FOOD CHEMISTRY
·
2021
·
IF 7.514
Yu-gang Shi,Lai Jiang,Shan Lin,Wen-gang Jin,Qing Gu,Yue-wen Chen,Ke Zhang,Rammile Ettelaie
Pseudomonas fluorescens is a Gram-negative spoilage bacterium and dense biofilm producer, causing food spoilage and persistent contamination. Here, we report an ultra-efficient photodynamic inactivation (PDI) system based on blue light (BL) and octyl gallate (OG) to eradicate bacteria and biofilms of P. fluorescens . OG-mediated PDI could lead to a > 5-Log reduction of viable cell counts within 15 min for P. fluorescens . The activity is exerted through rapid penetration of OG towards the cells with the generation of a high-level toxic reactive oxygen species triggered by BL irradiation. Moreover, OG plus BL irradiation can efficiently not only prevent the formation of biofilms but also scavenge the existing biofilms. Additionally, it was shown that the combination of OG/poly(lactic acid) electrospun nanofibers and BL have great potential as antimicrobial packagings for maintaining the freshness of the salamander storge. These prove that OG-mediated PDI can provide a superior platform for eradicating bacteria and biofilm.
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FISH & SHELLFISH IMMUNOLOGY
·
2023
·
IF 4.7
Fei Sun,Yingbo Wu,Yuanxing Zhang,Qin Liu,Qiyao Wang,Xiaohong Liu
Large yellow croaker ( Larimichthys crocea ) farm industry in China suffered from huge economic loss caused by Pseudomonas plecoglossicida infection. Due to multi-antibiotic resistance, efficient vaccines are urgent to be developed to combat this pathogen. In this study, an inactivated vaccine was developed with an aluminium adjuvant (Alum) plus ginseng stem and leaf saponins (GSLS). As a result, the relative percentage survival (RPS) against P. plecoglossicida was up to 67.8 %. Comparatively, RPS of groups that vaccinated with only inactivated vaccine and vaccine containing Alum or Montanide™ 763A as adjuvant were 21.8 %, 32.2 % and 62.1 %, respectively. Assays for total serum protein and serum lysozyme activity in group vaccinated with inactivated vaccine plus Alum + GSLS adjuvant were significantly higher than that in control group. Moreover, specific antibody in serum elicited a rapid and persistent level. According to the expression of some immune related genes , inactivated vaccine plus Alum + GSLS adjuvant induced a stronger cellular immune response which was vital to defend against P. plecoglossicida . In conclusion, our study demonstrated that the compound Alum and GSLS adjuvant is a potential adjuvant system to develop LYC vaccine.
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BIOCHEMICAL PHARMACOLOGY
·
2023
·
IF 5.8
Weibin Zhang,Zhe An,Yuqi Bai,Ying Zhou,Fangyi Chen,Ke-Jian Wang
Antimicrobial resistance (AMR) constitutes a significant global threat to human health. In recent years, there has been a concerning surge in infections caused by multidrug-resistant bacteria, highlighting the pressing need to urgently explore novel and effective alternatives to conventional antibiotics. Antimicrobial peptides (AMPs) have emerged as a focal point of research, capturing significant attention as promising antimicrobial agents. In this study, we have identified a novel cationic antimicrobial peptide (AMP) named Scyreptin 1-30 , derived from the marine invertebrate Scylla paramamosain . The results showed that Scyreptin 1-30 exhibits a broad-spectrum antimicrobial activity, demonstrating significant potency against both bacteria and fungi, and even against the clinically isolated multidrug-resistant bacteria Pseudomonas aeruginosa . Moreover, Scyreptin 1-30 exhibited rapid bactericidal kinetic. The results of antibacterial mechanism showed that Scyreptin 1-30 destroyed the integrity of bacterial membranes, leading to bacterial death and exhibited potent anti-biofilm activity against P. aeruginosa . The activity of Scyreptin 1-30 against bacteria had a favorable thermal stability, displayed a certain ion tolerance, and showed no discernible cytotoxicity when assessed against both the mammalian cell line HEK293T and the fish cell lines ZF4. In an In vivo study, Scyreptin 1-30 exhibited a remarkably reduction in the bacterial load caused by multidrug-resistant P. aeruginosa at the site of infection, and promoted wound healing in a mouse model of burn infection. This study indicated that Scyreptin 1-30 holds promise as an effective antibacterial agent, potentially serving as a topical skin treatment against multidrug-resistant bacterial infections, including those caused by P. aeruginosa.
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JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
·
2024
·
IF 2.5
Lu Huang,Ye Zhang,Jie Liu,Dalin Zhang,Li Li
This study presents a fluorescent mechanism for two-step amplification by combining two widely used techniques, exponential amplification reaction (EXPAR) and catalytic hairpin assembly (CHA). Pseudomonas aeruginosa ( P. aeruginosa ) engaged in competition with the complementary DNA in order to attach to the aptamer that had been fixed on the magnetic beads. The unbound complementary strand in the liquid above was utilized as a trigger sequence to initiate the protective-EXPAR (p-EXPAR) process, resulting in the generation of a substantial quantity of short single-stranded DNA (ssDNA). The amplified ssDNA can initiate the second CHA amplification process, resulting in the generation of many double-stranded DNA (dsDNA) products. The CHA reaction was initiated by the target/trigger DNA, resulting in the release of G-quadruplex sequences. These sequences have the ability to bond with the fluorescent amyloid dye thioflavin T (ThT), generating fluorescence signals. The method employed in this study demonstrated a detection limit of 16 CFU/ml and exhibited a strong linear correlation within the concentration range of 50 CFU/ml to 10 5 CFU/ml. This method of signal amplification has been effectively utilized to create a fluorescent sensing platform without the need for labels, enabling the detection of P. aeruginosa with high sensitivity.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jinyue Sun,Hui-Ling Shen,Jia-Neng Pan,Ting Yu,Wen-Wen Zhou
The ferrous sulfate (FeSO 4 )-based mechanism causing ferroptosis-like death in Pseudomonas aeruginosa was investigated. FeSO 4 triggered ferroptosis in P. aeruginosa planktonic cells, decreased the ratio of glutathione to oxidized glutathione, and resulted in the increase of reactive oxygen species and lipid peroxidation, damaging the integrity of the cell membrane. In addition, FeSO 4 prevented P. aeruginosa from forming biofilms on the surface of stainless steel, glass, and high-density polyethylene. Transcriptome analyses indicated that there were 412 up-regulated genes and 782 down-regulated genes following FeSO 4 treatment. FeSO 4 increased the cross-linking density of a carboxymethyl chitosan (CMCS) agar-based film, reducing its water solubility, swelling degree, water vapor permeability, and oxygen permeability. Finally, FeSO 4 @CMCS agar-based film showed potential antibacterial ability against the growth of P. aeruginosa in grapes, purple kale, and cherry tomatoes during storage.
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Foods
·
2025
·Vol. 14
·期 3
·531
·
IF 5.1
Taizhi Shen,Changrong Cao,Ruiyu Zhu,Jian Chen,Feifei Wang,Yanbo Wang
Pseudomonas fluorescensis a vital food spoilage bacterium that commonly spoils foods in the biofilm state. Uncovering the targets responsible for biofilm formation and disrupting their function is a promising way to control bacterial biofilms and food spoilage. In this work, using the combination of qRT-PCR and construction of the gene deletion strain, Δtdsr, TonB-dependent siderophore receptor D7M10_RS23410 was, for the first time, proven to play an essential part in the biofilm development ofP. fluorescens. By utilizing structure-based virtual screening technology, a natural compound, adenosine monophosphate (AMP), with the highest binding activity to D7M10_RS23410, was obtained as an effective biofilm inhibitor. AMP significantly decreased the cell autoaggregation and biofilm biomass at sub-MIC concentrations (2.5, 1.25, and 0.625 mg/mL), mainly through inhibiting the generation of extracellular polymeric substances (EPS) in the biofilm matrix and promoting the cell motility. Furthermore, AMP was found to form hydrogen bonds with specific amino acid residues and stretched the protein structure of D7M10_RS23410, and this structural alteration undoubtedly interfered with the functionality of the D7M10_RS23410 protein.
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FOOD MICROBIOLOGY
·
2022
·
IF 6.374
Jian Chen,Rendi Yang,Yongzheng Wang,Shigenobu Koseki,Linglin Fu,Yanbo Wang
In this study, we found the inhibitory effect of d -Tryptophan ( d -Trp) on the bacterial growth and spoilage potential of P. fluorescens and S. baltica . The effectiveness of d -Trp in bacterial growth inhibition was highly dependent on environmental NaCl concentrations. The growth/no growth models were developed to clarify the relationship between salt and d -Trp concentrations for optimal growth-inhibitory effect. It showed that higher levels of NaCl (>3.5%) with d -Trp (>15 mM) are required for growth inhibition. The d -Trp treatment combined with NaCl also retarded the psychrotrophic growth and reduced the respiratory active bacteria at low temperature, indicating that the inhibitory effect of d -Trp might be associated with the tolerance to osmotic and cold stress. Furthermore, the treatment d -Trp combined NaCl (artificial seawater) on salmon fillets was investigated. During refrigerated storage, the d -Trp treated salmon fillet either inoculated with S. baltica or P. fluorescen maintained remained a relatively low level of total volatile basic nitrogen (TVBN) (below 30 mg N/100 g during 8-days storage) and delayed increase of low total viable bacteria counts (TVC) with a longer lag phase of 2 days.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Jing Liu,Yu-Jing Wang,Ren-Jie Jin,De-Ke Li,Fan-Lin Zeng,Siming Yu,Wei-Min Chen,Jing Lin
A triple-functional mPDA-SNO@5 h nanoplatform integrating PTT, NO therapy, and c -di-GMP pathway intervention is developed. • The nanoplatform achieves on-demand release of NO and 5 h under NIR or bacterial microenvironment stimuli. • Synergistic action of NO and 5 h enables both mature biofilm dispersal and sustained biofilm inhibition. • Combination of PTT and high-concentration NO achieves efficient eradication of biofilm-embedded persister bacteria. Drug resistance and recurrent chronic infection of Pseudomonas aeruginosa caused by biofilm formation are becoming increasingly serious. Particularly for infections caused by the clinically common hyper-biofilm phenotype mutant strain PAO1-Δ wspF . Traditional antibiotics alone are often insufficient to overcome mature biofilms and eradicate the bacteria within them, making it difficult to achieve the desired antibacterial effect. In this study, we designed an antibacterial nanoplatform mPDA-SNO@5 h, which can gradually release nitric oxide (NO) and cyclic diguanylate ( c -di-GMP) G-quadruplex inducer 5 h at physiological temperature, thereby interfering with the c -di-GMP signal pathway to eradicate and persistently inhibit the stubborn biofilm of PAO1-Δ wspF . Furthermore, under 808 nm near-infrared (NIR) light irradiation, the potent photothermal therapy (PTT) effect of mPDA-SNO@5 h, combined with the rapid release of a substantial amount of NO under light exposure, synergistically exerted exceptional antibacterial effects. In a mouse model of skin wound infection, this nanoplatform showed excellent antibacterial and wound-healing properties without inducing obvious toxic and side effects. The findings of our work provide a potential strategy for eradicating clinical biofilm-associated drug-resistant infections caused by Pseudomonas aeruginosa . Download: Download high-res image (77KB) Download: Download full-size image
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BIORESOURCE TECHNOLOGY
·
2013
·
IF 4.75
Dong-Zhi Chen,Jun-Yi Fang,Qian Shao,Jie-Xu Ye,Du-Juan Ouyang,Jian-Meng Chen
A novel entrapment matrix, calcium alginate (CA) coupled with activated carbon fiber (ACF), was prepared to immobilize Pseudomonas oleovorans DT4 for degrading tetrahydrofuran (THF). The addition of 1.5% ACF increased the adsorption capacity of the immobilized bead, thus resulting in an enhanced average removal rate of 30.3 mg/(L h). The synergism between adsorption and biodegradation was observed in the hybrid CA–ACF beads instead of in the system comprising CA beads and freely suspended ACF. The effective diffusion coefficient of the CA–ACF bead was not significantly affected by bead size, but the bead’s value of 1.14 × 10 −6 cm 2 /s (for the bead diameter of 0.4 cm) was larger than that of the CA bead by almost one order of magnitude based on the intraparticle diffusion–reaction kinetics analysis. Continuous treatment of the THF-containing wastewater was succeeded by CA–ACF immobilized cells in a packed-bed reactor for 54 d with a >90% removal efficiency.
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Pharmaceuticals
·
2025
·Vol. 18
·期 6
·817
·
IF 4.8
Luqi Qin,Yixia Zhou,Bei Fan,Jiahuan Zheng,Rao Diao,Jiameng Liu,Fengzhong Wang
Background/Objectives: Endophytes can produce bioactive metabolites similar to their host plants. CM-YJ44 ( Pseudomonas protegens CHA0, 99.24% similarity), an endophyte from Dendrobium officinale , has not yet validated hypoglycemic potential. This study aimed to evaluate its anti-insulin resistance (IR) activity and metabolite profile. Methods: The fermentation broth of CM-YJ44 was separated into three fractions (CM-YJ44-1, -2, and -3) using semi-preparative high-performance liquid chromatography (pre-HPLC). An IR HepG2 cell model was constructed to evaluate their glucose uptake capacity. CM-YJ44-3 was further tested for oxidative stress, inflammatory, and insulin signaling pathway activation. Metabolites in CM-YJ44-3 were preliminarily identified using the Q Exactive Focus LC-MS system (QE), and the dendrobine content was quantified by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS). Molecular docking was performed to predict the binding affinities between dendrobine and target proteins. Results: Among the three fractions, CM-YJ44-3 significantly reduced nitric oxide (NO) and reactive oxygen species (ROS) levels in IR cells, enhanced glycogen synthesis, upregulated the activities of pyruvate kinase (PK) and hexokinase (HK), and suppressed the expression of inflammatory factors. Its mechanism of action was mainly through activation of the IRS1/PI3K/Akt/GSK3β/GLUT4 signaling pathway. QE analysis preliminarily identified 24 metabolites in CM-YJ44-3. Quantitative analysis by UPLC-MS/MS showed that the dendrobine content was 78.73 ± 4.29 ng/mL. Molecular docking results indicated that dendrobine exhibited binding energies below −5 kcal/mol with multiple target proteins involved in this signaling pathway, suggesting it may be a key bioactive component responsible for the anti-IR effect. Conclusions: This study provides the first evidence of hypoglycemic bioactive metabolite production by strain CM-YJ44, indicating its potential as a novel microbial candidate for alleviating IR.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Tao Tang,Min Xue,Fanfan Wang,Nengneng Xie,Houyun Huang,Yuanyuan Duan,Xiaoyue Wang,Wuxian Zhou,Qingfang Wang,Jingmao You
Pseudomonas fluorescens HT1, isolated from the rhizosphere soil of Coptis chinensis, exhibits potent antagonistic activity against the causal agent of southern blight, Sclerotium rolfsii. This study investigated that the VOCs produced by P. fluorescens HT1 markedly inhibited mycelial growth by 88.31% and sclerotial germination by 79.17% of S. rolfsii LC1. Gas chromatography–mass spectrometry (GC–MS) analysis identified key VOC constituents, namely, chloromethyl methyl sulfide, propionic anhydride, 2-methyl-5-(methylthio)-furan, 2-decanone, 2-undecanone, isobutyl benzoate, and 3-cyclohexyl-1-propanol, demonstrating potent antifungal activity. RNA-seq analysis further revealed that the VOCs markedly altered the transcriptome of S. rolfsii LC1, specifically downregulating genes associated with growth factor and transmembrane transport. In addition, P. fluorescens HT1 successfully colonized C. chinensis tissues and enhanced host resistance against S. rolfsii. This study elucidated the biocontrol mode of action of P. fluorescens HT1 and provides a basis for developing VOC-based management strategies to manage soilborne southern blight in C. chinensis.
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BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Qian Feng,Laiwei Luo,Xindi Chen,Kaijie Zhang,Fang Fang,Zhaoxia Xue,Chao Li,Jiashun Cao,Jingyang Luo
The N-Acy-L-homoserine lactones (AHLs) mediated quorum sensing (QS) system exhibited important ecological significance in bacterial biofilm formation. However, the previous studies mainly focused on indigenous AHLs while the role of exogenous AHLs has remained unclear. This study evaluated the roles of exogenous AHLs on the biofilm formation of Pseudomonas aeruginosa . Both the C6-HSL and C8-HSL promoted the biofilm formation of P. aeruginosa with an enhancement of 2.47 and 1.88 times, respectively. Further analysis showed that exogenous AHLs contributed greatly to the adhesive ability instead of growth rate. Also, the bacterial motility and metabolic activities were significantly improved by AHLs. Moreover, the microbial functional genes ( i.e. lasI , lasR , rhlI and rhlR ) involved in regulating the biofilm formation were highly expressed in AHLs reactors. These findings expanded the knowledge of AHLs functions in mediating biofilm formation, and provided insightful guidance on the biofilm regulation in the wastewater treatment via biofilm technology.
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