,Weixia Qing,Weiping Hu,Minghua Lu,Yong Wang,Xiuhua Liu
In this work, we reported an economical plant-based hydrothermal method for one-pot green synthesis of water-soluble carbon dots (Tea-CDs) by using waste tea extract as a carbon source. The synthesized Tea-CDs were characterized by UV–visible, fluorescence, FT-IR, TEM, XPS and XRD. The Tea-CDs were found to remove hydroxyl and superoxide anion radical in vitro. In addition, the Tea-CDs exhibited bright blue fluorescence under UV-light (λ ex = 365 nm), and the fluorescence could be effectively quenched by CrO 4 2− and Fe 3+ ions. Meanwhile, the fluorescence of Tea-CDs-CrO 4 2− and Tea-CDs-Fe 3+ systems could be again easily recovered by ascorbic acid (AA) and L-cysteine (L-Cys). As an on-off-on fluorescent nano-sensor of the Tea-CDs, the sensitive detection of CrO 4 2− , Fe 3+ , AA and L-Cys were all performed, showing that the good linear relationships between fluorescence intensity of Tea-CDs and concentration of all testing samples. Finally, the sensors successfully detected CrO 4 2− , Fe 3+ , AA and L-Cys in commercially available real samples with satisfactory recovery ranges. The prepared sensors offer distinct advantages including low cost, simple handling, good sensitivity and high selectivity.
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高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Muhammad Saqib,Shahida Bashir,Haijuan Li,Chuanping Li,Shanshan Wang,Yongdong Jin
Developing rapid, specific, and low-cost electrogenerated chemiluminescence (ECL) systems remains a compelling goal for many applications including environmental analysis, clinical diagnostics, and food safety. In this study, N-hydroxysuccinimide (NHS) esters, especially N-hydroxysulfosuccinimide (NHSS), was exploited for the first time as an efficient coreactant for anodic Ru(bpy)32+ ECL in neutral medium. Among the three tested NHS esters (NHS, NHSS, NHPI), the NHSS exhibited much higher ECL intensity and stability than the sulfonate-free esters, suggesting the essential role of both the N-hydroxy (NOH) and sulfonate (SO3H) moieties in the developed Ru(bpy)32+ ECL system. The ECL generation process is rather straightforward, not requiring time-consuming fabrication/modification steps of electrodes. The anodic Ru(bpy)32+–NHSS system achieved ECL detections with high selectivity and sensitivity toward l-proline (LOD = 50 nM) and Hg2+ (LOD = 10 nM), with a linear range of 0.5–200 μM and 0.1–25 μM, respectively. The method showed good recoveries (95.2–104.8%) for l-proline and Hg2+ detection in real samples. This study is a successful step toward the development of new coreactants and may open new avenues for the applications of N-hydroxy compounds in ECL sensing.
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Journal of Food Measurement and Characterization
·
2018
·Vol. 12
·期 4
·2475-2483
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IF 1.181
Zhang Yao,Sang Jie,Chen Fang-fang,Sang Jun,Li Cui-qin
This study aimed to develop a green approach for extraction and determination of anthocyanins from Hibiscus sabdariffa calyx using a hydroxypropyl- β -cyclodextrin (HP β -CD)-assisted extraction technique coupled with HPLC-DAD analysis and to investigate the effects of gallic/ferulic/caffeic acids on the stability of H. sabdariffa anthocyanins in beverages. HP β -CD-assisted extraction of total anthocyanins from H. sabdariffa calyx was optimized using a Box–Behnken design-response surface methodology, and extraction at 54 °C for 53 min using HP β -CD aqueous solution (3.7 g/100 mL) gave the maximum extraction yield of total anthocyanins (4.82 ± 0.13 mg/g). A work-safe and rapid HPLC-DAD method was developed for analysing H. sabdariffa anthocyanins using ethanol and a tartaric acid aqueous solution as mobile phases, and the main anthocyanin peaks were determined as delphinidin-3- O -sambubioside and cyanidin-3- O -sambubioside using a two-dimensional HPLC-DAD/ESI-MS method. Method validation showed that the developed method was accurate, stable and reliable for the analysis of H. sabdariffa anthocyanins. Additionally, the degradation of delphinidin-3- O -sambubioside, cyanidin-3- O -sambubioside and total anthocyanins from H. sabdariffa calyx ideally followed a first-order model ( R 2 > 0.98) in beverages alone or with additional phenolic acids. Gallic acid improved the stability of H. sabdariffa anthocyanins better than ferulic and caffeic acids, and adding/increasing ferulic and caffeic acids accelerated anthocyanin degradation.
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POLYMERS FOR ADVANCED TECHNOLOGIES
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2019
·Vol. 30
·期 11
·2846-2859
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IF 2.162
Li Yang,Weijun Zhen
Phosphorylated graphene oxide (PGO) was prepared by using phosphoric acid as functional reagent, and PGO was grafted with poly(L-lactide) (PGO-PLLA) by ring-opening polymerization of L-lactide as monomer under nano-ZnO catalyst. The results of the orthogonal analysis showed the optimum reaction conditions to be as follows: the reaction temperature of 170°C, reaction time of 14 hours, the mass ratio of PGO of 10 wt%, and the mass of nano-ZnO of 1 wt%. PGO-PLLA was characterized by fourier transform infrared spectroscopy, gel permeation chromatography, and X-ray photoelectron spectroscopy, which demonstrated that the PLLA molecular chains were successfully grafted onto the surface of PGO. Poly (lactic acid)/PGO-PLLA nanocomposites (PLA/PGO-PLLA) were prepared by melt intercalation. Mechanical test and fracture scanning electron microscopy showed that PGO-PLLA (0.3 wt%) improved impact strength of PLA by 52.19%, which resulted in ductile fractures surface of PLA/PGO-PLLA. Microcalorimetry and thermal degradation kinetics proved that PGO-PLLA improved the thermal stability of PLA. Polarized optical microscopy and differential scanning calorimetry confirmed that PGO-PLLA increased crystallization rate and spherulite kernel density of PLA, and crystallinity of PLA/PGO-PLLA reached to 22.05%. Rheological behavior proved that PGO-PLLA increased the self-lubricity of PLA. Enzymatic degradation results illustrated that PGO-PLLA had some inhibition for the biodegradability of PLA based nanocomposites.
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RSC Advances
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2017
·Vol. 7
·期 83
·52661-52671
·
IF 3.108
Zhihong Yao,Shishi Li,Zifei Qin,Xiaodan Hong,Yi Dai,Baojian Wu,Wencai Ye,Frank J. Gonzalez,Xinsheng Yao
Norbakuchinic acid (NBKA) is the most abundant metabolite of bakuchiol (a hepatotoxicity and nephrotoxicity component in Psoralea corylifolia L.) in plasma and urine. The present study aimed to identify human UDP-glucuronosyltransferase (UGT) isoforms involved in NBKA glucuronidation, and to assess the inhibition potential of NBKA against recombinant UGTs. NBKA glucuronidation was determined using human liver microsomes (HLM) and human intestine microsomes (HIM) as well as expressed UGTs. The mechanisms of NBKA-UGT interactions were explored through kinetic characterization and modeling. As a result, two glucuronides (G1 and G2) were detected by HLM and HIM. G2 was dominantly produced with CLint values of 3.21 and 0.96 μL min−1 mg−1 in HLM and HIM, respectively. Furthermore, based on the relative activity factor (RAF) approach, UGT1A9 and 2B7 represented 5.98 and 46.16% of G2 in HLM, respectively. In addition, G2 was both significantly correlated with propofol-glucuronidation (r = 0.681, p = 0.015) and AZT-glucuronidation (r = 0.832, p = 0.0008). Also, NBKA glucuronidation showed marked species differences. Moreover, NBKA displayed broad-spectrum inhibition against human UGTs. Kinetic analyses showed that inhibition of these UGTs followed competitive (UGT1A1), mixed (UGT1A3, 1A6, 1A10, 2B7, 2B15 and 2B17) and non-competitive (UGT1A4, 1A7, 1A8 and 1A9) mechanisms, with Ki values ranging from 2.48 to 20.31 μM. Among them, NBKA exhibited moderate inhibitory effects against UGT1A1, 1A3, 1A4, 1A7, 1A8, 2B15 and 2B17 (both IC50 and Ki values lower than 8 μM). In contrast, the effect of NBKA was activating for UGT2B10. Taken altogether, these findings together demonstrate that NBKA has a potent and broad-spectrum inhibitory effect against most human UGTs and thus suggest that much caution should be exercised when high-dose BK is co-administered with UGT substrates.
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MOLECULES
·
2024
·Vol. 29
·期 5
·1142
·
IF 4.2
Xin Wang,Jiatao Guo,Siqi Zang,Baodong Liu,Yuhuan Wu
Matteuccia struthiopterisis one of the most globally consumed edible ferns and widely used in folk medicine. Reports mainly focus on young fronds and the rhizome which are common edible medicinal parts. However, there are few detailed reports on other parts. Therefore, the volatile components of different parts based on HS-SPME-GC-MS were identified, and total flavonoid contents, antioxidant activities and acetylcholinesterase inhibitory activities were compared in order to reveal the difference of volatile components and potential medicinal value of different parts. The results showed that total flavonoid contents, antioxidant activities and volatile components of different parts were obviously different. The crozier exhibited the strongest antioxidant activities, but only underground parts exhibited a dose-dependent inhibition potential against AChE. Common volatile compounds were furfural and 2-furancarboxaldehyde, 5-methyl-. In addition, it was found that some volatile components from adventitious root, trophophyll, sporophyll and petiole were important ingredients in food, cosmetics, industrial manufacturing and pharmaceutical applications.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Yan Du,Yu-lang Chen,Ying Zhang,Yan-lin Zhao,Zhong Huang,Peng Jin,Shuai Ji,Dao-Quan Tang
Ethnopharmacological relevance Mulberry ( Morus alba L.) leaf is a well-known herbal medicine in China for thousands of years. Mulberry leaf can regulate arachidonic acid (ARA) metabolism disorder in obesity and type 2 diabetes mellitus. However, the active ingredients involved in this process are still unclear. Aim of study To explore the potential active ingredients in mulberry leaf against ARA metabolism disorder. Materials and methods In this research, an efficient method combining affinity ultrafiltration, molecular docking, and network pharmacology was developed and applied to explore cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) inhibitors from mulberry leaf. Results A total 17 potential inhibitors were screened by affinity ultrafiltration assay and identified by high resolution mass spectrometry. In addition, 8 bioactive ingredients were obtained after re-evaluated by molecular docking and network pharmacology, and their inhibitory activities on COX-2 and 5-LOX were confirmed by in vitro inhibitory assays. The results of cell experiments showed that the expressions of COX-2 and 5-LOX were significantly suppressed by neochlorogenic acid, rutin, isoquercetin, and (−)-syringaresinol-4-O-glucoside. Conclusion Neochlorogenic acid, rutin, isoquercetin, and (−)-syringaresinol-4-O-glucoside may be the potential material basis of mulberry leaf in the regulation of ARA metabolism of obesity and type 2 diabetes mellitus.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Cui Li,Zhaotian Ba,Bingchen Wu,Xinxin Shi,Hui Liu,Lili Ma,Xin Wen,Pingan Song
The design of environmentally friendly flame retardants to achieve the balanced flame retardancy and mechanical properties of poly(L-lactic acid) (PLA) is a critical challenge. Herein, inspired by the micropapillae adorned with hydrophobic wax crystals structure of lotus leaf, Mg(OH) 2 /Co-MOF/DOPO hybrids (MH@MOF- g -DOPO) were successfully constructed through in-situ growth of Co-MOF and chemical grafting of DOPO. Thermogravimetric analysis (TGA) revealed that the Co-MOF content in MH@MOF was approximately 27.3 wt%, while the grafted DOPO in MH@MOF- g -DOPO was about 19.5 wt%. Owing to the unique microstructure, the water contact angle of MH@MOF- g -DOPO reached to 113.7°, implying its hydrophobic surface. Subsequently, the as-synthesized MH@MOF- g -DOPO was used to prepare PLA composites, which exhibited significantly improved flame retardancy, as indicated by the LOI value of 30.2 %, UL-94 V0 rating, and the reduction on PHRR by 70.1 %. The flame retardant mechanism was attributed to the synergistic effects of multiple flame retardant units (MH, Co-MOF and P) in MH@MOF- g -DOPO hybrids. Furthermore, the flame retarded PLA/20MH@MOF- g -DOPO achieved the brittle-tough transformation via uniaxial pre-stretch process, thus displayed outstanding mechanical properties (good stiffness and excellent toughness). Notably, compared to the pristine undraw PLA, the Youngꞌs modulus, tensile strength and the elongation at break PLA/20MH@MOF- g -DOPO increased by 35 %, 38 % and 1957 %, respectively. This work proposes an innovative and efficient method to achieve the excellent balance on flame retardancy and mechanical properties of PLA composites, benefiting to promoting their real-world applications as functional polymer materials.
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International Journal of Polymeric Materials and Polymeric Biomaterials
·
2017
·
IF 1.515
Qi Jia,Yiran Xia,Shengnan Yin,Zhaosheng Hou,Ruxia Wu
This work describes the preparation, characterization, mechanical properties, in vitro degradability, and cytocompatibility of two aliphatic segmented polyesterurethanes (SPEU-II, SPEU-III) based on poly(ε-caprolactone-co-L-lactide) and aliphatic urethane diisocyanate chain extenders. Two novel chain extenders containing two and four urethane groups, respectively, were synthesized and used in SPEU formulation. The influences of well-defined hard segment length on the degradability and mechanical properties of SPEU films were researched. The results of differential scanning calorimeter and X-ray powder diffraction showed that the long well-defined hard segments improved the phase separation of soft and hard segments, which led to satisfactory mechanical properties of SPEU films (ultimate stress: 24.8–35.4 MPa; strain at break: 601–669%). And the initial modulus increased from 31.9 MPa (SPEU-II) to 41.7 MPa (SPEU-III) with the increasing length of hard segments. SPEU-III with longer well-defined hard segment containing more urethane groups exhibited slower hydrolytic degradation rate (∼67 days) and lower water absorption (∼4 wt%) than SPEU-II. The results could be due to the formation of more hydrogen bonds among the hard segments and between the soft and hard segments, which strengthened the interchain attraction and gave a more compact network structure. The SPEU films showed similar thermostability, indicating that the length of hard segments marginally affects the thermostability. Cytotoxicity test of film extracts was conducted using L929 mouse fibroblasts, and the relative growth rate exceeded 90% after incubation for 24, 48, and 72 h, showing excellent cytocompatibility.
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GREEN CHEMISTRY
·
2014
·Vol. 16
·期 4
·2234-2241
·
IF 6.852
Qiaohong Peng,Khalid Mahmood,Ying Wu,Leli Wang,Yanyan Liang,Jianan Shen,Zhengping Liu
Biodegradable copolymers of L-lactic acid and ε-caprolactone [P(LLA-CL)s] were prepared in sulfonic acid-functionalized Brønsted acidic ionic liquids (SFBAILs) with very different anions and cations. SFBAILs work as both solvents and catalysts. The copolymerization process was proved to be a combination of polycondensation and simultaneous transesterification after a fast ring-opening polymerization of ε-caprolactone through a kinetic study. Copolymers with different monomer ratios were obtained. We found that the anions rather than the cations of SFBAILs have a great impact on the polymerizations. In addition, SFBAILs can be simply recovered by phase separation and recycled without decreasing the efficiency.
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Analytical Methods
·
2014
·Vol. 6
·期 13
·4845-4852
·
IF 1.938
Qi Yun,Qingwang Liu,Cunyong He,Xiaohua Ma,Xiaoli Gao,Amer Talbi,Jianping Zhou
Nigella glandulifera Freyn et Sint seeds (NG) as well as Nigella sativa L. seeds (NS) have been employed as folk remedies for centuries. However, little work has been done on their quality control. In order to assess the quality and guarantee therapeutic effects, the chemical constituents of the two herbs were extensively characterized by ultra-performance liquid chromatography coupled with electrospray time-of-flight mass spectrometry (UPLC-Q-TOF/MS), and a simple, practical, and reliable HPLC fingerprinting method was established and applied to determination of 20 batches of samples. With their chromatographic fingerprints, the two species were successfully differentiated by pattern recognition methods, namely, principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA). Results from this study show that the proposed analytical methods can be applied to the quality control of the seeds and have high potential to ensure their safety and efficacy.
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BIOMACROMOLECULES
·
2014
·
IF 5.788
Qiuming Liu,Hongshi Zhu,Jingya Qin,Haiqing Dong,Jianzhong Du
Presented in this article is the preparation of a new theranostic vesicle which exhibits excellent in vitro and in vivo T1 magnetic resonance (MR) imaging contrast effect and good anticancer drug delivery ability. The theranostic vesicle has been easily prepared based on an amphiphilic biocompatible and biodegradable dibock copolymer, poly(ethylene glycol)-block-poly(l-lactic-co-glycolic acid) (PEG-b-PLGA) and bovine serum albumin-gadolinium (BSA-Gd) complexes. Dynamic light scattering (DLS), transmission electron microscopy (TEM), UV–vis spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) measurements confirmed the formation and physiological stability of BSA-Gd@PEG-b-PLGA vesicles. Furthermore, the in vitro and in vivo MR imaging experiments revealed their excellent T1-weighted MR imaging function. Red blood cell hemolysis and cytotoxicity experiments confirmed their good blood compatibility and low cytotoxicity. Doxorubicin (DOX) loading and release experiments indicated a more retarded release rate of DOX in those theranostic vesicles than sole PEG-b-PLGA nanoparticles without BSA. Overall, this new biocompatible and biodegradable vesicle shows promising potential in theranostic applications.
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