ADVANCED FUNCTIONAL MATERIALS
·
2023
·Vol. 34
·期 11
·2309288
·
IF 19
Yi Liu,Mengyao Wu,Anqi Kang,Xiehaoran Zhang,Ronghui Xie,Yuanpei Huang,Jiaofang Huang,Lien-Yang Chou,Chao Zhong
Genetically modified microorganisms (GMMs) possess significant potential for a wide range of applications; however, their execution in real-world scenarios, particularly in environments characterized by scarce nutrients and unpredictable conditions, proves daunting. Drawing inspiration from the adaptability of natural seeds, living seed materials that combine a core–shell structure ZIF-8 and genetically engineered spores are introduced. By genetically modifying the Bacillus subtilis strain to express distinct fluorescent proteins, the visualization of spores within the hollow sphere is facilitated, allowing for differentiation between spore and bacteria states. Importantly, the ZIF-8 shell exhibits responsiveness to diverse nutrient levels, providing enhanced environmental resistance in comparison to bare spores upon exposure to hypochlorous acid, UV-C radiation, and even vacuum conditions. Moreover, the potential of living seed materials for living sensors of small molecules is showcased by elucidating that spores can be resuscitated into functional bacteria after release from ZIF-8 material. This research establishes a novel approach for the general fabrication of Metal-Organic Frameworks (MOF)-based living spore hybrid materials, offering environmentally controlled release of living materials across a multitude of applications.
研究领域:
生物技术
·
材料科学
·
微生物学
·
环境科学
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DOI
高影响因子
Frontiers in Microbiology
·
2017
·
IF 4.076
Tahir Hafiz A. S.,Gu Qin,Wu Huijun,Raza Waseem,Hanif Alwina,Wu Liming,Colman Massawe V.,Gao Xuewen
Bacterial volatiles play a significant role in promoting plant growth by regulating the synthesis or metabolism of phytohormones. In vitro and growth chamber experiments were conducted to investigate the effect of volatile organic compounds (VOCs) produced by the plant growth promoting rhizobacterium Bacillus subtilis strain SYST2 on hormone regulation and growth promotion in tomato plants. We observed a significant increase in plant biomass under both experimental conditions; we observed an increase in photosynthesis and in the endogenous contents of gibberellin, auxin, and cytokinin, while a decrease in ethylene levels was noted. VOCs emitted by SYST2 were identified through gas chromatography-mass spectrometry analysis. Of 11 VOCs tested in glass jars containing plants in test tubes, only two, albuterol and 1,3-propanediole, were found to promote plant growth. Furthermore, tomato plants showed differential expression of genes involved in auxin (SlIAA1. SlIAA3), gibberellin (GA20ox-1), cytokinin (SlCKX1), expansin (Exp2, Exp9. Exp 18), and ethylene (ACO1) biosynthesis or metabolism in roots and leaves in response to B. subtilis SYST2 VOCs. Our findings suggest that SYST2-derived VOCs promote plant growth by triggering growth hormone activity, and provide new insights into the mechanism of plant growth promotion by bacterial VOCs.
研究领域:
微生物学
·
植物科学
·
分子生物学
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DOI
PubMed
高影响因子
WATER SCIENCE AND TECHNOLOGY
·
2021
·Vol. 83
·期 9
·2075-2086
·
IF 1.915
Ding Jing,Chen Weiguang,Zhang Zilan,Qin Fan,Jiang Jing,He Anfei,Sheng G. Daniel
Shortcomings of individual biochar or microbial technologies often exist in heavy metal removal from wastewater and may be circumvented by coupled use of biochar and microorganisms. In this study, Bacillus subtilis and each of three biochars of different origins (corn stalk, peanut shell, and pine wood) were coupled forming composite systems to treat a cadmium (Cd, 50 mg/L) wastewater formulated with CdCl 2 in batch tests. Biochar in composite system enhanced the activity and Cd adsorption of B. subtilis . Compared with single systems with Cd removal up to 33%, the composite system with corn stalk biochar showed up to 62% Cd removal, which was greater than the sum of respective single B. subtilis and biochar systems. Further analysis showed that the removal of Cd by the corn stalk composite system could be considered to consist of three successive stages, that is, the biochar-dominant adsorption stage, the B. subtilis -dominant adsorption stage, and the final biofilm formation stage. The final stage may have provided the composite system with the ability to achieve prolonged steady removal of Cd. The biochar-microorganism composite system shows a promising application for heavy metal wastewater treatment. Biochar enhanced the activity and cadmium adsorption of Bacillus subtilis . Corn stalk composite system had higher cadmium removal efficiency than peanut shell and pine wood composite systems. Cadmiun removal in corn stalk composite system was higher than the sum of the single biochar and Bacillus subtilis systems. Cadmium removal in corn stalk composite system could be divided into three stages.
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DOI
高影响因子
Microorganisms
·
2022
·Vol. 10
·期 7
·1338
·
IF 4.926
Shanshan Xu,Qianqian Cao,Zengzhi Liu,Junpeng Chen,Peiguang Yan,Bingyu Li,Ying Xu
Bacillusstrains are widely distributed in terrestrial and marine environments, and some of them are used as biocontrol organisms for their biofilm-formation ability. InBacillus subtilis, biofilm formation is fine-tuned by a complex network, a clear understanding of which still requires study. In bacteria, tmRNA, encoded by thessrAgene, catalyzestrans-translation that can rescue ribosomes stalled on mRNA transcripts lacking a functional stop codon. tmRNA also affects physiological bioprocesses in some bacteria. In this study, we constructed assrAmutant inB. subtilisand found that the biofilm formation in thessrAmutant was largely impaired. Moreover, we isolated a biofilm-formation suppressor ofssrA, in which the biofilm formation was restored to a level even stronger than that in the wild type. We further performed RNAseq assays with the wild type,ssrAmutant, and suppressor ofssrAfor comparisons of their transcriptomes. By analyzing the transcriptomic data, we predicted the possible functions of some differentially expressed genes (DEGs) in the tmRNA regulation of biofilm formation inB. subtilis. Finally, we found that the overexpression of two DEGs,acoAandyhjR, could restore the biofilm formation in thessrAmutant, indicating that AcoA and YhjR were immediate regulators involved in the tmRNA regulatory web controlling biofilm formation inB. subtilis. Our data can improve the knowledge about the molecular network involved inBacillusbiofilm formation and provide new targets for manipulation ofBacillusbiofilms for future investigation.Keywords:tmRNA;biofilm formation;Bacillus subtilis;transcriptome;sporulation
研究领域:
微生物学
·
分子生物学
·
基因组学
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DOI
PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 3
·387
·
IF 3.06
Zhiliang Yu,Yuyi Zhu,Jianv Fu,Juanping Qiu,Jianhua Yin
The commonly believed mechanism of colistin against Gram-negative bacteria is to cause cell membrane lysis, whereas the mechanism of colistin against Gram-positive bacteria is extremely fragmented. In this study, we found that colistin treatment onBacillus subtilisWB800,Paenibacillus polymyxaC12 andPaenibacillus polymyxaATCC842 enhances not only the activities of α-ketoglutaric dehydrogenase and malate dehydrogenase in tricarboxylic acid (TCA) cycle, but also the relative expression levels of their encoding genes. Additionally, the oxaloacetate concentration also increases. Interestingly, the analysis of the relative expression of genes specific for respiratory chain showed that colistin treatment stimulates the respiratory chain in Gram-positive bacteria. Accordingly, the NAD+/NADH ratio increases and the oxidative level is then boosted up. As a result, the intensive oxidative damages are induced in Gram-positive bacteria and cells are killed. Notably, both rotenone and oligomycin, respectively, inhibiting NADH dehydrogenase and phosphorylation on respiratory chain can downgrade oxidative stress formation, thus alleviating the colistin-induced killing of Gram-positive cells. Besides, thiourea-based scavenging for reactive oxygen species also rescues the colistin-subjected cells. These data collectively demonstrate that colistin stimulates both TCA cycle and respiratory chain in Gram-positive bacteria, leading to the enhancement of NADH metabolism and resulting in the generation of oxidative damages in Gram-positive cells. Our studies provide a better understanding of antibacterial mechanism of colistin against Gram-positive bacteria, which is important for knowledge on bacterial resistance to colistin happening via the inhibition of respiratory chain and manipulation of its production.Keywords:colistin;Gram-positive bacteria;oxidative stress;TCA cycle;respiratory chain
研究领域:
微生物学
·
药理学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2023
·
IF 5.2
Sun Biao,Zou Kai,Zhao Yingqing,Tang Yinfang,Zhang Fuming,Chen Weijing,Tang Xiaoting,Chang Chenran,Zheng Yan
Introduction: Proteases exhibit a wide range of applications, and among them, alkaline proteases have become a prominent area of research due to their stability in highly alkaline environments. To optimize the production yield and activity of alkaline proteases, researchers are continuously exploring different fermentation conditions and culture medium components.Methods: In this paper, the fermentation conditions of the alkaline protease (EC 3.4.21.14) production by Bacillus subtilis BS-QR-052 were optimized, and the effect of different nutrition and fermentation conditions was investigated. Based on the single-variable experiments, the Plackett–Burman design was used to explore the significant factors, and then the optimized fermentation conditions, as well as the interaction between these factors, were evaluated by response surface methodology through the Box–Behnken design.Results and discussion: The results showed that 1.03% corn syrup powder, 0.05% MgSO4, 8.02% inoculation volume, 1:1.22 vvm airflow rate, as well as 0.5% corn starch, 0.05% MnSO4, 180 rpm agitation speed, 36°C fermentation temperature, 8.0 initial pH and 96 h incubation time were predicted to be the optimal fermentation conditions. The alkaline protease enzyme activity was estimated to be approximately 1787.91 U/mL, whereas subsequent experimental validation confirmed it reached 1780.03 U/mL, while that of 500 L scale-up fermentation reached 1798.33 U/mL. This study optimized the fermentation conditions for alkaline protease production by B. subtilis through systematic experimental design and data analysis, and the activity of the alkaline protease increased to 300.72% of its original level. The established model for predicting alkaline protease activity was validated, achieving significantly higher levels of enzymatic activity. The findings provide valuable references for further enhancing the yield and activity of alkaline protease, thereby holding substantial practical significance and economic benefits for industrial applications.
研究领域:
微生物学
·
生物化学
·
酶工程
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DOI
PubMed
高影响因子
CATALYSIS LETTERS
·
2019
·Vol. 149
·期 11
·2973-2983
·
IF 2.372
Luo Wei,Du Hui-Jun,Bonku Emmanuel Mintah,Hou Ya-Li,Li Ling-Ling,Wang Xiao-Qiang,Yang Zhong-Hua
Abstract Enantiopure alcohols have received much attention due to their widespread use as pharmaceutical intermediates. In the asymmetric biosynthesis of enantiopure alcohols, the excellent performance of carbonyl reductase makes it be the best choice as the biocatalysts. In this work, an alkali-tolerant carbonyl reductase ( Bs CR, encoded by yueD ) from Bacillus subtilis (strain 168) was obtained through gene mining, and successfully heterologously expressed in Escherichia coli with pET-32a. Bs CR showed excellent alkali resistance and even can keep more than 70% of its peak activity after incubation in Tris–HCl buffer at pH 9.0 for 40 h. The Michaelis constants and maximal velocity of the Bs CR to NADPH (A) and ethyl 4-chloroacetoacetate (B) are \(K_{m}^{A}\) = 5.390 × 10 −2 mmol/L, \(K_{m}^{B}\) = 1.855 mmol/L, and \(V_{max}\) = 147.3 μmol·min −1 ·mg −1 , respectively. Applying the E. coli BL21(DE3)/pET-32a-yueD to catalyze asymmetric reduction of ethyl 4-chloroacetoacetate and acetophenone, the yield of S -CHBE reached 89.9% and S -1-phenyl ethanol reached 66.7%, and e.e. of both products reached more than 99%. This work provides a novel CR for asymmetric reduction. Graphic Abstract A carbonyl reductase ( Bs CR) and its gene were identified through gene mining, and overexpressed in Escherichia coli BL21(DE3) for whole-cell biocatalytic asymmetric reduction
研究领域:
生物技术
·
生物化学
·
药物科学
·
酶学
✓ 已验证
DOI
高影响因子
Journal of Biobased Materials and Bioenergy
·
2017
·Vol. 11
·期 3
·210-215
·
IF 0.975
Wang Qing-Yun,Lin Qin-Lu,Peng Kuan,Cao Jian-Zhong,Yang Chun,Xu Dong
An antifungal Bacillus strain isolated from the traditional fermented food natto was identified and named as B. subtilis natto CSUF5. Antifungal metabolites of the strain were extracted from the medium of the inhibition zone on the dual culture plate. 2 V7-surfactin
isoforms (C14–V7, C13–Emet
1-V7) and 3 I/L7-surfactin isoforms (C13–I/L7, C13,14–Emet
1–I/L7 were identified from the RP-HPLC
isolations by MS
n
(n = 1,2,3) analysis. Both V7-surfactins and I/L7 -surfactins showed slight antifungal activity against A. niger, and their MIC50 decreases in the order V7 > I/L
7. EDTA was found
to be an effective strengthening agent which could cooperate with the surfactin variants and enhance their antifungal activity greatly. Microscopic observations revealed that the surfactin variants could delay the fungal spore germination and inhibit the hyphae growth, mainly displayed in
hyphal shriveling and distortion. Surfactin variants and EDTA together could lead the hyphae to severe helical warping and fracture. These results will provide promising references for the development of new antifungal products.
研究领域:
微生物学
·
应用微生物学
·
天然产物化学
·
抗真菌剂
·
发酵食品
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DOI
高影响因子
Journal of Environmental Sciences
·
2010
·
IF 1.412
Shuyan Zang,Bin Lian,Juan Wang,Yanjie Yang
To explore biodegradation of 2-naphthol and its metabolites accumulated in wastewater treatment, a series of bio-degradation experiments were conducted. Two main metabolites of 2-naphthol, 1,2-naphthalene-diol and 1,2-naphthoquinone, were identified by high-performance liquid chromatography with standards. Combining fungus Aspergillus niger with bacterium Bacillus subtilis in the treatment enhanced 2-naphthol degradation efficiency, lowered the accumulation of the two toxic metabolites. There were two main phases during the degradation process by the kinetic analysis: 2-naphthol was first partly degraded by the fungus, producing labile and easily accumulated metabolites, and then the metabolites were mainly degraded by the bacterium, attested by the degradation processes of 1,2-naphthalene-diol and 1,2-naphthoquinone as sole source of carbon and energy. Sodium succinate, as a co-metabolic substrate, was the most suitable compound for the continuous degradation. The optimum concentration of 2-naphthol was 50 mg/L. The overall 2-naphthol degradation rate was 92%, and the COD Cr removal rate was 80% on day 10. These results indicated that high degradation rate of 2-naphthol should not be considered as the sole desirable criterion for the bioremediation of 2-naphthol-contaminated soils/wastewater.
研究领域:
环境科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
ANNALS OF MICROBIOLOGY
·
2020
·Vol. 70
·期 1
·1-10
·
IF 1.528
Xie Shanshan,Liu Juan,Gu Shuangyue,Chen Xiaojie,Jiang Haiyang,Ding Ting
PurposeTo screen endophytic Bacillus producing volatile organic compounds (VOCs) with antifungal activity, and to explore their biocontrol properties toward the growth and pathogenicity of Curvularia lunata.Methods Two-sealed-base-plate assays were used to estimate the antifungal activities of Bacillus strains against C. lunata. Conjoint analysis of solid-phase microextraction gas chromatography-mass spectrometry and antagonistic experiments were used to identify the VOCs responsible for the antifungal activity. Effects of individual synthetic VOCs were analyzed along with reactive oxygen species (ROS) accumulation in C. lunata conidia. After exposure to individual VOCs, conidia were also sprayed onto maize leaves to evaluate their pathogenicity. Expression levels of virulence-related genes in C. lunata mycelium following exposure to VOCs were analyzed using quantitative real-time PCR.Results Among the ten endophytic Bacillus strains and two plant growth-promoting rhizobacterial (PGPR) strains, only B. subtilis strain DZSY21 strongly inhibited the growth of C. lunata by producing VOCs. 2-Methylbutyric acid, 2-heptanone, and isopentyl acetate produced by strain DZSY21 showed inhibitory effects on the mycelia growth and conidial sporulation of C. lunata. 2-Heptanone and isopentyl acetate also repressed the germination of conidia and the expression levels of virulence-related genes in C. lunata mycelium. Moreover, isopentyl acetate strongly enhanced the accumulation of intracellular ROS in conidia. The disease indexes of maize leaves sprayed with VOC-treated C. lunata conidia were reduced from 60.52 to 26.64%.Conclusion Endophytic B. subtilis strain DZSY21 displayed the potential to control C. lunata by producing VOCs, especially 2-heptanone and isopentyl acetate.
研究领域:
植物病理学
·
微生物学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Bingfeng Li,Siyuan Chang,Di Jin,Sen Zhang,Tianyi Chen,Xin Pan,Bo Fan,Kemin Lv,Xuejun He
A phenolic-UDP-glycosyltransferase Bs-PUGT from Bacillus subtilis PI18 was cloned and expressed in Escherichia coli BL21 (DE3). The purified Bs-PUGT could catalyze the glycosylation of tyrosol, 4-hydroxybenzyl alcohol, 2-hydroxybenzyl alcohol, caffeic acid, cinnamic alcohol, ferulic acid, and so on. This enzyme showed a high activity and stability over a broad pH range and was sensitive to temperature. Studies on the kinetic parameters indicated that the affinity of Bs-PUGT to UDP-G ( K m) and its catalytic efficiency ( K cat) increased by 1.5-fold and 1.7-fold, respectively, with the addition of 10 mM Ca 2+ . The most effective glycosylation of caffeic acid catalyzed by whole-cell E. coli /Bs-PUGT was achieved with a molar yield of 78.3% in a system with pH 8.0, 30 °C, 25 g/L sucrose, 10 mM Ca 2+ , and 0.5 g/L substrate concentration. The addition of Ca 2+ increased the molar yield of caffeic acid glucosides and shortened the reaction. This work proposes a strategy for the efficient glycosylation of phenolic compounds by microbe-derived glycosyltransferase assisted by metal ions.
研究领域:
生物化学
·
生物技术
·
酶学
✓ 已验证
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2018
·
IF 4.61
Guo-Qiang Li,Zheng-Yang Huo,Qian-Yuan Wu,Yun Lu,Hong-Ying Hu
An ultraviolet (UV)-based advanced oxidation process (AOP) for disinfecting water is introduced in this paper. This study aimed to evaluate the potential of UV light-emitting diodes (UV-LEDs)/chlorine AOP (UV/Cl 2 ) for Bacillus subtilis spore inactivation. Chlorine was combined with 265 and 280 nm LEDs (UV 265 /Cl 2 , UV 280 /Cl 2 ) and investigated. The addition of 4.0 mg/L of free chlorine at pH 7.0 in the presence of 125 mJ/cm 2 of UV irradiation resulted in an additional 1.8-log reduction in UV 265 /Cl 2 and 1.5-log reduction in UV 280 /Cl 2 . There was no observed enhancement in spore inactivation with the addition of a radical scavenger, t-BuOH, which indicated the role of • OH in the synergistic effect. To quantitatively evaluate the synergism, the primary treatment with UV/Cl 2 was followed by further UV or Cl 2 treatment. After UV/Cl 2 pretreatment at different pH levels, the 265 and 280 nm LEDs treatment enhanced an approximate 0.4–0.5-log reduction compared to UV only, and Cl 2 treatment enhanced an approximate 0.7–1.1-log reduction compared to Cl 2 only. In addition, at pH 7.0, in UV 265 /Cl 2 -Cl 2 and UV 280 /Cl 2 -Cl 2 , the inactivation rate constant k increased by approximately 2 and 1.5 times, respectively. The CT for the lag phases ( CT lag ) reduced to approximately 67 and 58%, respectively. Similar results were obtained at pH 7.5 and 8.0, and in the secondary effluent. The synergistic effect on spore inactivation suggested that the pathogen inactivation efficiency of sequential UV and chlorine disinfection processes, which are commonly applied, can be significantly enhanced by adding chlorine prior to UV treatment .
研究领域:
微生物学
·
环境科学
·
水处理技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）