International Journal of Nanomedicine
·
2025
·
IF 6.5
Jia Zhang,Xiaotuan Zhang,Zhuocheng Yao,Juan Pan,Jianzhong Ye,Ping Xia,Tieli Zhou,Jianming Cao
Introduction Carbapenem-resistant gram-negative bacteria (CR-GNB) pose a significant threat to public health and require immediate attention. The development of novel antibacterial agents against CR-GNB has become an urgent priority, and nanomaterials offer promising solutions due to their unique properties. This study introduces 5-amino-2-mercaptobenzimidazole (5-A-2MBI) functionalized gold nanoparticles (5-A-2MBI_Au NPs) and evaluates their antibacterial activity against CR-GNB.Methods The 5-A-2MBI_Au NPs was synthesized using a one-pot method. Its biocompatibility, bactericidal properties, and mechanisms of action were systematically characterized through in vivo and in vitro toxicity tests, antimicrobial susceptibility testing, live/dead staining, membrane permeability and reactive oxygen species (ROS) generation assays, as well as transcriptomic analysis.Results The results of this study demonstrate that 5-A-2MBI_Au NPs exhibit excellent antibacterial efficacy against carbapenem-resistant gram-negative bacteria with various resistance mechanisms, with a minimum inhibitory concentration (MIC) of 2 μg/mL. In vivo experiments further confirmed that 5-A-2MBI_Au NPs not only possess effective bactericidal activity but also exhibit satisfactory biocompatibility. Mechanistic studies revealed that 5-A-2MBI_Au NPs enhance bacterial membrane permeability, increase the generation of reactive oxygen species, and disrupt intracellular oxidative stress and succinate synthesis, thereby conferring potent antibacterial activity. This study results demonstrate that 5-A-2MBI_Au NPs exhibit notable antibacterial efficacy against CR-GNB, with a minimum inhibitory concentration of 2 μg/mL. The antibacterial mechanism involves enhanced membrane permeability, increased reactive oxygen species production, and interference with intracellular oxidative stress and succinate synthesis. These mechanisms collectively contribute to the potent antibacterial activity of 5-A-2MBI_Au NPs against CR-GNB.Discussion 5-A-2MBI_Au NPs are a novel and highly effective antibacterial agent prepared through a simple process using benzimidazole and HAuCl4•3H2O. They efficiently eradicate the most challenging multidrug-resistant GNB both in vitro and in vivo while demonstrating excellent biocompatibility. This highlights their potential as a promising antibacterial agent to combat multidrug-resistant GNB.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOLOGY OF REPRODUCTION
·
2024
·
IF 3.1
Shi Heng,Zhao Xin-Hai,Peng Qin,Zhou Xian-Ling,Liu Si-Si,Sun Chuan-Chuan,Cao Qiu-Yu,Zhu Shi-Ping,Sun Sheng-Yun
Di(2-ethylhexyl) phthalate (DEHP) is a commonly used plasticizer known for its toxic effects on the male reproductive system. Green tea polyphenols (GTPs), recognized for their antioxidant and anti-inflammatory properties, have demonstrated protective effects on various organs, but the mechanisms by which GTPs mitigate DEHP-induced testicular damage remain unclear. Healthy male C57BL/6J mice were divided into five groups: control, DEHP, DEHP + GTP treatment, GTP, and oil groups. Testicular histopathological changes were assessed using hematoxylin–eosin (H&E), periodic acid-Schiff (PAS), and Masson staining. Ultrastructural alterations were examined through transmission electron microscopy. High-throughput sequencing was performed to analyze the expression of mRNA, miRNA, and lncRNA and construct an lncRNA–miRNA–mRNA regulatory network for identifying key regulatory axes. Mice in the DEHP group exhibited significant testicular damage, including reduced sperm count, mitochondrial deformation, and endoplasmic reticulum dilation. GTP treatment notably improved testicular structural integrity, restored sperm count, and alleviated mitochondrial and endoplasmic reticulum damage. Additionally, DEHP significantly increased activated CD8+ T cells, which were reduced with GTP treatment. High-throughput sequencing revealed that GTP treatment exerted protective effects through the regulation of six key lncRNA–miRNA–mRNA axes. GTPs significantly protect against DEHP-induced testicular damage, and the lncRNA–miRNA–mRNA regulatory axes play a potential role in this process.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Junshan Hu,Daobin Zhu,Keyu Guo,Bin Duan,Yuxiang Wu,Yongqiang Li,Fengyi Wang,Wei Jin,Changchun Ding
The KYb(MoO 4 ) 2 : Er 3+ , Y 3+ phosphors were synthesized by high temperature solid state reaction method. By further doping Y 3+ ions, the near-infrared (NIR, 804 nm, 4 I 9/2 → 4 I 15/2 ) luminescence of KYb(MoO 4 ) 2 : 0.1 % Er 3+ phosphor is greatly enhanced. Based on the upconversion luminescence (UCL) spectra, the optimal doping concentration of Y 3+ ions were 7.5 %. Its near-infrared UCL intensity is about 26.9 times that of undoped Y 3+ ions. Through a series of characterization methods, we find that the enhancement of NIR upconversion emission is due to the generation of defect bands. It plays a crucial role in facilitating the transfer of energy from green light level ( 2 H 11/2 , 4 S 3/2 ) to red light level ( 4 F 9/2 ) and near-infrared level. To explain the luminescence mechanism, the power dependence, UV-VI-NIR absorption spectra , excitation spectra at 804 nm and emission spectra at 380 nm were studied. In addition, the 804 nm single near-infrared UCL intensity of KYb (MoO 4 ) 2 : Er 3+ , Y 3+ shows a linear variation with temperature in the temperature range of 298 K–673 K. The maximum sensitivity is 0.13 % K −1 . This study provides a new method and theoretical support for the application of near-infrared UCL in optical temperature measurement.
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Hua-Wei Zhang,Ke Cheng,Jing-Jing Tang,Ling-Zhi Liu,Ruo-Xuan Qin,Yi-Nuo Sun,Jiang-Hua Zhang,Da-Yong Zhou
To synthesize structured phospholipids (SPLs) in an efficient and green manner is of great significance. Herein, novel Fe 3 O 4 @SiO 2 - Aspergillus oryzae lipase (AOL)/copper phosphate trihydrate nanoflowers (FLCNs) were prepared by coprecipitation method and then characterized by energy-dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer etc. FLCN activity was further evaluated by promoting functional medium-chain SPL (MCSPL) or short-chain SPL (SCSPL) synthesis via biotransesterification between soybean lecithins and medium-chain fatty acids (MCFAs) or short-chain fatty acids (SCFAs) under optimal reaction conditions. Moreover, FLCNs and free AOLs were comparatively assayed in enzymatic properties. As a result, FLCNs were successfully fabricated with sufficiently large surfaces and numerous enzyme active sites. Owing to these structural features, after reacting at 40 °C for 36 h with FLCN dosage of 6% and substrate mass ratio of 1:3:3, FLCNs exhibited much more excellent activity in MCSPL synthesis with 88.30 ± 0.63% of MCFA incorporation than benchmark enzymes and heterogeneous functional materials. Most significantly, in the first biosynthesis of functional SCSPLs facilitated by FLCNs, much superior SCFA incorporation of 66.55 ± 0.73% was obtained. Notably, FLCNs also possessed convenient magnetic separability together with superior pH and temperature tolerances, thermal and storage stabilities, and recyclability to free AOLs. In brief, the present investigation provides a new biological strategy for effective, economic, convenient, and eco-friendly SPL synthesis in food and pharmaceutical industries, highlighting its merits in functional lipid production.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 2
·1153
·
IF 4.9
Hongxue Gao,Zhiqiang Liu,Fengrui Song,Junpeng Xing,Zhong Zheng,Zong Hou,Shu Liu
Cell membrane chromatography (CMC) has been widely recognized as a highly efficient technique for in vitro screening of active compounds. Nevertheless, conventional CMC approaches suffer from a restricted repertoire of cell membrane proteins, making them susceptible to oversaturation. Moreover, the binding mechanism between silica gel and proteins primarily relies on intermolecular hydrogen bonding, which is inherently unstable and somewhat hampers the advancement of CMC. Consequently, this investigation aimed to establish a novel CMC column that could augment protein loading, enhance detection throughput, and bolster binding affinity through the introduction of covalent bonding with proteins. This study utilizes polydopamine (PDA)-coated silica gel, which is formed through the self-polymerization of dopamine (DA), as the carrier for the CMC column filler. The objective is to construct the HK-2/SiO2-PDA/CMC model to screen potential therapeutic drugs for gout. To compare the quantity and characteristics of Human Kidney-2 (HK-2) cell membrane proteins immobilized on SiO2-PDA and silica gel, the proteins were immobilized on both surfaces. The results indicate that SiO2-PDA has a notably greater affinity for membrane proteins compared to silica gel, resulting in a significant improvement in detection efficiency. Furthermore, a screening method utilizing HK-2/SiO2-PDA/CMC was utilized to identify seven potential anti-gout compounds derived fromPlantago asiaticaL. (PAL). The effectiveness of these compounds was further validated using an in vitro cell model of uric acid (UA) reabsorption. In conclusion, this study successfully developed and implemented a novel CMC filler, which has practical implications in the field.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EJNMMI Radiopharmacy and Chemistry
·
2024
·Vol. 9
·期 1
·1-17
·
IF 4.4
Chen Lijuan,Li Xiaochen,Ge Yao,Li Huiqiang,Li Ruili,Song Xiaosheng,Liang Jianfei,Zhang Weifeng,Li Xiaona,Wang Xiaoqi,Wang Yunjuan,Wu Yaping,Bai Yan,Wang Meiyun
Background A novel positron emission tomography (PET) imaging tracer, [ 18 F] SynVesT-1, targeting synaptic vesicle glycoprotein 2 (SV2A), has been developed to meet clinical demand. Utilizing the Trasis AllinOne-36 (AIO) module, we’ve automated synthesis to Good Manufacturing Practice (GMP) standards, ensuring sterile, pyrogen-free production. The fully GMP-compliant robust synthesis of [ 18 F] SynVesT-1 boosting reliability and introducing a significant degree of simplicity and its comprehensive validation for routine human use. Results [ 18 F] SynVesT-1 was synthesized by small modifications to the original [ 18 F] SynVesT-1 synthesis protocol to better fit AIO module using an in-house designed cassette and sequence. With a relatively small precursor load of 5 mg, [ 18 F] SynVesT-1 was obtained with consistently high radiochemical yields (RCY) of 20.6 ± 1.2% (the decay-corrected RCY, n = 3) at end of synthesis. Each of the final formulated batches demonstrated radiochemical purity (RCP) and enantiomeric purity surpassing 99%. The entire synthesis process was completed within a timeframe of 80 min (75 ± 3.1 min, n = 3), saves 11 min compared to reported GMP automated synthesis procedures. The in-human PET imaging of total body PET/CT and time-of-flight (TOF) PET/MR showed that [ 18 F] SynVesT-1 is an excellent tracer for SV2A. It is advantageous for decentralized promotion and application in multi-center studies. Conclusion The use of AIO synthesizer maintains high production yields and increases reliability, reduces production time and allows rapid training of production staff. Besides, the as-prepared [ 18 F] SynVesT-1 displays excellent in vivo binding properties in humans and holds great potential for the imaging and quantification of synaptic density in vivo.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Susu Zhao,Qian Dang,Aiqing Cao,Marshet Getaye Sendeku,Hai Liu,Jian Peng,Yameng Fan,Hui Li,Fengmei Wang,Yun Kuang,Xiaoming Sun
Ruthenium (Ru)-based catalysts have demonstrated promising utilization potentiality to replace the much expensive iridium (Ir)-based ones for proton exchange membrane water electrolysis (PEMWE) due to their high electrochemical activity and low cost. However, the susceptibility of RuO2-based materials to easily be oxidized to high-valent and soluble Ru species during the oxygen evolution reaction (OER) in acid media hinders the practical application, especially under current density above 500 mA cm–2. Here, a manganese-doped RuO2 catalyst with the hydroxylated metal sites (i.e., H–Mn0.1Ru0.9O2) is synthesized for acidic OER assisted by hydrogen peroxide, where the hydroxylation results in the valence state of the Ru sites below +4. The H–Mn0.1Ru0.9O2 catalyst demonstrates an overpotential of 169 mV at 10 mA cm–2 and promising stability for an OER over 1000 h in an acidic electrolyte. A PEMWE device fabricated with the H–Mn0.1Ru0.9O2 catalyst as the anode shows a current density of 1 A cm–2 at ∼1.65 V, along with a low degradation over continuous tens of hours. Differential electrochemical mass spectrometry (DEMS) results and theoretical calculations confirm that H–Mn0.1Ru0.9O2 performs the OER through the adsorbate evolution mechanism (AEM) pathway, where the synergistic effect of hydroxylation and Mn doping in RuO2 can effectively enhance the stability of Ru sites and lattice oxygen atoms.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Tianren Wang,Jingru Gao,Jinghan Xu,Yuxiang Hong,Ronghuan Du,Xian Zheng,Peng Wang
Cystatins are well known as a vast superfamily of functional proteins participated in the reversible competitive inhibition of cysteine proteases. Currently, increasing evidences point to the extensive phylogenetic diversity and crucial immune roles of type-2 cystatins in the vertebrate species. However, no information is available regarding the homologue in cephalochordate amphioxus, the representative of most basal living chordates, whose immune regulation are still ambiguous. Here, we clearly identified the presence of type-2 cystatin gene in amphioxus Branchiostoma japonicum , termed Bjcystatin-2, which was structurally characterized by typical wedge-shaped cystatin feature. Evolutionary analyses revealed that Bjcystatin-2 is the putative ancestral type-2 cystatin for chordates, with gene diversity emerging through duplication events. The expression of Bjcystatin-2 showed tissue-specific profile and was inducible upon invasive pathogens. Significantly, the recombinant Bjcystatin-2 exhibited not merely cathepsin L inhibitory activity, but also the ability to bind with bacteria and their characteristic molecules. Furthermore, Bjcystatin-2 also showed the capacity to enhance the macrophage-driven bacterial phagocytosis and to attenuate the generation of pro-inflammatory cytokines within macrophages. In summary, these findings demonstrate that Bjcystatin-2 exhibits dual role acting as both a protease inhibitor and an immunoactive molecule, greatly enriching our understanding of immune defense mechanisms of type-2 cystatin within the amphioxus.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY
·
2025
·
IF 4.7
Jinchang Zhang,Xuefeng Cheng,Jicai Jiang,Apiladda Pattanateeradetch,Shuai Shi,Chanat Chokejaroenrat,Qingfeng Xu,Jianmei Lu
Selective enrichment of cesium ions (Cs+) at ultralow concentrations is essential for resource recovery and radioactive waste disposal, yet efficient adsorbents are lacking. Herein, we reported a Prussian blue analogue (K2Cu3(Fe(CN)6)2, Cu-PBA) decorated on MXene nanosheets by in situ fabrication, forming a composite material termed PMX, for enhanced adsorption of Cs+ in acidic solutions and seawater. The stable, negatively charged MXene effectively anchors Cu2+ precursors and promotes Cs+ adsorption. The synergistic interaction between MXene and the in situ-synthesized Cu-PBA significantly enhances the adsorption performance and water stability of PMX in both acidic solutions and seawater. PMX achieves rapid adsorption equilibrium within 5 min, with a high adsorption capacity of 408.2 mg/g at pH 1, surpassing conventional adsorbents. Moreover, PMX shows excellent Cs+ selectivity (Kd = 68,361.7 mL/g), cycle stability, and notable anti-irradiation ability, demonstrating superior efficiency in Cs+ enrichment from complex matrices. The adsorption mechanism involves electrostatic attraction and K+/Cs+ ion exchange, facilitated by MXene’s functional groups and the Cu-PBA structure. These findings underscore the excellent potential of PMX as an efficient adsorbent for resource enrichment and the removal of radioactive elements such as Cs+.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2025
·
IF 2.2
Xianming Zhang,Yanping Li,Yongli Wu,Yunfei Wang,Panpan Yan,Zhilei Zheng,Hongyu Peng,Yuexin Chu
Liquid–liquid equilibrium (LLE) for the binary systems of 1-octene +1-ethyl-3-methylimidazolium acetate ([EMIM][Ac]), 1-octene +1-butyl-3-methylimidazolium acetate ([BMIM][Ac]), 1-octene +1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF 4 ]), 2-hexanone + [EMIM][Ac], and 2-hexanone + [BMIM][Ac], and the ternary systems of 1-octene +2-hexanone + [EMIM][Ac], 1-octene +2-hexanone + [BMIM][Ac], and 1-octene +2-hexanone + [BMIM][BF 4 ] were measured at 298.15 K, 303.15 K and 313.15 K under 101.3 kPa. The solute distribution coefficient ( D ) and selectivity ( S ) were calculated to investigate the efficiencies of [EMIM][Ac], [BMIM][Ac], and [BMIM][BF 4 ] as solvents. Moreover, the temperature dependencies of D and S were investigated in this study. NRTL and UNIQUAC models were applied to correlate the experimental LLE data.
✓ 已验证
产品关联
DOI
高影响因子
FUEL
·
2024
·
IF 6.7
Yangshuo Liu,Keke Huang,Yao Meng,Chubo Wang,Liang Qiao,Wei Cai,Yaotian Yan,Xiaohang Zheng
Searching for highly efficient electrocatalysts for the hydrogen evolution reaction (HER) is principal to the development electrolytic water production industry. Experimental screening of highly active electrocatalysts is time-consuming and complicated. In this work, an Artificial Neural Network model is proposed to accelerate the screening for Ag(M) catalysts (M = Al, Si, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, Ge, Mo, Ru, Rh, Pd, In, Sn, Sb, W, Re, Os, Ir, Pt, Au and Hg), which is used to predict the Gibbs free energy of hydrogen. The Ag(Ni) catalyst is identified as a potential electrocatalyst with the nearly ideal (ΔG H ), which affords the relatively low overpotentials of 159 mV for HER at 10 mA cm −2 . According to the prediction of our ANN model, we synthesized Ag(Mn), Ag(Co), and Ag(Cu) catalysts. The Ag(Ni) catalyst exhibits the best HER activity, which is 120 mV smaller than Ag(Mn), Ag(Co), and Ag(Cu) catalysts. The incorporation of Ni effectively optimizes the electronic environment of the materials, which drives the upshift of d-band center and drastically reduces the Gibbs free energy of hydrogen (ΔG H ). Our method is significantly more efficient, running 1184 times faster than the traditional DFT method. Our work paves an efficient way to design and develop potential HER catalysts.
✓ 已验证
产品关联
DOI
高影响因子
BMC MUSCULOSKELETAL DISORDERS
·
2024
·Vol. 25
·期 1
·1-13
·
IF 2.2
Xu Kuishuai,Zhang Liang,Wang Tianrui,Yu Tengbo,Zhao Xia,Yu Ning,Zhang Yingze
Objective To assess the mechanism of supraspinatus tendinopathy induced by type 2 diabetes mellitus (T2DM) in rats using untargeted metabolomics analysis. Methods The liquid chromatography-mass spectrometry (LC-MS)-based untargeted metabolomics approach was used to screen tendon biomarkers of supraspinatus tendinopathy in rats with T2DM. Seventy-eight Sprague-Dawley rats were divided into normal group (NG) and T2DM groups. Rats in T2DM groups were divided into 12-week (T2DM-12w), and 24-week (T2DM-24w) subgroups according to the time point of the establishment of the T2DM rat model. Histological evaluation (modified Bonar score) and biomechanical testing were used to analyze the adverse effects of type 2 diabetes on the tendon of the supraspinatus muscle in rats.Three comparable groups were set up, including T2DM-12w group vs. NG, T2DM-24w group vs. NG, and T2DM-24w group vs. T2DM-12w group. Differentially expressed metabolites (DEMs) in the supraspinatus tendons in the three groups of rats were analyzed using LC-MS, and data were analyzed using multivariate statistical methods to screen potential biomarkers. The DEMs included in the intersection of the three groups were identified as those associated with the development of diabetic supraspinatus tendinopathy, and trend analysis and pathway topology analysis were performed. Results With the progression of diabetes, the tendinopathy of the supracinatus muscle of diabetic rats gradually intensified, mainly manifested as inflammatory reactions, disordered collagen fibers, fat infiltration, and increased modified Bonar score. The intersection of DEMs among the three comparable groups was resulted in the identification of 10 key DEMs, in which melezitose and raffinose showed a continuous increasing trend with the prolongation of disease course. By pathway topology analysis, 10 DEMs ( P < 0.01) were mainly associated with the pathways of galactose metabolism, which could be involved in the development of diabetes-induced supraspinatus tendinopathy. Conclusion T2DM causes tendinopathy of the supraspinatus muscle in rats. 10 key DEMs obtained by untargeted metabolomics assay suggested that the development of diabetes-induced supraspinatus tendinopathy was associated with changes in metabolic pathways, such as galactose metabolism. melezitose and raffinose hold promise as a biomarker for disease discrimination and/or disease indication in diabetic supraspinatus tendinopathy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）