SEPARATION AND PURIFICATION TECHNOLOGY
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2022
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IF 9.136
Xiaohui Zhan,Junyan Liu,Yue Zhao,Yanping Sun,Rui Gao,He Wang,Huixiang Shi
In this study, we designed and synthesized Mn III with tunable spin state in g-C 3 N 4 through one-step calcination of Mn-MOF. The Mn atoms were induced into the six-fold cavity of the g-C 3 N 4 lattice and formed Mn-N 6 coordination. Mn-MOF served as the source of Mn. The collapse of Mn-MOF effectively promoted the stripping of stacked layers in g-C 3 N 4 and increased the atomic loading. The Mn-N coordination induced impurity energy levels which effectively regulated Mnx-CN's bandgap structure and accelerated the migration and separation of carriers. The O 2 − was activated to 1 O 2 by the energy released from the high-spin Mn III transferred ground state. The H 2 production of Mn 10 -CN (13479.19 µmol·g −1 ·h −1 ) is ∼32.2 times that of CN under visible light irradiation and with a remarkable apparent quantum yield (AQY) of 12.23% at 420 nm. The rates of TC degradation and Cr (VI) reduction achieved 0.059 and 0.18 min −1 , respectively. In addition, the practical application conditions and catalytic mechanism of Mnx-CN were explored. This work demonstrates the connection of the spin state of metal with the photocatalytic activity, providing an efficient photocatalytic system that can be used for solar-chemical energy conversion and pollution remediation, and presenting a new approach for modifying the g-C 3 N 4 -based catalysts.
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FOOD CHEMISTRY
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2022
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IF 9.231
Xun Zhou,Yongxiang Wu,Yao Jiang,Chen Li,Longping Xu,Peng Cui,Xinsong She
Integrated one–dimensional CuO–nanowire arrays/Cu–foam (CuO–NWAs/Cu–foam) nanostructure, which was fabricated by oxidation and calcination, has been newly utilized as non–enzymatic electrocatalytic electrode for exploring histamine level. Under optimal condition of pH at 13 and potential at 0.55 V ( vs Ag/Ag/Cl), the sensitivity of CuO–NWAs/Cu–foam electrode towards non–enzymatic electrochemical histamine determination presented as high as 12.94 mA mM −1 cm −2 , linear range spanned between 0.5 and 1046 μM, a detection limit (S/N = 3) was about 44 nM. The unprecedented causes of ultrahigh sensitivity were physically contributed to enhancing active surface area and declining charge transfer resistance. More crucially, the outstanding selectivity, stability and reproducibility facilitated its practical capacity on evaluating histamine levels in different–bacteria fermented mandarin fish, which triggered the potential feasibility for commercializing non–enzymatic electrochemical determination of histamine with high sensitivity, reliable precision and low expenditure.
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FOOD CHEMISTRY
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2022
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IF 9.231
Yuqing Qin,Weikang Ke,Aroosha Faheem,Yueyun Ye,Yonggang Hu
Due to growing food safety issues, developing economic, rapid, and sensitive strategies for food spoilage monitoring has attracted significant attention. Here, a Bacillus subtilis spore-based biosensor is presented for rapid, highly sensitive, visual biogenic amines detection. The biosensor is fabricated through biogenic amines-induced pH increase which inhibits the electron transfer between Cu ion sites within CotA-laccase on the spore surface, leading to decrease in catalytic oxidation activity towards the chromogenic substrates. The developed system integrated with smartphone analysis realized the on-site monitoring of histamine with a detection range of 0.17–120 mg L −1 , and a detection limit of 0.17 mg L −1 (3σ). Moreover, the color change induced by histamine is observable by the naked eye. The smart biosensor was successfully applied for food freshness evaluation in raw meat samples, showing several advantages, including eco-friendliness, low cost, and high stability, meeting the demands of on-site monitoring in the food safety field.
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Foods
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2022
·Vol. 11
·期 20
·3306
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IF 5.561
Xiaofu Wang,Yunsong Zhao,Sufang Zhang,Xinping Lin,Huipeng Liang,Yingxi Chen,Chaofan Ji
Biogenic amines (BAs) are produced by microbial decarboxylation in various foods. Histamine and tyramine are recognized as the most toxic of all BAs. Applying degrading amine enzymes such as multicopper oxidase (MCO) is considered an effective method to reduce BAs in food systems. This study analyzed the characterization of heterologously expressed MCO fromL. sakeiLS. Towards the typical substrate 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), the optimal temperature and pH for recombinant MCO (rMCO) were 25 °C and 3.0, respectively, with the specific enzyme activity of 1.27 U/mg. Then, the effect of different environmental factors on the degrading activity of MCO towards two kinds of BAs was investigated. The degradation activity of rMCO is independent of exogenous copper and mediators. Additionally, the oxidation ability of rMCO was improved for histamine and tyramine with an increased NaCl concentration. Several food matrices could influence the amine-oxidizing activity of rMCO. Although the histamine-degrading activities of rMCO were affected, this enzyme reached a degradation rate of 28.1% in the presence of surimi. Grape juice improved the tyramine degradation activity of rMCO by up to 31.18%. These characteristics of rMCO indicate that this enzyme would be a good candidate for degrading toxic biogenic amines in food systems.Keywords:Lactobacillus sakei;biodegradation;multicopper oxidase;food matrices;biogenic amines
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JOURNAL OF PHARMACY AND PHARMACOLOGY
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2022
·Vol. 74
·期 9
·1241-1250
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IF 4.81
Huang Wenwen,Zhou Xiaoying
ObjectivesTo examine the possible anti-histamine effects of dipotassium glycyrrhizinate (DG), a dipotassium salt of glycyrrhizic acid, on histamine-mediated lung fibroblast activation, differentiation and proliferation; to investigate the potential and underlying mechanisms for pulmonary fibrosis (PF) treatment.MethodsRat primary lung fibroblasts were extracted to establish cell models; histamine, DG and loratadine (LTD, a histamine receptor antagonist) were applied. Cell proliferation, migration and cell cycle were explored; intracellular signal proteins were detected; mitochondrial membrane potential was examined.Key findingsThe anti-histamine effects of DG were found in a similar pattern of LTD on lung fibroblasts. DG inhibited histamine-induced cell activation, proliferation and migration; DG altered histamine-mediated mitochondrial membrane potentials. DG reduced the histamine-induced PAR-2 (a tryptase receptor) expression to impair mast cell tryptase co-working. Histamine-induced expressions of MMP-2, FAK, TNF-α, P38, iNOS were decreased by DG, while Bax and caspase-3, P53 were increased by DG against histamine effects. Histamine drove cells from G0/G1 to S phases, whereas DG rested cells by inhibiting G0/G1 and G2/M phases.ConclusionsThis study provided the evidences that DG can inhibit histamine-induced effects on lung fibroblasts and promote apoptosis of abnormally activated lung fibroblasts, implicating its potential therapeutic mechanisms against PF development, also for those histamine-related diseases.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Jianfeng Yan,Quanbin Fu,Shikai Zhang,Yu Liu,Xianbao Shi,Juying Hou,Junling Duan,Shiyun Ai
A novel dual-emission ratiometric fluorescent sensor for biogenic amines (BAs) was prepared by simple mixing blue fluorescent carbon dots (CDs) and yellow fluorescent CdTe quantum dots (CdTe QDs). Based on different sensitive properties of pH, CdTe QDs and CDs were used as the response signal and internal reference signal, respectively. The developed ratiometric fluorescent sensor achieved quantitative analysis of eight kinds of BAs with rapid response (30 s) and low limits of detection (1.259–5.428 μM). Furthermore, color-tunable fluorescent test strips were constructed by easily assembling CDs and CdTe QDs onto filter paper. The obtained smart label showed a distinguishable fluorescent color variation from blue to green during the corruption of shrimp samples. The smart label with advantages of convenience and rapidness provided a method for visually monitoring the freshness of food samples.
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SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Yujuan Qin,Pengcheng Huang,Fang-Ying Wu
With food safety problems abounding, it is essential to explore some efficient and effective methods for monitoring food quality. To this end, we have developed a histamine-responsive carbon dots (CDs)-based sensing system for visual monitoring of food spoilage. In this system, the aldehyde group on the surface of CDs serves as the recognition sites to combine with the amino group on histamine to form a Schiff base that effectively enhances the fluorescence. On the other hand, 1-aminoanthraquinone (ANQ) was introduced as a reference signal to endow the fluorescent probe with self-calibrating capacity. As the histamine concentration increases, linear ratiometric response was obtained accompanied with a distinguishable fluorescence color change from red to blue. Besides, test strips-based smartphone visual assays were successfully used to evaluate and monitor the histamine levels in fish samples. Our proposed sensing strategy has been demonstrated to be efficient and robust along with great potential for real-time visual monitoring of food spoilage.
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FOOD CONTROL
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2022
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IF 6.652
Sihan Zhang,Qinzhen Fan,Jiaqi Guo,Xianhe Jiao,Xianming Kong,Qian Yu
Sensitive and rapid detection of histamine in fish sample was important for human health. A novel and facile method for on-site detecting histamine in fish was developed by tandem surface-enhanced Raman spectroscopy (SERS) with derivatized thin-layer chromatography. Histamine in tuna was firstly separated by TLC process on a diatomite plate, and then derivatized with fluorescamine (FSA). SERS is used for identification of histamine derivative by a portable Raman spectrometer. The operating conditions were optimized for improving the detection performance. Diatomite plate was suitable for separation and derivatization, 40 times concentrated Au colloid, 1 mM of FSA at pH = 8 were appropriate for the derivatization and SERS detection, and the limit of detection of histamine could achieve 9 ppb. The results indicated that the proposed method not only visualized the histamine on TLC plate but also improve the sensitivity in SERS, that could be employed for the on-site separating and detecting histamine from fish sample.
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JOURNAL OF CHROMATOGRAPHY A
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2022
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IF 4.601
Xu Xu,Jiaxin Gao,Di Cao,Xiang Li,Xin Zhao,Shuang Yue,Lei Zhang
In this paper, the novel 3D N-doped magnetic porous carbon spheres (3D N-MPCSs) was constructed via a simple template-free and self-doping method, followed by a direct carbonization of polymer with Fe 3+ for simultaneous activation and magnetization. For the first time, the designed 3D N-MPCSs was used as multifunctional (for extraction and magnetic separation) adsorbents for microwave-assisted derivatization and magnetic solid phase extraction, which exhibited excellent extraction ability for its large surface area, mesoporous structure, spherical morphology, and incorporation of N/O heteroatoms. Moreover, when the microwave-assisted derivatization was combined with the magnetic solid phase extraction, the extraction, derivatization and concentration was completed simultaneously within 135 s. Furthermore, this method has a low detection limit (0.059–0.073 ng mL −1 ) and high enhancement factors (333 < EFs < 382) for trace biogenic amine (BAs). The recoveries were between 92.3 and 108.8% with relative standard deviations less than 5.9%. Overall, a simple, rapid, efficient and sensitive method for the analysis of BAs in food samples was developed, which provided the basis for routine detection of food safety.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
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IF 5.279
Nuo Duan,Mingqian Song,Weiyu Mi,Zhouping Wang,Shijia Wu
It is necessary to detect the biogenic amine (BA) content in food due to their toxicological effects and their role as an index of freshness for protein-rich foods. Aptamer-based techniques have the potential to provide alternative methods for sensitive and efficient monitoring of BAs. Herein, we described the selection and characterization of DNA aptamers for tyramine (TYR) and β-phenethylamine (PHE) using a one-pot coupled with separate selection strategy. During the selection process, melting curve analysis was developed to monitor the enrichment of the aptamer species, and a saturation of the selection was found at the 14th round. Based on the fluorescence assay, aptamers TYR-2 and PHE-2 showed high affinity to TYR and PHE with the dissociation constant values of 64.28 ± 10.4 and 71.64 ± 11.47 nM, respectively. The circular dichromatic and molecular docking technologies were employed for the preliminary binding mechanism analysis. The obtained aptamers TYR-2 and PHE-2 were used in a fluorescence method for the TYR and PHE determination with limits of detection of 0.34 and 0.39 ng/mL, respectively. In addition, the developed aptasensor was further applied to the TYR and PHE detection in pork and beer samples, and the recovery rate was between 95.6 and 104.2%. It was demonstrated that the selected aptamers had enormous potential as a molecular probe for the identification and determination of BAs.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Nuo Duan,Changxin Li,Xiaoyin Zhu,Shuo Qi,Zhouping Wang,Shijia Wu
Histamine (HIS) and tryptamine (TRY) are heterocyclic biogenic amines (BAs) often found in food products. The consumption of products containing high levels of BAs results in a wide range of life-threatening physiological reactions. The work described a Simultaneous coupled with Separate selection strategy for the screening of different aptamers against histamine and tryptamine. Aptamer HIS-3 and TRY-2 exhibited a good binding affinity ; specificity and pH stability to histamine and tryptamine were obtained with a dissociation constant of 11.62 ± 3.241 nM and 6.303 ± 1.758 nM, respectively. The preliminary binding mechanism was analyzed using a circular dichromatic (CD) and molecular docking. Finally, the identified aptamers were used for the development of a colorimetric method for the detection of histamine and tryptamine, achieving limits of detection of 1 ng/mL and 10 ng/mL, respectively. This aptamer-based method was further successfully employed to detect the BAs in fish and pork, providing a potential application for sensitive and efficient detecting multiple food risk factors.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Yanan Liu,Jian Chen,Huan Li,Yanbo Wang
Active packaging has been recognized as an effective approach to extend the shelf life of food, but the rapid release of active substances limits the preservation effect. In this study, gallic acid (GA)-loaded ovalbumin (OVA)/chitosan (CS) nanoparticles with slow-release properties were prepared and embedded into the pectin matrix to refine the rapid release of GA in the pectin and elongate the shelf life of salmon fillets. Our results showed that GA could be released continuously from the OVA/CS nanoparticles. The pectin film incorporated with GA-loaded OVA/CS nanoparticles exhibited good light barrier and mechanical properties. The opacity value of the film reached 1.65 ± 0.06 UA/mm, and the tensile strength and elongation at break were 15.97 ± 1.55 MPa and 7.29 ± 0.42 %, respectively. In addition, the pectin film combined with GA-loaded OVA/CS nanoparticles showed improved antibacterial activity against two common biogenic amine-producing bacteria ( Morganella morganii and Escherichia coli ). Moreover, the nanocomposite film delayed salmon fillets' biogenic amine generation, and the shelf life was extended by 3 days compared with the control group. These promising properties supported using the GA-loaded OVA/CS nanoparticle- pectin films as preservation materials for fish.
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