JOURNAL OF APPLIED ELECTROCHEMISTRY
·
2020
·Vol. 51
·期 2
·295-306
·
IF 2.384
Liu Qian,Zhang Binyan,Du Shilong,Li Lin,Liu Fuxin,Fan Weizhou,Liu Xiuhui
Abstract Excessive uptake of nitrite will damage the human health. Hence, there is a rising requirement to construction a highly sensitive electrochemical sensor for detecting the level of nitrite in food precisely. In this work, an electrochemical nitrite sensor was successfully constructed with the porous hollow carbon nanoparticles as the electrode material. The morphology and structure of the synthesized material were characterized by scanning electron microscopy, transmission electron microscope, Brunauer–Emmett–Teller, and Fourier transform infrared spectroscopy. Meanwhile, the electrochemical behavior of nitrite was studied by cyclic voltammetry and amperometry ( i − t ). As a result, the as-constructed sensor exhibited high electrocatalytic activity, high sensitivity (275.7 µA mM −1 cm −2 ), wider linear range (0.037–6950 μM), and lower limit of detection (10.41 nM) toward nitrite oxidation. Finally, the sensor was successfully applied to the detection of nitrite in pickle directly, indicating its feasibility for practical applications. The results showed that the porous hollow carbon nanospheres hold great prospect in sensitive detection of nitrite. Graphic abstract
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 413
·期 2
·469-478
·
IF 3.637
Yu Xiao,Yu Wanxiang,Han Xiufen,Chen Zuanguang,Wang Shumei,Zhai Haiyun
Doxorubicin has been extensively used to treat cancers, and there are recent findings that the anticancer activities can be enhanced by curcumin. Although the two compounds have native fluorescence, they can hardly be quantified directly simultaneously using the laser-induced fluorescence (LIF) detection method. To avoid complex fluorescence derivatization and introduction of interfering components, a highly sensitive double wavelength excitation source LIF (D-W-Ex-LIF) detector composed of a 445-nm and 488-nm commercial laser diode was constructed to detect them simultaneously. Rhodamine 6G was selected as an internal standard, because its fluorescence can be excited at 445 nm and 488 nm. The native fluorescence of doxorubicin and curcumin and their resolution were enhanced by introducing mixed micelles. The optimal electrophoretic separation buffer was 10 mM borate buffer containing 20 mM Triton X-100, 5 mM sodium dodecyl sulfate, and 30% (v/v) methanol at pH 9.00. Therefore, the developed method was specific, accurate, and easily operable. Its limits of detection for doxorubicin and curcumin in human urine samples were 4.00 × 10 −3 and 1.00 × 10 −2 μg/mL, respectively, and the limits of quantification were 1.00 × 10 −2 and 3.00 × 10 −2 μg/mL, respectively. The recoveries were 94.9–109.1%.
Graphical abstract
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高影响因子
SN Applied Sciences
·
2020
·Vol. 2
·期 11
·1-14
·
IF 0
Fan Zhinan,Zhang Chi,Ma Rongliang,Fan Li-Juan
Abstract Visualization of latent blood fingerprints (LBFPs) at violent crime scenes is very important for identifying criminals, while current reagents/methods for developing LBFPs still have more or less limitations. Here, we advance a new strategy for LBFP development based on the dye-soaked cotton pads as “ensemble” materials. The cotton pad was first soaked in the dye solution and then placed on the substrate with deposited blood fingerprint for development. After peeling off the pad, uniform and legible fingerprint patterns were obtained in all cases where different dyes and substrates were employed. The dye-soaked pads displayed superior developing effect compared with those using conventional methods (immersing, smearing and spraying). The pad-based method exhibited many other merits, such as little damage to the fingerprint patterns and less dye stains left on fingerprint and substrate, as well as the reusability. This pad-based method was also applicable for developing sebaceous latent fingerprints by replacing the small molecular dyes with fluorescent conjugated polymer nanoparticles. In all, the dye-soaked cotton pad developing protocol is feasible, cost-effective and universally applicable for developing different types of latent fingerprints on various substrates. Graphic abstract
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2021
·Vol. 105
·期 5
·2033-2042
·
IF 4.813
Wang Xiao,Xu Na,Li Qinglin,Chen Shengqi,Cheng Hui,Yang Mo,Jiang Ting,Chu Jun,Ma Xiaojing,Yin Dengke
Liver cancer, one of the most common types of cancer in the world, is the second leading cause of death for cancer patients. For liver cancer, there is an urgent need for an effective treatment with no or less toxic side effects. Lactonic sophorolipids (LSL), as a potential anticancer drug, has attracted wide attention of pharmaceutical researchers with its good biological activities. The effects of LSL and cell death inhibitors were measured by MTT test on HepG2 cells. Meanwhile, the morphology of the cells was observed under a microscope. The apoptosis rate was detected by flow cytometry, and the expression levels of enzyme activity of Caspase-3 and Caspase-9 were measured by detection kits. Meanwhile, mRNA levels of Apaf-1 , Caspase-3 , Bax , and Bcl-2 were measured by quantitative real-time RT-PCR; protein levels of Caspase-3, Cleaved Caspase-3, Bax, and Bcl-2 were measured by western blot. LSL can inhibit the proliferation of cells, and it is possible to induce apoptosis in cells. The HepG2 cells with LSL co-culture exhibited typical apoptotic morphology, and the expression levels of enzyme activity of Caspase-3 and Caspase-9 increased ( P < 0.05). We also found that LSL increases cell apoptosis rate and regulates the expression of genes and proteins associated with apoptosis through the Caspase-3 pathway. These results indicate that LSL may be one of the potential drug candidates to inhibit the proliferation and induce apoptosis in HepG2 cells.
Key points
• LSL, which is of good biological activities such as anti-bacterium, virus elimination, and inflammatory response elimination, has been firstly used to intervene in vitro to investigate its effect on HepG2 cell proliferation. • LSL can inhibit the proliferation of cells, and it is possible to induce apoptosis in HepG2 cells through the Caspase-3 pathway. • The mechanism of LSL action on HepG2 cell proliferation was firstly also discussed, which provides a certain experimental reference for the clinical treatment of liver cancer. Graphical abstract
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JOURNAL OF FLUORESCENCE
·
2021
·Vol. 31
·期 2
·339-348
·
IF 2.217
Lv Rongyao,Li Genrong,Lu Shuting,Wang Ting
Carbon dots are nano-sized photoluminescence materials which have good biocompatibility and low cytotoxicity, while the previously synthesized carbon dots lack tumor targeting capability and therapy function so that it cannot achieve the purpose of diagnosis and treatment. Herein, a new kind of multi-functional carbon dots (GFCDs) is promising to be applied in tumor cells imaging and clinical targeted therapy. Gallic acid (GA) was used as the carbon resource and antitumor active molecule, folic acid (FA) was used as the nitrogen resource and tumor targeting molecule, and citric acid monohydrate (CA) was used as the auxiliary carbon source. Multi-functional GACDs were synthesized by a simple one-step microwave-assisted procedure and analyzed with UV − vis spectrophotometer, fourier transform infrared spectrometer, transmission electron microscopy and X-ray photoelectron spectrometer. Results show that the diameter of GFCDs is about 3 nm. And GFCDs are pale-yellow under natural light which turn blue under 360 nm UV lamp. Besides ester bond is the connecting mode between functional molecules. In addition, the results of in vitro cell imaging experiments and in vivo antitumor experiments demonstrate the targeting imaging and antitumor abilities towards Hela cells. Graphical abstract
The synthesis route and properties of GFCDs.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2021
·Vol. 32
·期 2
·2061-2074
·
IF 2.478
Wang Xule,Song Jimei,Lu Yun,Zhu Wanrong,Hu Gang
In this work, a series of Z-scheme Ag/Ag 2 WO 4 /g-C 3 N 4 (AAC) photocatalysts were successfully constructed by the deposition–precipitation method at room temperature. The phase, structure, morphology and optical property of the as-prepared photocatalysts were well characterized. Interestingly, all AAC exhibited prominent photocatalytic activity for Rhodamine B (RhB) degradation under visible light illumination. Especially, AAC-3 possessed the higher RhB degradation rate ( k = 0.1855 min −1 ). More remarkably, when H 2 O 2 (1 mL 0.0977 mol/L) was dissolved into reaction system, the degradation rate of RhB was further improved ( k = 0.7899 min −1 ), which could be mainly attributed to the high separation rate of photogenic carriers and more active species. Meanwhile, a feasible photocatalytic reaction mechanism was proposed and discussed in detail.
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高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 28
·期 13
·16470-16479
·
IF 4.223
Wang Lifang,Chen Jingfan,Li Xian,Chen Letian,Zhang Kaige,Wang Xuefeng,Zhu Guifen
A novel eco-friendly molecularly imprinted polymer (MIP) was proposed as solid-phase extraction (SPE) adsorbent to selective adsorption tylosin (TYL) in animal muscle samples. The MIP was synthesized in aqueous by using 1,4-butanediyl-3,3-bis-1-vinyl imidazolium chloride and 2-acrylamide-2-methylpropanesulfonic acid as bifunctional monomer. The obtained MIP had excellent selectivity towards TYL in water, and the maximum binding capacity can reach 123.45 mg g −1 . Combined with high-performance liquid chromatography, the presented MIP can be used as SPE sorbent to recognize and detect TYL in the range of 0.008 to 0.6 mg L −1 ( R 2 = 0.9995). The limit of detection and limit of quantification were 0.003 mg L −1 and 0.008 mg L −1 , and the intraday and interday precision were 1.05% and 3.36%, respectively. Under the optimal condition, the established MIP-SPE-HPLC method was successfully applied to separate and determine trace TYL in chicken, pork, and beef samples with satisfactory recoveries ranged from 94.0 to 106.3%, and the MIP-SPE cartridge can be cycled at least 20 times. This study implies a promising green MIP-SPE-HPLC method for highly selective adsorption and analysis trace TYL in complex matrices.
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Horticulture Research
·
2021
·
IF 6.793
Zhou Wei,Shi Min,Deng Changping,Lu Sunjie,Huang Fenfen,Wang Yao,Kai Guoyin
Water-soluble phenolic acids are major bioactive compounds in the medicinal plant species Salvia miltiorrhiza. Phenolic acid biosynthesis is induced by methyl jasmonate (MeJA) in this important Chinese herb. Here, we investigated the mechanism underlying this induction by analyzing a transcriptome library of S. miltiorrhiza in response to MeJA. Global transcriptome analysis identified the MeJA-responsive R2R3-MYB transcription factor-encoding gene SmMYB1. Overexpressing SmMYB1 significantly promoted phenolic acid accumulation and upregulated the expression of genes encoding key enzymes in the phenolic acid biosynthesis pathway, including cytochrome P450-dependent monooxygenase (CYP98A14). Dual-luciferase (dual-LUC) assays and/or an electrophoretic mobility shift assays (EMSAs) indicated that SmMYB1 activated the expression of CYP98A14, as well as the expression of genes encoding anthocyanin biosynthesis pathway enzymes, including chalcone isomerase (CHI) and anthocyanidin synthase (ANS). In addition, SmMYB1 was shown to interact with SmMYC2 to additively promote CYP98A14 expression compared to the action of SmMYB1 alone. Taken together, these results demonstrate that SmMYB1 is an activator that improves the accumulation of phenolic acids and anthocyanins in S. miltiorrhiza. These findings lay the foundation for in-depth studies of the molecular mechanism underlying MeJA-mediated phenolic acid biosynthesis and for the metabolic engineering of bioactive ingredients in S. miltiorrhiza.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2020
·Vol. 22
·期 12
·1-10
·
IF 2.132
Zhang Sainan,Feng Min,Liu Ying,Wang Daoai
Ta 2 O 5 -TiO 2 heterojunction photocatalysts with a structure of TiO 2 nanodots deposited on Ta 2 O 5 nanotube (NTs) were prepared by anodization and in situ growth methods following annealing treatment. The heterojunction photocatalytic material exhibited enhanced photodegradation behavior of methylene blue (MB) and photoelectrochemical water splitting performance under simulated solar light. The degradation rate of MB and photocurrent density of heterojunction is 3.99 and 8.33 times higher than that of pure Ta 2 O 5 NTs respectively. The synergetic effect of the phase heterojunction and highly ordered structure increased the specific surface area and improved the separation and transmission efficiency of photogenerated electrons and holes, thereby enhancing the photocatalytic activities. Hence, Ta 2 O 5 TNs-TiO 2 nanodots heterojunction has promising application in the fields of photodegradation, photoelectrochemical water splitting, solar cells, and other photocatalytic devices. Graphical abstract
The heterojunction photocatalytic material exhibits enhanced photodegradation behavior of methylene blue (MB) and photoelectrochemical water splitting performance under simulated solar light. The degradation rate of MB is 3.17and 3.99 times higher than that of pure TiO 2 NTs and Ta 2 O 5 NTs, respectively; the photocurrent density increases by 8.33 times and 1.6 times compared with pure Ta 2 O 5 NTs and TiO 2 , respectively.
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JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 56
·期 3
·2763-2776
·
IF 3.553
Yu Yucong,Shi Xiaolong,Liu Lin,Yao Juming
Nowadays high porosity aerogels are widely applied in wastewater purification, while the fragility and unversatility of nascent cellulose aerogels limited the application in oil/water filtering separation. Here, highly compressible, elastic, superhydrophobic cellulose/poly(vinyl alcohol) (PVA) composite aerogels were fabricated via combination of chemical cross-linking, freeze drying and silanization. The obtained cellulose/PVA aerogels exhibited durable superhydrophobicity with WCA of 156.6 °, even maintaining 155.0 ° after 30 days. Synergistic effects of physical entanglement, chemical cross-linking and H-bond network between cellulose and PVA molecular chains endowed the aerogels with excellent compressibility and recoverability. The aerogels exhibited maximum compressive stress of 490.7 kPa at 90% strain, even without reduction in mechanical strength under 70% compressive strain for 10 cycles. More importantly, the strengthened aerogels exhibited remarkable separation performances for both immiscible oil/water mixtures and surfactant-stabilized water-in-oil emulsions. Fascinating permeation flux of up to 7176.3 L m −2 h −1 and total separation amount as high as 4550.6 L m −2 with separation efficiency of 98.5% for water-in-cyclohexane emulsion were harvested. Thus, this work provides a green, sustainable and mass-producible cellulose-based aerogel for high-efficiency oily wastewater remediation.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2020
·Vol. 22
·期 11
·1-12
·
IF 2.132
Wang Linjuan,Zan Ling
MoS 2 -modified In 2 S 3 ( x wt.%MoS 2 -In 2 S 3 ) samples (0 ≤ x ≤ 20) were fabricated through a facile one-pot hydrothermal process. All In 2 S 3 samples modified by MoS 2 deliver an excellent Cr 6+ removal ability under visible-NIR-light-driven, and 5% MoS 2 -In 2 S 3 can reduce 20 mg/L Cr 6+ solution completely within 30 min with a trace photocatalyst concentration (0.27 mg/mL) which is 3.2-hold higher than that of single In 2 S 3 . Further investigations demonstrate that the loaded MoS 2 can not only enhance the absorption of light scope but also promote the interfacial electron transfer from In 2 S 3 to MoS 2 and then to Cr 6+ , thus greatly improving the Cr 6+ removal ability. ESR shows that the modifying of MoS 2 can accelerate the transformation of electrons from the In 2 S 3 to MoS 2 which results in that the intensity of O 2 − • significantly increased. PL, photocurrent, and EIS further identified that the modification of MoS 2 can greatly improve the charge carrier separation efficiency. The fabricated MoS 2 -In 2 S 3 samples also exhibit characteristic features such as stable and reducing Cr 6+ in the weak alkaline environment for the first time, which could always be a great challenge for wastewater purification in alkaline condition.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2020
·Vol. 28
·期 7
·8486-8497
·
IF 3.056
Wei Xiuzhen,Zhang Qian,Cao Shiyu,Xu Xufeng,Chen Yi,Liu Lu,Yang Ruiyuan,Chen Jinyuan,Lv Bosheng
Nanofiltration (NF) membranes show great potential for advanced water treatment, especially for trace organic pollutant removal. The removal efficiency of pharmaceuticals and personal care products (PPCPs) and environmental estrogenic hormones (EEHs) by positively charged hollow fiber NF membranes (PEI-NF) were evaluated. The separation properties were evaluated by changing the operating pressure, temperature, ionic strength, and cation species. A relationship between the physicochemical characteristics of the pharmaceuticals and the NF membrane retention behavior was established. The results showed that the rejection rates of the PEI-NF membrane for the selected PPCPs and EEHs ranged from 81 to ~ 91.26%. For positively (negatively) charged pharmaceutical molecules, the electrostatic repulsion (attraction) effect and steric hindrance were the dominant rejection mechanisms of the PEI-NF membrane. For neutral pharmaceutical molecules, in addition to the size sieving effect, the hydration-induced size increase of hydrophilic substances improved the rejection rates. Both the molecular structure and diffusion coefficient of pharmaceutical molecules influenced their rejection by the PEI-NF membrane to a certain extent. Moreover, the PEI-NF membrane showed a high removal effect for PPCPs and EEHs in water samples from actual tap water plants.
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高影响因子
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