WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2022
·Vol. 38
·期 6
·1-10
·
IF 4.253
Liang YaoYao,Luo Juan,Yang Chenhao,Guo Shuning,Zhang Bowen,Chen Fengqianrui,Su Kairui,Zhang Yulong,Dong Yi,Wang Zhihao,Fu Hongda,Sui Guangchao,Wang Pengchao
Hydroxy-mandelic acid (HMA) is widely applied in pharmaceuticals, food and cosmetics. In this study, we aimed to develop an allosteric transcription factors (aTFs) based biosensor for HMA. PobR, an aTF for HMA analog 4-hydroxybenzoic acid, was used to alter its selectivity and create novel aTFs responsive to HMA by directed evolution. We established a PobR mutant library with a capacity of 550,000 mutants using error-prone PCR and Megawhop PCR. Through our screening, two mutants were obtained with responsiveness to HMA. Analysis of each missense mutation indicating residues 122–126 were involved in its PobR ligand specificity. These results showed the effectiveness of directed evolution in switching the ligand specificity of a biosensor and improving HMA production.
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高影响因子
FIBERS AND POLYMERS
·
2022
·Vol. 23
·期 5
·1225-1234
·
IF 2.347
Li Siqi,Yin Jing,Xu Lan
As biomaterials with excellent biocompatibility, biodegradation and low toxicity, poly(ε-caprolactone) (PCL) and poly(lactic-co-glycolic acid) (PLGA) are extensively applied as wound dressing and surgical suture in biomedical field. In this paper, an oblique section free surface electrospinning (OSFSE) apparatus was utilized to prepare high-quality and high-output ZnO/PLGA/PCL nanofiber membranes (NFMs) for antibacterial materials, which could significantly increase the production of ZnO/PLGA/PCL NFMs from 0.01–0.1 g/h of traditional electrospinning to 30.84 g/h of OSFSE. The influences of the weight proportion of PLGA and PCL on the viscosity and conductivity of electrospinning solutions along with the yield, morphology and wettability of PLGA/PCL NFMs were researched, and the optimum weight ratio of 6:4 was determined. Then the effects of ZnO contents on the electrospinning solution properties as well as the yield, morphology, structure, wettability, mechanical property and antibacterial performance of ZnO/PLGA/PCL NFMs with the optimal ratio of PLGA and PCL were studied. The results illustrated that additive nano-ZnO could improve markedly the antibacterial effect of NFMs, and the NFMs with 3 wt% nano-ZnO had excellent antibacterial effect on Escherichia coli (95.3 %) and Staphylococcus aureus (95.7 %).
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Food and Bioprocess Technology
·
2022
·Vol. 15
·期 5
·1101-1114
·
IF 5.581
Javed Miral,Belwal Tarun,Ruyuan Zhang,Xu Yanqun,Li Li,Luo Zisheng
Camellia oleifera shells are a noteworthy by-product often considered as agricultural waste and discarded rather than efficiently used. The extraction of bioactive compounds from shells using green extraction process can be a better approach for waste valorization. Also, demand for novel and green extraction method is required to avoid organic solvents, high cost, and environmental problems. Nanobubbles (NBs) can be used for extraction purposes due to their high specific area and internal pressure. In this study, a novel method using nanobubbles (NBs) solution coupled with ultrasonication to extract phytochemicals from Camellia Oleifera shells (COSs) was first explored. The characterization was performed to measure the size, zeta potential, and surface tension of NBs stabilized by biosurfactant in this case rhamnolipid (Rh). The produced NBs were of size 100 nm, having a surface tension of 35.26 ± 0.31 mN/m −1 . Single-factor experiments were performed to determine the significant variables. The Box–Behnken design optimized the ultrasonic power, ultrasonic time, and surfactant concentration to maximize the phytochemicals extraction. NBs solution coupled with ultrasonication (US) leads to a higher extraction yield of total phenolic (34.42 mg of gallic acid equivalent per gram of dry weight (mg GAE/g DW)) and flavonoid content (23.36 mg of rutin equivalent per gram of dry weight (RE/g DW)), as compared to the conventional extraction method. In addition, in vitro antioxidant activity was also increased in NBs solution and NBs’ solution–US extract compared with the conventional solvent method. The morphological and microstructural changes under NBs solution and ultrasonication were also determined to provide a possible extraction mechanism. The present experiment provides a novel extraction method and an overall green perspective of agro and food waste valorization.
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JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
·
2022
·Vol. 37
·期 2
·149-154
·
IF 1.271
Sun Jiyang,Li Yiqiu,Guo Xiangxin
Li/garnet/LiFePO 4 solid-state battery was fabricated. The cathode contains LiFePO 4 , Ketjen black, poly(vinylidene fluoride):LiTFSI polymer as active material, electric conductor and Li-ion conducting binder, respectively. Polyvinylpyrrolidone was added into the cathode to improve cathode/electrolyte interfacial performance. When combined with polyvinylpyrrolidone additive, poly(vinylidene fluoride):polyvinylpyrrol idone:LiTFSI blend forms, and the cathode/electrolyte interfacial resistance reduces from 10.7 kΩ to 3.2 kΩ. The Li/garnet/LiFePO 4 solid-state battery shows 80% capacity retention after 100 cycles at 30 °C and 0.05 C. This study offers a general strategy to improve cathode/electrolyte interfacial performance and may enable the practical application of solid-state Li-metal batteries.
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Bioresources and Bioprocessing
·
2022
·Vol. 9
·期 1
·1-15
·
IF 4.983
Cheng Guoqing,Ding Huili,Chen Guanglin,Shi Hongjie,Zhang Xu,Zhu Minglong,Tan Wensong
Sulfate-containing wastewater has a serious threat to the environment and human health. Microbial technology has great potential for the treatment of sulfate-containing wastewater. It was found that nano-photocatalysts could be used as extracellular electron donors to promote the growth and metabolic activity of non-photosynthetic microorganisms. However, nano-photocatalysts could also induce oxidative stress and damage cells. Therefore, the interaction mechanism between photosynthetic nanocatalysts and non-photosynthetic microorganisms is crucial to determine the regulatory strategies for microbial wastewater treatment technologies. In this paper, the mechanism and regulation strategy of cadmium sulfide nanoparticles (CdS NPs) on the growth of sulfate-reducing bacteria and the sulfate reduction process were investigated. The results showed that the sulfate reduction efficiency could be increased by 6.4% through CdS NPs under light conditions. However, the growth of Desulfovibrio desulfuricans C09 was seriously inhibited by 55% due to the oxidative stress induced by CdS NPs on cells. The biomass and sulfate reduction efficiency could be enhanced by 6.8% and 5.9%, respectively, through external addition of humic acid (HA). At the same time, the mechanism of the CdS NPs strengthening the sulfate reduction process by sulfate bacteria was also studied which can provide important theoretical guidance and technical support for the development of microbial technology combined with extracellular electron transfer (EET) for the treatment of sulfate-containing wastewater.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2022
·Vol. 45
·期 6
·1075-1088
·
IF 3.434
Wei Kaixin,Wu Xiaomei,Ma Baodi,Li Zhi,Xu Yi
A series of nickel-incorporated SBA-15 mesoporous molecular sieves (Ni-SBA-15) were prepared as support for the immobilization of his-tagged recombinant Microbacterium esterase. The Ni-SBA-15 could strongly and specific absorb the his-tagged esterase from cell disrupted supernatant. It was found that the nickel amount in Ni-SBA-15 has dramatic influence on the activity and thermo-stability of immobilized enzyme, while the kinds of nickel precursor had little effect on enzyme stability. The morphology, chemical composition and structure of the best support NiCl 2 -SBA-15 (Ni-SBA-15 prepared from NiCl 2 precursor) were characterized by various spectroscopy techniques. The immobilized esterase retained full activity of free esterase and showed high immobilized yield (> 90%) with higher thermo-stability, pH stability and organic solvent resistance compared with free enzyme. The optimum reaction temperature increased from 35 to 40 °C and the optimal reaction pH moved from 10.0 to 8.0 after enzyme immobilization. The immobilized esterase exhibited excellent storage stability and keeping 92% of the initial activity after 30 days’ storage at 25 °C. In addition, the immobilized esterase had excellent reusability for the synthesis of key chiral intermediate of d -biotin and the substrate conversion could still keep 100% after 13 cycles continuously. Finally, optical pure (4 S , 5 R )-hemiester was obtained in 80.8% isolated yield and 99% purity in the gram preparative scale.
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Science China-Materials
·
2022
·Vol. 65
·期 10
·2665-2674
·
IF 8.64
Zhou Ya-Nan,Wang Feng-Ge,Zhen Yi-Nuo,Nan Jun,Dong Bin,Chai Yong-Ming
The correlation between crystal facets and electronic configurations of perovskite is closely related to the intrinsic activity for water splitting. Herein, we proposed a unique molten-salt method (MSM) to manipulate the electronic properties of LaCoO 3 by fine-tuning its crystal facet and atomic doping. LaCoO 3 samples with oriented (110) (LCO (110)) and (111) (LCO (111)) facets were motivated by a capping agent (Sr 2+ ). Compared with the LCO (111) plane, the LCO (110) and Sr-doped LCO (111) (LSCO (111)) planes possessed higher O 2p positions, stronger Co 3d–O 2p covalencies, and higher Co spin states by inducing CoO 6 distortion, thus leading to superior oxygen evolution reaction (OER) and hydrogen evolution reaction (HER) performances. Specifically, the overpotentials at 10 mA cm −2 were 299, 322, and 289 mV for LCO (110), LCO (111), and LSCO (111), respectively. In addition, the (110) crystal facet and Sr substitution bestowed enhanced stability on LaCoO 3 due to the strengthened Co–O bonding. The present work enlightens new avenues of regulating electronic properties by crystal facet engineering and atom doping and provides a valuable reference for the electron structure-electrocatalytic activity connection for OER and HER.
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Nano-Micro Letters
·
2022
·Vol. 14
·期 1
·1-13
·
IF 23.655
Jiang Meifang,Mu Pengzhou,Zhang Huanrui,Dong Tiantian,Tang Ben,Qiu Huayu,Chen Zhou,Cui Guanglei
The double-network binder shows excellent adhesive and self-healing abilities, which help suppress electrode volume expansion and stabilize the electrode interface upon cycling.
This binder induces a Li 3 N/LiF-rich solid electrolyte interface layer, which can suppress continuous electrolyte decomposition.
Superior electrochemical performance can be achieved in Si/Li half cells and LiNi 0.8 Co 0.1 Mn 0.1 O 2 /Si full cells, even with a high loading of Si electrode.
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CHEMICAL PAPERS
·
2022
·Vol. 76
·期 7
·4379-4392
·
IF 2.146
Zhang Huixian,Hu Xizhou,Xia Hong,Zhou Youxiang,Peng Lijun,Wu Jianyuan,Peng Xitian
Phenoxy carboxylic acid herbicides (PCAs) are widely used in agricultural production. However, due to their potential threat to human health, it is necessary to develop efficient analysis method for monitoring PCA concentration in environment and food samples. Based on dispersive micro-solid phase extraction (D-μ-SPE) with amine-functionalized metal–organic framework MIL-101(Fe)-NH 2 , a selective, sensitive, and cost-efficient method was proposed for the extraction of PCAs from environmental water and rice samples in this study. From the perspective of adsorption kinetics and isotherms, interaction mechanisms between MIL-101(Fe)-NH 2 and PCAs were investigated. Key parameters of D-μ-SPE procedure including adsorbent dosage, solution pH, ionic strength, eluting solution, and washing solutions were optimized. Using of small amount of MIL-101(Fe)-NH 2 could achieve desirable extraction effect due to its large surface area and multiple interactions with PCAs. Under the optimized D-μ-SPE conditions, calibration curves of 8 PCAs were linear within a concentration range of 0.05–5 ng/mL for water samples ( r 2 > 0.9898) and 0.5–50 ng/g for rice samples ( r 2 > 0.9879). Limits of detection of water and rice samples were as low as 0.004–0.008 ng/mL and 0.05–0.1 ng/g, respectively. The recoveries of 8 PCAs ranged from 83.0 to 109.3%. Both inter- and intra-day relative standard deviations were less than 10.3%. Our proposed method was successfully employed in actual environmental water and rice samples.
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JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 13
·7056-7067
·
IF 4.682
Guan Meng,Xuan Yang,Gao Yating,Liu Yining,Zhang Shubiao
Bacterial infections are closely associated with tumor growth and metastasis. Bacteriostasis can not only inhibit the growth and metastasis of tumors during their early diagnosis and treatment but also prevent bacterial infections after recovery. In this study, we synthesized Ag@GQD which consisted of nitrogen-doped graphene quantum dots (N-GQD) wrapped around Ag NPs through the photocatalytic reduction method. Multifunctional nanoprobes (Ag/DOX@GQD) were obtained by doxorubicin (DOX) loading. Ag@GQD exhibited high stability, low cytotoxicity, and good photoacoustic imaging performance. The effective photothermal performance of Ag@GQD enabled us to achieve the controlled release of DOX from probes using near-infrared light and pH response during the treatment of tumors using chemo-photothermal combination therapy. Results from cell-based experiments revealed that combination therapy was superior to single-drug therapy. Besides, Ag@GQD could effectively kill Staphylococcus aureus and Escherichia coli and can be expected to inhibit the growth and metastasis of tumors caused by bacteria and in reducing bacterial infections after recovery.
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CURRENT MICROBIOLOGY
·
2022
·Vol. 79
·期 6
·1-11
·
IF 2.343
Bao Jingjiao,Guo Dengkang,Jin Lei,Li Tiejun,Shi Hui
Vibrio fluvialis is a marine opportunistic pathogen that frequently causes diseases in aquatic animals and humans. V. fluvialis can produce quorum sensing signaling molecules to coordinate cell density-dependent behavioral changes, including N-acyl homoserine lactone (AHL), which acts as a vital mediator of virulence-associated gene expression. Currently, several AHL molecules in V. fluvialis have been detected via biological and physicochemical methods, although different detection approaches have generated diverse AHL profiles. Here, we describe the AHL-producing bacterium, V. fluvialis BJ-1, which was isolated from marine sediments from the East China Sea. V. fluvialis BJ-1 could stimulate AHL-mediated β-galactosidase synthesis of the biosensor Agrobacterium tumefaciens NTL4 (pZLR4) but could not induce violacein production in the AHL reporter strain, Chromobacterium violaceum CV026. This bacterial isolate exhibited strong AHL-producing activity at low cell density; however, the AHL activity declined when population density remained at high levels. Analysis of the AHLs by Ultra-High-Performance Liquid Chromatography tandem Mass Spectrometry demonstrated that V. fluvialis BJ-1 produced five different AHL signaling molecules, including two linear chain AHL products (C 8 - and C 10 -HSL), and three β-carbon-oxidative AHL products (3-O-C 8 -, 3-O-C 10 - and 3-O-C 12 -HSL). Significantly, the present study is the first to accurately define the AHL profile of marine V. fluvialis . In future, the coupling of UHPLC to ESI–MS/MS is expected to be utilized for the accurate determination of AHL profiles in marine Vibrio .
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NATURE
·
2022
·Vol. 605
·期 7908
·69-75
·
IF 69.504
Liu Lei,Li Taotao,Ma Liang,Li Weisheng,Gao Si,Sun Wenjie,Dong Ruikang,Zou Xilu,Fan Dongxu,Shao Liangwei,Gu Chenyi,Dai Ningxuan,Yu Zhihao,Chen Xiaoqing,Tu Xuecou,Nie Yuefeng,Wang Peng,Wang Jinlan,Shi Yi,Wang Xinran
Two-dimensional transition-metal dichalcogenides (TMDs) are of interest for beyond-silicon electronics 1 , 2 . It has been suggested that bilayer TMDs, which combine good electrostatic control, smaller bandgap and higher mobility than monolayers, could potentially provide improvements in the energy-delay product of transistors 3 , 4 , 5 . However, despite advances in the growth of monolayer TMDs 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , the controlled epitaxial growth of multilayers remains a challenge 15 . Here we report the uniform nucleation (>99%) of bilayer molybdenum disulfide (MoS 2 ) on c -plane sapphire. In particular, we engineer the atomic terrace height on c -plane sapphire to enable an edge-nucleation mechanism and the coalescence of MoS 2 domains into continuous, centimetre-scale films. Fabricated field-effect transistor (FET) devices based on bilayer MoS 2 channels show substantial improvements in mobility (up to 122.6 cm 2 V −1 s −1 ) and variation compared with FETs based on monolayer films. Furthermore, short-channel FETs exhibit an on-state current of 1.27 mA μm −1 , which exceeds the 2028 roadmap target for high-performance FETs 16 .
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高影响因子
危险品化学品经营许可证（带存储）