JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2018
·
IF 3.235
Mei Qianwen,Ding Yaping,Li Li,Wang Anqing,Duan Dingding,Zhao Yijun
Electrospun molybdenum disulfide (MoS 2 ) nanoparticles composite carbon nanofibers (MoS 2 -CNF) were first applied for determining the amount of Vanillin . The synthesized MoS 2 -CNF was characterized by scanning electron microscopy (SEM), Raman, X-Ray diffraction (XRD) and Electrochemical impedance technique (EIS). The electrochemical detection of vanillin was successfully conducted in 0.1 M Phosphate Buffer (pH 10) and showed a good linear response in the range of 0.3 to 135 μM and the detection limit reached 0.15 μM. The sensor showed a good amperometric response signal toward vanillin and result in the determination of real sample.
研究领域:
材料科学
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分析化学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Xiao-Yuan Wang,Jian Lv,Qin Hong,Ze-Rui Zhou,Da-Wei Li,Ruo-Can Qian
H2O2 is an essential signaling molecule in living cells that can cause direct damage to lipids, proteins, and DNA, resulting in cell membrane rupture. However, current studies mostly focus on probe-based sensing of intracellular H2O2, and these methods usually require sophisticated probe synthesis and instruments. In particular, local H2O2 treatment induces cell membrane rupture, but the level of cell membrane destruction is unknown because the mechanical properties of the cell membrane are difficult to accurately determine. Therefore, highly sensitive and label-free methods are required to measure and reflect mechanical changes in the cell membrane. Here, using an ultrasmall quartz nanopipette with a tip diameter less than 90 nm as a nanosensor, label-free and noninvasive electrochemical single-cell measurement is achieved for real-time monitoring of cell membrane rupture under H2O2 treatment. By spatially controlling the nanopipette tip to precisely approach a specific location on the membrane of a single living cell, stable cyclic membrane oscillations are observed under a constant direct current voltage. Specifically, upon nanopipette advancement, the mechanical status of the cell membrane can be sensibly displayed by continuous current versus time traces. The electrical signals are collected and processed, ultimately revealing the mechanical properties of the cell membrane and the degree of cell apoptosis. This nanopipette-based nanosensor paves the way for developing a facile, label-free, and noninvasive strategy to assay the mechanical properties of the cell membrane during external stimulation at the single-cell level.
研究领域:
生物医学工程
·
细胞生物学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
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IF 16.744
Junming Zhang,Yingyi Ma,Yuhang Han,Kaizheng Xu,Shun Yao,Lei Shi,Min Zhu
Restacking or aggregation inhibition of MXene nanosheets is crucial to the maintenance of their excellent electrochemical performance during use. In this study, a positively charged surfactant is inserted into the MXene nanolayers through a simple self-assembly process based on electrostatic interaction, producing a MXene/surfactant complex with enlarged interlayer spacing. Then 3D MXene-based porous structure could be prepared by the facile and low-cost breath figure method, in which MXene/surfactant complex is used as a casting material, therefore the inhibition of restacking or aggregation and increase of surface area at multiscale are realized in one system at the same time. Benefiting from the high conductivity of MXene and increased surface area of 3D porous structure that can provide massive active sites and facilitate the electrolyte infiltration and ion or electron access, enhanced electrochemical performance, for instance, electrochemical sensing of dopamine (DA) is shown. A low detection limit of 36.8 nM with excellent anti-interference, reproducibility, repeatability and long-term stability is realized. Good accuracy for DA detection in real biological samples is also confirmed by determining DA concentration in complex human urine samples. More importantly, porous structure such as pore size, uniformity, order and porosity can be adjusted through tuning the fabrication conditions, thereby regulating its electrochemical properties. This work not only provides a high-performance electrode for electrochemical sensing, but also offers a simple and practical strategy for the construction of 3D porous MXene film that holds great potential in electromagnetic interference shielding, supercapacitors and batteries.
研究领域:
材料科学
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电化学
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生物医学工程
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产品关联
DOI
高影响因子
ANALYST
·
2023
·Vol. 148
·期 4
·912-918
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IF 4.2
Xinai Zhang,Zhenzhong Wang,Xu Li,Wei Xiao,Xiaobo Zou,Qilin Huang,Lili Zhou
The development of effective methods for tracking cancer cells is of significant importance in the early diagnosis and treatment of tumor diseases. Compared with the developed techniques, the electrochemical assay has shown considerable potential for monitoring glycan expression on the cell surface using nondestructive means. However, the application expansion of the electrochemical strategy is strongly impeded owing to its dependence on electroactive species. In this study, a competitive electrochemical strategy was reported for monitoring cancer cells based on mannose (a typical glycan) as a clinical biomarker. Herein, functionalized carbon nanotubes were used to load the thiomannosyl dimer, and thionine-interlinking signal probes were designed for competitive recognition. After effective competition between cancer cells and the anchored mannose, a decreased current was obtained as the cell concentration increased. Under optimal conditions, the proposed biosensor exhibited attractive performance for cancer cell analysis with a detection limit as low as 20 cells per mL for QGY-7701 and 35 cells per mL for QGY-7703, facilitating great promise for the sensitive detection of cancer cells and thus showing potential applications in cancer diagnosis.
研究领域:
生物医学工程
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肿瘤学
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分析化学
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产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
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IF 1.5
Meng Jiang,Lin Zhu,Zejun Zhang,Yijing Ai,Lina Zeng,Shuhai He,Lin Li,Wei Sun
In this article the ZIF-8 based metal-organic frameworks-derived porous carbon was mixed with aminated graphene to obtain the nanocomposite, which was used to modify the surface of glassy carbon electrode (GCE). SEM, XRD and FT-IR were used to characterize the nanocomposite, which exhibited the unique morphology with functional groups. The modified electrode was applied to the electrochemical detection of rutin. Compared with that of GCE, the current response of rutin increased obviously on the modified electrode. Square wave voltammetry was further applied to quantitative analysis of rutin with a linear relationship between the concentration and peak current in two sections from 0.25 to 0.7 μmol·L -1 and 0.7 to 1.5 μmol·L -1 with detection limit of 15 nmol∙L -1 . Consequently, the detection of real samples was realized by this method.
研究领域:
材料科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2023
·Vol. 148
·期 19
·4869-4876
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IF 4.2
Yixin Pan,Jing Jiang,Xianwen Kan
Development of an effective and convenient sensor for sensitive detection of nitrites is of great concern since excessive amounts of nitrites can be harmful to both human health and the environment. In this work, Cu-MOF modified exfoliated graphite paper (EGP) was employed as a signal reporter to enable the visual and electrochemical dual-mode sensing of nitrites. Cu-MOFs were in situ synthesized on EGP, which exhibited an excellent oxidase enzyme-like activity to oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) into its oxidation product (oxTMB). The multi-layer structure and the superior electrical conductivity of EGP not only facilitated the loading of the Cu-MOF nanozyme for colorimetric sensing but also enabled its use as an underlying backbone to support electroanalysis. Based on the recognition of nitrite through a highly specific diazo reaction between nitrite and oxTMB, the addition of nitrite caused the colorimetric sensing solution to change color from blue to green, which allowed for the colorimetric sensing of nitrite with a limit of detection (LOD) of 8.5 × 10−6 mol L−1. Meanwhile, the Cu-MOF/EGP electrochemical platform was employed for ratiometric detection of nitrite based on the electrochemical oxidation of nitrite and TMB. Compared with the colorimetric mode, the electrochemical mode possessed higher sensitivity with a LOD of 5.4 × 10−7 mol L−1, indicating the high sensitivity and accuracy of the proposed dual-mode sensing strategy. Furthermore, the determination of nitrite in different pickled food samples is demonstrated.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
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IF 2.7
Weiqiang Li,Xiaoxue Liu,Pianpian He,Wentao Hu,Kaijie Tang,Yangping Wen,Qian Zeng,Huiyi Tang,Yaxuan Lei,Xin Liu
Deoxynivalenol (DON) has drawn considerable attention for its obvious pathogenicity and wide use in agro-products, which cause a potential threat to human health. In this work, an electrochemical immunosensor is developed for the highly sensitive and selective detection of DON in wheat flour using AuNPs-BP-MWCNTs-COOH and antibodies. The AuNPs-BP-MWCNTs-COOH nanocomposite was prepared via an in situ reduction reaction and ultrasonic-assisted liquid-phase exfoliation. The nanocomposite exhibits a larger surface area, decent stability, excellent electron transfer capability, good protein binding capability and prominent specificity. The plentiful carboxyl group on the nanocomposite can bind to the amino group of the antibody, and AuNPs have an affinity for the sulfhydryl group of the antibody, which makes it feasible for the nanocomposite to load the antibody. The peak currents are plotted against the logarithm of DON concentration from 0.002 to 80 ng mL−1 with a limit of detection (LOD) of 0.5 pg mL−1. This approach establishes an effective label-free immunosensor platform for the detection of DON with high sensitivity and selectivity in various food and agricultural products.
研究领域:
食品安全
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分析化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2024
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IF 2.7
Jiaqi Fu,Jincheng Du,Ting Hou,Zhong Wang,Liyuan Sui,Jinlian Li,Yanli Zhao,Ximing Cui,Dongmei Wu
Electrochemistry with purines as indicators may be a promising new method for antibiotic activity testing. However, the traditional electrochemical detection method (S-LSV) requires a long accumulation time for purines and has low signal intensity. High-temperature treatment of bacterial samples may significantly enhance the signal intensity and speed of detection. However, this process may also impact the structure and metabolism of bacteria. Further research is necessary to determine how electrochemical signals respond to antibiotic antibacterial activity. In this paper, the electrochemical detection method based on heat-treated Escherichia coli ( E. coli , H-LSV) was used to investigate the effects of 11 antibiotics with four different antibacterial mechanisms on the electrochemical behavior of E. coli . The results showed that compared to the S-LSV, the electrochemical signal of E. coli detected by the H-LSV increased by 362%, and showed a strong linear relationship with bacterial counts up to 1.88×10 8 CFU/mL, with a detection limit of 1.15×10 6 CFU/mL. The electrochemical signal consistently correlated with changes in viable E. coli counts following treatment with the four different antibacterial mechanisms. HPLC analysis further confirmed that the changes in purine contents in bacteria after antibiotic treatment were consistent with the trends in electrochemical signals. The evaluation of antibiotic antibacterial ability by H-LSV showed good consistency with results from plate counting and turbidity methods, but was closer to the results of plate counting. The inhibition rates of antibiotics on E. coli detected by H-LSV and S-LSV also showed high consistency, indicating that short-term high-temperature treatment of E. coli did not affect the response of the bacterium to the inhibitory activity of antibiotics.
研究领域:
微生物学
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药理学
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分析化学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Wenting Cheng,Han Pan,Jinhua Chen,Miao He,Zhongyun Wang,Yang Xiang
Exosomal miRNAs, particularly miRNA-21, are promising cancer biomarkers. Current enzyme-dependent detection methods face challenges, such as environmental limitations and high costs. In contrast, enzyme-independent sensors are highly desirable for on-site, miniaturized, and cost-effective miRNA detection. To address these limitations, we developed a nonenzymatic electrochemical sensor featuring a triple-signal amplification system for ultrasensitive detection of miRNA-21. This sensor utilizes cascade toehold-mediated strand displacement reactions to activate molecular machines triggered by target miRNA, generating biotinylated-and-thiol-modified double-stranded DNA for stable immobilization on a gold electrode. Preprepared biotinylated tetrahedron DNA (TDNA)-mediated hybridization chain reaction probes are then linked to the electrode via streptavidin–biotin binding. This amplification process allows for significant DNA duplex immobilization, with electroactive [Ru(NH3)6]3+ (RuHex) adsorbed onto them, producing a robust electrochemical signal. This approach enables accurate detection of miRNA-21 at concentrations as low as 0.43 fM, with a linear range from 1 fM to 1 nM. Clinical testing demonstrates its potential for cancer diagnostics.
研究领域:
生物化学
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医学诊断
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Liu Shi,Lin Wang,Xuemei Ma,Xiaona Fang,Liangliang Xiang,Yongxiang Yi,Jinlong Li,Zhaofeng Luo,Genxi Li
With the outbreak of COVID-19, which is fast transmitting and highly contagious, the development of rapid, highly specific, and sensitive detection kits has become a research hotspot. The existing assay methods for SARS-CoV-2 are mainly based on enzymatic reactions, which require expensive reagents, hindering popular use, especially in resource-constrained areas. Herein, we propose an aptamer-based method for the assay of SARS-CoV-2 via binding of the spike protein using functionalized biomimetic nanochannels. To get the analogous effect of human ACE2, a receptor for the spike protein, the aptamer to bind to the spike S1 protein has been first screened by a SELEX technique and then immobilized on the previously prepared nanochannels. In the presence of SARS-CoV-2, the changes in steric hindrance and charge density on the surface of the nanochannels will affect the ion transport, along with a rapid electrochemical response. Our method has been successfully applied to detect the viral particles in clinical pharyngeal swab specimens in one step without sample treatment. We expect this rapid, reagent-free, and sensitive assay method to be developed as a useful tool for diagnosing COVID-19.
研究领域:
生物医学工程
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病毒学
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诊断学
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 5
·1-9
·
IF 5.3
Zhang Guiyun,Lai Zhiwei,Xie Jianhua,Yang Weiqiang
Methylmercury (CH 3 Hg + ), a lipophilic environmental pollutant, accumulates in fish, shellfish, and other organisms, posing significant risks to human health through the food chain. Developing a convenient and sensitive analytical method for CH 3 Hg + detection is crucial for reducing costs and enhancing the efficiency of food safety testing. In this study, we prepared an octyl-modified silica isoporous membrane on the indium tin oxide (ITO) electrode (Octyl-SIM/ITO) via the electrochemical-assisted self-assembly (EASA) method using octyltrimethoxysilane (O-TES) as the functional organosilane. The Octyl-SIM/ITO electrode exhibits vertically-ordered nanochannels and strong hydrophobic affinity, enabling selective penetration and enrichment of weakly polar analytes. Utilizing square wave anodic stripping voltammetry (SWASV), the Octyl-SIM/ITO electrode demonstrates superior electrochemical response signals for CH 3 Hg + detection, achieving a detection limit as low as 4 nM. This method allows for accurate and reproducible detection of CH 3 Hg + in fish and oyster samples with minimal sample preparation, offering promising potential for portable in situ detection. Graphical
研究领域:
环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Yiwei Sun,Yuting Luo,Haoxiang Wang,Yuyang He,Ruoyu Wang,Mengqi Lu,Dongpo Xu
An efficient electrochemical molecularly imprinted sensor for the detection of tetracycline (TC) was prepared using ZnO/MIP. ZnO nanomaterials with large surface area improved the electrochemical detection performance and also increased the binding sites of the TC molecularly imprinted polymeric membrane. The TC molecularly imprinted polymeric membrane with pyrrole as the functional monomer specifically binds to TC and thus detects TC in the sample. In this study, the electrochemical performance of ZnO/MIP was evaluated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Under the optimal conditions, ZnO/MIP showed a wide detection range. The detection range was 0.1–100 nM, and the detection limit was 0.02 nM with R2 reaching 0.9974. ZnO/MIP has good selectivity, stability, and reproducibility. The recovery of tetracycline in milk was 97–112%.
研究领域:
分析化学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）