Microorganisms
·
2026
·Vol. 14
·期 3
·647
·
IF 4.7
Qing Wang,Yucheng Cao,Yunna Xu,Keng Yang,Chuangwen Xu,Guoliang Wen,Jinfan Liu,Jianshe Zhang,Xiaojuan Hu
Biologically controllingMicrocystis aeruginosablooms in saline–alkaline environments remains a major challenge in aquatic ecosystem management. Here, the algicidal performance of an indigenous algicidal bacterium,Bacillus cereusstrain PT1 isolated from a sulfate-type saline–alkaline pond, againstM. aeruginosawas evaluated, and the underlying metabolic mechanisms were elucidated using non-targeted metabolomics. PT1 exhibited pronounced, stable algicidal activity under saline–alkaline conditions, decreasing the algal cell density from 2 × 106to 1.25 ± 0.5 × 105cells mL−1within 4 days at a rate of 93.75 ± 2.5% (p< 0.05). The above results demonstrate that strain PT1 has a significant lytic effect onM. aeruginosa. Non-targeted liquid chromatography–mass spectrometry analysis identified 298 PT1-induced accumulated metabolic features, and the top 30 candidates comprised organic acids and aromatic compounds, including benzoic acid, coumarin, malonic acid, and signaling-related molecules, including indoleacetaldehyde and nitroprusside. These differential metabolites were associated with algicidal-related pathways, including quorum sensing, two-component systems, ABC transporters, and tryptophan metabolism, outlining a coordinated “regulation–transport–metabolic remodeling” framework. Our findings demonstrate the potential of the indigenous algicidal strain PT1 from saline–alkali ponds to controlM. aeruginosablooms. They also provide an important theoretical basis and data foundation for further elucidating the molecular characteristics of algae solubilizing activity under saline–alkali conditions and developing microbial agents for preventing and controllingMicrocystisblooms in saline–alkali ponds.
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PubMed
高影响因子
Food Analytical Methods
·
2021
·Vol. 14
·期 9
·1964-1976
·
IF 3.366
Chen Guanhua,Yang Guotai,Wang Yutong,Deng Mei,Wang Zhengzheng,Aguilar Zoraida P.,Xu Hengyi
Sensitive, simple, and rapid pathogen separation and detection methods are essential for the early diagnosis and immediate medical treatment of bacterial infections. In this study, a novel strategy based on vancomycin (Van)-modified ovalbumin (OVA) mediated magnetic nanoprobes (MBs-OVA-Van) combined with multiplex polymerase chain reaction (mPCR) was developed for efficient isolation and detection of Staphylococcus aureus ( S. aureus ) and Bacillus cereus ( B. cereus ). Van conjugated to ovalbumin coated magnetic microcarrier via strong covalent interaction. In addition, Van binds through hydrogen bonds with d -alanyl- d -alanine moieties on the surface of gram-positive bacteria cell wall. Under optimum conditions, the MBs-OVA-Van showed that the capture efficiency (CE) for S. aureus and B. cereus in PBS and juice samples were around 91%, 92% and 89%, 85%, respectively. In addition, with a mixture containing both target bacteria in PBS and juice samples, the CE for S. aureus and B. cereus were recorded at about 90%, 91% and 80%, 84%, respectively. The MBs-OVA-Van plus mPCR method applied in PBS and juice samples for both bacteria showed limits of detection at 10 2 CFU mL -1 in a < 3 h total assay time. The results indicated that the method holds promise for isolation and enrichment of gram-positive bacteria with S. aureus and B. cereus as models.
研究领域:
微生物学
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生物技术
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分子生物学
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分析化学
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高影响因子
ACS Synthetic Biology
·
2025
·
IF 3.9
Jing Wu,Ziqian Xiao,Yuhui Du,Minglan Luo,Jiacheng Zhang,Pei Guo,Zhipeng Chen,Binyuan Yang,Tingjian Chen
Aptamer-based sensors (aptasensors) have been extensively employed in biosensing and diagnostics. However, the broader application of aptasensors fabricated with DNA and RNA aptamers has been limited due to the intolerance of DNA and RNA to nucleases in biological fluids. A majority of sugar-modified nucleic acids, in particular 2’-F and 2’-OMe-modified nucleic acids, demonstrate a remarkable resistance to nuclease degradation and thus hold great potential in the development of more stable aptamers and aptasensors. In this work, employing enzymes including a laboratory-evolved polymerase for 2’-modified nucleic acids, we carried out systematic evolution of ligands by exponential enrichment (SELEX) experiments to obtain 2’-F/OMe-RNA aptamers for Bacillus cereus 5/B/6 metallo-β-lactamase (B. cereus 5/B/6 MBL), a crucial marker of many penicillin-resistant strains. Three aptamers composed of 2’-OMe-A, 2’-F-U, 2’-F-C, and 2’-OMe-G nucleotides (Apt-FUC1, Apt-FUC2, and Apt-FUC3) have been obtained and systematically characterized. All of them exhibited excellent target binding affinities, with equilibrium dissociation constant (Kd ) values of 95.9, 70.0, and 81.3 nM, and outstanding biological stability. Combining FAM-labeled Apt-FUC1, Apt-FUC2, and Apt-FUC3 with graphene oxide (GO), which could quench the fluorescence of FAM, we successfully constructed three sensitive and biologically stable 2’-F/OMe-RNA aptasensors for B. cereus 5/B/6 MBL, with the limit of detection (LOD) values of 0.38, 0.27, and 0.29 nM. Moreover, these three aptasensors all demonstrated good specificity for target detection in biological samples. This work has established a streamlined strategy for generating stable 2’-F/OMe-RNA aptamers and aptasensors, which should broaden the application of sugar-modified nucleic acids in biosensing and diagnostics.
研究领域:
分子诊断
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核酸工程
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生物传感器
·
酶检测
·
抗生素耐药性
·
合成生物学
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PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Yanmei Li,Weilong Li,Zigan Xu,Zixi Chen,Zhuoheng Song,Yeye Yu,Ting Xie,Yong Dai,Xiehui Chen,Shaodong Luan,Hanchao Gao
Bacillus cereus is a common spoilage microorganism that can survive in various foods and produce toxins, causing food poisoning such as diarrhea and vomiting, and seriously threatening public health and food safety. Therefore, developing efficient and safe control methods is of great significance for ensuring food quality. In this study, we identified and characterized a novel cell wall lytic enzyme, LysM15, derived from the genome of B. cereus VD045. Structural analysis showed that LysM15 contains an M15 catalytic domain and an SH3 binding domain. Recombinant LysM15 exhibited strong and specific lytic activity against B. cereus , with no effect on other pathogenic species, achieving rapid bacterial lysis within 10 min at 0.008 g/L. At 0.0125 g/L, it also effectively inhibited spore germination and eliminated preformed biofilm. Moreover, the enzyme maintained high lytic activity under a variety of physicochemical conditions, including pH 7.0–9.0, 50 °C, and 0.3 mol/L NaCl. Mechanistic investigations showed that LysM15 disrupted the cell wall, caused cytoplasmic leakage and structural collapse, and interfered with metabolism and gene expression, ultimately leading to bacterial death. Notably, LysM15 significantly decreased B. cereus counts in beef samples stored at 4 °C. These findings suggest that LysM15 possesses strong, specific, and stable antibacterial properties, highlighting its potential as an effective biocontrol agent against B. cereus contamination in food systems.
研究领域:
微生物学
·
食品安全
·
酶学
·
基因组学
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代谢组学
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2018
·
IF 6.434
Feng Li,Wei Wang,Chengcheng Li,Runliang Zhu,Fei Ge,Yang Zheng,Yixin Tang
Biomineralization is an interesting naturally occurring process of forming minerals by microorganisms, which offers an efficient way to sequester heavy metal ions within relatively stable solid phases. In this study, Bacillus cereus Cd01 was selected to investigate effects of self-mediated pH on biosorption and biomineralization of Cd 2+ in whole 72h cultivation period. Results revealed that strain Cd01-mediated pH decrease of the cultivation medium from 7.0 to 6.1 inhibited biosorption of Cd 2+ on Cd01 cells at the initial cultivation period, while an increased pH from 6.1 to 7.4 facilitated biosorption of Cd 2+ on Cd01 cells at the middle and late cultivation period. The reasons were mainly that self-mediated pH altered cell surface hydrophobicity and cell membrane fluidity of strain Cd01. Moreover, biosorption and bioaccumulation of Cd 2+ on Cd01 cells in the period of increased pH promoted biomineralization of Cd 2+ observed by the transmission and scanning electron microscopes. The analyses of energy dispersive spectroscopy, X-ray photoelectron spectroscopy and select area electron diffraction demonstrated that Cd 2+ loaded on Cd01 cells was biomineralized into polycrystalline and/ or amorphous cadmium sulfide and cadmium phosphate. These results suggest that strain Cd01 may play a potential role in biomineralization remediation of heavy metal contaminated soils.
研究领域:
微生物学
·
环境科学
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PubMed
高影响因子
FOOD MICROBIOLOGY
·
2024
·
IF 4.5
Yang Liu,Wenwen Ge,Yuxin Sun,Hongchao Dai,Luyao Fan,Lei Yuan,Zhenquan Yang,Xinan Jiao
Bacillus cereus and Bacillus licheniformis are widely presented in dairy products. They can form thick biofilms on surfaces of dairy processing equipment, which may pose serious safety issues and spoilage of final dairy products. However, how ecological interactions between B. cereus and B. licheniformis affect the functions and stability of mixed-species biofilm remains uncovered. In this work, the altered profiles of a dual-species biofilm by dairy-derived B. cereus 121 and B. licheniformis 919 were investigated by RNA-sequencing analysis in combined with phenotype validation (bacterial growth, biofilm-forming capacity, biofilm EPS production, and biofilm structures). The results confirmed that the presence of B. cereus 121 reduced the growth of B. licheniformis 919 planktonic cells, and decreased the biofilm cell numbers of B. licheniformis 919 in the dual-species biofilm when compared to that in its single-species biofilm. The bacterial interaction also reduced the amount of proteins and carbohydrates in the biofilm matrix, and decreased the coverage, average thickness, and total biomass of biofilms. In addition, results from RNA-sequencing analysis showed that the bacterial interaction caused a total of 128 ( B. licheniformis 919) and 216 ( B. cereus 121) differentially expressed genes (DEGs) during the co-culture of planktonic cells. Functional annotation revealed that the DEGs of B. licheniformis 919 were mainly involved in 10 downregulated pathways including citrate cycle, pyruvate metabolism, nonribosomal peptide structures, glycolysis/gluconeogenesis, quorum sensing, alanine, aspartate and glutamate metabolism, oxidative phosphorylation, beta-Lactam resistance, arginine and proline metabolism, and beta-Alanine metabolism when co-cultured with B. cereus 121. On the other hand, the DEGs from B. cereus 121 were significantly enriched for two downregulated pathways (cysteine and methionine metabolism, and inositol phosphate metabolism) and four upregulated pathways (nitrogen metabolism, glyoxylate and dicarboxylate metabolism, glycine, serine and threonine metabolism, and propanoate metabolism). Results of this study facilitate updated knowledge of how bacterial interaction during the biofilm formation shapes the features of the mixed-species biofilm.
研究领域:
微生物学
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食品科学
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分子生物学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2024
·
IF 5
Yanmei Li,Lun Luo,Wenhai Wang,Bin Hong,Yi Ma,Jufang Wang
Bacillus cereus is a prevalent foodborne pathogen that induces food poisoning symptoms such as vomiting and diarrhea. Its capacity to form spores and biofilm enables it to withstand disinfectants and antimicrobials, leading to persistent contamination during food processing. Consequently, it is necessary to develop novel and efficient antimicrobial agents to control B. cereus , its spores, and biofilms. Peptidoglycan hydrolases have emerged as a promising and eco-friendly alternative owing to their specific lytic activity against pathogenic bacteria. Here, we identified and characterized a Lysozyme-like cell wall hydrolase Lys14579, from the genome of B. cereus ATCC 14579. Recombinant Lys14579 specifically lysed B. cereus without affecting other bacteria. Lys14579 exhibited strong lytic activity against B. cereus , effectively lysing B. cereus cell within 20 min at low concentration (10 μg/mL). It also inhibited the germination of B. cereus spores and prevented biofilm formation at 12.5 μg/mL. Moreover, Lys14579 displayed good antimicrobial stability with negligible hemolysis in mouse red blood cells and no cytotoxicity against RAW264.7 cells. Notably, Lys14579 effectively inhibited B. cereus in boiled rice and minced meat in a dose-dependent manner. Furthermore, bioinformatics analysis and point mutagenesis experiments revealed that Glu-47 was the catalytic site, and Asp-57, Gln-60, Ser-61 and Glu-63 were active-site residues related with the cell wall lytic activity. Taken together, Lys14579 could be a promising biocontrol agent against vegetative cells , spores, and biofilm of B. cereus in food industry.
研究领域:
微生物学
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食品科学
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生物技术
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PubMed
高影响因子
Biotechnology Journal
·
2018
·Vol. 14
·期 3
·1800253
·
IF 3.507
Junping Zhou,Yaling Wang,Jiajie Chen,Meijuan Xu,Taowei Yang,Junxian Zheng,Xian Zhang,Zhiming Rao
Unnatural amino acids (UAAs) play a key role in modern medicinal chemistry such as small molecules and peptide-based drugs with fast-growing markets. Low efficiency for natural enzymes including leucine dehydrogenase (LeuDH, EC1.4.1.9) are one major challenge for UAA production. Here, rational engineering of LeuDH from Bacillus cereus with a structure-based design approach is studied. The results achieve higher enzymatic activity and stability toward α-keto acid reduction by improving the hydrophobic and rigidity of enzymatic substrate entrance tunnel. High catalytic efficiency for variant E116V is associated with the presence of more hydrophobic tunnels that allows easy substrate diffusion, which is confirmed in absorbance spectroscopy study. For variant T45M/E116V, melting temperature and half-lives of thermal inactivation at 60 °C is 62.8 °C and 29.2 h, respectively, much higher than 48.4 °C and 3.4 h of wild type. Structural analysis indicates that an additional hydrogen bond in β5 fold is formed in variant T45M, which results in a more rigid β5 fold leading to better stability. Furthermore, asymmetric synthesis of α-amino acids with coenzyme regeneration reveals higher productivities for variant T45M/E116V. This study indicates the importance of substrate entrance tunnel for enzymatic activities and stability, the engineered LeuDH would better serve UAA production. Graphical Low yield rates for natural enzymes including leucine dehydrogenase (LeuDH, EC1.4.1.9) are one major challenge to the production of unnatural amino acids in industrial scale. Here, the catalytic activity and stability of Bc LeuDH toward α-keto acid reduction are remarkably improved via rational engineering of substrate entrance tunnel based on a structure-based design approach. This work will better serve the production of especially unnatural α-amino acids.
研究领域:
生物化学
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酶工程
·
药物化学
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生物催化
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PubMed
高影响因子
Microorganisms
·
2020
·Vol. 8
·期 11
·1750
·
IF 4.152
Chun Li,Xiaoming Yuan,Na Li,Juan Wang,Shubo Yu,Haiyan Zeng,Jumei Zhang,Qingping Wu,Yu Ding
Bacillusphage φ29 and its relatives have been considered as one of the most important model organisms for DNA replication, transcription, morphogenesis, DNA packaging studies, and nanotechnology applications. Here, we isolated and characterized a new member of the φ29-like phage, namedBacillus cereusphage vB_BceP-DLc1. This phage, with a unique inserted gene cluster, has the largest genome among known φ29-like phages. DLc1 can use the surface carbohydrate structures of the host cell as receptors and only infects the most relatedB. cereusstrains, showing high host-specificity. The adsorption rate constant and life cycle of DLc1 under experimental conditions were also determined. Not only stable under temperatures below 55 °C and pH range from 5 to 11, the new phage also showed tolerance to high concentrations of NaCl, 75% ethanol, chloroform, and mechanical vortex, which is preferable for practical use in the food and pharmaceutical industries.Keywords:Bacillus cereus;bacteriophage;φ29;stability;adsorption;largest genome
研究领域:
微生物学
·
分子生物学
·
病毒学
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PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2015
·Vol. 178
·期 8
·1471-1480
·
IF 1.735
Zheng Jian-yong,Liu Yin-yan,Luo Wei-feng,Zheng Ren-chao,Ying Xiang-xian,Wang Zhao
A new esterase-producing strain ( Bacillus cereus WZZ001) which exhibiting high hydrolytic activity and excellent enantioselectivity on rac -α-ethyl-2-oxo-pyrrolidineacetic acid methyl ester ( R , S - 1 ) has been isolated from soil sample by our laboratory. In this study, the stereoselective hydrolysis of ( R , S - 1 ) was performed using the recombinant Bacillus cereus esterase which expressed in Escherichia coli BL21 (DE3). Under the optimized conditions of pH 8.0, 35 °C, and concentration of substrate 400 mM, a successful enzymatic resolution was achieved with an e.e.
s of 99.5 % and conversion of 49 %. Immobilization considerably increased the reusability of the recombinant esterase; the immobilized enzyme showed excellent reusability during 6 cycles of repeated 2 h reactions at 35 °C. Thereby, it makes the recombinant B. cereus esterase a usable biocatalyst for industrial application.
研究领域:
生物化学
·
生物技术
·
酶学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2022
·
IF 5.911
Haiying Cui,Jingyu Lu,Changzhu Li,Marwan M.A. Rashed,Lin Lin
Bacillus cereus ( B. cereus ) is a recognized foodborne pathogen widely distributed in various protein-rich foods , which is a huge challenge to food safety. Herein, a novel enzyme-responsive nanomaterial based on cationic starch (CSt) nanofibers loaded with carvacrol@casein nanoparticles (CL@CSNPs) was constructed (CL@CS/CSt nanofiber) to prevent the contamination of B. cereus in soybean products. Considering the excellent antibacterial activity of carvacrol (CL) against B. cereus , CL@CSNPs were prepared by electrostatic adsorption and hydrophobic interaction and characterized by SEM and FTIR .CL@CS/CSt nanofibers with better performance were determined by comparing the physical properties of the electrospinning solution and the prepared nanofiber. Nanofibers were prepared by electrospinning technology and analyzed by SEM and AFM to investigate the size and structural morphology of fibers. FTIR analyses were done to confirm the successful embedding of CL@CSNPs in CSt nanofibers. Subsequently, the controlled release of CL was verified by GC–MS and disc diffusion method. The application experiment results indicated that the treatment based on CL@CS/CSt nanofibers reduced the B. cereus in soy products by 2 log CFU/g, which reflected a significant antibacterial activity. In addition, CL@CS/CSt nanofibers could also prevent texture and chroma changes under refrigeration and maintain the sensory quality of soy products. Thus, CL@CS/CSt nanofibers appear to have great potential in controlling the contamination of soybean products by B. cereus while maintaining the physical quality.
研究领域:
食品科学
·
微生物学
·
材料科学
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Qiuting Yan,Yalin Yang,Wei Chen,Xiulan Weng,Gary Owens,Zuliang Chen
The efficient removal and recovery of rare earth elements (REEs) from mine wastewater is highly desirable due to the increasing public demands to both protect the environment and recover valuable REEs resources. In this study, bio-nanoparticles ( BC @FeNPs-EPS), which combined extracellular polymeric substances (EPS) and iron nanoparticles (FeNPs) were synthesized via Bacillus cereus and used to remove REEs such as Eu(III) from mine wastewaters. The removal efficiency of BC @FeNPs-EPS (90.2%) was consistently higher than the removal efficiencies of the three constituent components alone, FeNPs (85%), Free cell (67%) and EPS (10%) exhibited. SEM and XPS analysis indicated the existence of substantial organic capping agents derived from microorganisms on the FeNPs, where comparison of FTIR spectra before and after exposure to Eu(III) showed changes in both wavenumber and peak intensity of these various organic functional groups suggesting the formation of surface complexes with Eu(III), whereas Zeta potential measurements of the material also supported electrostatic interactions between Eu(III) and BC @FeNPs-EPS. Adsorption kinetics almost perfectly correlated with the non-linear pseudo-second-order model. Collectively, these results indicated that the removal mechanism for Eu(III) by BC @FeNPs-EPS involved both surface complexation and ion exchange and the additive effect removal of Eu(III) by FeNPs, Free cell and EPS. In addition, the practical removal efficiency of a range of REEs from real mine wastewaters by BC @FeNPs-EPS was consistently high being 88% for Eu(III), 89% for Tb(III) and 89% for Ce(III). The material produced was also highly reusable, maintaining a high Eu(III) removal rate (69%) even after 5 consecutive removal cycles.
研究领域:
环境科学
·
材料科学
·
生物技术
·
化学工程
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高影响因子
危险品化学品经营许可证（带存储）