Human Cell
·
2022
·Vol. 36
·期 1
·312-328
·
IF 4.374
Long Jianwu,Liu Longfei,Yang Xuefeng,Zhou Xiaojun,Lu Xianzhou,Qin Lei
Emerging evidence has uncovered that noncoding RNAs (ncRNAs) contribute to the development of hepatocellular carcinoma (HCC). Nevertheless, the functions of the majority of long ncRNAs (lncRNAs) in HCC are unknown. Here, we intend to probe the function of lncRNA NUTM2A-AS1 in the evolvement of HCC and the related mechanism. Expression levels of lncRNA NUTM2A-AS1, miR-186-5p and KLF7 mRNA in HCC tissues and adjacent non-tumor tissues were monitored. Gain- or loss-of-function assays were utilized to investigate the biological functions of lncRNA NUTM2A-AS1, miR-186-5p and KLF7 in HCC cell lines (including HCCLM3 and Huh7). Western blot was implemented for the detection of the epithelial–mesenchymal transition (EMT)-related proteins (including E-cadherin, Vimentin and Snail), KLF7, Wnt, β-catenin, and stemness-related proteins (Nanog, OCT4, YKL40, and CD133). Furthermore, the targeted associations between lncRNA NUTM2A-AS1, miR-186-5p, and KLF7 were verified by bioinformatics analysis, dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. As a result, lncRNA NUTM2A-AS1 and KLF7 profiles were heightened in the HCC tissues versus adjacent normal tissues, while miR-186-5p had the opposite expression tendency. Up-regulation of lncRNA NUTM2A-AS1 was related to tumor size, advanced tumor stage, and lymph node metastasis of HCC patients. Functionally, overexpression of lncRNA NUTM2A-AS1 heightened HCC cells’ growth, invasion, EMT, and stemness and repressed their apoptosis by activating the Wnt/β-catenin pathway. In contrast, up-regulation of miR-186-5p or inhibition of KLF7 had reverse effects. In vivo, lncRNA NUTM2A-AS1 overexpression facilitated tumor growth and EMT, accompanied by declined miR-186-5p levels and enhanced KLF7 expression. The mechanistic studies revealed that miR-186-5p served as a common target of lncRNA NUTM2A-AS1 and KLF7. As hinted by the rescue experiments, NUTM2A-AS1 partly abated miR-186-5p-mediated anti-tumor effects in HCC cells, whereas KLF7 knockdown reversed the promotive effects of NUTM2A-AS1. LncRNA NUTM2A-AS1 accelerated the evolution of HCC by up-regulating the KLF7/Wnt/beta-catenin pathway through sponging miR-186-5p.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 36
·17188-17202
·
IF 4.682
Ouyang Yuanyong,Zhang Zihao,Huang Wei,Yang Wenzhong,Shen Chuanlai,Chen Yun,Yin Xiaoshuang,Liu Ying
Fluorinated hydroxyapatite-titanium dioxide coating (F-doped HA-TiO 2 ) was deposited in situ onto AZ31 Mg alloy using electrodeposition method. XRD and EDS confirmed the crystal structure and composition of the coating. SEM images showed that the F-doped HA-TiO 2 coating on the surface of AZ31 Mg alloy was extremely uniform and the thickness of coating was reflected by the cross-sectional image. Corrosion behavior of the coating modified Mg alloy was tested by electrochemical and immersion experiments. Compared to the corrosion current density ( I corr ) of the uncoated AZ31 Mg alloy in Hank’s solution, the I corr of F-doped HA-TiO 2 coating was decreased almost 4 orders. The coated AZ31 Mg alloy showed superior biocompatibility, which was strongly demonstrated by the amounts of cells increased in cell culture. The results of hemolysis tests also illustrated that the F-doped HA-TiO 2 coating met the requirements of biomedical materials.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF POROUS MATERIALS
·
2022
·Vol. 30
·期 2
·363-376
·
IF 2.523
Zhu Zaisheng,Zhao Xiaoxi,Liu Yujie,Tian Yong,Wang Xiufang
Hollow mesoporous carbon nanospheres with accessible interior cavity, short diffusion length, and abundant mesopore channels can favor guest encapsulation and facilitate mass diffusion and transfer. However, there still remains great challenge in controllable synthesizing mesoporous carbons with uniform hollow architectures and relatively large mesopores by a straightforward method. Herein, a self-generating dual template synergetic assembly strategy was developed to construct hollow structured nitrogen-doped mesoporous carbon nanospheres (NMCNs) for the first time. Differentiating from conventional emulsion template methods, this synthesis involved the generation of nanoemulsion, which was driven by the interaction between oleic acid and triethanolamine based on the newborn soap effect. The process from alkalic to acidic environment induced the polymerization of the 3D cross-linking framework and established coulomb forces for dual template synergetic assembly. Significantly, the inner-cavity size of NMCNs could be facilely changed by simply varying the addition of oleic acid. Furthermore, varying the ethanol/water volume ratio would regulate the surface roughness of the NMCNs by directing the polymerization mode. This method innovatively coupled the dual template strategy and emulsion polymerization system, providing new insight into the construction of hollow mesoporous materials. Impressively, the hollow NMCNs featured dual pore size (5.2, 8.6 nm), high surface area (≈ 723.48 m 2 /g), and high nitrogen content (12.24 wt%), which delivered enhanced release rate and in vitro bioactivity of insoluble drug curcumin.
✓ 已验证
产品关联
DOI
高影响因子
Food Biophysics
·
2022
·Vol. 18
·期 2
·198-207
·
IF 3.34
Li Ya,Yu Ling,Wang Lifeng,Xiong Wenfei
Frozen storage can greatly improve the shelf life of fresh egg white protein (EWP), but it will also lead to the reduction of protein foaming and can not meet the application needs. Herein, high-intensity ultrasound (HIUS) was used to improve the foam characteristics of EWP in different frozen storage periods. The results showed that compared with fresh egg white, the foaming ability of EWP with different freezing times (0, 3, 7, 14, 21 days) after HIUS treatment (20 kHz, 60% amplitude, 5 min) was significantly improved, especially the EWP after 21 days of frozen storage was doubled by HIUS induction. Furthermore, it was found that the improvement of EWP foamability was mainly due to the enhancement of surface hydrophobicity (H 0 , the value from 263 to 456) and the decrease of apparent viscosity (from 0.506 Pa.s to 0.007 Pa.s), which increased the diffusion rate of protein to the interface (from 0.19 mNm − 1 s − 0.5 to 0.26 mNm − 1 s − 0.5 ) and its adsorption amount (from 35.8 to 43.3%) at the interface. These results showed that HIUS was a simple, efficient and residue free way to improve the foam characteristics of frozen EWP, which has strong promotion and application value.
✓ 已验证
DOI
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2022
·Vol. 29
·期 11
·1-15
·
IF 3.061
Pang Jinying,Tan Dengfeng,Huang Chunyan,Jiang Tanling,Gao Qifeng,Mo Xianzhong,Zhang Shuhua
Nanocomposites have been demonstrated as potential materials for biosensors to biomedicine applications. However, the crystallization mechanism of nanocomposites has not been thoroughly investigated so far. Cellulose nanofiber is used as a new kind of nano-filler to modify the biodegradable polymer to prepare nanocomposites. Banana cellulose nanofibers/polycaprolactone composite membranes (BNCF/PCL) and banana cellulose nanofibers grafted with polycaprolactone/polycaprolactone composite membranes (BGCL/PCL) were prepared, respectively. To explore the effects of BNCF and BGCL on the crystallization properties of PCL. SEM, POM, and DSC were used to study the crystallization of PCL, BNCF/PCL, and BGCL/PCL films. Mo's method was applied to study the non-isothermal kinetics of BNCF/PCL films, Avarmi and Jeziorny's models successfully predict the non-isothermal kinetics of BGCL/PCL films. The results showed that BNCF promoted the crystallization of BNCF/PCL films. The crystallization peak temperature of BNCF/PCL films was 4.11 ℃ higher than pure PCL, and the crystallinity was 5.34% higher than that of pure PCL. The crystallization peak temperature of the BGCL/PCL films decreased slightly, but the crystallinity increased by 3.78%. The crystal growth modes of BNCF/PCL films and BGCL/PCL films were consistent with the three-dimensional growth of heterogeneous nucleation, BNCF/PCL films with average Avrami index n ranging from 2.7 to 3.9, and the BGCL/PCL films with n ranging from 2.5 to 3.2. Furthermore, the micrographs of polarized optical microscopy (POM) supported the kinetics results. This study makes a deep insight into the effect of cellulose nanofiber on the crystallization of biodegradable aliphatic polyesters.
✓ 已验证
产品关联
DOI
高影响因子
CATALYSIS LETTERS
·
2022
·Vol. 153
·期 10
·3074-3091
·
IF 2.936
Faizan Muhammad,Saeed Aamir,Song Piao,Zhang Ruirui,Liu Ruixia,Chang Zhi,Wu Liuzhu,Zhang Manyuan
Vanadium phosphorous oxide (VPO) catalysts have been recognized as heterogeneous catalyst for selective oxidation of low-carbon alkanes i.e., n -butane toward maleic anhydride. This process has widely gained attention globally due to its highly selective and cost-effective process. Herein, we employed Cd 0 . 2 Zn 0 . 8 S as a support for the VPO catalyst and evaluated its n -butane catalytic performance. Compared to the unpromoted catalyst (Blank-VPO), a higher n -butane conversion and MA yield were successfully obtained from the promoted catalyst (Cd 0 . 2 Zn 0 . 8 S@VPO). Interestingly, it reduces the byproducts, i.e., CO 2 and CO emission. Characterization methods such as TGA, XRD, EDS, SEM, FTIR, NH 3 -TPD, XPS, TEM, and Raman spectroscopy were used to characterize the Cd 0 . 2 Zn 0 . 8 S, VPO precursor and catalyst. Graphical Abstract
✓ 已验证
产品关联
DOI
高影响因子
Chinese Medicine
·
2022
·Vol. 17
·期 1
·1-15
·
IF 4.546
Zhang Shiyu,Mi Yinxiao,Ye Tingting,Lu Xiaoyan,Liu Li,Qian Jing,Fan Xiaohui
Background Shenmai injection (SMI), a traditional Chinese medicine (TCM) injection prepared from Red ginseng and Ophiopogon japonicus, is widely used in clinics to treat chemotherapy-induced myelosuppression. Similar to other TCM injections, SMI contains a high amount of carbohydrates (fructose, sucrose, and maltose) in addition to the bioactive substances, specifically ginsenosides (Rg1, Re, and Rb1). To date, the role of these carbohydrates in the hematopoietic function of SMI remains unclear.PurposeWe aimed to investigate the hematopoietic effects and potential mechanisms of SMI and its components, focusing on the carbohydrates present in SMI.Experimental design/methodsFirst, we evaluated the hematopoietic effect of SMI on 5-fluorouracil (5-FU)-induced myelotoxicity in a tumor-bearing mouse model. Then we prepared mixtures of ginsenosides and carbohydrates according to their proportions in SMI and evaluated their hematopoietic function in mice with 5-FU-induced myelosuppression. Finally, hematopoiesis-related molecular networks were built based on RNA sequencing (RNA-seq) of the bone marrow stromal cells (BMSCs), and the potential mechanisms of carbohydrates and ginsenosides were evaluated.Results SMI attenuated 5-FU-induced myelotoxicity in tumor-bearing mice. Both ginsenosides and carbohydrates increased the bone marrow nucleated cell (BMNC) count and improved the bone marrow morphology in myelosuppressive mice; they promoted the proliferation of BMSCs derived from those myelosuppressive mice. Bioinformatics analyses revealed ECM-receptor interaction, Hippo signaling, and Wnt signaling are common pathways regulated by both ginsenosides and carbohydrates; Gstt1, Gstp2, Gsta4 and Oplah in Glutathione metabolism pathway and Cd19, Cd79a, and Cd79b in B cell receptor pathway are uniquely regulated genes related to carbohydrates but not ginsenosides.Conclusion sCarbohydrates may collaborate with ginsenosides and contribute to the hematopoietic function of SMI. Carbohydrates could be considered as a bioactive component in this TCM injection.Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2022
·Vol. 33
·期 1
·177-184
·
IF 2.525
Sang Fuming,Xiong Tiedan,Wang Weijie,Pan Jianxin,Shi Huahua,Zhao Yan
A novel fluorescence probe for the detection of Al 3+ was developed based on methionine protected gold nanoclusters (Met-AuNCs). A fluorescent Schiff base (an aldimine) is formed between the aldehyde group of salicylaldehyde (SA) and the amino groups of Met on the AuNCs, and developed for selective detection of Al 3+ in aqueous solution. Al 3+ can strongly bind with the Schiff base ligands, accompanied by the blue-shift and an obvious fluorescence emission enhancement at 455 nm. The limits of detection (LODs) of the probe are 2 pmol L −1 for Al 3+ . Moreover, the probe can successfully be used in fluorescence imaging of Al 3+ in living cells (SHSY5Y cells), suggesting that the simple fluorescent probe has great potential use in biological imaging.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2022
·Vol. 13
·期 1
·1-11
·
IF 17.694
Yu Dejian,Cao Fei,Liao Jinfeng,Wang Bingzhe,Su Chenliang,Xing Guichuan
Mixed-dimensional 2D/3D halide perovskite solar cells promise high stability but practically deliver poor power conversion efficiency, and the 2D HP component has been held as the culprit because its intrinsic downsides (ill charge conductivity, wider bandgap, and strong exciton binding) were intuitively deemed to hinder carrier transport. Herein, we show that the 2D HP fragments, in fact, allow free migration of carriers in darkness but only block the carrier transport under illumination. While surely limiting the photovoltaic performance, such photoinduced carrier blocking effect is unexplainable by the traditional understanding above but is found to stem from the trap-filling-enhanced built-in potential of the 2D/3D HP interface. By parsing the depth-profile nanoscopic phase arrangement of the mixed-dimensional 2D/3D HP film for solar cells and revealing a photoinduced potential barrier up to several hundred meV, we further elucidate how the photoinduced carrier blocking mechanism jeopardizes the short-circuit current and fill factor.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Light-Science & Applications
·
2022
·Vol. 11
·期 1
·1-11
·
IF 20.257
Li Pengfei,Xue Shanshan,Sun Lu,Zong Xupeng,An Li,Qu Dan,Wang Xiayan,Sun Zaicheng
Carbon dots (CDs) as the advancing fluorescent carbon nanomaterial have superior potential and prospective. However, the ambiguous photoluminescence (PL) mechanism and intricate structure-function relationship become the greatest hindrances in the development and applications of CDs. Herein, red emissive CDs were synthesized in high yield from o -phenylenediamine (oPD) and catechol (CAT). The PL mechanism of the CDs is considered as the molecular state fluorophores because 5,14-dihydroquinoxalino[2,3-b] phenazine (DHQP) is separated and exhibits the same PL properties and behavior as the CDs. These include the peak position and shape of the PL emission and PL excitation and the emission dependence on pH and solvent polarity. Both of them display close PL lifetime decays. Based on these, we deduce that DHQP is the fluorophore of the red emissive CDs and the PL mechanism of CDs is similar to DHQP. During the PL emission of CDs, the electron of the molecule state can transfer to CDs. The formation process of DHQP is further confirmed by the reaction intermediates (phthalazine, dimers) and oPD. These findings provide insights into the PL mechanism of this type of CDs and may guide the further development of tunable CDs for tailored properties.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2022
·Vol. 16
·期 1
·55-62
·
IF 3.498
Li Jiale,Li Haoyang,Pei Shizeng,Kang Na,Zhang Guomei,Zhang Caihong,Shuang Shaomin
In this work, a new dye (DAC-CPO) with typical donor-π-acceptor (D-π-A) structure was designed, which could enter the IB subdomain of human serum albumin (HSA) and form the host–guest supramolecular system. Formation of the HSA/DAC-CPO complex was confirmed by the competitive displacement experiments and the molecular-docking study. DAC-CPO alone had no response to sulfur dioxide (SO 2 ), while the HSA/DAC-CPO complex displayed an obvious increased fluorescence response and remarkable blue-shifted emission to SO 2 . The sensitive detection method of SO 2 in aqueous solution was developed with the linear range of 0.5 ~ 4.0 μM. Spectrum data of polarity and viscosity showed that formation of the complex hindered the internal charge-transfer (ICT) process and restricted the rotation of the dye, resulting in the speed increase of reaction between the dye and SO 2 . In addition, the HSA/DAC-CPO complex had also been successfully used for rapid detection of SO 2 in actual samples.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2022
·Vol. 33
·期 26
·20981-20991
·
IF 2.779
Yan Gui-wei,Liu Qing-qing,Fang Bi-jun,Chen Zhi-hui
Mg doped (Ba 0.98 Li 0.02 )TiO 3 energy storage ceramics with antiferroelectric-like double hysteresis loops were prepared using solid-state reaction method. XRD patterns indicated that tetragonal phase structure of the pure (Ba 0.98 Li 0.02 )TiO 3 ceramic was destroyed and the orthorhombic phase structure appeared with Mg doping. The ratio of orthorhombic/tetragonal phase increased with Mg content increasing, and the value of polarization was in direct proportion to the ratio of orthorhombic/tetragonal phase. The breakdown strength of (Ba 0.98 Li 0.02 )(Mg x Ti 1− x )O 3 ceramics was improved from 60.97 kV/cm at x = 0 to 103.66 kV/cm at x = 0.04. The optimum energy storage properties of (Ba 0.98 Li 0.02 )(Mg x Ti 1− x )O 3 ceramics were obtained with energy storage density of 0.76 J/cm 3 at 102.5 kV/cm when x = 0.04, which is nearly 2.3 times larger than the pure (Ba 0.98 Li 0.02 )TiO 3 ceramic, and the energy storage efficiency was improved from 64 to 87%.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）