APOPTOSIS
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2026
·Vol. 31
·期 3
·78
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IF 9
Huang Xiaofeng,Shen Fei,Wang Dengshu,Wen Yaojuan,Zhang Yunqiang,Sheng Ning,Jiang Xuewei,Wu Song,Mi Yedong
Non-small cell lung cancer (NSCLC) metastasis, driven by tumor-associated macrophages (TAMs), remains a significant challenge due to poor prognosis and limited therapeutic options. This study developed DSPE-PEG-M2pep-modified liposomal nanoparticles (M2pep-LNP@siITGB4) delivering siRNA targeting integrin β4 (siITGB4) to reprogram M2 TAMs and induce apoptosis in NSCLC cells, thereby inhibiting metastasis. Cationic liposomes were prepared using thin-film hydration and ultrasonic emulsification, with M2pep peptides enhancing targeted delivery to M2 macrophages. In vitro, THP-1-derived M2 macrophages were co-cultured with A549 and NCI-H1299 cells, and the effects on macrophage polarization and tumor cell behavior were assessed via RT-qPCR, Western blot, and Transwell assays. In vivo, A549 xenograft and lung metastasis models were analyzed using IVIS, flow cytometry, and RNA sequencing. M2pep-LNP@siITGB4 downregulated M2 markers (CD206, Arg1, IL-10), upregulated M1 markers (CD86, iNOS), and increased CD8 + T cell infiltration. Silencing ITGB4 reduced GNB5 expression and FAK/Src/AKT phosphorylation, promoting apoptosis and inhibiting epithelial-mesenchymal transition (EMT). RNA-seq revealed 3494 differentially expressed genes, with suppressed ECM-receptor interactions. Tumor volumes and metastatic lesions were significantly reduced. This approach effectively reprograms TAMs, induces tumor cell apoptosis, and suppresses NSCLC metastasis, offering a novel nanomedicine-based strategy for enhancing anti-tumor immunity and improving therapeutic outcomes in NSCLC. Graphical abstract Schematic illustration of the mechanism by which M2pep-LNP@siITGB4 silences ITGB4 to modulate the integrin-FAK/Src-AKT signaling axis, reprogram macrophage polarization, and inhibit EMT (created with BioRender).
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Nature Communications
·
2026
·
IF 18.1
Cui Junfeng,Rao Yi,Gao Jianbao,Zhang Hao,Lin Cheng,Zhao Jiale,Zeng Jiawen,Fang Chun,Wang Zhiqiang,Wen Jinyu,Song Bo,Huang Yunhui,Yang Haiping,Xie Jia,Yao Yonggang
Sustainable batteries necessitate high-performance hard carbon negative electrodes derived from abundant biomass. However, realizing their full potential is significantly limited by the inherent diversity of biomass feedstocks, the intricate control over carbonization and resulting microstructures, and the complex interplay between processing, structure, and electrochemical performance. Here, we introduce “intelligent carbonization”, a strategy integrating programmable Joule heating (1000-2000 °C, 10-60 s) with machine learning to substantially accelerate the discovery and optimization of biomass-derived hard carbons. By mapping over 1000 synthetic pathways and decoding the multidimensional feature space, we reveal a performance-correlated factor that serves as a crucial predictor of capacity, complementing conventional graphitic descriptors (in-plane crystallite size/ interlayer spacing). By a minimal energy input (0.1 kWh g −1 ), our strategy converts biochar into advanced hard carbon delivering 369 mAh g −1 reversible capacity, high rate capability, and improved cycling stability (>5000 cycles at a specific current of 3 A g −1 ). This data-centric approach allows low-cost and intelligent manufacturing of diverse biomass resources into performance-unified hard carbon negative electrodes, thereby paving the way for practical and large-scale biomass valorization towards sustainable energy storage solutions.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2026
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IF 4.1
Li Xiang,Ling Ling,Chi Chaoxian,Ding Chuan-Fan
The development of a rapid analytical method for distinguishing chiral sulfur-containing compounds is essential due to the significant differences in biological activity exhibited by different enantiomers. This work presents a straightforward and rapid method for the chiral discrimination of D/L-homocysteine (D/L-Hcy), D/L-homocystine (D/L-Hcy 2 ), and S-allyl-D/L-cysteine (D/L-SAC) using cyclodextrin (CD) and metal adducts in conjunction with ion mobility-mass spectrometry (IM-MS). Through the formation of ternary complexes, separation was achieved with IM peak resolutions of 1.41 for [α-CD+D/L-Hcy+Co−H] + , 1.24 for [α-CD+D/L-Hcy 2 +Ca−H] + , and 1.65 for [β-CD+D/L-SAC+Fe−H] + . Theoretical calculations were performed to identify the optimal conformations of these ternary complexes. Conformational analysis elucidated distinct structural differences at the molecular level, providing insight into the chiral separation mechanism. Owing to its superior resolving power, this method enables the direct quantification of D/L enantiomers in mixtures. It was successfully applied to the determination of D/L-SAC in garlic samples, obviating the need for laborious sample pretreatment or derivatization steps. Overall, this work establishes a robust analytical platform for the chiral analysis of sulfur-containing amino acids and their derivatives. Graphical abstract
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Nature Communications
·
2026
·
IF 18.1
Luo Yaling,Chen Ruotian,Dittrich Thomas,Gao Pan,Ni Chenwei,Zhang Jie,Zhao Yue,Zhang Deyun,Ta Na,Li Mingrun,Zhang Mingjian,Li Dongfeng,Feng Zhendong,Li Zheng,Yu Yang,Zhou Panwang,Domen Kazunari,Fan Fengtao,Li Can
Photocatalytic overall water splitting remains limited by inefficient charge separation and utilization in reactions. Al-doped SrTiO 3 exhibiting near-100% apparent quantum efficiency for overall water splitting indicates nearly complete charge separation and surface catalytic efficiency. Although Al doping has been assumed to enhance charge separation and transfer, the exact role of Al is still unclear. Here, using spatiotemporal surface photovoltage imaging, we show that a gradient Al doping in Al-doped SrTiO 3 generates a built-in electric field that drives photogenerated holes from the bulk toward surface trap sites in the form of hydroxylated Al-O-Ti, prolonging their lifetime from ~100 ns to 10 ms. Spectroscopic analyses reveal that these hydroxylated Al sites serve as key centers for water adsorption, facilitating water oxidation. These findings underscore the pivotal role of Al in the spatiotemporal alignment of hole transfer and surface catalytic water oxidation, enabling high-efficiency photocatalysis in overall water splitting.
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Cell Death & Disease
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2026
·Vol. 17
·期 1
·226
·
IF 12.2
Rao Yongjuan,Wang Yunran,Gao Jie,Guan Xun,Lv Sixuan,Gu Junlian,Wang Kefang
Overactive bladder (OAB) is strongly linked to intravesical inflammatory responses. Transient receptor potential ankyrin-1 (TRPA1) is a key sensor and signaling molecule in bladder function, crucial for initiating and maintaining inflammation. However, the mechanisms underlying TRPA1-mediated inflammatory processes in OAB remain unclear. This study aims to elucidate the molecular mechanisms of TRPA1 and the contribution of inflammatory pathways to OAB. Elevated TRPA1 expression was observed in urinary sediment cells from OAB patients and in bladder tissues from OAB animal models. Mechanistically, TRPA1 drives the progression of OAB by activating the Nod-Like Receptor Family Pyrin Domain Containing 3 (NLRP3) inflammasome and triggering pyroptosis. Notably, treatment with HC-030031 effectively mitigated inflammatory responses and restored bladder function, whereas Nlrp3 overexpression negated these therapeutic benefits. Furthermore, TRPA1-mediated upregulation of NLRP3 was dependent on the transcription factors MAZ and SMAD3, highlighting a novel regulatory axis. Our findings establish TRPA1 as a pivotal mediator in the progression of OAB by activating the NLRP3 inflammasome and thereby inducing pyroptosis. Targeting TRPA1 or the NLRP3 signaling pathway represents a promising therapeutic strategy for OAB, offering new insights into disease management and potential improvements in patient outcomes.
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Journal of Ovarian Research
·
2026
·
IF 5.3
Li Xingying,Hu Xueyuan,Yang Jing,Yue Xiuyong,Deng Lilin,Xiong Qian
Background Bushen Huatan Formula (BSHT) shows promise in treating reproductive endocrine disorders, yet its mechanism in polycystic ovary syndrome (PCOS) remains unclear. This study aims to elucidate the specific mechanism of action of BSHT in treating PCOS. Methods PCOS model in rats was established by using a high-fat diet combined with letrozole gavage, and an in vitro model was constructed by stimulating human ovarian granulosa cells with dehydroepiandrosterone. Oestrus cycle changes were monitored throughout the BSHT intervention period via daily vaginal smear. Following intervention, ovarian histopathology and serum sex hormones, lipid profiles, oxidative stress, and ferroptosis markers were assessed. Network pharmacology predicted BSHT targets and pathways, validated in vitro/in vivo via TP53 pathway expression and downstream factors, oxidative stress, and key ferroptosis protein levels. Results High-dose BSHT effectively improved estrous cycle irregularities, reduced ovarian and uterine indexes, and repaired ovarian pathology in PCOS rats. BSHT treatment significantly improved endocrine profiles by reducing testosterone and luteinizing hormone levels while increasing follicle-stimulating hormone ( P < 0.001), and ameliorated dyslipidemia by lowering total cholesterol and low-density lipoprotein cholesterol while elevating high-density lipoprotein cholesterol ( P < 0.001). Mechanistically, BSHT suppressed oxidative stress and ferroptosis, as evidenced by decreased malondialdehyde, reactive oxygen species, lipid peroxides, Fe2 + and ACSL4, along with increased GSH and GPX4 ( P < 0.001). Both network pharmacology and experimental validation confirmed that these effects were mediated through inhibition of the TP53 signaling pathway and its downstream targets ( P < 0.001). The critical role of TP53 was further verified through intervention studies using ferroptosis inhibitors and TP53 agonists ( P < 0.001). Conclusion This study demonstrates that BSHT alleviates PCOS pathology by inhibiting ferroptosis and regulating lipid metabolism via the TP53 signaling pathway, providing a theoretical basis for novel therapeutic strategies.
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高影响因子
Nature Communications
·
2026
·
IF 18.1
Lu Xulei,Liu Jialin,Fu Chunqiao,Duan Peng,He Changliu,Wang Chenxing,Zhang Yong,Huang Yinpeng,He Qi-Chang,Yang Tingting
Moisture-induced ion diffusion in nanostructured materials is a promising route for high-performance power generation, yet achieving the continuous ion migration necessary for long-term operation remains challenging. Here, we report a moisture-electricity conversion mechanism capable of sustained electrical output. We demonstrate that humidity fluctuations regulate the dynamic coordination between iodide ions (I - ) and iodine molecules (I 2 ), driving the oscillatory migration of I - to generate a continuous alternating ion current (AC). Crucially, this coordination process avoids charge exchange, while electricity generation is achieved through moisture-driven ion transport. The device achieves a current output of 33.2 µA cm - ² and exhibits sustainable performance recovery under natural humidity fluctuations. This mechanism remains effective even under minimal humidity gradients (13% RH), ensuring adaptability to diverse weather conditions. Our strategy, applicable to various gel materials, utilizes environmental humidity fluctuations as a power source for ion diffusion, offering a fundamental solution for long-term, autonomous energy harvesting.
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JOURNAL OF MATERIALS RESEARCH
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2026
·
IF 2.9
Li Jie,Li Xiaojuan,Wang Xinran,Liu Xinyu,Wang Yingfeng,Wu Dingsheng,Feng Quan,Xu Zhenzhen,Chen Lei,Sheng Mingfei,Yin Maoli
Addressing limitations of conventional dressings in real-time monitoring and active repair, this study developed a smart, multifunctional nanofiber membrane by electrospinning curcumin (Cur) into a polyvinyl alcohol (PVA) matrix. The PVA/Cur membranes integrated antibacterial, antioxidant, and pH-responsive colorimetric properties. Increasing Cur content (0–1.5 wt%) reduced fiber diameter and enhanced hydrophilicity. The PVA/Cur1.0 membrane exhibited optimal mechanical properties (2.84 MPa tensile strength, 43.31% elongation). Biologically, PVA/Cur1.5 achieved a > 5-log reduction within 10 min and over 70% DPPH radical scavenging. Leveraging Cur's pH sensitivity, the dressing enabled visual wound monitoring via a distinct color transition from yellow (acidic, pH 4–6) to reddish-brown (alkaline, pH 7.5–8.9), synchronized with pH-triggered Cur release. Excellent biocompatibility was confirmed (hemolysis < 3%, fibroblast viability > 85%). This work presents an integrated strategy for a smart theragnostic dressing, merging real-time diagnosis and active therapy to advance beyond passive wound care. Graphical
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MOLECULAR AND CELLULAR BIOCHEMISTRY
·
2026
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IF 4.7
Du Jiangxia,Shen Minhui,Mu Yuan,Peng Shuoran,Xu Zhifei,Yang Xiaochun,Yang Bo,Luo Peihua,Yan Hao,He Qiaojun
Metabolic dysfunction-associated steatotic liver disease (MASLD), formerly known as non-alcoholic fatty liver disease (NAFLD), is a prevalent chronic liver disorder with complex pathogenesis and limited therapeutic options. Here, we identify the solute carrier SLC13A3 as a critical regulator of MASLD progression. In a mouse model fed a high-fat, high-cholesterol, and high-fructose (HFHCHF) diet, hepatic SLC13A3 expression was significantly upregulated and positively correlated with disease severity. Liver-specific overexpression of Slc13a3 exacerbated hepatic steatosis, lipid accumulation, and metabolic dysfunction, whereas Slc13a3 knockdown attenuated these pathological phenotypes. Targeted metabolomic analysis revealed that SLC13A3 modulates hepatic NAD + levels, thereby influencing the expression of key lipid metabolism genes, including SREBF1 , CD36 , PPARγ , and SCD1 . These findings highlight a previously unrecognized role of SLC13A3 in MASLD pathogenesis and suggest its potential as a therapeutic target.
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Nature Communications
·
2026
·
IF 15.7
Lu Gan,Hu-Rong Yao,Jingwen Dai,Zefeng Lai,Xin-Guang Yuan,Wei-Huan He,Qi-lin Zheng,Min Wen,Wei-Wei Yuan,Min Lin,Jian-Mao Xiao,Ze Yu,Yu-Jie Guo,Jinling Liu,Fanghua Ning,Fei Zhan,Denglong Chen,Lituo Zheng,Zhigao Huang,Chuying OuYang,Yu-Guo Guo
Exploring sodium-ion layered oxides with broad compositional diversity is an important approach for the development of high-performance positive electrodes. Structural chemistry determined by composition plays a decisive role in performance improvement, but the relationship between composition and structure becomes more elusive in complex multi-component systems. Here we propose an electronegativity entropy weight concept to understand entropy-dominated phases formation. Electronegativity and configurational entropy are used to quantify key interactions in layered materials. Guided by this understanding, we design a sodium-deficient layered oxide with an O3 stacking sequence. This material demonstrates good structural and thermal stability, along with air stability (negligible performance degradation after air exposure), cycling stability (93.02% capacity retention after 200 cycles), and rate capability (retaining 69.1% capacity retention from 86.5 mA g⁻¹ to 1.73 A g⁻¹). Even in potassium-ion batteries with larger inserted ions, the material still exhibits cycling stability. This strategy provides valuable compositional guidance for the rational design of high-performance layered oxide materials. The structure of layered oxides dictates their properties as positive electrodes in sodium-ion batteries, but its rational design remains challenging. Here, authors employ electronegativity and configurational entropy as a design descriptor to guide the synthesis of a sodium-deficient layered oxide with an O3 stacking sequence, rendering good thermal and air stability.
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WATER AIR AND SOIL POLLUTION
·
2026
·Vol. 237
·期 8
·497
·
IF 3.5
Yang Yuanfang,Xu Yunlan,Tang Danli,Xiang Tanxia
In this work, CoFe-layered double metal oxide (CoFe-LDO) was obtained by a co-precipitation-calcination route using CoFe-layered double metal hydroxide (CoFe-LDH) as precursor material, and then MoS 2 and chitosan-derived N-doped carbon co- modified CoFe-LDO composite catalyst (MoS 2 @CoFe-LDO/NCS) was prepared. This material was characterized by SEM, EDS, FTIR, XRD and XPS, and was synthesized to catalyze ozonation for the degradation of methylene blue (MB). The influence of CoFe-LDO dosage, O 3 flow rate, initial MB concentration, catalyst dosage, initial pH and coexisting compounds on MB removal efficiency were systematically investigated. The decomposition efficiency of 50 mg/L MB by 10%MoS 2 @CoFe-LDO/NCS reached 96.47% within 8 min under the conditions of 10% CoFe-LDO dosage (CoFe -LDO:CS), pH = 6.8, catalyst dosage of 0.4 g/L, Ozone flow rate of 1.87 mg/(L⋅min) and 25 °C. Stability tests and metal ion leaching experiments confirmed the excellent chemical stability and environmental compatibility of 10%MoS 2 @CoFe-LDO/NCS. Electrochemical characterization showed that 10%MoS 2 @CoFe-LDO/NCS possessed enhanced current density, redox activity and excellent electron transfer capability. Quenching experiments revealed that 1 O 2 , O 2 ∙ − and ∙ OH contributed to the MB degradation process, with O 2 ∙ − confirmed as the dominant reactive species.
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JOM
·
2026
·
IF 2.3
Yang Xue,Zhang Xin,Liu Aimin,Shi Zhongning
Magnesium-lithium alloys have emerged as promising lightweight materials for aerospace applications due to their outstanding specific strength and low density. In this study, an aluminum coating was electrodeposited onto a commercial LA103Z magnesium-lithium alloy using an environmentally friendly aluminum chloride-triethylamine hydrochloride (AlCl 3 -Et 3 NHCl) room-temperature ionic liquid electrolyte to enhance its corrosion resistance. The aluminum coating serves as a protective layer that improves the surface functionality and extends the service life of the magnesium-lithium alloy. The electrodeposition mechanism, surface morphology, microstructure, and corrosion behavior of the aluminum coatings were systematically investigated using scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, and electrochemical analysis. The results reveal that aluminum electrodeposition in the AlCl 3 -Et 3 NHCl electrolyte follows a three-dimensional instantaneous nucleation mechanism, with the reduction of electroactive Al 2 Cl 7 − ions to metallic aluminum being diffusion-controlled. At a deposition potential of − 0.4 V, the resulting aluminum coating exhibited a smooth, compact, and uniform morphology. However, as the deposition potential shifted to more negative values, the coating surface became rougher, accompanied by crack formation and increased grain size. Furthermore, higher deposition temperatures led to the development of coarser and less uniform grains. The optimal coating performance was achieved at a deposition potential of − 0.4 V and a temperature of 303 K, where the aluminum layer displayed fine grains, low surface roughness, and superior corrosion resistance.
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