Food Frontiers
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2024
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IF 7.4
Ziyi Han,Leyu Wang,Panjie Sun,Mengxin Huang,Fei Yu,Junsheng Liu,Yuanyuan Wu,Puming He,Youying Tu,Bo Li
Hyperglycemia and diabetes are common metabolic disorders. It is considered a safe and effective strategy to screen active ingredients from food and herbs for controlling blood sugar levels. Quinic acid (QA) is a natural polyphenolic acid with various health-promoting properties. In this study, QA was found to exhibit a potent inhibitory effect on α-glucosidase activity, with a half maximal inhibitory concentration (IC 50 ) of 5.46 mM. This inhibitory property surpassed that of three common organic acids including gallic acid, malic acid, and citric acid. A combination of 25% acarbose (0.5 mM) and 75% QA (5 mM) (v/v) exhibited synergistic inhibition of α-glucosidase activity. Enzyme kinetics, fluorescence spectra, and molecular docking analyses indicated that QA acted as an uncompetitive inhibitor of α-glucosidase, with hydrogen bonds playing a key role in the intermolecular interactions. Moreover, QA was found to effectively inhibit three major nonenzymatic glycation products including advanced glycosylation end products (AGEs), fructosamine, and α-dicarbonyl in a dose-dependent manner, outperforming the positive control aminoguanidine (AG) within the tested concentration range. Utilizing a Caco-2 cell model, QA demonstrated the ability to inhibit the transmembrane absorption of glucose. This study highlighted QA as a promising food functional factor that had been overlooked in the past, offering potential benefits in improving hyperglycemia, diabetes, and associated complications through the inhibition of α-glucosidase, nonenzymatic glycosylation, and glucose uptake.
研究领域:
食品科学
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分子生物学
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代谢疾病研究
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产品关联
DOI
高影响因子
PROCESS BIOCHEMISTRY
·
2020
·
IF 2.952
Xuelian Zhou,Yi Shen,Yong Xu,Venkatesh Balan
2-Keto- d -gluconic acid (2-KGA) is a high-value-added product from lignocellulosic straw-based biorefining process obtained via cell catalysis using Gluconobacter oxydans ( G. oxydans ); nonetheless, the economical production has some limitations because of the formation of undesirable mixture products of keto-gluconic acid from glucose. In this study, it was demonstrated that pulse addition of hydrogen peroxide (H 2 O 2 ) to synthetic media was beneficial to improve the conversion rate of glucose to 2-KGA by 1.47-fold in G. oxydans . Moreover, some lignocellulosic degradation compounds served as substitute and played the role of H 2 O 2 on corn stover hydrolysate for the production of 2-KGA. By conducting qRT-PCR analysis, eight dehydrogenase genes were identified in G. oxydans , which were responsible for regulating 2-KGA production. Moreover, most of the identified genes were found to produce membrane-bound glucose dehydrogenase and gluconate 2-dehydrogenase, and were up-regulated by adding H 2 O 2 or degradation compounds. These up-regulated genes could be responsible for directing cell catalysis of glucose to 2-KGA in G. oxydans .
研究领域:
生物化学
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微生物学
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产品关联
DOI
高影响因子
ANALYTICAL LETTERS
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2018
·
IF 1.206
Chaohui Chen,Yanan Zhang,Zhengtao Zhang,Rongxiang He,Yong Chen
Here is reported a fluorescent biosensor for glucose detection based on water-soluble and pH-responsive silicon nanodots. The silicon nanodots were prepared using a facile hydrothermal method. The advantages of using the silicon nanodots as glucose sensor are twofold. Firstly, the fluorescence of silicon nanodots was quenched by hydrogen peroxide that was produced from glucose oxidation. Secondly, the fluorescence of silicon nanodots was highly sensitive to gluconic acid that was also produced by glucose oxidation. Our results show that this method detected glucose as low as 0.54 µM with a good selectivity and allowed the determination of glucose in serum samples. This method is also simple, rapid, low-toxic and low-cost, thereby hold high application potential for biological assays.
研究领域:
生物化学
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纳米技术
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分析化学
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产品关联
DOI
高影响因子
PROCESS BIOCHEMISTRY
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2011
·
IF 2.648
Lei Chen,Ning Li,Min-Hua Zong
A glucose-tolerant β-glucosidase was purified to homogeneity from prune ( Prunus domestica ) seeds by successive ammonium sulfate precipitation, hydrophobic interaction chromatography and anion-exchange chromatography. The molecular mass of the enzyme was estimated to be 61 kDa by SDS-PAGE and 54 kDa by gel permeation chromatography. The enzyme has a p I of 5.0 by isoelectric focusing and an optimum activity at pH 5.5 and 55 °C. It is stable at temperatures up to 45 °C and in a broad pH range. Its activity was completely inhibited by 5 mM of Ag + and Hg 2+ . The enzyme hydrolyzed both p -nitrophenyl β- d -glucopyranoside with a K m of 3.09 mM and a V max of 122.1 μmol/min mg and p -nitrophenyl β- d -fucopyranoside with a K m of 1.65 mM and a V max of 217.6 μmol/min mg, while cellobiose was not a substrate. Glucono-δ-lactone and glucose competitively inhibited the enzyme with K i values of 0.033 and 468 mM, respectively.
研究领域:
植物学
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生物化学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
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2016
·Vol. 183
·期 10
·2761-2770
·
IF 4.831
Fang Xin,Wu Xiu-Ming,Hu Xue-Lian,Li Zai-Jun,Wang Guang-Li
We report on a sensitive and selective fluorescent assay utilizing native carbon dots (CDs) as signal transducers. The optical probes 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) or 3,3′-diaminobenzidine (DAB) were employed as substrates of horseradish peroxidase (HRP). It was found that the corresponding oxidation products (ox-ABTS or ox-DAB) quench the fluorescence of CDs, mainly via photoinduced electron transfer in case of ox-ABTS, and via aggregation and inner filter effect in case of ox-DAB. By coupling with bienzyme (glucose oxidase and HRP)-mediated biocatalytic reactions, the method was applied to the determination of hydrogen peroxide and glucose. In case of ABTS as the substrate of HRP, a wide linear range (0.05 to 100 μM) and a very low detection limit (10 nM) for glucose were attained. The method was applied to the determination of glucose in human serum and the results were found to agree well with data provided by a local hospital.
In this work, a sensitive and selective fluorescent assay was developed for probing the enzymatic substrates of hydrogen peroxide and glucose which utilized native carbon dots (CDs) as signal transducer.
研究领域:
生物化学
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分析化学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2016
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IF 4.758
Longjiang Ding,Minggang Zhao,Sisi Fan,Ye Ma,Jingjing Liang,Xingtao Wang,Yawen Song,Shougang Chen
A new Co 3 O 4 electrode material with self-supporting urchin-like hollow microspheres structure was fabricated via a self-assembly method. Incorporating the adsorption and entrapment, the high porosity and large surface area were favorable for the highly effective immobilization of glucose oxidases . Multiple responses, consisting of enzymatic and material catalysis, were subsequently employed and achieved on the microsphere surface. As the radially oriented nanowires array provided a vast number of catalytic active sites, which lowered the energy barrier of glucose redox and acted as a media to boost the electron transfer, the detection potential was largely decreased. Furthermore, numerous electron transport paths in a short distance were formed among the interconnected bridge-like nanowires, resulting in improved electron transfer efficiency. The prepared glucose biosensor exhibited extra high sensitivity (102.77 μA mM −1 cm −2 ), fast response (3 s) and outstanding selectivity and stability at a lower applied potential (0.35 V).
研究领域:
生物传感技术
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材料科学
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电化学
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产品关联
DOI
高影响因子
Chemistry-An Asian Journal
·
2022
·Vol. 17
·期 18
·e202200556
·
IF 4.839
Yue Zheng,Di Xu,Lei Zhang,Xi Chen
Graphical Highly-valued glucosaminic acid was produced by the oxidation of chitin derived glucosamine under ambient air and base-free conditions. In this study, glucosaminic acid (GlcNA) was produced with the highest yield of ∼85% from chitin biomass-derived glucosamine (GlcN) at 35 °C under atmospheric air in water, by using zinc oxide-supported gold nanoparticles (Au/ZnO). The Au/ZnO catalyst prepared by the deposition-precipitation (DP) method displayed remarkably superior catalytic performance to that prepared by the deposition-reduction (DR) method, which led to >2-fold enhancement in product yield. A lower apparent activation energy (E a ) was observed over the Au/ZnO (DP) for GlcNA formation compared with the Au/ZnO (DR) catalyst. Based on control tests and structural characterizations, the average particle size of Au nanoparticles (NPs) and the surface oxygen vacancy (O V ) sites were the crucial factors on the catalytic activity. The work puts forward a green, mild and efficient approach to synthesize valuable amino acid from ocean-based chitin biomass resources with air as the oxidant, adding on new possibilities of chitin biorefinery.
研究领域:
生物化学
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催化化学
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绿色化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Ya Gao,Yu Huang,Junliang Ge,Lei Sun,Anwei Zhou,Shujun Feng,Yurui Xu,Xinghai Ning
An accurate measurement of sweat glucose is a promising alternative to invasive finger prick blood test, and may provide effective self-monitoring of blood glucose with good patient compliance. Herein, an interrelated catalytic enzyme system has been developed, termed as CataFlower, which is composed of nanoflower MoS 2 (peroxidase) decorated with GOx (glucose oxidase) and MnO 2 (oxygen generator), and exhibits synergistic oxidative capability for sensitively monitoring sweat glucose. CataFlower can not only generate oxygen in situ to maximize GOx activity, but promote peroxidase-triggered H 2 O 2 oxidation of methylene blue , resulting in sensitive colorimetric detection of glucose. We identify that CataFlower can precisely detect glucose with a detection limit of 10 μM, allowing for measuring glucose levels in different biological samples, such as blood and urine. Particularly, CataFlower is capable of monitoring dynamic changes in sweat glucose with high sensitivity and accuracy during exercise. Therefore, CataFlower provides a stepping stone to eliminate invasive blood tests, significantly improving the diagnosis and management of diabetes mellitus.
研究领域:
生物化学
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医学诊断
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材料科学
·
酶工程
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2018
·
IF 3.129
Xueyan Ren,Peipei An,Xin Zhai,Shuo Wang,Qingjun Kong
Antibiotic overuse has caused concerns owing to the emergence of antibiotic resistant microorganisms and the accompanying decreased bacteria-inhibiting effectiveness of proffered treatments. Pterostilbene, derived from XinJiang wine grape, is a natural polyphenol, and has attracted considerable attention for its promising antifungal effects. However, its inhibitory activities and mechanisms of action against bacteria are still unknown. In this study, pterostilbene inhibited the growth of Gram-positive Staphyloccocus aureus and Gram-negative Escherichia coli effectively. Electron microscope observations showed that the morphological structures of S. aureus and E. coli were significantly damaged by pterostilbene. Cells became irregular, shriveled, and lysed, while nucleic acids and proteins leaked, and DNA content decreased. Furthermore, confocal laser scanning microscopy experiments revealed that bacteria treated with pterostilbene produced reactive oxygen species, and the cytomembrane potential increased. To further clarify the molecular mechanism, changes in the expression levels of four genes in two bacteria were confirmed by qRT-PCR. Pterostilbene cause the up-regulation of DinF (an oxidative stress gene). An associated down-regulation of PaL, MtgA, and NagA (genes involved in cell wall synthesis) is also attributed to pterostilbene treatment. This is the first time that the mechanism of pterostilbene against S. aureus and E. coli is presented in literature.
研究领域:
微生物学
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药理学
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分子生物学
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2022
·Vol. 41
·期 1
·57-63
·
IF 5.56
Ziwei Su,Xiangxiang Zhang,Weichen Wang,Mingdong Dong,Xiaojun Han
Comprehensive Summary The bottom-up construction of self-powered artificial cells is significant to understand the energy supply and metabolism of nature cells. Here, we demonstrate an efficient manner to build thylakoid-containing artificial cells, which continuously convert light energy into chemical energy to supply adenosine 5’-triphosphate (ATP) under light illumination. The production of ATP supplies energy to promote the biological enzyme cascade reactions, where glucose is transformed into glucose 6-phosphate (G6P) under the catalysis of hexokinase (HK). G6P was further converted to gluconolactone 6-phosphate (PG) in the presence of 6-phosphate dehydrogenase (G6PDH), meanwhile NADP + was converted to nicotinamide adenine dinucleotide phosphate (NADPH). The self-powered artificial cells were demonstrated to generate ATP and NADPH successively, which provided a way for building more complicated artificial cells.
研究领域:
生物化学
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细胞生物学
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合成生物学
·
能量代谢
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产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Gongwei Tian,Jianhui Chen,Dan Yang,Cuiyuan Liang,Qinyi Zhao,Yan Liu,Weihong Lu,Dianpeng Qi
Wearable sensing devices provide a promising approach for non-invasive health detection. However, most flexible devices are inevitably susceptible to wear and mechanical degradation in practical applications due to stretching, cutting, or overuse, resulting in device malfunction. Therefore, it is necessary to develop sensing devices with self-healing and stretchable capabilities. Here, an in-situ self-healing and stretchable sweat glucose sensor is fabricated by depositing Au on the synthetic self-healable PDMS 0.9 -IPDI elastomer and modifying polypyrrole/glucose oxidase on the sensing position, respectively. This sensor can recover electromechanical properties through dynamic hydrogen bonds after fracture and self-healing 4 h at room temperature, and maintain stable sensing properties under 50% strain, which satisfies the strain required by human skin in daily activities. In addition, the modified polypyrrole film on the Au electrode increases the electrochemically active area of the working electrode by 3-4 times and the sensor exhibits a sensitivity of 62.60 μA·mM -1 ·cm -2 in the linear range of 0-1 mM, with the detection limit is as low as 12.58 μM. Furthermore, the sensor can accurately and reliably detect the glucose content in human sweat samples, providing a novel approach for the practical application of glucose sensors.
研究领域:
生物医学工程
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材料科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Polymers
·
2019
·Vol. 11
·期 3
·551
·
IF 3.164
Zhiping Jiang,Yonggang Shangguan,Qiang Zheng
A polyelectrolyte film-coated electrode for the quantitative detection of glucose was reported. Carbon nanotubes, graphene oxide and polyelectrolyte with a ferrocenyl group were used to modify an enzyme electrode to facilitate the electron transfer between glucose oxidase and the electrode. Cyclic voltammetry and amperometric methods were adopted to investigate the effects of different polyelectrolytes and carbon nanomaterials on the electrochemical properties of enzyme electrodes. The results indicate that the ferrocenyl groups on a polyelectrolyte skeleton act as a mediator between the redox center of glucose oxidase and the electrode, which efficiently enhances the electron transfer between a glassy carbon electrode and glucose oxidase. The calibration curve of the sensor shows a linear range from 0.2 to 5 mM for glucose response. The sensor can achieve 95% of the steady-state current within 10 s. The electrodes also present high operational stability and long-term storage stability.Keywords:ferrocene;biosensor;redox polymer;polyelectrolytes
研究领域:
生物传感器
·
电化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）