JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
·
2022
·
IF 1.467
An-ping Li,Yan-ping Shi
Farfarae Flos, a frequently used traditional herbal medicine with outstanding antitussive and anti-asthmatic effects. As an herbal rich in natural flavonoids, the quantitative determination of multi-flavonoids can be helpful to comprehensively evaluate its quality. Herein, an efficient, sensitive and accurate UHPLC-MS/MS method was established to simultaneous determinate nine flavonoids (rutin, hyperin, kaempferol-3-O-rutinoside, hesperidin, luteolin, quercetin, kaempferol, apigenin, and genkwanin) in Farfarae Flos. The calibration curves of the analytes had good linearity (R2 ≥ 0.9991). Limit of detection and limit of quantitation of each analytes was in the range of 1.06 × 10−5–2.26 × 10−3 μg·mL−1 and 3.52 × 10−5–7.53 × 10−3 μg·mL−1. The relative standard deviation (RSD) of the intra-day and inter-day precision was less than 2.71% and 2.94%. The RSD of repeatability test and stability test was less than 2.35% and 2.56%. The recovery rates were in the range of 95.27%–103.60%. The method was further applied to determinate 40 batches of samples that derived from different plant parts and with definite habitat. The results of chemometric statistical analysis showed that, firstly, the content of flavonoids can effectively reflect the differences between traditional medicinal parts and impurities. Secondly, the geographical environment has impact on the quality. In conclusion, this study provides a valuable methodological reference for the quality evaluation and further resource utilization of Farfarae Flos.
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INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Guo Liu,Mingjuan Liao,Baoyan Guo,Qixin Kan,Shuangde Zhou,Konglong Feng,Wanhuang Lin,Yahui Huang,Jianyin Miao,Yong Cao
Castor ( Ricinus communis L.) meal, a by-product of castor oil production, is a high-quality protein source. Unfortunately, the toxic substance limits its application. Herein, a novel continuous phase transition extraction (CPTE) technology at pilot scale was explored for castor meal detoxification. The detoxification conditions were optimized using response surface methodology (RSM) as: treatment time of 3 h, extraction pressure of 0.16 MPa and temperature of 84.8°C. And the removal rate of allergens, hemagglutinin, and ricin reached 79.2 %, 99.8 % and 99.0 %, respectively. The protein content of castor meal increased from 38.4% to 39.0%. The nontoxicity nature of the treated caster meal was verified via an acute toxicity tests where the mean lethal dose was >5000 mg/kg body weight. Overall, CPTE offers a novel and efficient tool for castor meal detoxification, with the superiority of non-nutrient loss, it improved the application value of castor meal and reduced the risk of environmental pollution.
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JOURNAL OF INFECTIOUS DISEASES
·
2024
·
IF 5
Tian Yu,Cao Yuwen,Liu Fang,Xia Lin,Wang Chao,Su Zhaoliang
In this study, we investigated the role of the non-canonical pyroptosis pathway in the progression of lethal sepsis. Our findings emphasize the significance of non-canonical pyroptosis in monocytes/macrophages for the survival of septic mice. We observed that inhibiting pyroptosis alone significantly improved the survival rate of septic mice, and the HMGB1 A box effectively suppressed this non-canonical pyroptosis, thereby enhancing the survival of septic mice. Additionally, our cell in vitro experiments further unveil that frHMGB1, originating from LPS-carrying histiocytes, enters macrophages via RAGE, resulting in the direct activation of caspase-11 and the induction of non-canonical pyroptosis. Notably, the A Box's competitive binding with LPS thereby impedes its entry into the cell cytosol. These findings reveal potential therapeutic strategies for slowing the progression of lethal sepsis by modulating the non-canonical pyroptosis pathway.
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JOURNAL OF FLUORESCENCE
·
2022
·Vol. 32
·期 6
·2077-2086
·
IF 2.525
Yu Bo,Yuan Ruimin,He Tianzhi,Liang Lijuan,Huang Kun
The development of mercury ion selective fluorescent probe is significant because it is one of toxic heavy metals and poses great risks and hazards to human health. Herein, we develop a mercury ion-selective fluorescent probe, namely IB , based on imidazole decorated benzothiadiazole that obtained by a facile palladium catalytic C-N coupling reaction. IB exhibits high selectivity and sensitivity towards mercury ion in water with nearly 32-fold fluorescent enhancement. The detection limit is calculated to be 0.93 nmol/L. In addition, the sensing of mercury ion can be conducted in a wide pH scope ranging from 4.0 to 10.0. Subsequently, the mercury ion elicits fluorescence of IB solution can be quenched by the addition of cyanide anions, showing “off-on-off” fluorescence transformation with at least 5 cycles, demonstrating the reversible sensing ability of IB . Furthermore, an INHIBIT logical detector has been developed using mercury ion and cyanide anions as inputs and fluorescence of IB as output. Significantly, IB can be utilized for mercury ion detection in real water sample.
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ELECTROCHIMICA ACTA
·
2023
·
IF 6.6
Ji-Wei Zhang,Wen-Hao Wang,Meng-Xiang Xie,Hou-Ran Wu,Yan-Pei Wang,Hao-Tian Bao,Bo-Qun Cao,Gang-Qin Shao
The lithium silicon phosphor sulfo-chloride (LiSiPSCl) with a Li 10 GeP 2 S 12 -type structure is one of the most promising solid electrolytes (SEs) for all-solid-state lithium-ion batteries (ASSLIBs) due to its currently highest ionic conductivity at RT. In this work, three kinds of LiSiPSCl electrolytes, i.e. Li 7.59(10) Si 1.95(9) P 1.65(7) S 11.64(1) Cl 0.36(1) (SE-450), Li 7.67(12) Si 1.90(1) P 1.67(1) S 11.61(2) Cl 0.39(2) (SE-475) and Li 7.58(5) Si 1.75(4) P 1.79(2) S 11.54(4) Cl 0.46(4) (SE-500), were prepared at 450 / 475 / 500 °C for 8 h through a solid-state method. The assembling of blocking / symmetrical / semi-blocking cells and ASSLIBs, and electrochemical measurements were carried out by coupling the three SEs with two kinds of anodes (Li metal and Li-In alloy) and one cathode of LiNbO 3 -coated LiNi 0.8 Co 0.1 Mn 0.1 O 2 (NCM@LNO). Studies were focused on the relationship among chemical compositions, phase structures and electrochemical properties, as well as the electrolyte/electrode interfacial stability. Results show that LiSiPSCl electrolytes had favorable interfacial stability with Li-In anode and exhibited low polarization voltage (< 20 mV) and decomposition current (< 0.01 mA). The optimized NCM@LNO//SE-475//Li-In of ASSLIBs exhibited a stable long-term cycle performance with a capacity retention of 97.0% (128.4 mAh g − 1 ) after 50 cycles and 81.1% (107.4 mAh g −1 ) after 100 cycles at 0.1 C. In addition, it exhibited a good rate performance while its capacity retention remained 99.3% (140.6 mAh g −1 ) after 39 cycles at 0.05 / 0.1 / 0.2 / 0.5 / 0.05 C.
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APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2022
·Vol. 88
·期 6
·
IF 5.005
Ke Huang,Wei Liu,Yuanhe Li,Sha Zeng,Fang-Jie Zhao
Methylarsenate (MAs(V)) is a product of microbial arsenic (As) biomethylation and has also been widely used as an herbicide. Some microbes are able to reduce nontoxic MAs(V) to highly toxic methylarsenite (MAs(III)) possibly as an antibiotic. The mechanism of MAs(V) reduction in microbes has not been elucidated. Here, we found that the bacterium Enterobacter sp. CZ-1 isolated from an As-contaminated paddy soil has a strong ability to reduce MAs(V) to MAs(III). Using a MAs(III)-responsive biosensor to detect MAs(V) reduction in E. coli Trans5α transformants of a genomic library of Enterobacter sp. CZ-1, we identified gshA, encoding a glutamate-cysteine ligase, as a key gene involved in MAs(V) reduction. Heterologous expression of gshA increased the biosynthesis of glutathione (GSH) and MAs(V) reduction in E. coli Trans5α. Deletion of gshA in Enterobacter sp. CZ-1 abolished its ability to synthesize GSH and decreased its MAs(V) reduction ability markedly, which could be restored by supplementation of exogenous GSH. In the presence of MAs(V), Enterobacter sp. CZ-1 was able to inhibit the growth of Bacillus subtilis 168; this ability was lost in the gshA-deleted mutant. In addition, deletion of gshA greatly decreased the reduction of arsenate to arsenite. These results indicate that GSH plays an important role in MAs(V) reduction to generate MAs(III) as an antibiotic.IMPORTANCE Arsenic is a ubiquitous environmental toxin. Some microbes detoxify inorganic arsenic through biomethylation, generating relatively nontoxic pentavalent methylated arsenicals, such as methylarsenate. Methylarsenate has also been widely used as an herbicide. Surprisingly, some microbes reduce methylarsenate to highly toxic methylarsenite possibly to use the latter as an antibiotic. How microbes reduce methylarsenate to methylarsenite is unknown. Here, we show that gshA encoding a glutamate-cysteine ligase in the glutathione biosynthesis pathway is involved in methylarsenate reduction in Enterobacter sp. CZ-1. Our study provides new insights into the crucial role of glutathione in the transformation of a common arsenic compound to a natural antibiotic.
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Science Advances
·
2025
·Vol. 11
·期 33
·
IF 12.5
Xilin Bai,Chunxiao Li,Yuheng Luo,Qi’ang Gong,Jinghui Lu,Jiuzhong Yang,Yang Pan,Zhenrong Sun,André K. Eckhardt,Rui Sun,Ralf I. Kaiser,Tao Yang
The formation and characterization of hydrogen trioxide have fascinated scientists for more than a century, due to its role as a prototype model for oxygen-chain bonding and as a key transient in antibody-catalyzed oxidation reactions relevant to the origin of life. However, the abiotic formation pathways to hydrogen trioxide have remained elusive. Here, we demonstrate in laboratory simulation experiments that hydrogen trioxide effectively forms in water–molecular oxygen ice analogs at temperatures as low as 5 kelvin under exposure to proxies of galactic cosmic rays. Exploiting synchrotron vacuum ultraviolet photoionization reflectron time-of-flight mass spectrometry, hydrogen trioxide along with hydrogen peroxide and the hydroperoxyl radical was identified during the temperature-programmed desorption of the irradiated ices. This abiotic synthesis expands the oxidant inventory on interstellar grains, icy moons, and Kuiper belt objects, offering a plausible source of essential oxidizers for prebiotic chemistry in space.
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产品关联
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 20
·9887
·
IF 4.9
Qiuying Lu,Dongmiao Zhai,Yaxian Wu,Yihu Mao,Golam Jalal Ahammed,Xinzhong Zhang,Jingbo Yu,Xin Li
Glyphosate is one of the most widely used herbicides in agricultural, horticultural, and urban environments. However, its residue accumulation and oxidative damage pose serious threats to crop health and food safety. In this study, we evaluated the potential of epigallocatechin gallate, a natural polyphenol derived from tea, to alleviate glyphosate-induced stress in melon (Cucumis meloL.). LC-MS/MS analysis revealed that EGCG significantly reduced glyphosate residues in plant tissues. Transcriptome analysis indicated that glyphosate induced extensive transcriptional reprogramming, activating genes involved in detoxification and antioxidant defense. Co-treatment with glyphosate and EGCG partially mitigated this stress response and redirected gene expression toward secondary metabolic pathways, particularly flavonoid and phenylalanine biosynthesis. Under herbicide stress, EGCG restored the transcription of key flavonoid biosynthetic genes, includingPAL,C4H,CHI, andOMT. Meanwhile, EGCG also modulated the expression ofAPX,SOD, andGST, suggesting a selective effect on antioxidant systems. Co-expression network analysis identified key hub genes associated with oxidative stress and flavonoid metabolism. These findings demonstrate the dual regulatory role of EGCG in suppressing acute oxidative stress while enhancing metabolic adaptability, highlighting its potential as a natural additive for reducing herbicide residues in fruit crops.
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产品关联
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PubMed
高影响因子
Biomedical Materials
·
2022
·Vol. 17
·期 2
·025006
·
IF 4.103
Daohuan Lu,Zhiwen Zeng,Zhijie Geng,Cuiping Guo,Dating Pei,Jin Zhang,Shan Yu
Vascularization of thick hydrogel scaffolds is still a big challenge, because the submicron- or nano-sized pores seriously restrict endothelial cells adhesion, proliferation and migration. Therefore, porous hydrogels have been fabricated as a kind of promising hydrous scaffolds for enhancing vascularization during tissue repairing. In order to investigate the effects of pore size on vascularization, macroporous methacrylated hyaluronic acid (HAMA) hydrogels with different pore sizes were fabricated by a gelatin microspheres (GMS) template method. After leaching out GMS templates, uniform and highly interconnected macropores were formed in hydrogels, which provided an ideal physical microenvironment to induce human umbilical vein endothelial cells (HUVECs) migration and tissue vascularization. In vitro results revealed that macroporous hydrogels facilitated cells proliferation and migration compared with non-macroporous hydrogels. Hydrogels with middle pore size of 200–250 μm (HAMA250 hydrogels) supported the best cell proliferation and furthest 3D migration of HUVECs. The influences of pore sizes on vascularization were then evaluated with subcutaneous embedding. In vivo results illustrated that HAMA250 hydrogels exhibited optimum vascularization behavior. Highest number of newly formed blood vessels and expression of CD31 could be found in HAMA250 hydrogels rather than in other hydrogels. In summary, our results concluded that the best pore size for endothelial cells migration and tissue vascularization was 200–250 μm. This research provides a new insight into the engineering vascularized tissues and may find utility in designing regenerative biomaterial scaffolds.
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JOURNAL OF MEDICINAL CHEMISTRY
·
2023
·
IF 7.3
Meng Wang,Guoxiu Cao,Junjie Zhou,Jinyuan Cai,Xianjie Ma,Zhikun Liu,Xiaochao Huang,Hengshan Wang
Developing multitarget platinum(IV) prodrugs is an important strategy to attenuate cisplatin (CDDP) resistance in tandem with reduced toxicity. Herein, six novel ligustrazine-derived chalcones-modified platinum(IV) complexes were synthesized and evaluated for their anti-proliferative activities. Among them, 16a displayed higher cytotoxicity toward the tested cancer cell lines and lower cytotoxicity toward the human normal cells than CDDP or the combined group. Mechanistic studies revealed that 16a efficiently induced DNA damage and initiated a mitochondria-dependent apoptosis pathway. Besides, 16a significantly triggered ferroptosis by down-regulating expression levels of nuclear factor erythroid 2-related factor 2, glutathione peroxidase 4, and solute carrier family 7 member 11. Further, 16a obtained superior in vivo anti-tumor efficiency than CDDP in CDDP-resistant pancreatic cancer xenograft models but showed no significant side effects. In summary, our study suggested that 16a acts via a different anti-cancer mechanistic pathway than CDDP and may therefore encompass a novel practical strategy for cancer treatment.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Yuxuan Tian,Yaming Qi,Hongjing Han,Xinzhan Luo,Junyao Guan,Sijia Chen,Haiying Wang,Yanguang Chen,Xuzhong Gong
Tight oil is an important unconventional oil resource, but it is difficult to separate oil and water from produced liquid and make efficient use of it. Due to the stable structure of oil–water interface caused by resin, it is difficult to achieve demulsification. In this work, a series of polyether ester demulsifierswith different degree of polymerization were designed by esterification and polymerization. In addition, hydrogen peroxide was used to regulate the structure of resin to enhance the transmission of polyether ester, and the replacement of resin was strengthened through the structural design of polyether ester. The results showed that the molecular weight and dipole moment of resin oxidized by hydrogen peroxide decreased, which was beneficial to the transfer of demulsifier. With the increase of polymerization degree, the oil solubility and surface activity of polyether ester were improved, and the replacement ability of resin was strengthened·H 2 O 2 and demulsifier were used to synergistically promote demulsification, the dehydration rate could be further increased to 99.2%, and the water content in tight oil could be reduced to 0.56%.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2020
·
IF 7.489
Guangdi Nie,Yaxue Luan,Zongkui Kou,Jiangmin Jiang,Zhenyuan Zhang,Na Yang,John Wang,Yun-Ze Long
Manganese dioxide (MnO 2 ) promises for high-performance asymmetric suprecapacitors, owing to its high theoretical capacity, abundant source, and low cost. However, insufficient practically-achievable capacity and relatively narrow voltage window in alkaline electrolyte are blocking high energy density of MnO 2 -based supercapacitors , where strategies for activating its capacitive performance and widening voltage window are the top priorities to solve the bottleneck problems. Herein, both the fiber-in-tube (NCCM-FiT) and particle-in-tube (NCCM-PiT) nanostructures coulping active NiCoO x nanoparticles and conductive carbon with MnO 2 tubes have been purposely fabricated, using the electrospun nickel cobalt oxides/carbon nanofibers (NCO/CNFs) as the self-template agents for enhanced energy density of MnO 2 -based supercapacitors. These hierarchical hollow nanotubes with gradient pores and unique compositions yield excellent capacitive properties, in terms of a competitive capacity (431.7 F g −1 or 431.7 C g −1 , 0.5 A g −1 ), which is 2.7 times that of the MnO 2 nanotubes-based electrodes. A maximum energy density of 46.4 Wh kg −1 is obtained at the power density of 400 W kg −1 for the asymmetric device assembled with the NCCM-PiT-based positive electrode and the electrospun CNFs-based negative electrode. The remarkable energy density demonstrated by these hierarchical hollow nanotubes exemplifies a novel and effective design in electrode materials for the asymmetric supercapacitors (ASCs) with superior performance.
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危险品化学品经营许可证（带存储）