JOURNAL OF BIOMATERIALS APPLICATIONS
·
2025
·
IF 2.5
Parvez Ahmad,Sangeeta Devi Khangembam,Anil Kumar Gangwar,Vipin Kumar Yadav,Prafull Kumar Singh,Yogendra Singh,Ravi Prakash Goyal,Surendra Pratap Chakraverty,Rajesh Kumar Verma
Biological scaffolds prepared after decellularization are used for the restoration of damaged tissues. A number of chemicals are used for bioscaffold preparation, and some of them damage their composition and architecture. Herein, we investigated the Sapindus mukorossi fruit pericarp extract (SPE) (5%) for decellularization of the caprine dermis. The dermal samples were processed in 5% SPE over magnetic stirrer for 96h at room temperature. The decellularization efficiency of SPE was analyzed by histological examination, DAPI staining, scanning electron microscopy (SEM), quantification of DNA hydroxyproline and hemocompatibility determination. Further, these acellular caprine dermal scaffolds were transplanted on full thickness skin wounds of group III New Zealand white rabbits. The wounds were left open in group I (Sham) and reconstructed by autograft in group II (n = 6 in each group). Continuous agitation of native caprine dermal tissues in 5% SPE for 96 hours leads to complete decellularization without affecting the extracellular matrix architecture. Microscopic observation of decellularized samples did not show any nuclei. DNA quantity was reduced (p < .05) in decellularized samples and scaffolds were found to be hemocompatible. Complete healing was observed on day 28 in groups II and III. No significant difference was noted in IgG in all the groups. Quantitative assessment of MDA showed a significant increase in groups I and II. Our results suggested that the 5% SPE solution effectively decellularized the native caprine dermis and the scaffolds were well tolerated by the animals.
研究领域:
组织工程
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再生医学
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生物材料学
✓ 已验证
DOI
PubMed
高影响因子
Biomedical Materials
·
2019
·Vol. 14
·期 6
·065007
·
IF 3.44
Ruifang Zhao,Zunkai Xu,Bing Li,Tao Chen,Naibin Mei,Chuang Wang,Zongbao Zhou,Lingling You,Chaoxi Wu,Xiaoying Wang,Shunqing Tang
The development of degradable polymer scaffolds is a key issue in bone regeneration. Poly(D, L-lactide) (PDLLA) and its derivatives have usually been applied to the construction of degradable scaffolds, but these scaffolds had problems with acidic degradation products and quick loss of mechanic strength during the later degradation, which usually led to scaffold collapse and cavity formation because of the slower rate of bone regeneration. In the present paper, a polysaccharide derivative, agarose acetate (AGA), was synthesized and a novel porous AGA scaffold was successfully developed through a salt-leaching process. The AGA scaffold had over 90% porosity without swelling in water, and compared to collapse and acidic products of PDLLA scaffold during degradation, the AGA scaffold maintained a stable morphology and a nearly neutral pH value over 18 months' degradation in PBS. A bone mesenchymal stem cells (BMSCs) adhesion and proliferation experiment showed that more cells adhered to the AGA scaffold than to the PDLLA scaffold. A subcutaneous implant test showed that the AGA scaffold slowly degraded and did not cause an inflammatory response surrounding the implantation lesion site. AGA scaffold was implanted into femur defects in New Zealand white rabbits to test its in vivo performance. Results indicated that the AGA scaffold accelerated the process of bone regeneration compared to the PDLLA group and, with time, new bone was formed from the margin toward the center of the scaffolds, and the scaffold left in place retained its porous structure without collapsing. Meanwhile, the AGA scaffold showed a low degradation rate and kept its shape during the in vivo degradation compared to the PDLLA scaffold. This performance could have the benefit of integrated regenerative bone being formed instead of cavities due to the quickly degraded scaffold disappearing. These results demonstrate that the AGA scaffold has significant potential in bone regeneration applications.
研究领域:
组织工程
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生物医用材料
·
骨科
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2022
·
IF 3.318
Jingjing Yang,Zhen Liang,Ping Lu,Fei Song,Zhen Zhang,Huiyun Xia,Jijun He,Tianyang Zhou,Junjie Zhang
Luliconazole (LCZ) is a novel antifungal imidazole with broad-spectrum and high susceptibility of Aspergillus and Fusarium are the dominant species of fungal keratitis, may potentially be a new medical treatment option for ocular fungal infection. To evaluate LCZ distribution in ocular tissues after topical application for the development of ophthalmic delivery system, it is important to have a bioanalytical method for measuring the drug concentrations in different ocular tissues and aqueous humor (AH). A selective and sensitive ultrahigh performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) method was developed for the quantification of LCZ in rabbit ocular tissues, including conjunctiva, cornea, AH, iris, lens, vitreous humor (VH), retinal choroid and sclera, using lanoconazole as internal standard (IS). Chromatographic separation was achieved on a Xterra MS, C18 column (2.1 × 50 mm, 3.5 μm) using mobile phase with formic acid solution (0.2%, v/v): acetonitrile (50:50, v/v) at a flow rate of 0.2 ml/min, and the run time was 2.5 min. Detection was performed using the transitions 354.1 → 150.3 m / z for LCZ and 320.1 → 150.3 m / z for IS by positive ion electrospray ionization in multiple reaction monitoring (MRM) mode. Method validation was conducted in accordance with U.S. Food and Drug Administration’s regulatory guidelines for bioanalytical method validation. The calibration curves were linear over the concentration range from 2.80 ng/ml to 2038 ng/ml for conjunctiva, cornea and sclera, 2.09 ng/ml to 1019 ng/ml for AH, 2.09 ng/ml to 509.5 ng/ml for iris, 2.09 ng/ml to 203.8 ng/ml for retinal choroid and VH, 2.04 ng/ml to 101.9 ng/ml for lens, with all the squared correlation coefficients (r 2 ) more than 0.99. The accuracy of the method was within the acceptable limit of 89.34%∼112.78% at the lower limit of quantification and other concentrations, Inter-day and intra-day precision values, expressed in terms of RSD (%), in all tissues were within 15% at all concentrations. The mean recoveries of LCZ in rabbit ocular tissues was 84.85%∼100.52%. No interference was found due to matrix components. Luliconazole was stable during the stability studies, including autosampler stability, benchtop stability, freeze/thaw stability and long-term stability. The method was successfully applied to the ocular pharmacokinetic and tissues distribution studies of LCZ in rabbit after topical administration of LCZ ophthalmic drug delivery system.
研究领域:
药理学
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分析化学
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眼科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Wu Zou,Fuchun Gong,Xuejiao Chen,Zhong Cao,Jiaoyun Xia,Tingting Gu,Zhizhang Li
Intrinsically fluorescent polymer nanoparticles (F-PNPs) were synthetized from 2-hydroxy-5-methylisophthalaldehyde and melamine by solvothermal method . F-PNPs can emit strong yellow green fluorescence at 542 nm without the conjugation to any external fluorescent agent and surface modification. Owing to the abundant amino and hydroxyl groups on their surface, the F-PNPs possess multiple binding sites, good biocompatibility and excellent water-solubility. Addition of Zn 2+ to the F-PNPs solution resulted in a blue shift (Δλ=40 nm) with obvious enhancement in the fluorescence intensity at 502 nm; while there was negligible change in the presence of other metal ions . The subsequent treatment with pyrophosphate (PPi) can cause fluorescence recovery of F-PNPs by pulling the Zn 2+ out of the coordination cavity of F-PNPs-Zn 2+ nanocomposites. No interference was observed from other anions and nucleotides, making the F-PNPs-Zn 2+ ensembles highly sensitive and selective nanoprobes for PPi. The detection limit is 2.75 × 10 −8 M/L and 7.63 × 10 −8 M/L for Zn 2+ and PPi, respectively. The proposed nanoprobes were then used for detecting the recovery of Zn 2+ and PPi in rabbit serum samples, which were found to be 99.4–104.2% and 98.6–104.7%, respectively. The present strategy for the fabrication of nanoparticles may offer a new sight for the preparation of polymer nanostructures . The F-FNPs based probes can provide an accurate method for the detection of Zn 2+ and PPi in serum samples.
研究领域:
生物化学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Zefu Wang,Zhifei He,Dong Zhang,Xiaosi Chen,Hongjun Li
This study aimed to assess the protective effects of the main components of Zanthoxylum bungeanum Maxim. essential oil (ZBMEO) on the characteristics of rabbit meat myofibrillar protein (MP) under MDA (malondialdehyde) induced oxidation. The MP suspensions were mixed with various concentrations (5 mM, 25 mM and 50 mM) of linalool, limonene, sabinene under MDA-induced oxidation. The retained MDA contents were determined, and the thermal stability and structure of rabbit meat myofibrillar protein were analyzed. Linalool, limonene and sabinene can prevent MDA from binding to MP effectively. The addition of linalool, limonene and sabinene led to the increase in the sulfhydryl and free amine contents, and the decrease in the carbonyl content and surface hydrophobicity compared to control MP. Also, the addition had protective effects on maintaining the secondary structure and morphology of MP under MDA-induced oxidation. The Differential scanning calorimetry (DSC) and SDS-PAGE analysis indicated that linalool, limonene, sabinene had an obvious protective effect on MHC and actin. The above results suggest that linalool, limonene and sabinene could reduce the oxidative damage of MDA to MP, which might have the potential in protecting the structure and physicochemical properties of the protein in foods .
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2022
·
IF 7.033
Qingxin Fan,Hao Wu,Qingquan Kong
Background The frequent occurrence of failed back surgery syndrome (FBSS) seriously affects the quality of life of postoperative lumbar patients. Epidural adhesion is the major factor in FBSS.Purpose A safe and effective antiadhesion material is urgently needed.Methods A superhydrophilic PLGA-g-PVP/PC nanofiber membrane (NFm) was prepared by electrospinning. FTIR was performed to identify its successful synthesis. Scanning electron microscopy, thermogravimetric analysis, differential scanning calorimetry, and water contact angle measurement were performed. CCK-8 assays were performed in primary rabbit fibroblasts (PRFs) and RAW264.7 cells to explore the cytotoxicity of PLGA-g-PVP/PC NFm. Calcein-AM/PI staining was used to measure the adhesion status in PRFs. ELISA was performed to measure the concentrations of TNF-α and IL-10 in RAW264.7 cells. In addition, the anti-epidural adhesion efficacy of the PLGA-g-PVP/PC NFm was determined in a rabbit model of lumbar laminectomy.Results The PLGA-g-PVP/PC NFm exhibited ultrastrong hydrophilicity and an appropriate degradation rate. Based on the results of the CCK-8 assays, PLGA-g-PVP/PC NFm had no cytotoxicity to PRFs and RAW264.7 cells. Calcein-AM/PI staining showed that PLGA-g-PVP/PC NFm could inhibit PRF adhesion. ELISAs showed that PLGA-g-PVP/PC NFm could attenuate lipopolysaccharide-induced macrophage activation. In vivo experiments further confirmed the favorable anti-epidural adhesion effect of PLGA-g-PVP/PC NFm and the lack of a strong inflammatory response.Conclusion In this study, PLGA-g-PVP/PC NFm was developed successfully to provide a safe and effective physical barrier for preventing epidural adhesion. PLGA-g-PVP/PC NFm provides a promising strategy for preventing postoperative adhesion and has potential for clinical translation.
研究领域:
生物医学工程
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材料科学
·
神经外科
✓ 已验证
DOI
PubMed
高影响因子
Heliyon
·
2024
·
IF 3.4
Yingjie Wu,Mingxiang Fan,Shixiang Tan,Qiucheng Guo,Hegui Xu
Objective To examine and talk about the mechanism of the Huoxue Jiegu compound capsule's effects on osteoblasts and the PI3K/Akt/mTOR signal pathway in rabbits suffering from tibial fractures. Method In vitro, CCK8 was used to assess the survival rates. Alizarinred staining was used to evaluate mineralized nodules. ALP staining was used to observe the osteoblasts. qRT-PCR was used to determine the mRNA expression of the bone formation-related factors BMP-2, bFGF, and TGF-β. In vivo, three groups of nine male rabbits each were randomly assigned to three groups: the Model group, the Huoxue Jiegu compound capsule group (HXJGC group), and the inhibitor group (HXJGC+3-MA), four weeks following the intervention. HE staining was employed to examine the rabbits' bone histology. immunohistochemistry was employed to examine the relative expression of the proteins VEGF and LC3-II. Western Blot was utilized to examine the relative expression of the proteins Beclin-1, LC3-II/Ⅰ, p62, p-PI3K, p-AKT, and p-mTOR. Results Compared to the control group, the medium- and high-dose groups exhibited considerably higher survival rates ( P < 0.05), as well as enhanced cell proliferation and differentiation ( P < 0.05) and more pronounced mineralized nodules. ( P < 0.05), but the low-dose groups showed no appreciable variation. In the low, medium, and high-dose groups, there was a substantial reduction in the expression of bFGF mRNA, whereas the levels of BMP-2 and TGF-β mRNA were considerably higher than in the control group ( P < 0.05). In vivo, after four weeks of treatment, the model control group and inhibito group had a large amount of fibrous hyperplasia accompanied by bleeding and a small amount of inflammatory cell infiltration. But in the HXJGC group, new cartilage appeared, and the surface of the cartilage was smooth and flat. Beclin-1 and LC3-II/I expression in the HXJGC+3 MA group was significantly lower than in the HXJGC and Model groups ( P < 0.05). The HXJGC group showed lower p62 expression than the HXJGC+3 MA and model groups ( P < 0.05). The HXJGC group exhibited significantly reduced levels of p-PI3K, p-AKT, and p-mTOR expression in comparison to HXJGC+3 MA groups ( P < 0.05). Conclusion Rabbits with tibial fractures can be treated with HXJGC, which can control the expression of the PI3K/Akt/mTOR signal pathway. It can promote the differentiation and maturation of osteoblasts at the fracture end of rabbits, accelerate the recovery of fractures, and achieve the purpose of treating the disease.
研究领域:
医学
·
生物学
✓ 已验证
DOI
PubMed
高影响因子
TOXICOLOGY IN VITRO
·
2025
·
IF 2.7
Chunlan Liang,Xuewei Xiong,Qi Shi,Qingqing Li,Guocheng Yu,Jingxiang Zhong,Lian Liu
Objectives This study aimed to investigate the effects and mechanisms of deoxynivalenol (DON) on rabbit corneal stroma cell culture and observe the protective effect of glycyrrhizic acid (GA) against DON-induced damage. Methods Rabbit corneal stromal cells (RCSCs) were isolated and divided into groups: control group (CON), 0.5 mmol/L GA (GA group), 400 ng/mL DON (DON group), and 0.5 mmol/L GA + 400 ng/mL DON (GAD). The effect of GA on RCSCs induced by DON was evaluated using various assays, including CCK-8 assay, Flow cytometry, qPCR, and Western blot. Results DON inhibited RCSCs proliferation, increased apoptosis, and raised the proportion of cells in the S and G2/M phase. It raised reactive oxygen species (ROS) levels and reduced mitochondrial membrane potential (MMP). Additionally, DON upregulated Bax, Caspase-3, inflammatory factors (IL-1α, IL-1β, TNF-α), and matrix metalloproteinases (MMP-1/8), while downregulating tissue inhibitors of matrix metalloproteinases (TIMP-1) and type I collagen. In contrast, GA partially restored RCSCs proliferation and reduced apoptosis, inflammation, and collagen degradation. Conclusions DON can induce toxicity in RCSCs, promoting the expression of inflammatory factors, disrupting the balance between MMP-1/8 and TIMP-1, and promoting type I collagen degradation. Conversely, GA reduces the oxidative stress damage, cell apoptosis, and inflammatory reaction of RCSCs induced by DON, and reduces the degradation of type I collagen.
研究领域:
药理学
·
毒理学
·
细胞生物学
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眼科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
·
2017
·
IF 4.159
Jinyu Li,Guoxin Tan,Bingchao Cheng,Dandan Liu,Weisan Pan
To facilitate the hydrophobic drugs modeled by coumarin-6 (Cou-6) acrossing the cornea to the anterior chamber of the rabbit eye, chitosan (CS) derivatives including chitosan-N-acetyl- l -cysteine (CS-NAC), chitosan oligosaccharides (COS) and carboxymethyl chitosan (CMCS) modified nanostructured lipid carriers (NLCs) were designed and characterized. We found that, with similar size distribution and positive charges, different CS derivatives based on NLCs led to distinctive delivery performance. In vivo precorneal retention study on rabbits revealed that these CS derivatives coating exhibited a stronger resistant effect than Cou-6 eye drops and Cou-6-NLC (P < 0.05), moreover, the AUC (0−∞) , C max and MRT (0−∞) of them followed the sequence of CMCS-Cou-6-NLC < COS-Cou-6-NLC < CS-NAC-Cou-6-NLC. Confocal laser fluorescence microscopy (CLSM) for in vitro corneal penetration study showed that COS-, and CS-NAC-coated NLCs penetrated through the whole corneal epithelium barrier (about 40 μm), while CMCS failed to significantly enhance the intraocular drug penetration as expected, displayed a negligible fluorescence at 30 μm deep. In addition, penetration through the intact cornea was achieved and the penetration levels through the ocular tissues were increased thoroughly for the COS and CS-NAC coating ones compared with CMCS-NLC (P < 0.05), and successfully reduced the conjunctival-to-corneal permeability ratio (ratio (C - S/C) ) thus resulted in a higher bioavailability, which was confirmed by ex vivo fluorescence imaging on ocular tissues. In summary, CS-NAC-NLC and COS-NLC are promising ocular drug delivery systems to achieve prolonged precorneal retention, higher corneal permeability and enhanced ocular bioavailability. And comparatively speaking, CS-NAC-NLC possesses the highest potential for ocular drug delivery.
研究领域:
药剂学
·
眼科研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jianxu Bao,Shifan Chen,Xianda Liu,Ziyue Ling,Chunji Jiang,Zhaoxi Han,Wenjie Wang,Ran Wei,Changsheng Zhao,Weifeng Zhao
Sepsis is a life-threatening systemic inflammatory response syndrome mainly caused by bacterial infection. Directly capturing live bacteria from blood could effectively avoid the activation of pathogen-associated molecular patterns. Despite the availability of numerous materials capable of capturing bacteria, most of these materials will negatively affect blood cells and thus exhibit poor blood compatibility. Given that bacteria are generally smaller than blood cells, we designed a fiber with a groove, where the groove width is larger than bacteria but smaller than blood cells, and trace amounts of positively charged copolymers are loaded into the grooves. This sophisticated design allows the grooved fibers to capture bacteria efficiently as well as maintain good blood compatibility. In vitro experiments show that bacteria could be trapped inside the groove and the fibers can remove over 98% of bacteria. In rabbits’ bacteremia model, these fibers significantly reduce bacterial concentration and endotoxin level, offering a promising therapeutic approach for the treatment of bacteremia. Groove shaped fiber exhibits excellent blood compatibility in both in vitro and in vivo environments. The developed groove shaped fiber opens a new pathway to design functional materials for extracorporeal blood purification.
研究领域:
生物医学工程
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材料科学
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感染性疾病研究
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产品关联
DOI
高影响因子
SENSORS
·
2017
·Vol. 17
·期 2
·221
·
IF 2.677
Wen-Shuai Jiang,Wei Xin,Shao-Nan Chen,Cun-Bo Li,Xiao-Guang Gao,Lei-Ting Pan,Zhi-Bo Liu,Jian-Guo Tian
Protein-protein interactions play an important role in the investigation of biomolecules. In this paper, we reported on the use of a reduced graphene oxide microshell (RGOM)-based optical biosensor for the determination of goat anti-rabbit IgG. The biosensor was prepared through a self-assembly of monolayers of monodisperse polystyrene microspheres, combined with a high-temperature reduction, in order to decorate the RGOM with rabbit IgG. The periodic microshells allowed a simpler functionalization and modification of RGOM with bioreceptor units, than reduced graphene oxide (RGO). With additional antibody-antigen binding, the RGOM-based biosensor achieved better real-time and label-free detection. The RGOM-based biosensor presented a more satisfactory response to goat anti-rabbit IgG than the RGO-based biosensor. This method is promising for immobilizing biomolecules on graphene surfaces and for the fabrication of biosensors with enhanced sensitivity.Keywords:reduced graphene oxide;microshells;optical sensor;goat anti-rabbit IgG;real-time;label-free
研究领域:
生物传感器
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纳米技术
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光学工程
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2013
·
IF 4.612
Xiupei Yang,Xiumei Cheng,Yingyun Lin,Zhijing Tan,Linxiang Xie,Martin M.F. Choi
This study reports a sweeping and micelle to solvent stacking (MSS) method for fast stacking of nitroimidazoles in capillary zone electrophoresis (CZE). The optimal experimental conditions are run buffer of 25 mM sodium phosphate (pH 1.5) and 0.50% (v/v) methanol, micellar solution of 30 mM sodium dodecyl sulfate, applied voltage of 28 kV, and sample and micellar solution injection of 100 s. By applying this new on-line preconcentration technique, the nitroimidazoles content can be determined within 9 min with the limit of detection ( S / N = 3) ranging 2.3–3.0 ng/mL, which is lower than that of conventional CZE analysis. The proposed MSS technique affords 20-, 12- and 38-fold improvements in sensitivity for the detection of dimetridazole, metronidazole and secnidazole, respectively. The relative standard deviations (RSDs) of intra-day and inter-day are 2.1–3.6% and 2.7–4.6% ( n = 6), respectively. The recoveries in pretreated rabbit plasma at spiked levels of 5.0–10.0 μg/mL are 92.0–101.1% with RSDs lower than 3.4%. The proposed sweeping and MSS-CZE is a highly sensitive method for the detection of nitroimidazoles in biological and clinical samples and has been successfully applied to analyze nitroimidazoles in pretreated rabbit plasma.
研究领域:
分析化学
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药物分析
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临床医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）