FOOD CHEMISTRY
·
2025
·
IF 9.8
Youfeng Zhang,Zhimian Liu,Jiaheng Li,Zhuoneng Huang,Chen Chang Chiam,Jialing Gu,Yujie Su,Xiaosan Wang
A human milk fat substitute (HMFS) based on Carassius auratus oil (CAO) and plant oils was developed. The resulting HMFS mimicked the lipid composition and structural characteristics of human milk fat, potentially promoting infant digestion and absorption. The optimal conditions to enrich C52 (52 carbon atoms) triacylglycerols (TAGs) through solvent fractionation were a mixing ratio of CAO to acetone of 1:8 ( w / v ) and extraction at −40 °C for 24 h. The C52 TAGs content increased to 61.30 %, a 20.38 % improvement compared to unfractionated CAO. The total palmitic acid increased to 24.83 %, with 47.27 % located at sn -2. The in vitro digestion revealed a maximum free fatty acid release rate of 60.94 % from the CAO HMFS emulsion, with oleic acid (44.23 %) and linoleic acid (22.71 %) as the main components. Palmitic acid monoacylglycerol accounted for 72.16 % of the digestion products, suggesting a favorable TAG structure and potential benefits for infant lipid absorption.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Metabolites
·
2023
·Vol. 13
·期 2
·159
·
IF 4.1
Lu Ding,Yingjie Liu,Xiaofeng Wei,Chuanye Geng,Wenzhi Liu,Lin Han,Fangying Yuan,Peng Wang,Yanchun Sun
The salinization of the water environment caused by human activities and global warming has increased which has brought great survival challenges to aquatic animals. Crucian carp (Carassius auratus) is an essential freshwater economic fish with superior adaptability to saline-alkali water. However, the physiological regulation mechanism of crucian carp adapting to saline-alkali stress remains still unclear. In this study, crucian carp were exposed to freshwater or 20, 40, and 60 mmol/L NaHCO3water environments for 30 days, the effects of saline-alkali stress on the kidney were evaluated by histopathology, biochemical assays and metabolomics analysis from renal function, antioxidant capacity and metabolites level. Our results showed different degrees of kidney damage at different exposure concentrations, which were characterized by glomerular atrophy and swelling, renal tubular degranulation, obstruction and degeneration, renal interstitial edema, renal cell proliferation and necrosis. Saline-alkali stress could change the levels of several physiological parameters with renal function and antioxidant capacity, including creatinine (CREA), urea nitrogen (BUN), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA). In addition, metabolomics analysis showed that differential metabolites (DMs) were involved in various metabolic pathways, including phenylalanine, tyrosine, and tryptophan biosynthesis, aminoacyl-tRNA biosynthesis, purine metabolism, glycerophospholipid metabolism, sphingolipid metabolism, glycolysis/gluconeogenesis and the TCA cycle. In general, our study revealed that saline-alkaline stress could cause significant changes in renal function and metabolic profiles, and induce severe damage in the crucian carp kidney through destroying the anti-oxidant system and energy homeostasis, inhibiting protein and amino acid catabolism, as well as disordering purine metabolism and lipid metabolism. This study could contribute to a deeper understanding the adverse effects of saline-alkali stress on crucian carp kidney and the regulatory mechanism in the crucian carp of saline-alkali adaptation at the metabolic level.Keywords:saline-alkaline stress;crucian carp;kidney;metabolomics;histopathology
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2013
·
IF 2.203
R.-J. Qu,X.-H. Wang,M.-B. Feng,Y. Li,H.-X. Liu,L.-S. Wang,Z.-Y. Wang
This study investigated the effect of pH on cadmium toxicity to three aquatic organisms: Photobacterium phosphoreum , Daphnia magna and Carassius auratus . The acute toxicity of Cd 2+ to P. phosphoreum and D. magna at five pH values (5.0, 6.0, 7.0, 8.0, and 9.0) was assessed by calculating EC 50 values. We determined that Cd 2+ was least toxic under acidic conditions, and D. magna was more sensitive to the toxicity of Cd than P. phosphoreum . To evaluate Cd 2+ -induced hepatic oxidative stress in C. auratus at three pH levels (5.0, 7.25, 9.0), the activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase), the level of glutathione and the malondialdehyde content in the liver were measured. Oxidative damage was observed after 7 d Cd exposure at pH 9.0. An important finding of the current research was that Cd 2+ was generally more toxic to the three test organisms in alkaline environments than in acidic environments.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2018
·
IF 3.185
Qijun Le,Jiabao Hu,Xiaohuan Cao,Siwen Kuang,Man Zhang,Na Yu,Huakun Zheng,Yajun Wang,Hanwei Liu,Xiaojun Yan
Stress response has negative effect on fish in aquaculture and research, which can be alleviated with anesthetic. To determine the optimal anesthetic, we investigated the physiological response of crucian carp ( Carassius auratus ) treated with three different anti-stress treatments: MS-222, eugenol and percussive stunning. Stress responses were evaluated by analyzing serum cortisol level and gene expression in blood. We determined the optimal concentrations of MS-222 (100 mg L −1 ) and eugenol (20 mg L −1 ) by dose selection. We found that the control group had significantly higher cortisol levels (172.78 ± 19.95 ng mL −1 ) compared to the MS-222 treated group (46.85 ± 3.22 ng mL −1 ), the eugenol treated group (72.78 ± 9.07 ng mL −1 ), and the stunning treatment group (82.78 ± 8.16 ng mL −1 ). Transcriptome analysis revealed 1572 differentially expressed genes (DEGs), including 155 DEGs related to the stress response, mainly involved in oxidative-stress response, heat shock proteins , and cold shock domain-containing protein. The heat shock protein genes were the primary DEGs in response to stress. RT-qPCR analysis confirmed differential expression of Hsps. We analyzed the function of the DEGs, which were enriched in genes involved in cellular response to stress and antigen processing and presentation. Combining the results from biochemical, transcriptome, and gene expression analysis, our data suggest that eugenol is more effective than MS-222 and percussive stunning in alleviating stress in crucian carp.
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENT INTERNATIONAL
·
2022
·
IF 13.352
Peng Zhang,Guanghua Lu,Yu Sun,Jiaqi Zhang,Jianchao Liu,Zhenhua Yan
Size effects of microplastics have received extensive attention for their influence on other pollutants and harm to organisms. In this study, we investigated the uptake, elimination, tissue distribution and potential toxicity mechanism of roxithromycin (ROX) in the presence of 0.5, 5 and 50 μm of aged microplastics (AMPs) in Carassius auratus . The results showed that AMPs promoted the ROX bioaccumulation of various tissues in a size-dependent manner. AMPs and ROX significantly induced superoxide dismutase and catalase activities of liver and gut, and inhibited acetylcholinesterase activities of brain. The coexistence of smaller AMPs exacerbated pathological abnormalities in liver, gill and brain induced by ROX, while larger AMPs caused more intestinal damage. Moreover, high-throughput 16S rRNA gene sequencing indicated that the abundance of Proteobacteria in 0.5 μm AMPs and ROX joint treatments and Firmicutes and Bacteroidota in 50 μm AMPs and ROX joint treatments were significantly raised ( p < 0.05). Metabolomics revealed that AMPs and ROX had a size-dependent long-term effect on gut microbial metabolites, which was mainly related to galactose metabolism, amino acid metabolism and primary bile acid biosynthesis pathways after a 7-day elimination, respectively. These results provide important insights into the relationship between the size effect of AMPs and interaction mechanism of AMPs and coexisting pollutants on aquatic organisms.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2022
·
IF 4.52
Zhuoneng Huang,Keying Liu,Ye Chen,Ye Huang,Xiaohan Wang,Xiaosan Wang
Most infant formula oils differ significantly from human milk fat (HMF) in structure and composition , which is detrimental to the development of infants. In this study, the lipid compositions of Carassius auratus oils (CAOs) from seven provinces of China were systematically analyzed, and the potential of CAO for use in human milk fat substitutes (HMFS) was evaluated. First, the main fatty acids in most CAOs were confirmed to be palmitic, oleic and linoleic acids . It is interesting to find that 57.08–69.27% of palmitic acid tended to be located at the sn -2 position, which showed a consistency with HMF. Afterwards, content of C52 triacylglycerol in CAOs from three regions exceeded 45%. Finally, a HMFS containing CAO from Jiangsu province was prepared by physical blending and it was verified to have a high similarity with HMF after similarity evaluation, which can provide a reference for the application of CAO in infant formulas.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Peng Zhang,Guanghua Lu,Yu Sun,Zhenhua Yan,Tianjian Dang,Jianchao Liu
The aging process changes the physicochemical structure of microplastics and affects environmental behaviors and toxicological effects of coexisting pollutants, thereby posing ecological risks. In this study, the effects of aged polystyrene microplastics alone or in combination with the 100 μg/L roxithromycin (ROX) on intestines of Carassius auratus were investigated. The carrier effect of microplastics was enhanced by aging due to changes in functional groups and surface area, which led to an increase in the bioaccumulation of ROX. The combined exposure of aged microplastics (APS) and ROX caused more inflammatory cell infiltration and cilia defects, and significantly inhibited the activity of amylase and lipase. Metagenomic sequencing revealed that the combined exposure of microplastics and ROX increased the abundance of Gemmobacter , Bosea , Rhizobium , and Shinella and decreased the abundance of Cetobacterium and Akkermansia ( p < 0.05). The presence of APS enhanced the selective enrichment of antibiotic resistance genes. What's more, the influence of microplastics and antibiotics on gut microbiota was closely related to carbohydrate metabolism and amino acid metabolism activities, as well as the abundance of baca and sul1 resistance genes. These results expand our understanding of the interaction mechanism between APS and antibiotics in real aquatic environment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·
IF 5.8
Meng Hui-Hui,Liu Wen-Ya,Zhao Wen-Long,Zheng Qi,Wang Jun-Song
Trichlorfon, one of the most widely used organophosphate insecticides, is commonly employed in aquaculture and agriculture to combat parasitic infestations. However, its inherent instability leads to rapid decomposition into dichlorvos (DDVP), increasing its toxicity by eightfold. Therefore, the environmental effects of trichlorfon in real-world scenarios involve the combined effects of trichlorfon and its degradation product, DDVP. In this study, we systematically investigated the degradation of trichlorfon in tap water over time using HPLC and LC–MS/MS analysis. Subsequently, an experiment was conducted to assess the acute toxicity of trichlorfon and DDVP on goldfish ( Carassius auratus ), employing a 1 H NMR-based metabolic approach in conjunction with serum biochemistry, histopathological inspection, and correlation network analysis. Exposure to trichlorfon and its degradation product DDVP leads to increased lipid peroxidation, reduced antioxidant activity, and severe hepatotoxicity and nephrotoxicity in goldfish. Based on the observed pathological changes and metabolite alterations, short-term exposure to trichlorfon significantly affected the liver and kidney functions of goldfish, while exerting minimal influence on the brain, potentially due to the presence of the blood–brain barrier. The changes in the metabolic profile indicated that trichlorfon and DDVP influenced several pathways, including oxidative stress, protein synthesis, energy metabolism, and nucleic acid metabolism. This study demonstrated the applicability and potential of 1 H NMR-based metabonomics in pesticide environmental risk assessment, providing a feasible method for the comprehensive study of pesticide toxicity in water environments. Graphical abstract
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Di Cui,Peng Zhang,Haipu Li,Zhaoxue Zhang,Wenbao Luo,Zhaoguang Yang
The metabolic process and toxicity mechanism of dietary inorganic arsenic (iAs) in freshwater fish remain unclear to date. The present study conducted two iAs [arsenate (As(V)) and arsenite (As(III))] dietary exposures in freshwater fish crucian carp ( Carassius auratus ). The fish were fed on As supplemented artificial diets at nominal concentrations of 50 and 100 μg As(III) or As(V) g −1 (dry weight) for 10 d and 20 d. We found that the liver, kidney, spleen, and intestine of fish accumulated more As in As(V) feeding group than that in As(III), while the total As levels in muscle were similar between As(V) and As(III) group at the end of exposure. Reduction of As(V) to As(III) and oxidation of As(III) to As(V) occurred in fish fed with As(V) and As(III), respectively, indicating that toxicity of iAs was likely elevated or reduced when iAs was absorbed by fish before entering into human body through diet. Biomethylation to monomethylarsonic acid and dimethylarsinic acid and transformation to arsenocholine and arsenobetaine were also found in the fish. The linear regression analysis showed a positive correlation between secondary methylation index and the malondialdehyde content in tissues, highlighting the vital role of arsenic dimethylation in the oxidative damages in fish.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Keheng Zhu,Zhenhua Zhu,Shanshan Xu,Cheng Zhao,Tianjun Ni
Trimethylamine (TMA) is an organic amine with strong pungent smell which is an indicator gas for evaluating fish freshness according to the international standard. In this work, as-synthesize α-Fe 2 O 3 solid nanocubes (NCs), α-Fe 2 O 3 nucleoshell NCs and α-Fe 2 O 3 hollow NCs were used as sensing material to develop an outstanding TMA gas sensor. The response of the α-Fe 2 O 3 hollow NCs sensors towards 20 ppm TMA at 230 ℃ was 6.3. Meanwhile, these sensors showed exceptional response/recovery time, low limit of detection, great selectivity, and outstanding linear relationship. Furthermore, the analysis of gases released during the decomposition of Carassius auratus (0–10 days) was conducted, which demonstrated the assessment of TMA by α-Fe 2 O 3 hollow NCs sensor can evaluate the freshness of Carassius auratus . Such a novel sensor signifies the outstanding application potential in efficient gas-sensing properties of TMA, which will make the tremendous contribution for Carassius auratus product evaluation in the future.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2018
·Vol. 25
·期 10
·9636-9646
·
IF 2.8
Li Chenguang,Qu Ruijuan,Chen Jing,Zhang Shuo,Allam Ahmed A.,Ajarem Jamaan,Wang Zunyao
Triclosan (TCS) is an antibacterial and antifungal agent widely used in personal care products, and it has been frequently detected in the aquatic environment. In the present study, the acute toxicity of TCS to Daphnia magna , Photobacterium phosphoreum , Danio rerio , and Limnodrilus hoffmeisteri was assessed under different pH conditions. Generally, TCS was more toxic to the four aquatic organisms in acidic medium. The LC 50 values for D. magna and D. rerio were smaller among the selected species, suggesting that D. magna and D. rerio were more sensitive to TCS. In addition, the oxidative stress-inducing potential of TCS was evaluated in Carassius auratus at three pH values. Changes of superoxide dismutase (SOD) and catalase (CAT) activity, glutathione (GSH) level, and malondialdehyde (MDA) content were commonly observed in all TCS exposure groups, indicating the occurrence of oxidative stress in the liver of C. auratus . The integrated biomarker response (IBR) index revealed that a high concentration of TCS induced great oxidative stress in goldfish under acidic condition. This work supplements the presently available data on the toxicity data of TCS, which would provide some useful information for the environmental risk assessment of this compound.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2020
·Vol. 45
·期 2
·e15139
·
IF 1.405
Yue-ming Hu,Nan-hai Zhang,Hui Wang,Yi-fan Yang,Zong-cai Tu
Pengze crucian carp was pre-frozen by liquid nitrogen and freezer, and then stored at −20/−40°C and −20°C, respectively, for 15–60 days. Effects of pre-freezing methods and storage temperatures on the qualities of fish were investigated. The pH showed a “V” trend. The soluble myofibrillar protein content, hardness, springiness, cohesiveness, gumminess, chewiness, and resilience decreased. Thiobarbituric acid value, total volatile basic-nitrogen value, thawing and cooking loss rates increased with the extending of storage period. LF-NMR analysis indicated that the water-protein connection became less tight. Moreover the qualities decline degrees were higher for freezer pre-frozen than liquid nitrogen pre-frozen, and higher for −20°C storage than −40°C storage. Accordingly, compared with conventional freezer pre-freezing and storage, liquid nitrogen pre-freezing and low temperature storage could inhibit the oxidation and degradation, and keep better water holding and textural properties of muscle. The present study showed a great potential application in aquatic products processing industry. Practical applications In aquatic product enterprise, liquid nitrogen can be used to pre-freeze fishes in seconds. And combined with low temperature storage, the oxidation and degradation of fish can be significantly inhibited. In addition, water holding capacity and textural properties of fish muscle can be better kept, and the shelf-life of fish can be extended by several weekends in comparison with those of fishes pre-frozen and stored in freezer.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）