COLLOIDS AND SURFACES B-BIOINTERFACES
·
2022
·
IF 5.999
Qiang Zhang,Shengbao Duan,Yinong Huang,Jingjing Tian,Jia Hu
By integrating the fluorescence of quantum dots (QDs) and Mn 2+ -pefloxacin mesylate (Mn 2+ -pefloxacin), a new type of dual-band fluorescence biosensor for high-efficiency and sensitive determination of double-stranded DNA (dsDNA) is developed. The biosensor is based on the fluorescence "OFF-ON" mode of both QDs and QDs-Mn 2+ -pefloxacin. The Mn 2+ -pefloxacin complex can quench the QDs fluorescence via photoinduced electron transfer (PET), and its fluorescence is also quenched. Due to the specificity and strong binding affinity of dsDNA for the Mn 2+ -pefloxacin complex, it can break the low fluorescent QDs-Mn 2+ -pefloxacin and restore the fluorescence of QDs and Mn 2+ -pefloxacin complex in their respective bands. Therefore, the dual-band fluorescence quantitative detection of dsDNA by QDs-Mn 2+ -pefloxacin can be achieved, while bovine serum albumin , single-stranded DNA, and bio-related ions do not yield similar results. Furthermore, the possible reaction mechanisms are systematically discussed. The detection limits (3δ/K) of herring sperm (hs) DNA in the fluorescence recovery bands of QDs and Mn 2+ -pefloxacin complex are 0.0142 and 0.0465 μg/mL, respectively. The developed biosensor was used for dsDNA detection in synthetic samples, and desirable results are obtained.
研究领域:
生物化学
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分子生物学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Siqi Zhao,Yaxue Jia,Anping Wang,Jinlan Yang,Li Yang
We present a novel dual-mode DNA-walker based optical fiber biosensor (DMDW-Opt biosensor) for sensitive assay of micro-RNAs in bio-samples. In the sensor system , we develop a new strategy for the cascade amplification, DNA-walker/rolling cycle amplification (RCA), by the use of the residue track of the walker. The strategy can significantly improve the response of the sensor and avoid any tedious operation procedure. Dual-mode readouts, i.e., fluorescence and chemiluminescence , are measured independently without interfering with each other to achieve reliable and accurate analysis. Optical fibers with the surface modified by gold nanoparticles are utilized as the support for fabrication of the sensor, which would be benefit for developing miniaturized and portable sensing devices. The performance of the proposed method is evaluated by using micro-RNAs (MiR-155 and MiR-21) as the analytical target. The method is successfully applied for accurate determination of micoRNAs in human serum and MCF-7 cells. Our method can perform sensitive assays of MiR-155 with limit-of-detection as low as 97.72 fM and 11.22 fM, MiR-21 with limit-of-detection as low as 107.15 fM and 8.32 fM for the fluorescence- and the chemiluminescence-readout respectively, and the biosensor exhibits excellent specificity, reproducibility and storage stability, indicating its valuable potential applications for sensing trace-amount targets in complicated real samples.
研究领域:
生物传感
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纳米技术
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分子生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 32
·期 12
·2107267
·
IF 18.808
Jianpu Tang,Junhan Ou,Chenxu Zhu,Chi Yao,Dayong Yang
Deoxyribonucleic acid (DNA) hydrogel has been synthesized generally via the molecular interaction of either DNA base-pairing, covalent bonding, or physical entanglement. To surmount the limitations of DNA as the sole module in materials, new assembly modes are desired to incorporate to expand the functionalities of DNA hydrogel. Herein, a supramacromolecular assembly-based strategy is reported to prepare a hybrid DNA hydrogel via interfacial assembly of DNA chains and upconversion nanoparticles (UCNPs), remarkably of which the synthesis process finished within one second, termed as flash synthesis. The flash synthesis is accomplished through the electrostatic attraction, interfacial assembly, and cross-linking of DNA chains on the surface of UCNPs. The length of DNA and the crystalline form of UCNPs are proven to be essential factors in the construction of a hydrogel network. The rationally designed DNA endowed hydrogel with functions to precisely recognize and isolate specific cells, and lanthanide ions-doped UCNPs protected cells from the damage of near-infrared irradiation through the upconversion effect. It is envisioned that the flash synthesis provides a new mode to prepare the DNA hydrogel and expands the functionalities to realize more real-world applications.
研究领域:
材料科学
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生物技术
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纳米技术
·
细胞生物学
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DOI
高影响因子
ANALYST
·
2018
·Vol. 143
·期 4
·914-919
·
IF 3.864
Yaoqi Wang,Jiaxue Gao,Xianying Meng,Zhenxin Wang
Highly accurate analysis of single-nucleotide polymorphisms (SNPs) plays an important role in both disease diagnostics and personalized medicine development. In this work, a DNA microarray-based resonance light scattering (RLS) assay has been developed for multiplexed detection of papillary thyroid carcinoma (PTC) related mutation points including BRAFV600E (t1m), NRAS codon 61 (t2m), TERT promoter g.1295228 (t31m) and TERT promoter g.1295250 (t32m) with high sensitivity and selectivity by the attachment of polyvalent ssDNA modified 13 nm gold nanoparticles (ssDNAs@GNPs) followed by silver deposition for signal enhancement. The microarray-based RLS assay provides a detection limit (S/N = 3) at the sub-nanomolar level for the target ssDNAs and determines allele frequencies as low as 0.2% for t1m, 0.2% for t2m, 0.5% for t31m, and 0.5% for t32m in the cocktail of target ssDNAs, respectively. The practicability of the DNA microarray-based RLS assay is demonstrated by profiling of t2m in 50 clinical thyroid tissue samples of PTC patients, and satisfactory results are obtained.
研究领域:
癌症诊断
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分子生物学
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纳米技术
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遗传学
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产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2022
·Vol. 37
·期 8
·1275-1283
·
IF 2.613
Shuyun Bi,Xu Li,Zhixin Ren,Bin Yang,Yuting Wang,Fengming Zhang,Yue Yuan,Di Shao,Rui Zhao
The binding of tiamulin with calf thymus DNA was systematically investigated using multispectroscopy and molecular modelling techniques. For DNA, once tiamulin was added, viscosity ( η ) and melting temperature ( T m ) both exhibited an uptrend. The fluorescence performance of the tiamulin–DNA complex did not change with the ionic strength changes. The binding constant ( K a ) of tiamulin for single-stranded DNA (ssDNA, 1.48 × 10 4 M −1 ) was obviously higher than that for double-stranded DNA (dsDNA, 9.51 × 10 3 M −1 ) at 291 K. The helix structure became looser and the base stack force became stronger for DNA due to the presence of tiamulin as seen from circular dichroic (CD) spectra. The intercalation binding mode of tiamulin with DNA was disclosed. Molecular modelling also revealed tiamulin inserting into the base pairs with the lowest binding free energy of −18.73 kJ mol −1 using van der Waals forces as well as hydrogen bonds.
研究领域:
生物化学
·
药物化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL SCIENCES
·
2021
·Vol. 37
·期 11
·1525-1531
·
IF 2.081
Zhifang DING,Yue LI,Ying BAO,Kexin HAN,Iparguli TUREPU,Guixin LI
A signal-on supersandwich type of electrochemiluminescence (ECL) DNA biosensor was developed based on the poly(aniline-luminol) nanowires (PALNWs) modified electrode and enhancement of ferrocene (Fc) on ECL of luminol. Aminated capture DNA was covalently linked to the PALNWs on the electrode surface by the crosslinking of glutaraldehyde. In presence of target DNA, its 3′ terminus hybridizes with the capture probe and the 5′ terminus hybridizes with ferrocene labeled DNA (Fc-DNA) to form a long DNA concatamer supersandwich structure. The ECL intensity of the prepared biosensor was clearly improved by increasing the concentration of target DNA due to the enhancement of ferrocene on luminol ECL. The difference of the ECL intensity in the absence and presence of target DNA was used to monitor the hybridization event. The difference of ECL linearly increased with the logarithm of target DNA concentration in the range from 1.0 × 10−16 – 1.0 × 10−8 mol L−1 with a detection limit of 5.8 × 10−17 mol L−1. The sensor had high sensitivity and wide linear relationship for the detection of target DNA.
研究领域:
分析化学
·
生物传感技术
✓ 已验证
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2020
·Vol. 30
·期 27
·2000391
·
IF 16.836
Jingjiao Wu,Huiyi Liang,Yuanchao Li,Yi Shi,Massimo Bottini,Yongming Chen,Lixin Liu
A high concentration of cell-free DNA (cfDNA) in joints is considered a disease causative agent of rheumatoid arthritis (RA) and cfDNA scavenging has been regarded as an efficient therapeutic avenue. Cationic polymers can hamper progression of joint inflammation in a rat model of RA by scavenging cfDNA; however, they may cause systemic toxicity due to the strong positive charges. To reduce the toxicity, herein a library of cationic nanoparticles (cNPs) of block copolymer micelles is developed and the effects of structure and surface composition on cNP efficacy to bind nucleic acids, toxicity, and therapeutic activity on a collagen induced arthritis (CIA) rat model of RA are assessed. The library includes cNPs with a homoshell from poly(lactic- co -glycolic acid)- block -poly(2-(dimethylamino)ethyl methacrylate) (PLGA- b -PDMA) block copolymers and cNPs with a mixed shell of poly(ethylene glycol) (PEG) and PDMA by coself-assembling PLGA- b -PDMA and PLGA- b -PEG block copolymers. Relatively to the homoshell cNPs, introduction of PEG segments translates into a lower DNA binding efficacy while preserving ability to hamper joint inflammation. Moreover, they show a greater accumulation and longer retention at the inflamed joints, allowing a lower administration frequency. In conclusion, this work shows that the therapeutic index of cationic materials can be tuned by introducing surface neutral moieties.
研究领域:
纳米技术
·
生物材料
·
药学
·
风湿病学
·
生物医学工程
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DOI
高影响因子
Oncotarget
·
2017
·
IF 5.168
Hongzhen Bai,Min Wu,Hongjian Zhang,Guping Tang
Cell exposure to atmospheric polycyclic aromatic hydrocarbons (PAHs) is closely associated with DNA damage and genomic instability. We assessed the mechanisms of chronic and acute PAH exposure-induced genotoxicity in two human lung epithelial cell lines, A549 and NC-H1975. We sampled atmospheric PAHs at the Xixi Campus, Zhejiang University in Hangzhou, China during August (non-haze episode) and November (haze episode) 2015. We identified vehicle emissions as a dominant anthropogenic PAH source in our study. PAHs were extracted according to the United States Environmental Protection Agency Method TO-13A. We found that chronic PAH exposure saturated lung cell xenobiotic metabolic pathways, promoting intercellular reactive oxygen species production and accumulation. Chronic alteration of the cellular redox status resulted in DNA damage and genomic instability. Chronic PAH exposure also perturbed the cellular DNA damage response, inducing S phase arrest and inhibiting apoptosis. Dysregulation of PAH metabolism and the DNA damage response altered cellular homeostasis and increased cell susceptibility to subsequent PAH exposures, thereby enhancing the likelihood of genomic mutation and instability.
研究领域:
毒理学
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分子生物学
·
环境科学
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
Small
·
2014
·Vol. 10
·期 8
·1500-1503
·
IF 7.514
Xiang Ran,Fang Pu,Jinsong Ren,Xiaogang Qu
Graphical Upconversion applied in logic gate : The Upconversion nanoparticles (UCNPs) give multi emissions when excited by midrange IR radiation. A ternary logic system is designed which combines the unique characters of UCNPs with the ensemble of DNA. By regulating the emission of UCNPs, this logic system involvs more than two states, and the density of information can be increased.
研究领域:
纳米技术
·
生物医学工程
·
光电子学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Pei Liu,Kexin Zhang,Ranran Zhang,Huanshun Yin,Yunlei Zhou,Shiyun Ai
Abnormal level of DNA methyltransferase (MTase) – mediated DNA methylation is closely related with cancer and bacterial diseases. Herein, a novel strategy based on the keypad lock of duplex DNA modified meso-SiO 2 @Fe 3 O 4 was developed for colorimetric assay of Dam MTase activity. When the Dam MTase was introduced, the duplex DNA can be methylated at a palindrome sequence of 5′-GATC-3′ and cleaved by the methylation-sensitive restriction endonuclease Dpn I. Due to the instability of the newly formed DNA fragment, the hybrid will separated into a single-stranded DNA. Then the keypad lock will open, and the catalytic reaction of TMB and H 2 O 2 can be initiated through the pores of meso-SiO 2 @Fe 3 O 4 , and a high color signal can be clearly observed by the naked eye. Contrarily, without Dam MTase, the catalytic reaction will not be initiated, and result no color signal. The proposed method exhibited a wide dynamic range with a low detection limit of 0.73 U/mL. Additionally, this way can be performed in human serum with satisfying recovery. And the inhibition of Dam MTase can also be well demonstrated by using paclitaxel as a model. Therefore, the designed way not only provides a platform for monitoring Dam MTase activity, but also useful for further application in disease diagnosis and drug discovery.
研究领域:
生物化学
·
分子生物学
·
癌症研究
·
药物发现
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2019
·Vol. 11
·期 45
·21856-21866
·
IF 6.97
Hongmei Geng,Chunyang Zhou,Chunlei Guo
In this paper, we propose a strategy involving coupling DNA structural nanoswitches with toehold mediated strand displacement for constructing novel DNA-based digital comparator (DC) logic systems, which are a basic part of traditional electronic computers and can compare whether two or more input numbers are equal. However, when the number of DC inputs is increased to a certain level, the speed and quality of the computing circuit can be affected because of the limitations of conventional electronic computers when it comes to handling large-scale quantities of data. To solve this problem, in this work, we introduce a multi-input to multi-output DNA switch-based platform that can enable complex DC logical comparison. These multifunctional DNA-based switches, each including two hairpin-shaped molecular beacons and a G4/NMM complex, were used as platforms for the step-by-step realization of 2-3 DC, 3-3 DC, and 4-3 DC logic operations. Also, experiments were designed to further verify the excellent selectivity, achieving single-base mismatch operations with the digital comparator. Based on our design, comparators (“>”, “<” and “=”) can be realized. Our prototype can inspire new designs and have intelligent digital comparator and in-field applications.
研究领域:
生物化学
·
分子生物学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2016
·
IF 2.74
Muhammad Asim Akhtar,Sara Riaz,Akhtar Hayat,Muhammad Nasir,Nawshad Muhammad,Abdur Rahim,Mian Hasnain Nawaz
trans -Porphyrin containing phenyl group at both ends was synthesized via corresponding dipyrromethanes (DPM) in the presence of TFA and DDQ. The polyethyleneglycole (PEG) chains were attached at the trans positions of ABA type porphyrinic moiety. The prepared PEGylated porphyrin (with PEG arms at the periphery) P-(PEO) 2 was self-assembled into spheres of uniform diameter while their DMF solution was dialyzed into water. Furthermore, the addition of fullerene (C 60 ) to P-(PEO) 2 caused complexation between porphyrin and fullerene which lead to interesting and trenchant morphology having worm like lateral aggregates. The assembly process and the as-prepared edifices were characterized using UV–vis absorption spectroscopy and the morphology of these assemblies was investigated using transmission electron microscope (TEM). Furthermore, the DNA binding with porphyrin aggregates has been demonstrated via electrochemical (cyclic voltammetric) response of screen printed electrodes modified . The obvious increase in redox current disclosed the intermolecular interactions of the adducts with the DNA. These studies of unprecedented adducts can further be explored for potential applications in biosensors .
研究领域:
生物化学
·
材料科学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）