Journal of Analysis and Testing
·
2021
·Vol. 5
·期 1
·84-92
·
IF 0
Chen Shuting,Chen Chaoqun,Wang Jian,Luo Fang,Guo Longhua,Qiu Bin,Lin Zhenyu
In this work, a novel fluorescent biosensor has been constructed for rapid detection of Cu(II) via the interaction between the fluorophore groups on the surface of nitrogen-doped-carbon-dots (N-CDs) and ·OH produced from the catalytic reaction between Cu (II) and cysteine (Cys). Specifically, Cu (II) can catalyze the oxidation of Cys to form cystine (Cys–Cys) and hydrogen peroxide (H 2 O 2 ), and Cu (II) can also catalyze the decomposition of H 2 O 2 to produce hydroxyl radicals (·OH) by the Fenton-like reaction. ·OH can oxidize and destroy the surface structure of N-CDs, resulting in the fluorescence quenching of the N-CDs. Under the optimal experimental conditions, the linear range of Cu (II) is determined to be 0.05–25 μmol L −1 , and the limit of detection is 23 nmol L −1 with the limit of quantitation of 77 nmol L −1 . Besides, some characterizations are provided to verify the proposed principle. The method has been successfully applied for the detection of Cu (II) in human serum and environmental water with high sensitivity and higher selectivity.
✓ 已验证
产品关联
DOI
JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE
·
2021
·Vol. 30
·期 4
·2462-2472
·
IF 1.819
Zang Chao,Yang Ming,Jia Zhengfeng,Zhang Jing,Jiang Jinlong,Li Wei,Su Changhua,Zhang Ran
In this study, dopamine and graphene oxide (GO) solutions were spin-coated on the silicon wafers to obtain the GO/polydopamine (PDA) films. The carbon films were subsequently fabricated by annealing the GO/PDA films in argon atmosphere. The atomic force microscopy analysis was performed to characterize the morphology and tribological properties of the composite films. The developed carbon films exhibited lower friction coefficient and high adhesion between composite films and substrate as compared to the untreated films, which was attributed to the enhanced hydrophobicity, improved hardness and elastic modulus and chemical bonding of the films with substrate. The findings obtained in this study would lead to an efficient design of the anti-wear nanofilms for the micro-/nanoelectronic mechanical systems.
✓ 已验证
产品关联
DOI
高影响因子
SN Applied Sciences
·
2021
·Vol. 3
·期 3
·1-9
·
IF 0
Ma Yinzheng,Wen Yingying,Chen Jinping,Zhang Yunxia,Zhang Haiying,Sui Jinlei,Yi Guohui,He Xiaowen
A rapid and sensitive headspace gas chromatography-mass spectrometry (HS-GC–MS) method was established for the determination of benzyl isothiocyanate (BITC) in the peel, pulp, and seeds of Carica papaya Linn. Tween 80 solution with a concentration of 0.002% ( w/v ) was chosen as a headspace medium for solving the poor solubility of BITC in water without using organic solvents and ensuring high headspace efficiencies. Extraction parameters had been evaluated and optimized by using an orthogonal design with an OA 9 (3 4 ) table. Optimal headspace conditions were obtained when vials were equilibrated at 80 °C for 20 min at a stirring speed of 375 rpm. The calibration curve obtained by using GC–MS was linear in a concentration range of 10–320 ng/mL. The recoveries of peel, pulp, and seeds ranged from 97.3 to 100.6% with RSDs less than 3.0%. The method is simple, rapid, sensitive, and environmentally friendly. It is suitable for analyzing BITC in papaya fruit and is expected to have important application potential in the extraction of water-insoluble volatile components in foods, plants, medicines, and other samples.
✓ 已验证
产品关联
DOI
JOURNAL OF NANOBIOTECHNOLOGY
·
2021
·Vol. 19
·期 1
·1-14
·
IF 10.435
Yang Yuming,Han Yu,Sun Qiuyang,Cheng Jin,Yue Caixia,Liu Yanlei,Song Jie,Jin Weilin,Ding Xianting,de la Fuente Jesús M.,Ni Jian,Wang Xiaoqiang,Cui Daxiang
Background Gene and chemical therapy has become one of the rising stars in the field of molecular medicine during the last two decades. However, there are still numerous challenges in the development of efficient, targeted, and safe delivery systems that can avoid siRNA degradation and reduce the toxicity and adverse effects of chemotherapy medicine.Results In this paper, a highly efficient AS1411 aptamer modified, dsDNA and MMP-2 cleavable peptide-fabricated gold nanocage vehicle, which could load doxorubicin hydrochloride (DOX) and siRNAs to achieve a combination of tumor responsive genetic therapy, chemotherapy, and photothermal treatment is presented. Our results show that this combined treatment achieved targeted gene silencing and tumor inhibition. After nearly one month of treatment with DOX-loaded Au-siRNA-PAA-AS1411 nanoparticles with one dose every three days in mice, a synergistic effect promoting the eradication of long-lived tumors was observed along with an increased survival rate of mice. The combined genetic, chemotherapeutic, and photothermal treatment group exhibited more than 90% tumor inhibition ratio (tumor signal) and a ~ 67% survival rate compared with a 30% tumor inhibition ratio and a 0% survival rate in the passive genetic treatment group.Conclusion sThe development of nanocarriers with double-stranded DNA and MMP-2 cleavable peptides provides a new strategy for the combined delivery of gene and chemotherapy medicine. Au-siRNA-PAA-AS1411 exerts high anticancer activities on lung cancer, indicating immense potentials for clinical application.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2021
·Vol. 56
·期 16
·9678-9691
·
IF 4.22
Ma Zhongying,Zhang Zhihao,Wang Yuhua
Flame retardant is essential for cotton fabric because of the restrictions in applications caused by poor flammability. Herein, a novel flame-retardant coating assembled on the surface of cotton fabric consists of polyethyleneimine/g-C 3 N 4 and polyethyleneimine/phytic acid as the underlying insulating coating and the upper intumescent coating, respectively. Different amounts of the composite coatings were deposited onto the fabric by the layer-by-layer self-assembled technique. The char residue of TGA over 20 wt% has indicated the significantly enhanced performance of the thermal stability of all the coated samples and the achievement of the samples with composite coatings in a self-extinguished level proving through vertical combustion test. The samples prepared with binary hybrid coatings exhibited a high limiting oxygen index value of 30.5%. From the MCC test, the peak heat release rate and total heat release of (P/G + P/PA) 4+4 showed a significant decrease of 76.9% and 77.1%, respectively. All these results demonstrated that the composite coatings endowed the cotton fabric with outstanding thermal stability and flame retardancy.
✓ 已验证
产品关联
DOI
高影响因子
RARE METALS
·
2021
·Vol. 41
·期 1
·37-44
·
IF 4.003
Shi Ze-Di,Zhang Le,Ma Yue-Long,Zhou Tian-Yuan,Sun Bing-Heng,Shao Cen,Huang Guo-Can,Yin Shi-Tong,Hou Su-Dan,Yang Zeng-Wang,Chen Hao
银作为一种功能性材料, 由于其优良的导电性而用作于汽车电子的电接触材料。但是, 这种材料极易遭受大气腐蚀问题, 并导致结构的损坏和器件的失效。本文研究了应用于印刷电路板中导电银的腐蚀行为, 并从动力学和机理方面发现了影响材料结构损害最主要的环境因素(SO 2 污染物、温度和相对湿度(RH, Relative humidity))。相较于其他因素, 温度(55 °C)是运行环境中引起导电材料结构变化的最主要因素, 并且表面的腐蚀层厚度达到了最大值, 即 d film =15.56× 10 -5 μm。然而, 在腐蚀过程中, SO 2 污染物和RH促进了腐蚀物质的扩散, 并也会增加腐蚀产物层(Ag 2 S和Ag 2 SO 4 )的厚度。因此, 本文不仅大大增强了人们对于不同环境因素下导电材料结构损害的认识, 同时也为实际生产中的防腐设计和材料选择提供了重要的指导。 Graphical abstract
✓ 已验证
DOI
高影响因子
Nano Research
·
2021
·Vol. 15
·期 2
·1455-1465
·
IF 8.897
Wen Lijuan,Peng Yu,Wang Kai,Huang Zhihua,He Shiying,Xiong Ruiwen,Wu Longping,Zhang Fengtian,Hu Fuqiang
Glioblastoma (GBM) is one of the malignant brain tumors with high mortality and no curative treatments. Abnormally elevated vascular endothelial growth factor (VEGF) in GBM seriously disrupts the blood brain barrier (BBB) with an increased permeability, resulting in poor outcome and prognosis. RNAi interference has shown strong potential to inhibit VEGF expression, thus it is necessary to development an effective and safe gene delivery system possessing the ability to cross the BBB and target GBM cells. This study aims to explore the anti-GBM effect of angiopep-2 (Ap) peptide modified reactive oxygen species (ROS) cleavable thioketal (TK) linked glycolipid-like nanocarrier (CSTKSA) delivering anti-VEGF siRNA (R), termed as Ap-CSTKSA/R complexes. Ap functionalized modification produced an enhanced cellular uptake and a stronger bio-distribution of Ap-CSTKSA/R complexes in U87 MG cells and brain tumor tissues, respectively. Ap-CSTKSA/R complexes exhibited great superiority in GBM growth inhibition and finally translated into the longest survival period mainly via receptor-mediated targeting delivery, VEGF gene silencing accompanied with remarkable angiogenesis inhibition, and suppressed expression of caveolin-1 which is involved in BBB functional regulation in the occurrence and treatment of GBM. The study indicated that Ap functionalization on ROS-responsive glycolipid-like copolymer exhibits a promising and effective gene delivery platform for GBM targeted treatment.
✓ 已验证
产品关联
DOI
高影响因子
CHROMATOGRAPHIA
·
2021
·Vol. 84
·期 9
·845-854
·
IF 2.044
Guo Siyu,Wu Shanshan,Zhao Shanwen,Wang Xiaoxiao,Cai Tianpei,li Jianmin,Gong Bolin
Unique restricted access media-magnetic molecularly imprinted polymers (RAM–MMIPs) were prepared as magnetic solid-phase extraction (MSPE) material for florfenicol (FF) and are combined with HPLC–UV to extract FF from fetal bovine serum samples. Under the optimization conditions, the resulting materials had a uniform imprinted layer, acceptable adsorption amounts (22.92 mg/g), rapid binding kinetics (80 min) and satisfactory selectivity. In addition, RAM–MMIPs presented satisfactory recoveries (95.6–102.1%), relative standard deviation (RSD) (2.7–3.9%) and lower limits of detection (LOD) (10.5 μg/L). The method was applied to the real samples. Compared with other reported methods, this method exhibits the advantages of simple, fast and, efficient operation, using a relatively small amount of the RAM–MMIPs adsorbent. The developed method can enrich FF from fetal bovine serum samples. Graphic abstract
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 23
·5695-5702
·
IF 4.157
Yu Haili,Zhang Guihua,Cai Yanhua,Dong Faqin
The Berthelot reaction is a classic method for detection of ammonium (NH 4 + ) and atmospheric ammonia (NH 3 ) by using salicylic acid (SA) as the chromogenic substrate. However, there lacks a method for improving the activity of the Berthelot reaction to enhance the analytical performance for detection of NH 4 + and NH 3 . Here, five SA analogues with electron-withdrawing groups (-F) and electron-donating groups (-CH 3 and -OCH 3 ) at different positions of the aromatic ring have been chosen as the alternative to SA for Berthelot reaction. Among these analogues, 4-methoxysalicylic acid (4-OCH 3 -SA) shows the best colorimetric response and color change at a NH 4 + concentration of 30 μM, and the sensitivity of 4-OCH 3 -SA-based colorimetric assay for NH 4 + increases 1.75-fold compared with that of SA-based colorimetric method. This enhancement effect is attributed to the strong electron-donating property of 4-OCH 3 group, activating the two-step electrophilic aromatic substitution reaction in the Berthelot reaction. Additionally, visual and sensitive detection of NH 3 is realized, along with a low limit of detection down to 0.037 ppm. Furthermore, we demonstrate that this assay is reliable and practical for detection of NH 4 + and NH 3 in real water and air samples with good accuracy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 414
·期 18
·5255-5262
·
IF 4.157
Qian Hai-Long,Liu Fang,Liu Xue,Yang Cheng,Yan Xiu-Ping
The separation of amino acid (AA) enantiomers shows significance for chemistry, food, and biology, but remains challenging due to their similar properties. A promising nanoporous chiral covalent organic framework (COF) as a stationary phase for high-performance liquid chromatography (HPLC) suffers from the irregularity and widely distributed particle size of the chiral COF. Herein, we show the facile preparation of a chiral COF-monolith as a stationary phase for HPLC enantiomeric separation of AAs via orthogonal experiments. The CTzDa-monolith is prepared by the incorporation of the model chiral COF named CTzDa into the porous poly(ethylene dimethacrylate-co-methacrylate) monolith and reveals great permeability and mechanical stability. The corresponding CTzDa-monolithic column gives better chiral HPLC separation of AAs than the commercial Poroshell 120 chiral-T column. Thermal dynamic analysis and molecular docking calculations imply the involvement of stereoscopic hydrogen, π-π, and van der Waals interactions between the CTzDa and AAs during HPLC enantioseparation. The facile incorporation of the chiral COF into the porous monolith will promote the potential of a chiral COF as a stationary phase for HPLC. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Composites and Hybrid Materials
·
2021
·Vol. 4
·期 4
·1048-1058
·
IF 5.693
Chen Fang,Xiao Hua,Peng Zhong Quan,Zhang Ze Ping,Rong Min Zhi,Zhang Ming Qiu
To tackle the challenges of insufficient heat conduction capacity and thermally/mechanically induced internal damages of glass fiber reinforced epoxy composite (that traditionally serves as circuit board substrate), hexagonal boron nitride (h-BN) was functionalized using natural flavonoid dihydromyricetin (DMY) and then compounded with reversible Diels–Alder (DA) bonds crosslinked epoxy and glass fiber cloth (GFC) reinforcement to fabricate self-healing thermally conductive glass fiber cloth reinforced epoxy (GFREP) composite by a facile two-step coating method. Particularly, the GFC was coated with the solution containing high concentration modified h-BN, and then impregnated with dip coating solution containing lower concentration h-BN. The gradient variation of modified h-BN concentration not only effectively reduced the interfacial heat resistance, but also avoided excessive deterioration of mechanical strength. The produced GFREP composite exhibited excellent in-plane (6.22 W m −1 K −1 ) and through-plane (1.53 W m −1 K −1 ) thermal conductivities at 35.0 wt% h-BN content. The reversible DA reaction helped to realize interlaminar crack healing of the composite as characterized by high degrees of recovery of mechanical strength (70 ~ 85%) and thermal conductivities (62 ~ 89%). Besides, the attenuated thermal conductivity caused by interfacial debonding between copper clad and the composite in the model copper clad laminates can also be restored via the same mechanism. Lastly, the composite laminates proved to be coupled with controlled degradability in good solvent so that they can be recycled. This study offered an efficient way to prolong the service life of thermally conductive GFREP composite for integrated circuit packaging applications. Graphical abstract The reversible DA reaction at the crack interface proves to be able to recover the structural integrity, which pushes the physical reconnection of the separated h-BN micron sheets residing on the fractured surfaces at the same time and re-establishes the continuous phonon transfer pathways.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 21
·5309-5320
·
IF 4.157
Zhou Kunfeng,Han Hong,Sha Jingquan,Luan Shaozheng,Diao Yan,Dong Chunyao,Yang Jinghua
Although many satisfactory studies have been developed for biomolecule detection, the complexity of biofluids still poses a major challenge to improve the performance of nanomaterials as electrochemical sensors. Herein, unprecedented polyoxometalate-based metal-organic frameworks (POMOFs) with 8-fold meso-helical feature, [Ag 5 (trz) 4 ] 2 [PMo 12 O 40 ] (PAZ), were synthesized and explored as electrochemical sensors to detect dopamine (DA). To improve the conductivity of PAZ and the binding ability with single-walled carbon nanotubes (SWCNTs), the nanocomposite of carboxyl functionalized SWCNTs (SWCNTs-COOH) with nano-PAZ (NPAZ), NPAZ@SWCNTs-COOH, was fabricated, and transmission electron microscopy (TEM) shows that NPAZ can interact stably and uniformly with SWCNTs-COOH, owing to more defect sites on the surface of SWCNTs-COOH. The electrochemical result of NPAZ@SWCNTs-COOH/GCE towards detecting DA shows that the linear range was from 0.05 to 100 μM with a detection limit (LOD) of 8.6 nM (S/N = 3). Graphical abstract
A new electrochemical biosensing platform by combining 8-fold helical POMOFs with SWCNTs-COOH was developed for enhancing detection of dopamine for the first time, exhibiting the lowest detection limit to date.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）