JOURNAL OF CHROMATOGRAPHY A
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2024
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IF 3.8
Jiana Lin,Xiaoyan Ouyang,Yuling Hu,Gongke Li,Qisheng Zhong
Acurate and sensitive determination of hazards from food contact materials is important to monitor food safety. It is necessary to excavate efficient adsorbent for simultaneous recognition and adsorption of food hazards of trace level for sample preparation. In this work, β-cyclodextrin and calix[4]arene were employed as hybrid functional monomers to prepare macrocyclic porous organic polymer (β-CD-CX4 POP). It was proved that the supramolecular cavities of β-CD-CX4 POP could form inclusion complexes with fluorescent whitening agents (FWAs) through host-guest recognition, which greatly improved the adsorption performance. The hydrophobic cavities of β-cyclodextrin and calix[4]arene of β-CD-CX4 POP exhibited synergistic effect for simultaneous recognition of FWAs. The high-throughput enrichment of FWAs was realized by β-CD-CX4 POP membranes coupled with a multiple-channel syringe pump. Based on membrane-based solid-phase extraction combined with UHPLC-MS/MS, a sensitive analytical method was established to determine six FWAs. The LODs was in range of 3-50 ng/L with the linear range of 0.02-100 μg/L. The developed method was used to quantify FWAs in bread wrapper and bread, and the spiked recoveries ranged from 78.1%-119% with RSD of 2.3%-9.7%. This work indicated that β-CD-CX4 POP was promising for the simultaneous recognition and adsorption of FWAs migrating from food contact materials.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Mshari A. Alotaibi,Awais Khalid,Abdulrahman I. Alharthi
In this study, β-cyclodextrin-modified cobalt oxide (Co 3 O 4 @β-CD) composites were developed through hydrothermal processes and employed for heterogeneous activation of peroxymonosulfate (PMS) for the removal of ciprofloxacin (CIP) antibiotics. The results revealed that the degradation of CIP by Co 3 O 4 @β-CD/PMS was 99.13 %, which was significantly higher than the degradation of CIP by Co 3 O 4 /PMS (79.06 %) in a 25-minute period under optimal conditions ([Co 3 O 4 @β-CD] = 0.25 g/L, [PMS] = 0.25 mM, pH=7.0). The enhanced degradation of CIP contributed to the greater surface area (162.84 m 2 /g) of the Co 3 O 4 @β-CD to the Co 3 O 4 (121.47 m 2 /g). Besides, the study assessed the impact of several reaction variables and interrupting anions on the abatement of CIP. A possible degradation procedure for CIP has been established by identifying reaction intermediates. The activation of PMS was achieved by both radical and non-radical routes. Further, Co 3 O 4 @β-CD exhibited remarkable stability and maintained its degrading efficiency after five consecutive cycles.
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PHYTOTHERAPY RESEARCH
·
2025
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IF 6.3
Qian Hu,Mengyao Wang,Meng Chen,Jinjin Wang,Ting Niu
Diffuse large B-cell lymphoma (DLBCL) is an aggressive and heterogeneous subtype of non-Hodgkin lymphoma, with two-thirds of patients relapsing or resisting existing therapies, highlighting the urgent need for effective treatments. Toosendanin (TSN), a triterpenoid from Meliae Cortex, exhibits significant anti-cancer activity by modulating cell survival and proliferation. This study investigates the anti-lymphoma effects and underlying mechanisms of TSN, proposing it as a potential therapeutic agent to address the challenges of DLBCL. Network pharmacology, molecular docking, and transcriptome sequencing were employed to predict TSN's anti-DLBCL potential. Findings were validated through in vitro and in vivo experiments, including cell viability assays, flow cytometry, quantitative PCR, Western blotting, reverse experiments with small-molecule inhibitors or genetic editing, and a cell-derived xenograft (CDX) model. Bioinformatics analyses revealed TSN's strong binding affinity to PI3Kα/β and Polo-like kinase 1 (PLK1). Experiments showed that TSN downregulated the PI3K/Akt signaling pathway and reduced PLK1 mRNA and protein levels, inducing apoptosis, cell cycle arrest, and cell death in DLBCL cells. RNA sequencing and metabolic assays indicated TSN upregulated cholesterol biosynthesis in DLBCL cells. Co-treatment with a statin enhanced TSN's anti-DLBCL effects while mitigating hepatic and pulmonary toxicity. This study identifies TSN as a dual inhibitor of PI3K and PLK1 with significant therapeutic potential for DLBCL. It also proposes a lipid-modulating strategy to enhance TSN's cytotoxicity while reducing adverse effects, offering a promising approach to improve DLBCL treatment outcomes.
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Biosensors-Basel
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2024
·Vol. 14
·期 4
·169
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IF 4.9
Xinyi Fan,Yuexin Bao,Yanhong Chen,Xiaohong Wang,Stephen L. W. On,Jia Wang
The peroxidase-like behaviors of gold nanoparticles (AuNPs) have the potential to the development of rapid and sensitive colorimetric assays for specific food ingredients and contaminants. Here, using NaBH4as a reducing agent, AuNPs with a supramolecular macrocyclic compound β-cyclodextrin (β-CD) capped were synthesized under alkaline conditions. Monodispersal of β-CD@AuNPs possessed a reduction in diameter size and performed great peroxidase-like activities toward both substrates, H2O2and TMB. In the presence of H2O2, the color change of TMB oxidization to oxTMB was well-achieved using β-CD@AuNPs as the catalyst, which was further employed to develop colorimetric assays for ascorbic acid, with a limit of detection as low as 0.2 μM in ddH2O. With the help of the host-guest interaction between β-CD and adamantane, AuNPs conjugated with nanobodies to exhibit peroxidase-like activities and specific recognition againstSalmonellaTyphimurium simultaneously. Based on this bifunctional bioprobe, a selective and sensitive one-step colorimetric assay forS. Typhimurium was developed with a linear detection from 8.3 × 104to 2.6 × 108CFU/mL and can be provided to spiked lettuce with acceptable recoveries of 97.31% to 103.29%. The results demonstrated that the excellent peroxidase-like behaviors of β-CD@AuNPs can be applied to develop a colorimetric sensing platform in the food industry.
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Xinrui Liu,Yupeng Li,Dairong Chen,Xiuling Jiao,Yuguo Xia
The selective hydrogenation of α,β-unsaturated aldehydes to unsaturated alcohols is a cornerstone of fine chemical synthesis, yet it remains a formidable challenge due to competing hydrogenation of thermodynamically favored C C bonds over kinetically sluggish C O bonds. Here, we present a PtFe dual-atom catalyst anchored on defective polymeric carbon nitride (DPCN), which achieves nearly complete conversion and exceptional selectivity toward cinnamyl alcohol (COL) under mild conditions. Particularly, the PtFe/DPCN exhibits a superior turnover frequency and achieves 99.02 % conversion of cinnamaldehyde (CAL) with 99.34 % selectivity for COL at 70 °C and 2 MPa H 2 . Kinetic studies and in-situ DRIFTS analyses reveal that this dual-atom architecture facilitates preferential C O adsorption and hydrogenation while suppressing C C activation. In addition, DFT calculations further show that the Pt-Fe dual atomic pair lowers the activation barrier by modulating the d -band center and Gibbs free energy profiles, enhancing H 2 splitting and intermediate stabilization. This work demonstrates a promising strategy for fine-tuning electronic and geometric structures via dual-atom design, offering an effective route for developing highly selective hydrogenation catalysts under industrially viable conditions.
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World Journal of Stem Cells
·
2025
·Vol. 17
·期 7
·104607
·
IF 3.6
Kun Wang,Wei Lin,Jun-Yong Han,Jin-Yan Chen,Rong-Hua Liu,Zhen Yu,Jing-Jun Jin
BACKGROUND Type 1 diabetes (T1D) results from the autoimmune-mediated loss of pancreatic β-cells. Current insulin therapies offer symptomatic relief but fall short of providing a definitive cure. Islet cell transplantation, while promising, faces limitations related to donor scarcity, procedural complexities, and the necessity for long-term immunosuppression. Consequently, there is an urgent need for innovative strategies aimed at β-cell regeneration. Patient-derived induced pluripotent stem cells (iPSCs), obtained from peripheral blood mononuclear cells (PBMCs) of T1D patients, hold great potential as a source of cells for therapeutic purposes. Therefore, the differentiation of T1D-iPSCs into functional pancreatic β-cells is a critical step toward effective β-cell replacement therapy. AIM To assess the potential of patient-derived T1D-β-like cells (differentiated from T1D-iPSCs reprogrammed from T1D-PBMCs) for restoring β-cell function in T1D. METHODS T1D-iPSCs were reprogrammed from T1D-PBMCs using an episomal vector-based approach. Pluripotency was confirmed by flow cytometry (FCM), quantitative real-time polymerase chain reaction, genomic stability analysis, and teratoma formation assays. Differentiation into pancreatic β-cells was optimized using triiodothyronine (T3), vitamin C (Vc), and an adenovirus (M3C) encoding pancreatic duodenal homeobox-1, neurogenin 3 ( Ngn3 ), and MAF bZIP transcription factor A ( MafA ). Following characterization of β-cell features by immunofluorescence, quantitative real-time polymerase chain reaction, and flow cytometry, therapeutic efficacy was assessed through blood glucose monitoring after transplantation under the renal capsule of streptozotocin-induced diabetic mice. RESULTS T1D-iPSCs were successfully generated from T1D-PBMCs. These cells exhibited the hallmark characteristics of pluripotent stem cells, including appropriate morphology, differentiation potential, genomic integrity, and expression of pluripotency-associated genes. Differentiation yielded insulin-positive (insulin + ) pancreatic β-like cells that, at the mRNA level, expressed key β-cell markers such as pancreatic duodenal homeobox-1, Ngn3 , MafA , NeuroD , glucagon-like peptide-1 receptor, Nkx6.1 , glucose transporter 2, and Kir6.2 . Notably, the T3 + Vc group displayed the lowest Ngn3 expression (1.31 ± 0.38 vs 1.96 ± 0.25 vs 2.51 ± 0.24, P < 0.01), while the M3C + T3 + Vc group exhibited the highest MafA expression (0.49 ± 0.11 vs 0.32 ± 0.06 vs 0.29 ± 0.08, P < 0.05). Both in vitro and in vivo assessments confirmed the insulin secretion ability of the generated β-like cells; however, they did not demonstrate appropriate modulation of insulin release in response to variations in extracellular glucose concentrations. CONCLUSION T1D-iPSCs derived from T1D-PBMCs can be differentiated into insulin + β-like cells, albeit with functional immaturity. These cells represent a potential source of seed cells for β-cell replacement therapy in T1D.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Si Shi,Junjie Tang,Yanyan Zhou,Hongyan Huang,Jianxi Zhang,Wenhao Liu,Zhiwen Hu,Hongwei Wang,Jie Pang,Chunhua Wu
Maintaining the safety and quality of minimally processed agricultural products is a significant challenge in modern food supply chains. This study developed multifunctional sodium alginate (SA)-based films incorporating high internal phase Pickering emulsions (HIPPEs) stabilized by curcumin/β-cyclodextrin inclusion complexes (CUR/β-CD). The HIPPEs demonstrated excellent colloidal stability (>21 days) with a mean droplet diameter of 12.76 ± 0.32 μm. Through solvent casting, HIPPEs were uniformly integrated into SA matrices (0.5–2 % w/w). Based on a comprehensive characterization of the films, the optimum concentration of 1.5 % for doping with HIPPEs was determined. The SA/HIPPEs1.5 films exhibited excellent tensile strength (14.21 MPa), elongation at break (26.28 %), and adequate water vapor (6.5 × 10 −11 g/(m·s·Pa)) and oxygen permeability (1.06 × 10 −3 g/m 2 ·s). Furthermore, they demonstrated dual antimicrobial-antioxidant functionality, with inhibition zones of 19.30 ± 0.70 mm ( Staphylococcus aureus ) and 20.53 ± 0.40 mm ( Escherichia coli ), along with 70.63 % DPPH radical scavenging activity. Compared to polyethylene packaging, SA/HIPPEs1.5 films effectively preserved blueberry quality at 25 °C for 18 days, showing lower weight loss (30.6 % vs. 42.2 %) and a favorable pH (3.43 vs. 4.22). These findings support the potential of SA/HIPPEs composite films as an effective active packaging solution for extending blueberry shelf life.
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BIOELECTROCHEMISTRY
·
2025
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IF 4.5
Xue Kong,Zimei Yu,Qinghua Sun,Yan Liu,Zheng-Zhi Yin
Monitoring beta-amyloid 1 – 42 (Aβ 42 ) is vital and challenging, which is a typical biomarker of Alzheimer's disease. Here, a novel electrochemical microbiosensor is developed to detect Aβ 42 on an acupuncture needle. Hemin is well known for its characteristics, including its ability to self-assemble on single-walled carbon nanotube (SWCNT), the molecular interaction with Aβ 42 , and the intrinsic electroactive signal. These properties are exploited to anchor and respond to Aβ 42 after integrating a molecularly imprinted surface polymer (SMIP). The SMIP layer of polydopamine/poly (ionic liquid) can be prepared by electropolymerization on an acupuncture needle microelectrode (ANME), which undergoes growth and formation of a polymeric structure around the anchored Aβ 42 . Interestingly, the imprinted cavities express a fluent signal of built-in hemin after eluting the templates, and show a highly selective and sensitive hindrance response for the recombined Aβ 42 . Under optimized conditions, the microbiosensor displays a linear range of 100 to 1 × 10 10 fM with a limit of detection of 0.05 fM. There are development and advances for the discipline of electroanalysis after comparing the technique and important indicators with the electrochemical biosensors reported of Aβ 42 . The microbiosensor also exhibited excellent selectivity, good stability, and reproducibility, which was effectively used to detect Aβ 42 in real spiked samples. The improved behavior of the developed microbiosensor can be attributed to its superficial highly matched imprinted cavities, built-in hemin label, and electronic barrier without signal of the nonimprinted surface to outside molecules. This microbiosensor has a scientific and reference value for directly sensing non-electroactive biomarkers, functionalizing microelectrodes, and electron transport cavities. It would also be amazing if this new microbiosensor could combine with the unclear and magical property of acupuncture in the treatment of neurological disorders.
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Aggregate
·
2024
·
IF 13.9
Manyu Zhu,Shanshan Li,Qixin Liu,Yuqi Zhang,Zihao Li,Yiran Wang,Lie Wu,Xiue Jiang
Surface chirality plays an important role in determining the biological effect, but the molecular nature beyond stereoselectivity is still unknown. Herein, through surface-enhanced infrared absorption spectroscopy, electrochemistry, and theoretical simulations, we found diasteromeric monolayers induced by assembled density on chiral gold nanofilm and identified the positive contribution of water dipole potential at chiral interface and their different interfacial interactions, which result in a difference both in the positive dipoles of interfacial water compensating the negative surface potential of the SAM and in the hindrance effect of interface dehydration, thereby regulating the interaction between amyloid-β peptide (Aβ) and N -isobutyryl-cysteine (NIBC). Water on L-NIBC interface which shows stronger positive dipole potential weakens the negative surface potential, but its local weak binding to the isopropyl group facilitates hydrophobic interaction between Aβ42 and L-NIBC and resulted fiber aggregate. Conversely, electrostatic interaction between Aβ42 and D-NIBC induces spherical oligomer. These findings provide new insight into molecular nature of chirality-regulated biological effect.
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SENSORS
·
2024
·Vol. 24
·期 4
·1093
·
IF 3.4
Zhen Li,Yang Hu,Liu Wang,Houfang Liu,Tianling Ren,Cong Wang,Daoliang Li
A surface-enhanced Raman scattering (SERS) method for measuring nitrate nitrogen in aquaculture water was developed using a substrate of β-cyclodextrin-modified gold nanoparticles (SH-β-CD@AuNPs). Addressing the issues of low sensitivity, narrow linear range, and relatively poor selectivity of single metal nanoparticles in the SERS detection of nitrate nitrogen, we combined metal nanoparticles with cyclodextrin supramolecular compounds to prepare a AuNPs substrate enveloped by cyclodextrin, which exhibits ultra-high selectivity and Raman activity. Subsequently, vanadium(III) chloride was used to convert nitrate ions into nitrite ions. The adsorption mechanism between the reaction product benzotriazole (BTAH) of o-phenylenediamine (OPD) and nitrite ions on the SH-β-CD@AuNPs substrate was studied through SERS, achieving the simultaneous detection of nitrate nitrogen and nitrite nitrogen. The experimental results show that BTAH exhibits distinct SERS characteristic peaks at 1168, 1240, 1375, and 1600 cm−1, with the lowest detection limits of 3.33 × 10−2, 5.84 × 10−2, 2.40 × 10−2, and 1.05 × 10−2μmol/L, respectively, and a linear range of 0.1–30.0 μmol/L. The proposed method provides an effective tool for the selective and accurate online detection of nitrite and nitrate nitrogen in aquaculture water.
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Natural Products and Bioprospecting
·
2024
·Vol. 14
·期 1
·1-16
·
IF 4.8
Yang Ya-Ya,Jing Shun-Li,Shao Jia-Li,Chen Ji-Xuan,Zhang Wei-Feng,Wan Si-Yuan,Shen Yu-Ping,Yang Huan,Yu Wei
Functionalized mesoporous materials have become a promising carrier for enzyme immobilization. In this study, Santa Barbara Amorphous 15 (SBA-15) was modified by N-aminoethyl- γ -aminopropyl trimethoxy (R). R-SBA-15 was employed to purify and immobilize recombinant β -glucosidase from Terrabacter ginsenosidimutans (BgpA) in one step for the first time. Optimum pH of the constructed R-SBA-15@BgpA were 7.0, and it has 20 ℃ higher optimal temperature than free enzyme. Relative activity of R-SBA-15@BgpA still retained > 70% at 42 ℃ after 8-h incubation. The investigation on organic reagent resistance revealed that the immobilized enzyme can maintain strong stability in 15% DMSO. In leaching test and evaluation of storage stability, only trace amount of protein was detected in buffer of the immobilized enzyme after storage at 4 ℃ for 33 days, and the immobilized BgpA still maintained > 50% relative activity. It also demonstrated good reusability, with 76.1% relative activity remaining after fourteen successive enzymatic hydrolyses of epimedin A to sagittatoside A. The newly proposed strategy is an effective approach for the purification and immobilization of BgpA concurrently. In addition, R-SBA-15@BgpA was demonstrated to have high efficiency and stability in this application, suggesting its great feasibility and potential to produce bioactive compounds such as secondary glycosides or aglycones from natural products. Graphical Abstract
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Regenerative Engineering and Translational Medicine
·
2025
·
IF 1.9
Pradana F. Josse Pasca,Handayani Salsabila Dwi,Phalosa Ghani,Salsabila Mirza,Wicaksono Ikhsan Adil,Al-Ghifary M. Raafi,Putri Dita Sheilla,Arsyadi,Tuba Syahrul
Background Uncontrolled hemorrhage is a leading cause of preventable mortality in trauma cases, necessitating rapid and effective hemostatic interventions. Conventional methods such as gauze dressings and tourniquets have limitations, including delayed clot formation, bacterial contamination, and potential tissue damage. Recent advancements in biomaterials have highlighted chitosan/β-glycerophosphate (CS/β-GP) hydrogels as promising thermosensitive agents for emergency hemostasis. Objective This literature review explores the potential of chitosan/β-glycerophosphate (CS/β-GP) thermosensitive aerosol hydrogel as a novel hemostatic agent, emphasizing its physicochemical properties, gelation mechanisms, delivery innovations, and strategies to enhance its mechanical strength. Methods Relevant scientific literature was collected from Scopus, PubMed, CrossRef, and Semantic Scholar databases using targeted
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危险品化学品经营许可证（带存储）