Food & Function
·
2021
·Vol. 12
·期 14
·6490-6501
·
IF 5.396
Ao Sun,Xiaoqin Hu,Huijian Chen,Yulan Ma,Xiyue Yan,Dangsheng Peng,Jie Ping,Youe Yan
Ursolic acid (UA) shows an effect on obesity and related metabolic diseases, but its mechanism of action remains unclear. We found that UA clearly reduced the body weight and adipose tissue mass and improved the glucose tolerance and insulin sensitivity in obese male mice. UA treatment significantly reduced the volume and weights of the epididymal white adipose tissue (eWAT) and inguinal subcutaneous white adipose tissue (igSWAT) of HFD-fed mice, respectively. UA also decreased the expression of genes involved in adipocyte differentiation and lipogenesis in igSWAT. Real-time PCR and immunohistochemistry showed that the expression of beiging-related genes 4-1BB factor (CD137), T-box transcription factor 1 (TBX1), and transmembrane protein 26 (TMEM26) were significantly increased in the UA treatment group. UA treatment significantly reduced the weight of gastrocnemius muscle (GM) and lipid droplets in the GM. UA treatment significantly upregulated the expression of PR domain-containing 16 (PRDM16), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α), and fibronectin type 3 domain-containing protein 5 (FNDC5) in GM and igSWAT. UA also stimulated irisin secretion in the serum. In conclusion, these results indicate that UA plays an anti-obesogenic role by increasing the secretion of irisin and promoting the beiging of WAT.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2021
·Vol. 33
·期 10
·2252-2259
·
IF 3.223
Jilong Li,Yanhui Wang,Rushuo Li,Bowen Lu,Yungang Yuan,Hongwei Gao,Shiwei Song,Shuyu Zhou,Jianbing Zang
A self-modified film electrode consisting of homogeneous snowflake-shaped nanoparticles on the amorphous carbon substrate (HNAC) was prepared by low temperature carbonization of phenolic resin. Such a unique structure was beneficial to enhance the electroanalysis signal responds. Simultaneous detection of DA and UA was performed on the HNAC using differential pulse voltammetry (DPV) at pH 8 phosphate buffer. The well-defined oxidation peak potential separation reached 260 mV between DA and UA. Meanwhile, the detection limit of HNAC were 0.401 μM (DA) and 2.800 μM (UA).
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Yiyang Sun,Zhaorui Song,Xu Ni,Pierre Dramou,Hua He
In this study, a new ratio fluorescent gel probe based on boronic acid-modified BA-Tb-MOG was constructed and successfully applied to the analysis and detection of dopamine (DA). The dual emission BA-Tb-MOG probe sensing is realized by adjusting the energy conversion efficiency of the boronic acid-based ligand and Tb 3+ ions. The 5-boronobezene-1, 3-dicarboxylic acid (DBA) organic ligand not only acted as the “antenna” to sensitize the luminescence of Tb 3+ but also provided recognition sites for DA molecules. Dual fluorescence emission is generated at 325 nm and 545 nm under the excitation of 295 nm. The nucleophilic covalent binding of the boronic acid group to the cis -diol structure molecule provides a good basis for the specific and highly sensitive detection of DA. Owing to the porous characteristics and the surface effect of the MOF, as well as the unique nucleophilic reaction between the boronic acid group and DA, the novel gel nanoprobe exhibited outstanding characteristics for detection of DA, such as such as simple preparation, fluorescence stabilization, high sensitivity, and selectivity. It has been successfully applied to the detection of DA in human serum samples. This ratio fluorescence detection strategy provides an alternative sensing method for the rapid and sensitive analysis and determination of dopamine in complex biological samples.
✓ 已验证
产品关联
DOI
高影响因子
ELECTROANALYSIS
·
2021
·Vol. 33
·期 10
·2243-2251
·
IF 3.223
Kunpeng Miao,Long Yan,Ran Bi,Xiaoyan Ma
RGO/Au/Ni electrode was manufactured by a convenient, controllable, and environmental process, which was carried out by cyclic voltammetry (CV), and in this process, graphene-gold nanohybrid materials were simultaneously deposited on the nickel foam. Then the GOx was immobilized on the RGO/Au/Ni electrode by covalent bonding, and obtained the enzymatic biosensor. Scanning electron microscope (SEM) and Raman spectroscopy were adopted to confirm the microstructure of the fabricated RGO/Au/Ni electrode. Fourier transform infrared spectroscopy (FT-IR) was used to characterize the prepared enzymatic electrode. CV, chronoamperometry, and electrochemical impedance spectroscopy (EIS) were used to characterize the electrochemical performance of the fabricated enzymatic biosensor. It is found that AuNPs were well dispersed on the wrinkled RGO sheets, and the biosensor had a high sensitivity to glucose (32.83 μA ⋅ mM −1 ⋅ cm −2 ) with a wide linear range (0.15 26.15 mM), the strengths of anti-interference ability, good stability, and repeatability, etc.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2021
·
IF 4.464
Rui Feng,Yao Chu,Xiaohao Wang,Qinfeng Wu,Fei Tang
Flexible biosensors have the great potential to be applied in medical diagnosis, wearable devices, fermentation and other fields. This paper proposes a manufacturing scheme of a flexible enzymatic glucose sensor coated with polyurethane modified by poly(ethylene glycol), PU-PEG, as a protective membrane, which can overcome the problems of poor consistency and short service life of the electrochemical sensor. A platinum-black electrode formed by electroplating platinum-black nanoparticles onto a flexible gold electrode is used as an enzyme carrier. The enzyme is electrosprayed evenly onto the electrode to avoid aggregation and to control quality. Glutaraldehyde is used as a cross-linking agent to immobilise the enzyme layer. Additionally, using PU-PEG as the protective membrane improves the anti-interference performance, controls the enzymatic reaction rate and makes long-term stable operation of the sensor possible. The experimental results show that the linear range of the sensor is 0.5 ~ 25 mM, the minimum detection limit is 0.25 mM, and the sensitivity is as high as 3.418 μA/mM. The measurement results of the sensors show good consistency, with a maximum absolute deviation of only 5.52%. The effective working time in 37℃ bovine serum with a glucose concentration of 5 mM is up to 23 days.
✓ 已验证
产品关联
DOI
高影响因子
ELECTROANALYSIS
·
2021
·Vol. 34
·期 2
·369-374
·
IF 3.223
Xiao Liu,Mei-Hao Xiang,Xinyue Zhang,Qin Li,Xiaoya Liu,Wenjing Zhang,Xia Qin,Fengli Qu
In this work, we reported the development of a nickel metal-organic framework nanosheet array on Ti-mesh (Ni-MOF/TM) as an enzyme-free electrochemical sensing platform for H 2 O 2 determination. The as-obtained sensor exhibited outstanding detection properties of H 2 O 2 , which might be gifted from the large specific surface area, abundant active sites of Ni-MOF nanoarrays. The sensor displayed a good linear range (0.8 μM–4.6×10 3 μM), a detection limit as low as 0.26 μM, a high sensitivity (307.5 μA mM −1 cm −2 ), and a rapid response. Moreover, this enzyme-free sensor is promising for point-of-care (POC) testing of H 2 O 2 in human serum attribute to the excellent performance of Ni-MOF and the simple preparation process of the sensor.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 56
·期 1
·442-456
·
IF 3.553
Ma Xiaoqing,Tang Kang-lai,Yang Mingyu,Shi Wenbing,Zhao Wenxi
Reliable determination of hydrogen peroxide (H 2 O 2 ) and glucose is vital in various fields, especially implicated in diagnosis of diabetes and oxidative stress-related diseases. Herein, yolk–shell hollow Ni/NiO@C microspheres were template-synthesized with well-defined Ni-MOF (metal–organic framework) and further worked as a bifunctional electrocatalyst for glucose oxidation in alkaline solution and H 2 O 2 reduction in neutral solution, of which the unique structure and rich pore openings provide large accessible surface areas, while the uniformly distributed Ni, NiO and C efficiently catalyze and facilitate the reaction of glucose and H 2 O 2 , thus achieving a wide linear range of 0.01 to 10 mM with a sensitivity of 1291 μA mM −1 cm −2 and a detection limit of 0.116 μM for glucose and offering a sensitivity of 32.09 μA mM −1 cm −2 , a detection limit of 0.9 μM and a linear range up to 80.7 mM for H 2 O 2 , as well as good selectivity, excellent reproducibility and long-term stability for two analytes. What’s more, the as-constructed sensors realize quantitative detection glucose in blood serum with satisfying results and can use to monitor H 2 O 2 in neutral situation. This work opens an avenue to design high-performance sensoring materials but also becomes a great candidate in practical application.
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 47
·20797-20805
·
IF 3.288
Hongmi Liu,Rujie Xiong,Peng Zhong,Guangli Li,Jun Liu,Jingtao Wu,Ying Liu,Quanguo He
In this study, an ultrasensitive voltammetric sensor was constructed for the simultaneous detection of dopamine (DA) and uric acid (UA) using shuttle-like α-Fe2O3 nanoparticle/nitrogen-doped graphene nanocomposites (Fe2O3–NG). The surface morphologies, crystalline structures, and electrochemical properties of Fe2O3–NG were investigated by scanning electron microscopy, powder X-ray diffraction, cyclic voltammetry, and AC impedance spectroscopy. Coupling shuttle-like α-Fe2O3 with NG nanosheets not only enlarges the electrochemically active surface area and prevents the agglomeration of α-Fe2O3 NPs or NG nanosheets, but also increases the electroconductivity of α-Fe2O3 NPs and boosts its catalytic ability, thereby affording more electroactive sites for the adsorption and oxidation of target analytes. As expected, the Fe2O3–NG/GCE shows extraordinary electrocatalytic oxidation capacities toward DA and UA over two wide dynamical response concentration ranges of 0.5–10 μM and 10–100 μM, with low detection limits (LOD) of 0.080 and 0.075 μM for DA and UA, respectively. Moreover, the Fe2O3–NG/GCE shows high anti-interference ability against several potential interferences and retains stable voltammetric responses for at least a week. Finally, the Fe2O3–NG/GCE achieves the simultaneous determination of DA and UA in human serum with reliable results.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Ziyin Yang,Xiao Bai
This paper reports the synthesis of S-doped thin Co 3 O 4 shell with Au flower as core for nonenzymatic glucose sensing, where Au@Co 3 O 4 was obtained through assembly process triggered by redox reaction of Co 2+ and Au 3+ , and then S was doped into Co 3 O 4 shell via a hydrothermal process. The morphology, composition and structure of Au@Co 3 O 4 -S were characterized by transmission electron microscopy and X-ray diffraction, which showed that the core–shell structure was composed with Au core flower and S-doped thin Co 3 O 4 shell. Electrochemical property of Au@Co 3 O 4 -S toward glucose was explored, and an excellent electrocatalytic performance with a wide linear range of 0.2 μM–3.1 mM, a high sensitivity of 1127.3 μA mM −1 cm −2 and a low detection limit of 0.09 μM (S/N = 3) was obtained, which may be due to that the low conductivity of Co 3 O 4 was mitigated by conductive Au core flower and S doping, therefore making thin Co 3 O 4 shell provide more effective active sites for glucose reaction. Considering such excellent results, Au@Co 3 O 4 -S was proven as a promising material for electrochemical sensing of glucose.
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Shasha Feng,Fubin Pei,Yi Wu,Jingjing Lv,Qingli Hao,Tinghai Yang,Zhaoyang Tong,Wu Lei
A novel ratiometric fluorescent sensor based on Förster resonance energy transfer (FRET) platform was designed for riboflavin (RF) detection. The graphitic carbon nitrides quantum dots - Zn-MOF composite (g-CNQDs@Zn-MOF) was used as the fluorescent probe . In the FRET system, g-CNQDs@Zn-MOF and RF acted as donor and acceptor, respectively. The probe exhibited high sensitivity and good selectivity to RF, and had been successfully used for the detection of RF in milk and vitamin B 2 tablets. The detection limit of the sensor was 15 nM. The strategy expanded the application of MOF in sensing filed and provided a new method for the detection of RF.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2020
·
IF 4.613
Qingxiao An,Qinlu Lin,Xiaohui Huang,Rongju Zhou,Xin Guo,Weizhen Xu,Suyan Wang,Dong Xu,Huan-Tsung Chang
Preparation of carbon dots (C dots) with strong emission at long wavelengths is an ongoing goal to their advanced applications, especially in bioanalysis. For sensing Fe 3+ and ascorbic acid (AA), we prepared C dots with a large Stokes shift using o- phenylenediamine (OPD) as the carbon source through an electrochemical method . During the electrolysis, OPD undergoes polymerization, carbonization , and passivation at the platinum anode to form C dots. These C dots were characterized by UV–Vis spectroscopy, transmission electron microscopy , Raman spectroscopy , X-ray photoelectron spectroscopy (XPS), and FT-IR spectroscopy. They are composed of C, N, O, H elements, having a maximum of emission at 570 nm and a supreme excitation at 261 nm, with a Stokes shift of 309 nm. A new emission at 350 nm emerged with their exposure to Fe 3+ and the intensity rises upon increasing Fe 3+ , while the emission intensity at 570 nm decreases. Thus, dissimilar to the usual quenching strategy, a novel ratiometric fluorescent sensor based on the fluorescence intensity ratio (F 350 /F 570 ) of the C dots at 350 nm and 570 nm has been developed for quantitation of Fe 3+ with a limit of detection (LOD) of 0.16 μM and high selectivity toward Fe 3+ against K + , Cu 2+ , and Fe 2+ . With evidence of decreased C–N C and C–O/N–O in XPS and increased content of C O/N O and N–H, we suggested the complexation of Fe 3+ with the surface groups led to the changes in chemical bonds accounting for their optical property variation. Furthermore, AA can quench the fluorescence of C dots at 570 nm and Fe 3+ -treated C dots (incubated with 50 μM Fe 3+ ) at 350 nm by a static mechanism. Accordingly, two assays toward AA were developed with LODs of 0.12 μM and 0.32 μM by using C dots and Fe 3+ -treated C dots, respectively. Especially, both AA assays exhibited excellent selectivity under various substances including those with strong reducing ability such as pyrogallic acid, sodium citrate, tea polyphenol, and dithiothreitol. The applications of the assays were validated with the quantitation of Fe 3+ in urine and AA in beverages. Electrochemical method is a significant approach to develop novel C dots even using the same carbon sources as in other methods.
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2020
·Vol. 146
·期 1
·207-212
·
IF 3.978
Wenjie Jing,Xiangkun Cui,Fanbo Kong,Wei Wei,Yunchao Li,Louzhen Fan,Xiaohong Li
Identifying the species and concentrations of antioxidants is really important because antioxidants play important roles in various biological processes and numerous diseases. Compared with an individual sensor detecting a single antioxidant with limited specificity, a sensor array could simultaneously identify various antioxidants, in which 3–5 types of nanomaterials with peroxidase-like activity are absolutely necessary. Herein, as a single-atom nanozyme, Fe–N/C with oxidase-mimicking activity was applied to construct a triple-channel colorimetric sensor array: (1) Fe–N/C catalytically oxidized three substrates 3,3′,5,5′-tetramethylbenzidine (TMB), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and o-phenylenediamine (OPD) to produce blue oxidized TMB (oxTMB), green oxidized ABTS (oxABTS) and yellow oxidized OPD (oxOPD), respectively; (2) with oxTMB, oxABTS and oxOPD as three sensing channels, a colorimetric sensor array was constructed for simultaneously discriminating glutathione (GSH), L-cysteine (L-Cys), ascorbic acid (AA), uric acid (UA), and melatonin (MT), even quantifying concentrations (with GSH as a model analyst). The performance of the sensor array was validated through accurately identifying 15 blind samples containing GSH, L-Cys, AA, UA and MT in buffer solution and human serum samples, and also in binary and ternary mixtures. This work proved that fabricating a single nanozyme-based sensor array was a simplified and reliable strategy for simultaneously probing multiple antioxidants.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）