Acta Biomaterialia
·
2024
·
IF 9.4
Yan Ju,Shiyuan Ma,Meimei Fu,Min Wu,Yue Li,Yue Wang,Meihan Tao,Zhihui Lu,Jinshan Guo
Discectomy is the surgical standard of care to relieve low back pain caused by intervertebral disc (IVD) herniation. However, there remains annulus fibrosus (AF) defect and nucleus pulposus (NP) degeneration, which often result in recurrent herniation (re-herniation). Herein, we develop a polyphenol-modified waterborne polyurethane bioadhesives (PPU-glues) to promote therapy prognosis after discectomy. Being composed of tannic acid (TA) mixed cationic waterborne polyurethane nanodispersions (TA/WPU + ) and curcumin (Cur) embedded anionic waterborne polyurethane nanodispersions (Cur-WPU - ), PPU-glue gels rapidly (<10 s) and exhibits low swelling ratios, tunable degradation rates and good biocompatibility. Due to the application of an adhesion strategy combing English ivy mechanism and particle packing theory, PPU-glue also shows considerable lap shear strength against wet porcine skin (≈58 kPa) and burst pressure (≈26 kPa). The mismatched particle sizes and the opposite charges of TA/WPU + and Cur-WPU - in PPU-glue bring electrostatic interaction and enhance particle packing density. PPU-glue possesses superior reactive oxygen species (ROS)-scavenging capacity derived from polyphenols. PPU-glue can regulate extracellular matrix (ECM) metabolism in degenerated NP cells, and it can promote therapy biologically and mechanically in degenerated rat caudal discs. In summary, this study highlights the therapeutic approach that combines AF seal and NP augmentation, and PPU-glue holds great application potentials for post discectomy therapy. Statement of Significance Currently, there is no established method for the therapy of annulus fibrosus (AF) defect and nucleus pulposus (NP) degeneration after discectomy. Herein, we developed a polyphenol-modified biomimetic polyurethane bioadhesives (PPU-glue) with strong adhesive strength and superior bioactive property. The adhesion strategy that combined a particle packing theory and an English ivy mechanism was firstly applied to the intervertebral disc repair field, which benefited AF seal. The modified method of incorporating polyphenols was utilized to confer with ROS-scavenging capacity, ECM metabolism regulation ability and anti-inflammatory property, which promoted NP augmentation. Thus, PPU-glue attained the synergy effect for post discectomy therapy, and the design principle could be universally expanded to the bioadhesives for other surgical uses.
研究领域:
生物医学工程
·
组织工程
·
骨科
·
材料科学
✓ 已验证
DOI
高影响因子
FOOD MICROBIOLOGY
·
2021
·
IF 5.516
Shiqi Li,Pengfei Bi,Nan Sun,Zhiyi Gao,Xiaowen Chen,Jing Guo
To improve the functional property and flavor quality of kiwi wine, the performance of 11 strains of non- Saccharomyces yeasts from 5 species were comprehensively characterized in kiwi wine. Chemical compositions and sensorial profiles of all kiwi wines were assessed. The results indicated that most non- Saccharomyces cerevisiae produced more polyphenols than Saccharomyces cerevisiae WLS21 (Sc21). A total of 130 volatiles were observed in the kiwi wines. Zygosaccharomyces rouxii IFO30 (Zr30), Zygosaccharomyces bailii IFO37 (Zb37) and Schizosaccharomyces pombe 1757 (Sp57) were found to produce more concentration of volatile compounds than the other strains including Sc21. 25 volatiles with a rOAV ≥0.1 were identified. Principal component analysis (PCA) revealed that Zr30 and Zb37 specifically increased the concentrations of ethyl esters, 2-methylbutan-1-ol and phenethyl acetate, while Sp57 primarily enhanced the contents of phenylacetaldehyde, 2-methylbutan-1-ol and phenethyl acetate. The sensory analysis demonstrated that Zr30 and Zb37 strains were more optimal than S. cerevisiae in aroma generation. In addition, the partial least-squares regression (PLSR) analysis revealed that tropical fruits, red fruits, dried fruits, flowers and floral odors showed an intensely positive impact on the overall acceptability of the kiwi wine.
研究领域:
食品科学
·
微生物学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Mingyan Jia,Yixuan Pan,Jianbo Zhou,Mei Zhang
In this work, we developed an optical colorimetric sensor array for the discrimination of Chinese teas. The sensor array was carefully designed based on tea polyphenol induced indicators displacement assay (IDA), using phenylboronic acids with different substituents as the receptors to polyphenols. The accurate identification for polyphenols with different species or concentrations proved the potential of the sensor array. The sensor array successfully distinguished tea samples within different categories, grades and origins, coupling with PLS-DA. This work offered an efficient and rapid method to distinguish teas and tea-related products. Besides, the assay is supposed to be suitable for the identification of other polyphenol-related natural products.
研究领域:
食品科学
·
分析化学
✓ 已验证
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2022
·
IF 9.224
Wen Tang,Haoxiang Li,Wei Zhang,Tonghao Ma,Jiafeng Zhuang,Ping Wang,Chao Chen
Site-specific and covalent attachment is the most desirable immobilization strategy, but classic methods typically require genetic engineering or complicated material fabrication, resulting in operational complexity and difficulty. Herein, a novel site-specific and covalent immobilization strategy based on accurately selected immobilization sites on lipase was developed. Specifically, computer-aided structural analysis of functional groups on lipase revealed that lysine residues with free amino groups were far away from the catalytic pocket and lid, which were suitable to be chosen as the best immobilization sites to effectively reduce the loss in its activity. Meanwhile, natural polyphenol-modified magnetic nanoparticles could increase the active immobilization sites, and lipase can be immobilized on them directly via a covalent reaction. This site-specific immobilization system exhibited significant enhancement in activity recovery (71.3%) compared to random immobilization (42.5 and 55.9%). As expected, experimental and computational analyses revealed that tailor-made site-specific immobilization carriers were beneficial to maintain the native catalytic pocket conformation and enhance the rigidity of the immobilized lipase, which exhibited a higher biodiesel yield (92.1%) than free and randomly immobilized lipases. Besides, the site-specifically immobilized lipase could maintain as high as 75.3% biodiesel yield after eight cycles, making it an ideal nanocatalyst for efficient production of biodiesel. Overall, the site-specific enzyme immobilization technology can provide stable catalytic activity advantages over the randomly covalent immobilization strategy, which can significantly promote green manufacturing and sustainable production.
研究领域:
生物化学
·
纳米技术
·
可再生能源
✓ 已验证
DOI
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 48
·28746-28754
·
IF 3.119
Yuan Ma,Ming Luo,Yingping Xu,Yingjia Liu,Xiaocui Liu,Xiufang Bi,Yiping Yuan,Fan Su,Xiaocui Yin
Thaumatin-like protein-1 (TLP-1), a protein displaying high polyphenol oxidase (PPO) action and a member of the pathogenesis-related (PR) protein family, has a considerable influence on the enzymatic browning of Prunus mume (Chinese plum). In this assay, TLP-1 was identified and extracted from Prunus mume to investigate the protein's properties and better understand its contribution to the fruit's browning during storage or processing. The extracted TLP-1 was purified to apparent homogeneity using a procedure involving citrate phosphate buffer solution (CPBS) extraction, (NH4)2SO4 precipitation, dialysis in a cellulose bag, and ion exchange chromatography using a DEAE Sepharose Fast Flow column, while a Sephadex G-75 column was employed to facilitate gel filtration chromatography. Moreover, the enzyme was characterized in terms of its optimal pH and stability, isoelectric point (pI), molecular weight, optimal temperature and stability, enzyme kinetic parameters and substrate specificity, as well as inhibitor stability. This study indicated that the pI and molecular weight of TLP-1 was approximately 4.4 and 28 kDa, respectively, while 30 °C and 7.5 represented the respective optimal temperature and pH level for PPO catalysis. TLP-1 showed high affinity to catechol and pyrogallol, with Km values of 24.40 mM and 26.23 mM, respectively. Sodium bisulfite significantly inhibited TLP-1 activity. These findings on the properties of TLP-1 can contribute significantly to the search for ways to minimize the losses caused by fruit browning during the storage and processing of Prunus mume.
研究领域:
植物科学
·
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
International Journal of Pharmaceutics-X
·
2026
·
IF 7.9
Yinghong Huang,Yuanyuan Zhao,Yuhong Su,Chudong He,Mengdie Mo,Weijie Zhou,Yang Lu,Fei Yu
Ferroptosis, an iron-dependent programmed cell death process driven by reactive oxygen species (ROS) accumulation, represents a promising therapeutic strategy for breast cancer. However, the efficacy of ferroptosis therapy in breast cancer is often compromised by insufficient intracellular levels of hydrogen peroxide (H 2 O 2 ) and iron ions. To address these problems, we developed pH-responsive nanoparticles comprising a metal–phenolic network (MPN) shell and a bovine serum albumin (BSA) core for the delivery of artesunate (ART) (designated as BAM NPs). The designed BAM NPs aim to amplify oxidative stress and enhance ferroptosis-based therapy for breast cancer. Upon endocytosis by tumor cells, BAM NPs underwent degradation under acidic conditions to release ART and Fe 3+ . Subsequently, the reduction of Fe 3+ to Fe 2+ by glutathione (GSH) initiated the Fenton reaction, which led to aberrant accumulation of ROS. Meanwhile, the endoperoxide bridge of ART could be cleaved by Fe 2+ to further generate carbon-centered radicals (·C). Furthermore, BAM NPs effectively deplete GSH via Fe 3+ /Fe 2+ conversion to inactivate glutathione peroxidase 4 (GPX4), thereby disrupting redox homeostasis and increasing intracellular LPO levels. Both in vitro and in vivo experiments showed that BAM NPs significantly inhibited tumor cell proliferation by inducing robust ferroptosis in tumor cells, leading to a tumor inhibition rate of 83.11%. In summary, the constructed BAM NPs served as a promising tailored nanoplatform for augmenting ferroptosis therapy in breast cancer.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Jianning Yu,Enfeng Yang,Haitao Deng,Guoliang Liu,Wenxuan Zhao,Xiangdong Yang,Xiugong He,Dezhao Hao,Ye Tian,Lei Jiang
The growth in oil demand has driven technological innovation in enhanced oil recovery (EOR). Chemical flooding, widely adopted for its effectiveness in improving oil recovery, enhances the macroscopic sweep efficiency by altering interfacial properties. However, strong oil–solid adhesion leads to residual oil, posing a key technical challenge. Although low-salinity water flooding can disrupt the hydrated ion bridge structure, its efficacy is limited, and it fails to fully resolve residual oil issues. To tackle this residual oil challenge, we developed traditional Chinese medicine (TCM) composite agents based on polyphenol–silicon complexation with dual EOR functions: in situ oil displacement and long-term oil isolation. Under weak alkaline conditions, the TCM composite agent continuously reacts with silicon, overcoming van der Waals forces between crude oil and silicate, thereby effectively displacing oil in micron-scale grooves. The polyphenol–silicon complexes, along with polyphenol, form a hydrophilic coating with micro/nanostructures via π–π interactions, maintaining superoleophobic and nonadhesive properties against crude oil even after 1 week. Additionally, the TCM agents altered the wettability of shale from oil-wet to water-wet and achieved an 88.24% recovery rate in core flooding tests. This breakthrough offers a solution for crude oil recovery, promoting efficient and environmentally sustainable development in the industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Science Advances
·
2026
·Vol. 12
·期 13
·
IF 13.9
Taisong Fang,Songbai Liu
Efficient oral delivery of RNA to the target site is a long-standing issue for nucleic acid–based therapy. Herein, we adopted a vesicle-to-vesicle transfer strategy and established an efficient approach to encapsulate and stabilize RNA for targeted oral delivery. The amphiphilic specifically acylated epigallocatechin directly performed encapsulation of RNA and generated nanovesicles in high efficiency without assistance of additional materials. The RNA encapsulated in the nanovesicles was efficiently transferred to outer membrane vesicles (OMVs) derived from Escherichia coli Nissle 1917 probiotic through membrane fusion with simple operation. The derived hybrid vesicles (HVs) were further anchored with bilirubin and Lys-Asp-Glu-Leu grafted hyaluronic acid (HA-BR-KDEL) ligand for sequential cellular and intracellular targeting to the endoplasmic reticulum of inflammatory cells in inflamed intestinal tract. Oral delivery of HVs@HA-BR-KDEL notably alleviated colitis symptoms in mice and contributed to the restoration of intestinal homeostasis. The tea polyphenol hybrid OMV strategy holds great promise for oral gene-mediated treatment.
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2026
·
IF 2.6
Wen Su,Tao Yao,Fen Ran
ZIF-8 have been extensively studied in water treatment membranes, and they exhibit excellent modification effects in the membranes. In this study, tea polyphenol-coated ZIF-8 nanoparticles are synthesized via one-pot synthesis and then are blended to modify polyethersulfone ultrafiltration membrane. The shape of the nanoparticles is affected by changing the content of tea polyphenol. Different nanoparticles blends polyethersulfone for ultrafiltration membranes. It is found that adding ZIF-8@Tea polyphenol with hydrophilic properties could significantly improve wettability of membrane; when 0.3 g of ZIF-8@Tea polyphenol is added, the film contact angle decreases to 27.3 ° and shows an excellent rejection rate for methylene blue, methyl orange, and Congo red. The addition of ZIF-8 significantly enhances the retention rate of bovine serum albumin by water treatment membranes, which is mainly attributed to its precise size sieving effect. The accumulation or growth of ZIF-8 particles creates more uniform nanoscale channels, ultimately reducing the average and maximum pore diameters. The effective separation layer of membranes make much smaller than the size of bovine serum albumin. The strategy not only enables the ultrafiltration membrane to maintain high water flux under different pressures but also endows it with the ability to resist Escherichia coli microorganisms.
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2026
·
IF 11
Xueqiang Deng,Zhiqiang Li,Xuan Yi,Dong Ruan,Xiang Chen,Jun Luo
Osteoporosis poses a significant threat to human health and severely impairs patients' quality of life. Conventional pharmacological interventions are limited by poor patient compliance and suboptimal efficacy. Imbalanced macrophage polarization is a key driver in osteoporosis pathogenesis, and precise regulation toward the anti-inflammatory M2 phenotype represents a critical therapeutic target. A calcium-polyphenol network-functionalized black phosphorus nanosheet system (HCBP) is developed as a bone-targeted cascade-responsive nanoplatform. After intravenous administration, bone-targeting peptides mediate efficient accumulation in bone tissue. The system dissociates in the acidic osteoporotic microenvironment and releases phosphate ions, which combine with calcium ions to form bioactive calcium phosphate. HCBP alleviates inflammation, sustains M2 macrophage polarization, scavenges reactive oxygen species, enhances osteogenesis, and suppresses osteoclast differentiation. Transcriptomic analysis confirms regulation of the TNF-α/NF-κB, IL-17, and osteoclast differentiation pathways, together with inhibited glycolysis. This multifunctional strategy overcomes the limitations of single-target osteoporosis therapies and facilitates the clinical translation of nanomedicines for metabolic bone diseases.
✓ 已验证
产品关联
DOI
高影响因子
Small
·
2026
·
IF 11.8
Tingxu Guo,Yawen Huang,Wanmeng Liu,Shaojian Lin,Jianwu Lan,Jiaojiao Shang
The design of universal nanocarriers with broad-spectrum drug compatibility, high loading efficiency, and controlled release remains a significant challenge in drug delivery. Herein, a versatile platform based on black phosphorus nanosheets modified with a metal–polyphenol network (BP@MPN) is presented. The MPN coating significantly enhances the structural stability of BP and introduces a multifunctional interface capable of diverse non-covalent interactions, including hydrogen bonding, electrostatic, hydrophobic, and π interactions, to support the efficient loading of a wide range of therapeutic agents. A multidimensional evaluation demonstrates that BP@MPN exhibits superior loading performance across small molecules, proteins, inorganic nanostructures, and metal ions. Quartz crystal microbalance analysis reveals that strong drug–carrier interactions contribute to the enhanced loading efficiency. In vitro and in vivo studies confirm the excellent biocompatibility and hemocompatibility of BP@MPN. Moreover, in a B16F10 tumor model, DOX-loaded BP@MPN combined with near-infrared (NIR) irradiation achieves potent chemo–photothermal synergistic therapy (TGI = 88.5%) with minimal systemic toxicity. These findings establish BP@MPN as a promising, broadly applicable nanocarrier platform for controlled drug delivery and advanced nanomedicine.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CEREAL SCIENCE
·
2026
·
IF 4.3
Kebing Yan,Xianqing Ye,Xiaoai Zhu,Yuan Ru,Yage Liu,Yiying Niu,Jun Xi,Kunlun Liu
Growing attention focuses on the glycemic index (GI) of staple foods, driven by the critical importance of blood glucose management for diabetic patients. This study investigated the influence of Flos Trollii polyphenol (FTP) on wheat noodle quality and starch digestion kinetics, focusing on its potential as a natural additive for improving nutritional value and reducing glycemic response. The incorporation of 0%–2% FTP enhanced noodle texture, reducing cooking loss by 33.5% and improving sensory attributes at concentrations ≤ 1.5%. Structural analyses (SEM, XRD, FTIR) revealed formation of V-type crystalline complexes between FTP and starch alongside gluten network reinforcement, thereby restricting digestive enzyme accessibility. DSC confirmed elevated thermal stability with gelatinization enthalpy increasing from 3.01 to 5.00 J/g, supporting starch-polyphenol interactions. In vitro digestion demonstrated that 1.5% FTP significantly decreased digestible starch content by 57% and predicted glycemic index (pGI) by 30.9% (65.18±1.09 compared to 94.40±3.15 in the control), primarily due to enzyme inhibition and complex formation. These findings highlight the dual role of FTP in enhancing noodle quality while reducing starch digestibility, providing a natural strategy for developing diabetes-friendly foods.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）