MOLECULES
·
2019
·Vol. 24
·期 23
·4300
·
IF 3.06
Fu-Jia Chen,Xiao-Hua Long,En-Zhong Li
Phytophthora capsiciLeonian causes destructive economical losses in pepper production, and a promising source of natural fungicides-Helianthus tuberosusleaves was reported. The antifungal activities of different extracts and compounds fromH. tuberosusleaves against the phytopathogen,P. capsiciLeonian, were examined by chemometric analysis, including HPLC-MS/MS and multivariate data analyses. Principal component analysis and orthogonal partial least squares-discriminate analysis were applied to examine the four groups ofH. tuberosusleaves samples, including crude extracts obtained by different methods, including refluxing, macerating, and refluxing under vacuum; four fractions, namely, petroleum ether (PE), chloroform (Chl), ethyl acetate (EA), and n-butanol (NB) fractions; the samples of threeH. tuberosuscultivars; and the samples at three growth stages of cultivar Nan Yu. The phenolics contents were categorized based on 3,5-Dicaffeoylquinic acid (3,5-DiCQA), 1,5-Dicaffeoylquinic acid (1,5-DiCQA), 3-O-Caffeoylquinic acid (3-CQA), and 4,5-Dicaffeoylquinic acid (4,5-DiCQA), which were predominant in all the samples. Antifungal activity assay revealed that Chl and NB fractions were more active againstP. capsiciLeonian with lower IC50(half of maximal inhibitory concentration) values, whereas partial least squares-discriminate analysis suggested caffeoylquinic acid isomer(4-CQA), methyl-quercetin glycoside(MQG), and caffeic acid(CA) might be the main active components inH. tuberosusleaves againstP. capsiciLeonian. Furthermore, microscopic evaluation demonstrated structural deformities inP. capsiciLeonian treated with Chl and NB fractions, indicating the antifungal effects ofH. tuberosusleaves. These results imply thatH. tuberosusleaves with a high concentration of phenolics might be a promising source of natural fungicides.Keywords:Helianthus tuberosus;antifungal activity;phenolic acids;chemometric analysis;PLS-DA
研究领域:
植物科学
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植物化学
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真菌生物学
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分析化学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2024
·
IF 4.1
Zhongjian Tan,Yuwei Yan,Jiacong Liao,Huanhuan Shi,Yun Zheng,Weizheng Xu,Chen Yi,Zhiying Dai,Chenyang Xu
Droplet microfluidic technology can use each microdroplet as a microreactor, which has the advantages of low reagent dosage, less cross contamination, and fast reaction time. Combining concentration gradient generation with droplet formation and capture to form a two-dimensional reaction condition screening platform (including reactant concentration and reaction time) is expected to broaden the application range of microfluidic screening. In this work, a microfluidic chip that can dynamically generate and capture microdroplets and form static microdroplet array was designed and fabricated. An optimized Christmas tree structure by adjusting the horizontal channel length ratio was used to generate a chemical concentration gradient while obtaining a uniform outlet flow rate, forming a droplet array with different concentrations. The performance of droplet array with microfluidic concentration gradient (DA-MCG) was verified using sodium fluorescein as a model reagent. The chromogenic reaction of NaOH and phenolphthalein, and luminol chemiluminescence reaction were used to verify the two-dimensional screening ability of DA-MCG. The results indicated that the DA-MCG has the potential to be applied in the field of multi-dimensional drug screening.
研究领域:
微流控技术
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分析化学
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生物技术
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药学
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产品关联
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2024
·
IF 5.8
Chenyu Liu,Shuli Yue,Ruizhi Li,Lin Wang,Kun Zhang,Shiwei Wang,Simiao Yu,Farishta Shafiq,Yao Liu,Weihong Qiao
In response to the pain, swelling, and redness resulting from inadequate wound hemostasis and anti-inflammatory measures, there is a pressing need to develop wound repair materials capable of promptly stopping bleeding, facilitating painless dressing changes, and expediting wound closure to enhance skin wound recovery. However, comprehensive research is deficient concerning the conformational relationship of hyaluronic acid (HA) with various molecular weights concerning the structure of hydrogel materials. This study aims to address this need by utilizing aminated hyaluronic acid (AHA) and aldehyde-modified dextran (DA) to prepare dextran-based hyaluronic acid hydrogels (DA-AHA) via Schiff base reaction, imbuing them with acute inflammation repair and wound healing promotion properties. In vitro, coagulation experiments demonstrated that the DA-AHA hydrogel could rapidly coagulate blood within 60 s. Subsequent animal experiments corroborated these findings, revealing the hydrogel’s efficacy in significantly shortening wound healing time and accelerating wound closure efficiency. Moreover, MTT and live-death experiments affirmed the non-cytotoxic nature of the synthesized hydrogel. The study delved into the structural regularity and wound-healing efficacy of DA-AHA hydrogel materials featuring various molecular weights. Additionally, it compared the hemostatic and wound-healing properties of (high molecular weight dextran-aminated hyaluronic acid hydrogel) HDA-AHA, demonstrating its superiority in both aspects.
研究领域:
材料科学
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生物材料
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伤口愈合
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生物医学工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Qingbo Bao,Huijie Li,Youjie Rong,Jianhua Fei,Xiaomin Zhang,Zhuang Zhao,Jian An,Xiaobo Huang
Photocurable gels have broad application prospects in biomedicine, bionics, flexible wearable devices and other fields. However, there are still some problems in the current photocurable gels, such as notch sensitivity, that is, poor tear resistance. In this study, we provided a photocurable gel with excellent tear resistance. The gel prepolymer is mainly composed of hydroxymethylacrylamide (NAM) and cellulose nanocrystals (CNC) modified with dopamine hydrochloride (DA), referred to as DA@CNC. After photocuring, the prepared gels show excellent mechanical properties such as tear resistance, elasticity and toughness. The introduction of DA@CNC not only endows gels with a large amount of energy dissipation through hydrogen bond crosslinking, but also effectively resists crack expansion as a nano-sized reinforcing phase, which greatly improves the tear resistance of the gels. Even at a 40 % gap, the elongation at break of the gel can still reach 1445 %. In addition, the DA can endow the gel with good electrical conductivity and excellent sensitivity (GF = 23.8). Some flexible wearable devices like finger sleeve and wristband can be customized by photocurable 3D printing using the gel with high toughness. This high-performance gel has great application potential in flexible wearable devices.
研究领域:
材料科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
CELLULOSE
·
2022
·Vol. 29
·期 18
·9953-9967
·
IF 6.123
Wan Jiansheng,Li Hong,Cai Xiaoyu,Yan Jun,Liao Yongping
In this study, functional coatings with N-halamine structure were formed on cotton fabrics by dopamine (DA) auto-deposition and DA/Polyethyleneimine (PEI) covalent co-deposition, respectively. SEM、UV-Vis and XPS had confirmed the deposition of DA and DA/ polydopamine (PDA) on the cotton fabrics. When the mass ratio of DA/PEI was 1:1.5, a uniform functional coating was formed. XRD had indicated that the coating and the oxidative sodium hypochlorite solution did not affect the crystal structure of cellulose. The DA/PEI co-deposited modified cotton fabric had excellent antibacterial property after chlorination, and the inactivation rate against E coli and S aureus could reach 100% with a contact time of 30 min. In vitro cell cytocompatibility studies demonstrated that the DA/PEI co-deposited modified cotton fabric has good biocompatibility. The functional coating on the cotton fabric remained stable after 50 washing cycles. The UPF values and the wrinkle recovery angle (WRA) tests showed that the DA/PEI co-deposited coating imparted great durable press and UV protective properties to the cotton fabric. The breaking strength loss rate of the modified cotton fabrics after chlorination was about 20%, which had little effect on the wearing property. Graphic abstract
研究领域:
材料科学
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纺织工程
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生物医学工程
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DOI
高影响因子
Foods
·
2021
·Vol. 10
·期 11
·2709
·
IF 4.35
Yan Huang,Dandan Pu,Zhilin Hao,Xiao Yang,Yuyu Zhang
This work aims to explore the contribution of prickly ash (Zanthoxylum bungeanumMaxim) on the taste perception of stewed sheep tail fat. Liquid chromatography-tandem quadrupole time of flight mass spectrometry (LC-QTOF-MS) was applied to analyze the taste-related compounds. A total of 99 compounds in different sheep tail fat samples were identified. The semi-quantitative results showed that there were differences between the samples. The partial least squares discriminant analysis (PLS-DA) model without overfitting was used to investigate the effect of prickly ash. Eleven marker compounds were predicted with a variable importance for projection > 1, fold change > 2 andp< 0.05. An additional experiment showed that guanosine 5′-monophosphate, malic acid, inosine and adenosine 5′-monophosphate could improve the umami and saltiness taste of stewed sheep tail fat.Keywords:sheep tail fat;taste compounds;LC-QTOF-MS;PLS-DA;sensory analysis;additional experiment
研究领域:
食品科学
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化学计量学
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植物学
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DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 13
·期 1
·499-505
·
IF 4.036
Rong Wang,Jiamin Luo
Rapid and accurate identification of bacteria is essential to ensure food safety and prevent pathogenic bacterial infection. In this study, a highly efficient method was established for accurately identifying bacterial species by applying Ag NP-filter paper based Surface enhanced Raman spectroscopy (SERS) analysis and Partial Least Squares-Discriminant Analysis (PLS-DA) statistical methods. The flexible Ag NP filter paper substrate with high sensitivity and uniformity was prepared by a facile and low-cost silver mirror reaction at room temperature, which exhibited desirable SERS activity in bacteria detection. Furthermore, PLS-DA was successfully employed to distinguish SERS spectra from S. aureus CMCC 26003, E. faecalis ATCC29212 and L. monocytogenes ATCC 19115 with a sensitivity of 93.3–100%, specificity of 96.7–97%, and overall predicting accuracy of 95.8%. This exploratory study demonstrates that a Ag NP-filter paper based SERS sensor coupled with PLS-DA has great potential for rapid and effective detection and identification of bacteria.
研究领域:
分析化学
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微生物学
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食品安全
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2022
·Vol. 16
·期 1
·132-142
·
IF 3.498
Pu Keyuan,Qiu Jiamin,Li Jiaying,Huang Wei,Lai Xiaopin,Liu Cheng,Lin Yan,Ng Kwan-Ming
The problem of adulteration and mislabeling in meat products has raised the public concerns globally. An easy-operation, fast, and robust method that is applicable to routine inspections is urgently needed. This study showed that the MALDI-TOF MS protein profiling of four meat species (beef, chicken, duck, and pork) combining with partial least squares discriminant analysis (PLS-DA) discovered 57 feature peaks for their unambiguous differentiation. Among them, 36 were identified in Uniprot. Based on the linear relation between the intensities of feature peaks, the partial least squares regression was successfully applied to build the prediction models for determining the adulteration ratios of beef meat mixtures containing one of the other three species. Blind tests were applied to evaluate the method and the average prediction accuracy at 94.7% was achieved. Taking duck meat as the adulterant, the detection sensitivity of the method could be down to 5%. Moreover, the method has also been successfully applied to analyze market samples and the results were in agreement with the PCR method, showing the potential of its practical application for qualitative and quantitative analysis of adulterated beef products.
研究领域:
食品科学
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生物化学
·
分析化学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Kexin Kang,Situ Mu,Jianrui Niu,Yongkun Shi,Jing Zhang,Chun Liu,Junjun Ma
Natural brackish water bodies constitute complex mixtures. Accordingly, the production of high-quality drinking water from such sources necessitates the concurrent removal of salts and natural organic matter (NOM). A dual-anode circuit system (DA-FCDI) was developed by integrating electrochemical oxidation with flow-electrode capacitive deionization (FCDI). This dual-circuit configuration enables modulation of both desalination and electrochemical oxidation performances, while simultaneously regulating the charge and energy distribution between these two processes. At the same time, it was found that an increase in voltage difference between the internal and external circuits is beneficial for the degradation of organic matter. Furthermore, I − /I 3 − redox electrolytes were employed to expand the voltage window, thereby overcoming the voltage threshold limitation of the DA-FCDI system and enhancing its overall performance. When the applied voltage is 1.2 V - 1.8 V, ASRR and ATRR are 0.059 μmol cm −2 min −1 and 0.34 μg cm −2 min −1 , respectively, and the specific energy consumption is only 0.052 kWh/m 3 .Compared to other technologies, this FCDI and electrochemical oxidation integrated device significantly reduces the consumption of chemical agents and simplifies the process flow. More importantly, while improving economic efficiency, it endows the system with high adaptability and controllability to different water qualities. This study breaks through the bottleneck of traditional single technology that cannot regulate and remove salt and organic matter separately.
研究领域:
环境工程
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水处理技术
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电化学工程
·
脱盐技术
·
污染物去除
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产品关联
DOI
高影响因子
TOXICOLOGY
·
2026
·
IF 5.2
Yiming Pang,Zhenzhong Wang,Yijun Liu,Chuanteng Feng,Yuchun Song,Shannan Cao,Dong Wang,Lianshuang Zhang,Peng Lu,Li Yu
Hexafluoropropylene oxide dimer acid (HFPO-DA; GenX) is an environmentally persistent per- and polyfluoroalkyl substance (PFAS) alternative whose placental toxicity remains poorly understood. Pregnant Sprague-Dawley rats (n = 20, 5 dams/group) received oral HFPO-DA at 0, 1, 10, or 100 mg/kg·day from gestational day (GD) 0–19. Placental tissues collected on GD20 were analysed by targeted metabolomics, lipidomics, histopathology, immunohistochemistry (IHC), and immunofluorescence (IF). After false discovery rate (FDR) correction, three energy metabolites were significantly altered ( q < 0.05): oxaloacetate and glutamate were downregulated, while pyruvate was dose-dependently upregulated, consistent with restricted TCA cycle entry. Lipidomics identified 148 nominally significant lipid species, with elevated ceramides and long-chain fatty acids alongside reduced triglycerides, a pattern suggestive of impaired mitochondrial β-oxidation capacity. Histopathology revealed dose-dependent villous hyperplasia, trophoblast disorganization, and vascular lesions at the high dose. Protein analysis showed decreased SDHB, increased HIF-1α and PDK1, and a non-significant rise in PDHA1, collectively consistent with a shift toward Warburg-like metabolic reprogramming. These findings suggest that gestational HFPO-DA exposure may disrupt placental energy and lipid homeostasis at supra-environmental doses, identifying placental metabolism as a potentially sensitive target of next-generation PFAS.
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
Luying Liu,Yue Luo,Wei Li,Chunmeng Dong,Shiyu Lou,Sheng Dai,Hongbo Wang,Jingmei Pan,Lang Jiang,Guicai Li,Ansha Zhao
Stenting is a common and effective therapeutic strategy for atherosclerosis. However, a series of adverse reactions can occur after stent implantation, such as inflammation, vascular smooth muscle cells (SMCs) proliferation, and delayed endothelial repair, all of which are closely related to the oxidative stress microenvironment. Here, we propose a method for the copolymerization of copper porphyrin (CuTPPS) with dopamine (DA) that can easily produce a catalytic antioxidant coating on the stent surface. By rapidly decomposing H 2 O 2 , the optimized Cu10 coating reduced the level of intracellular oxidative stress and protected Endothelial cells (ECs) from H 2 O 2 damage. The Cu10 coating has been demonstrated to have superior anticoagulation and anti-inflammatory properties, promoting the transition of SMCs from the synthetic to contractile type, thereby inhibiting intimal hyperplasia. Hence, this catalytic antioxidant surface is an effective solution for the complications of stents and other vascular-implanted devices.
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Boxuan Yang,Yuxin Zeng,Tao Guo,Yuanzhong Lin,Lifeng Yin,Qingsong Hua
Hexafluoropropylene oxide dimer acid (HFPO-DA), an emerging persistent pollutant, has been prevalently utilized as an alternative to perfluorooctanoic acid (PFOA). Analogous to PFOA, HFPO − DA presents remarkable resistance against degradation by conventional technologies. In this study, we prepared Ce doped PbO 2 anode (Ce-PbO 2 ) and paired it with cathode to form redox system for the degradation of HFPO-DA in water. The results showed that the degradation rate of HFPO-DA over Ce-PbO 2 was as high as 1.67 × 10 −2 min −1 , much higher than that of Boron-doped diamond electrode (BDD, 6.84 × 10 –3 min −1 ). The Ce-PbO 2 anode also has the theoretical lifetime as high as 82.7 years. Free radical quenching experiments indicated that both reactive oxygen species (ROS) and reductive radicals contributed to the degradation of HFPO-DA. These degradation reactions start from a direct electron transfer, followed by electroreduction and a series of free radical reactions including decarboxylation, ether bond cleavage, and the “4A” processes (generation of Alkyl radicals, Alcohols, Acyl fluorides, and Acids in sequence) until it is decomposed into small molecules. Meanwhile, new intermediates (CF 3 CF 2 CF 2 OCF 3 CH•) generated by cathodic H• are dominating the reductive reaction during the electrochemical process. This “buttering bread on both sides” redox mechanism deepens the understanding of the degradation process of HFPO-DA and provides an efficient treatment strategy.
研究领域:
环境科学
·
电化学
·
污染治理
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）