BIOSENSORS & BIOELECTRONICS
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2013
·
IF 5.437
Ruimin Li,Mingming Zhen,Mirong Guan,Daiqin Chen,Guoqiang Zhang,Jiechao Ge,Ping Gong,Chunru Wang,Chunying Shu
C 60 -carboxyfullerene, C 60 [C(COOH) 2 ] 2 , has been proven to function as peroxidase mimetics that can catalyze the reaction of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H 2 O 2 to produce a blue color reaction. Kinetic studies further indicate that C 60 [C(COOH) 2 ] 2 has an even higher affinity to TMB than that of the natural enzyme , horseradish peroxidase (HRP). C 60 [C(COOH) 2 ] 2 /glucose oxidase (GO x )/TMB system provides a novel colorimetric sensor for glucose and shows good response toward glucose detection over a range of 1.0–40 μM with a limit of detection 0.5 μM (3 σ /slope). Moreover, this sensitive and selective sensor can be successfully applied for the quantitative determination of glucose in human serum. The results indicate that it is a simple, cheap, convenient, highly selective, sensitive, and easy handling colorimetric assay .
研究领域:
生物化学
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医学检测
·
纳米材料
✓ 已验证
DOI
高影响因子
Nanoscale
·
2017
·Vol. 9
·期 25
·8825-8833
·
IF 7.367
Zhen Hu,Cheng Wang,Feng Zhao,Xirong Xu,Shuhong Wang,Long Yu,Dayu Zhang,Yudong Huang
The wonderful chemical structures and characteristics of low-dimensional carbon materials have exciting applications in life sciences. In the present study, we developed a facile strategy to conjugate C60 with graphene via host–guest chemistry for targeted phototherapy. A versatile carrier based on folic acid-functionalized graphene (GO-FA) and comprising γ-cyclodextrin (γ-CD) at its surface was assembled via π–π interaction, creating hybrid structures with drug storage and tumor targeting properties. This γ-CD-modified graphene (GO-FA/Py-γ-CD) is capable of hosting pristine C60 molecules for the fabrication of a GO-FA/Py-γ-CD/C60 nanohybrid. The hybridization of GO-FA, γ-CD, and C60 hinders the aggregation of C60, promotes cellular uptake, enhances light absorption, and finally demonstrates enhanced phototherapy effects of GO-FA/Py-γ-CD/C60. Under Xe lamp irradiation (2 W cm−2) for 4 min, GO-FA/Py-γ-CD/C60 simultaneously causes heating and intracellular ROS production, which further significantly reduces the cell viability to 16.2% at low content of loading (30 μg mL−1). Moreover, it represents an excellent tumor killing efficiency, better than that of the other reported graphene/C60 nanohybrids; thus, this material is suitable for applications in phototherapy of cancer.
研究领域:
生物医学工程
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纳米医学
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肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 166
·期 2
·B49
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IF 3.12
Di Liu,Fangxin Hu,Han Zhang,Cong Zhang,Shihong Chen
A sandwich electrochemiluminescence (ECL) biosensing system for soybean agglutinin (SBA) detection was developed with AuPt@C60 nanoflowers (AuPt@C60 NFs) as a novel co-reactant accelerator to enhance the performance of N-(aminobutyl)-N-(ethylisoluminol) (ABEI). In this strategy, nanoflower-liked AuPt@C60 complex served as a matrix for loading a large sum of ABEI and galactosamine (Gal) by Au-N and Pt-N bonds to achieve the nanocomposite Gal-ABEI-AuPt@C60 NFs. Dissolved oxygen was used as a co-reaction reagent, while AuPt@C60 complex acted as a co-reactant accelerator for dissolved oxygen to enhanced the ECL emission of ABEI. Gal, as an identifying element, was highly loaded by electrodeposited gold nanoparticles (Dp Au) on the electrode to recognize SBA, and further combine with Gal-ABEI-AuPt@C60 NFs to form a sandwich model. As a result, the proposed SBA biosensor exhibited a wide linear range from 1.0 ng·mL−1 to 5.0 μg·mL−1 and a low detection limit of 0.33 ng·mL−1. In addition, the biosensor displayed an excellent selectivity and an acceptable stability and reproducibility. The combination of AuPt@C60 NFs and ABEI provided a new ECL sensing platform without exogenous co-reactant.
研究领域:
生物传感器
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分析化学
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食品科学
✓ 已验证
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 22
·18102-18108
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IF 3.201
Yihan Ma,Xiaoyan Zhang,Yinjia Cheng,Xiaosui Chen,Yong Li,Aiqing Zhang
Designing and constructing favorable water-dispersible fullerenes and their derivatives are of huge importance for biological applications addressing DNA-cleavage and photodynamic therapy (PDT). In the present work, a mild, green and facile synthetic approach for the preparation of C60 nanoparticles was developed for the first time via the combination of mussel-inspired chemistry and the Michael addition reaction. The resultant C60–PDA–PEI nanoparticles were characterized by transmission electron microscopy (TEM), dynamic laser scattering (DLS), Fourier-transform infrared spectroscopy (FT-IR), Raman spectra, X-ray photoelectron spectra (XPS) and thermogravimetric analysis (TGA), demonstrating that the above two-step strategy allows easy access to the preparation of highly water-dispersible fullerene derivatives. Benefiting from their unique nanostructure, the versatile C60–PDA–PEI nanoparticles display a uniform hydrodynamic size of 160 nm in water and efficient 1O2 generation under irradiation. Furthermore, the good ability of cleaving DNA under visible light at a mass concentration of 62.5 ng μL−1 gives them high potential as PDT agents. The universal approach described in this work is capable of introducing many other functional molecules onto PDA-modified fullerenes, thus extending the possible applications of fullerene-based species in many fields of biotechnology and pharmaceutical chemistry.
研究领域:
生物技术
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药学
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纳米医学
✓ 已验证
产品关联
DOI
高影响因子
Energy Materials
·
2023
·Vol. 3
·期 1
·N
·
IF 0
Xin Liang,Dongqing Zhao,Lulu Wang,Qianqian Huang,Chonghai Deng,Lili Wang,Lei Hu,Sheng Liang,Huaxia Deng,Hongfa Xiang
A gelatin-based nanofiber separator doped with C60 and ZIF-67 was constructed by electrostatic spinning of two different solutions. It can be used as a multifunctional separator for Li-S batteries.
研究领域:
材料科学
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电化学
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纳米技术
·
能源存储
·
高分子材料
✓ 已验证
DOI
TALANTA
·
2020
·
IF 5.339
Zhang-Na Qin,Qiong-Wei Yu,Ping Zhou,Yu-Qi Feng
Bioactive polyamines play important roles in many biological processes such as gene expression, cell growth, protein synthesis , and signal transduction . Accurate determination of polyamines is helpful for studying their biological functions. Herein, a C 60 -based chemical labeling strategy was proposed for the determination of polyamines (putrescine, cadaverine , spermidine , and spermine) in biological samples using matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). An N-hydroxysuccinimide ester functionalized C 60 (NHS–C 60 ) was used as a labeling reagent and the m/z of the labeled polyamines reached up to more than 900 Da, which avoided matrix interferences in the low m/z region. In addition, as NHS–C 60 derivatives, mono- and bis-substituted polyamines were produced simultaneously, which benefited the qualitative analysis of polyamines. The analytical method was validated using NHS–C 60 labeled polyamines in cells and mice feces samples. Good linearities were obtained with correlation coefficients ranging from 0.9786 to 0.9982. The limits of quantification were in the range of 0.68–1.48 pmol. Good reproducibility and reliability of our proposed method were confirmed by intra- and inter-day precisions ranged from 2.8 to 16.6%, and the recoveries ranged between 81.8 and 119.9%. Finally, the proposed method was applied to determine polyamines in cells and mice feces. Three polyamines were detected in the cells, and the contents of cadaverine and spermidine in the feces of high-fat diet mice were found to be significantly lower than those in the normal diet mice. The results show that the proposed NHS–C 60 labeling coupled with MALDI MS strategy is suitable for the determination of polyamines in biological samples.
研究领域:
分析化学
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生物化学
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医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
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2018
·Vol. 8
·期 33
·18197-18203
·
IF 2.936
Minglei Chu,Huichang Gao,Sa Liu,Lin Wang,Yongguang Jia,Meng Gao,Miaojian Wan,Chengfang Xu,Li Ren
A series of novel bacterial cellulose/C60 (BCC60) composites was prepared using a original dehydration-rehydration method. The composites were characterized to demonstrate their potential in multifunctional wound dressings for skin cancer treatment using photodynamic therapy. Raman spectroscopy revealed that the C60 nanoparticles were successfully incorporated into the bacterial cellulose (BC) network. Scanning electron microscopy was used to examine the morphology and distribution of the C60 particles as photosensitizers in the bacterial cellulose network, and the C60 particles were uniformly distributed in the hyperfine three-dimensional BC network with diameters less than 100 nm. Reactive oxygen species (ROS) measurements indicated that the BCC60 composites possessed a high ROS generation ability when exposed to light. The antibacterial assessment of the BCC60 composites revealed their ability to inhibit the growth of E. coli and S. aureus and their relationship with light irradiation. In vitro cell experiments also confirmed that the BCC60 composites had low cytotoxicity in the dark, while they exhibited significant cancer cell damage activity under visible light.
研究领域:
生物材料学
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医学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Energy Materials
·
2019
·Vol. 10
·期 6
·1903292
·
IF 24.884
Qingshuai Xu,Jiajin Lin,Changchun Ye,Xiaojing Jin,Daiqi Ye,Yingying Lu,Guangmin Zhou,Yongcai Qiu,Weishan Li
Li metal is an ideal anode material for rechargeable high energy density batteries, but its sensitivity to humid air and uncontrolled dendrite growth limit its practical applications. A novel hybrid interphase is fabricated to address these issues. This interphase consists of dense fullerene (C 60 ) and magnesium metal bilayers, which are deposited successively on lithium foil by vacuum evaporation deposition and contribute to moisture resistance and lithium dendrite suppression. Thanks to this dual-functional feature, the assembled cells with the modified anodes and commercial LiFePO 4 cathodes exhibit long cycle life (>200 cycles) with high capacity retention (>98.5%). Moreover, even the modified anodes that are exposed to humid air (30% relative humidity) for over 12 h; the cells still deliver excellent performance, comparable to those without exposure. Such a unique hybrid interphase provides a new promising method for fabricating air-stable and dendrite-free lithium metal batteries.
研究领域:
材料科学
·
电化学
·
能源存储
✓ 已验证
DOI
高影响因子
Science China-Chemistry
·
2017
·Vol. 60
·期 4
·490-496
·
IF 4.132
Li Qinfen,Wu Jiake,Wu Ruihan,Liu Yujing,Chen Hongzheng,Huang Fei,Li Hanying
Contact resistance at the interface between metal electrodes and semiconductors can significantly limit the performance of organic field-effect transistors, leading to a distinct voltage drop at the interface. Here, we demonstrate enhanced performance of n-channel field-effect transistors based on solution-grown C 60 single-crystalline ribbons by introducing an interlayer of a conjugated polyelectrolyte (CPE) composed of poly[(9,9-bis(3′-(( N , N -dimethyl)- N -ethylammonium)-propyl)-2,7-fluorene)-alt-2,7-(9,9-dioctylfluorene)] dibromide (PFN + Br − ). The PFN + Br − interlayer greatly improves the charge injection. Consequently, the electron mobility is promoted up to 5.60 cm 2 V −1 s −1 and the threshold voltage decreased dramatically with the minimum of 4.90 V.
研究领域:
材料科学
·
电子工程
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Zenghui Liu,Hesen Wang,Jinge Li,Mengli Wang,Huaixia Yang,Fuchun Si,Jinming Kong
As a new serum biomarker, the sensitive detection of exosomes is significant for the early diagnosis of diseases. In this paper, a novel electrochemical biosensor for the sensitive detection of exosomes was constructed utilizing a novel C 60 hybrid as a tracer. Firstly, the epidermal growth factor receptor (EGFR) antibody was immobilized on the electrode surface via Au-NH 2 bonding with Nafion AuNPs substrate. After specific capture of exosomes, C 60 -Au-Tb was grafted onto the surface of exosomes through “carboxyl-Zr 4+ -phosphate” chemistry, causing a change in the current signal. Under the optimal conditions, there was a linear relationship between the SWV signal and the logarithm of exosomes concentration from 5 × 10 4 exosomes/mL to 5 × 10 9 exosomes/mL, and the detection limit was 2.67 × 10 4 exosomes/mL. In addition, the biosensor had the advantages of high specificity, strong anti-interference ability, good repeatability, and stability. In conclusion, this method had great potential in diagnosing early cancers associated with exosomes.
研究领域:
生物传感器
·
分析化学
·
癌症诊断
✓ 已验证
产品关联
DOI
高影响因子
SURFACE & COATINGS TECHNOLOGY
·
2023
·
IF 5.4
Yuan Gao,Chen Shu,Shengliang Zhao,Wei Wang
Poly-ether-ether-ketone (PEEK) has many excellent properties for being used as self-lubricating coatings. In order to further improve the friction performance, black phosphorus (BP) was used as filler to prepare BP/PEEK composite coating . BP-C 60 was formed by modification of BP edge by fullerene C 60 to solve the problem of BP oxidation , so that BP still existed in the sintered PEEK coating. Then, the effects of C 60 and BP-C 60 on the tribological properties of PEEK coating were studied, and the results showed that the wear rate and friction coefficient of BP-C 60 /C 60 /PEEK composite coating were significantly reduced. This was attributed to the synergistic effect of BP and C 60 , which enhanced the stability of BP and reduced coating porosity.
研究领域:
材料科学
·
摩擦学
✓ 已验证
产品关联
DOI
高影响因子
Results in Physics
·
2020
·
IF 4.019
Yuan Zhao,Xiaoliang Liu,Guangdi Feng,Lu Lyu,Lin Li,Shitan Wang,Jie Jiang,Youzhen Li,Dongmei Niu,Haipeng Xie,Yongli Gao
The modification of C 60 nano-interlayers on the organic field-effect transistors (OFETs) based on 2,7-diocty[1]benzothieno-[3,2-b]benzothiophene (C8-BTBT)/SiO 2 was addressed at both the electronic structure and device level. It is observed that a band bending occurs in C8-BTBT layer with the insertion of C 60 , which induces a strong hole accumulation at the interface region, facilitating the formation of high concentration conducting channel when the interface is incorporated into OFETs. The strong acceptor C 60 may cause charge transfer from the trap states in C8-BTBT, resulting in a field-effect operation with decreased threshold voltage. However, the increasing disorder in C8-BTBT induced by the C 60 nano-interlayer may reduce the hole mobility at the C8-BTBT/SiO 2 interface region and change the growth model of C8-BTBT. In addition, as confirmed by the transfer characteristic of the OFETs, the thin C 60 insertion layer isn’t continuous enough to induce an ambipolar transport behavior despite its high mobility. The competition between the two opposite effects results in compromised performance in such OFETs. The device performance parameters deteriorate generally with increasing the C 60 nano-interlayer thickness. So, it is inferred that the C 60 interlayer alone cannot improve the performance of the C8-BTBT/SiO 2 -based OFETs without further optimization. Importantly, the device performance exhibits an abnormal improvement with a 6 nm C 60 interlayer, which might be ascribed to a higher energy level shift of C8-BTBT caused by a quasi-continuous C 60 film at this thickness.
研究领域:
材料科学
·
电子工程
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）