查找使用阿拉丁产品的科研文献

Flavor enhancement of corn oil through the solid-state complex-enzyme hydrolysis: Process optimization and mechanistic insights

Guohao Sun,Shangde Sun,Yulan Liu,Hao Zhang,Yuxiang Ma,Yaoyao Zhang,Heng Du,Talha Ashfaq,Xuze Zhang,Zisong Li,Aiyue Wang

The monotonous flavor of corn oil has limited its development in high-end markets. Although desirable flavor can be obtained by high-temperature processing, nutrient loss and safety risks hinder its application in fragrant corn oil (FCO) production. To address these limitations, a solid-state complex-enzyme hydrolysis (CEH) technology was developed to enhance FCO flavor through targeted precursor enrichment under mild conditions. The alcalase-glucoamylase system was optimized by multi-objective optimization under 15% water addition, generating free amino acids and total sugars at 538.31 mg/100 g and 2945.27 mg/100 g. Compared to untreated samples, more nutritional components were preserved in FCO by CEH. Simultaneously, volatile organic compounds were enhanced by 84.4%, particularly Strecker aldehydes and heterocyclic compounds. Synergistic mechanisms were elucidated through molecular docking, revealing selective substrate degradation and reduced competitive inhibition in enzymatic hydrolysis. Multi-pathway flavor formation involving Maillard reaction, lipid oxidation, and physical release was established, providing theoretical foundation for high-value FCO development.

Fabrication of low-fat mayonnaise via egg white protein fibril-stabilized emulsion gels: Enhanced interfacial adsorption and network support

Wanyi Dong,Mohamed Salama,Kazuhiro Isobe,Akihiro Handa,Xi Huang,Zhaoxia Cai

Functional characterization of a canonical C-type lectin from Mytilus coruscus: Roles in innate immunity and feeding processes

Ying-rong Jin,Chuan-yue Chen,Ze-kai Wang,Pan Qin,Zhi Liao,Xiao-lin Zhang,Xiao-jun Yan

C-type lectins (CTLs) play crucial roles in immune defense and feeding regulation, serving as key mediators of pathogen recognition and nutrient uptake in invertebrates. Although previous studies have demonstrated the involvement of CTLs in the immune response of Mytilus coruscus , their role in feeding regulation remains unclear. In this study, we identified a C-type lectin, designated MytiCTL17A , from M. coruscus and investigated its dual function in immune defense and feeding behavior. The purified recombinant MytiCTL17A binds to LPS and PGN. Additionally, it binds to both Gram-positive and Gram-negative bacteria, promoting bacterial agglutination. Notably, this agglutination can be inhibited by PAMPs. These findings confirm that rMytiCTL17A functions as a pattern recognition receptor (PRR), recognizing PAMPs, binding to bacteria, and promoting their agglutination, thereby playing a role in immune defense. Additionally, rMytiCTL17A also exhibits direct antibacterial activity. In feeding experiments, rMytiCTL17A can bind to microalgae and reduce the feeding rate of M. coruscus , indicating its involvement in the feeding process. Moreover, rMytiCTL17A can recognize carbohydrates (D-Man, D-Gal, Glu, Mal, and Suc) on the surface of microalgae and induce microalgal agglutination. Notably, this agglutination can be inhibited by D-Man, demonstrating its dependence on carbohydrate recognition. Thus, rMytiCTL17A also participates in feeding by recognizing microalgae through specific carbohydrates. This study highlights the multifunctionality of rMytiCTL17A and provides new insights into the roles of C-type lectins in bivalves, with potential implications for aquaculture in terms of enhancing disease resistance and feeding efficiency.

Natural sugar substitute Rebaudioside M: Efficient and cost-effective biosynthesis via host engineering, cascade biocatalysis, and substrate feeding

Xiang Luo,Yang Wang,Man Zhou,Qiaolan Sun,Xue Wang,Xiaojie Yu,Li Chen,Hua Yang,Cunshan Zhou

Rebaudioside M is a desirable zero-calorie sweetener, but its low natural abundance limits industrial application. Here, enzymatic conversion of Rebaudioside D was intensified through host engineering, cascade biocatalysis, and substrate feeding. Expression of the glycosyltransferase in a eukaryotic host increased the soluble enzyme fraction from 18% to 92% and improved specific activity by 1.46-fold compared with the bacterial system. Coupling with sucrose synthase enabled in situ regeneration of the sugar donor from inexpensive sucrose. Kinetic analysis identified substrate depletion as the dominant bottleneck in batch reactions. A fed-batch feeding strategy increased the product titer to 28.38 g L −1 with 91.6% conversion, representing a 3.99-fold improvement over batch mode. This integrated strategy provides a practical basis for cost-efficient production of next-generation natural sweeteners.

Intraphase electronic coupling in Ni3Sn2S2@nitrogen-doped carbon for high-rate and durable potassium-ion batteries

Yonghuan Fu,Yulian Dong,Jingyao Huo,Miaomiao Pang,Huaping Zhao,Jinhui Hao,Yong Lei

Bimetallic sulfide, with its high theoretical capacity, is one of the preferred anodes for high-energy-density potassium ion batteries (PIBs). However, its low conductivity, high volume expansion, and slow electrode reaction kinetics have limited its practical application. This study developed a freeze-drying-calcination coupled strategy to fabricate a porous nitrogen-doped carbon matrix embedded with Ni 3 Sn 2 S 2 bimetallic sulfide nanoparticles (Ni 3 Sn 2 S 2 @NC). The enhanced electrochemical kinetics are primarily attributed to the strong electronic interactions between Ni and Sn atoms within the hexagonal crystalline framework. The reduced adsorption energy of potassium ions (K + ) and the significantly enhanced diffusion coefficient (∼2.88 × 10 −10  cm 2 /s) are attributed to the electronic coupling effect within the bimetallic sulfide phase. The electronic interaction effectively promotes both K + transport and electron transfer during charge/discharge cycles, thereby delivering the Ni 3 Sn 2 S 2 @NC composite with good specific capacity (∼667.7 mAh/g at 0.1 A/g) and ultralong cycling stability at high rates (∼170.6 mAh/g under 2.0 A/g after 2000 cycles). When assembled with perylene-3,4,9,10-tetracarboxylic dianhydride (PTCDA) as the cathode in PTCDA||KFSI-DME||Ni 3 Sn 2 S 2 @NC, the device achieves a high energy density of ∼226 Wh/kg with stable cycling performance. This work introduces a valuable perspective for the kinetic engineering of high-rate electrodes through intrinsic electronic structure modulation.

Sugar profile in monofloral honeys from China: Implications for quality control

Ru Jia,Sha Yan,Xuezhe Huang,Zongyan Cui,Xiaofeng Xue

Honey is primarily composed of sugars, with their profiles governing its quality. Twelve types of sugars in 784 honey samples, including 20 types of Chinese monofloral honeys, were accurately quantified by ultra-high performance liquid chromatography coupled with evaporative light scattering detection. Turanose (1.48 ± 0.63 g/100 g) was the most abundant oligosaccharide, followed by maltose (1.33 ± 0.43 g/100 g), and erlose (0.96 ± 0.80 g/100 g) was the predominant trisaccharide. Using unsupervised clustering, these honey samples were segmented into three clusters: high-oligosaccharide (e.g., jujube and fennel honey), high-glucose and low-oligosaccharide (e.g., rape, lychee and rubber honeys) and moderate-oligosaccharide clusters. The correlation analysis revealed strong correlations among turanose, maltulose, kojibiose and isomaltose, suggesting co-transformation among these sugars during honey ripening process and guiding future research directions. This study elucidated the sugar profile characteristics of Chinese honey, laying the foundation for enhancing its quality.

The 'two birds with one stone' strategy of flower-like multi-shell hollow microsphere/boron nitride composite achieves dual functionalities of efficient microwave absorption and excellent thermal management

Renquan Wu,Yuxin Tian,Xuzhou Jiang,Hongying Yu,Yijing Sun

Electronic devices not only face electromagnetic interference issues but also must address challenges such as performance degradation and reduced reliability caused by heat accumulation. Therefore, the development of dual-functional microwave absorbing materials (MAMs) that possess both efficient microwave absorption and excellent thermal management performance has become a crucial and urgent material science issue to be addressed. This study successfully fabricated flower-like multi-shell hollow microsphere/boron nitride (FMSHS/BN) composites through a combined strategy of surface modification, electrostatic self-assembly, and sequential templating. The SEM and TEM images of the FMSHS/BN-6 (the BN content is 60 mg) reveal that FMSHS is uniformly distributed between the BN layers, effectively mitigating the issue of local impedance mismatch caused by agglomeration. As a result, the FMSHS/BN-6 exhibited exceptional microwave absorption performance, achieving a minimum reflection loss (RLmin) of -61.73 dB and a maximum effective absorption bandwidth (EABmax) of 5.60 GHz. Meanwhile, the thermal conductivity of the composite reached 3.87 W/(m·K), representing a 921% improvement compared to that of pure FMSHS (0.42 W/(m·K)). These results demonstrate that the FMSHS/BN-6 realizes the synergistic integration of efficient thermal management and outstanding microwave absorption via a "two birds with one stone" design strategy, showing great potential for addressing thermal accumulation and high-frequency electromagnetic interference in electronic devices.

Fine-Core Highly Water-Retentive Hydrogel Flexible Optical Fiber for Long-Term Stable Sensing Application

Chunbiao Liu,Zhihai Liu,Yu Zhang,Yifan Qin,Mengyao Zhang,Wei Jin,Wenxuan Gu,Chen Shi,Libo Yuan

Hydrogel flexible optical fibers (HFOFs) have great potential in environmental monitoring and biomedical applications. However, current HFOFs face significant challenges, including the presence of large amounts of free water and low fiber crosslinking density, which result in poor water retention and environmental tolerance. These issues hinder the stable use of HFOFs as sensing waveguides. This study proposes for the first time a high-water-retention, fine-core HFOF. HFOFs are spun from a polyacrylamide hydrogel material containing glycerol using the drawing spinning method. During the drawing and stretching process, the fiber network porosity decreases, and self-assembly induced by water evaporation generates numerous hydrogen bonds. The hydroxyl groups in the glycerol molecules form hydrogen bonds with water molecules, while a large amount of bound water is generated, thereby enhancing the water retention performance of the HFOFs. HFOF demonstrates excellent water retention (water loss rate: <0.1%/day), stretching performance (500%), light-guiding ability (1.45 dB/cm), and recovery properties (1 min). The highly water-retentive HFOFs are applied in various scenarios, including bionic sensors for vibration detection and flexible sensors for monitoring human respiration and heartbeat. This work offers a new solution for improving the stable use of HFOFs and provides new inspiration for bionic monitoring and wearable human sensors.

A molecularly imprinted electrochemical sensor based on dual functional monomers for selective determination of nimodipine

Ying Wang,Xuyuan Sun,Minmin Liu,Zhengyuan Dai,Xiaoyu Yang,Li Li,Yaping Ding

As a core medication for the prevention and treatment of cerebral vasospasm, especially after aneurysmal subarachnoid hemorrhage, real-time monitoring of nimodipine (NMD) concentration helps evaluate the adequacy of drug therapy and holds great significance for ensuring patient life and health. Herein, based on dual functional monomers, a molecularly imprinted electrochemical sensor (MIECS) for the detection of NMD with nitrogen-doped multi-walled carbon nanotubes (N-CNTs) and Fe-MOFs was developed. Fe-MOFs provided a large specific surface area, offering more space for generating imprinting sites, while N-CNTs enhanced the conductivity of the electrode. 2-Amino-5-mercapto-1,3,4-thiadiazole (AMT) and o-phenylenediamine (o-PD) served as dual functional monomers and NMD as the template molecule. A molecularly imprinted polymer (MIP) membrane was prepared on the electrode surface by electropolymerization. Compared to single functional monomers, the dual functional monomers exhibited better selectivity and specificity in NMD recognition. Under optimal experimental conditions, the response of the MIECS to NMD showed a linear relationship ranging from 10−14 M to 10−8 M, with a detection limit of 2.97 × 10−15 M. Satisfactory recovery rates were obtained in the detection of human serum and tablets. This multi-parametric enhancement establishes a new paradigm for therapeutic drug monitoring in clinical neurology and pharmaceutical quality control.

Monomer Concentration Regulation Enables Highly Cross-Linked Polyamide Membranes for Robust High-Temperature Separation

Wan-Ting Lin,Chang Liu,Xiao-Wei Luo,Wan-Long Li,Zi-Lu Zhang,Si-Yuan Zhang,Zhi-Kang Xu,Ling-Shu Wan

Separation under high temperature is a crucial challenge for polymer nanofiltration (NF) membranes. Polyamide NF membranes with favorable compactness and pore size distribution are promising for boosting their thermal stability and high-temperature separation performance. Despite recent strategies to improve thermal stability, the effect of polyamide membrane compactness on structural changes and separation performance at high temperatures remains insufficiently explored. Herein, we propose a straightforward and effective concentration regulation strategy to address this challenge in the classic piperazine-trimesoyl chloride (PIP-TMC) interfacial polymerization system, focusing on the precise tuning of the polyamide network structure and the high-temperature NF performance through the controlled adjustment of monomer concentration and the relative ratios. Polyamide layer fabricated with a PIP/TMC ratio of 30:1.5 exhibits higher cross-linking degree than that with a PIP/TMC ratio of 2:1.5, and the NF membrane exhibits exceptional thermal stability, with MgSO4 rejection remaining >98.5% at 85 °C. Molecular dynamics simulations reveal that denser polyamide network possesses significantly weaker segmental motion, along with smaller changes in pore size distribution and fractional free volume at elevated temperatures. This concentration regulation strategy effectively enhances the intrinsic thermal stability without complex modifications and provides critical insights into polyamide segment evolution at high temperature.

Uniform Tellurium Quantum Dots for Mode-Locked Fiber Lasers with Low Starting Threshold

Yun Ye,Minqing Chen,Qianlin Wang,Yuhao Huang,Xvjing Xv,Ziwen Chen,Chenlong Zhou,Hui Long,Yu Zhao,Lili Tao

Ultrafast lasers have extensive and important applications in precision processing, biomedicine, ultrafast spectroscopy research, and communication fields. Saturable absorber materials are key components in obtaining ultrafast lasers. However, at high mode-locked pump power, the saturable absorber is easily damaged, greatly reducing its operational stability and service life. Here, we report the synthesis of uniform tellurium quantum dots with an average size of ∼3 nm and stable traditional soliton mode-locked fiber laser achieved at an extremely low pump power of 30 mW, with an output pulse width of 700 fs based on the tellurium quantum dot saturable absorber. As the pump power further increased, the output pulse width was compressed to 578 fs at 150 mW. Even more interestingly, by adjusting the pump power, the phenomenon of bound soliton mode-locked was also discovered. The experimental results demonstrate that the synthesized uniform tellurium quantum dots exhibit an ultralow mode-locked threshold, enabling ultrafast laser operation at low pump powers. This material represents a promising saturable absorber with unique application potential.

Ultrasound-assisted combined with natural deep eutectic solvents for complanatoside A extraction: Optimization, identification, and bioactivity evaluation

Penghua Shu,Wenhao Zou,Yu Meng,Jiaqi He,Tingting Guo,Xinfeng Fan,Di Hu,Hao Yin,Shuxian Fan,Na Wang,Simin Liu,Lu Lv,Si Chen,Xialan Wei,Jihong Huang

Astragali complanati Semen (ACS) is frequently used as Chinese medicines and functional foods. Complanatoside A (CA), a key bioactive phytochemical isolated from ACS, exhibits broad-spectrum pharmacological properties. This investigation systematically formulated sixteen natural deep eutectic solvent (NADES) systems by combining three hydrogen-bond acceptors with eleven hydrogen-bond donors at varying molar ratios. These NADES formulations were subsequently employed in tandem with ultrasonic-assisted extraction to isolate CA. Optimization studies identified the following parameters as most effective for extraction using the L -proline/levulinic acid-based NADES (molar ratio 1:2): 60℃, 30 min, 40 % water content, liquid-solid ratio 25 mL/g, achieving an extraction yield of 1.57 %. The adsorption and desorption conditions were optimized employing a macroporous resin for the recovery of CA, while the reusability of the NADES solvent was thoroughly evaluated throughout the extraction process. The CA extract exhibited significant tyrosinase and α-glucosidase inhibitory activity. These findings establish ultrasound-assisted NADES extraction as a sustainable methodology for efficient extraction of CA from ACS, providing valuable insights for eco-friendly extraction and pharmacological exploitation of plant-derived bioactives.