Biomedicines
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2023
·Vol. 11
·期 10
·2859
·
IF 4.7
Chanhao Lei,Zihan Gao,Xingzhi Lv,Yanxue Zhu,Ruifang Li,Sanqiang Li
The development of primary liver cancer (PLC) is associated with chronic liver inflammation and the loss of associated tumor suppressor genes, which characterizes inflammation-related tumors. In this study, we aimed to explore the effect of saikosaponin-b2 (SS-b2) on the development of PLC and its effect of the STK4 expression and IRAK1/NF-κB signaling axis. In vitro and in vivo experiments showed that SS-b2 exerted potent anti-inflammatory and antitumor effects. A PLC model was induced in vivo by treating male BALB/c mice with diethylnitrosamine, while an inflammatory model was induced in vitro by exposing RAW 264.7 macrophages to lipopolysaccharides (LPS). After treating cancer mice with SS-b2, the serum levels of alpha-fetoprotein, aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase significantly reduced. Ki67 expression also decreased. The carcinomatous lesions of the liver were attenuated. Similar results were observed in liver tissue and RAW 264.7 macrophages, where SS-b2 significantly elevated serine/threonine protein kinase 4 (STK4) expression and decreased the expression of interleukin-1 receptor–associated kinase 1 (IRAK1), nuclear factor-kappaB (NF-κB), and downstream inflammatory cytokines, thus exerting anti-cancer and anti-inflammatory effects. Moreover, we employed siRNA to silence the STK4 expression in HepG2 to investigate the anti-tumor effect of SS-b2 in vitro. The STK4 knockdown would upregulate IRAK1 and thus the activation of NF-κB activity revealed by the increase in the levels of proinflammatory cytokines, consequently impairing SS-b2-induced inhibition of liver cancer development. Consequently, SS-b2 effectively inhibited PLC by upregulating STK4 to suppress the IRAK1/NF-κB signaling axis and is a promising agent for treating this disease.Keywords:primary liver cancer;saikosaponin-b2;serine/threonine protein kinase 4;interleukininterleukin-1 receptor-associated kinase 1;nuclear factor-kappa B
研究领域:
肿瘤学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
·
2025
·
IF 2.6
Yao Wang,Pan Wang,Fangfang Xu,Han Zhou,Xiaomin Xie,Tao Hou,Fengbin Luo,Yuchang Sun,Yanfang Liu,Jixia Wang,Dongmei Fu
ATP citrate lyase (ACLY) is a crucial enzyme in glycolipid metabolism. To date, only its one prodrug bempedoic acid has been approved. The discovery of direct ACLY inhibitors is important for developing new lipid-lowering drugs. Grape seeds, used in functional foods and medicines, have lipid-lowering efficacy. However, it remains unknown whether their components directly inhibit ACLY. To investigate it, a multi-component screening method was used and proanthocyanidin B2 was identified as a novel ACLY inhibitor with an IC 50 value of 2.73 μM. Differential scanning fluorimetry confirmed its directly binding to ACLY. Proanthocyanidin B2 acted as a noncompetitive inhibitor in kinetic studies and bound stably with ACLY in molecular dynamics simulations. In vitro , proanthocyanidin B2 reduced lipid accumulation via inhibiting key transcription factors associated with ACLY. This study revealed a novel ACLY inhibitor contributing to lipid-lowering efficacy of grape seeds and provided a unique scaffold for ACLY drug design.
研究领域:
生物化学
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药理学
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分子生物学
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天然产物化学
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代谢疾病
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药物发现
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产品关联
DOI
PubMed
高影响因子
Science China-Materials
·
2023
·Vol. 66
·期 8
·3213-3222
·
IF 8.1
Wen Zihao,Meng Hong,Jiang Shengda,Tang Zhongyu,Liu Yiwen,Chu Yanhui
Developing superior oxidation resistance of high-entropy diborides is critical for extending their potential applications in harsh environments. Herein, we developed non-equimolar (Hf,Zr,Ta,W)B 2 high-entropy diborides with superior oxidation resistance by adjusting W contents for the first time. The as-fabricated (Hf 0.28 Zr 0.28 Ta 0.28 W 0.15 )B 2 samples are predicted to possess superior oxidation resistance at 1473–1773 K through the oxidation depth quantitative analysis obtained by machine learning, which is attributed to the reason that the moderate WO 3 can effectively inhibit the volatilization of B 2 O 3 . Furthermore, a four-layered structure is found in the generated product layers at 1773 K, which is due to the preferential oxidation of Hf and Zr elements at low oxygen partial pressure and the different diffusion activation energies of oxide products. The preferential oxidation of Hf and Zr elements is further demonstrated by the computational phase stability diagrams, and oxygen adsorption energies and charge transfer between oxygen atoms and different metal adsorption sites via first-principles calculations. Such superior performance endows (Hf 0.28 Zr 0.28 Ta 0.28 W 0.15 )B 2 with potential applications as ultrahigh-temperature structural materials.
研究领域:
材料科学
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高温材料
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计算材料科学
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产品关联
DOI
高影响因子
Chemosensors
·
2022
·Vol. 10
·期 5
·154
·
IF 4.229
Fan Jia,Yuye Li,Qingfa Gong,Dong Liu,Shuyun Meng,Chengxi Zhu,Tianyan You
The accurate and reliable analysis of aflatoxin B2 (AFB2) is widely required in food and agricultural industries. In the present work, we report the first use of a ratiometric electrochemical aptasensor for AFB2 detection with high selectivity and reliability. The working principle relies on the conformation change of the aptamer induced by its specific recognition of AFB2 to vary the ratiometric signal. Based on this principle, the proposed aptasensor collects currents generated by thionine–graphene composites (ITHI) and ferrocene-labeled aptamers (IFc) to output the ratiometric signal of ITHI/IFc. In analysis, the value of ITHIremained stable while that of IFcincreased with higher AFB2 concentration, thus offering a “signal-off” aptasensor by using ITHI/IFcas a yardstick. The fabricated aptasensor showed a linear range of 0.001–10 ng mL−1with a detection limit of 0.19 pg mL−1for AFB2 detection. Furthermore, its applicability was validated by using it to detect AFB2 in peanut and peanut oil samples with high rates of recovery. The developed ratiometric aptasensor shows the merits of simple fabrication and high accuracy, and it can be extended to detect other mycotoxins in agricultural products.Keywords:ratiometry;electrochemical aptasensor;thionine;aflatoxin B2
研究领域:
食品科学
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分析化学
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产品关联
DOI
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2018
·
IF 2.568
Lu Zeng,Wei Jiang,Liqiang Liu,Shanshan Song,Hua Kuang
Vitamin B2 (riboflavin) is a water-soluble vitamin that has important roles in human health. In this study, we developed a sensitive and specific monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and lateral-flow immunochromatographic assay (ICA) strip for the rapid detection of vitamin B2. Following routine fusion and selection, the optimum monoclonal antibody against vitamin B2 was obtained. The 50% inhibitory concentration and limit of detection of ic-ELISA were 8.18 and 1.80 ng/mL, respectively. The cut-off value of the lateral-flow ICA strip was 50 ng/mL. The results revealed that our developed methods are suitable for the on-site detection and mass screening of vitamin B2 in food and pharmaceutical products.
研究领域:
分析化学
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免疫学
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食品科学
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产品关联
DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 166
·期 10
·B821
·
IF 3.12
Pengfei Li,Zhenping Liu,Zhibin Yan,Xin Wang,Eser Metin Akinoglu,Mingliang Jin,Guofu Zhou,Lingling Shui
Insufficient or excessive consumption of Vitamin B2 (Vit B2) and Vitamin B6 (Vit B6) can cause harmful effects on human health conditions, it is therefore very important to control their content in supplements and food products. In this work, we report on a simple, sensitive and selective electrochemical sensor based on gold nanoparticles (AuNPs) on glassy carbon electrode (GCE) modified by a hybrid of polydopamine and reduced graphene (PDA-RGO), for determination of Vit B2 and Vit B6. The hybrid nanomaterial comprising PDA-RGO supported AuNPs is constructed through a one-step GCE modification process for electrochemical quantification of Vit B2 and Vit B6 in pharmaceutical formulations. Cyclic voltammetry result reveals that the effective response to Vit B2 and Vit B6 is according to the high electrical conductivity of AuNPs and the large surface area of PDA-RGO. Meanwhile, the sensor modified with a composite film shows a synergistic effect for catalyzing the analytes. Under optimized experimental conditions, these sensors show wide linear ranges of 0.02−60.0 μM and 0.03−600.0 μM, with the detection limits (LODs) of 0.0096 μM and 0.025 μM for Vit B2 and Vit B6, respectively. Additionally, the constructed sensors have been applied for quantifying Vit B2 and Vit B6 in pharmaceutical formulations. Such an effective electrochemical platform could be potentially applied for analyzing various components for therapeutic drug monitoring.
研究领域:
分析化学
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生物医学工程
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药物分析
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DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2023
·
IF 6
Zhen Zhang,Limin Wang,Qian Zhang,Hongkun Li,Yong Xiang,Xinyu Wang,Xiaoran Hu
Chronic wounds pose an increasing burden not only to patients, but also to the health system. Wound repair requires the timely and orderly migration of fibroblasts to the wound bed. In this respect, electrical stimulation by a piezoelectric generator (PEG) is an attractive approach to wound management because it is noninvasive and effective in promoting chronic wound healing. However, commonly used PEGs based on polyvinylidenefluoride (PVDF) and poly( l -lactic acid) (PLLA) suffer from low biocompatibility and low piezoelectric output, respectively. Herein, we developed a bio-based PEG composed of PLLA blended with vitamin B 2 (VB 2 ), leading to an improvement of crystallinity and β-phase orientation, which exhibited better biocompatibility than PVDF and a higher piezoelectric output than pure PLLA. Such piezoelectricity could serve as effective electrical stimulation and promoted in vitro cell migration and fibroblast proliferation, evidenced by significant higher gene expression levels of extracellular matrix proteins and growth factors. Moreover, it facilitated in vivo wound closure by enhancing the re-epithelialization, collagen deposition, neovascularization, and increase the concentration of growth factors in the wound bed, leading to a 1.4-fold higher wound closure rate than that in the control group. Thus, the PLLA/VB 2 PEG opens an avenue for the application of bio-based PEGs for wound healing.
研究领域:
生物医学工程
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材料科学
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组织工程
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伤口愈合
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产品关联
DOI
高影响因子
Polymers
·
2025
·Vol. 17
·期 14
·1909
·
IF 4.9
Andrey Galukhin,Roman Aleshin,Alexander Gerasimov,Alexander Klimovitskii,Roman Nosov,Liana Zubaidullina,Sergey Vyazovkin
Non-isothermal calorimetry is performed to study the kinetics of metal-free A2/B2-type azide–alkyne polyaddition between the dipropargyl ether of bisphenol A with different organic diazides. The diazide structure is varied to probe the effect of the nature of a hydrocarbon spacer between the azide groups on their reactivity. Isoconversional analysis demonstrates that the polymerization processes are characterized by the same activation energy of 84 kJ mol−1for all studied diazides. It is found that diazides with aromatic spacers demonstrate ~1.6 times higher reactivity than that of diazides with the alkyl spacer. The difference in the reactivity is explained by the difference in the electronic effects of the hydrocarbon spacers on the azide groups as well as by the difference in their steric availability. The veracity of the obtained kinetic parameters is validated by a polymerization test at the time–temperature conditions predicted from the obtained kinetic data followed by independent assessment of the monomer conversion using FTIR.
研究领域:
高分子化学
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物理化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Xinmei Wang,Shanshan Li,Jiayu Yu,Wenlin Wang,Zhuoqi Du,Shuchun Gao,Yin Ma,Ruixin Tang,Ting Liu,Shiping Ma,Qiang Fu,Xueyang Deng
s Ethnopharmacological relevance Saikosaponins B2 (SSB2) is one of the main active components isolated from Radix Bupleuri (Bupleurum chinense DC.), a herb widely used of traditional Chinese medicine. It has been used for the treatment of depression for more than two thousand years. However, the molecular mechanisms remain to be determined. Aim of the study In this study, we evaluated the anti-inflammatory effect and elucidated underlying molecular mechanisms of SSB2 in LPS-induced primary microglia and CUMS-induced mice model of depression. Method The effects of SSB2 treatment were investigated both in vitro and in vivo. The chronic unpredictable mild stimulation (CUMS) procedure was applied to establish the animal model of depression. Behavioural tests were used to evaluate the depressive-like behaviors in CUMS-exposed mice, including sucrose preference test, open field test, tail suspension test, and forced swimming test. The GPX4 gene of microglia was silenced using shRNA, and inflammatory cytokines were determined by Western Blot and immunofluorescence analysis. Endoplasmic reticulum stress and ferroptosis-related markers were detected by qPCR, flow cytometry and confocal microscopy. Result SSB2 reversed depressive-like behaviours in CUMS-exposed mice and relieved central neuroinflammation and ameliorated hippocampal neural damage. SSB2 alleviated LPS-induced activation of microglia through the TLR4/NF-κB pathway. LPS-induced ferroptosis, with increased levels of ROS, intracellular Fe 2+ , mitochondrial membrane potential, lipid peroxidation, GSH, SLC7A11, FTH, GPX4 and Nrf2, and decreased transcription levels of ACSL4 and TFR1, was attenuated with SSB2 treatment in primary microglia cells. GPX4 knockdown activated ferroptosis, induced endoplasmic reticulum (ER) stress, and abrogated the protective effects of SSB2. Further, SSB2 attenuated ER stress, balanced calcium homeostasis, reduced lipid peroxidation and intracellular Fe 2+ content by regulating the level of intracellular Ca 2+ . Conclusions Our study suggested that SSB2 treatment can inhibit ferroptosis, maintain calcium homeostasis, relieve endoplasmic reticulum stress and attenuate central neuroinflammation. SSB2 exhibited anti-ferroptosis and anti-neuroinflammatory effects through the TLR4/NF-κB pathway in a GPX4-dependent manner.
研究领域:
药理学
·
神经科学
·
民族药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Daojiu Zhang,Xu Yang,Yichun Wang,Biao Wang,Shaoyu Wang,Jinyang Chang,Suwen Liu,Hao Wang
We investigated the roles of Proanthocyanidin B2 (PCB2) and transglutaminase (TGase) in improving myofibrillar protein (MP) gel properties. TGase and PCB2 increased the surface hydrophobicity (41 %) and water holding capacity (WHC) (16 %) of the MP. Secondary and tertiary structures of MP were respectively analyzed by Fourier transform infrared spectroscopy (FTIR) and fluorescence spectrophotometry. The content of α -helix in MP was found to be increased while its fluorescence intensity decreased upon the addition of PCB2 and TGase. The addition of PCB2 and TGase resulted in formation of a dense MP network structure as revealed by scanning electron microscopy (SEM). Low-field nuclear magnetic resonance (LF-NMR) and magnetic resonance imaging (MRI) analysis showed that immobilized water levels in the MP gel were markedly increased while the amount of free water was significantly decreased after PCB2 and TGase addition. These findings indicate that a combination of PCB2 and TGase are potential additives for meat products.
研究领域:
食品科学
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蛋白质化学
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生物物理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2022
·Vol. 105
·期 10
·6370-6383
·
IF 4.186
Yu Gao,Liang Huang,Zhaoming Tong,Jianghao Liu,Qi Zhang,Haijun Zhang,Quanli Jia,Shaowei Zhang
A theoretical calculation combined with experiment was used to study high-entropy (Hf 0.2 Ti 0.2 Mo 0.2 Ta 0.2 Nb 0.2 )B 2 (HEB-HfTiMoTaNb). The theoretical calculation suggested HEB-HfTiMoTaNb could be stable over a wide temperature range. Then, a novel solvothermal/molten salt-assisted borothermal reduction method was proposed to efficiently pre-disperse transitional metal atoms in a precursor and synthesize (Hf 0.2 Ti 0.2 Mo 0.2 Ta 0.2 Nb 0.2 )B 2 nanoscale powders at 1573 K for 6 h, which is nearly 300 K lower than previous reports. The characterization results indicated that the as-synthesized nanoscale HEB-HfTiMoTaNb powder was hexagonal single-phase with homogeneous elements distribution and uniform size, and the oxygen content of the particles is 0.97 wt%. Simultaneously, the mechanical properties, anisotropic nature, and thermal properties of HEB-HfTiMoTaNb were investigated by density functional theory (DFT) calculations. The Cannikin's law was adopted to explain the improvement of comprehensive mechanical properties. In addition, a significant reduction of thermal conductivity was observed for HEB-HfTiMoTaNb and it only was 1/15 of the value of HfB 2 . This work suggests a reliable technique for synthesis of nanosized HEB powders and discovery of high-entropy materials under the guidance of first-principle theory.
研究领域:
材料科学
·
物理化学
·
理论物理
✓ 已验证
DOI
高影响因子
Journal of Functional Foods
·
2018
·
IF 3.47
Hong Ding,Xiaofei Li,Jian Li,Zhike Li,Yunhui Niu,Wanying Ren,Jianyang Tan,Shanye Yin
The present study examined the effects of procyanidin B2 (PB2) on L-NAME (nitric oxide synthase inhibitor)-induced hypertensive nephropathy. After 4 weeks of treatment with daily PB2 supplementation, lower blood pressure levels were observed in rats. The potential mechanisms were combined with amelioration of renal function impairment. The L-NAME–induced renal histological damage were reduced by PB2 treatment, which showed similar protective tendency in kidney injury molecule–1, nephrin, fibronectin and α–smooth muscle actin protein expression. This appears to be due to PB2 inducing a less pronounced generation of renal reactive oxygen species and inflammatory mediators than in the model group. Downregulation of Ang II type 1 receptor and NADPH oxidase subunit (NOX4, gp91phox and p47phox) played a critical role in the effect of PB2 reducing oxidative stress production. These activities could further support the use of PB2 commercially in food for the prevention of hypertension and its complications.
研究领域:
药理学
·
肾病学
·
高血压研究
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）