ACTA PHARMACOLOGICA SINICA
·
2019
·Vol. 40
·期 8
·1106-1118
·
IF 4.01
Zhao Kai-jing,Chen Yang,Hong Shi-jin,Yang Yi-ting,Xu Jiong,Yang Han-yu,Zhu Liang,Liu Ming,Xie Qiu-shi,Tang Xian-ge,Yang Ting-ting,Zhou Ya-qian,Liu Li,Liu Xiao-dong
Cinnamic acid and its analogues (pyragrel and ozagrel) undergo chain-shortened (β-oxidative) and reductive metabolism on acyl side chain. In this study, we characterized the β-oxidative and reductive metabolism on acyl side chain of cinnamic acid and its analogues using primary rat hepatocytes, hepatic mitochondrial, and microsomal systems. A compartmental model including parent compounds and metabolites was developed to characterize in vivo β-oxidative and reductive metabolism following an intravenous dose of parent compounds to rats. The fitted total in vivo clearance values were further compared with the in vitro values predicted by the well-stirred model. We showed that hepatic microsomal CYP450s did not catalyze β-oxidative or reductive metabolism of the three compounds. Similar to β-oxidation of fatty acids, β-oxidative metabolism on their acyl side chain occurred mainly in mitochondria, which was highly dependent on ATP, CoA and NAD + . Fatty acids and NADH inhibited the β-oxidative metabolism. Reductive metabolism occurred in both mitochondria and microsomes. Reduction in mitochondria was ATP-, CoA-, and NAD(P)H-dependent and reversible, which was suppressed by enoyl reductase inhibitor triclosan. Reduction in microsomes was ATP-, CoA-, and NADPH-dependent but little affected by triclosan. Both plasma concentrations of β-oxidative metabolites and reductive metabolites were successfully fitted using the compartmental model. The estimated total in vivo clearance values were consistent with those predicted from hepatocytes and organelles, implicating significance of in vitro kinetics. These findings demonstrate the roles of hepatic mitochondria and microsomes in β-oxidative and reductive metabolism on acyl side chain of cinnamic acid and its analogues along with their metabolic characteristics.
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JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
·
2019
·Vol. 34
·期 1
·145-149
·
IF 0.605
Wu Junqing,Guo Le,Wen Min,Bu Tongle,Zhou Peng,Zhong Jie,Huang Fuzhi,Zhang Qi
Long (15 - 40 μm), thin (diameter of 20 ± 5 nm), and well-dispersed CuNWs Cu nanowires were prepared. The high-resolution TEM and selected area electron diffraction showed that the CuNWs were single-crystalline. To investigate the growth mechanism, we examined the microstructure of these CuNWs at different reaction time. It was found that the CuNWs were actually formed through the self-assembling of Cu nanoparticles along the [110] direction. The transparent electrodes fabricated using the CuNWs achieved a high transparency of 76 % at 31±5 Ω/□.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-10
·
IF 5.479
Zhang Kaina,Hu Dehua,Deng Suimin,Han Meng,Wang Xiangfeng,Liu Hailing,Liu Yuan,Xie Mengxia
A novel magnetic nanomaterial for use in metal ion based affinity chromatography is described. It is based on the chelation between the phosphate groups of phytic acid (PA) and Ti(IV) ions. Due to the large number (6) of phosphate groups of PA, it has a large capacity for Ti(IV) ions. PA was first immobilized on magnetite nanoparticles (PA-MNPs) and then loaded with Ti(IV) ions to obtain the sorbent (Ti-PA-MNPs). The fraction of Ti(IV) ions on the surface of PA-MNPs that is exposed to the solution binds the phosphate groups of phosphopeptides. The bound phosphopeptides can then be magnetically separated. The method was applied to the enrichment of the phosphopeptides in a β-casein tryptic digest. A tryptic digest of bovine serum albumin (BSA) was added at a molar ratio (β-casein to BSA) of 1:2000 to study selectivity. The phosphopeptides were quantified by mass spectrometry. The limit of detection can be as low as 8 × 10 −10 mol L −1 . This sorbent has a high absorption capacity (53.5 μg mg -1 ) and shows good recoveries (90%). As many as 2145 phosphopeptides were isolated from 500 μg tryptic digest of a rat liver lysate after enrichment by Ti-PA-MNPs. This is superior to that (1568 phosphopeptides) of commercial TiO 2 kit.
Schematic presentation of fabrication for a novel modified magnetic nanomaterial (Ti-PA-MNPs) based on the chelation of phytic acid (PA) with Ti(IV) ions. Ti-PA-MNPs were successfully applied to enriching low abundance phosphopeptides from biosamples in mass spectrometric analysis.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2019
·Vol. 35
·期 1
·21-25
·
IF 1.071
Jiang Ling,Ye Wenting,Su Weike,Yu Chuanming
A new method for the synthesis of 1,4-dihydropyridine(1,4-DHP) calcium channel antagonists felodipine, nitrendipine and their derivatives via papain-catalyzed three-component reactions of aldehyde, methyl acetoacetate and ethyl 3-aminocrotonate was developed. Operational simplicity, mild reaction conditions and eco-friendliness are the key features of this protocol.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 26
·期 16
·16482-16492
·
IF 2.914
Tong Dongshen,Fang Kai,Yang Haiyan,Wang Jie,Zhou Chunhui,Yu Weihua
Sodium alginate (SA) is a linear biopolymer, which is the nontoxic, biodegradable, and rich in carboxyl and hydroxyl groups. In the paper, the SA-based hydrogel bead was prepared by the cationic hectorite clay and anionic sodium alginate with a simple ionic gelation method under freeze-drying, and the adsorption properties were evaluated by the removal of copper ions from aqueous solutions. The composites were characterized by X-ray diffraction (XRD), nitrogen adsorption-desorption isotherm (BET), thermal analysis (TG), and Fourier transform infrared spectroscopy (FT-IR). The pseudo-first-order and pseudo-second-order kinetic models were used to describe the kinetic data and the Langmuir, Freundlich, Dubinin–Radushkevich (D-R), and Temkin models were applied to describe the adsorption isotherms. The results showed that the adsorption process was found to follow the Freundlich isotherm model and the maximum sorption capacity was observed to be 160.28 mg/g under the initial concentration from 10 to 700 mg/L at 45 °C. Adsorption kinetics data fitted well with pseudo-second-order rate model. The porous structure of the composite was responsible for the adsorption of Cu 2+ ions. But the adsorption ability could be improved by pH. Finally, the adsorption mechanism was suggested.
Graphical abstract
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 5
·1-7
·
IF 5.479
Ma Shuyi,Wang Jinjie,Yang Guang,Yang Jingxia,Ding Derun,Zhang Min
A method is described for the preparation of copper(II)-modified keratin-capped gold nanoclusters (AuNCs) with adjustable Au/Cu molar ratio through a two-step synthetic route. The introduction of Cu(II) is known to cause quenching of the fluorescence of such AuNCs. It is found, however, that the Cu(II) loaded AuNC (AuNC-Cu 2+ ) display strongly enhanced peroxidase-like activity and improved chemical stability. This is assumed to be due to the synergistic effect of the gold and copper atoms and in contrast to the single components (pure AuNCs and copper ions). The kinetic parameters of the new peroxidase mimic show a higher K cat value (12.1 × 10 −4 s −1 ) and a lower K m value (53 μM) for H 2 O 2 (compared to those of conventional AuNCs). The catalytic activity is stable and remains essentially unchanged after two months. The interactions of AuNCs with Cu(II) were characterized by fluorescence spectroscopy, UV-vis spectroscopy and X-ray photoelectron spectroscopy. Based on these findings, a glucose colorimetric assay at 452 nm was developed that has a detection range from 1.6 to 800 μM and a 0.26 μM detection limit.
Copper ion-modified keratin-capped gold nanoclusters (AuNC-Cu 2+ ) exhibit enhanced peroxidase-like activity owing to the synergistic effect of the gold and copper atoms which is in contrast to pure AuNCs.
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Frontiers of Environmental Science & Engineering
·
2019
·Vol. 13
·期 2
·1-12
·
IF 3.883
Deng Yuzhou,Peng Shengpan,Liu Haidi,Li Shuangde,Chen Yunfa
Mesoporous TiO 2 was synthesized via nonhydrolytic template-mediated sol-gel route. Catalytic degradation performance upon dichloromethane over as-prepared mesoporous TiO 2 , pure anatase and rutile were investigated respectively. Disproportionation took place over as-made mesoporous TiO 2 and pure anatase under the presence of water. The mechanism of disproportionation was studied by in situ FTIR. The interaction between chloromethoxy species and bridge coordinated methylenes was the key step of disproportionation. Formate species and methoxy groups would be formed and further turned into carbon monoxide and methyl chloride. Anatase (001) played an important role for disproportionation in that water could be dissociated into surface hydroxyl groups on such structure. As a result, the consumed hydroxyl groups would be replenished. In addition, there was another competitive oxidation route governed by free hydroxyl radicals. In this route, chloromethoxy groups would be oxidized into formate species by hydroxyl radicals transfering from the surface of TiO 2 . The latter route would be more favorable at higher temperature.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2019
·Vol. 30
·期 10
·9259-9265
·
IF 2.195
Zhou Yujiu,Zhao Yuetao,Chen Fujia,Chen Yan,Liu Qingxia,He Xin,Mao Xiling,Yang Yajie,Xu Jianhua
The improvement of energy density is desired for dielectric materials in capacitors according to the developing trend of electric devices. In this article, poly(vinylidene fluoride-co-chlorotrifluoroethylene) (P(VDF-CTFE)) and polyurea (PUA) are blended in different ratios to obtain high dielectric performance composite, and the dielectric polymer composite films are fabricated and investigated. The composite films combine the advantages of both contents, showing high dielectric constant of P(VDF-CTFE) and high energy storage efficiency of PUA. The results demonstrate that the composite film contains PUA with a volume fraction of 10% exhibits the best performance, achieving a breakdown strength as high as 5020 kV/cm, which is higher than that of P(VDF-CTFE)/polythiourea (PTU) composite ever reported. Moreover, the breakdown strength and energy storage efficiency are 1.35 times and 1.65 times higher than pure P(VDF-CTFE), respectively. The article provides a new high-performance dielectric material fabricated through a simple and effective blending method, and it has a great potential in electric energy storage capacitors.
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CELLULOSE
·
2019
·Vol. 26
·期 5
·3095-3109
·
IF 3.917
Wang Weiku,Li Yinhui,Li Weijie,Zhang Baohua,Liu Yaodong
Abstract This study systematically investigated the structural changes of cotton-based microcrystalline cellulose (MCC) during solvent treatment in water and dimethylacetamide (DMAc), as well as how solvent exchange affects the dissolution rates of MCC in LiCl/DMAc solution. When MCC is soaked in DMAc or water for 3 days or longer, the surface cellulose layers become exfoliated from the MCC particles and these exfoliated portions are solvable in LiCl/DMAc. DMAc treatment significantly improves the nano-porosity of MCC. Thus, the chloride anions are able to diffuse into cellulose and lead to the final dissolution of cellulose chains via charge repulsion effects. On the other hand, water treatment exhibits strong pore closing effect, especially for nano-pores with a diameter < 30 nm, which prohibits the diffusion of chloride anions and the final dissolution of cellulose chains.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2019
·Vol. 21
·期 2
·1-13
·
IF 2.009
Li Fang,Huang Haibao,Li Guisheng,Leung Dennis Y. C.
Highly ordered TiO 2 nanotube arrays (TiO 2 NTs) were synthesized by anodization method using a titanium foil and further modified with nanoparticles ( Ø = 2~10 nm) of three platinum group metals (that is, platinum, palladium, and ruthenium) through potentiostatic pulsed electrodeposition method to obtain the composite material. Compared with pure TiO 2 NTs, all the three composite samples (M-TiO 2 NTs, M = Pt, Pd, Ru) showed different enhancement effects on the light responses, as well as different photoelectrochemical performances. In this study, the performance of M-TiO 2 NTs, which worked as photoanode and cathode, was investigated. Ru-TiO 2 exhibited the best degradation yield (~ 85.8%) when applied as photoanode under visible light illumination, which indicated the platinum group metal could also be induced under visible irradiation, not just served as the co-catalyst. M-TiO 2 NTs as cathode were evaluated under the hydrogen evolution reaction (HER). The three M-TiO 2 NT electrodes showed an improved efficiency over pure TiO 2 NTs, while Pt-TiO 2 NTs performed even better (without any sacrificial agent) with higher Faradic efficiency than platinum electrode in the photoelectrocatalytic hydrogen production, which could be explained by the uniform and fine metal nanoparticles on the surface of TiO 2 NTs to offer abundant active sites for the reaction.
In this paper, TiO2 nanotube arrays loaded with nanoparticles of platinum group metals have been explored on their enhancement of photoelectrocatalytic activity. Platinum group metals served as co-catalyst in the surface of TiO2 nanotubes and show great variations in different reactions.
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JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2019
·Vol. 319
·期 3
·1119-1125
·
IF 1.186
Wang Yunhan,Song Hu,Wang Guanquan,Yang Xia,Wang Jing,Wei Hongyuan
In this study, we successfully synthesized polyethylene glycol (PEG) and folic acid (FA) co-functionalized graphene quantum dots (GQDs) to improve the biocompatibility and tumor-targeting ability of GQDs simultaneously, and labeled GQDs–PEG–FA with 131 I for biological behavior evaluation. The in vitro properties, biodistribution and SPECT imaging of 131 I-GQDs–PEG–FA were investigated. The uptake of 131 I-GQDs–PEG–FA at tumor sites can be clearly examined via SPECT imaging, which can ascribe to enhanced permeability and retention effect and active targeting effect of FA to folate receptors. The results indicate that 131 I-GQDs–PEG–FA can be used as a radioactive probe for detection of tumor cells overexpressing folate receptor.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-9
·
IF 5.479
Zhang Zhi,Xu Guoli,Xie Li,Guan Yueping
A colorimetric immunoassay is described for the pregnancy marker human chorionic gonadotropin (HCG). The assay is based on the use of MnO 2 nanorods acting as a peroxidase mimic. The nanorods were prepared by a hydrothermal method using EDTA as a template. They exhibit excellent peroxidase-like activity and stability. The nanorods were immobilized in a chitosan matrix in the wells of a BSA-modified microplate which then were further modified with streptavidin and biotinylated capture antibodies. The specific recognition between HCG and the antibodies in wells inhibit the peroxidase-like activity of the nanorods. Hence, the substrate 3,3′,5,5′-tetramethylbenzidine is less efficiently catalytically oxidized by H 2 O 2 to form a blue colored product. This results in a decrease in the absorbance at 652 nm. Response is linear in the 0.5 to 400 mIU·mL −1 HCG concentration range, and the detection limit is 0.36 mIU·mL −1 under optimized conditions. The method is highly specific, acceptably reproducible and stable. It was applied to the determination of HCG concentration in serum samples, and results were in good agreement with data obtained by the reference method.”
Schematic presentation of colorimetric immunoassay for human chorionic gonadotropin (HCG) by using MnO 2 nanorods with peroxidase-like activity immobilized in microplate wells. The specific recognition between HCG and the antibodies in wells inhibits the peroxidase-like activity of the nanorods. TMB: 3,3′,5,5′-tetramethylbenzidine.
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