CHEMICAL ENGINEERING JOURNAL
·
2014
·
IF 4.058
Zhi-yun Kong,Jun-fu Wei,Yong-hua Li,Na-na Liu,Huan Zhang,Yue Zhang,Li Cui
The quaternary ammonium fibers which could remove Cr(VI) ions rapidly were prepared by radiation-induced grafting of 2-(dimethylamino)ethyl methacrylate (DMAEMA) onto polypropylene (PP) fibers (PP-g-DMAEMA) and modifying with 1-bromoalkanes. The FTIR, FESEM, XPS and TG-DTG spectra manifested that the quaternary ammonium group was introduced onto the surface of PP fibers. The main factors influencing the adsorption of Cr(VI) ions including contact time, pH of the aqueous solution and adsorption dosage were investigated. The removal rate could reach 97% within 4 min and the kinetics study indicated that adsorption process was surface reaction controlled. Maximum adsorption capacities of Cr(VI) ions at 25 °C were 105.8 mg/g based on the Langmuir isotherms. These results suggested that the quaternary ammonium fibers have potential application for the removal of hazardous metal pollutants from wastewater highly and rapidly.
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International Journal of Nanomedicine
·
2022
·
IF 7.033
Wei Shang,Xin Zhao,Fan Yang,Dongyi Wang,Le Lu,Zihan Xu,Zhiming Zhao,Hui Cai,Junyi Shen
Background: Compared with traditional drugs, nanomaterial drugs have the benefits of improving the solubility, bioavailability, and absorption rate of insoluble drugs. Nanoporous complexes can increase the efficiency with which drugs can penetrate the blood-brain barrier and reach target organs. Ginsenoside Rg1 is an effective drug that promotes angiogenesis. Ginsenoside Rg1 composite nanoparticles were employed to induce the expression of several key epigenetic enzymes and then activate the VEGF and Notch pathways after the onset of ischemic brain lesions. Methods: We constructed nanoparticles to fully encapsulate the therapeutic drug (ginsenoside Rg1), which can be transferred into brain tissue via the receptor-mediated transfer of drug-encapsulated nanoparticles. Evaluation of the therapeutic effect of ginsenoside Rg1 complex nanovesicles (CNV) was performed by in vitro and in vivo experiments. Real-time polymerase chain reaction (RT- PCR), Western blot, immunohistochemistry staining (IHC), and Co-immunoprecipitation (co-IP) were employed to screen for epigenetic enzymes with an up-regulated expression post ginsenoside Rg1-CNV intervention. RNA sequencing, shRNA knockdown, and chromatin Immunoprecipitation (ChIP) sequencing were performed to detect the target genes of ginsenoside Rg1-CNV that regulate angiogenesis. Then, bioinformatic analysis was performed to investigate the mechanism of action of epigenetic modifying enzymes in regulating target genes. Results: The average of the synthesized ginsenoside Rg1-CNV was 203.78±6.83 nm, the polydispersion index was 0.135±0.007, and the Zeta potential was 23.13±1.65 mV. Through in vivo and in vitro experiments, we found that it promotes the proliferation, migration, and tubular formation of brain microvascular endothelial cells (BMECs). Meanwhile, the intervention of ginsenoside Rg1-CNV promoted the demethylation of H3K27me3 within the promoter region of VEGF-A and Jagged1 genes and reduced the H3K27me3 modification within this region. Conclusion: The ginsenoside Rg1 nanoparticles may be an available blood-brain barrier penetrating agent for ischemic stroke.
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ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
·
2014
·
IF 1.862
Demei Xu,Lihua Hu,Xiaomin Xia,Jianbo Song,Lingrui Li,Erqun Song,Yang Song
Tetrachlorobenzoquinone (TCBQ) is an active metabolite of pentachlorophenol (PCP). Although PCP has been investigated extensively, there are only a few reports describing the toxicity effect of TCBQ, and no report regarding TCBQ-induced liver injury in vivo . In the current study, we aimed to examine the acute hepatic toxicity of TCBQ in the mice model. Chlorogenic acid (CGA) exhibits promising antioxidant activity in the past studies, thus, the second aim of this study was to evaluate the protective effect of CGA on TCBQ-induced liver injury. Our results indicated TCBQ-intoxication caused marked liver cell necrosis and inflammation but not apoptosis, and this damage was alleviated by CGA treatment. Meantime, TCBQ-intoxication enhanced serum ALT, AST activities, TBIL content, hepatic oxidative stress and lipid peroxidation, decreased GSH content and inhibited the activities of antioxidant enzymes. Western blot and immunohistochemical analysis showed that TCBQ marked up-regulated HO-1 and NQO1 expression. On the other hand, pretreatment of CGA reduced TCBQ-induced liver damage remarkably. Taking together, these results revealed that TCBQ has strong hepatic toxic effect, and at least a part of this effect is initiated by free radical and relieved with CGA administration.
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高影响因子
Polymers
·
2022
·Vol. 14
·期 14
·2872
·
IF 4.967
Zhengshi Li,Huimin Lin,Shanwei Shi,Kai Su,Guangsen Zheng,Siyong Gao,Xuan Zeng,Honglong Ning,Meng Yu,Xiang Li,Guiqing Liao
Bone healing is a complex process that requires the participation of cells and bioactive factors. Stromal derived factor-1 α (SDF-1α) and magnesium ions (Mg2+) both are significant bioactive factors for cell recruitment and osteogenesis during bone regeneration. Thus, a bifunctional hydrogel containing a sequential delivery system is fabricated to improve osteogenesis. During sequential delivery of the hydrogel, SDF-1α is predominantly released at the early stage of bone mesenchymal stem cells (BMSCs) recruitment, while Mg2+are constantly delivered at a later stage to improve osteogenic differentiation of recruited cells. In addition, due to the early release of SDF-1α, the hydrogel showed strong BMSCs recruitment and proliferation activity. Mg2+can not only induce up-regulation of osteogenic gene expression in vitro, but also promote bone tissue and angiogenesis in vivo. Taken together, the injection of xanthan gum-polydopamine crosslinked hydrogel co-loading SDF-1α and Mg2+(XPMS hydrogel) provides a novel strategy to repair bone defects.Keywords:hydrogel;BMSCs recruitment;sequential drug release;osteogenic differentiation;bone regeneration
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JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2022
·
IF 6.437
Ping Ye,Heng Zhai,Yueqi Li,Wenkun Zhu,Guangcheng Yang,Changping Guo
Breaking the inherent contradiction between high energy and insensitivity and pursuing enviro-friendly, high safety and excellent thermal decomposition properties are urgently desired for the development of modern composite solid propellants . 2,6-Diamino-3,5-dinitropyrazine-1-oxide (LLM-105) nanoparticles were uniformly deposited on the surface of ammonium perchlorate (AP) by ultrasonic shock with Estane as modifier. In the current work, it was concluded that the crystal phase and morphology of AP remained unchanged, and that complete and dense AP@LLM-105 core-shell composites were obtained at 15% LLM-105. In addition, the high temperature decomposition peak of AP@LLM-105 is advanced to 296.5 ℃ and the heat release is increased to 1271 J/g. More importantly, AP@LLM-105 also showed excellent performance in sensitivity test, with impact sensitivity H 50 and friction sensitivity as low as 39 cm and 18%. Obviously, low-pollution AP@LLM-105 with high safety performance and excellent thermal decomposition performance provides a good candidate for composite solid propellants.
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JOURNAL OF COORDINATION CHEMISTRY
·
2017
·
IF 1.795
Shan-Shan Zhao,Peng Wang,Li-Na Wang,Lin Fu,Xiao-Meng Han,Jie Qin,Shao-Song Qian
The multidentate Schiff-base ligand N′-(1-(pyrazin-2-yl)ethylidene)isonicotinohydrazide (HL) has been prepared. Reaction with zinc, copper, and silver nitrate afford three complexes, [Zn(HL′)2](NO3)2·3H2O (1), {[Cu2(L)2(NO3)(H2O)2]·NO3}n (2) and {[Ag2(L)2]·3H2O}n (3). These complexes have been characterized by elemental analysis, IR spectroscopy, and single-crystal X-ray diffraction. In 1, HL is a neutral tridentate ligand, whereas in 2 and 3, HL is a deprotonated tetradentate ligand. The hydrogen bonding interactions between NO3− and the host framework result in various supramolecular polymeric structures: a 2-D layer for 1 and 3-D network for 2 and 3. The antibacterial activities of these complexes have been investigated and the results indicate that 3 showed good antibacterial activities.
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BIOORGANIC CHEMISTRY
·
2018
·
IF 3.929
Jianpeng Su,Jiayun Liu,Cheng Chen,Yuejuan Zhang,Kewu Yang
The superbug infection caused by New Delhi metallo-β-lactamase (NDM-1) has grown into an emerging threat, labelling and inhibition of NDM-1 has proven challenging due to its shuttling between pathogenic bacteria. Here, we report a potent covalent scaffold, ebsulfur, for targeting the protein in vitro and in vivo . Enzymatic kinetic study indicated that eighteen ebsulfurs gained except 1a – b and 1f inhibited NDM-1, exhibiting an IC 50 value ranging of 0.16–9 μM, and 1g was found to be the best, dose- and time-dependent inhibitor with an IC 50 of 0.16 μM. Also, these ebsulfurs effectively restored the antibacterial activity of cefazolin against E. coli expressing NDM-1, and the best effect was observed to be from 1g , 1i and 1n , resulting in an 256-fold reduction in MIC of the antibiotic at a dose of 16 μg/mL. The equilibrium dialysis study implied that the ebsulfur disrupted the coordination of one Zn(II) ion at active site of NDM-1. Labelling of NDM-1 using a constructed fluorescent ebsulfur Ebs - R suggested that the inhibitor covalently bound to the target through SDS-PAGE analysis in vitro . Also, labelling NDM-1 in living E. coli cells with Ebs - R by confocal microscopic imaging showed the real-time distribution change process of intracellular recombinant protein NDM-1. Moreover, the cytotoxicity of these ebsulfurs against L929 mouse fibroblastic cells was tested, and their capability to restore antibacterial activity of antibiotic against clinical strains E. coli EC08 producing NDM-1 was determined. The ebsulfur scaffold proposed here is valuable for development of the covalent irreversible inhibitors of NDM-1, and also for labelling the target in vitro and in vivo .
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JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL
·
2015
·
IF 3.615
Yong-Qi Li,Peng Wang,Heng Zhang,Xiao-Li Zhao,Yong Lu,Zora Popović,Ye Liu
The neutral diphosphine of 1 and the corresponding ionic diphosphine of 2 after quaternization 1 by MeOTf were prepared and investigated as the wide bite angle and bulky steric ligands to control the regioselectivity in Rh(acac)(CO) 2 -catalyzed hydroformylation of 1-octene. As for 2 , due to the electrostatic repulsive force of the two positive charges, the involved two-PPh 2 were located in trans -position with the relatively longer P 1… P 2 distance [8.0231(11) Å] to prefer to aa-site coordination in trigonal bipyramid H(alkene)Rh(CO)(P–P) intermediate, which renders less bulky steric hindrance for the regioselectivity to linear aldehyde as a result of low L / B ratio of 1.9. Due to the strong electron-withdrawing effect from the positive-charged imidazolium rings on the neighbored P-atoms, 2 with strong π-acceptor ability can develop π-backdonation interaction between Rh and P linkage to favor the dissociation of CO ligand and facilitate the selectivity to nonanals. In contrast, 1 with no electrostatic repulsive effect and less steric hindrance, the two-PPh 2 were able to be conveniently located in cis -position with the relatively shorter P 1… P 2 distance [ P 1… P 2, 6.2096(10) Å], which corresponded to the preferential linear regioselectivity with L / B = 7.1. The in situ high pressure spectroscopy analysis indicated that the involvement of 1 in Rh(acac)(CO) 2 -catalyzed hydroformylation of 1-octene could greatly facilitate the formation and stability of the active Rh–H species ( ν 2047 cm −1 ).
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SOLAR ENERGY
·
2018
·
IF 4.374
Xichuan Yang,Weihan Wang,Yanyun Zhang,Licheng Sun
P-type doping is a rational strategy for the enhancement of hole transporting properties of the organic semiconductors as well as the device performance of organic photo-electric devices. We originally introduce a stable and solution processed p-type dopant based on N-oxoammonium salts termed 2,2,6,6-tetramethyl-1-oxopiperidinebromide salt (TEMPO-Br) for 2,2′,7,7′-tetrakis(N, N-di-p-methoxyphenyl-amine)9,9′-spirobifluorene (Spiro-OMeTAD) based solid state dye sensitized solar cells (ssDSC). By introducing TEMPO-Br doped Spiro-OMeTAD and a commercialized D-π-A sensitizer into ssDSCs, a promising device performance of 6.83% is achieved under simulated AM 1.5G solar irradiation (100 mW cm −2 ), which is significantly better than the control devices (∼1.7 times). The doping effect of TEMPO-Br on the photophysical and electrochemical properties of Spiro-OMeTAD, solid state device performance, cationic dye regeneration kinetics and preliminary long-term stability have been systematically investigated. This work indicates a potential application of N-oxoammonium salts as chemical P-type dopants for triphenylamine based hole transporting materials in solid state photo-electric devices.
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ORGANIC PROCESS RESEARCH & DEVELOPMENT
·
2022
·
IF 3.858
Ling He,Wenjie Ye,Youyu Xie,Qinghai Liu,Hualei Wang,Dongzhi Wei
Bioreductions catalyzed by ketoreductases play an important role in the synthesis of chiral alcohols. However, the synthesis of (R)-2-chloro-1-(3,4-difluorophenyl)ethanol (24b), an important chiral intermediate for the synthesis of the anticoagulant ticagrelor, poses significant challenges in terms of high substrate concentration requirements that limit its production. In this study, a novel NADH-dependent, short-chain dehydrogenase, PpKR8, from Paraburkholderia phymatum STM815, exhibited excellent enantioselectivity and high activity for the production of (R)-CFPL (24b) through the reduction of 2-chloro-1-(3,4-difluorophenyl)ethanone (24a). The coexpression of PpKR8 and glucose dehydrogenase from Bacillus subtilis in Escherichia coli allowed up to 300 g/L (1.57 M) CFPO (24a) to be completely converted into 24b with 99.9% enantiomeric excess and a high space–time yield (728 g/(L day)). Substrate specificity assays demonstrated a broad substrate spectrum for PpKR8, which included 35 α/β-ketoesters, aromatic ketones, and heterocyclic ketones. Moreover, three additional optically pure chiral alcohols that are used as important drug intermediates were synthesized at high substrate concentrations (150–330 g/L), demonstrating the excellent industrial potential of PpKR8-mediated bioreductions.
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JOURNAL OF STRUCTURAL CHEMISTRY
·
2022
·Vol. 63
·期 10
·1610-1615
·
IF 1.004
Wang G. -F.,Sun S. -W.
Abstract Two coordination polymers, {[Co 2 (L 1 ) 2 (adc) 2 ]·5H 2 O} n ( 1 ) and {[Ni(L 1 ) 2 (Hadc) 2 ]} n ( 2 ) (L 1 = bis(4-(1 H -imidazol-1-yl)phenyl)methanone, H 2 adc = adamantane-1,3-dicarboxylic acid), have been synthesized and structurally characterized. In 1 , Co1 is five-coordinated by three oxygen atoms of two adc 2– and two nitrogen atoms from two L 1 ligands. The neighboring [Co(adc)] 2 dimers are linked through four L 1 ligands to form a 1D ladder structure. In 2 , Ni1 is six-coordinated by two oxygen atoms from two Hadc – ligands, four nitrogen atoms from four distinct L 1 ligands to give a slight distorted octahedral geometry. The [Ni(Hadc) 2 ] units are bridged by L 1 ligands to form 1D looped chains.
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JOURNAL OF VIROLOGY
·
2018
·Vol. 92
·期 17
·
IF 4.368
Cong-Cong Wu,Xuan Jiang,Xian-Zhang Wang,Xi-Juan Liu,Xiao-Jun Li,Bo Yang,Han-Qing Ye,Thomas Harwardt,Man Jiang,Hui-Min Xia,Wei Wang,William J. Britt,Christina Paulus,Michael Nevels,Min-Hua Luo
The mechanisms underlying neurodevelopmental damage caused by virus infections remain poorly defined. Congenital human cytomegalovirus (HCMV) infection is the leading cause of fetal brain development disorders. Previous work has linked HCMV infection to perturbations of neural cell fate, including premature differentiation of neural progenitor cells (NPCs). Here, we show that HCMV infection of NPCs results in loss of the SOX2 protein, a key pluripotency-associated transcription factor. SOX2 depletion maps to the HCMV major immediate early (IE) transcription unit and is individually mediated by the IE1 and IE2 proteins. IE1 causes SOX2 downregulation by promoting the nuclear accumulation and inhibiting the phosphorylation of STAT3, a transcriptional activator of SOX2 expression. Deranged signaling resulting in depletion of a critical stem cell protein is an unanticipated mechanism by which the viral major IE proteins may contribute to brain development disorders caused by congenital HCMV infection.IMPORTANCE Human cytomegalovirus (HCMV) infections are a leading cause of brain damage, hearing loss, and other neurological disabilities in children. We report that the HCMV proteins known as IE1 and IE2 target expression of human SOX2, a central pluripotency-associated transcription factor that governs neural progenitor cell (NPC) fate and is required for normal brain development. Both during HCMV infection and when expressed alone, IE1 causes the loss of SOX2 from NPCs. IE1 mediates SOX2 depletion by targeting STAT3, a critical upstream regulator of SOX2 expression. Our findings reveal an unanticipated mechanism by which a common virus may cause damage to the developing nervous system and suggest novel targets for medical intervention.
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