MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Xin Zhang,Li Tian,Kexin Wu,Zhuo Sun,Qian Wu,Xiangyu Shan,Yingjie Zhao,Ruizhan Chen,Juan Lu
An electrochemical luminescence (ECL) sensor for the detection of glucose was constructed by covering CdTe nanomaterials, ZIF-7 and glucose oxidase (GOD) on the electrode surface, respectively. CdTe and ZIF-7, as co-reaction promoters, could amplify ECL signals through synergy effect. The results demonstrated that ECL intensity increased when the concentration of glucose increased. Based on the change of ECL intensity, a method for the determination of glucose was established. Possible response mechanisms were also studied. The parameters of measurement conditions were optimized. The linear range of glucose was 2.0 × 10 −10 ∼ 2.0 × 10 −5 mol L −1 and the detection limit was 6.7 × 10 −11 mol L −1 under the optimal conditions. The constructed sensor had high sensitivity and selectivity. The results were satisfactory for the determination of glucose in real human serum samples.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Mei Zhang,Qianqian Kong,Jingcheng Huang,Yaodong Xiang,Guanjie Wang,Jie Han,Yemin Guo,Shancang Zhao,Xia Sun
Within this work, an electrochemiluminescence (ECL) aptasensor was constructed for the sensitive detection of diazinon . Primarily, AuNPs not only provided a platform for luminol fixation due to their favorable bioactivity and conductivity , but they facilitated the improvement of ECL signals. Furthermore, three-dimensional (3D) flower-like ZnONPs were obtained by hydrothermal synthesis , which could be an efficacious catalyst for the luminol-hydrogen peroxide (luminol-H 2 O 2 ) system, whereby the ECL signal of the sensor could be amplified. Given the large specific surface area of 3D flower-like ZnONPs, modification of positive charges on their surface could well immobilized the electronegative aptamer chains on the electrode surface , and when diazinon (DZN) was added, the aptamer combined with DZN to form a complex to hinder the ECL intensity, and the detection of DZN was achieved by the change of ECL signal intensity. After evaluated the experimental parameters, the detection limit of DZN was 0.0109 nM (S/N = 3), and the linear range was 0.0328–3.28 × 10 4 nM. The proposed ECL aptasensor had comparatively satisfactory detection limits, which provided a reasonable reference for other contaminant residues detection.
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PHYSICS OF FLUIDS
·
2022
·Vol. 34
·期 3
·
IF 4.98
Yuan Shuai,Zhou Mingyong,Peng Tao,Li Qiang,Jiang Fengze
Achieving rapid mixing of different liquids in a short distance is important in various biochemical applications. Herein, a novel planar mixer with staggered Z-shaped baffles is proposed. Numerical investigations are carried out to evaluate its mixing ability based on mixing quality and pressure drop when the Reynolds number (Re) varies from 0.1 to 50. The Lyapunov exponent, the Poincaré map, and the vortex visualization are conducted to comprehensively analyze the chaotic state and the mixing mechanism. Results show that the proposed mixer exceeds 0.9 mixing efficiency when 0.1 ≤ Re and Re ≥ 8. As Re ≥ 8, different vortex patterns appear by changing the inlet configuration. The disturbance for fluids induced by a vortex on the mass transfer surface is not only dependent on its intensity but also related to the position of the vortex/vortex leg. The proposed planar mixer, inducing a single vortex or vortex pair with different directions, presents different mixing performance when the Re varies from 8 to 50, from which the approach of the rotating vortex that can mainly improve the mixing quality is found. This well explains the chaotic mixing behavior observed in the planar mixer with Z-shaped baffles, which to date has not been studied before. Furthermore, the micromixer is fabricated, tested, and applied for luminol–peroxide chemiluminescence detection to characterize its performance.
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INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER
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2022
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IF 5.431
Shuai Yuan,Mingyong Zhou,Xijiang Liu,Qiang Li,Bingyan Jiang
Microfluidic-based enrichment techniques have had a profound impact on chemistry, owing to their ability to improve the sensitivity of detection by increasing the concentration of analytes in minute fluid volumes. Herein, concentration enrichment of analyte solutes is achieved by using voltage assist in a microchip with two reservoirs. A numerical model is developed to describe the complex analyte focusing process, which to date has not been investigated before. Numerical computations are systematically conducted to study the influences of applied voltage, mass transfer coefficient, electrode location, reservoir diameter and number of mass transfer channels on the concentration enrichment in a designed Polydimethylsiloxane (PDMS)/Glass microfluidic electrophoresis chip. The electric fields, concentration distributions, and electrophoretic fluxes are obtained, which can reveal insightful enrichment mechanisms. It is found that the factors that can increase the electrophoretic flux in the mass transfer channel and enhance the electric field strength in the enrichment reservoir, such as moving the electrode back or increasing the number of mass transfer channels, are particularly important to improve the enrichment performance. The final microchip after considering the Joule heating effect has a 335-fold and 2.8-fold maximum and average concentration enrichments, respectively. Furthermore, this method is applied to the experimental enrichment of Rhodamine B and heavy metal cadmium (II) ions to test it performance.
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RSC Advances
·
2022
·Vol. 12
·期 30
·19246-19252
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IF 4.036
Qiaoli Zhang,Yang Yang,Junmei Xia,Yingying Zhang,Shilei Liu,Zhiqin Yuan
It has been a challenge to achieve rapid, simple, and effective discrimination of organophosphorus nerve agents (typical chemical warfare agents) due to the similar chemical properties of the targets such as sarin, soman, cyclosarin and VX. In this study, we propose a chemiluminescence sensor array that can effectively discriminate organophosphorus nerve agents by organophosphorus-H2O2 reaction, which produces peroxyphosphonate intermediate and regulates the chemiluminescence intensity. A simple chemiluminescence sensor array based on different chemiluminescence characteristics of the four organophosphorus nerve agents in the luminol–H2O2 system and layered double hydroxide–luminol–H2O2 system has been constructed. Four agents can be well distinguished at a concentration of 1.0 mg L−1 when linear discriminant analyses and hierarchical cluster analyses are smartly combined. The high accuracy (100%) evaluation of 20 blind samples demonstrates the practicability of this proposed chemiluminescence sensor array.
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PubMed
高影响因子
MICROCHIMICA ACTA
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2022
·Vol. 189
·期 9
·1-8
·
IF 6.408
Li Shuhuai,Luo Jinmei,Wu Yuwei,Ma Xionghui,Pang Chaohai,Wang Mingyue,Luo Jinhui,Zhang Chenghui,Tan Gaohao
Considering the limitations associated with existing methods for the detection of trace amounts of trichlorfon, this paper proposes a novel molecularly imprinted electrochemiluminescence (ECL) sensor for the detection of trichlorfon by utilizing the double enhancement effect of trichlorfon and Ag nanoparticles supported by multi-walled carbon nanotubes (MWCNTs/Ag NPs) in a luminol–H 2 O 2 ECL system. Here, trichlorfon was electropolymerized on the surface of the MWCNT/Ag NP–modified gold nanoelectrode with o -phenylenediamine to prepare the molecularly imprinted polymer-based sensor. After eluting the trichlorfon, imprinted holes for the identification of trichlorfon were retained on the sensor, which were used as signal switches to obtain different ECL intensities through the adsorption of different concentrations of trichlorfon. The ECL signal of the sensitized luminol–H 2 O 2 was doubly enhanced by the MWCNTs/Ag and trichlorfon, improving the sensitivity of the sensor. The trichlorfon concentration was positively correlated with the enhanced ECL intensity of the sensor in the range 5.0 × 10 −8 –5.0 × 10 −11 mol L −1 , and the detection limit of trichlorfon was 3.9 × 10 −12 mol L −1 . Moreover, the proposed sensor was successfully applied to the detection of trichlorfon residues in real samples, and the recovery ranged between 91.8 and 109%. Graphical abstract A molecularly imprinted electrochemiluminescence sensor for trichlorfon detection by utilizing the double enhancement effect of trichlorfon and Ag nanoparticles supported by multi-walled carbon nanotubes in a luminol–H 2 O 2 ECL system. The dual enhancement of the ECL signal improved the sensitivity of the sensor.
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MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Jiang Chang,Deying Zou,Honglin Ren,Xilin Liu,Meng Li,Zhaozhao Si,Cheng Han,Zengshan Liu,Shiying Lu,Pan Hu
The 10-kDa chemokine interferon-gamma-inducible protein 10 (IP-10) is considered one of the most promising biomarkers for diagnosing both tuberculosis and COVID-19 infections. The blood samples of patients at different disease states contain different levels of IP-10, which need to be detected in a rapid, specific and ultrasensitive manner. Here, we report a bienzymatic chemiluminescence sandwich immunoassay (BCSI) assay for the ultrasensitive and stable detection of IP-10. In this assay, IP-10 is first efficiently captured using a double-antibody sandwich strategy. The detection antibody is linked to catalase (CAT) via a streptavidin–biotin signal amplification system to achieve highly efficient conversion of hydrogen peroxide (H 2 O 2 ) to oxygen and water. In the chemiluminescence (CL) reaction, horseradish peroxidase (HRP) acts as an efficient catalyst, and 4-bromophenol acts as an enhancer for the cyclic transition of HRP, which results in a strong and durable CL signal. The bienzymatic catalysis with CAT and HRP and the potentiation of 4-bromophenol enables the assay to be ultrasensitive and stable. The CL intensity was found to be well correlated with the detection of IP-10 at levels in the range of 0.71 to 125,000 pg/mL, which covers more than 6 orders of magnitude, with a detection limit of 0.63 pg/mL. The coefficient of variation was 1.49%, and the recovery range of IP-10 in serum was 86.21%-104.57%. This assay provides a wide linear range and high sensitivity and may be a promising method for the high-throughput detection of IP-10 in the diagnosis of tuberculosis and COVID-19.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Han Zhou,Yi Cai,Min Zhang,Wei Li,Yong Zhao
A miniature luminol chemiluminescence system based on atmosphere microplasma is proposed for detection without any catalysts. In our research, atmosphere microplasma jet is employed to oxidize luminol and produce chemiluminescence instead of H 2 O 2 . The transport of OH radicals to the plasma-liquid interface and induce the chemiluminescence. The weight of the system is only 3.6 kg (including a 1.2 kg laptop), and the power consumption of the microplasma is only 0.045 W. The mechanism of luminol chemiluminiscence induced by microplasma jet and generation of microplasma jet are investigated in this study. A 1 mL sample solution is sufficient for trace 3-NPA determination within an analysis time of 6 min. In the range of 0.03–10 mg L −1 , 3-NPA can be quantitatively analyzed along with a detection limit of 0.008 mg L −1 . In addition, the proposed system is employed for real-world samples detection, including water samples, brown sugar and tainted sugarcane, which demonstrates the reliability and practical feasibility of the detection method.
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ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Jing Lei,Wei Liu,Yan Jin,Baoxin Li
Defect engineering is an effective strategy to improve the catalytic activity of metal oxides, and quantitative characterization of surface defects is thus vital to the understanding and application of metal oxide catalysts. Herein, we found that ZnO nanoparticles with oxygen vacancy could trigger the luminol–H2O2 system to emit a strong chemiluminescence (CL), and the CL intensity was strongly dependent on the oxygen vacancy of the ZnO nanoparticles. The mechanism of this CL reaction was discussed by means of the electron-spin resonance spectrum, X-ray photoelectron spectrum (XPS), and CL spectrum. The oxygen vacancy-dependent CL was attributed to the ability of the oxygen vacancy to readily adsorb and further dissociate H2O2 into active •OH radicals. Taking advantage of this oxygen vacancy-dependent CL, we presented one method for quantifying the oxygen defects in ZnO. Compared with the current evaluation techniques (XPS and Raman spectroscopy), this CL method is rapid, low-cost, and easy to operate. This work introduces the CL technique into the field of material structure–property evaluation, and provides a new approach for exploring the defect function in ZnO defect engineering.
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ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Fuad Abduro Bushira,Shimeles Addisu Kitte,Yong Wang,Haijuan Li,Ping Wang,Yongdong Jin
In this study, an effective oxygen vacancy (Ov)-involved luminol-dissolved oxygen (O2) electrochemiluminescence (luminol-DO ECL) system was developed and exploited for ECL sensing applications through significant plasmon enhancement of the Ov-involved weak luminol-DO ECL signals by the combined use of Cu-doped TiO2 oxygen vacancy and a Au@SiO2 nanomembrane. The results disclosed that the ECL response of the corresponding system could be synergistically boosted, and the plausible underlying mechanism has been discussed. Furthermore, for the first time, the developed system has been successfully applied for the highly sensitive detection of alkaline phosphatase with a low limit of detection of 0.005 U/L, with an excellent linear range from 0.005 to 10 U/L, as well as good stability and reproducibility.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xiaojie Shi,Huimin Liu,Mei Zhang,Fengzhen Yang,Jiansen Li,Yemin Guo,Xia Sun
An ultrasensitive aptasensor based on luminol-hydrogen peroxide (H 2 O 2 ) electrochemiluminescence (ECL) system was proposed for determination of profenofos. AuNPs decorated with MWCNTs ( [email protected] ) were used to provide a favorable microenvironment for luminol loading and improve the ECL signal due to their good biological activity, excellent electrical conductivity and high specific surface area. Gold and silver core-shell nanoparticles ( [email protected] ) could act as effective catalyzer to accelerate H 2 O 2 decomposition to produce various intermediate reactive oxygen species for signal amplification and thus increased the ECL signal again. Under optimal conditions, the proposed ECL aptasensor obtained an extremely low detection limit of 5.32 fg/mL (S/N = 3) with a good detection range from 100 fg/mL to 1 μg/mL. Moreover, the proposed aptasensor had excellent specificity, outstanding repeatability and favorable stability. Finally, the proposed ECL aptasensor was used for the detection of profenofos in real samples and the recoveries ranged from 94% to 102%. This strategy could provide a good reference for developing effective aptasensors for detection of other small molecules and toxins.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Guangyan Liu,Jun Ling,Hanzhao Xie,Jianping Li
A molecularly imprinted polymer (MIP)-electrochemiluminescence (ECL) sensor was prepared and an ultrasensitive strategy was proposed for detecting an ultra-trace amount of ethiprole based on the enzymatic signal amplification of glucose oxidase (GOD). The ethiprole molecule on the MIP was marked with GOD-encapsulated liposomes via light-triggered click reaction after elution and readsorption. Many GOD particles were released from the liposomes and catalyzed the reaction to produce a large amount of H 2 O 2 in the presence of glucose, which considerably enhanced the ECL intensity of luminol. Therefore, the sensitivity for ethiprole determination was promoted remarkably. The ECL signal of the sensor was linearly related to the logarithm of ethiprole concentration from 5.00 × 10 −16 M to 5.00 × 10 −11 M, and the detection limit was 1.00 × 10 −16 M, which was the lowest of all the analytical methods used in ethiprole determination. Ethiprole in samples was determined directly without the tedious and complicated sample treatment procedures, such as separation and enrichment. The click chemistry labeling method using liposomes simplified the operation steps of the MIP-ECL sensor in ethiprole determination.
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