SEPARATION AND PURIFICATION TECHNOLOGY
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2026
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IF 9.1
Yan Zhang,Yaoli Wei,Cristiano Varrone,Seongbong Heo,Jiating Hao,Zhihong Liu,Aijuan Zhou,Senlin Shao,Xiuping Yue
Coupling membrane extraction with chain elongation enables in-situ recovery of medium-chain fatty acids (MCFAs) from waste activated sludge, but interfacial instability and phase breakthrough remain major obstacles to practical application. To address this challenge, a thin-film composite (TFC) membrane was fabricated on a hydrophobic polytetrafluoroethylene (PTFE) porous membrane substrate via a polydopamine/polyethyleneimine (PDA/PEI) co-deposition strategy. TFC membrane significantly increased breakthrough pressure from 13 kPa (pristine PTFE) to 164–288 kPa, effectively preventing phase breakthrough. Notably, during the reactive extraction of MCFAs, the dense films in the TFC membrane did not hinder mass transfer; instead, enhanced both extraction efficiency (from 71.0% to 95.9%) and selectivity. These improvements arise from the enlarged interfacial reaction area provided by the rough film surface and the stronger binding of short-chain fatty acids to hydrophilic functional groups of the film, as confirmed by density functional theory (DFT) calculations. When integrated into a real chain elongation system, the adoption of the TFC membrane increased caproate production by 50%, accompanied by clear environmental and economic benefits (indicating promising economic prospects, with a net profit of 0.795 USD m −3 ). To our knowledge, this represents the first demonstration of TFC membranes in membrane extraction, revealing their unique ability to couple stability, efficiency, and selectivity, and opening new opportunities for sustainable MCFAs bioconversion.
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DALTON TRANSACTIONS
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2025
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IF 3.3
Yuan Xu,Ya-Tao Hu,Jiaping Cao,Dixin Lin,Qiqi Zhong,Yi Wang,Hongdong Shi,Qianling Zhang
To address the challenges associated with low cellular uptake efficiency of metal-based anticancer agents and the suboptimal therapeutic efficacy of photodynamic therapy (PDT), a series of polypyridyl ruthenium(II) complexes (Ru Cn) with tunable carbon chain lengths were rationally designed and synthesized through the incorporation of fatty acid moieties, which confer enhanced lipid solubility and membrane-targeting capabilities. Among these, the octadecanoic acid-modified ruthenium complex (Ru-C18) exhibited superior anticancer activity. Cytotoxicity assays demonstrated that Ru-C18 displayed sub-molar cytotoxic potency under light irradiation. Co-localization studies confirmed its preferential accumulation in lysosomes after cellular internalization. Upon light activation, Ru-C18 efficiently generated singlet oxygen and induced hallmark features of pyroptosis, including plasma membrane blebbing, cellular swelling, and Gasdermin-dependent membrane pore formation. Consequently, Ru-C18 significantly suppressed tumor cell proliferation via activation of the pyroptotic pathway. These findings offer new insights into the rational design of highly effective and selectively targeted photodynamic anticancer agents.
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PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
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IF 6.6
Wenchao Liu,Yize Yin,Lijuan Wang,Chung Lim Law,Linlin Li,Weiwei Cao,Xu Duan,Guangyue Ren
This study examined how fatty acids of varying chain lengths influence the microwave freeze-drying (MFD) properties and cross-linking interactions in wheat starch-fatty acid complexes. The study revealed that fatty acids with extended carbon chains decreased the initial water content in wheat starch-fatty acid blends, thereby reducing the drying time. Long-chain complexes maintained a more stable freeze-drying rate with changes in experimental power compared to short-chain ones. When microwave power was raised from 0.5 to 2.5 W/g, the enthalpy shifts (ΔH) in melting peaks for starch-fatty acid complexes varied significantly: butyric acid blends showed a change of 176.71 J/g, octanoic acid 714.06 J/g, and myristic acid 1102.02 J/g. XRD revealed reductions in relative crystallinity by 12.87 %, 19.38 %, and 27.00 %. Raman spectroscopy showed peak width increases at 480 cm −1 of 8.22, 8.49, and 10.43, respectively. The extent to which wheat starch-fatty acid complexes were affected during MFD increased with the chain length of the fatty acids.
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Food Bioscience
·
2025
·
IF 5.9
Tong Zhou,Qingqing Chai,Man Zhang,Heping Cui,Mingzhou Song,Khizar Hayat,Xiaoming Zhang,Chi-Tang Ho
Alanine (Ala)-xylose (Xyl) Amadori compound (A-X-ARP) was found to increase the yields of 1-octen-3-ol and enals derived from linoleic acid oxidation, during the thermal processing of Agrocybe aegerita enzymatic hydrolysates (AEH). To elucidate the key intermediate contributing to this enhancement, model systems combining linoleic acid with A-X-ARP, Ala, and glyoxal (GO) were established, and the reaction products were analyzed. Ala, regenerated through the decomposition A-X-ARP, was identified as a crucial factor driving the production of enals and 1-octen-3-ol. Further thermal reaction models of linoleic acid with amino acids featuring diverse side chains revealed the universal catalytic effect of amino acids on enals and 1-octen-3-ol formation. Moreover, the amino group within amino acids was determined to be the key functional group responsible for this catalytic activity. Notably, the presence of amino acid was observed to reduce the oxygen dependence of linoleic acid oxidation in producing 1-octen-3-ol and enals. As a result, the addition of A-X-ARP during the thermal treatment of AEH increased the yield of 1-octen-3-ol, a compound with a typical mushroom-like note, from 19.3 ± 3.7 (OAV = 19) to 71.4 ± 9.8 μg/L (OAV = 71). These findings provided new insights for the development of mushroom-flavored seasonings.
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Food Bioscience
·
2025
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IF 5.9
Yangjincan Liu,Yuqing Huang,Haiming Hu,Huabing Yang,Lei Xiong,Junping Zheng,Tianxiang Zhu,Zhigang Zhang,Hongtao Liu
s Chenpi is a traditional medicinal and edible food in China. Chenpi polysaccharide (CP) is one of the main active components of Chenpi. This study established a fermentation process using Bacillus licheniformis to obtain fermented Chenpi polysaccharides (FCP) with a reduced degree of polymerization. FCP is an acidic polysaccharide exhibiting a molecular weight of 3720 Da. The three predominant monosaccharides in FCP were arabinose (31.75%), galactose (24.77%), and galacturonic acid (20.94%). According to comprehensive NMR studies, its main chain primarily comprised two types of residues: →4)-α-GalA p- (1→ and →4)-α-Gal p , while its side chains started with →2)-α-Fuc p- (1→ or →2)-β-Rha p- (1→ residues, which were further linked to extending residues such as →4)-β-Ara p- (1→ or →5)-α-Ara f- (1→, and ultimately terminated with terminal residues T-β-GalA p , T-β-Glc p , or T-α-Ara f . Compared to commercial pectic polysaccharides (PP), FCP exhibited higher solubility, a looser structure, and lower viscosity. In vitro gut microbiota fermentation demonstrated that FCP substantially enhanced the proliferation of beneficial bacteria, including Paraprevotella and Lachnospiraceae , while suppressing pathogenic bacteria like Shigella . Furthermore, FCP enhanced short-chain fatty acids (SCFAs) production by gut microbiota, particularly acetic and propionic acids. In the single-strain fermentation experiment, FCP promoted the growth of Lactococcus lactis , Pediococcus acidilactici , Parabacteroides distasonis , and Bacteroides thetaiotaomicron . Metabolomic analysis of Bacteroides thetaiotaomicron fermentation broth further revealed that FCP altered the composition of bacterial metabolites, enhancing the biosynthesis of beneficial metabolites, including propionate, 4'-O-methylglucoliquiritigenin, and cyclokievitone hydrate. This study indicated that B. licheniformis fermentation improves the physical and prebiotic properties of CP, providing new insights for the bioprocessing of Chenpi.
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FOOD HYDROCOLLOIDS
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2025
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IF 12.4
Jilong Lu,Meizhen Xie,Lipin Chen,Yong-Huan Yun
Colorimetric indicators of real-time food spoilage detection are typically fabricated using chromogenic agents or nanoparticles; however, their potential migration into food raises considerable safety concerns. Although structurally colored functional biomaterials, which do not pose such risks, represent promising alternatives, their application in food spoilage detection remains largely unexplored. In this study, a structurally colored chitosan membrane (CSM) was obtained from shrimp shells, and its responsiveness to acids and aldehydes was investigated. Fresh coconut water was used to evaluate the performance of CSM as a colorimetric spoilage indicator. Results indicate that CSM is a mesoporous material with a Bouligand structure and a deacetylation degree of 83.93 %. The specific surface area, average pore volume, and average pore size of CSM were 0.44 m 2 /g, 0.001 cm 3 /g, and 9.67 nm, respectively. CSM exhibited chromatic responsiveness to acids at a low pH of 3 due to the protonation of –NH 2 groups, as well as to aldehydes via the formation of imine bond (C=N). Acid and aldehyde exposure altered the crystalline polymorphism of chitosan in CSM. After 18 h of storage at room temperature, the membrane successfully detected spoilage in fresh coconut water through visible color changes, which can be attributed to the synergistic effects of organic acids and aldehydes accumulated during spoilage. These findings demonstrate the effectiveness of CSMs in detecting food spoilage. To our knowledge, this study is the first to demonstrate the potential application of structurally colored CSMs for detecting food spoilage, offering a novel approach for developing food spoilage indicators.
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Frontiers in Pharmacology
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2025
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IF 4.8
Yeshi Yin,Changhui Zhao,Qin Xiang,Zongyan Li,Xiu Liu,Changhui Hu,Rong Yu
Background: Animal and cell studies have demonstrated that Zuogui-Jiangtang-Yishen decoction (ZGJTYS) has a favorable effect on the treatment of diabetic kidney disease (DKD). Our previous clinical research also showed that ZGJTYS prevents DKD in a manner similar to that of benazepril. Nevertheless, the interactions between ZGJTYS and the human gut microbiota require further investigation, particularly its interference in the intestinal flora response to food ingredients that may increase DKD risk, such as L-α-phosphatidylcholine and L-tyrosine.Objective: The aim of this study was to evaluate the regulatory function of ZGJTYS on human gut microbiota and explore the effect of ZGJTYS on the intestinal flora response to L-α-phosphatidylcholine and L-tyrosine.Methods: ZGJTYS was prescribed from the First Affiliated Hospital of Hunan University of Chinese Medicine. High-throughput sequencing of bacterial 16S RNA genes and fungal internal transcribed spacer (ITS) sequences was used for intestinal flora analysis. An in vitro gut microbiota simulation model was used to investigate the effect of ZGJTYS on the intestinal flora’s response to L-α-phosphatidylcholine and L-tyrosine. Ultra-high-performance liquid chromatography and mass spectrometry were used for non-targeted metabolomics analysis.Results: Compared to the control group, the microbial diversity of DKD was significantly reduced by ZGJTYS treatment; three bacterial genera, including Parabacterioids, were significantly higher; eight bacterial genera, including Prevotella_9, and the linoleic acid content were significantly lower. A receiver operating characteristic curve analysis using Parabacterioids and Prevotella_9 showed an area under the curve greater than 0.75, indicating good predictive performance. ZGJTYS intervention restored some of the normal bacterial genera, such as Rickettsia and Metarhizium, which were regulated by L-α-phosphatidylcholine and L-tyrosine. Furthermore, ZGJTYS effectively restored several significantly different Kyoto Encyclopedia of Genes and Genomes metabolic pathways related to immunity and disease to normal, such as efferocytosis and tryptophan metabolism.Conclusion: ZGJTYS was found to effectively restore the microbiota that were altered by L-α-phosphatidylcholine and L-tyrosine to normal, along with their metabolites. However, the mechanism by which ZGJTYS exerts its preventive and therapeutic effects on DKD through the gut microbiota still requires further study.
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Food & Function
·
2025
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IF 5.4
Le Wang,Houzhen Lu,Haitao Gui,Zifu Ni,Zhongke Sun,Zihua Wang,Zhihua Wang,Xuanyan Liu,Qipeng Yuan
Ulcerative colitis (UC) is a prevalent inflammatory bowel disease (IBD) posing a significant health threat. This study explored the protective effects of D-tagatose against DSS-induced colitis in mice and its underlying mechanisms using H&E staining, AB-PAS staining, immunofluorescence, immunohistochemistry, ELISA, qPCR, western blotting, and other assays. D-Tagatose improved colitis by increasing body weight and colon length, with decreased DAI (disease activity index) and histopathological scores. The results showed that D-tagatose inhibited the secretion of myeloperoxidase (MPO), inflammatory enzymes (iNOS and COX-2) and pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) as well as increased the content of anti-inflammatory cytokines (IL-10) in vitro. In addition, D-tagatose enhanced the expression of tight junction proteins (ZO-1 and Occludin) and mucin (MUC-2). Furthermore, D-tagatose was able to modulate the gut microbiota dysbiosis caused by DSS-induced UC and increased the content of short-chain fatty acids (SCFAs). This study indicated that D-tagatose attenuated DSS-induced UC by modulating inflammatory cytokines, restoring intestinal barrier function, maintaining gut microbiota homeostasis, and enhancing SCFA production. These findings provide D-tagatose as a safe and effective novel functional food strategy for the prevention and treatment of UC.
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FOOD HYDROCOLLOIDS
·
2025
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IF 12.4
Pan Wang,Bangfeng Yin,Ping Zou,Zhenjia Zheng,Dan Wang,Peiyou Qin,Wenting Zhao,Yuanyuan Zhao,Ye Liu,Xiaoyan Zhao
To address the limitation of resveratrol (Res) related to its low bio-accessibility and poor stability, Res-pectin complexes with varying degrees of esterification were developed. The encapsulation efficiency of Res in these complexes was determined, revealing the following order: Res-L106 > Res-L13 > Res-H121 > Res-H115. Upon non-covalent interaction with pectins, Res exhibited enhanced stability, reduced crystallinity, and modified microstructure. The complexes exhibited stable particle sizes across a pH range of 4 to 8, with the Res-L106 complex showing minimal sensitivity to variations in ionic strength and temperature. All complexes exhibited improved antioxidant activities compared to pectins alone, with the Res-L106 complex displaying the highest activity. The complex enhancing the bio-accessibility of Res follows the order of Res-L106 > Res-L13 > Res-H121 > Res-H115. Additionally, all complexes promoted the production of short-chain fatty acids (SCFAs), with butyric acid being the predominant product. Notably, the Res-L106 complex exhibited the highest SCFAs-producing capacity. In conclusion, this study provides valuable insights into the design and optimization of strategies to enhance the bioavailability of Res, highlighting the potential of low-esterified pectin as an effective delivery carrier for bioactive compounds.
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ACS Applied Materials & Interfaces
·
2025
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IF 8.2
Weijie Zhang,Lintao Wang,Wei Pei,Jie Dang,Yewen Zhang,Xiang Liu,Abdullah Maqsood,QinQin He,Min Li
Introducing defects into Metal–organic frameworks (MOFs) is an effective measure to increase adsorption sites, which is of great significance for improving phosphorus (P) adsorption performance. In this study, various defect-containing cerium (Ce)-based MOFs (Ce-MOFs) were prepared by introducing different types and equivalents of monocarboxylates. Among them, butyric acid (BA)-regulated Ce-MOF (Ce-MOF(40BA)) exhibited excellent adsorption performance with a maximum phosphate adsorption capacity of 117.28 mg P/g. When the initial phosphate concentration was 3 mg P/L, the adsorption equilibrium could be reached within 10 min with a phosphate removal rate of 96.6%. Thermogravimetry analysis (TGA) showed that the average coordination number of Ce-MOF (40BA) was only 4.33 compared to six of the pristine Ce-MOF, indicating an abundant defect content. By increasing defects, mesopores were introduced, enriching the material’s pore structure and achieving a mesopore proportion up to 58.81%. X-ray photoelectron spectroscopy (XPS) analysis revealed that the introduction of defects created numerous unsaturated metal centers, leading to a significant increase in Ce(III) contents. Further density functional theory (DFT) calculations indicated that the defective Ce-MOF had a lower binding energy compared to the pristine Ce-MOF, making it more favorable for phosphate adsorption. This study provides a strategy for preparing efficient defect-containing MOF adsorbents. The excellent adsorption performance of Ce-MOF(40BA) in practical wastewater bodies makes it a promising material for solving water eutrophication.
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PubMed
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2025
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IF 4.6
Yawen Zhang,Hongli Zhang,Jiangong Ma,Jing Zhao,Huajuan Yin,Xu Jing,Xiaowen Wang
With the advent of new-generation green extraction solvents, deep eutectic solvents (DESs) present significant prospects for the extraction of bioactive substances. In this study, saponins were extracted from oats using an ultrasonic-assisted method with green DESs, replacing conventional toxic organic solvents. Adding sodium chloride markedly reduced solubility, resulting in the separation of two phases from the extract: DES and water. The saponins were separated into the water phase, while the DES was reused in subsequent extractions, supporting extractant recycling and promoting sustainable development. Adding salt offers a more gentle, convenient, and cost-effective alternative to current phase separation methods, such as pH adjustment, temperature variation, and carbon dioxide or nitrogen gas application. Optimizing the extraction and recovery parameters yielded the highest oat saponin extraction efficiency at 18.73 mg g –1 , and the recovery was 86.4 %. When compared with ethanol extracts, DES extracts exhibited superior antioxidant activity. Lastly, the greenness of this extraction method was confirmed through three greenness evaluation programs of greenness. The extraction method based on emerging responsive solvents can be extended to other hydrophilic bioactive substances.
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JOURNAL OF CHROMATOGRAPHY A
·
2025
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IF 4
Huanhuan Jiang,Qibin Li,Yang Yang,Jingcong Dai,Li Wang,Richard D Cannon,Xiaojing Dong,Ting-li Han,Lina Hu
Short-chain fatty acids (SCFAs) are prominent regulators of host physiology and are closely related to the pathophysiology of many metabolic diseases. SCFAs are predominately generated by microbial fermentation of dietary fiber in the gut. Therefore, efficient, reproducible, and affordable analytical methods are needed to identify and quantify SCFAs in a complex fecal sample. In this study, five different SCFAs (acetic acid, butyric acid, propionic acid, valeric acid, and isobutyric acid) were compared using six different extraction methods (HCl-DE, NaOH-Hexane, H 3 PO 4 -Butanol, H 3 PO 4 -EA, NaHCO 3 -MTBE, and SPME) based on gas chromatography-mass spectrometry (GC-MS) analysis. The specificity, linearity, recovery, precision, and sensitivity of different analytical approaches were systematically assessed using SCFA chemical standards and further validated with human fecal samples, ensuring cross-species applicability. Our results revealed that no single method performed optimally for all SCFAs, but rather, distinct methods exhibited compound-specific advantages. Moreover, this study provides a practical guideline for balancing recovery, reproducibility, and extraction efficiency for method selection based on research purposes. The H 3 PO 4 -Butanol method displayed superior recovery accuracy for valeric acid (100.16 ± 0.81% and 101.82 ± 4.83% recovery rate for low and median spiked concentrations, respectively). It also exhibited minimum intra-day repeatability (0.92% to 5.67% RSD values) and good linearity (R 2 =0.94-0.99) across chemical mixtures. However, it showed the best extracted efficiency for butyric acid and best sensitivity for isobutyruc acid in fecal samples. Moreover, the H 3 PO 4 -EA method showed the highest recovery accuracy for isobutyric acid (103.21 ± 11.07% to 104.79 ± 3.59% recovery rate for all three different spiked concentrations) and the smallest inter-day repeatability (3.37% to 7.25% RSD values). The SPME method exhibited superior recovery accuracy for acetic acid (81.16 ± 21.17% to 94.08 ± 10.92% recovery rate for all three different spiked concentrations) and extracted the highest levels of SCFAs for acetic acid, propionic acid, and isobutyric acid. Finally, the application of these methods in human clinical samples revealed significant alterations of SCFA levels in patients with missed abortion, supporting their potential for translational studies. Overall, our results indicated that H 3 PO 4 -Butanol and SPME are the recommended methods for studying SCFAs in fecal samples.
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