ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 29
·期 4
·6237-6250
·
IF 4.223
Chen Xi,Lin Hai,Dong Yingbo,Li Bing,Yin Tingting,Liu Chenjing
The complex mixtures of antibiotics and heavy metals are commonly existed in livestock and poultry breeding wastewater. Effective and simultaneous removal of these toxic compounds by microorganisms, especially single strains, remains a considerable challenge. In this study, a novel functional strain SDB4, isolated from duck manure and identified as Bacillus sp., has been shown to possess high removal capabilities for both sulfamethoxazole (SMX) and Zn 2+ . The maximum removal efficiency achieved 73.97% for SMX and 84.06% for Zn 2+ within 48 h in the single pollution system. It has great potential for eliminating SMX along with Zn 2+ , 78.45% of SMX and 52.91% of Zn 2+ were removed in the 20 mg·L -1 SMX and 100 mg·L -1 Zn 2+ binary system. Furthermore, the SMX-biotransformation capability of SDB4 was enhanced at low concentrations of Zn 2+ (below 100 mg·L -1 ). The SMX biotransformation and Zn 2+ adsorption data fitted well with the pseudo-first-order kinetic model, indicating that the two pollutants were in accordance with the same removal rule. N 4 -acetyl-SMX was identified as the main stable transformation product during SMX removal. FTIR analyses revealed that OH, NH 2 , C=O, C-N/N-H, and C-O-C played major roles in the adsorption of Zn 2+ . Our study of the dually functioning strain SDB4 provides a potential application for the simultaneous biological removal of antibiotics and heavy metals.
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高影响因子
RARE METALS
·
2021
·Vol. 41
·期 1
·37-44
·
IF 4.003
Shi Ze-Di,Zhang Le,Ma Yue-Long,Zhou Tian-Yuan,Sun Bing-Heng,Shao Cen,Huang Guo-Can,Yin Shi-Tong,Hou Su-Dan,Yang Zeng-Wang,Chen Hao
银作为一种功能性材料, 由于其优良的导电性而用作于汽车电子的电接触材料。但是, 这种材料极易遭受大气腐蚀问题, 并导致结构的损坏和器件的失效。本文研究了应用于印刷电路板中导电银的腐蚀行为, 并从动力学和机理方面发现了影响材料结构损害最主要的环境因素(SO 2 污染物、温度和相对湿度(RH, Relative humidity))。相较于其他因素, 温度(55 °C)是运行环境中引起导电材料结构变化的最主要因素, 并且表面的腐蚀层厚度达到了最大值, 即 d film =15.56× 10 -5 μm。然而, 在腐蚀过程中, SO 2 污染物和RH促进了腐蚀物质的扩散, 并也会增加腐蚀产物层(Ag 2 S和Ag 2 SO 4 )的厚度。因此, 本文不仅大大增强了人们对于不同环境因素下导电材料结构损害的认识, 同时也为实际生产中的防腐设计和材料选择提供了重要的指导。 Graphical abstract
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JOURNAL OF POLYMER RESEARCH
·
2021
·Vol. 28
·期 9
·1-12
·
IF 3.097
Shi Mengqing,Yang Jin,Wang Xiwen
At present, the research hotspot of polyurethane synthesis focuses on developing bio-based materials with ecological and excellent performance. This paper synthesized a series of castor oil-modified bio-based waterborne polyurethanes (CWBPU) by adjusting the castor oil (CO) content. We used 1,5-pentamethylene diisocyanate and bio-based polyether polyols as base materials. CWBPU had the advantages of environmental protection and degradability, and the bio-based content was as high as 82%. Results showed that, with the increase of CO content from 0% to 8.97%, the tensile strength increased from 3.85 MPa to 8.86 MPa, and the elongation at break gradually decreased from 496.2% to 356.6%. The water contact angle of the films increased by about 30°, and the water absorption rate dropped from 17.44% to 7.6%. Compared with the uncoated leatherette paper, the longitudinal tear strength of the coated flush cover paper was twice that of the base paper, and the transverse tensile strength and burst resistance significantly enhanced.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 25
·6279-6288
·
IF 4.157
Zeng Zhiyao,Tang Juan,Zhang Ming,Pu Shouzhi,Tang Dianping
Herein, a novel signal-on photoelectrochemical (PEC) biosensor with nearly zero background noise (ZBN) was first fabricated to determine the presence of organophosphorus pesticide based on in situ formation of DNA-templated Ag 2 S photoactive materials, accompanied by hybridization chain reaction (HCR) signal amplification. The capture probe (S1) on the gold nanoparticle-modified electrode can hybridize with the aptamer molecule to generate a simple PEC biosensor. In the presence of a target molecule, the aptamer molecule is released on the double-stranded DNA (dsDNA)-modified PEC biosensor. Meanwhile, the capture probe remains on the electrode and can open the DNA hairpins (H1, H2) which are rich in cytosine, to trigger the HCR reaction. The rich “C” strands are uncovered after formation of a long dsDNA polymer strand, which can assemble multiple silver ions (Ag + ) by means of by C–Ag + –C chelation. Then, a large number of Ag 2 S can be generated by challenging with S 2− solution, producing a satisfactory photocurrent signal. The photoactive material is formed in situ, which eliminates the laborious operation. Moreover, the signal can be highly amplified with nearly zero background noise and HCR signal amplification. Under optimal conditions, the ZBN aptasensor exhibited high sensitivity and selectivity, with a low detection limit of 2 pg mL −1 for malathion. Importantly, the sensing platform can also be applied to determine the presence of malathion in real samples. Graphical abstract
In this assay, a novel signal-on photoelectrochemical biosensor with nearly zero background noise was first fabricated to determine the presence of organophosphorus pesticide based on in situ formation of DNA-templated Ag 2 S photoactive materials, accompanied by hybridization chain reaction signal amplification.
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高影响因子
BIOCONTROL
·
2021
·Vol. 67
·期 1
·1-13
·
IF 3.571
Zhang Hao,Wang Jin-Yan,Chen Yi-Juan,Siemann Evan,Ji Xiang-Yun,Jiang Jie-Xian,Wan Nian-Feng
The effects of plant volatiles on parasitoids are important with regards to the tri-trophic interactions among host plants, insect herbivores, and their natural enemies. However, the effects of plant volatiles on the important parasitoid ( Microplitis pallidipes Szépligeti; Hymenoptera: Braconidae) of noctuids have not been determined at the molecular, laboratory, and outdoor scales with complementary approaches. The odorant binding protein 8 of M. pallidipes (MpOBP8) with an open reading frame of 459 bp was cloned and successfully expressed in Escherichia coli (Eubacteriales: Enterobacteriaceae). The purified protein MpOBP8 had a relatively high binding capacity to the plant volatile β- ionone (Ki value was 28.86 μM) in the fluorescence competitive binding assay. Meanwhile, Y-tube olfactometer experiments indicated that β- ionone selectively attracts the parasitic wasp as the ratio of M. pallidipes selecting β- ionone vs. control was 2:1. Likewise, field screenhouse experiments verified the above results as the ratio of M. pallidipes selecting β- ionone vs. control was close to 2:1. This study suggested that β- ionone is a plant volatile that attracts the parasitoid M. pallidipes , and MpOBP8 may play a key role in the discrimination of M. pallidipes in searching for hosts via β- ionone. In general our results suggest that β- ionone has the potential to enhance the biocontrol effect of parasitoids on insect herbivores.
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高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 29
·期 8
·11267-11280
·
IF 4.223
Yang Renhe,He Tingshu
High-fluorine alkali-free liquid accelerator (AF -hf , F − concentration was about 2.31g/L) was still used in engineering because of its low cost, excellent stability, and coagulation-promoting effect. The main purpose of this study was to explore the leaching behavior of fluorine ions in shotcrete for tunnel lining with high-fluorine alkali-free liquid accelerator and whether there was fluoride pollution. The setting time and mechanical properties of cement paste and mortar with AF -hf were tested. Under different environmental conditions, F − leaching concentration from sprayed concrete was studied comparatively. Moreover, XRD and SEM were used to analyze the crystal composition and micro morphology of hydration products. The experimental results showed that with the increase of AF -hf dosage, the setting time of cement paste was greatly shortened, and later strength of mortar and shotcrete could meet the construction requirements. In addition, when the leaching solution type (including Na 2 CO 3 , Na 3 PO 4 , Na 2 SO 4 , and NaNO 3 ) and testing conditions (particle size, soaking temperature, leaching solutions) were different, F − leaching concentration changed regularly, and the minimum value was more than 20 mg/L, which might cause fluorine pollution to groundwater and soil. After shotcrete samples were soaked, the CaF 2 peaks’ intensity was relatively weaker and Ca(OH) 2 decreased obviously. Meanwhile, cement hydration products became looser and abundant of flaky C-S-H gel transformed into fibrous and chained structure.
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高影响因子
JOURNAL OF POLYMER RESEARCH
·
2021
·Vol. 28
·期 10
·1-10
·
IF 3.097
Meng Chihan,Liu Xiucai
Bio-based semi aromatic high temperature polyamide PA5T/56 was synthesized by one-step high temperature and high pressure solution polymerization with bio-based monomer pentanediamine. The non-isothermal crystallization behavior of PA5T/56 was studied by differential scanning calorimetry (DSC). Mo equation was used to describe the non-isothermal crystallization kinetics. The activation energy of non-isothermal crystallization was calculated by Kissinger equation. The effect of benzene ring (T) molar content on the non-isothermal crystallization kinetics was investigated. The crystal morphology was observed by optical polarized microscope (POM) and X-ray diffraction (XRD). The results showed that the non-isothermal crystallization activation energy and absolute crystallinity of nylon 5 T/56 first increased and then decreased with the increased of T molar content from 25% to 32.5%. At the same time, the crystal form of polyamide didn’t change. When the molar content of T was 30%, the number of spherulites was the largest, the size was the smallest, the crystallization rate was the fastest, and the crystallization ability was the highest, which indicated that T can play a role in promoting crystallization in polymer molecular chain.
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ARTHRITIS RESEARCH & THERAPY
·
2021
·Vol. 23
·期 1
·1-11
·
IF 5.156
Han Yi,Wang Jinguang,Li Shufeng,Li Yi,Zhang Yongli,Zhang Ruojia,Zhang Yuang,Fan Huancai,Shi Haojun,Pan Jihong,Song Guanhua,Ge Luna,Wang Lin
Background Isopsoralen (IPRN), one of the active ingredients of Psoralea corylifolia Linn, has anti-inflammatory properties. We attempted to investigate the inhibitory effects of IPRN on rheumatoid arthritis (RA) and characterize its potential mechanism.Methods RA fibroblast-like synoviocytes (FLSs) and mice with collagen-induced arthritis (CIA) were used as in vitro and in vivo models to analyze the antiarthritic effect of IPRN. Histological analysis of the inflamed joints from mice with CIA was performed using microcomputed tomography (micro-CT) and hematoxylin-eosin (HE) staining. RNA sequencing (RNA-Seq), network pharmacology analysis, molecular docking, drug affinity responsive target stability (DARTS) assay, and cellular thermal shift assay (CETSA) were performed to evaluate the targets of IPRN.Results IPRN ameliorated the inflammatory phenotype of RA FLSs by inhibiting their cytokine production, migration, invasion, and proangiogenic ability. IPRN also significantly reduced the severity of CIA in mice by decreasing paw thickness, arthritis score, bone damage, and serum inflammatory cytokine levels. A mechanistic study demonstrated that macrophage migration inhibitory factor (MIF), a key protein in the inflammatory process, was the specific target by which IPRN exerted its anti-inflammatory effects in RA FLSs.Conclusion Our study demonstrates the antiarthritic effect of IPRN, which suggests the therapeutic potential of IPRN in RA.
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高影响因子
CHROMATOGRAPHIA
·
2021
·Vol. 84
·期 11
·1065-1075
·
IF 2.044
Zhang Zixin,Wang Zhida,Zhang Ying,Zhang Yi,Han Lifeng,Tian Fei
We present a facile approach for the direct attachment of cross-linked poly(2-thiopheneacetic acid) onto the surface of magnetite nanoparticles with one single carbon layer (Fe 3 O 4 @1C NPs) via self-assembly oxidative polymerization. The functional Fe 3 O 4 @1C NPs combined with poly(2-thiopheneacetic acid) [Fe 3 O 4 @1C@ poly(Th) NPs] was successfully prepared and used for magnetic solid-phase extraction (MSPE). The influence of the preparation conditions and extraction conditions were investigated in detail. The morphology and size distribution of the monolithic materials were characterized by transmission electron microscopy. Under the optimized experimental conditions, the prepared Fe 3 O 4 @1C@ poly(Th) NPs showed good extraction efficiency for canthin-6-one alkaloids. Furthermore, the results indicated that the binding specificity and selectivity of the Fe 3 O 4 @1C@ poly(Th) NPs might be attributed to electrostatic interactions and pore structure. A MSPE–HPLC method was also developed for quantitative analysis of canthin-6-one alkaloids. The developed method showed good linearity, low limit of detections, low limit of quantifications, and good reproducibility over a wide linear range. To further prove the utility, Fe 3 O 4 @1C@ poly(Th) NPs-MSPE was also used to extract canthin-6-one alkaloids from Eurycoma longifolia stems, with a recovery rate of 87.9–114%. Thus, this method is potentially applicable for the extraction of similar alkaloid compounds from medicinal herb samples.
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高影响因子
EXPERIMENTAL BRAIN RESEARCH
·
2021
·Vol. 239
·期 11
·3315-3325
·
IF 1.972
Yu Tao,Fu Hui,Sun Jing-Jing,Ding Dan-Rui,Wang Hua
To investigate the association of miR-106b-5p with neuroinflammation and microglial activation in a status epilepticus (SE) mouse model. We examined changes in the expression of microRNA-106b-5p (miRNA-106b-5p), repulsive guidance molecule A (RGMa), triggering receptor expressed on myeloid cells 2 (TREM2), and the microglia-related markers interleukin (IL)-1β, IL-4, IL-6, IL-10, inducible nitric oxide synthase (iNOS), and arginase-1 (Arg-1) in the mouse hippocampus of the lithium–pilocarpine-induced SE mouse model. Eighty-four female C57BL/6 mice were randomly divided into a normal control group ( n = 12), and six SE groups ( n = 12/group), which were monitored at 6 h and at 1, 3, 7, 14, and 21 days (d) post-SE induction. Unlike in the dentate gyrus, immunohistochemical staining revealed prominent neuronal swelling at 6 h, significant neuronal loss and apoptosis on day 3, and recovery by day 14 in the hippocampal cornu ammonis (CA)1 and CA3 pyramidal cells in SE mice. We noted elevated levels of miRNA-106b-5p and all microglia-related markers, which peaked at 3 days post-SE, except IL-4, which peaked at 7 days post-SE, indicating inflammation and microglial activation. RGMa and TREM2 levels decreased at 6 h post-SE. All markers but miRNA-106b-5p, RGMa, and TREM2 returned to baseline levels at 21 days post-SE. Dual luciferase reporter gene assay showed that microRNA-106b-5p can interact with RGMa. We observed that miR-106b-5p level increased while both RGMa and TREM2 levels decreased post-SE and showed associations with microglial activation and inflammation in the mouse hippocampus, suggesting their potential as SE therapeutic targets.
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高影响因子
JOURNAL OF POROUS MATERIALS
·
2021
·Vol. 29
·期 1
·97-102
·
IF 2.496
Shen Tieyin,Zhong Mingjun,Feng Lijuan,Sui Zhuyin,Chen Qi
Metal nanoparticles (MNPs)/metal–organic frameworks (MOFs) composites have become a research hotspot because the cooperation between MNPs and MOF can effectively improve the catalytic performance. However, development of a simple method to disperse the tiny MNPs on MOF is still a great challenge. In this work, a AuPdPt/Cu-MOF composite was prepared successfully by a one-step reduction process. First, Cu-MOF with one-dimensional nanorod structure was prepared by using surfactant PVP to control morphological growth. Second, the AuPdPt/Cu-MOF composite was synthesized by reducing the metal precursor to AuPdPt nanoparticles (NPs) supported on the surface of Cu-MOF. Encouragingly, synergistic effect between Au, Pd, and Pt, along with high dispersion of AuPdPt NPs on Cu-MOF nanorods, leading to excellent catalytic activities toward the benzyl alcohol with a conversion rate of 66%. Thus, this simple strategy to design promising MNPs/MOF composite can provide various opportunities in the field of catalysis.
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BMC BIOLOGY
·
2021
·Vol. 19
·期 1
·1-18
·
IF 7.431
Yuan Dongjuan,Li Song,Shang Ziyu,Wan Muchun,Lin Yu,Zhang Yanhua,Feng Yaoyu,Xu Lian,Xiao Lihua
Background Nematodes are a widespread and diverse group comprising free-living and parasitic species, some of which have major detrimental effects on crops, animals, and human health. Genomic comparisons of nematodes may help reveal the genetic bases for the evolution of parasitic lifestyles. Fatty acid and retinol-binding proteins (FARs) are thought to be unique to nematodes and play essential roles in their development, reproduction, infection, and possibly parasitism through promoting the uptake, transport, and distribution of lipid and retinol. However, the evolution of FAR family proteins across the phylum Nematoda remains elusive.Results We report here the evolutionary relationship of the FAR gene family across nematodes. No FAR was found in Trichocephalida species and Romanomermis culicivorax from Clade I, and FAR could be found in species from Clades III, IV, and V. FAR proteins are conserved in Clade III species and separated into three clusters. Tandem duplications and high divergence events lead to variable richness and low homology of FARs in Steinernema of Clade IVa, Strongyloides of Clade IVb, and intestinal parasitic nematodes from Clades Vc and Ve. Moreover, different richness and sequence variations of FARs in pine wood, root-knot, stem, and cyst nematodes might be determined by reproduction mode or parasitism. However, murine lungworm Angiostrongylus and bovine lungworm Dictyocaulus viviparus from Clade Vd have only 3–4 orthologs of FAR. RNA-seq data showed that far genes, especially far-1 and far-2, were highly expressed in most nematodes. Angiostrongylus cantonensis FAR-1 and FAR-3 have low sequence homology and distinct ligand-binding properties, leading to differences in the cavity volume of proteins. These data indicate that FAR proteins diverged early and experienced low selective pressure to form genus-level diversity. The far genes are present in endophyte or root-colonized bacteria of Streptomyces, Kitasatospora sp., Bacillus subtilis, and Lysobacter, suggesting that bacterial far genes might be derived from plant-parasitic nematodes by horizontal gene transfer.Conclusion sData from these comparative analyses have provided insights into genus-level diversity of FAR proteins in the phylum Nematoda. FAR diversification provides a glimpse into the complicated evolution history across free-living and parasitic nematodes.
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高影响因子
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