Materials Today Bio
·
2025
·
IF 10.2
Yaxin Wang,Fang Luo,Pengcheng Zhang,Hong Niu,Yaomin Tan,Yizhi Zhang,Ziyan Tang,Lina Du,Yiguang Jin,Xu Jin
Neuroinflammation is a key pathogenic process in multiple central nervous system (CNS) disorders. It can lead to neuronal injury and cognitive decline through excessive glial activation and aberrant engagement of the programmed cell death protein-1/programmed death-ligand 1 (PD-1/PD-L1) checkpoint axis. To address these pathologies, we engineered a PD-1-enriched macrophage-membrane, lactoferrin-modified, PEGylated, glycyrrhizic-acid–loaded biomimetic hybrid liposome (PMLpGL) for dual, precise modulation of the neuroinflammatory microenvironment. PMLpGL alleviates neuronal inhibitory signaling by reversibly sequestering excess PD-L1 via membrane-anchored PD-1, while its cargo GA suppresses high-mobility group box-1 (HMGB1)-driven inflammatory cascades, thereby returning inducible PD-1/PD-L1 expression and glial activation toward homeostasis. Physicochemical characterization showed a hydrodynamic diameter of 165 ± 3 nm and a zeta potential of −10.2 ± 0.2 mV. Engineered macrophage membranes displayed marked PD-1 overexpression, and ligand-depletion saturation assays demonstrated specific, saturable PD-1/PD-L1 binding. In a Transwell blood–brain barrier (BBB) model, PMLpGL achieved a 24-h permeability of 22.86 ± 0.14 %, indicating robust in-vitro BBB traversal. In vivo fluorescence imaging showed peak brain accumulation at 24 h with retention to 48 h; liquid chromatography–tandem mass spectrometry further confirmed brain targeting and persistence—at 12 h, brain GA with PMLpGL was ∼48-fold higher than free drug and remained quantifiable at 48 h. Pharmacodynamic evaluations in cells and mice demonstrated that PMLpGL suppresses glial activation and normalizes inducible checkpoint expression; reshapes the cytokine milieu by lowering IL-6, IL-1β, TNF-α, and HMGB1 while increasing IL-10, TGF-β, and brain-derived neurotrophic factor; and restores the synaptic protein synapsin-1. Correspondingly, PMLpGL significantly improved cognition in open-field, novel object recognition, and Morris water maze tests. Collectively, PMLpGL combines PD-1 decoy sequestration with GA-mediated upstream immunomodulation to attenuate neuroinflammatory cascades, protect neurons, and reverse cognitive deficits. By pairing BBB compatibility with microenvironment-precise regulation, this platform offers a promising therapeutic strategy for CNS diseases associated with cognitive decline.
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高影响因子
FUEL
·
2025
·
IF 7.5
Kaiwei Wang,Hao Ruan,Fumin Wang,Yi Zhai,Mingshuai Sun,Yan Chen,Jiaxing Zhang,Yihao Wang,Xuyang Zou,Xubin Zhang
The construction of abundant TiO 6 active sites within the zeolite framework is essential for further enhancing the catalytic performance of TS-1 zeolite. Here, we employ a strategy involving treatment with a mixture of amines and ammonium salts to generate abundant TiO 6 species within zeolites synthesized with the assistance of N,N-dimethylformamide (DMF). It was observed that during the post-treatment process, the presence of amines and ammonium salts plays a key role for the formation of highly active TiO 6 species. The incorporation of DMF can significantly enhance the TiO 6 content in the post-treated zeolite, increasing it to 61.5 % compared with 28.5 % in the zeolite synthesized without DMF. The effect of the DMF/SiO 2 ratio in the synthesized gel on the morphology and catalytic performance was also examined. It is noteworthy that the incorporation of DMF influences the microenvironment of the Ti active sites following post-treatment. 1 H MAS NMR analysis reveals that the characteristic peaks corresponding to Ti-OH and isolated Si-OH groups in the DMF-synthesized catalyst are significantly intensified after treatment with amines and ammonium salts. When H 2 O 2 is employed as an oxidant, the optimal catalytic performance achieves a cyclohexene conversion of 51.1 % and a cyclohexene oxide yield of 39.4 %, with a high TOF value of 92.77 h −1 . Notably, the catalyst exhibits a significantly conversion rate of 79.4 % and a yield of 72.7 % using O 2 as the oxidant.
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Plants-Basel
·
2025
·Vol. 14
·期 24
·3718
·
IF 4.1
Xinxin Li,Ziting Zhu,Jing Wu,Nianjun Yu,Huiqun Xie,Lan Han,Daiyin Peng
Dendrobium huoshanense , a rare and endangered medicinal orchid species endemic to Huoshan County, Anhui Province, faces a severe limit of wild resources. Its medicinal efficacy derives from unique chemical constituents, which vary significantly across cultivation modes. We systematically compared sensory properties and nutritional constituents (polysaccharides, flavonoids, alkaloids, bibenzyls, minerals, and free amino acids) between D. huoshanense in three cultivation modes—greenhouse cultivation, understory gravel cultivation, and wild-simulated cultivation. Additionally, the gastroprotective effects of wild-simulated cultivated D. huoshanense on gastric mucosal epithelial cells were evaluated. Results demonstrated that wild-simulated cultivation yielded significantly higher levels of nutritional constituents compared to gravel and greenhouse cultivation. Furthermore, wild-simulated cultivated D. huoshanense exhibited notable protective effects against gastric mucosal epithelial cell damage. This study not only elucidates the influence of cultivation modes on the chemical profile of D. huoshanense but also provides scientific evidence supporting its gastric protective properties, offering a foundation for its sustainable utilization and quality-oriented cultivation.
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高影响因子
MARINE POLLUTION BULLETIN
·
2025
·
IF 4.9
Jia Shi,Dan Xu,Qiao Ma
p -Chloro- m -xylenol (PCMX), a widely used antimicrobial agent, poses potential ecological risks due to its ubiquitous distribution and documented toxicity to aquatic vertebrates. Although microbial degradation represents a key natural attenuation pathway, the molecular mechanisms governing PCMX biodegradation remain elusive. Here, we report the isolation of three novel Rhodococcus strains from marine sediments capable of mineralizing PCMX. Among these, strain R. ruber SJ-1 completely metabolized 30 mg/L PCMX within 72 h and maintained significant degradation capacity under high-salinity stress. Integrated genomic and transcriptomic analyses revealed that PCMX exposure triggered extensive transcriptional reprogramming in strain SJ-1, with 1063 genes upregulated and 533 downregulated. PCMX inhibited core cellular functions, including key genes related to cell stress resistance, energy production, central carbon metabolism, fatty acid metabolism, and protein biosynthesis. Concurrently, aromatic catabolism pathways were markedly induced. A highly upregulated gene cluster ( sj4821 - sj4838 ) was identified as pivotal for PCMX metabolism, encompassing a flavin-dependent monooxygenase (NphA1-sj, sj4831 ; 2145.26-fold) and catechol 2,3-dioxygenase (C23O, sj4838 , 2052.43-fold) genes. Quantitative PCR assays further validated the upregulation patterns. Based on bioinformatics and high-resolution mass spectrometry analyses, we proposed that strain SJ-1 converted PCMX to 4-chloro-3,5-dimethylcatechol via ortho -hydroxylation, followed by meta -cleavage of the aromatic ring. This study presents the first documented evidence of PCMX degradation by a marine Rhodococcus isolate. Our findings reveal the molecular mechanisms driving PCMX detoxification and cellular adaptation, establishing a foundation for bioremediation in saline ecosystems.
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高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2025
·Vol. 36
·期 7
·420
·
IF 2.8
Chen Yuan-Bin,Tang Ling
A solid-state reaction method was successfully employed to synthesize [(Zn 0.8 Mg 0.2 ) 1-x Ni x ] 2 SiO 4 microwave dielectric ceramics by partially substituting [Zn 0.8 Mg 0.2 ] 2+ with Ni 2+ ions. XRD analysis indicated that the proportion of the primary Zn 2 SiO 4 phase decreases with increasing Ni 2+ content, while the secondary MgNi(SiO 4 ) phase becomes predominant. This phase transition contributes to a reduction in dielectric loss. The sintering behavior, phase composition, microstructure, and microwave dielectric properties of the ceramics were systematically investigated. The incorporation of Ni 2+ ions lowered the optimal sintering temperature from 1325 to 1400 °C. SEM analysis revealed that an optimal Ni 2+ substitution level enhanced the grain density within the [(Zn 0.8 Mg 0.2 ) 1-x Ni x ] 2 SiO 4 ceramic matrix. The relationship between Q × f and the average grain size as well as grain uniformity was analyzed. The trend of τ f is primarily governed by the total V izn-o and E zn-o . Additionally, the relationship between lattice vibrations, Raman shifts, and dielectric properties was investigated using Raman spectroscopy. Certainly, the [(Zn 0.8 Mg 0.2 ) 0.8 Ni 0.2 ] 2 SiO 4 ceramic, sintered at 1325 °C, exhibits an exemplary set of microwave dielectric properties: an εr value of 6.8, a Q × f value of 27,185 GHz, and a τ f value of − 37 ppm/ °C.
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高影响因子
JOURNAL OF FISH BIOLOGY
·
2024
·
IF 1.7
Leya Qu,Liping Yang,Yiran Wang,Shaoyang Zhi,Mengjuan Zhao,Jinrui Xiong,Xiao Yan,Chaobin Qin,Guoxing Nie
Isthmin-1 (Ism1) plays roles in glucose uptake in mammals as an adipokine. To investigate its role in the glucose metabolism of common carp ( Cyprinus carpio. L), the Ism1 sequence was cloned, and its expression and distribution in tissues were detected. In addition, we prepared and purified the recombinant Ism1 protein using the E. coli expression system and assessed changes in the expression of key genes related to glucose metabolism through both in vivo injection experiments and primary hepatocyte experiments in vitro. The results revealed that the open reading frame of Ism1 was 1377 bp long, encoding 458 amino acids. Similarity analysis indicated that Ism1 exhibited a close evolutionary relationship with goldfish ( Carassius auratus ), sharing 98.35% amino acid similarity. Ism1 was expressed in all tissues of common carp, with the highest level observed in the heart, followed by the gill, head kidney, and hepatopancreas. Distinct patterns of Ism1 expression were identified during the oral glucose tolerance test and long-term high-carbohydrate and high-fat diet feeding experiments. In vivo studies demonstrated that the serum glucose concentration was reduced on treatment with Ism1, accompanied by a significant upregulation of mRNA levels for gk , hk , and pfk genes in hepatopancreas; conversely pepck and g6pase mRNA levels were significantly downregulated in the hepatopancreas under these conditions as well. Furthermore, our primary hepatocyte experiment confirmed that Ism1 could inhibit pepck and g6pase mRNA expression, while promoting gk , hk , and pfk mRNA expression levels. In conclusion, Ism1, in common carp, could participate in the glucose metabolism, which provides essential information for future studies on the function of Ism1.
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高影响因子
JOURNAL OF COORDINATION CHEMISTRY
·
2017
·
IF 1.795
Shan-Shan Zhao,Peng Wang,Li-Na Wang,Lin Fu,Xiao-Meng Han,Jie Qin,Shao-Song Qian
The multidentate Schiff-base ligand N′-(1-(pyrazin-2-yl)ethylidene)isonicotinohydrazide (HL) has been prepared. Reaction with zinc, copper, and silver nitrate afford three complexes, [Zn(HL′)2](NO3)2·3H2O (1), {[Cu2(L)2(NO3)(H2O)2]·NO3}n (2) and {[Ag2(L)2]·3H2O}n (3). These complexes have been characterized by elemental analysis, IR spectroscopy, and single-crystal X-ray diffraction. In 1, HL is a neutral tridentate ligand, whereas in 2 and 3, HL is a deprotonated tetradentate ligand. The hydrogen bonding interactions between NO3− and the host framework result in various supramolecular polymeric structures: a 2-D layer for 1 and 3-D network for 2 and 3. The antibacterial activities of these complexes have been investigated and the results indicate that 3 showed good antibacterial activities.
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高影响因子
JOURNAL OF VIROLOGY
·
2018
·Vol. 92
·期 17
·
IF 4.368
Cong-Cong Wu,Xuan Jiang,Xian-Zhang Wang,Xi-Juan Liu,Xiao-Jun Li,Bo Yang,Han-Qing Ye,Thomas Harwardt,Man Jiang,Hui-Min Xia,Wei Wang,William J. Britt,Christina Paulus,Michael Nevels,Min-Hua Luo
The mechanisms underlying neurodevelopmental damage caused by virus infections remain poorly defined. Congenital human cytomegalovirus (HCMV) infection is the leading cause of fetal brain development disorders. Previous work has linked HCMV infection to perturbations of neural cell fate, including premature differentiation of neural progenitor cells (NPCs). Here, we show that HCMV infection of NPCs results in loss of the SOX2 protein, a key pluripotency-associated transcription factor. SOX2 depletion maps to the HCMV major immediate early (IE) transcription unit and is individually mediated by the IE1 and IE2 proteins. IE1 causes SOX2 downregulation by promoting the nuclear accumulation and inhibiting the phosphorylation of STAT3, a transcriptional activator of SOX2 expression. Deranged signaling resulting in depletion of a critical stem cell protein is an unanticipated mechanism by which the viral major IE proteins may contribute to brain development disorders caused by congenital HCMV infection.IMPORTANCE Human cytomegalovirus (HCMV) infections are a leading cause of brain damage, hearing loss, and other neurological disabilities in children. We report that the HCMV proteins known as IE1 and IE2 target expression of human SOX2, a central pluripotency-associated transcription factor that governs neural progenitor cell (NPC) fate and is required for normal brain development. Both during HCMV infection and when expressed alone, IE1 causes the loss of SOX2 from NPCs. IE1 mediates SOX2 depletion by targeting STAT3, a critical upstream regulator of SOX2 expression. Our findings reveal an unanticipated mechanism by which a common virus may cause damage to the developing nervous system and suggest novel targets for medical intervention.
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高影响因子
KOREAN JOURNAL OF CHEMICAL ENGINEERING
·
2017
·Vol. 34
·期 12
·3132-3141
·
IF 2.007
Zhang Wei,Li Xin,Wang Hong,Song Yongji,Zhang Shenghong,Li Cuiqing
Deep desulfurization of model oil by photocatalytic air oxidation and adsorption using Ti (1− x ) M
x
O 2 (M=Zr, Ce) was investigated. Ti (1− x ) M
x
O 2 (M=Zr, Ce) was prepared by urea gelation/co-precipitation method, and characterized by N 2 adsorption, XRD and UV-vis spectra. UV irradiation greatly enhanced the adsorptive capacity and selectivity of TiO 2 -ZrO 2 for organosulfur in model oil because organosulfur compounds were first photocatalytically oxidized to sulfoxides and sulfones over TiO 2 -ZrO 2 , which were then selectively adsorbed on the bifunctional material due to much higher polarities of generated sulfoxides and sulfones. The Ti/Zr molar ratio and calcination temperature were optimized to 5 : 5 and 500 °C with the sulfur removal of 99.6% after reaction for 2 h under UV irradiation. After adding 25 wt% toluene into model oil, the sulfur removal could still reach 97.2% after reaction for 7 h. TiO 2 -ZrO 2 could be well regenrated by washing with acetonitrile followed by thermal treatment in air.
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JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL
·
2015
·
IF 3.615
Yong-Qi Li,Peng Wang,Heng Zhang,Xiao-Li Zhao,Yong Lu,Zora Popović,Ye Liu
The neutral diphosphine of 1 and the corresponding ionic diphosphine of 2 after quaternization 1 by MeOTf were prepared and investigated as the wide bite angle and bulky steric ligands to control the regioselectivity in Rh(acac)(CO) 2 -catalyzed hydroformylation of 1-octene. As for 2 , due to the electrostatic repulsive force of the two positive charges, the involved two-PPh 2 were located in trans -position with the relatively longer P 1… P 2 distance [8.0231(11) Å] to prefer to aa-site coordination in trigonal bipyramid H(alkene)Rh(CO)(P–P) intermediate, which renders less bulky steric hindrance for the regioselectivity to linear aldehyde as a result of low L / B ratio of 1.9. Due to the strong electron-withdrawing effect from the positive-charged imidazolium rings on the neighbored P-atoms, 2 with strong π-acceptor ability can develop π-backdonation interaction between Rh and P linkage to favor the dissociation of CO ligand and facilitate the selectivity to nonanals. In contrast, 1 with no electrostatic repulsive effect and less steric hindrance, the two-PPh 2 were able to be conveniently located in cis -position with the relatively shorter P 1… P 2 distance [ P 1… P 2, 6.2096(10) Å], which corresponded to the preferential linear regioselectivity with L / B = 7.1. The in situ high pressure spectroscopy analysis indicated that the involvement of 1 in Rh(acac)(CO) 2 -catalyzed hydroformylation of 1-octene could greatly facilitate the formation and stability of the active Rh–H species ( ν 2047 cm −1 ).
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高影响因子
BIOORGANIC CHEMISTRY
·
2018
·
IF 3.929
Jianpeng Su,Jiayun Liu,Cheng Chen,Yuejuan Zhang,Kewu Yang
The superbug infection caused by New Delhi metallo-β-lactamase (NDM-1) has grown into an emerging threat, labelling and inhibition of NDM-1 has proven challenging due to its shuttling between pathogenic bacteria. Here, we report a potent covalent scaffold, ebsulfur, for targeting the protein in vitro and in vivo . Enzymatic kinetic study indicated that eighteen ebsulfurs gained except 1a – b and 1f inhibited NDM-1, exhibiting an IC 50 value ranging of 0.16–9 μM, and 1g was found to be the best, dose- and time-dependent inhibitor with an IC 50 of 0.16 μM. Also, these ebsulfurs effectively restored the antibacterial activity of cefazolin against E. coli expressing NDM-1, and the best effect was observed to be from 1g , 1i and 1n , resulting in an 256-fold reduction in MIC of the antibiotic at a dose of 16 μg/mL. The equilibrium dialysis study implied that the ebsulfur disrupted the coordination of one Zn(II) ion at active site of NDM-1. Labelling of NDM-1 using a constructed fluorescent ebsulfur Ebs - R suggested that the inhibitor covalently bound to the target through SDS-PAGE analysis in vitro . Also, labelling NDM-1 in living E. coli cells with Ebs - R by confocal microscopic imaging showed the real-time distribution change process of intracellular recombinant protein NDM-1. Moreover, the cytotoxicity of these ebsulfurs against L929 mouse fibroblastic cells was tested, and their capability to restore antibacterial activity of antibiotic against clinical strains E. coli EC08 producing NDM-1 was determined. The ebsulfur scaffold proposed here is valuable for development of the covalent irreversible inhibitors of NDM-1, and also for labelling the target in vitro and in vivo .
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高影响因子
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2022
·
IF 6.437
Ping Ye,Heng Zhai,Yueqi Li,Wenkun Zhu,Guangcheng Yang,Changping Guo
Breaking the inherent contradiction between high energy and insensitivity and pursuing enviro-friendly, high safety and excellent thermal decomposition properties are urgently desired for the development of modern composite solid propellants . 2,6-Diamino-3,5-dinitropyrazine-1-oxide (LLM-105) nanoparticles were uniformly deposited on the surface of ammonium perchlorate (AP) by ultrasonic shock with Estane as modifier. In the current work, it was concluded that the crystal phase and morphology of AP remained unchanged, and that complete and dense AP@LLM-105 core-shell composites were obtained at 15% LLM-105. In addition, the high temperature decomposition peak of AP@LLM-105 is advanced to 296.5 ℃ and the heat release is increased to 1271 J/g. More importantly, AP@LLM-105 also showed excellent performance in sensitivity test, with impact sensitivity H 50 and friction sensitivity as low as 39 cm and 18%. Obviously, low-pollution AP@LLM-105 with high safety performance and excellent thermal decomposition performance provides a good candidate for composite solid propellants.
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高影响因子
危险品化学品经营许可证（带存储）