ARCHIVES OF DERMATOLOGICAL RESEARCH
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2025
·Vol. 317
·期 1
·942
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IF 2.1
Yang Mingmei,Wang Huiying,Huang Zeyu,Zhang Ruzhi
Mesenchymal stem cell-derived exosomes (MSC-Exos) have been shown to protect melanocytes from oxidative injury, suggesting their potential contribution to therapeutic strategies for vitiligo. Multilineage-differentiating stress-enduring (Muse) cells are a unique subpopulation of mesenchymal stem cells (MSCs) that can tolerate stress and differentiate into multiple cell types. However, the effects of Muse cell-derived exosomes (Muse-Exos) on melanocyte injury are not well understood. In this study, we investigated whether Muse-Exos could protect human epidermal melanocytes (HEMs) from hydrogen peroxide (H 2 O 2 )-induced oxidative stress injury in vitro. Muse cells were isolated from MSCs via magnetic-activated cell sorting (MACS), and the exosomes were collected by ultracentrifugation. The exosomes were characterized using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and Western blotting. HEMs were pretreated with either MSC-Exos or Muse-Exos at concentrations of 5, 10–20 µg/mL for 48 h, after which they were exposed to H 2 O 2 to induce oxidative stress. Functional assays were conducted to assess cell viability, apoptosis, reactive oxygen species (ROS) accumulation and autophagy-related markers (LC3B-II, Beclin-1 and p62). Both MSC-Exos and Muse-Exos reduced H 2 O 2 -induced cytotoxicity and promoted cell survival. Muse-Exos were found to have relatively stronger effects in reducing ROS levels, inhibiting apoptosis, and enhancing autophagic activity. These results suggest that Muse-Exos may protect melanocytes against oxidative stress in vitro and could inform new approaches to treating vitiligo using exosomes.
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JOURNAL OF NANOBIOTECHNOLOGY
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2025
·
IF 12.6
Hu Jiayao,Liu Hao,Wu Yizhou,Dong Xiaoyan,Tang Dongying,Yan Wei,Liu Bin,Zhou Hongyan
The active ingredients of Traditional Chinese Medicine with diverse structures exhibited anti-inflammatory and lipid lowering functions, demonstrating significant therapeutic effects in inflammatory diseases of atherosclerosis. We incorporate Astaxanthin (AST) and Dihydroartemisinin (DHA) into PLGA NPs to synthesized HA@PLGA@AST/DHA NPs (HPAD NPs) for alleviating atherosclerosis. In vitro assay indicated that the designed HPAD NPs promoted cholesterol efflux of macrophages by enhancing selective lipophagy, which is benefit to lipid antigen degradation. Meanwhile, HPAD NPs regulated T-cell differentiation and crucially induced macrophages from pro-inflammatory M1 type to anti-inflammatory M2 type. In vivo study demonstrated that HPAD NPs decreased the necrotic core dimension and improved plaque stability in ApoE -/- mice with atherosclerosis. Overall, this research indicated the promise of HPAD NPs for the targeted therapy of atherosclerosis. Graphical abstract Schematic illustration of HPAD NPs preparation and therapeutic strategy for AS. (A) Preparation of HPAD NPs. (B) HPAD NPs reduce inflammation and prevent AS by restoring lipophagy of macrophages and promote the polarization from M1 type to M2 type to modulate the AIM.
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Communications Chemistry
·
2025
·Vol. 8
·期 1
·389
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IF 6.2
Han Lei,Tan Jingying,Zhang Yucui
Given the promising prospect of nanozymes in colorimetric test strips, it is essential to eliminate the interferences of their multi-activities and various colors on the test strip. Here, white Mn-based metal-organic frameworks (Mn-MOFs) with ultrathin 2D morphology (3 nm thick) were successfully synthesized by a simple ultrasonic approach. The origin of the white optical property in Mn-MOFs was systematically investigated, revealing that it stems from specific metal-ligand coordination polymerization rather than morphological features or defect states. Mn-MOF nanozymes possessed exclusive peroxidase-mimicking activity rather than oxidase-like activity, effectively resisting O 2 interference during colorimetric assay. Moreover, Mn-MOF nanozymes displayed unique substrate selectivity without additional modification. Unlike other colored nanozymes, the whiteness of Mn-MOF nanozymes enhanced the paper’s whiteness, boosting contrast for colorimetric detection on test strip. This study pioneers a systematic investigation into the origin of whiteness in MOF nanozymes. The coordination-defined properties enable interference-free optical design and O 2 -resistant on-site detection.
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Nature Communications
·
2025
·Vol. 16
·期 1
·10834
·
IF 15.7
He Lin,Li Menggang,Qiu Longyu,Ren Xue,Tian Fenyang,Sheng Jie,Liu Yequn,Luo Mingchuan,Zhou Xin,Yu Yongsheng,Yang Weiwei,Guo Shaojun
A largely unexplored approach for optimizing surface strains on terrace-type catalysts is the break of atomic symmetry to release surface stress. The key challenge lies in how to implement this approach into practical nanocatalysts, in particular the promising high-entropy alloys (HEAs). Herein, we design and synthesize a series of HEA nanorings (NRs) with abundant terrace-type defects for oxygen reduction reaction (ORR) electrocatalysis. The asymmetry-triggered release of surface stress enables the modulation of compressive strain for optimizing the electronic structure. On the optimally-tuned PtPdFeCoNi HEA NRs, we achieve mass and specific activities of 0.99 A mg -1 platinum group metal (PGM) and 1.32 mA cm -2 PGM at 0.95 V versus reversible hydrogen electrode (vs. RHE), demonstrating a competitive performance. Experimental and theoretical investigations unveil that the stress-released compressive strain lowers the d -band center of Pt sites in HEA NRs, resulting in favorable desorption of oxygenated intermediates and thus accelerated ORR kinetics.
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3 Biotech
·
2025
·Vol. 15
·期 12
·412
·
IF 2.9
Fang Zhiruo,Zhu Haojun,Gao Jiancao,Cao Liping,Yao Yu,Li Quanjie,Dai Fei,Yang Xiwei,Du Jinliang,Xu Gangchun
Silymarin has proven antioxidant activity and hepatoprotective effects, but no study to date has investigated the potential protective effect on deltamethrin-induced hepatopancreas injury in Chinese mitten crabs Eriocheir sinensis . This study investigated the protective effect of silymarin pretreatment on deltamethrin-induced hepatopancreas injury in E.sinensis . Healthy crabs (n = 150) were randomly divided into five experimental groups: the control, a deltamethrin exposure group (Basal feed without silymarin), and three silymarin groups (addition of 0.1, 0.5, or 1.0 g/kg diet to the basal feed). After 6 weeks of feeding (no deltamethrin exposure), the crabs were exposed to a deltamethrin concentration of 4.317 μg/L for 48 h. Histopathological results showed that the hepatopancreas remained relatively intact in the silymarin pretreatment groups. Furthermore, metabolites annotated by LC–MS were mainly enriched in signaling pathways related to cytochrome P450, terpenoid alkaloid biosynthesis and phospholipid metabolism. Silymarin at 0.1, 1.0 and/or 0.5 g/kg were significantly increased AKP, ACP, GSH activities and T-AOC levels, and significantly decreased AST, ALT, TNF-α, IL-6, NO, TC, MDA, CYP3A and CYP2E1 levels ( P < 0.05). Silymarin at the concentation of 0.1, 0.5 and 1.0 g/kg could significantly up-regulated the expressions of immune-related genes ( propo , alf3 , crustin1 , hsp70 , hsp90 , relish ), anti-apoptotic gene ( bcl-2 ), and significantly down-regulated the lipid metabolism-related genes ( cpt1 , srebp , acc ) and apoptosis-related genes ( caspase3 , caspase8 , p53 , cyt-c ) ( P < 0.05). Silymarin at 0.5 and/or 1.0 g/kg significantly down-regulated the mRNA expression of inflammation-related genes ( tnf-α , il-16 ) and drug metabolism-related genes ( cyp2a , cyp2e1 , cyp3a ) ( P < 0.05). The results demonstrate a good protective effect of 1.0 g/kg silymarin on deltamethrin-induced hepatopancreas injury induced by deltamethrin in a crustacean species.
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Journal of Ocean University of China
·
2025
·Vol. 24
·期 6
·1827-1833
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IF 1.2
Xin Wenyuan,Zhou Yunbing,Liu Daixi,Wang Cong,Sherwin K. B. Sy
Astaxanthin (AX) and vitamin E (VE) are widely consumed nutritional supplements in China, with its beneficial effects predominantly attributed to all-trans AX and VE. The aim of this study is to develop and validate a rapid and accurate method for quantifying the content of AX and VE in nutritional supplement products using highly sensitive 1 H NMR method. Coumarin was chosen as the internal standard. Specific signals from AX was optimal at H-7,7′ in the chemical shift range of δ 6.17–6.24 ppm, whereas the signals of VE at δ 2.59 ppm. To demonstrate the reliability of this analytical approach the proposed method underwent rigorous validation, specificity, limit of detection (LOD), limit of quantitation (LOQ), linearity, accuracy, precision, and recovery. The accuracy of the validation method was 3.10% for AX and 1.99% for VE. The results indicated that the method was precise and reliable. The method has been successfully applied to simultaneous quantification of AX and VE in nutritional supplements products.
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-11
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IF 3.9
Xie Xuwen,Zhang Siyu,Zhao Zichen,Li Shulei,Niu Qing,Wu Chen,Liu Xin,Wu Yan,Zhao Xing
Polyvinyl butyral (PVB) is commonly used as an adhesive for cultural relics. However, PVB suffers from poor stability, yellowing, and decreased adhesion performance after aging. Here, graphene quantum dots (GQDs) were used as stabiliser materials to modify PVB and obtain GQDs@PVB that was improved in anti-UV/thermal aging. The yellowness index, adhesive strength, chemical structure, and thermal properties of PVB and GQDs@PVB, before and after aging, are characterized using a benchtop spectrophotometer, universal testing machine, Fourier-transform infrared spectrometer, and simultaneous thermal analyzer. The results indicate that during the aging process, GQDs@PVB shows a lower yellowness index and higher adhesive strength than that of PVB consistently. Meanwhile, it exhibits a lower intensity of carbonyl peaks generated by thermal aging with a thermal decomposition temperature increased by 19 °C. The addition of GQDs improves the photothermal stability of PVB and holds promise for the stability research of materials in relics conservation.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 12
·846
·
IF 5.3
Guan Yafei,Li Ying,Zheng Yan,Zhou Dongshan,Xia Zhengkun
Acute kidney injury can cause changes in fluid glucose levels, such as blood, sweat, or tears. However, the skin of vulnerable populations, such as premature infants, is extremely fragile and cannot withstand frequent needle-based blood sampling. Detecting glucose in skin sweat provides a non-invasive new approach. This study utilizes laser-induced graphitization technology and secondary modification of electrodes to efficiently fabricate an enzymatic wearable glucose sensor patch on a flexible substrate on a large scale and at a low cost. Experiments show that the sensor exhibits good sensitivity (1.71 µA/mM) within a glucose concentration range < 10 mM. Moreover, it is not affected by other interfering substances in sweat (potassium chloride, sodium chloride, urea, ascorbic acid, etc.). Additionally, by integrating a smart adhesive with switchable adhesion on the patch surface, strong adhesion at body temperature (35 °C, 82.25 N/m) and on-demand removal under mild cooling were achieved (20 °C, 10.13 N/m), effectively avoiding potential skin damage caused by device detachment. This work provides new insights into the efficient and low-cost fabrication of comfortable wearable glucose sweat sensors. Graphical
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JOURNAL OF MATERIALS SCIENCE
·
2025
·
IF 3.9
Zhang Mengruo,Zhang Yuwei,Sun Jia,Feng Wenkai,Li Rao
Traditional wound dressings often fail to address the dynamic mechanical and biochemical demands of complex wound microenvironments, necessitating advanced materials with multifunctional adaptability. Herein, a multifunctional composite hydrogel (PCTM) was developed by integrating polyvinyl alcohol, carboxymethyl chitosan, tannic acid, and montmorillonite (MMT) through a synergistic physical crosslinking strategy. The freeze–thaw process, combined with hydrogen bonding and electrostatic interactions, enhanced anti-swelling capacity (equilibrium ratio: 1.11 in PBS). MMT reinforcement significantly improved mechanical robustness, achieving a tensile strength of 994.2 kPa, compressive strength of 461.6 kPa, and energy dissipation of 15.4 kJ/m 3 , ensuring structural stability under repetitive deformations. Dynamic reversible interactions endowed rapid self-healing (75.9% strength recovery) and strong tissue adhesion (26.2 kPa), critical for mechanically active wound regions. The hydrogel prepared in this work shows promising potential for biomedical applications, particularly as a skin wound dressing. Graphical abstract
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Nature Communications
·
2025
·Vol. 16
·期 1
·10322
·
IF 15.7
Li Xuan,Jiang Xiao,He Ye,Xiong Hongwei,Huang Qian,Xu Kun,Luo Xin-Xin,Li Kai,Tao Bailong,Zha Kangkang,Wu Jing,Liu Peng,Cai Kaiyong
The elimination of senescent cells can enhance the osteointegration of implants in elderly patients. However, achieving specific clearance of senescent cells without adversely affecting the function of normal cells remains challenging. Here we show an implant surface modification technique to achieve specific clearance of locally senescent cells by modulating their metabolism. Our method involve modifying implants with BPTES, a glutaminase 1 (GLS1) inhibitor, through π-π stacking with dopamine. This modification effectively induces intracellular acidosis in senescent mesenchymal stem cells (MSCs) through suppression of glutaminolysis. Simultaneously, poly(γ-glutamate) (PGA), modified by a layer-by-layer method, serve as a high-density carbon source coating, continuously supporting glutamine metabolism in MSCs without ammonia production. Our results show that modified implants significantly reduce the senescence level around implants and promote osteointegration in aged rats. These findings provide promising insights into the design and application of orthopedic implants for elderly patients.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·
IF 12.6
Shijin Xu,Naidi Yang,Fangning Du,Ziying Zhang,Yin Zhang,Yixuan Zhang,Jiawei Liang,Yihan Zhao,Jiajun Zhang,Ziwei Zhang,Xiaoxuan Han,Zena Chen,Zhiqiang Zhou,Shu Zhang,Lin Li,Ying Lv
Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies, marked by extensive stromal fibrosis and a profoundly immunosuppressive, immune-excluded tumor microenvironment (TME) that hampers the efficacy of immune checkpoint blockade. Although photodynamic therapy (PDT) can induce immunogenic cell death (ICD) and stimulate anti-tumor immunity, its effectiveness against PDAC is limited by insufficient immune activation and persistent stromal-mediated immunosuppression. To address these challenges, we develop a liposomal nanodrug that co-encapsulates a mitochondrial-targeted photosensitizer (MP) and a TGF-β receptor inhibitor (LY2109761) to synergize PDT with PD-1 checkpoint blockade. MP selectively accumulates in mitochondria and, upon light activation, amplifies mitochondrial reactive oxygen species production, inducing mitochondrial damage. This damage triggers the release of mitochondrial DNA and damage-associated molecular patterns, activating the STING pathway and eliciting potent ICD and anti-tumor immune responses. Simultaneously, liposome-mediated delivery of LY2109761 mitigates stromal desmoplasia and reverses TGF-β-driven immune suppression, enhancing effector T cell infiltration and activity. In murine PDAC models, this dual-action strategy transforms the immune-cold TME into an immune-inflamed phenotype, sensitizing tumors to PD-1 therapy and leading to pronounced tumor regression and prolonged survival. Our findings present a promising nanodrug-based approach to remodel the fibrotic and immunosuppressive TME of PDAC and enhance immunotherapeutic outcomes.
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2025
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IF 3.1
Yu Ziming,Han Zhiyue,Zhang Xinrui,Li Zerui,Tong Wenchao,Ding Jianxu
This study investigates the explosion suppression mechanism of a novel composite deflagration inhibitor (SK), synthesized from industrial waste blast furnace slag (S105) and KH 2 PO 4 , for the suppression of aluminum dust explosions. By employing a liquid-phase chemical coating method, SK integrates S105 (a low-cost, high-strength matrix) and KH 2 PO 4 (a chemically active carrier) into a core–shell structure, achieving synergistic suppression effects. Explosion suppression effect were evaluated using a dual-channel 20 L spherical explosion vessel and a Hartmann tube, revealing that SK reduced the maximum explosion pressure by 23.9%, the pressure rise rate by 70.7%, and the flame propagation speed by 67.3%. These results indicate that SK outperforms individual components (S105 and KH 2 PO 4 ) as well as conventional inhibitors (e.g., NaHCO 3 ). Thermogravimetry–differential scanning calorimetry (TG–DSC) and microstructure characterization techniques, such as scanning electron microscopy (SEM) and X-ray diffraction (XRD), elucidate the dual inhibition mechanisms: (1) Physical inhibition, through endothermic decomposition and release of H 2 O to dilute oxygen and form a physical barrier; (2) Chemical inhibition, which suppresses explosions by scavenging radicals and terminating chain reactions. S105 is more cost-effective than its components, primarily due to the ultra-low cost of slag. This work not only promotes the sustainable use of industrial waste resources but also provides a scalable and efficient solution for industrial explosion safety, aligning with global circular economy goals.
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