Journal of Cleaner Production
·
2022
·
IF 11.072
Xu-Hui Huang,Rong Liu,Jia-Nan Chen,Xiu-Ping Dong,Bei-Wei Zhu,Lei Qin
Erythromycin has caused severe risk on the aquatic ecological environment and food chain propagation due to its extensive use, environmental persistence, and low efficiency of sewage removal. Our study monitored the erythromycin accumulation, self-detoxification behavior, and metabolic response of turbot in erythromycin-contaminated environments using a multi-omics method with the aim to improve the safety of turbot consumption and provide guidance for turbot culture. Erythromycin was found to rapidly accumulated and reached its peak in turbot in the first two days of exposure to erythromycin. Residual levels in the gill, liver, kidney, muscle, and blood were detected at 95.19, 72.80, 68.74, 51.3, and 12.33 ng/g, respectively. The risk of erythromycin residues in turbot can be effectively reduced by a withdrawal period longer than 12 d. Erythromycin-contaminated turbot muscles showed a significant decrease in flavor properties, particularly umami, sweet, and aroma precursors. A total of 232 lipids in four main categories, glycerides, phospholipids , fatty acyl, and sphingolipids, were found to show significant changes in the turbot muscles. The interacting metabolic network formed by taurine, glycerophospholipid, arginine, alanine , and glutamate metabolism, wherein turbot responded to erythromycin was verified through 14 significantly altered metabolites. A withdrawal period of at least half a month and the corresponding product labels for antibiotics should be formulated before the fish exposed to antibiotics during intensive culture are sold to protect the interests of consumers. This study could serve as a reference for the quality and safety risk assessment of turbot and other highly economical fishes affected by antibiotics exposure.
研究领域:
环境科学
·
水产养殖学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 15
·3489
·
IF 4.2
Yanxiang Zhang,Chunxing Li,Xinyu Zhu,Irini Angelidaki
High-level erythromycin (ERY) fermentation wastewater will pose serious threats to lake environments. Anaerobic digestion (AD) has advantages in treating high-level antibiotic wastewater. However, the fate of antibiotic resistance genes (ARGs) and microbial communities in AD after stepwise exposure to high-level ERY remains unclear. In this study, an AD reactor was first exposed to 0, 5, 10, 50, 100 and 200 mg/L ERY and then re-exposed to 0, 50, 200 and 500 mg/L ERY to investigate the effect of ERY on AD. The results show that AD could adapt to the presence of high-level ERY (500 mg/L) and could maintain efficient CH4production after domestication with low-level ERY (50 mg/L). The AD process could achieve higher removal of ERY (>94%), regardless of the initial ERY concentration.ErmBandmefA, conferring resistance through target alteration and efflux pumps, respectively, were dominant in the AD process. The first exposure to ERY stimulated an increase in the total ARG abundance, while the AD process seemed to discourage ARG maintenance following re-exposure to ERY. ERY inhibited the process of acetoclastic methanogenesis, but strengthened the process of hydrogenotrophic methanogenesis. This work provides useful information for treating high-level ERY fermentation wastewater by the AD process.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Liang Hu,Anle Ge,Xixian Wang,Shanshan Wang,Xinpei Yue,Jie Wang,Xiaojun Feng,Wei Du,Bi-Feng Liu
Immune response to environmental pathogen invasion is a complex process to prevent host from further damage. For quantitatively understanding immune responses and revealing the pathogenic environmental information, real-time monitoring of such a whole dynamic process with single-animal resolution in well-defined environments is highly desired. In this work, an integrated microfluidic device coupled with worm-based biosensor was proposed for in vivo studies of dynamic immune responses and antibiotics interference in infected C. elegans . Individual worms housed in chambers were exposed to the various pathogens and discontinuously manipulated for imaging with limited influence on physiological activities. The expression of immune responses gene ( irg-1 ) was time-lapse measured in intact worms during pathogen infection. Results demonstrated that irg-1 gene could be induced in the presence of P. aeruginosa strain PA14 in a dose-dependent manner, and the survival of infected worm could be rescued under gentamicin or erythromycin treatments. We expect it to be a versatile platform to facilitate future studies on pathogenesis researches and rapid drug screen using C. elegans disease model.
研究领域:
生物学
·
医学
·
药物研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yulei Zhang,Zhaolin Huang,Wenli Zhang,Dawei Ni,Wei Xu,Cuie Guang,Wanmeng Mu
The presence of macrolides in food and the environment poses a substantial threat to the sustainable development of natural ecosystems and presents risks to human health. Previous research has demonstrated that microbial esterases can degrade erythromycin (Ery), roxithromycin (Rox), and clarithromycin (Clr), rendering them candidates for bioremediation of macrolide residues. However, the efficacy of esterases is limited by insufficient thermal stability, restricting their widespread application. To address this challenge, this study employed a combinatorial strategy integrating computational molecular dynamics (MD) simulations, server-assisted virtual mutation prediction, and a greedy algorithm for protein engineering to enhance the thermal stability of esterase C from Enterobacter hormaechei ( Ehno -EreC). After four rounds of screening, we successfully obtained a four-point mutant, M4 (D245P/S141A/D154I/T292W), which exhibited significantly improved thermal stability compared to the wild-type enzyme. Notably, the M4 mutant not only extended the enzyme's half-life at 40 °C from 7 to 1095 min, but also increased its degradation activity against Ery, Rox, and Clr. Structural analyses revealed enhanced rigidity in the loop between the major and minor lobes and the strengthened hydrophobic interactions post-mutation, contributing to the enzyme's structural stability. This finding was consistent with the observed reduction in correlated and anti-correlated motions in the MD trajectory.
研究领域:
生物化学
·
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
VETERINARY MICROBIOLOGY
·
2023
·
IF 3.3
Yumeng Cheng,Yakun Li,Meng Yang,Yang He,Xinru Shi,Zhidan Zhang,Yesheng Zhong,Yuan Zhang,Hongbin Si
Antibiotic-resistant bacteria are emerging all the time, but the continued emergence of novel resistance genes and genetic structures is even more alarming. Tigecycline is currently the important last barrier in the treatment of multidrug-resistant (MDR) infections. tet (X), a resistance gene to tigecycline, is the most prevalent and constantly emerging novel variants. In this research, we characterized two MDR Acinetobacter indicus strains to tigecycline that were identified and analyzed by antimicrobial susceptibility testing, conjugation transfer, whole genome sequencing (WGS) and bioinformatics analysis, and gene function analysis. The results showed that three tet (X) variants were carried in BDT201, including tet (X6) on the chromosome, tet (X3) on the plasmid pBDT201–2, and a novel tet (X5) variant adjacent to the IS Aba1 elements on the plasmid pBDT201–3. The novel Tet(X5) variant showed 98.7% amino acid identity with Tet(X5) and was named Tet(X5.4). By expressing tet (X5.4) gene, the tigecycline minimum inhibitory concentration (MIC) values for Escherichia coli JM109 increased 32- fold (from 0.13 to 4 mg/L). BDT2076 contained tigecycline and carbapenems resistance genes, such as tet (X3), bla OXA-58 , bla NDM-3 , and bla CARB-2 . The continuous emergence of MDR bacteria and resistance genes is a global environmental health issue that can not be ignored and therefore needs to pay more urgent attention to it.
研究领域:
微生物学
·
分子生物学
·
抗生素研究
·
环境健康
✓ 已验证
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Yanxiang Zhang,Huiling Liu,Xiaohu Dai,Chen Cai,Jing Wang,Yunpeng Shen,Peng Wang
Erythromycin mycelial dreg (EMD) resulting from erythromycin (ERY) production process may be used as a nutrient resource. However, it has a high moisture content, which makes handling operations complicated. In this study, the EMD dewaterability under heat-activated persulfate (PS) oxidation conditioning was investigated. The variations of physical and chemical properties of EMD were further investigated to uncover the underlying mechanism of EMD dewaterability. Results indicated that EMD dewaterability was worse under single heat process, but significantly improved under heat-activated PS oxidation process. Heat-activated PS oxidation conditioning was capable to increase zeta potential , reduce median particle size, alter EMD flocs morphology, and disrupt mycelial cells. Concurrently, heat-activated PS oxidation conditioning resulted in the decrease of protein in tightly bound (TB) extracellular polymeric substances (EPS) and total EPS, and the decrease of fluorescing constituents (tryptophan protein, tyrosine protein and tryptophan amino acid) in EPS. Furthermore, a possible mechanism was proposed for heat-activated PS oxidation conditioning.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Peng Zhang,Guanghua Lu,Yu Sun,Zhenhua Yan,Tianjian Dang,Jianchao Liu
The aging process changes the physicochemical structure of microplastics and affects environmental behaviors and toxicological effects of coexisting pollutants, thereby posing ecological risks. In this study, the effects of aged polystyrene microplastics alone or in combination with the 100 μg/L roxithromycin (ROX) on intestines of Carassius auratus were investigated. The carrier effect of microplastics was enhanced by aging due to changes in functional groups and surface area, which led to an increase in the bioaccumulation of ROX. The combined exposure of aged microplastics (APS) and ROX caused more inflammatory cell infiltration and cilia defects, and significantly inhibited the activity of amylase and lipase. Metagenomic sequencing revealed that the combined exposure of microplastics and ROX increased the abundance of Gemmobacter , Bosea , Rhizobium , and Shinella and decreased the abundance of Cetobacterium and Akkermansia ( p < 0.05). The presence of APS enhanced the selective enrichment of antibiotic resistance genes. What's more, the influence of microplastics and antibiotics on gut microbiota was closely related to carbohydrate metabolism and amino acid metabolism activities, as well as the abundance of baca and sul1 resistance genes. These results expand our understanding of the interaction mechanism between APS and antibiotics in real aquatic environment.
研究领域:
环境科学
·
微生物学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Na-Na Li,Xing-Yu Xu,Ping Qiu,Yong-X Li,Xiong-Sheng Yu,Yong-E Gao,Hai-Xian Ren,Mohd. Muddassir,Wan-Ying Lin,Zi-Ao Zong
Erythromycin could be used to treat various bacterial infection, but it was harmful to the colonic microflora. Therefore, it is highly desirable to develop a fluorescence probe that could selectively and sensitively detect Erythromycin in pure water. In this work, a fluorescent probe named EHMC , which exhibited aggregation-induced emission (AIE) characteristic in solid state and water/EtOH binary solvent was developed for “turn on” sensing Erythromycin in pure water with high selectivity and sensitivity (detection limit: 1.78 × 10 −8 M). Also, there are fewer interference from other antibiotics in the detection process of probe EHMC for Erythromycin. Moreover, probe EHMC could as a portable test strips for highly selective detection of Erythromycin and identification of different concentrations of Erythromycin. In addition, living cells imaging experiments displayed that probe EHMC could detect Erythromycin in A549 cells and BEAS-2B cells successfully. Combined with the theoretical calculation results The sensing mechanisms that the C O in Erythromycin and OH in EHMC formed intermolecular hydrogen bond and further formed new aggregates were confirmed by job’ plot, 1 H NMR, FT-IR, ESI-MS, DLS and TEM and DFT calculation.
研究领域:
生物化学
·
医学检测
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Jinyun Peng,Qing Huang,Wenfeng Zhuge,Yuxia Liu,Cuizong Zhang,Wei Yang,Gang Xiang
In this study, we developed a novel photoelectrochemical (PEC) sensor for the highly sensitive detection of erythromycin by functionalising graphene oxide (GO) with nickel tetra-amined phthalocyanine (NiTAPc) through covalent bonding, which resulted in the formation of NiTAPc-Gr. The fabricated sensor showed a higher PEC efficiency under blue light, exhibiting a peak wavelength of 456 nm, as compared to that of the monomer . Further, the NiTAPc-Gr/indium tin oxide (ITO) sensor exhibited a photocurrent that was 50-fold higher than that for a GO/ITO sensor under the same conditions. Under optimal conditions, the NiTAPc-Gr PEC sensor showed a linear response for erythromycin concentrations ranging from 0.40 to 120.00 μmol L −1 , with the minimum limit for detection being 0.08 μmol L −1 . Thus, the NiTAPc-Gr sensor exhibited superior performance and excellent PEC characteristics, high stability, and good reproducibility with respect to the sensing of erythromycin. Moreover, it is convenient to use, fast, small, and cheap to produce. Hence, it should find wide use in the analysis of erythromycin in real-world applications.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2023
·Vol. 34
·期 1
·1-13
·
IF 2.8
Lai Tingrun,Shu Hui,Tian Xu,Ren Jie,Cui Xiuxiu,Bai Huiping,Xiao Xuechun C.,Wang Yude D.
As a macrolide antibiotic, erythromycin is often used to treat bacterial infections in animal husbandry, but excessive use of erythromycin will make its residue in the animal and subsequently enter the human body through consumption, furthermore causing a different degree of damage to human health. It is quite essential to develop a fast and precise quantitative detection manner for erythromycin. In this study, we use arginine as a functional monomer and erythromycin as a template molecule to electro-polymerize a layer of molecularly imprinted polymer film on the surface of a glassy carbon electrode, and then use it as a recognition element to achieve quantitative erythromycin monitoring. The linear detection range of the prepared electrochemical sensor is from 3 nM to 1600 µM, the lowest detection limit ( S/N = 3) is 2.01 nM, and the sensitivity is 57,300 µA cm −2 µM −1 . The selectivity, reproducibility, and stability of the electrochemical sensor are satisfactory. At the same time, it can monitor whether honey contains erythromycin and its concentration, the recovery rate from 96.4 to 104.2%. After a series of performance tests, the results indicated that the Arg-MIP/GCE sensor has the potential to be applied in the measurement of erythromycin, which exists in food and agro-products.
研究领域:
分析化学
·
食品科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2023
·
IF 4.7
Donghao Yuan,Shidong Wang,Xue Li,Muzi Zhang,Ming Li
As an inevitable factor in aquaculture, ammonia plays a critical role in macrolide antibiotic resistance, leading to accumulating of antibiotic-resistant bacteria in fish skin mucus. In this study, four experimental groups were implemented to test the effects of ammonia alone or in combination with roxithromycin for 28 days on skin mucus microbial composition and the immune response of yellow catfish: CON (control), AN (50.00 mg L −1 total ammonia nitrogen, TA-N), ROX (100 μg L −1 roxithromycin), and HR (50.00 mg L −1 TA-N, 100 μg L −1 ROX). This study demonstrated that ammonia or roxithromycin exposure resulted in increased plasma ammonia content and decreased total antioxidant capacity. Compared with AN group, the combined exposure of ammonia and roxithromycin inhibited the skin mucus immune response. Microbial composition analysis showed that combined exposure of ammonia and roxithromycin had no significant effect on skin mucus α-diversity as compared with CON group. The abundance of Cetobacterium , Rhizobiales_Incertae_Sedis_uncultured and Acinetobacter was increased significantly with the combined effect of ammonia and roxithromycin, these bacteria may be potentially antibiotic-resistant. As compared with CON group, the combined exposure of ammonia and roxithromycin did not affect skin goblet cell counts. This study suggests that combined exposure to ammonia and ROX increases the risk of the emergence of antibiotic-resistant bacteria.
研究领域:
水产养殖
·
微生物学
·
免疫学
✓ 已验证
DOI
PubMed
高影响因子
Surfaces and Interfaces
·
2025
·
IF 6.3
Di Wang,Geng Chen,Jinze Li,Yumeng Ren,Yulin Zhao,Wensheng Yang,Yingying Zhu
Elemental doping is a widely adopted strategy for optimizing the electronic structure of carbon nitride and improving its photocatalytic performance. However, single-element doping offers limited enhancement. In this study, phosphorus and molybdenum co-doped carbon nitride (P-Mo-C₃N₄) was synthesized by wet ball-milling, a simple and scalable method. The co-doping of P and Mo synergistically modulates the band structure of g-C₃N₄, significantly enhancing its redox capability, while their compatibility effectively reduces interfacial resistance. In Rhodamine B (RhB) degradation tests, P-Mo-C₃N₄ achieved a first-order rate constant ( k ) of 0.1065 min –1 , surpassing those with g-C₃N₄, P-C₃N₄, and Mo-C₃N₄ by factors of 10.2, 6.5, and 8.6, respectively. Furthermore, P-Mo-C₃N₄ exhibited excellent degradation performances towards tetracycline hydrochloride (TC) and methylene blue (MB). After five cycles, the catalyst retained 90 % efficiency, demonstrating excellent stability. This work provides a cost-effective and eco-friendly approach for developing advanced C₃N₄-based photocatalysts.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）