Nanomaterials
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2026
·Vol. 16
·期 6
·378
·
IF 4.8
Hao Li,Lin Zhang,You Long,Chao Shen,Song Zhang,Fang Chen,Nan Chen,Chenghong Huang
Paclitaxel is a first-line anticancer drug, but its low water solubility impedes bioavailability. The purpose of this study is to estalish a delivery strategy via carboxymethyl chitosan (CMCS)-encapsulated 6-deoxy-6-mercapto-β-cyclodextrins (dmβCDs)-modified concave cubic gold (CCGs) to achieve PTX release. CCGs were initially synthesized by the one-pot method and further modified by dmβCDs, the dmβCDs can effectively capture PTX molecules, followed by encapsulation with CMCS, and then prepare pH-responsive CMCS/dmβCDs/CCGs nanocarriers after lyophilization. Results indicated that desirable hexagonal CCGs with 50 ± 5 nm size can be obtained by adjusting H 2 O 2 and HClO concentration. FT-IR, Raman and XRD spectra had confirmed dmβCDs successfully grafted to the surface of CCGs. Drug loading experiments demonstrated that the nanocarrier encapsulated PTX in amorphous powder or molecular form have a capacity of 55.05 µg/mL. Drug release experiments revealed PTX release from CMCS/dmβCDs/CCGs nanocarriers carrying a typical pH-responsive profile and indicating earlier release in an acidic environment than in a neutral or alkaline environment. The proposed method can be utilized to effectually achieve high-efficiency solubilization and targeted release inside tumor cells of PTX.
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ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Wenli Lai,Qian Lin,Cunshan Zhang,Bin Chen,Jinyu Li,Lingting Wu,Maoru Lin,Hengfen Dai,Liqing Lin
A novel oxygen vacancy-rich PRT@MnO 2-x nanozyme was fabricated via a facile one-pot ultrasonic method. • The as-prepared material possesses prominent oxidase-like activity, relying on in-situ water activation rather than exogenous oxygen. • A sensitive colorimetric/fluorescent dual-mode sensing platform was constructed for β-Gal activity with low detection limits. • The developed strategy shows satisfactory performance in human serum, possessing promising clinical application potential. Vacancy engineering has been widely applied to modulate the enzyme-like activity of nanomaterials by adjusting their surface/electronic structures and creating additional active sites. Regulating oxygen vacancies in nanomaterials and elucidating their oxygen activation mechanisms are crucial for designing high-performance nanozymes. Herein, we employed KMnO 4 as the manganese source and protamine as the stabilizer to prepare transition metal manganese oxide (PRT@MnO 2-x ) rich in oxygen vacancies. The as-prepared PRT@MnO 2-x exhibited superior oxidase-like activity, which is proposed to originate from oxygen vacancy-mediated electron transfer and valence band hole-driven water oxidation to ·OH. Steady state kinetic constant (K m ) suggested that PRT@MnO 2-x had a stronger affinity toward 3,3′,5,5′-tetramethylbenzidine and possessed prominent catalytic performance (K m and V max were 0.074 mM and 0.031 mM s −1 mg −1 ). An innovative method is proposed to employ PRT@MnO 2-x nanosheets with abundant oxygen vacancies for colorimetric/fluorescent dual-mode detection of β-Galactosidase (β-Gal) activity. Notably, the OXD-like activity of PRT@MnO 2-x is largely independent of dissolved oxygen or molecular oxygen. Instead, it facilitates electron transition from the valence band to the conduction band through the form of oxygen vacancies, enabling the oxidation of water to ·OH on the valence band of PRT@MnO 2-x . The detection limits (LOD) determined by colorimetric and fluorescence analysis for β-Gal activity are 0.015 U/L and 0.054 U/L respectively, and satisfactory spiked recovery results were achieved in human serum samples. This method has the advantages of low cost, simple operation and rapid response. The dual-mode signal sensor is expected to provide more accurate and robust detection of β-Gal than the single-mode probe, and has a wider application prospect. Download: Download high-res image (203KB) Download: Download full-size image
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Food Chemistry-X
·
2025
·
IF 8.2
Wanbing Li,Wei Wang,Yue Cao,Bolong Fang,Haoze Li,Xiaole Zhao,Jine Wu,Chao Chang
This study aimed to prepare hollow salt microspheres using hydroxypropyl-β-cyclodextrin (HP-β-CD) and soybean soluble polysaccharides (SSPS) by spray drying to address the issues of moisture absorption and caking of hollow salt microspheres when using SSPS as a single wall material. Measurements using a water activity meter, electronic tongue, and scanning electron microscope showed that the SSPS/HP-β-CD hollow salt microspheres with a mass ratio of 1:1 had the best physical properties, such as low water content (2.43 %), low water activity (0.285), strong salty taste perception, complete spherical appearance, and good fluidity. This investigation involved evaluating the influence of hollow salt microspheres on dough rheology and bread quality. Notably, the application of these microspheres to achieve a 15 % reduction in sodium content enhanced both dough and bread quality attributes. This work presents an effective technical strategy for salt reduction in baked goods, thereby expanding the application of hollow salt microspheres beyond their conventional use as topical seasoning materials.
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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
·
2025
·Vol. 29
·期 19
·e70877
·
IF 4.2
Tonghao Yao,Liangliang Liu,Yibo Miao,Xinxin Li,Ying Yu,Rongyao Sun,Yining Zhang,Luping Cui,Xu Ma
Diabetes is a significant global chronic disease characterised by elevated mortality and disability rates due to persistent infections resulting from refractory wounds. Currently, effective treatment strategies are lacking. Adipose-derived stem cell extracellular vesicles (ADSC-EVs) have been shown to promote skin wound healing; however, their clinical application is impeded by low yield and heterogeneity. We successfully isolated high-yield extruded nanovesicles from adipose stem cells (ADSC-NVs), achieving yields over 30 times greater than those of ADSC-EVs while maintaining similar mor-phological characteristics. Our findings indicate that ADSC-NVs exhibit a dose-dependent en-hancement of proliferation and migration in primary human dermal fibroblasts (HDF) in vitro. Notably, the expression levels of proliferating cell nuclear antigen (PCNA), collagen type I (COL-I) and collagen type III (COL-III) were significantly upregulated in HDF following treatment with ADSC-NVs. RNA-seq analysis further revealed that the differentially expressed genes (DEGs) shared between the ADSC-NVs group and control group were predominantly enriched in the Wnt signalling pathway. Consistently, ADSC-NVs facilitate efficient diabetic wound healing while promoting proliferation and inhibiting inflammation via the Wnt/β-catenin signalling pathway. In summary, high-yield ADSC-NVs represent a promising alternative to ADSC-EVs for enhancing diabetic wound healing, providing novel insights and methodologies for improving therapeutic outcomes.
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MOLECULES
·
2025
·Vol. 30
·期 12
·2526
·
IF 4.6
Yingya Qiu,Jiangxuan Lai,Yuhan Zhang,Sheng Fang,Zili Guo,Xianrui Liang
Despite their broad pharmacological potential, the clinical application of stilbenes derived fromCajanus cajan(L.) Millsp. leaves (CCMLs) is limited by their poor aqueous solubility, resulting in low oral bioavailability. In this study, an inclusion complex of stilbenes extracted from CCMLs and 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) was successfully developed to improve their solubility and oral bioavailability. The preparation conditions were optimized using the response surface methodology, with an optimum mass ratio of CCML extract (CCMLE) to HP-β-CD of 1.0:8.2 (g/g) and an optimal temperature of 33 °C for 2 h. The maximum inclusion efficiency of stilbenes was 82%, and the physicochemical properties of the inclusion complex were characterized. Both in vitro dissolution studies and in vivo pharmacokinetic evaluation demonstrated that HP-β-CD encapsulation significantly improved the solubility and dissolution rate of stilbenes compared to those of unformulated CCMLE. Notably, the relative bioavailability of cajaninstilbene acid (CSA), longistyle C (LLC) and 4-O-methylpinosylvic acid (MPA) increased to 198%, 177% and 131%, respectively. This work provides a simple and effective strategy to enhance the solubility and bioavailability of naturally derived stilbenes, offering promising potential for the development of other insoluble natural components for both functional food and pharmaceuticals.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Song Xue,Yu Xu,Sihui Ding,Haoxiang Meng,Xiaodong Sun,Qi Zhang
Two hydrophobic (deep) eutectic solvents (H(D)ESs), menthol:octanoic acid (Men:OctA) and menthol:trioctylphosphine oxide (Men:TOPO), were prepared, characterized, and then employed for the first time in combination with sulfobutylether-β-cyclodextrin (SBE-β-CD) for chiral separations in capillary electrokinetic chromatography (EKC). The “H(D)ES + SBE-β-CD” dual pseudo-stationary phase (PSP) systems yielded not only enhanced enantioselectivities, but also accelerated migrations of primaquine and chlorpheniramine, two drugs that cannot be resolved using the single SBE-β-CD system. The enhancement originated from the combined effects of stereoselective complexation and hydrophobic partitioning involving the H(D)ES phase, which moved faster than the anionic CD phase under the separation conditions. The best results in terms of enantioresolution and migration time were obtained using a 20 mM phosphate buffer (pH 7.0) containing 2.0 % SBE-β-CD and 0.2 % Men:OctA at an applied voltage of 15 kV. We further compared the chiral EKC performance of the H(D)ESs with a series of conventional PSPs, including three micelles (Tween-20, Brij-35, SDS) and three ionic liquids (ILs). The results showed that none of these modifiers could achieve comparable EKC performance, suggesting that in certain cases, H(D)ESs could be a superior alternative to traditional modifiers for chiral EKC separations.
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Analytical Methods
·
2026
·
IF 3
Yao Tian,Mengsi Liang,Yongdong Wei,Yuxin Wang,Ning-Bo Li,Yaoyong Wang
Zearalenone (ZEN) is a carcinogenic mycotoxin commonly found in grains, cereals, and dairy products, posing cancer risks to humans and animals. Chronic high-dose ZEN exposure can cause liver disease, immunosuppression, kidney damage, and tumorigenesis. Sensitive and efficient ZEN detection is therefore essential for protecting human and animal health. To meet the need for sensitive, selective, and low-cost mycotoxin sensors in real food samples, we developed a novel ratiometric electrochemical aptamer sensor for ZEN detection. The sensor design utilizes β-cyclodextrin-functionalized graphene (CG) to effectively enhance electron transfer efficiency, combined with host–guest recognition between ferrocene (Fc) and β-cyclodextrin (β-CD), as well as Au-S covalent bonding. The 5′-SH and 3′-MB-modified dual-labeled ZEN aptamer (MB-Apt-SH) was immobilized via β-CD/Fc inclusion, with signal amplified by AuNPs binding to -SH. Without ZEN, the aptamer stays on the electrode, giving high IMB and low IFc. With ZEN, it binds the target, pulling it away. Adding Fc-COOH fills the β-CD cavities, increasing IFc and decreasing IMB, thus raising the IFc/IMB ratio proportionally to ZEN concentration. The sensor exhibits a wide linear range from 1 pg/mL to 1000 ng/mL, with a detection limit of 0.4344 pg/mL. Furthermore, the method was successfully applied to the determination of ZEN in wine samples, yielding recovery rates between 94.00% and 104.60%, demonstrating excellent accuracy and applicability. These results indicate that the MCH/AuNPs/SH-Apt-MB/CG/GCE platform is a highly sensitive and selective electrochemical sensor for ZEN detection.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 15
Ma Tianyu,Li Qiang,Chen Xingwu,Dong Jixiang,Jiang Yuqing,Li Shanshan
Bioactive peptides are promising candidates for hair regeneration, but their topical application is often limited by poor skin delivery and the need for frequent dosing. To overcome these challenges, we designed a palmitoylated peptide, Pal-MPAPO, and incorporated it into a hyaluronic acid-based hydrogel microneedle (MN) system for localized transdermal delivery. The fabricated MNs showed adequate mechanical strength for skin insertion and provided a time-dependent, prolonged release of Pal-MPAPO. In human dermal papilla cells (DPCs), Pal-MPAPO activated the Wnt/β-catenin pathway and increased intracellular cyclic adenosine monophosphate (cAMP) levels in a predominantly VPAC1-dependent manner. Additional VPAC2 validation did not reveal a significant contribution to cAMP signaling under the tested conditions. Functional interactions between Pal-MPAPO and VPAC1 were supported by molecular docking, molecular dynamics simulations, receptor knockdown, and fluorescence imaging. In a C57BL/6 mouse model, Pal-MPAPO-loaded MNs significantly promoted hair regrowth, achieving efficacy comparable to minoxidil but at a reduced application frequency. These findings identify Pal-MPAPO as a potential peptide candidate for hair regeneration and suggest that hydrogel MN-assisted delivery provides a localized peptide administration strategy. Graphical The alternative text for this image may have been generated using AI.
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MOLECULES
·
2019
·Vol. 24
·期 2
·
IF 3.06
Lili Xu,Man Wang,Qing Chen,Jiajia Yang,Wubin Zheng,Guanglei Lv,Zewei Quan,Chunxia Li
In recent years, rare earth doped upconversion nanocrystals have been widely used in different fields owing to their unique merits. Although rare earth chlorides and trifluoroacetates are commonly used precursors for the synthesis of nanocrystals, they have certain disadvantages. For example, rare earth chlorides are expensive and rare earth trifluoroacetates produce toxic gases during the reaction. To overcome these drawbacks, we use the less expensive rare earth hydroxide as a precursor to synthesize β-NaYF4nanoparticles with multiform shapes and sizes. Small-sized nanocrystals (15 nm) can be obtained by precisely controlling the synthesis conditions. Compared with the previous methods, the current method is more facile and has lower cost. In addition, the defects of the nanocrystal surface are reduced through constructing core–shell structures, resulting in enhanced upconversion luminescence intensity.
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SPECTROSCOPY LETTERS
·
2026
·
IF 1.4
Shijun Yu,Haiting Sun,Jiawei Huang,Jiali Gu
The effect of β-cyclodextrin (β-CD) coexistence and encapsulation on the interaction between butylated hydroxyanisole (BHA) and bovine serum albumin (BSA). The results of various spectral analyses, including fluorescence spectroscopy, three-dimensional (3D) fluorescence spectroscopy, Fourier transform infrared spectroscopy (FT-IR), and circular dichroism spectroscopy (CD), revealed that β-CD suppresses BHA-BSA binding through dual mechanisms, thereby mitigating BHA-induced conformational alterations in BSA. Specifically, upon formation of the BHA/β-CD inclusion complex, the binding affinity of BHA to BSA was significantly weakened, with the binding constant decreasing from 6.10 × 103 M−1 to 5.36 × 102 M−1. The thermodynamic analysis indicated that BHA to BSA binding is primarily driven by hydrophobic interactions. However, β-CD shifts this interaction to is a weakly entropy-driven or enthalpy-entropy compensation mechanism. Moreover, the coexistence and encapsulation of β-CD did not significantly affect BHA’s antioxidant capacity and esterase-like activity. The β-CD encapsulation protects BSA’s native conformation.
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JOURNAL OF CHROMATOGRAPHY A
·
2026
·
IF 4
Lin Lv,Hongmei Liu,Tao Song,Yan Zhao
Given the extensive use of amphenicol antibiotics and their potential adverse effects on human health, the development of simple and highly sensitive detection methods is essential to ensure food safety. In this work, a novel and highly efficient magnetic sorbent based on β-cyclodextrin-modified magnetic multi-walled carbon nanotubes functionalized with MIL-101(Cr) (Fe 3 O 4 @β-CD@MWCNTs@MIL-101(Cr)) was developed and combined with ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) to achieve highly sensitive detection of three amphenicol antibiotics (chloramphenicol, thiamphenicol, and florfenicol) in honey samples. The key factors influencing extraction efficiency in the magnetic solid-phase extraction process were identified using the Plackett-Burman experimental design, whereas the optimal experimental conditions were determined through Box-Behnken design-based response surface methodology optimization. Under these optimized conditions, favorable linear relationships for amphenicol antibiotics were observed within the concentration range of 0.5–250 ng mL − 1 (R 2 ≥ 0.9951). The method exhibited detection limits of 0.04–0.07 μg kg − 1 . Recovery rates ranged from 83.2% to 97.9%, with intra-day and inter-day precisions both below 7.2%. This method provides an efficient tool for monitoring amphenicol antibiotics in complex matrices.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Shunli Ji,Jinqiu Xu,Hong Shen,Xiaofei Ma
This study is the first to apply polymeric deep eutectic solvents (PDES) for enantioseparation in capillary electrophoresis (CE). We prepared two types of PDESs using poly(diallyldimethylammonium chloride) (PDDA) as the hydrogen bond acceptor and two polyhydroxy compounds as hydrogen bond donors. Due to the stable positive charges of this PDES, it can adsorb negatively charged chiral selectors (carboxymethyl-β-cyclodextrin, CM-β-CD) in the buffer solution, self-assembling to form composite nanoparticles. We characterized the PDES and composite nanoparticles using nuclear magnetic resonance, infrared spectroscopy, transmission electron microscopy, scanning electron microscopy, particle size analysis and the Tyndall effect. The nanoparticles provided a rich platform for chiral separation. We optimized several important separation parameters and successfully separated five chiral drugs. The results indicated that, compared to the single CM-β-CD system, the separation of model drugs was significantly improved in the composite nanoparticles system. Furthermore, we investigated the related chiral recognition mechanism using nuclear magnetic resonance method. The proposed composite nanoparticles exhibit a simple structure, is easy to prepare, and possesses superior separation performance. This research not only broadens the application of DES for enantioseparation in CE but also enriches the variety of chiral functionalized nanomaterials, taking a crucial step towards the development of new chiral recognition materials.
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